LOCUS       DASCTH010000001         3761 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000000298, whole genome shotgun sequence.
ACCESSION   DASCTH010000001 DASCTH010000000
VERSION     DASCTH010000001.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000000298"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="QGH35_00005"
     CDS             complement(<1..836)
                     /locus_tag="QGH35_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HJL80948.1"
                     /translation="MEYTDTYEGQRVSDAKSWITKYVFCQDHKVIAIQYSCVAMFTGI
                     VGVILSILMRLQLGYPDVFGITAAEYYQHTTMHGMIMVIYLLTALFLGGFGNYLIPLM
                     LGARDMAFPFVNMLSVHFFTLSVIILMGSYIVPGGPTGAGWTLYPPQVLLPGTPGYDW
                     GIIMMILSVAVFIIAFTMGGLNYVTTVLQSRTRGMTLMRMPLVIWCIFLATVIGLLAF
                     PALLVAGVMLLLDRVVGSSFFMPDVFQAGQHVTNVFGTEYEGGSPLLFQHLFWFFGHP
                     EV"
     gene            complement(884..2005)
                     /gene="coxB"
                     /locus_tag="QGH35_00010"
     CDS             complement(884..2005)
                     /gene="coxB"
                     /locus_tag="QGH35_00010"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HJL80949.1"
                     /translation="MLVTLIFLLFTIAVVAFHFYALEQGFWFPVLASNWALMDLTTKI
                     TLAVSAVVFIVISVFVCYCIFRFRRREGHSADHEPENSKENSKLEGWLTIFTTVGVIA
                     LLSPGLMVYRDFINPPADAIKAEVLGEQWTWSYRFPGNDGIYGRTNPKFFSLDNSFGL
                     DPSDPASQDDVLVNTNTVHFPINQPVDIQLRSKDVLHDFYIPQFRAKIDMVPGQVTKL
                     WFEPTLEGEYEIVCAEYCGIAHYNMRGVAIVNSVPDFQFWLDQQQTFGEFQQQAASSS
                     GGVTDKDGELIARTKGCIACHSIDGKPGIGPSWKGIAGEDRVLVGGGSMIANDLYLKR
                     SITNPNDEVVLGYAPMMPPYPLSEEELDAIVEYINSLGE"
     gene            complement(2248..2934)
                     /locus_tag="QGH35_00015"
     CDS             complement(2248..2934)
                     /locus_tag="QGH35_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012142241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL80950.1"
                     /translation="MFAFLRDSYGHLPLGQIESVFGFVERSSLYGGRHFMSPELSNRD
                     VHQLNNAGIGLRIPFTNHFVDYEEYQANKPLISKYHNEINSVICTNDELATWLREDFP
                     KFCHVEASVIKNIDNTSKLEKALGIYTCAVLPMTSNDDLDFLDSITEKARIRLFANGG
                     CAYTCPSKICYRSQSKLNKFKGGEFKCSQPIKKRELIGMINFDLELLTNKGFNKFKLL
                     RARPGQMTGH"
     gene            complement(3198..>3761)
                     /locus_tag="QGH35_00020"
     CDS             complement(3198..>3761)
                     /locus_tag="QGH35_00020"
                     /inference="COORDINATES: protein motif:HMM:NF039236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL80951.1"
                     /translation="DHPTSEGTTPWGDTKTERSPVTAGGGTHGPNTLITHINITPETG
                     FRQYFFEQPISETKTKIFFVNNRNFLMDPSMDEPIHARNLVIAQQDIDILIDVYPTVT
                     PFVVKEEVIVPADVPIVAYRDWLAKFDAKGWRIDTKEFNENNGKGKAYAIPGPGRNKS
                     KNYPLDTLPLVEASDDSDPAVMRLRTG"
BASE COUNT         1118 a          846 c          739 g         1058 t
ORIGIN      
        1 tagacttccg ggtgtccaaa gaaccaaaat aaatgctgaa acaacagtgg actgccacct
       61 tcatattcgg taccgaaaac attggttacg tgttgtccgg cttgaaaaac atccggcata
      121 aaaaaactac tgccaacaac acgatcaaga agtagcatga ccccagcaac caacagtgct
      181 ggaaaggcta aaaggccaat gacagtggca aggaagatgc accagatcac taaaggcatc
      241 ctcatcaagg tcatcccgcg agttctggat tgcaataccg tggtgacgta atttaggcca
      301 cccatggtaa atgcaataat gaatacagcc accgagagga tcatcatgat aataccccaa
      361 tcatatccag gagtgcctgg taacagcact tgaggagggt acagtgtcca accagccccg
      421 gttggccccc ctggaacaat ataactgccc atcagtatga tcactgaaag tgtaaaaaaa
      481 tgaacactga gcatatttac aaatggaaat gccatgtctc gtgctcccaa catcaaaggg
      541 atgaggtagt tgccaaagcc gcctaaaaac aaggctgtca aaaggtatat gaccatgatc
      601 atgccgtgca tggtagtgtg ctgataatac tcagctgcag tgatcccgaa tacatctgga
      661 taaccaagct gcagacgcat taatattgat aatataactc caactatgcc cgtgaacatt
      721 gccacgcatg aatactgaat cgctattact ttatggtcct ggcagaatac gtattttgtt
      781 atccaactct ttgcatcaga aaccctctga ccttcatagg tgtcagtata ttccatggca
      841 aactccctcc tttttagttt taaccattaa tttcatcatc aaattattca cctagactgt
      901 tgatgtattc cacgattgca tccaactctt cctcggataa tggatatgga ggcatcatcg
      961 gggcataacc gagaacgact tcatcgtttg gattggtaat tgatcgtttc aaataaagat
     1021 cattagcaat catagatcca cctccaacca agactctgtc ctcacctgca atgcctttcc
     1081 atgatggacc gatcccaggt ttaccatcaa tactatggca tgctatacat ccttttgttc
     1141 tcgcaatcaa ttcaccatcc ttatccgtaa cgccacctga tgatgaggct gcttgttgct
     1201 gaaactcacc gaaggtttgt tgttgatcta gccagaattg aaaatcgggc accgagttta
     1261 caattgctac gccacgcata ttgtagtgtg cgataccaca atactctgcg catacgattt
     1321 catattcgcc ttctaatgta ggttcaaacc atagttttgt aacttggccc ggaaccatgt
     1381 ctattttggc tctaaattgt ggaatgtaaa aatcatgcag cacatctttt gatctgagtt
     1441 gaatatctac cggttgattg atcgggaaat gaaccgtatt ggtgttaacg agaacatcat
     1501 cctgagatgc tggatccgat ggatccagac caaatgagtt atcaagacta aagaacttgg
     1561 gattggtcct gccataaatg ccatcatttc ccggaaaacg gtagctccat gtccattgct
     1621 ccccaagaac ttctgctttt atggcatcgg caggagggtt gataaaatca cgatagacca
     1681 tcaatccagg tgaaagaagt gcaataaccc caacggttgt aaaaattgtc aaccaccctt
     1741 caagtttact gttttcttta ctgttttccg gttcatgatc ggcgctgtgt ccttctctgc
     1801 gacgaaaccg aaaaatgcag taacacacaa aaacactgat gactataaag acaacagcac
     1861 tgactgctaa ggtaattttg gttgttaaat ccatcaatgc ccagtttgat gccaatactg
     1921 ggaaccagaa accttgctct agggcataga aatgaaatgc tacaactgcg atggtaaaga
     1981 gtaagaaaat caatgtaaca agcatattgg ttccaagttt ttttatggtg ctcttttgat
     2041 attaattctg gataaaatga tattttgaac aacatctatt ttcactgatc tagatcatga
     2101 ggcacttgat ctatatcaat agtcacttga tctagatcaa tttgaccagt caaccagaaa
     2161 aatggattaa aaaaaagatt tggataataa gcctacccga tgggtgtcaa tcatccacga
     2221 aacattcaaa acacctggtc tatcttatca gtggcccgtc atttggcccg gtctggctct
     2281 caagagttta aatttattaa atcctttatt agttaaaagc tccaaatcaa agttaatcat
     2341 tcctatgagc tctctttttt ttatcggctg ggagcattta aattcaccgc ctttgaattt
     2401 attgagtttt gattgagagc gataacagat ttttgacggg catgtatacg cacaaccgcc
     2461 gttggcaaat aaccggatcc ttgccttctc agtgatagaa tctagaaaat ctaaatcatc
     2521 attgctggtc attggcagaa cagcacacgt atagatgcca agggcttttt ctaacttcga
     2581 tgtgttatct atatttttta taacgctggc ttcaacatgg cagaattttg gaaaatcctc
     2641 cctcagccac gttgctaatt catcatttgt gcaaatgacc gaattgattt cgttgtggta
     2701 ttttgaaatc aatggtttat tcgcctggta ctcctcataa tcaacaaagt gattagtgaa
     2761 aggaatcctt aaaccaatgc cagcattatt aagctggtgc acgtctctgt tggagagttc
     2821 aggactcata aaatgacgac ctccatacag tgaggatcgc tcaacaaaac caaaaacgct
     2881 ttcaatttga ccgagaggca aatggccgta tgagtccctc aaaaaagcaa acatcggctt
     2941 atttggactt ttacctctgg cagacaccgt atacgagacg gaatcatctc tactggatac
     3001 cgaatttgaa ttgctatcaa gcaaaggcac gcccttctat cttgttaatt tcccctaatg
     3061 aattaataag attctataga agattaatca gtaatcaaat attgccgatc ataatcggca
     3121 gtgcaccggg tttaggtaat aggggtaacc tagcccaaat gcactgcttt ctaccaaatc
     3181 aaaccgctct tatactctta accagttctt aaccgcatta ctgcaggatc cgaatcgtca
     3241 gatgcttcga ccagtggtag cgtgtcgagt gggtagttct tggacttgtt cctgcctggg
     3301 ccagggatgg cgtaggcctt gcctttacca ttgttctcat tgaactcctt ggtatcaatc
     3361 ctccaaccct tagcatcgaa tttagccaac caatcgcggt aggcaacaat aggaacgtct
     3421 gcagggacaa tgacttcctc tttcactacg aaaggagtca ctgttggata aacatcaatg
     3481 agaatatcaa tatcctgttg agcaataacg agattccgtg catgaatcgg ctcatccata
     3541 ctgggatcca tcaaaaaatt acgattattc acaaagaaaa tctttgtttt cgtctcacta
     3601 atcggctgct caaagaagta ttgcctaaac cctgtttcag gagtgatatt gatgtgcgtg
     3661 atcagggtat ttggaccatg agttccgccc cctgctgtaa caggacttcg ttccgtcttg
     3721 gtatctcccc atggggttgt gccttctgat gtcggatgat c
//
LOCUS       DASCTH010000002         2662 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000001005, whole genome shotgun sequence.
ACCESSION   DASCTH010000002 DASCTH010000000
VERSION     DASCTH010000002.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000001005"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(98..1807)
                     /locus_tag="QGH35_00025"
     CDS             complement(98..1807)
                     /locus_tag="QGH35_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HJL80952.1"
                     /translation="MSSNMPPEFDAIVVGSGISGGWAAKELTERGLKVLLVERGPHIT
                     HRKDYSGEGKAPWEMPFAGRIPEDEANRDYFVQKDCYALESATKKFFVNDRIHQYIHP
                     QEKPFSWIRGYQLGGRSQLWARQTYRWSDIDFRANKTDGHGIDWPIRYQDIAPWYDHV
                     EQFVGISGSLEGLDQLPDGKFLPPMTMNCVEAEAKQKIEKAFPTRKMIIGRCANLTKP
                     TKEHLELGRGRCQYRNECEKGCSFGASFSSLSATLPAARRTSNLTTVTDAIVHSVIYD
                     DVSKRATGVKIIDANTKDYHQYSGRIIFLCASTLGSTQIMLNSISESFPNGIANSSGT
                     LGHYLMDHIFMSGASGQHPGFQEHYYSGRRPNGIYIPRYRNVTEKDDRFIRGYGFQGG
                     ASRPNWRRGLNMQGIGLELKEKLRVPGSWQFNLGGFGEMLPDKRNRVLLDKNQQDQWG
                     IPLLNIECELRDNELKMLDAMSDDAEAMLSTAGIINIQKYNEDAPPGLAIHEMGTARM
                     GHNPSSSVLNKFNQTHDVSNLFVTDGSAMTSSACQNPSITYMALTARASAYAVQLLKA
                     GTI"
     gene            complement(1828..2418)
                     /locus_tag="QGH35_00030"
     CDS             complement(1828..2418)
                     /locus_tag="QGH35_00030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HJL80953.1"
                     /translation="MDKNITLNSITFTRRKAIRNAVLLLGGSISAAQISPLISNVTAM
                     DANYIPKFLSQHHFQMTQHLVDLIIPESDTPGALTANVHQFIDVMLDGWAASDTQLRF
                     LDNFNNIDRRSATMTGKTFANANRTQQIKLLEVLDKESFSDNGTDIFFGEFKALVIFG
                     YYSSAEGASIELRYDRIPGDYRDCIPFSEVGRSWST"
     gene            complement(2435..>2662)
                     /locus_tag="QGH35_00035"
     CDS             complement(2435..>2662)
                     /locus_tag="QGH35_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HJL80954.1"
                     /translation="GNIVKNLYAGIDQVGYIQVADIPGRHQPGSGELNYNFIFHTIDD
                     MGYKGPIGLECWPENNDEKQAIADIIVQRFG"
BASE COUNT          749 a          585 c          487 g          841 t
ORIGIN      
        1 tttccattgg cgttattaca cccatacaga agcacagtcc cagctattgc aagcaaaaaa
       61 tcacgcctca atatggtttg attctgattt ttgaacttca aattgttcct gcttttaaga
      121 gttgcacagc atatgctgaa gctcttgctg ttaaagccat ataggtgatc gatggatttt
      181 ggcaagcaga tgaggtcata gctgagccat cggttacaaa aagatttgat acatcatggg
      241 tttgattgaa tttatttagt accgagctgc tcggattatg gcccattcga gccgtaccca
      301 tctcatggat agcaagccct gggggagcat cttcattgta tttttgtata ttgattattc
      361 cagcagtact taacatagct tctgcatcat ccgacatggc atcaagcatc tttaattcat
      421 tgtcacgaag ttcgcattct atattcaata atggtattcc ccattgatcc tgctggtttt
      481 tatccaaaag tacacgattt cttttatcag gaagcatttc accaaaacca ccaagattga
      541 attgccaaga accaggtact cttagttttt cttttaattc aagaccaata ccttgcatat
      601 tcaaaccgcg tctccagttc ggtcttgagg cgcccccttg gaaaccgtaa ccgcgtataa
      661 aacgatcatc cttctcggtc acattcctat atcttggaat ataaatccca ttaggtcttc
      721 tgcccgaata atagtgttct tgaaatccag ggtgttgacc acttgctcct gacataaaaa
      781 tatggtccat aagataatgc cccaaagtgc cactggaatt agcaataccg tttggaaaac
      841 tctctgatat cgaattcaac atgatttggg tggagcctaa tgtagaagca cagagaaata
      901 ttatacgtcc agaatactgg tgatagtctt tagtgtttgc atcaataatt ttaactccag
      961 tggctcgttt tgagacatcg tcataaatca cactatgaac aattgcatca gtgacggtag
     1021 ttaaattact tgtcctgcgt gcggcaggta gtgttgcgga aagtgagctg aaggatgcgc
     1081 cgaacgaaca tcctttctca cactcattgc gatactggca acgaccccga cctagctcaa
     1141 gatgttcttt agttggtttc gtaagattag cacaacgtcc tataatcatc ttacgtgttg
     1201 gaaaggcttt ttctattttt tgttttgcct cagcttcaac gcaattcatt gtcattggag
     1261 gtaaaaattt cccatctggt aattgatcta gcccttctaa ggagccactg attcctacaa
     1321 actgttcaac atggtcatac catggagcaa tatcttgata acgaataggc caatcaatcc
     1381 cgtggccatc agttttgtta gctctgaaat cgatatcact ccaccggtaa gtctgcctag
     1441 cccagagttg cgatctacca cccagttggt aacctctaat ccaactaaat ggcttctctt
     1501 gaggatgaat gtattgatgt atccgatcgt taacaaagaa tttcttggta gcactttcta
     1561 aggcataaca gtctttttga acaaaataat cccgattcgc ttcatcttct ggaatacggc
     1621 ctgcgaacgg catttcccat ggggctttac cttctccgct ataatctttt ctatgagtta
     1681 tgtgcggacc tctctccaca agcagtactt ttaaacctct ctcagtaagc tcttttgcag
     1741 cccatccccc tgagattccc gaacccacta ctatggcatc aaactcaggt ggcatattac
     1801 tcgacattaa tcattccctt ataaacatta ggttgaccaa ctgcggccta cctcgctgaa
     1861 tgggatacag tctctgtaat cacctggaat acgatcgtat cgcaactcaa tggaagcgcc
     1921 ttctgctgat gaataataac caaaaataac caaagctttg aactctccaa aaaaaatatc
     1981 agtgccgtta tcggaaaatg attccttgtc taatacttca agtaatttta tttgctgtgt
     2041 tctattagca ttggcaaatg ttttaccagt catggtcgca ctacgacgat caatattatt
     2101 aaaattatcg aggaaacgaa gctgtgtgtc tgatgctgcc cacccgtcca acataacatc
     2161 aataaattga tgtacattgg cagttaatgc acctggagta tcggattctg gaatgatcaa
     2221 atcaactagg tgttgagtca tttgaaaatg atgttggctg agaaattttg gtatgtaatt
     2281 tgcatccatt gcagtcacat tgctgattaa agggctaatt tgggcagcag atatacttcc
     2341 gcctaaaagc aatactgcat ttctaattgc ctttcttctt gtgaatgtta ttgaatttaa
     2401 tgttatgttt ttgtccatat ttaatttaat aaaattaacc gaagcgctgc acaataatat
     2461 ctgctatagc ttgcttttca tcattatttt ctggccaaca ttctagtcca atcggtcctt
     2521 tatagcccat gtcatcgatg gtatggaaaa taaaattata attcaattct cccgacccag
     2581 gttgatggcg tcctggaata tctgcgactt gtatataacc aacttggtct attccagcat
     2641 aaagattttt cactatattg cc
//
LOCUS       DASCTH010000003         3549 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000005977, whole genome shotgun sequence.
ACCESSION   DASCTH010000003 DASCTH010000000
VERSION     DASCTH010000003.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000005977"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="QGH35_00040"
     CDS             <1..1366
                     /locus_tag="QGH35_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HJL80955.1"
                     /translation="TRAFENAQTIYTPAVHPREPYISQRELFVSPFYSREKELGGYFD
                     NEVAGWERALAYESNRQKLDNYLQEVPVRENEWDRRHVPYEIANAEHLAMSESAGMIN
                     LSHFAIIDIKGSDTERMLEYLSVAKIGGKTSEGRIIYTNFLDEDGGVHADLTISRLGT
                     DRYRIVTGGADGNRDWVSLRNYRDDLGLEADINIRTHDIATLGLWGPRAGDALGHFID
                     PSEISIENFPFVAAKYLTLNLSGGKTINVWAARISYVGESGWEIYLNNDSEEGLALYD
                     SLLEVGVVPVGIETYANSRRLEKSFRLQGADLETEYNACESAVERPKVKAANFHGKAA
                     HLAHREEEPSAILCTMTLDNLNVSGSGSRYPVGISPIIDTDTGEVPVDSKGRRSYSTS
                     MSYCPSIKKHVVMGYLPKNIAIPGKSLSLEYFNENGDGIYPMTVQIVGKGSLYDPNNE
                     RVRS"
     gene            1385..2926
                     /locus_tag="QGH35_00045"
     CDS             1385..2926
                     /locus_tag="QGH35_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HJL80956.1"
                     /translation="MPERKHRRQAGGRRNKIAAREQSARKYRPYIERHIPYFEILNEE
                     ELALIEKNADILLEEIGIEFRDFPKALDLFKNAGADIDDECVHFPPGFCREIIQSNAP
                     REYIQHARNPANNVIIGGRRTVLVPAYGSPFVRDLDKGRRYATIEDFRNFVKLAYMSP
                     NLHHSGGTICEPVDVPVNKRHFDMIYSHIKYSDKPFMGSVTHPERAEDTVSMAKLVFG
                     EKFLEENAVLTSLINVNSPLTFDDTMLGSATVYAEHNQATMISPFILAGAMSPVSIAG
                     TVTQILAEALAGMAYIQLLKPGAPVIFGAFASAVSMQSGAPTFGTPEPGHVMTIVGAL
                     ARRLGVPFRSGGGLCASKIPDAQAAYESANTMQASALAGVNFMLHTAGWLEGGLSMGY
                     EKFIMDADQAGMLAVMLEGVELSENAQAMDAFREIGPGGHFLGAAHTQANFESAFYRS
                     ELADNKTFEQWEEEGSLDCAMRANLKWKAMLKEYEAPKLDPAIDEALLEFIAKRKSEF
                     EDRDY"
     gene            3047..>3549
                     /locus_tag="QGH35_00050"
     CDS             3047..>3549
                     /locus_tag="QGH35_00050"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HJL80957.1"
                     /translation="MNTSKPSAAVGKRIQEDLENAYMEIIPIPGIEERLGTLQPNSHV
                     AVTCSPTKGVDETIELSEKLIAQGFQVIPHIAAKCVSGEKHLETIVKRLDALSIESIF
                     VPGGDRPEPIGEFNNAYDLLKALQKSGHNIKNIGIAAHPEGHPDVNEKILMEALEKKK
                     DLADYIVT"
BASE COUNT         1091 a          700 c          831 g          927 t
ORIGIN      
        1 aacacgagct tttgaaaatg cacaaaccat ttatacccct gctgttcacc ccagagagcc
       61 ctacattagc caaagagaac tattcgtcag tcccttttat tcaagggaaa aagagcttgg
      121 gggctatttt gataacgaag tcgccggctg ggaacgtgcc ttagcctatg agagcaatcg
      181 acaaaaactc gataattatt tgcaggaggt cccggttcga gaaaacgaat gggatcgacg
      241 ccacgtaccc tacgaaatcg ctaatgcaga acatctagcc atgagtgaat cggctgggat
      301 gatcaatcta tcgcactttg cgatcatcga catcaagggt tcagatacag aacgtatgtt
      361 ggagtatctt tccgtggcga agattggcgg caaaacctct gaagggcgaa tcatctacac
      421 taatttcctg gacgaggatg gtggtgtcca tgcagacctg accatctccc gcttaggtac
      481 agatcgttat cggatagtta ccggtggcgc cgatggcaac cgagattggg tgagcttgcg
      541 caattatcgg gatgatctgg gactggaagc agacatcaac attcggaccc acgatatagc
      601 aactttagga ttatggggac ctcgggcagg agatgccctg ggtcatttta ttgatcccag
      661 tgaaatcagc atcgagaatt ttccctttgt tgcagcaaaa tacctgaccc tcaatttatc
      721 tggcgggaaa acgatcaatg tctgggcagc acgcatttcc tacgtcggag agagcggctg
      781 ggaaatctat ttgaacaatg acagtgaaga gggcctagca ctttatgact ctctacttga
      841 ggttggagtt gtgccagttg gtatcgaaac ttacgccaac agtcgacgcc ttgaaaagag
      901 ttttcgactt caaggtgcag atctagagac cgaatataat gcttgtgaat cggctgttga
      961 aagacctaag gtcaaagcag cgaacttcca tggtaaagca gcgcaccttg ctcatcgaga
     1021 agaagagcct agtgcaattc tatgcaccat gacgttggat aacctaaatg tgtcgggtag
     1081 cggttcacgc tatccggtcg gcatctcacc aatcattgat actgatacag gcgaagtgcc
     1141 ggtagatagc aaaggccgca ggtcctacag taccagcatg tcgtattgcc catcaatcaa
     1201 aaaacacgta gtcatgggtt acctgcctaa aaacatcgca ataccaggaa agtcactgtc
     1261 gctggagtac tttaacgaaa acggtgatgg catttatccc atgaccgttc agatagttgg
     1321 caagggctct ctctatgatc ctaataatga aagggttcgt tcttaactgg agcaagataa
     1381 acttatgcca gaaaggaaac accgaagaca agctggtgga agaagaaata aaatagcagc
     1441 cagagagcaa agcgcaagaa aatatcgacc atatattgag cgccatattc cgtattttga
     1501 aattttaaat gaggaagaac tggctttaat agaaaagaat gccgatattc tacttgagga
     1561 aattggaatt gagttccgtg atttcccgaa agctctagat ttatttaaga atgctggtgc
     1621 agatattgat gatgagtgcg ttcattttcc gccaggattt tgtcgagaaa tcattcaatc
     1681 caatgcccca agagaatata tacaacacgc aaggaatcca gctaataatg ttattattgg
     1741 ggggcgaaga acagttcttg ttccagctta tgggtcacca tttgttagag atttagataa
     1801 aggtcggcga tacgcaacaa ttgaagattt tagaaatttc gtaaaattag catacatgag
     1861 cccaaatctt caccactcag gtggaactat atgtgagcca gttgatgtac ctgtcaataa
     1921 acgtcacttt gacatgatat acagtcatat caaatacagc gataaaccat ttatgggatc
     1981 ggtaacacat ccggaaagag ctgaagatac tgtgtcaatg gcaaagctag tttttggcga
     2041 aaaatttctc gaagaaaatg ctgtcttaac cagcttgatt aatgttaatt cacctttaac
     2101 gtttgatgac acaatgcttg gttctgccac tgtctatgct gagcataatc aagcaacaat
     2161 gatttcaccc tttattctgg ctggagcaat gtctccagtt tccatagccg gtactgtgac
     2221 acagattctt gctgaagcac ttgcaggcat ggcttatatt caacttctaa aaccaggggc
     2281 tccggtaata tttggggcat ttgcaagcgc agtttctatg caatcaggag cccccacatt
     2341 tggcactcca gagcctggtc atgtaatgac gattgtgggt gcgcttgcta ggcgattagg
     2401 cgtgcccttt cgaagtggtg gtggattatg cgcatcaaaa attccagatg cacaagctgc
     2461 ttatgaatca gctaatacaa tgcaagcttc tgccttagcg ggagtcaatt ttatgctgca
     2521 cacagccggc tggcttgagg gcggtctttc tatggggtat gagaaattta taatggatgc
     2581 agatcaagcg ggaatgcttg ctgtgatgct tgaaggtgtt gaattatctg aaaatgctca
     2641 agcaatggat gccttcaggg agattggtcc aggcggtcac tttttaggtg cggcacatac
     2701 acaagctaat tttgaatctg ctttttatcg gtctgaactc gctgataaca aaacttttga
     2761 acaatgggaa gaagaaggaa gtttagactg tgcaatgcgc gccaatttaa aatggaaagc
     2821 aatgttaaaa gaatacgaag ctccaaaatt ggatcctgca attgatgaag ctttacttga
     2881 gtttatagct aagagaaaat ctgaatttga agatagggat tattgattta aatcattaag
     2941 aagtagtttc aaaatcacgg cgttaaaact tctggaaaaa ttaaacagac aaaataaata
     3001 agatactttt cttaggtata gaaaatcagc ttggtaggag aaaattatga acacgagcaa
     3061 accttcagca gctgtcggta agcgcataca agaggatctg gaaaacgcct atatggaaat
     3121 tattccaatt ccaggcattg aagagaggct cgggactctt caaccaaatt cgcatgtggc
     3181 tgttacatgc tcgccgacaa aaggtgtaga tgaaaccata gagctaagcg aaaaacttat
     3241 cgcacaaggt tttcaggtta ttccgcatat tgcagcgaaa tgcgtaagtg gggaaaaaca
     3301 tcttgaaact atcgtcaaaa gactcgatgc attgagtatt gagagtattt ttgtgcctgg
     3361 cggcgaccga cctgaaccaa taggtgagtt taataatgcc tatgatcttc ttaaagcttt
     3421 gcagaaatct ggtcataata taaaaaatat tggaattgct gcgcatcctg aggggcaccc
     3481 agatgttaac gagaaaatct taatggaagc acttgaaaag aaaaaagatt tagcagatta
     3541 cattgttac
//
LOCUS       DASCTH010000004         5924 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000006233, whole genome shotgun sequence.
ACCESSION   DASCTH010000004 DASCTH010000000
VERSION     DASCTH010000004.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000006233"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(21..923)
                     /locus_tag="QGH35_00055"
     CDS             complement(21..923)
                     /locus_tag="QGH35_00055"
                     /inference="COORDINATES: protein motif:HMM:NF039482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HJL80958.1"
                     /translation="MTTFKNIIFAANSHDNNSMEALNIILDELNHQELQIGFYDFKLD
                     GDSSSTEISNKAQEIKKEDLPNNDLIVSIGGDGTMLKSAKLAHQYKIPITGINRGRLG
                     FLTDINPDQAGETLIKIINGVYKEEKRLLIEIRVNGKDKDCFSGYALNDIAIKRIETG
                     RMIRIKTSIDNNYINTHEGDGFVVATPTGSTAYALSCGGPILKPDIEAFVLVPICPHS
                     LNDRPLVIPAHSEINVTVDLNDDETAAIDLDGDDFTELSSEDILSIRASSTFIKLIHP
                     ENYDYFDVLRTKLYWGQDKRTAIG"
     gene            1030..2082
                     /gene="hrcA"
                     /locus_tag="QGH35_00060"
     CDS             1030..2082
                     /gene="hrcA"
                     /locus_tag="QGH35_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HJL80959.1"
                     /translation="MKEYNVDSSPSLRAKLILSAIVDKYVQDGTPVGSKSLSLTDSID
                     LSPATIRHVMSDLEELGFIVSPHTSSGRIPTPKGYRFFIDSLLKLQPVEQNEIQTIKA
                     RVELSKNSGRELAKSVSSTLSAITKLAGIVTIPKQSVTRLKEMDFIPLSDKKILAIIV
                     TNEAEVENRILEMNKVYSREELNRAANYLNTNFRGRSLVYIKKQLIEELRTTKESVTA
                     MMTDLINIADQVLDIKTSEEYIVAGQRRLMDFQELSDVAELRKLFDAFREKHELLALL
                     DKSMATTGIQIFIGEESGYQMFDNCTVITSPYTTEDGAIGVLGVIGPTRISYQKVIPI
                     VDVTAKLLNQPLNLEN"
     gene            2126..2704
                     /locus_tag="QGH35_00065"
     CDS             2126..2704
                     /locus_tag="QGH35_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HJL80960.1"
                     /translation="MEDDNNSDASVSSEDQKKKQTEKQQESESILTHTEELEKEKQKA
                     SENYDQMLRLQAEIENVRKRTLKDIESASRGSIERVIIELLPVLDSFDLGLSLKTETI
                     EQYKIFKEGQVATLKLITSLLKKLSIEIIDPVEMKYDPELHEVISLQEDHSIQPGYII
                     QVVQKGYRLGDRLLRPARVIVSAQEQENKSST"
     gene            2763..4700
                     /gene="dnaK"
                     /locus_tag="QGH35_00070"
     CDS             2763..4700
                     /gene="dnaK"
                     /locus_tag="QGH35_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017940488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HJL80961.1"
                     /translation="MAKVIGIDLGTTNSCVAVIDGDQAKVIENSEGDRTTPSVVAFSK
                     EEERTLVGQSAKRQAVSNPENTISAIKRLIGRKFDDKVVKKDLDKVSYKIIKAKNEDA
                     WVMANGKETAPQAISAEILRKMKETAEAYLGEKVSEAVITVPAYFNDAQRQATKDAGK
                     IAGLEVKRIINEPTAAALAYGMDKKRGDRKIAVYDLGGGTFDISIIEIADVDGENQFE
                     VLATNGDTFLGGEDFDLRIIDYLMDEFKKQTGMDISKDSQAVQRLKEASEKAKTELSN
                     QAQTDVNLPYLTADSSGPKHLNVTLTRAKLESLVEDLIVQTIEPCKIALKDAGLKIGE
                     VNDVILVGGQTRMPKVQAEVKSFFGVEPRKDVNPDEAVAMGAAIQGGVLSGDVKDVLL
                     LDVTPLSLGIETLGAVMTRLIEKNTTIPTKAEQIFSTAEDNQTAVTIHVLQGERERSQ
                     DNKSLGRFDLTEIPAKPRGLPQIEVVFDIDANGIMNVSAKDKETGKEQKIVIQSSGGL
                     SDDDIEKMVSEAEANAEEDKKFRELVDIKNQGDQLVHATEKTLKDLGEKVETEERGKV
                     ESAIAELTEALKGDDKSTIEAKVKTLSEASVGIAQKAFEEAQSKAADAEESSNGSGEE
                     NVVDAEYEEVNEEEKTEEKTD"
     gene            4784..5908
                     /gene="dnaJ"
                     /locus_tag="QGH35_00075"
     CDS             4784..5908
                     /gene="dnaJ"
                     /locus_tag="QGH35_00075"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019366092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HJL80962.1"
                     /translation="MSKQDYYQTLGIAKNSSDADIKKAYRKLAMKYHPDRNPGNAASE
                     EKFKDAKEAYEVLSDSQKRNAYDQFGHAGVNSQGGMGGGFNPGDSINDIFGDMFGDIF
                     GSRRGARSNSQRGSDLRYELTLDLEQAVFGDNINIKIPSLTSCNSCKGTGAKPGTSAT
                     TCNRCEGRGAVRVQQGFFTLQQTCPECRGTGQTIQSPCLDCNGSGRVSRNRTISLKIP
                     PGVDDNDRIRLSKEGEAGLNGGPSGDLYVDINIREHSIFTREGKNLFCTVPISYSSAV
                     LGGVVQVPTINGAVNLTIPAETQSGKLFRLKAKGIRSHRDRITGDLICKVQVETPINL
                     NSKQKKILKEFEDSIKKSNKNHRPQKSSWKDSVKKFFDRL"
BASE COUNT         2099 a          968 c         1192 g         1665 t
ORIGIN      
        1 atttgattcc tatcttatcg ttaacctata gcagttcgct tatcttgtcc ccaataaagt
       61 tttgtcctaa gaacatcgaa gtagtcataa ttttcagggt gtattaattt gataaaagtt
      121 gaagaagctc taatcgataa tatatcctct gatgacaatt ctgtaaaatc atctccatct
      181 aaatctattg ctgcagtttc atcatcattt aagtcaacag tgacattgat ttcagaatga
      241 gccggaataa ccaaaggtct gtcgtttagc gaatgaggac aaattggaac taaaacaaag
      301 gcctcaatat ccggttttag tattggtcca ccacaactaa gggcataggc tgttgaaccc
      361 gttggtgtgg caacaacaaa gccatctcct tcatgggtat tgatataatt attatctata
      421 gacgttttaa tacgtatcat tcttcctgtc tctattcttt taatggctat atcatttaat
      481 gcatagccag aaaaacaatc tttatctttt ccattgactc tgatttcaat taataatcgc
      541 ttctcctctt tataaacgcc attgataatt ttaatcaatg tttctccagc ttgatcagga
      601 ttaatatcag ttaaaaaccc caatctgcct ctgtttattc cggtgatggg aatcttatat
      661 tgatgggcaa gctttgctga cttcaacatc gttccatcac caccgattga gacaattagg
      721 tcattattcg gtaagtcttc ttttttaatt tcttgtgctt tatttgatat ctcagtagaa
      781 gaagaatctc catccaattt gaagtcataa aatcctatct gcagttcttg atgattcaat
      841 tcatcaagaa tgatattcaa cgcttccatt gagttattat catgggaatt tgctgcgaat
      901 attatgtttt taaaggttgt cattgtattt aggtcttgac tagttattct atattggaaa
      961 tgataacaga atgtaatttt gtagtttcta attcggcaaa ttatttattt gaaataatat
     1021 atcaaggaaa tgaaagaata taatgtagac agtagcccat cacttagggc taaattaata
     1081 ttatcagcta ttgtagacaa atatgtccaa gatggcaccc cagttggatc aaagagttta
     1141 tctctgactg attctatcga tctaagtcca gcaaccattc ggcatgtcat gtctgatctc
     1201 gaagaattgg gatttatcgt ttcaccacac acatcctcag ggagaattcc aacccccaaa
     1261 ggataccgtt tttttatcga tagcctgctt aaattacaac ctgttgaaca aaatgagatt
     1321 caaactatca aagcaagagt tgaattaagt aaaaacagtg gccgggagct ggcaaaaagc
     1381 gtttccagta ctttgtcagc aataacaaaa ctagcaggaa tcgttactat tccaaaacag
     1441 tcagtaacaa gattgaaaga aatggatttt attccattat ctgataagaa aatacttgca
     1501 atcattgtca ctaatgaagc agaggtggag aaccgtattt tagaaatgaa taaggtttat
     1561 tccagagaag aactcaatcg ggcagctaat tatctaaaca ctaactttag gggtcggagt
     1621 ttagtctata ttaaaaaaca attgatcgaa gaattacgaa caacaaaaga gtcggtaaca
     1681 gcgatgatga ccgatttaat aaacattgcc gatcaggttt tagacatcaa aacatctgaa
     1741 gagtatattg ttgctggtca aagaagactg atggatttcc aagaactttc agatgttgca
     1801 gaattacgaa aactttttga tgctttcaga gaaaaacatg aattactggc cctgctggat
     1861 aaaagtatgg ccactactgg tattcaaata tttattggtg aagaatcagg atatcaaatg
     1921 tttgataact gcactgtcat tacctcacct tatactactg aagacggagc cataggggtt
     1981 ttaggtgtga taggcccgac cagaatttct tatcaaaaag ttataccaat tgttgatgtc
     2041 actgcaaaac ttttaaacca accattgaat cttgaaaatt aacccctaga tacttctttg
     2101 ttagaaaaat taattgggta aatcaatgga agatgacaac aactctgatg cttcagtttc
     2161 gtctgaggac cagaaaaaaa agcaaacaga aaaacaacaa gagtcagaat ctatcttgac
     2221 gcatacagaa gagcttgaga aagaaaaaca aaaagcctct gagaattatg atcagatgct
     2281 tcgcctgcaa gcagaaattg agaacgttcg gaaaaggacc ctcaaggaca ttgagagtgc
     2341 tagtaggggt tcaattgaga gagttattat agaattactc cctgtgttag atagttttga
     2401 tttaggcttg agtttgaaaa cggaaaccat tgagcaatat aaaatattca aagaagggca
     2461 ggtggctacc ctgaaactga taacaagttt acttaaaaaa ctttctattg aaattattga
     2521 tcctgttgaa atgaaatatg atccagagtt gcacgaggtc attagtctcc aagaggatca
     2581 ttcgatccag ccaggctata ttattcaggt ggtgcaaaaa ggttatcgtc tcggggatag
     2641 attattaaga ccagccaggg ttattgtttc agctcaagaa caagaaaata aatcttccac
     2701 ttgaaaattt ctcaactacc cttacatttt gttgaggctt aactttattt ggagaaaaaa
     2761 aaatggcaaa agtgattgga attgacttag ggacaactaa ttcttgtgtt gctgttattg
     2821 acggtgacca agctaaagtt attgaaaata gtgaaggtga tagaacaaca ccttcagttg
     2881 ttgctttttc aaaagaagaa gaaagaacat tggtagggca atctgctaaa agacaggcag
     2941 ttagcaatcc tgaaaatact atatctgcta tcaagagatt gattggtaga aagtttgatg
     3001 acaaagtggt gaagaaagat ttagataaag tatcttataa aatcatcaaa gccaaaaatg
     3061 aagatgcttg ggtgatggca aatggtaaag aaacagcacc acaagctatt tctgctgaaa
     3121 ttctcagaaa aatgaaagaa actgctgaag cttatttagg agagaaagtc agtgaggccg
     3181 ttattacagt acctgcatac tttaatgatg ctcaaagaca agctacaaaa gacgctggaa
     3241 agattgcagg ccttgaagtt aaaagaataa ttaatgaacc aacagctgct gctctggctt
     3301 atgggatgga taaaaaaaga ggcgatcgga aaattgcagt ctatgaccta ggtggtggaa
     3361 catttgatat ttctattata gaaattgctg atgttgatgg tgaaaatcaa tttgaagttc
     3421 tggcaacaaa tggagatact tttttaggag gagaagactt tgatcttaga ataattgatt
     3481 acttaatgga tgaatttaaa aagcaaacag gaatggatat ttctaaggac tcacaagcag
     3541 ttcaaagact caaagaagca tcagaaaaag caaaaacaga actatcaaac caagctcaaa
     3601 cagatgttaa tctaccctat ttgactgcag attcatctgg tcctaagcac cttaatgtta
     3661 ccctgacacg tgcaaaactt gaatcactag ttgaggactt aatcgtacaa acaattgagc
     3721 cttgcaagat tgccttaaaa gatgcgggtt taaaaattgg agaggtcaat gatgtgattt
     3781 tggtaggagg gcaaacaaga atgcctaaag ttcaagctga agttaagtct ttttttggtg
     3841 tagaaccaag gaaggatgtt aacccagatg aagctgttgc gatgggtgca gcaatacaag
     3901 gtggagtgtt gtctggtgat gttaaagatg tcttactact tgatgtgact cccttatcat
     3961 taggaattga aactttgggt gcagttatga ctagactaat tgagaaaaat acaactattc
     4021 ctactaaagc cgaacaaata ttctcaactg ctgaggataa tcaaacggca gtgacgattc
     4081 atgttctgca aggagaaaga gagagatccc aggacaataa atcacttgga aggtttgatt
     4141 tgacagaaat tcctgctaaa ccaaggggct tacctcaaat agaagttgtc tttgatattg
     4201 atgccaacgg aataatgaat gtttctgcaa aagataaaga aacaggtaaa gaacaaaaga
     4261 ttgtcattca atcttctgga ggcttatccg atgatgacat agagaagatg gtttcagaag
     4321 ctgaagccaa tgcagaagaa gataaaaaat ttagagaatt ggttgatata aaaaatcaag
     4381 gtgatcaatt ggtacatgca actgagaaaa cacttaaaga tcttggtgaa aaagttgaaa
     4441 cagaagagcg tggtaaagtt gaatcagcaa ttgctgaatt aacagaagca ctgaaaggtg
     4501 atgataaatc aacgatagag gcaaaagtta aaactttaag tgaagcctca gtaggcattg
     4561 ctcagaaagc ttttgaagaa gcacaaagca aagcagctga tgctgaagaa tctagcaatg
     4621 gttctggtga agagaatgtt gttgatgcag agtatgagga ggttaacgag gaagaaaaaa
     4681 ccgaagaaaa gactgactaa tcggtaatta gaatcgttat aacgaaataa actagacgga
     4741 tagattccaa tgcatctatc ctttctaata ttaggcatgc caagtgagta aacaagatta
     4801 ttatcaaact ctaggaatag ctaaaaattc atccgatgct gatataaaga aagcataccg
     4861 caagcttgca atgaagtacc atccagatag aaatcctgga aacgctgcct ctgaagagaa
     4921 attcaaagat gctaaagaag catatgaagt gctctctgac tcacaaaaaa gaaatgcata
     4981 tgatcaattt ggacatgcag gagtcaattc gcaaggagga atggggggag gtttcaatcc
     5041 tggtgattca atcaatgata tttttggaga tatgtttggt gatatcttcg gatcaagaag
     5101 aggagcaaga tcaaatagtc aaagaggatc tgatttacga tatgagctaa cactagattt
     5161 agaacaagct gtttttggag acaatatcaa tattaaaatt ccatcactca catcatgtaa
     5221 ttcatgtaaa ggaaccggag caaaacctgg aacttcagca acaacatgta ataggtgtga
     5281 aggcagaggg gcagtcagag tccaacaagg cttctttaca cttcagcaaa catgtccaga
     5341 atgtagaggt acaggccaaa ccattcaatc accatgccta gactgcaatg gtagtggaag
     5401 agtcagtaga aataggacaa tttccttaaa aatacctcca ggagttgatg acaacgatag
     5461 gattagactc tctaaggaag gtgaggctgg attaaacgga ggaccttcgg gcgacctcta
     5521 tgttgacatt aatataagag agcactctat atttactcgc gaaggcaaga atcttttctg
     5581 tactgttcct ataagttatt cgtctgctgt tctgggaggt gttgttcagg tcccaacaat
     5641 taatggtgct gtcaatttaa ctattcctgc tgaaacacaa tcaggtaaat tatttaggtt
     5701 aaaggcaaaa ggcatacgct cacacagaga cagaataact ggtgacttaa tttgcaaagt
     5761 gcaagttgaa acaccaatta atttaaattc aaaacaaaaa aaaatactca aggagtttga
     5821 agactcaatc aagaaaagta acaagaacca tcgaccacaa aaaagtagct ggaaagatag
     5881 tgttaaaaaa ttcttcgatc gtttgtgatc ttagaatcat aacc
//
LOCUS       DASCTH010000005         2667 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000007261, whole genome shotgun sequence.
ACCESSION   DASCTH010000005 DASCTH010000000
VERSION     DASCTH010000005.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000007261"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="QGH35_00080"
     CDS             complement(<1..407)
                     /locus_tag="QGH35_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL80963.1"
                     /translation="MNASQRYDSLIKWLEDTLNKPIQSIKSASNDASFRRYYRVHYDD
                     HRTCIAVDSPPEYENGAKFVAIAAIFEKMGIRAPRIKAKNLSEGFFVVTDLGNETMLN
                     AILSKKIKTDHLYKNAMNVLLKIQVKGLECQQNL"
     gene            732..>2667
                     /gene="lptD"
                     /locus_tag="QGH35_00085"
     CDS             732..>2667
                     /gene="lptD"
                     /locus_tag="QGH35_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016348.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HJL80964.1"
                     /translation="MGNQIDIQEKIRITSGNSIITADQASYDEQLGILTIPNNISFEN
                     NDLIIFGKSAEIKIKEKTTIINNTNYQIFSIPARGTAGEIRVKNNNINLSQVTFSTCT
                     SIDDSWELSAQSITIDNLAGIGEARNLVFNFKDIPILFLPYLSFPTGDQRKSGLLTPS
                     FGKSSKKGIEISLPYYWNIASNIDLKTTPTLMTKRGMMLSSELRYMTAHQNGVTQIDY
                     LPNDDKTKKSRTYISHNQVLHLSSNWRMNLNGEYVSDNNYFEDLTGTMSSTSRTHLLR
                     QMQLQHLTDDWFMEIGMDHYQILDESIISKEKPFRRLPYLNFSGAWYNQALSLDYKLN
                     SGVVFFDKSETISGSRFNVMPEITSSLSFNGISIEPSIALDFTKYNLSIPTELRHLKN
                     DTSPERIVPIYSLDMKGIFRKYWNNGKYTQTLEPRIQAVYIPYRNQDDVPIFDTTIPD
                     FRVQLFNKNRYVGQDRLGDTSQMSYGITSQLFNTSDGESILGFTFGQIRYYKDRKVSL
                     PHETSLTNNSSSYLASIDWKINKEWSMKLGNIWDSHKNQSMKTVAKFQYVSEESKIFN
                     LSYRYRKNILKQMDVSFSWPVRDQWNVVGRYNYSILDRRVLEHFVGLEYESCCWGIRL
                     ITRKHLAYRNGETDSSVSLEF"
BASE COUNT          927 a          473 c          450 g          817 t
ORIGIN      
        1 agattttgtt gacattccaa tcccttcacc tggatcttca acaggacatt catggcattt
       61 ttgtacaaat gatccgtttt gatttttttt gaaagaatgg catttaacat ggtttcatta
      121 cctagatcag tcactacaaa aaaaccctca gaaagatttt ttgccttaat tcttggtgct
      181 ctaatgccca tcttctcgaa aattgcagca atggcaacaa atttagcgcc attttcatat
      241 tcaggaggcg aatctactgc tatgcaagtt ctatgatcat cgtagtggac tctataataa
      301 cgtctaaaac tagcgtcatt gcttgctgat tttatggatt gaataggttt atttagagta
      361 tcttctaacc acttaattaa tgaatcgtat cgttgagatg cgttcaaaaa aaccaccttt
      421 acaaaaatta ttttaactta gaaagtaatt catattacat caaagttttt aactaataac
      481 ctgaaagaat aggaaaataa cacaggaaaa ttataaaatt agcataatct aatatatgga
      541 agatactttt gcgtttattt ttaactatta gtctagtaat agttgtattg ggagtcacgc
      601 tcaatgccta agctattggg cccggtttac attcatccgt ttgtctggcc aatacgatag
      661 caataaattc agaaactcct agtgacagta gtcagagtct ttcatttgaa actggttcga
      721 ttcaggccga attgggaaat caaattgata ttcaagaaaa aattaggatc acttctggta
      781 acagtatcat tactgcagat caagcatcct acgacgaaca acttggcatc ctgacgatcc
      841 caaacaatat atcttttgaa aataatgatt taatcatttt tgggaaaagt gcggaaatca
      901 aaataaaaga aaaaactact atcatcaaca acacaaacta tcaaatcttc tcaattccag
      961 ccagaggtac agctggagag ataagagtga aaaataataa cattaatctg agtcaagtta
     1021 ctttcagtac atgcacatcc attgatgatt catgggaact atctgctcaa tctataacaa
     1081 tcgataacct agcaggcata ggtgaagcca gaaatcttgt ctttaatttc aaagatattc
     1141 cgattctatt tttgccttat ctatccttcc caacaggtga tcaaagaaaa tctggactgc
     1201 tgacaccaag ctttggtaaa tcttcaaaaa aaggtattga aattagtttg ccgtactatt
     1261 ggaatattgc ttcaaatatt gatcttaaaa caactccaac acttatgacg aaaagaggaa
     1321 tgatgctttc atcagaatta agatatatga cagcacatca aaatggagtg acccaaatag
     1381 actaccttcc aaacgatgac aaaacaaaaa aaagtcgtac ctatatatca cataatcaag
     1441 tgcttcactt aagttctaat tggcgaatga acctgaacgg agaatatgta tcggacaata
     1501 attattttga agacttaacc ggaacgatga gctcaaccag tcgaactcac ttgttaagac
     1561 aaatgcagtt acagcatctt actgatgatt ggttcatgga aataggcatg gatcactatc
     1621 aaattttgga tgaatcaatt atttctaaag aaaagccttt taggcgttta ccatatttga
     1681 atttctctgg agcttggtat aaccaagcat tgagcctaga ttataagtta aatagtgggg
     1741 tagttttttt tgacaaatca gagaccattt ccgggtcaag atttaatgta atgcctgaaa
     1801 taacctcttc attatctttt aatggcatca gcatcgaacc ttctatagct ctagacttta
     1861 caaaatacaa tttaagtatt ccgacagaac ttcgtcattt aaaaaatgat acctcccctg
     1921 aaagaatagt cccaatatat agtttagata tgaaaggaat atttcgtaaa tattggaaca
     1981 atggaaagta tacacagact cttgaaccta ggattcaagc agtttatatt ccgtacagaa
     2041 atcaagacga tgttccaatt tttgacacca ctattccgga ttttagagtt caattattta
     2101 acaaaaatag gtatgttggc caggataggt taggtgatac ttcccaaatg agttatggaa
     2161 tcacaagtca actgtttaat accagtgatg gagaatcgat actaggtttc accttcggtc
     2221 aaattcgcta ttacaaagat aggaaagtgt cgcttccaca tgaaacatct ttgaccaaca
     2281 attcctcaag ttatctggca tcaatagact ggaaaattaa taaagaatgg tctatgaaac
     2341 ttggcaatat atgggattca cataaaaatc aatcgatgaa aacagtagca aaatttcaat
     2401 acgtatctga agagtccaaa atcttcaatt tatcttatag ataccgcaaa aatattctca
     2461 aacagatgga tgtctctttt tcatggcctg tacgagatca atggaatgta gtcggacggt
     2521 acaattactc catccttgat agaagagtct tagagcactt tgtcggcttg gaatatgaat
     2581 catgttgttg gggcattcga ttgatcacta gaaaacacct tgcttataga aatggggaaa
     2641 ccgactcttc ggtatcgtta gaattta
//
LOCUS       DASCTH010000006         2574 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000009277, whole genome shotgun sequence.
ACCESSION   DASCTH010000006 DASCTH010000000
VERSION     DASCTH010000006.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000009277"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="QGH35_00090"
     CDS             <1..915
                     /locus_tag="QGH35_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012993.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HJL80965.1"
                     /translation="PKPGIKSNLDHMLFLIDAAQGYGGHKDYLAFHEFPITGWDQWSR
                     EEILGFALRLPGPETEAIGKKAIEHNCYISFGTYAQYKDWPRHIMSVSVIINPKGEII
                     SEQWKARNIHGVFVGFELFTTTVYDVLDRYVEMYGWDAVLPVAKTDIGNITITPCVME
                     PEIFRGMAMKGAELIIKTASGGSNRIDAAAVAQANKVYTGLVNNSVSPNNRSFIEDAG
                     GGGTSIYEPWGKCVAEAETHHEEIVTHTIPMQSFRDGHKTPDIHYELITHLTDQYVSR
                     YGPNGFLKYLPEGLKEAGEYFLKKSRWK"
     gene            1142..2143
                     /locus_tag="QGH35_00095"
     CDS             1142..2143
                     /locus_tag="QGH35_00095"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HJL80966.1"
                     /translation="MKNFITTQDWSKQDLQEILDYAKNLKENKFQDLLKNKSVALLFF
                     NPSMRTKVSFELGIQELGGYAVVLHPGSDAWPIEFEPAVIMNENPEEHIKEVARVLSN
                     YCDLIAVRAFPKFINLEEDFEDRVINSFAQYATVPIVNMETITHPCQELAHILTLQEN
                     LGNLRGKNYLLTWTYHPKPLNTAVANSSILIASKFGMNVKLLCPSPNYNLHEKYLEQA
                     KKNCEENETKFSITHDIDQAYSDSDIVYAKSWGSLLHYGDVEKELIQRHKNKHFIVDE
                     EKMAKTNNAIFSHCLPLRRNVKATDGVMDADYCVAIQEASNRKHVQKSLLTKLLKDK"
     gene            2145..>2574
                     /locus_tag="QGH35_00100"
     CDS             2145..>2574
                     /locus_tag="QGH35_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HJL80967.1"
                     /translation="MKKIVLAFSGGLDTSFCIPFLIEKGFEVHTVFVNTGGTSPREET
                     KITRRANELGATKHVNVNVEQSFWSQIIKPLIWSGSSYQNKYPALCSDRYLIVKEAVK
                     LCKTLKTKNIAHGCTGMGNDQVRFDLSIQALGIFNIITPIR"
BASE COUNT          896 a          458 c          513 g          707 t
ORIGIN      
        1 ccaaaacctg gaattaaatc aaacctggat cacatgttat ttcttattga tgctgcccaa
       61 ggctacggcg gccataaaga ttacttagcc tttcatgaat ttccaattac aggttgggac
      121 caatggagta gggaagagat actgggattt gctctgaggc ttcctggacc tgagactgaa
      181 gccattggca aaaaagccat agagcataat tgctatatta gtttcggcac ctatgcacaa
      241 tacaaagact ggccacgtca tatcatgagt gtatctgtga tcatcaaccc taagggtgaa
      301 atcatatctg aacaatggaa agctagaaat attcatgggg tctttgtggg attcgagctg
      361 ttcacaacaa cagtgtatga cgtcctagat cgctatgttg aaatgtacgg ctgggatgca
      421 gtgttgccag ttgccaaaac tgatattggc aatatcacca tcacgccctg tgtcatggaa
      481 cccgaaatct tcagaggcat ggccatgaaa ggagcagaac ttatcattaa aacagcgtcg
      541 ggtggctcca atagaattga tgctgcagca gtggcccaag caaacaaagt ctacacaggc
      601 ctagtgaata attccgtgtc tcctaacaac agaagcttca tagaagatgc aggaggaggt
      661 ggaacctcaa tctatgagcc ttggggcaaa tgtgtagctg aagcagaaac tcatcacgaa
      721 gaaattgtaa ctcatacaat accaatgcaa agctttagag atggacacaa aacacctgat
      781 atccattatg agttaattac ccatttaaca gatcagtacg tttctagata cggacctaat
      841 ggatttttga aatatctccc tgaaggactc aaagaagcag gcgaatactt tttaaaaaaa
      901 tcgcggtgga agtaagaaag atattaaaaa atactaccag acgaatcaat aagaaatata
      961 tattcatcaa aataagataa aacctttaca aactgatagc gatatgaata atgacactat
     1021 gtttaattta attagagaag aaggtaacag gagaccggca ttctagctgg cgtctgcttt
     1081 tttctaaaat aattaataga cccagcataa tagctgggtt tttttttatc tgaatgaaaa
     1141 tatgaaaaac tttattacaa ctcaagattg gtccaagcag gatcttcaag aaattttaga
     1201 ctatgcaaaa aacttaaagg aaaataagtt tcaagatcta ttaaagaata aatctgtggc
     1261 cttattgttc tttaatccct ccatgagaac taaggttagt tttgaattag gtattcagga
     1321 gcttgggggc tacgcagttg ttttgcatcc aggtagtgat gcatggccca tagaatttga
     1381 gcctgcagtc attatgaatg aaaaccctga agagcatatc aaagaagtcg caagagtgtt
     1441 atccaactat tgtgacctta tagcggtaag agccttcccg aaatttataa atctagagga
     1501 ggattttgaa gacagagtga tcaatagttt tgctcagtat gcaactgtcc ctattgttaa
     1561 tatggagacc atcacacacc cttgtcaaga attggcacat atactcactc tccaagagaa
     1621 cttgggcaac ttaagaggta aaaactacct ccttacatgg acttatcatc caaaaccgct
     1681 taataccgcc gttgcgaatt caagcatact aattgcgagt aagtttggaa tgaatgtaaa
     1741 acttctatgt ccatcgccaa actataactt gcatgagaaa tatcttgagc aagctaaaaa
     1801 aaattgtgaa gaaaatgaga ccaagttctc tatcactcat gatattgatc aggcctattc
     1861 agattcagac atagtttatg caaaaagttg gggatcctta ttgcactatg gtgacgtaga
     1921 gaaagaattg atacaaaggc ataaaaataa acattttatt gttgacgaag aaaaaatggc
     1981 aaaaacaaat aatgcgattt ttagccactg tttgccatta agaagaaatg ttaaagctac
     2041 tgatggtgtg atggatgccg attattgtgt agcaattcag gaagctagca atagaaaaca
     2101 tgtacaaaaa tcattactaa ctaaattact taaggataaa taagatgaaa aaaattgttt
     2161 tagcattttc aggtggtcta gatactagtt tctgcattcc tttcctaatc gaaaaaggtt
     2221 ttgaggttca tactgttttt gtgaatacag gcggcacttc tcctagagag gaaaccaaaa
     2281 ttacccgcag agcaaatgaa ttaggggcta caaagcacgt aaatgtgaat gtagagcaat
     2341 cattttggtc tcaaataatt aaaccattga tttggtcagg ttcctcttat cagaataagt
     2401 atcccgccct atgctcagat agatatttga ttgtcaaaga agccgtaaaa ctatgtaaga
     2461 ctctaaaaac aaaaaatatt gcacatggat gtactgggat gggaaatgat caagtcagat
     2521 ttgatttatc aattcaggcg ctaggcatat ttaacatcat tacacccatc agag
//
LOCUS       DASCTH010000007        10174 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000013189, whole genome shotgun sequence.
ACCESSION   DASCTH010000007 DASCTH010000000
VERSION     DASCTH010000007.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000013189"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /gene="bamA"
                     /locus_tag="QGH35_00105"
     CDS             <1..1304
                     /gene="bamA"
                     /locus_tag="QGH35_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HJL80968.1"
                     /translation="EVLRRELRQLEGAYMSKSRLERSKVRLQRLPYIEEVEFDTIPVA
                     GTNDLVDAEFTIKEGLPGQFGGGIGYSGAQKMILNGNFVHTNFFGTGNRVALDLNASK
                     YRDLYSFSHTNPYTGLNEISRTVRFSYSTFNQFTSSASDFSTKTWSTGLEYSYPLSEY
                     QRVIFGSTLQSSELLAGTYSANQALQWVSNNGDNNCFDEEYFNFCKTKFNAAELLLGW
                     VYDSRNRFLFADRGMSQRLIISSAIPGSDVEYYGINFSYKQYFNIPFPWVNYLTLMAK
                     ADIAFNDAYGDTIGVPPYKNFFGGGPDSVRGYKEHHLGPRDNFGNPYGGNLLMSAQLE
                     VILPMPQKWASRARFSMFFDIGNVFSTTEIDFFDKMGDPVDYDFDAGNLKQSFGLAAQ
                     WLAPLGLFRFSYAMPLNDDSPTSTSWGDETERFQFTIGGAF"
     gene            1336..1899
                     /locus_tag="QGH35_00110"
     CDS             1336..1899
                     /locus_tag="QGH35_00110"
                     /inference="COORDINATES: protein motif:HMM:NF015871.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HJL80969.1"
                     /translation="MKKTNFVLLIIIGAVFLTQPIFAQTAVEARIGVVDMNKLLQESP
                     QFQDMQEAIEEEFAPRVRDISSKESAFNASRDQLTKDNLALSTDERQSATLKLASDQR
                     ELEYLAQSFQQDRNSRSQIELNKIFREIIDIIKKFGRDNGYDLVINEGKIDQNTLLSG
                     GTLYTGASVDITNDVAKLLEKKFQEDK"
     gene            1955..2986
                     /gene="lpxD"
                     /locus_tag="QGH35_00115"
     CDS             1955..2986
                     /gene="lpxD"
                     /locus_tag="QGH35_00115"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HJL80970.1"
                     /translation="MSETVITLGQLASQYDCKIHGDSSVVIADVATLSSATNSSLTFL
                     ANSKYKSVLKETKAAAVVLTQNDLKFCPTNALVTNEPYLLFAQIASHFDTTKEFNPEI
                     HTSATIHPSSQVSSTCGIESGVVIAKNVVLGEHVFVGANTVIGQDTKVGQSSWISSGV
                     MIGAKTKIGSRTIIHSGVVIGADGFGFSENKDSEWVKIPQMGCVNIGNDVEIGANTTI
                     DKGTFTDTILGNGVKLDNLIQIGHNVIIGDHTAIVAQTGIAGSATIGKRCKIGGQVGI
                     VGHLKIADDTTITARTSVTKDIKDSGVYSGNLFSTQKATEWQKNAASFKKLNKLQKLV
                     KAISKKIGD"
     gene            2989..3759
                     /gene="lpxA"
                     /locus_tag="QGH35_00120"
     CDS             2989..3759
                     /gene="lpxA"
                     /locus_tag="QGH35_00120"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HJL80971.1"
                     /translation="MIHETVVISKEAQLDDSVTIGPYSIIGPDVSIASGTIIESHVVI
                     TGRTKIGQDNHIYSFSSIGDHPQDMKYKGEDTELEIGDRNTIREYCTINKGTIQDTGV
                     TRLGNDNWIMAYVHLAHDCQVGSNTIFANNTTLAGHVEVGDYAILGGFSGAHQYCKIG
                     CHSFLGMYSGVSRDLPPYVIVTGQPAIPRGINLEGLKRRDFNPDQIRNIKTAYKYLYK
                     SELRLEEAQEKIAGLNEVSNELQVMINFLKASSRGIVR"
     gene            3781..4923
                     /gene="lpxB"
                     /locus_tag="QGH35_00125"
     CDS             3781..4923
                     /gene="lpxB"
                     /locus_tag="QGH35_00125"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HJL80972.1"
                     /translation="MSRFVIVAGESSGDLLASKIIDSIKEQMPDATFEGVAGPQMVKA
                     GCLDWFNSSELSVMGIFGVLKHLPRIFLIRRRIIKRILNNPPDAFIGIDAPDFNLALE
                     KKLKNKGIKTIHVVSPSFWAWRKNRVRSLNQSTDLLLCLFPFEEDLLRQHNVNSIFIG
                     HPLADSVDHGIDILSARNALQLDAQAKTVITLMPGSRNTEIKRHAGLLFEAADLISKK
                     YDLVEFVVPVVNYTIEQELKKLLTDRFQNLNVVITDNATLALSACDLVITKSGTSTLE
                     AALHKKPLIVVYKMSSLSYWFLRIFKIVKTNYISLPNILLGKEVVPELIQKEAHPESI
                     FNASVLYLSSQKVVDELQEQFDGLYLELKRNASMLAANAIIEIIEE"
     gene            4959..5531
                     /locus_tag="QGH35_00130"
     CDS             4959..5531
                     /locus_tag="QGH35_00130"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HJL80973.1"
                     /translation="MKLIAGVDEAGRGCLAGPVIAAAVIIPNNQKIEGLKDSKKLSSI
                     RRQQLFTIIKTQAIEWSVGLANHQEVDQYNVLRATMLAMQRAIEKLQNKGIKVLIDGP
                     HCPMIDSNKNIMMEPKIGGDDRYPCISAASVIAKVTRDQFMLKMHKKFPQYHFDKNKG
                     YPTKDHLSAIQEFGPTSIHRMTFKSEIYNS"
     gene            5533..9000
                     /gene="dnaE"
                     /locus_tag="QGH35_00135"
     CDS             5533..9000
                     /gene="dnaE"
                     /locus_tag="QGH35_00135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HJL80974.1"
                     /translation="MIKKEPLFIHLDVFSEYSIGQSIIRIESVLDKCFEDHMPALGIV
                     EHNNLFSAFKFYKKAVHLGIKPIIGANISVQTDRSNVICNMTFLCKNSTGYKNLSHLI
                     TRSYIEGYRNDQASIDIDWLNEKNSSGLIAISGGHKGLIQALLLSGKTNDAADQLKQL
                     QAIFFNDVYLSIQRLGNNRDEELLHKTLECSLSSNTPLLAANNPRFLNKGDYLALDAR
                     VCIDQSDVIDNVKRIKDYTNEQYFKTQEEMSNLFSDISEALDNTVNIAKKCNFKFTTD
                     QVALPAYLPPKGLSVEGYLKREAKQGLDQLVDENEKLTKIYNQRLNTELDIIISTGFS
                     GYFLIVSDFVRWSRVKNIPVGPGRGSGPGSLVAFCLGITDIDPIRYDLLFERFLNPER
                     VSMPDFDIDFCVKGRDQVIDYVSDQYGSEMVSQIITFGTMSAKAVVRDVGRILGYPFG
                     MVDQIAKLIPFEIGITIENALKKESELNERYENEEEVQAIINLALQLEGLVRNAGKHA
                     GGVVIAPSNLSDFMPLYTVEGDEGGTVTQFDKDDVESMGLIKFDFLGLKTLTVIQTAV
                     ELIHQQQSDEQLKTIDIRKIPTDDIHTYQLLSSAKTVGVFQLESIGMRDLIKRMQPEK
                     FDDIIALVALFRPGPLLSGMVDDFIERRKADQSRLIDYLHPMLESILRTTYGVIVYQE
                     QVMQIAQKLAKYTQGSADILRKAMGKKIPEEMAKQKDVFIQGAIDNDVKERTAIKIFD
                     LIEKFAGYGFNKSHSVSYAFIAYQTAWLKTHYPAQFMAASMTADINNTDKIIPLKEDC
                     TNLDIEILPPCINHSAYDFFVQDSESIRYGLGAIKGIGESAAEAMTSERLTNGTYKDF
                     FDFCSRLTDQKLSKRTIEALVKSGAMDCLNENRSTLFRSIGTGVNFANKKAMDQKSGQ
                     NNLFSIDGDHAETPPSLNRDKEWKLNDLLQGEFQSLGFYFSGHPFDPYRKDCIHLTKG
                     PLIQIKAKMEKSNNGSLLFNSQNFMEIAGLVFDVKRRGNNLIFRLDDGTAIIDAIVFG
                     ENRDRFRDLINNNAILHLNGSFRYDTYADTWQFVASEILELDALIEKKAKTLLIKCNP
                     DFDPEKLKSILQAHTPGSCKVNVHYKTDIDVYKLKFGQEWTVKVTKELRDRLTAEFGS
                     GNFQFLTYH"
     gene            9082..10041
                     /locus_tag="QGH35_00140"
     CDS             9082..10041
                     /locus_tag="QGH35_00140"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HJL80975.1"
                     /translation="MDFLNFEQPIVELERRIEELKLSVTDSEYSEDIDKEINKLSQKC
                     EALTNKIFSNLTEWQIVQLSRHPNRPKSSDVIKAITPDFQELHGDRMYADDTAIISGL
                     GTIDNLSMMLIAQEKGKDTKSRVYRNYGMPRPEGYRKALRVMKLAEKFSIPIVTIIDT
                     PGAYPGIGAEERGQSEAIAKNLFTLASIRTPVINLVLGEGGSGGALAIGMGDSLMMLQ
                     YSVYTVISPEACSSILWRNPEETEIAADSMGLSAKRLSKLGVVDQVIKEPLGGFHRDP
                     EPMYQQITKAIVDEVSLLSNMNMSDLLERRRVKFRAIGQFKEI"
BASE COUNT         3370 a         1610 c         2077 g         3117 t
ORIGIN      
        1 acgaagtttt gcgacgtgaa ctaaggcagt tggaaggtgc ttatatgtcg aaaagtagat
       61 tggaacgctc aaaagttaga ttgcaaagat tgccctatat agaggaagtg gaatttgaca
      121 caattccagt tgcaggaacc aatgatttag ttgatgcgga gttcacgatt aaagagggtc
      181 ttccaggaca atttggcgga ggcataggat attctggtgc acagaaaatg attttaaatg
      241 gcaatttcgt tcacaccaat ttttttggta ccggtaatag agttgcactt gatttaaacg
      301 caagtaaata cagggatctc tacagttttt ctcatacgaa tccttatacc ggattaaatg
      361 aaatttccag aacggtaaga ttttcatatt caaccttcaa tcagttcacc tcgtctgctt
      421 ctgatttttc aaccaaaact tggtctacag gattggaata ttcataccct ttaagtgaat
      481 accaacgagt tattttcggt tcaaccttac agagttccga attgttagct ggaacttata
      541 gtgcaaatca agcacttcag tgggttagca acaatggcga taataattgt tttgatgaag
      601 aatattttaa tttttgtaaa accaaattta atgctgcaga actcttactg ggttgggttt
      661 atgattccag gaatcgtttt ctttttgctg accgaggcat gtcgcaaaga ctcataatca
      721 gctctgcaat acctggcagt gatgttgagt attatggtat taacttcagt tataaacagt
      781 attttaatat accgtttcca tgggtgaatt atcttacatt gatggcaaaa gctgatattg
      841 cattcaatga tgcatatggt gatactattg gcgttcctcc atataagaac tttttcggag
      901 gaggtccaga ttctgtaaga ggctataaag aacatcatct tggaccaagg gataattttg
      961 ggaacccgta cggtggaaat ttattgatgt cagcccaatt ggaggtcatt ttgccgatgc
     1021 cacaaaaatg ggcttcaaga gcaagattta gtatgttttt tgatattggt aatgtctttt
     1081 ccaccactga gatagatttt tttgataaaa tgggtgatcc tgtcgattat gattttgatg
     1141 caggcaactt aaagcaatct tttgggttgg cggcacaatg gttggctcct cttgggttat
     1201 ttaggtttag ttatgcgatg ccattaaatg atgattctcc aacttcgact tcatggggcg
     1261 atgaaactga acgattccaa tttacaattg ggggagcttt ttagacaata gactattatt
     1321 ttatagagga tagatatgaa gaaaacaaat tttgtattat tgataattat tggagcagtg
     1381 tttttaacac aacccatttt tgcacagact gcagtcgaag ccagaattgg tgtggttgat
     1441 atgaataaat tgcttcaaga gtcacctcag ttccaagata tgcaagaggc cattgaagaa
     1501 gaatttgctc ccagggtaag ggacatttcg tcaaaagaat ctgcatttaa tgccagtaga
     1561 gatcagttga ctaaagataa tcttgctttg agtacagatg agcgccagag tgctacctta
     1621 aaacttgctt ctgaccaaag ggagcttgaa tatttagcac agagctttca acaagatcgc
     1681 aatagtagaa gtcaaattga attaaataaa atttttagag aaatcataga tattattaaa
     1741 aaatttggca gagataacgg ttatgatcta gttatcaatg aaggcaagat cgatcagaac
     1801 accttattga gtggcgggac attgtacaca ggcgcctctg ttgatatcac caacgatgta
     1861 gcaaaacttt tagagaagaa gtttcaggaa gataaataaa attggaggct agttaaaagg
     1921 cgtctagttt tgcaaatgtt ggagagttag aattttgtct gaaactgtga ttactctagg
     1981 gcaattagct tctcagtacg attgtaaaat tcatggagac tcctctgtgg ttatagccga
     2041 tgtagcaact ctgagtagtg ctacgaatag ttccctaaca ttcttagcaa actctaaata
     2101 taaatctgtt ttaaaggaaa caaaagcagc agcagttgtg ctgactcaaa atgatcttaa
     2161 gttttgccca actaatgctt tggttacaaa tgagccatat ttattatttg ctcaaattgc
     2221 gagtcatttt gataccacta aggagttcaa tccggagatt catacttcag cgactattca
     2281 tccatccagt caagtttcct ccacttgcgg gattgaatcc ggtgtagtta ttgctaagaa
     2341 tgttgtcttg ggagagcatg tttttgtggg ggccaataca gtaataggac aggataccaa
     2401 ggtgggtcaa tcttcttgga ttagttcagg agttatgatt ggagccaaaa caaagatagg
     2461 ttcaagaact attattcatt caggagttgt cattggggcg gatggtttcg ggttttcaga
     2521 aaataaagat tctgagtggg taaaaattcc tcagatgggc tgcgttaata ttggcaatga
     2581 cgttgaaatt ggtgcgaata caaccatcga caaaggaacc tttacagaca ccatcctcgg
     2641 aaatggggtg aaattagata atctcataca aataggacac aacgttatca ttggtgacca
     2701 cactgctatt gttgctcaaa caggaatagc agggagtgct acaattggta agcgttgtaa
     2761 aattggtggt caagttggga ttgttgggca tctgaaaata gccgatgata ctaccattac
     2821 agcaagaaca tctgtcacta aagatatcaa agattctgga gtctattctg gcaatctgtt
     2881 ctcgacccaa aaagcaacag aatggcaaaa aaatgcagcc agttttaaaa aattaaacaa
     2941 gcttcagaaa ttagtaaaag ccatcagtaa aaaaataggc gattaaaagt gattcacgaa
     3001 actgtagtga tttctaaaga ggctcagtta gacgatagtg taaccattgg tccttattca
     3061 atcattggac cagatgtttc gattgctagt ggcacaatta tagaatccca tgtagttatt
     3121 actggcagaa ccaaaatagg acaagacaat catatctatt cattttcttc aattggagac
     3181 caccctcaag atatgaaata caaaggtgag gatactgaat tagaaatagg agatagaaat
     3241 actatcagag agtattgcac tatcaataaa ggcactattc aagacacagg tgtaacgcgg
     3301 ttgggaaatg ataattggat tatggcctat gtccatctag cccatgactg ccaagttggt
     3361 agtaacacta tcttcgcaaa caacacgacc ctggctggtc atgtggaagt gggtgactac
     3421 gcgattttag gtggtttttc aggtgctcat caatactgta aaataggctg ccatagtttt
     3481 ttgggaatgt actctggggt gtcgagagat ttgccccctt atgtcattgt tacaggacaa
     3541 cctgccatac ctaggggaat taacttagaa ggtctaaagc gaagagattt taaccccgac
     3601 cagatcagaa atataaaaac tgcttacaaa tatttatata agtctgagtt aaggttagaa
     3661 gaagctcaag aaaaaattgc tggtcttaat gaagtttcaa atgaactaca ggtaatgatt
     3721 aattttctga aagcttcttc aagaggaatt gttcgttgaa ttctataaaa tccagttatt
     3781 gtgtctagat ttgttattgt agcaggagaa tcatcaggag atttattagc ttccaagatt
     3841 attgattcaa taaaggagca aatgccagat gctacatttg agggtgtagc cggtccacaa
     3901 atggttaaag ctgggtgctt ggattggttt aattcctcag agctttctgt gatgggtata
     3961 tttggtgttc ttaagcactt accaagaatt tttttaatca gaagaagaat aattaaaaga
     4021 attctcaata atcctcctga tgcatttatt ggaattgatg ctcccgattt taatttagca
     4081 ctcgagaaaa aactaaaaaa taaaggtatc aaaactatcc atgtggtatc gcccagtttt
     4141 tgggcatggc gcaaaaatag agttcgatcc ctaaatcaat ctaccgattt attactctgt
     4201 ctttttcctt ttgaagaaga tcttttaagg cagcataatg tcaattctat ctttattgga
     4261 catccattgg ctgattcagt agatcacggt attgatattt tgtctgcgag aaatgctttg
     4321 caactggatg ctcaagcaaa aactgtaatc acactgatgc ccggtagtag aaatactgaa
     4381 attaaaagac atgcaggatt attgtttgag gcagctgatt tgatttcgaa aaaatatgat
     4441 cttgttgaat ttgtggtccc agtagtaaat tacaccattg aacaagaact taagaagtta
     4501 ctaaccgatc ggtttcagaa cctcaatgta gttattactg ataatgcaac attagcctta
     4561 tcagcgtgtg atttagtcat tacaaaatct ggcacttcta ctcttgaagc agccttgcat
     4621 aaaaaacctt tgattgtggt atataaaatg tcttctctga gctactggtt tttgagaatt
     4681 ttcaagattg ttaagacaaa ttatatttca ttacccaata tccttcttgg aaaagaagta
     4741 gtgcctgaat tgatccagaa agaggcacat cctgaaagta tattcaatgc cagtgtgttg
     4801 tatctttcca gtcaaaaagt ggtcgacgag cttcaagaac aatttgatgg tctctacctt
     4861 gagttgaaaa gaaatgcttc aatgctcgca gcaaacgcaa tcatcgagat tatagaagaa
     4921 taataatggt ttgattgtct aatttatgtt ttgattctat gaagttaatc gcaggtgttg
     4981 atgaggcagg aagagggtgt ttggccggac cggttattgc tgccgctgtg atcattccta
     5041 acaatcaaaa gatagaaggt ttgaaagatt caaaaaaatt gtcttccatt agaagacaac
     5101 aattatttac catcataaaa actcaagcaa tagagtggtc cgtcggtctt gctaatcatc
     5161 aggaagtgga tcaatataat gttctgaggg caacaatgct cgcaatgcag agggcaattg
     5221 agaaactaca aaacaagggt attaaagtct tgatagatgg cccgcattgc ccaatgatag
     5281 attcaaataa gaatataatg atggagccaa aaattggtgg agatgatcgt tatccttgta
     5341 tttcagcagc atcggtaata gcaaaagtaa ctagagatca atttatgttg aaaatgcata
     5401 agaaatttcc tcaatatcat tttgataaaa acaaaggtta tcctactaag gaccatttat
     5461 cagctattca agaatttggc ccaacatcaa ttcatagaat gacatttaaa tcagaaatat
     5521 ataatagtta aaatgataaa aaaagaacca ttattcattc acttagacgt tttttctgaa
     5581 tattctatag gtcaaagtat tattcgaatt gaatccgtat tagacaaatg ttttgaagat
     5641 catatgccag cattgggcat tgttgagcat aataatttat tttcagcatt taaattctat
     5701 aaaaaagcag ttcatttggg tattaagcct attattggtg caaatatttc agtgcaaaca
     5761 gatcgttcca atgtgatttg caatatgacc tttctttgta agaattcaac tgggtataaa
     5821 aatttatctc atttaattac aagatcttat attgaaggat acaggaatga ccaagcatcg
     5881 atagatattg attggttgaa tgagaaaaat agttcaggat tgatagcaat ttcaggaggt
     5941 cacaaaggtt tgatccaggc gctcctatta tctggtaaaa caaatgatgc tgccgatcaa
     6001 ctgaagcagt tacaagctat ttttttcaat gatgtttatc tatcaattca gagattaggt
     6061 aacaataggg atgaggagtt attgcataaa actcttgaat gttccttgtc atcaaatact
     6121 ccacttctgg cagcaaacaa tccaagattt ttgaataagg gtgattactt ggctctggat
     6181 gcaagagttt gcatagatca gagtgatgtc attgataatg taaaaaggat aaaagattac
     6241 acaaatgaac aatacttcaa aacgcaagag gaaatgtcta atttattttc agatatttca
     6301 gaagctttag acaacacagt taatattgca aaaaaatgta actttaaatt tacaaccgat
     6361 caagttgccc ttcctgcata tttgcctcct aaaggcttga gtgtagaagg atatttaaaa
     6421 agggaggcga aacaaggctt agatcagctt gttgatgaaa atgaaaagct aacaaaaata
     6481 tataatcagc gcttaaatac tgagctagat attattattt ctaccgggtt ttctggctat
     6541 ttcttaattg tttctgattt tgttagatgg tccagagtaa agaatattcc tgttggaccg
     6601 ggaaggggtt ctgggcccgg atctttggtt gctttttgct tggggataac tgacatcgat
     6661 ccgattcgat acgatttact ttttgaacga tttcttaatc ctgagagagt ttctatgcca
     6721 gattttgata tcgatttttg tgtaaaaggt agagatcaag ttattgatta tgtttcggat
     6781 caatacggtt cagaaatggt ttcacaaatc attacttttg gcacaatgtc agcaaaagcg
     6841 gttgtcaggg atgttggaag aatactgggc tatccttttg ggatggttga tcaaattgcc
     6901 aaattaattc catttgagat tggtattact attgaaaacg cattgaagaa agaatcagag
     6961 ctcaatgagc gatatgaaaa tgaggaggaa gttcaagcaa taataaattt agctctacag
     7021 ttagaaggct tggtaagaaa tgccggaaaa catgcaggtg gagttgttat agctccctcc
     7081 aacctttcag attttatgcc actttatacg gttgagggag acgaaggggg aactgttact
     7141 caatttgata aagatgatgt tgaatcaatg ggactcatta agtttgattt tttaggtctt
     7201 aaaacactta ccgtcataca aactgccgtt gaattaatac atcaacagca aagtgatgaa
     7261 caacttaaaa ccatcgatat tagaaaaata ccaactgatg atattcatac ttaccagtta
     7321 ttatcatcgg ctaagactgt tggagtattc caattggaat caataggtat gagggatttg
     7381 attaagagaa tgcagcctga aaaatttgat gatattatcg ctttggttgc acttttcaga
     7441 cccggtccac tactatcagg aatggtggat gacttcattg aacgaagaaa ggctgatcaa
     7501 tcaagactaa tagattatct ccaccccatg ctcgagtcta ttctacgaac aacttatggt
     7561 gtaattgttt atcaagagca ggttatgcaa attgcacaaa agttagccaa atatacacag
     7621 ggcagtgctg atattctcag gaaagcgatg ggtaagaaaa ttcctgagga aatggctaaa
     7681 caaaaggatg tttttatcca aggcgccata gataatgatg ttaaggagag aaccgcaata
     7741 aaaatatttg acttgataga gaagtttgca ggttatggtt ttaataaatc acactctgtc
     7801 tcctatgcct tcatcgccta tcaaacagcc tggctaaaaa cgcattaccc agctcaattt
     7861 atggctgctt caatgacagc agatattaat aacacggata agataattcc attaaaagaa
     7921 gattgcacta atttagatat tgaaattcta ccaccttgta ttaatcactc agcttatgat
     7981 ttttttgtac aagattctga gtccattaga tacggcttag gtgccattaa aggtattggc
     8041 gaatctgcag ctgaagcgat gacaagcgaa agattaacaa acggtaccta taaagatttt
     8101 tttgattttt gctctagact taccgatcaa aagctatcaa aaagaacgat agaagcatta
     8161 gtcaaaagtg gggcaatgga ttgtttgaat gaaaacaggt ccacgttgtt caggtcgatt
     8221 ggaactggag tcaatttcgc aaataaaaaa gcaatggatc aaaaatcagg tcaaaataat
     8281 ttattttcca ttgatggtga tcacgctgaa actcctccta gtctaaatag ggataaagaa
     8341 tggaagttaa atgatttatt acaaggtgag tttcaaagtt tgggttttta ttttagcggt
     8401 cacccatttg atccatatag aaaagattgt atccatctaa ctaaaggacc attaatacag
     8461 ataaaagcaa agatggaaaa aagtaataac ggatcactgc tatttaatag tcaaaatttc
     8521 atggaaattg caggtttagt ttttgacgta aaacggagag gaaataatct tatatttcga
     8581 ctagatgatg gcactgcaat cattgatgca attgtttttg gtgagaatag agacagattt
     8641 agagatttga ttaataacaa cgctatatta catcttaatg gaagctttcg ctatgatact
     8701 tatgctgaca catggcaatt tgttgcctca gagattttgg aattagatgc attgatagaa
     8761 aaaaaagcta aaacgctctt aattaaatgc aatccagatt ttgacccaga aaaacttaaa
     8821 tcaattcttc aggcccatac tccaggttct tgtaaagtta acgttcacta taaaactgat
     8881 attgacgttt acaaattaaa atttggccaa gaatggactg ttaaagtgac caaggaactg
     8941 agggatagat taacggctga gtttggttca ggcaactttc agttcttgac gtaccactaa
     9001 attcttatat ttttttataa gatttttttt attttttcta atgtagaatg atagatatat
     9061 tttaatataa tattttacat tatggatttt ttgaattttg aacaacccat tgttgagtta
     9121 gaaagaagaa ttgaggaact taagctaagt gttacagatt cagagtattc tgaagatatt
     9181 gataaagaaa tcaataagtt aagtcaaaaa tgtgaagctt taactaataa aatattttcc
     9241 aatctgactg aatggcaaat cgttcagtta tcaaggcatc cgaacagacc aaaatcttca
     9301 gatgtaatta aagccataac acccgatttt caggagttgc atggcgatag aatgtatgca
     9361 gatgataccg cgattatttc tggtttagga accatagata atttatccat gatgttgata
     9421 gctcaggaga aaggtaagga tacaaaatca agggtgtata gaaactatgg catgcctagg
     9481 cctgagggtt atcgtaaagc acttagagta atgaaacttg ctgagaaatt ttcaattcca
     9541 atagtcacca taatcgatac tccaggagca tatccaggta ttggtgctga agagagagga
     9601 caaagtgaag caattgcaaa aaatctcttt acactggcat cgattagaac tccagtgatt
     9661 aatctggttc tcggtgaagg tggatcaggg ggcgctttag ccataggcat gggggattca
     9721 cttatgatgt tgcaatacag tgtgtacact gtaatttctc ccgaggcttg ttcttctatt
     9781 ctatggagga atcctgaaga aactgaaatt gcagcagatt ccatggggct ttcggcaaaa
     9841 agattatcta aattaggagt tgtagaccaa gtgatcaaag aacctttagg aggctttcac
     9901 cgtgatccag aacctatgta tcagcaaatc accaaagcca tagttgatga agtaagttta
     9961 ctgagcaata tgaacatgtc agatctgttg gagcgaagga gagttaaatt tagagccatt
    10021 ggacaattta aagagattta atcaatggtt gtaagtaaaa atatagatct cgaagagacc
    10081 ttaataaatc atattcaaag actcaattta acaaaaatct gtgtgtcatt gagtggtgga
    10141 ttagattcca ttgttttgct gcatatcctc catc
//
LOCUS       DASCTH010000008         9169 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000014249, whole genome shotgun sequence.
ACCESSION   DASCTH010000008 DASCTH010000000
VERSION     DASCTH010000008.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000014249"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="QGH35_00145"
     CDS             complement(<1..199)
                     /locus_tag="QGH35_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HJL80976.1"
                     /translation="MLDKLVIANRGEVALRIQRACSELNIKTVAIHSTSDSNQKHVLL
                     ADESVCIGPPESVNSYLNIPSI"
     gene            complement(200..649)
                     /gene="accB"
                     /locus_tag="QGH35_00150"
     CDS             complement(200..649)
                     /gene="accB"
                     /locus_tag="QGH35_00150"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008956426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HJL80977.1"
                     /translation="MDIRKVKKLIQLLEESGLSEIEITEGEDTVRITREVSKDSIQTE
                     VVTSHPQQETPDNNQVRNVDQHNNNDLHKIIAPMIGTFYHSSEPNAETFVKVGDEVNE
                     GDTLCIIEAMKMMNQIESDVHGIIERILVQNGDPVEYDQVLFLISPS"
     gene            complement(654..1094)
                     /gene="aroQ"
                     /locus_tag="QGH35_00155"
     CDS             complement(654..1094)
                     /gene="aroQ"
                     /locus_tag="QGH35_00155"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020210217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HJL80978.1"
                     /translation="MVTILVLHGPNLNLLGTREPEIYGDKNLHQIEKELSRIADEHDC
                     NLESFQSNSESDLVDKIQERSQSIDYMIINPGAFTHTSIALRDVLLAVEVPFIEVHIS
                     NIFSREEFRKNSYFSDIAQGTICGLGFKGYELALLSALDQLSNS"
     gene            complement(1197..1634)
                     /locus_tag="QGH35_00160"
     CDS             complement(1197..1634)
                     /locus_tag="QGH35_00160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HJL80979.1"
                     /translation="MNIGFQRHQPQYIVPFALEDLDGNIRDISEWSEQRILINFWATW
                     CAPCRREMPMLQTLHLNQQEHGIQIIGIAVDQNELARDFINEYGISFPILIGQSNAYE
                     IMKGLGNTSLTLPYTLLVEPSGLITWYKNGEVTSQDLPMILGD"
     gene            complement(1754..3547)
                     /gene="dsbD"
                     /locus_tag="QGH35_00165"
     CDS             complement(1754..3547)
                     /gene="dsbD"
                     /locus_tag="QGH35_00165"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HJL80980.1"
                     /translation="MKTTNINSNFCVVSRILLFSLLLFFTALTFAQKVLKPEEAFPVE
                     VIYKDQRLQISHEITVGYYLYKDKISYDSIDQNINLGTTQLPPGKAHFDEFFGNTEIY
                     RDAFIVDIPVDIDATTIMDNALFIEIKLQGCADIGLCYPPQTWQKEISLNQENDGGMI
                     RDNVVNQSEQGRLGNLIAEGNIFLVCITFLGLGFMLAFTPCHLPTIPILSSIILGQTG
                     ETNTLKSLSLSLTYVAGMAITYSAAGIMAALAGQQLQAVFTLPVVLIAIAILFTWLGL
                     SMLGRFNIQIPSLFMNKINTLQAQQKGGTYVGVLMMGGLSALLVTACVAPPLVATLIV
                     IGQSASIMRGILALSSLSLGLGIPLILIGLTAGKWLPRTGDWLNSIKEIFGLIMFGLA
                     IWILNPLMSQDALAIVWALFFVVVGIYLGGLKLNAIILKRISSIRIYTGFVVMLIGFA
                     LLFNQTIGIKSINENENENLYPDADSLFTTIRSIEDLNQSLNMASSNHQITLLYYTAD
                     WCVSCRQLERDTFTNQALIDLLSEINSIKADLSLNNDNDKELMNSFGVYGPPTMLLFN
                     NQGLEQTHMRKIGMVKAEELLGNLNELKLIP"
     gene            3729..4019
                     /gene="groES"
                     /locus_tag="QGH35_00170"
     CDS             3729..4019
                     /gene="groES"
                     /locus_tag="QGH35_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HJL80981.1"
                     /translation="MNIRPLQDRVIIRRMEEETTSPGGIVIPDSATEKPIRGEVVAVG
                     KGLVLDNGDVRPLDLKAGDKVLFGKYSGTEVKIDGEELLVMKEDDVMAVFEG"
     gene            4089..5735
                     /gene="groL"
                     /locus_tag="QGH35_00175"
     CDS             4089..5735
                     /gene="groL"
                     /locus_tag="QGH35_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012981706.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HJL80982.1"
                     /translation="MAAKEVKFGNSARQRMIAGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTVTKDGVSVAKEIELEDNFENMGAQMVKEVASQTSDEAGDGTTTATVLAQAIFRE
                     GIKSVSAGFNPMDLKRGIDKASVVVVESLESMSVPCTDDISIAQVGTISANSDTAVGE
                     IIAEAMQKVGKEGVITVEEGQILENELDVVEGMQFDRGYLSPYFINQADSQSVELDDP
                     YILLFDKKISNIRDLLPLLEGVAKSGRPLLVIAEDIEGEALATLVVNNIRGIVKVAGV
                     KAPGFGDRRKAMLQDIAVLSGGQVISEEVGLSLEKTTLDELGSAKKVQITKENTTVID
                     GAGSASDIKGRIDQINAEIEDSTSDYDKEKLQERLAKLSGGVAVIKVGAATEIEMKEK
                     KARVEDALHSTRAAVEEGIVPGGGVALVRARESLKDLQGDNDDQNVGIKILERALEEP
                     LRQIVSNAGNDASVILNDVVKGKGNYGYNAATEEYGDLVEMGIIDPTKVTRYALQNAA
                     SVTGLLITTEAMISEAPKEDDGPAAPMPDMGGMGGMGGMM"
     gene            5838..6047
                     /locus_tag="QGH35_00180"
     CDS             5838..6047
                     /locus_tag="QGH35_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HJL80983.1"
                     /translation="MFRKITDIQTMTTNKEKLIDITSEDLPLSCPMKEYDDWDAHPRV
                     FLAVESKQTICPYCGTIYQMNDFDD"
     gene            6040..7092
                     /locus_tag="QGH35_00185"
     CDS             6040..7092
                     /locus_tag="QGH35_00185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HJL80984.1"
                     /translation="MINEKPILCHVNLATGYRGGERQTQLLMKQLSSLGWQQMLIARK
                     AGILAQRSGSIDNLIIYETSLNPLQYFRLLSQADIIHLHESRAFLALWLSKISSDAPF
                     ILTRRVQRYPSLGWLNKNIYSKANAIVTISDAIGQGIYRLFDLNYTVIPDAKTSFEFN
                     PEEVKAIRSRVSGDFIVGHIGALDDSHKGQCQIIEVAQKLNVSHPNIAFIIVGQGRDY
                     QLFKKQSSGLENIQFVGQVENVGDYLESFDMFLFPSRHEGLGSILLDAMDFALPVIAT
                     DVGGIPEVIEHAVNGFIVQPNAIEDMIENILRLFSNDDLLQSIGEKNKEKAQAYSVAS
                     MSKQYAQIYNKNINHN"
     gene            7111..8067
                     /locus_tag="QGH35_00190"
     CDS             7111..8067
                     /locus_tag="QGH35_00190"
                     /inference="COORDINATES: protein motif:HMM:NF005292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HJL80985.1"
                     /translation="MDLKFKKDVDASLVRIKNHIRRTPVQFSHYLSNSTGSDVYLKLE
                     NYQITGSFKARGALNKMTLLLKQKPEKVITASSGNHGSAVAYASSVLAMKTLIFLPET
                     VSAVKLNNINQYGTQVHIGGNDTGKAERAARVFANEHDYPYLSPYNDYDVIAGQGTAG
                     AEIVEQIRDVDAVFIAVGGGGLITGIGAYLKLINPNIQVIACSPEHSAAMHHSLEAGK
                     IIDINHKETISDGTAGALEEHSVTYEYCRSIVDQSIVLSEDEIIIAMKEFMNSHQMMI
                     EGAAGVALAGFIKIQQQFQNKRVLIIVCGSNISTEKLMEVLS"
     gene            8064..9023
                     /locus_tag="QGH35_00195"
     CDS             8064..9023
                     /locus_tag="QGH35_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014476.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HJL80986.1"
                     /translation="MSDVIVLDWKQISDLLKNIDVYEPMKKAFIEYSLGHAEIPPVGE
                     LLFADPPGEVHIKYGYIQGGSHYVVKIASGFANNRAQNLTPGQGMMILFDINTGIPKA
                     VLIDDANLTDLRTGIAGAIASKAMVNIGVKSVAIIGTGVQARHQAKCLLDVMPLEAIY
                     CWGRNEKRLDELKRDLSDLDVSITVCSDLRELIGHSQLIITTTSAKEPIIDMEWVQPG
                     THITAVGSDTPEKCELDPIILDRASVVVADSISQNKERGEIHQGLKLGTVKEEDILEL
                     GDIFAGNTEGRTDRDQITVVDLTGVAVQDLVIAEAVVKAKKSY"
BASE COUNT         2879 a         1621 c         1903 g         2766 t
ORIGIN      
        1 tgatagacgg tatattcaag taactattga ctgattctgg aggtccaata cagacggatt
       61 catctgcgag caggacatgc ttttgattac tatctgaagt ggaatgaata gcaacggttt
      121 taatattgag ctcagaacac gctctttgta tcctcagtgc aacttcacca cggtttgcta
      181 tgacaagctt atctagcatt tagcttgggc tgattagaaa taagacttga tcgtactcaa
      241 cgggatcacc gttttgaact aaaatccttt caataatccc atgtacatct gattcaatct
      301 gattcatcat tttcatcgcc tcgatgatgc acagtgtgtc accctcattc acttcatcac
      361 ccactttaac gaatgtttcg gcattgggtt ctgaagagtg ataaaaggtt ccaatcattg
      421 gagctataat tttatggaga tcattgttat tgtgctggtc tacatttcta acctgattat
      481 tatcaggagt ttcctgttgt gggtgtgatg ttaccacttc agtttgaatg gaatctttag
      541 atacttcacg ggtaattcta acggtgtctt caccttctgt aatttcaatt tcagataacc
      601 cagattcttc aagtaactga attaattttt ttacttttct aatatccatg aatttaggaa
      661 ttggataatt gatccaatgc agataataga gccagttcgt aacctttaaa tcccaaacca
      721 cagatggttc cttgtgcaat atcggagaag tatgagttct ttctaaattc ttccctggaa
      781 aaaatattgc ttatatggac ctcaataaaa ggaacctcaa ctgccaaaag tacatccctt
      841 agagcaatgc ttgtgtgggt aaaggcccct gggttgataa tcatataatc tatagattgt
      901 gatctttctt gaattttgtc gaccaaatct gattccgagt tgctttgaaa agactctaag
      961 ttacaatcat gttcatctgc aatccttgag agttccttct ctatttggtg aaggttttta
     1021 tcaccataaa tttcaggctc cctggttcct aggaggttaa gattgggacc atgaaggact
     1081 aaaatagtaa ccatgatatt tgcacgttta aagaagatag caccattttt atagaaaata
     1141 gctataaaat caacgaaaaa atatgattat tattatgaat aatttttatt tcaaattcag
     1201 tcacccaaaa tcataggaag atcttgggag gttacttctc catttttata ccaagttatt
     1261 aaaccacttg gttcaacaag taaggtatat ggcagggtta gcgatgtgtt tccaagtcct
     1321 ttcataatct cgtatgcatt actttgccca atgagtatgg gaaagcttat gccatattca
     1381 tttataaaat ctcttgcaag ctcattttga tcgacggcta tgcctatgat ttggatacca
     1441 tgctcttgtt gattgagatg aagcgtttgc aacattggca tttcccttct gcaaggtgcg
     1501 caccatgttg cccaaaaatt aatcagaatt ctctgctccg accattcact gatatctcta
     1561 atattgccat ccaaatcttc taatgcaaaa ggaacaatgt attgaggttg atggcgttga
     1621 aaaccaatat tcattagttt tatatctgag gttaagacat aaacacttaa ggaaataggg
     1681 actaataaaa ttaaaaatat tgttctcagt tttaattttt ttaacttttt tttcaaaatc
     1741 aatacgtttt gattcacgga atcaatttaa gttcattcaa attacccagt agttcctctg
     1801 ctttgaccat cccgatcttt ctcatatggg tttgttctaa tccctgatta ttaaagagca
     1861 acattgtagg tggaccgtaa acaccaaaac tattcattaa ctctttgtcg ttatcgttat
     1921 tcaatgaaag atctgctttg atactgttga tctctgataa tagatcgatc agagcttgat
     1981 ttgtaaatgt atctctttct aattgtctac atgatacaca ccaatcagca gtgtaataga
     2041 gcaatgtaat ttgatgatta ctcgatgcca tattgagact ttgattcagg tcctcaatgg
     2101 atctaatagt tgtgaataat gaatctgcat cgggatataa attttcattt tcattttcat
     2161 tgatactctt aattccaatc gtttgattga acagtagtgc aaaaccaatc agcataacca
     2221 cgaaaccagt gtatattctg atactactga ttcttttcaa aataatagca tttaatttta
     2281 gtcctcctaa atatatgcca acaacaacga aaaagagcgc ccaaacgatt gcaagcgcat
     2341 cctgactcat taaaggattg agtatccaaa tagcaagacc aaacataatc agcccaaaaa
     2401 tttctttaat ggaattgagc caatcacctg ttctgggcag ccatttacct gcggtgaggc
     2461 caatcagaat taatggaata ccaagtccta agcttaatga tgatagtgct agaatacctc
     2521 tcatgatgct agcgctttgg ccaatgacta ttaaagtagc aactaaagga ggtgctacac
     2581 aagcagtgac taataatgcg gatagtccac ccatcattaa aacaccaaca tatgtgccac
     2641 ccttttgttg ggcttggagt gtattaattt tattcatgaa taagctgggt atttgtatat
     2701 taaaacgtcc tagcatgctc aggcctaacc aggtaaaaag aatagctatt gcaataagaa
     2761 caacaggcag tgtgaaaacc gcttggagtt gttgccctgc tagtgctgcc ataattcctg
     2821 cagcagaata ggtgatggcc atacctgcaa catatgttaa ggagagtgac agacttttta
     2881 gagtgttagt ttcacctgtt tgacccagaa taatgctaga gagtattggg atagtgggta
     2941 gatgacatgg ggtgaatgcc aacataaaac caagtccgag aaaggtgata catactaaga
     3001 aaatattgcc ctctgcaatt agattcccaa gtctcccttg ttcagactga ttgacaacat
     3061 tgtctctaat cattccccca tcattttcct gattgagaga gatttctttt tgccaagttt
     3121 gcggtgggta gcacaatcca atgtcagcac agccctgtaa tttaatttct atgaacaatg
     3181 cattatccat gatagttgtt gcatctatgt ctactggaat atcaactata aacgcatccc
     3241 tataaatttc tgtattccca aaaaattcat caaaatgggc tttacctggt ggtaattgag
     3301 ttgtccctaa attgatgttt tgatctatgc tgtcatatga aattttatct ttgtacaaat
     3361 agtacccgac tgtgatttca tgggatattt gtaatctttg atctttatat attacctcaa
     3421 caggaaatgc ttcctcgggt tttaaaactt tttgtgcaaa tgtaagtgca gtaaaaaata
     3481 acagtaaaga aaacaataaa atcctactta caacgcaaaa attggaattt atatttgttg
     3541 ttttcatctt cgattagcat cttattattt tttgaaaaaa aatgttatca gaaaaaaatc
     3601 gtattctgac ttgaaatttt caaaaccatc attattatta gcactccctt agtgagactg
     3661 ctaaatacgc tagtgaacta aggaataata aagtatatac agaaatgaac aaattaagga
     3721 gctgatagat gaatattcgt ccacttcaag atcgtgtaat cataaggcgc atggaagagg
     3781 aaaccacctc tccaggtgga atcgttatac ctgatagtgc aactgaaaaa cctataagag
     3841 gggaagtcgt tgcagttggc aagggtctag tcctagataa tggcgatgtt cgtccattgg
     3901 atttaaaggc tggagacaaa gttctgtttg gcaaatatag cggaactgag gtcaaaattg
     3961 acggtgagga gctccttgtg atgaaagaag acgatgtaat ggctgttttt gaaggctaat
     4021 tttacatcca atcaactaag catatataaa tcttaatcaa ttaactaaat taaagaggta
     4081 ataatcaaat ggctgctaaa gaagttaaat ttggcaatag cgctcgtcag agaatgattg
     4141 ctggtgtaaa tgtactagct gatgctgtaa aagtaacact aggtccaaaa gggagaaatg
     4201 ttgttctaga taaaagtttt ggagctccca cagttaccaa ggatggtgtt tcggttgcta
     4261 aggaaataga actggaagac aattttgaaa acatgggtgc acagatggtt aaggaagtgg
     4321 cttcccagac ttcagacgag gctggtgatg gaacgactac tgcgaccgta ctggcgcaag
     4381 ctatttttag ggaaggcata aaatctgttt ctgcagggtt taatcctatg gatctcaagc
     4441 gtggcataga caaagcctca gtggttgtag ttgaatctct tgaatctatg tcggtacctt
     4501 gcacagatga tatctcgatt gctcaggtgg gtactatttc tgctaattca gacaccgctg
     4561 ttggtgagat cattgccgag gccatgcaaa aagtgggcaa agagggcgtt atcacggtgg
     4621 aggaaggaca aattctagag aatgaacttg atgttgttga ggggatgcag tttgaccggg
     4681 gctatctatc tccctacttt atcaatcaag cagacagcca aagtgttgaa ctcgatgatc
     4741 cctatattct tctgtttgat aagaaaattt caaatatcag ggatttattg ccgttacttg
     4801 agggcgtcgc caaatcaggc agaccattat tggtcattgc tgaggatatt gaaggtgaag
     4861 ctttagcaac gctggtggtc aataatatcc gaggtattgt gaaagttgct ggtgtcaaag
     4921 cacctggttt tggtgatcga agaaaagcaa tgttacagga tattgcggtt ttgtcaggcg
     4981 gtcaggtgat atcagaagaa gttggcttga gtcttgaaaa aacaactttg gatgagttgg
     5041 gcagtgccaa aaaagttcag atcactaaag agaatacgac agtcatagac ggtgctggaa
     5101 gtgcttctga tattaaaggg aggattgatc aaatcaatgc tgaaatcgaa gattcaacat
     5161 cagattatga taaagaaaaa ctccaagaac gccttgctaa actctctggt ggtgtagcag
     5221 ttattaaggt aggtgctgca actgaaattg agatgaaaga gaagaaagct agagttgaag
     5281 atgccttaca ctcaactaga gctgctgtag aagaaggaat tgtccctgga ggtggtgttg
     5341 cacttgtcag agcaagggag tcactgaaag acttgcaagg ggataatgat gaccaaaatg
     5401 ttggtatcaa aattctagaa agagctttgg aagagcccct tcgacaaata gtcagcaatg
     5461 ctggaaatga tgcctccgtt atccttaatg atgttgtcaa aggtaaggga aattatggat
     5521 acaatgcagc gactgaagag tatggtgatt tggttgaaat gggtatcatc gacccgacca
     5581 aagtaacacg ttatgctttg caaaatgctg catctgttac cggtcttcta ataacaactg
     5641 aagccatgat ttcagaagca cccaaggagg atgatggacc agctgcacca atgcccgata
     5701 tgggtggcat gggtggtatg ggtggcatga tgtaactctc aacctgcatc caaaaaaatt
     5761 aaagccctgc tgtcgcaggg ctttttttta ataatctccc agatacaact ttagtgtttc
     5821 attgagctgg tctatcattg ttcaggaaga tcacagatat acaaacaatg acaaccaata
     5881 aagaaaaatt gattgacatc acatcagaag atttgcccct gtcttgtcca atgaaagaat
     5941 atgatgattg ggatgcccac ccaagagttt ttctagctgt ggaatctaaa caaactattt
     6001 gtccgtactg tggaactata tatcaaatga acgattttga tgattaatga aaaaccaatt
     6061 ctttgccatg ttaatttagc aacagggtat cgaggcggtg agcgacagac gcaactttta
     6121 atgaaacaac tgagttcttt gggttggcaa caaatgctca ttgcaaggaa agcaggaata
     6181 cttgctcaac gttcaggatc cattgacaat cttataattt atgaaaccag tttaaatcct
     6241 ttacaatatt tccgtttgct ctcacaagca gatatcatcc acctgcatga atctagggca
     6301 tttctagcct tatggttatc taaaatttca agtgacgctc cattcattct gacgcgaaga
     6361 gtgcaaaggt atcctagctt gggctggctc aataagaata tttattctaa agccaatgca
     6421 atcgttacaa tatccgatgc tattggccaa ggaatttata ggctgtttga tctcaattac
     6481 acagttattc cagatgcaaa aaccagcttt gaatttaatc ctgaagaggt aaaagctatt
     6541 cgatcaagag tgtcaggtga tttcatcgtt gggcatattg gagctttaga tgatagtcac
     6601 aaagggcaat gtcaaatcat tgaggtcgct cagaaattaa atgtctcaca ccctaatatt
     6661 gcttttatca ttgtaggtca aggtagagat tatcaattat tcaagaaaca atcatctggg
     6721 ttagagaata ttcaatttgt aggccaagtg gaaaatgttg gcgactatct agagtcattt
     6781 gacatgtttt tatttccatc tcgtcatgag ggtttgggtt ctattttatt ggatgctatg
     6841 gattttgctt tgcctgtgat tgcaaccgat gtggggggta ttccagaagt tattgaacac
     6901 gccgtcaatg gatttatagt tcagccgaat gccattgaag atatgattga aaatattctt
     6961 cgactgtttt ctaatgacga tctgttgcaa tcaattggtg agaagaataa agaaaaagca
     7021 caagcatata gtgttgcttc gatgtcaaaa cagtacgctc aaatttataa taaaaatatt
     7081 aatcataatt aactacttca ggtcaattgc gtggatttaa aatttaaaaa agacgtcgat
     7141 gcttcattag taagaatcaa gaatcacatt cgtagaacac cggttcaatt ctctcattat
     7201 ttaagcaata gcacaggcag tgatgtttac ttgaagctag aaaactacca aatcacaggg
     7261 tcctttaaag ctagaggagc gctcaacaaa atgacattat tattgaagca gaagcctgaa
     7321 aaggtcatca ctgcctcttc tggcaatcac ggctcagccg tggcctatgc ttcgagcgtt
     7381 ctggcaatga aaactctgat tttcctgcca gaaacagtat cagctgttaa gttaaataac
     7441 atcaaccaat acgggacaca agtccatatt ggtggtaacg ataccgggaa agcggaaagg
     7501 gcagcaagag tctttgcgaa tgaacatgat tatccttacc tgtctcccta taacgactac
     7561 gatgtcattg ctggtcaagg gacagctggt gcagaaatag tagaacagat cagagatgtt
     7621 gatgcagtat ttattgctgt cggaggggga ggattaatta ctgggatagg tgcttatctg
     7681 aaactcatca atcctaacat ccaagttatc gcttgttcac ctgagcattc tgcagcaatg
     7741 caccattctc tggaagctgg caaaattatt gatattaatc ataaagaaac gatcagtgat
     7801 ggaactgccg gtgcattgga agaacattca gtgacttatg aatattgtcg atctatcgtt
     7861 gatcaatcta ttgtattgtc cgaagatgaa attattattg ccatgaaaga atttatgaat
     7921 agccatcaaa tgatgattga aggtgccgca ggagtagctc ttgctggttt tatcaaaatt
     7981 cagcaacaat tccagaataa acgagtttta atcatcgtct gtggctcaaa tattagtact
     8041 gaaaagctaa tggaggttct ttcatgagtg atgtaatagt tcttgattgg aagcagattt
     8101 cagatttgtt gaagaatatt gatgtctatg agcccatgaa aaaagctttt attgaatatt
     8161 cattaggtca tgctgagatt cctccagttg gggaacttct atttgcagac cctcccggtg
     8221 aggtccatat caaatatggt tatattcaag gaggctctca ttatgtcgtt aaaattgcct
     8281 ctggttttgc caataatcga gctcaaaact tgaccccagg acaaggaatg atgattcttt
     8341 ttgatatcaa tacaggaatt cccaaagcag tacttattga cgatgctaat ttgacagatc
     8401 taagaacagg tattgctgga gccattgctt ccaaagcaat ggtcaatatc ggagttaaat
     8461 ctgttgcaat aattggcact ggagttcaag cgagacatca agccaagtgc ctgttggatg
     8521 tcatgccgct ggaggcaata tattgttggg gaaggaatga aaaaaggctg gatgagctga
     8581 aacgagatct ttccgattta gatgtcagta tcactgtatg ctctgattta agggaactga
     8641 tcggacactc tcagttaatc attaccacca catccgccaa ggagcctatt attgatatgg
     8701 aatgggtgca accaggaacg catataaccg ctgtgggttc agatacgcca gagaaatgtg
     8761 agttagatcc aataatactt gatcgagcat cagttgtggt tgctgacagt atttcacaaa
     8821 ataaagagag aggtgaaata caccagggac tgaaattagg gacggtgaaa gaagaggaca
     8881 ttttagaact gggagatatt tttgccggta ataccgaggg gagaacagat agggatcaga
     8941 tcacggttgt cgatttaact ggagtcgcag tccaggattt ggtcattgca gaagcagttg
     9001 tgaaggcaaa aaagtcatat tgactgactg tgtgtaatcg taacaattgc tatttgtgat
     9061 ccagttctga ggaaatcaat cttttgaaga gcaagcaaaa aaactatttg ttttatatag
     9121 aacatgatta ttcttatgag atcttaagac cgcttcaaaa ggagattca
//
LOCUS       DASCTH010000009         3053 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000017795, whole genome shotgun sequence.
ACCESSION   DASCTH010000009 DASCTH010000000
VERSION     DASCTH010000009.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000017795"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            170..1171
                     /locus_tag="QGH35_00200"
     CDS             170..1171
                     /locus_tag="QGH35_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HJL80987.1"
                     /translation="MKAFLCRKFGPIDSHQVEEVDDLVPAKHEVLVEVYAAGVAFPDV
                     LVVQGLYQFKPAFPFSPGGEFAGVVKEIGEGVQRWKIGDRVIGFKGSGGFAEQALCNE
                     FNLMPLPDAMEYLTGAIFPMNYGTSIYALKQRAELQSGETLLVLGAGGGLGLTALHVG
                     KAMGAKVIAAASTPEKLELCSQQGADATILYSRESMDTDSQKEFSNELKRLTEKKGVD
                     VIFDPVGGSYSEPALRAIAWKGRYLVIGFTSSIPSIPLNLALLKGCQIVGVFWGNFCI
                     KEPLVNAANFQELFEMYGAGKIKPFAVKMFALEDTAAAIATLANREAVGKVAISIRD"
     gene            1172..2476
                     /locus_tag="QGH35_00205"
     CDS             1172..2476
                     /locus_tag="QGH35_00205"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL80988.1"
                     /translation="MKQSASYGLLGFLTAINILNFIDRQLLTSFSNFIVPDLGLTNTQ
                     FGLLTGFAFIIFYSLMGLFMGAIADRVHRPRFISFGVALWSILTAVSGMAKNFWMLFI
                     PRIFIGIGESILTPTALSLLSDRFPKRNMGFVAGVYYLAVPLGISASLFTAGYLGPIL
                     GWRNCFYLLGLLGLFFALFMLLVKETPHQQTDINNQLKSDVINRKSFHEAITVLRTSL
                     QQYVSLKWTIYGGIAVHFILGAMVFEQLWFVEEKGFERAEIARIAGFMGLTGGVMGNI
                     FGGVVGDYYAKVTGQGRQMFLFWCILLFVPANIIYRITDDNGFIFFTCMFLGFFQWGC
                     MYGPVFSSVQEMAPVRIRAVIVAYYLLMVNIIGLGFGITLSGIMIDYLISQGYEEPYS
                     VTLLSFQLLSGLSLPALYYAGKNYKSDREQMLAQENINSLPQ"
     gene            complement(2457..>3053)
                     /locus_tag="QGH35_00210"
     CDS             complement(2457..>3053)
                     /locus_tag="QGH35_00210"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJL80989.1"
                     /translation="PWDQRMCLVPDGDLFNAIKSGKASVVTDHIERFTKTGIKLKSGD
                     ELETDFIVTATGLNLLVCNGIELFVDNEQIDVSKKMTYKGMMFNDVPNLVATFGYTNA
                     SWTLRADLTSEYVCRLVNFMDKRNFDYCCPRVDGVVEEDGNWLDFSSGYVVRSMHEFP
                     KQGSREPWRNTQNYLKDIFAIRFGRLQNKELKFTVAKN"
BASE COUNT          842 a          568 c          665 g          978 t
ORIGIN      
        1 atctttaagc taaaacaaag tgacttaagc gaagttgcag tgttttcatt ttctaacacc
       61 gtaatgggtt cggtgacggt agcagttcca gatgaggata gagtatggct tggtaccttt
      121 catggcgata gaatggcaag ctttaatatt gactaaataa gagaaaataa tgaaagcatt
      181 cttatgtaga aaatttggac ccattgactc acatcaggtt gaggaggttg atgatttagt
      241 gcctgctaaa catgaggttt tggttgaagt atacgccgca ggtgttgcct ttccagatgt
      301 cttagtggtt caaggacttt atcagtttaa gccggcattt cctttttcac caggaggtga
      361 atttgcaggt gtggttaaag aaatcggtga aggggttcaa cgatggaaaa taggagatcg
      421 agtcattgga tttaaaggta gtggcggctt tgcagaacaa gcactgtgca atgaatttaa
      481 cctgatgcct ctaccagatg caatggagta tctaaccggg gcaatttttc caatgaatta
      541 tggaacttcg atctatgcat tgaagcaaag agccgaatta caatccggag agacactgtt
      601 ggtattgggc gcaggaggtg gattgggtct tactgccctc catgttggta aagccatggg
      661 agctaaggtc atagcggctg catcgacacc agaaaaatta gaattgtgtt cccagcaagg
      721 tgctgatgcc accattcttt attcaagaga gagtatggat actgactctc agaaagagtt
      781 ttccaatgaa ttgaaacgct taaccgagaa aaaaggggtg gatgtgattt ttgatccggt
      841 tggcggatcc tacagtgagc ctgctttaag agcaattgca tggaagggca ggtacttagt
      901 gattggtttc acatcaagta ttccgtccat acctctcaac ctagcccttt taaaaggttg
      961 tcagattgtt ggtgtttttt ggggtaattt ctgtatcaaa gaacccttag tgaatgctgc
     1021 aaactttcaa gaactctttg agatgtatgg agcaggtaaa atcaaacctt ttgcagtgaa
     1081 aatgtttgct cttgaggaca cagctgcggc catagcaacc cttgctaata gagaggctgt
     1141 tggaaaggta gcaatctcca ttcgtgacta aatgaaacaa tccgcaagtt atggtttgct
     1201 tgggttccta acagctataa acattctcaa ctttatcgac cgacaactgc tcaccagttt
     1261 ttccaatttt atagttccag atttaggttt aacaaacacc caatttggtc tcctcactgg
     1321 atttgccttc atcatatttt attctttaat gggtttgttt atgggtgcca tagcggatcg
     1381 agtgcatcgc ccaaggttta tctcatttgg tgttgcactt tggagcatcc ttacggcagt
     1441 atctggcatg gcgaaaaact tctggatgct ttttattcca agaatattta ttggaatagg
     1501 ggagtcaatt ttgaccccta ctgctctttc tctgctttct gatcgttttc caaagagaaa
     1561 tatgggattt gtagccggtg tttattattt agcagtaccc cttggaattt ctgcttcttt
     1621 gttcactgct ggttatttag ggccaatctt aggttggcga aattgcttct acttattagg
     1681 tttattgggc ctgttttttg ctctattcat gttattggtc aaagaaacac cccatcagca
     1741 aacagatatc aataatcaac tgaaaagtga tgtcatcaat agaaaaagct tccatgaagc
     1801 cattacagtt ttgcgaacct cactccaaca atacgtatca ttgaaatgga caatctatgg
     1861 gggtatagcc gtccacttta tacttggcgc catggttttt gaacaattgt ggtttgttga
     1921 agagaaaggc tttgagagag ctgagattgc taggatagca ggttttatgg gacttacagg
     1981 gggagtcatg ggaaatatat ttggtggcgt tgttggggat tactatgcaa aagtcactgg
     2041 tcagggcagg caaatgttcc ttttttggtg catccttctg tttgttccag ccaacatcat
     2101 ttaccgaata acggatgata acggttttat atttttcact tgcatgtttt taggcttttt
     2161 ccagtggggt tgcatgtacg gtccagtatt ttcttctgtt caagaaatgg ctcctgttag
     2221 aatcagggca gtcatagtgg cctattatct gctaatggta aatattattg gtctaggttt
     2281 tggcatcacc ttgagtggga taatgattga ttatctcatc agtcaaggtt atgaagaacc
     2341 ctattcagtc actttgctta gttttcaact gttgtcaggt ttgtctctac ctgctttgta
     2401 ttatgctgga aagaactata aaagcgatcg agaacaaatg cttgcacaag aaaatattaa
     2461 ttctttgcca cagtaaattt taattctttg ttttgaagac gtccaaatct gatagcaaag
     2521 atatctttca agtaattctg agtatttctc catggttccc tggagccctg tttgggaaac
     2581 tcgtgcatag acctgactac ataaccagat gaaaaatcca gccaatttcc atcttcttca
     2641 acaacgccat caactctagg gcaacaataa tcaaagtttc ttttgtccat aaaatttact
     2701 agtcgacaga cgtattccga ggtgagatca gccctcagag tccaggatgc attagtatag
     2761 ccaaaagtgg caaccagatt agggacatcg ttgaacatca tacctttgta agtcattttt
     2821 tttgatacat caatctgttc attgtcaaca aacagttcta taccattaca aactaaaaga
     2881 tttaagcctg ttgcagttac gatgaaatct gtttcaagtt catcccctga tttaagcttg
     2941 atccctgttt tcgtgaatct ttcaatatga tcagttacaa ctgatgcttt tcctgattta
     3001 atagcattaa ataaatcccc atctggcact aggcacatcc gttgatccca tgg
//
LOCUS       DASCTH010000010         2123 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000021054, whole genome shotgun sequence.
ACCESSION   DASCTH010000010 DASCTH010000000
VERSION     DASCTH010000010.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000021054"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="QGH35_00215"
     CDS             <1..397
                     /locus_tag="QGH35_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593172.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HJL80990.1"
                     /translation="DFGRRPRILVAKLGQDGHDRGAKVIATAFADMGFDVDIGPLFQT
                     PEEAAKQAVENDVHILGVSTQAAGHKTLVPMLLDALKNEQAENIVVVCGGIIPEQDIP
                     VLKAAGVGAVFGPGTNIAKAAMEVLDLIG"
     gene            404..1936
                     /locus_tag="QGH35_00220"
     CDS             404..1936
                     /locus_tag="QGH35_00220"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HJL80991.1"
                     /translation="MKKNIKELEKRANAAKLGGGEDRIKKQHAKGKLTARERLDILLD
                     EDSFQEWDMFVEHRAVDFGMEEQKIPGDGVVTGYGKINGRLVYVFSQDFTVFGGSLSE
                     AHAEKICKVMDHAVRNGAPLIGLNDSGGARIQEGVVSLGGYAEVFQRNVLASGVIPQI
                     SLVMGPCAGGAVYSPAMTDFIVMVEDSSYMFVTGPDVVKTVTHEEVTAEELGGASTHS
                     RKSGVADLTFKNDTEALLMTREFLSHLPQNNKEKTPIVPTKDPIERAEHSLDTLIPDD
                     PAKPYDIKDVINKIADEGDFFEIQSNYAQNIVIGFIRLNGSTIGVVGNQPLVLAGCLD
                     INASIKGGRFVRFCDAFNIPLLTLVDVPGFMPGTSQEYGGIIKHGAKLLFAYAEATVP
                     KVTLITRKAYGGAYDVMSSKHIRGDINFAWPSAEIAVMGPKGAVEIIFRQDIGNEEKI
                     EKRTNEYREKFANPFIAASRGYIDDIVLPYQTREKLCHSFEMLKNKEQSNPWRKHDNI
                     PL"
     gene            1942..>2123
                     /locus_tag="QGH35_00225"
     CDS             1942..>2123
                     /locus_tag="QGH35_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011425487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HJL80992.1"
                     /translation="MFKKILIANRGEIACRIIKTCNQMGIKTVAVYSDADQNAMHVSR
                     ADESVHIGGSASSESYL"
BASE COUNT          665 a          396 c          494 g          568 t
ORIGIN      
        1 agattttgga agaaggccta ggattttagt tgccaaatta ggtcaagatg gccatgacag
       61 aggagctaaa gtaattgcta cggcatttgc agacatgggt tttgatgtcg atattggccc
      121 tttgtttcaa actccagaag aagctgcaaa gcaagcagtt gaaaatgatg tacatatcct
      181 tggtgtttca acacaggctg caggccataa aaccttggtg ccgatgctac ttgatgcatt
      241 aaagaatgaa caagcagaaa acatcgtggt ggtttgtggt ggcatcatcc ccgaacaaga
      301 catccctgtt cttaaagctg caggtgttgg agctgtgttt ggacccggca ccaatatcgc
      361 caaagcagcc atggaagttt tggatctaat tggttagaag tgtatgaaaa aaaatattaa
      421 agagctggaa aaaagggcta atgctgccaa attaggtggt ggcgaagatc ggattaaaaa
      481 gcaacatgcc aagggtaaat taactgccag agaacggctt gatatccttc ttgatgagga
      541 tagttttcaa gagtgggata tgtttgtcga gcatcgagca gtcgactttg gtatggaaga
      601 acaaaaaata ccaggtgatg gtgtagtcac cggttatggc aagatcaacg gtagattggt
      661 ttatgtgttc agccaggatt ttaccgtttt tggaggatcc ttatcggaag ctcatgcaga
      721 aaaaatttgt aaagttatgg atcacgcagt ccgtaatggt gcaccattaa tcgggctcaa
      781 cgattcagga ggtgcaagaa tacaagaagg agtcgtatct ctgggcggtt atgccgaagt
      841 gtttcaaaga aacgtattgg cctcaggtgt cattccacaa atttccctcg tcatgggacc
      901 gtgtgcaggt ggagcagtgt attcgccagc aatgacagat tttattgtta tggttgagga
      961 cagttcatac atgtttgtca cggggccaga tgttgttaaa acagtcactc acgaagaagt
     1021 tacggcagag gagttgggag gtgcatctac ccacagtaga aaatctggtg tagcagacct
     1081 aacatttaaa aatgacaccg aagctttatt gatgaccagg gagtttctca gccatcttcc
     1141 gcaaaacaat aaagaaaaaa caccaattgt tcccacaaaa gatcccattg aacgagccga
     1201 acactcttta gatacattga tacctgatga tcctgctaaa ccctatgaca ttaaagatgt
     1261 gatcaataaa atcgccgatg agggtgattt ttttgaaata caatccaatt atgcacaaaa
     1321 cattgttatt ggtttcatcc gactgaatgg atccaccatt ggagtggtag ggaatcagcc
     1381 acttgttctt gctgggtgtc ttgatattaa tgcctccatt aaaggtggtc gatttgtccg
     1441 tttttgcgat gcctttaata ttcctctact cactctagtg gatgttccag gatttatgcc
     1501 tggaacctca caggagtacg gtggaatcat caaacatgga gccaaacttc tcttcgccta
     1561 cgctgaagcc acggtaccta aagtcacatt aatcacccga aaagcctacg gcggtgcata
     1621 cgatgtaatg agttcaaaac acatacgagg tgatattaat tttgcttggc catcagctga
     1681 aattgctgtt atgggtccaa aaggtgctgt agaaatcatc tttaggcaag atattggcaa
     1741 tgaagagaaa atagaaaaaa ggaccaatga atacagagaa aaatttgcta atccgttcat
     1801 agcagccagt cgaggctata tagatgatat cgttttaccg tatcaaacac gtgaaaaact
     1861 atgtcactca tttgaaatgt tgaaaaataa agaacagtcc aatccttggc gaaagcacga
     1921 caatataccg ctttagataa aatgtttaaa aagatactga ttgccaatcg tggagagatt
     1981 gcttgtagaa taatcaaaac gtgcaatcaa atgggaatca aaacggttgc agtttattct
     2041 gatgcagacc aaaacgccat gcatgtatca agagctgatg aatctgttca catcggcgga
     2101 tcagcttctt ctgaaagcta tct
//
LOCUS       DASCTH010000011         1710 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000021079, whole genome shotgun sequence.
ACCESSION   DASCTH010000011 DASCTH010000000
VERSION     DASCTH010000011.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000021079"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            122..1591
                     /locus_tag="QGH35_00230"
     CDS             122..1591
                     /locus_tag="QGH35_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HJL80993.1"
                     /translation="MWSVGKPITTGQEYIESLRNRELSVYLFGEQIDDPVEHPIIRPS
                     INALAATYDLAQTNPDLAASVSPYTGEKINRFLHIAENADDLILQNKMQRKLGQLTGT
                     CFQRCVGMDAINSLHSVTYDMDQKYQSDYHQKFLSFLTMVQHGGFVISGAMTDVKGNR
                     NLLPHQQSDPDLYVRVVDRNEDGVFIRGAKAHQTGCINSHWLIVMPTLRLTEKDKTYA
                     IVGAIPVDAKGITYIYGRQSSETRHMEESTIDTGNQKFSGQEALVIFENVFIPNEFVF
                     LDGEYEFAAPLVERFTCYHRRSYVCKSGVGDVLIGAAATIAEYNGVDKASHVKDKLTE
                     MTHLNETIYGVGISSSQESEAMPSGVYMPNDMLANVCKHHVTKLTYEIGRLAQDLAGG
                     LVATMPSEKDLKHPELGKIIQKYLKTKPDLDPEDRVRILRLIENMTLGRNAVGYLTES
                     LHGAGSPQAQRVRIAQQMQLDFKKELARVLAGIIPEPKV"
BASE COUNT          533 a          305 c          373 g          499 t
ORIGIN      
        1 tttgtagttg ggcctaatta ttcacattca ttgaatcttg cagtcaaaaa gagaagtgag
       61 aatgacataa ttcagcccgg tacaggtaag caaaaacgca taaaataatc ttattgttat
      121 aatgtggagt gtgggaaaac cgattacaac tggtcaagag tatattgaaa gtttgcgaaa
      181 tcgtgagctt tctgtatatc tctttggcga gcagatcgat gatccagttg aacaccccat
      241 cattagacct tctattaatg cgttagcagc aacctacgat cttgcacaaa caaacccaga
      301 tttagctgct tctgtttcgc cttataccgg cgaaaagatt aatcgatttc tacatattgc
      361 tgagaatgca gacgatctta ttttgcagaa caaaatgcag agaaaattag gtcaactaac
      421 aggtacttgc tttcaaagat gtgttggaat ggatgctatt aatagtctgc attcagtgac
      481 ctatgacatg gaccaaaaat atcaatcaga ttatcaccag aaatttttat cctttcttac
      541 catggtgcaa catggtgggt ttgttatcag tggagcaatg actgatgtca aagggaatcg
      601 caatttattg ccacatcagc aatctgatcc tgatctttac gtccgagttg tggatcgcaa
      661 tgaagatgga gtttttatcc gtggagcaaa agcccatcaa acggggtgca tcaactcgca
      721 ttggttgatt gtaatgccaa cattaagatt aacggaaaaa gataaaacat acgctattgt
      781 tggagccata ccggttgatg ccaaagggat tacttatata tatggcagac aatcgagtga
      841 aaccaggcat atggaagaat caacgattga tacaggcaat caaaaatttt ctggacaaga
      901 ggcattggtt atttttgaaa atgtttttat tccgaacgag tttgtttttc tagatggtga
      961 atatgaattt gctgcacctt tggtagaacg attcacttgt tatcatcgaa gaagttatgt
     1021 ctgcaaatca ggggtagggg atgttttaat tggtgccgca gctactattg ctgaatacaa
     1081 tggtgtcgac aaggcatcac acgtcaaaga caagctgacg gaaatgacac atttgaatga
     1141 aacaatttat ggggttggca tatcttccag ccaggagtca gaagccatgc catccggtgt
     1201 ttatatgcct aatgatatgt tggccaatgt ttgtaaacat catgtaacaa aattgactta
     1261 tgaaatcgga agactggcac aagatctagc agggggtttg gttgcaacga tgccatctga
     1321 gaaggattta aaacatcctg aattgggtaa aattattcag aaatatctca agactaaacc
     1381 ggatcttgac cctgaagatc gtgtgcgcat actccgtctc attgaaaata tgacattggg
     1441 acgcaatgct gtagggtatt taacagaatc tcttcacggt gcaggctctc cacaagcaca
     1501 gagggttcga attgcacaac aaatgcaatt agactttaaa aaagaattgg cacgtgtcct
     1561 agccggaatt attccagagc caaaggtgta actgttgatt tagttcttca gcagaggaat
     1621 aggatctaat acccaatttt tctcagtgtt tctagctggg caaggaacag aatatgcggt
     1681 ttgatgttcc tcttctttca gtttttttgt
//
LOCUS       DASCTH010000012         2041 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000021229, whole genome shotgun sequence.
ACCESSION   DASCTH010000012 DASCTH010000000
VERSION     DASCTH010000012.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000021229"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="QGH35_00235"
     CDS             <1..361
                     /locus_tag="QGH35_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HJL80994.1"
                     /translation="KLNEKRGLSILFISHDLSLVKSFAHRTMVMYAGRIVESASTDII
                     FPQPKHPYTKSLLQVASLAKENDSFITINGSVPDPRHYPSGCPFHPRCDVVEDVCVKL
                     YPEMFVHNNQNWYCIHE"
     gene            457..>2041
                     /locus_tag="QGH35_00240"
     CDS             457..>2041
                     /locus_tag="QGH35_00240"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HJL80995.1"
                     /translation="MQKKHLFILFALTVLMEGSPYFQQEVAYDINVSLNDSAHTLTAY
                     EKLVYTNHSPDTLHFIWFHLWPNAYKNNETALAKQMMDNFSTRFYYANQDQKGFIDSL
                     DFQTNGVHLKWEFHPDWIDVAKVYLAEPLDPEESVTIETPFFVKLPEVFSRLGHTGKH
                     YEITQWYPKPAVYDKDGWHAMPYLNMGEFYSEFGTFDVKITLPKEYRIMATGDLVDGE
                     KEIAWLDSLAEEGNVLYELDKKAFKKKIKELQRGEKASEKFSFKSLFNKDEEKSENND
                     PIQKIKTLHFHQENVHDFAWFADPKWIVKKGGLWLADSSSIITLWSMYLPKNAKVWEQ
                     SLEYIHDAGYWYSKFYGDYPYNHITAVDGDLSAGGGMEYPNITVISSGNSKDLLEFVI
                     MHEVGHNWFYGIHGTNERDHAFLDEGLNQYSNIRYWEKKYPERNGQMIMQDFVQNKLK
                     IANNLDFRWIMGYLGYQGRAVTGDDQPIDIPSQDFNSSNYGSIVYGKTGIYTRYLQEY
                     LGEEKMDEVMQDYYETWKFR"
BASE COUNT          681 a          329 c          402 g          629 t
ORIGIN      
        1 gaagttgaat gaaaaacgtg gtttgagtat tttattcatt tctcatgacc tgtcattagt
       61 caaatcattt gctcatagaa ccatggtgat gtatgccgga agaattgttg aatcggcatc
      121 cactgatatc atttttcccc aacccaagca tccgtataca aaatctttat tgcaagttgc
      181 cagccttgcg aaagaaaatg actcatttat tacaattaat ggatctgttc cggatccgcg
      241 tcattatcct tcaggatgcc catttcatcc ccgttgtgat gttgtggaag atgtgtgtgt
      301 aaaattatat ccagaaatgt tcgttcataa taatcaaaac tggtattgta ttcatgaata
      361 atgagttttt ctttcaattt ttgttaaagt tttgacaacc atttctttat aaaaccatca
      421 aatataattc agtcactatt taaaaggaaa atatcgatgc agaaaaaaca tctatttata
      481 ctatttgctc taacagttct tatggaagga agtccctatt tccaacagga agtagcctac
      541 gacattaatg tttctttaaa tgattctgct catacgctca cagcttatga gaaacttgtt
      601 tatacaaatc attctccgga tacattgcat tttatttggt ttcatttatg gccaaatgct
      661 tataaaaata atgaaactgc attagcaaag caaatgatgg ataatttttc aacacgattc
      721 tattacgcaa atcaagatca aaaaggattt atagacagcc ttgattttca aacaaacggt
      781 gtacatttaa aatgggaatt tcatcctgat tggattgatg tggcaaaggt ttatcttgca
      841 gaaccattag atcccgaaga aagtgtaaca attgagacac ctttctttgt aaaactcccg
      901 gaagttttca gccgcttggg acacacgggt aagcattatg aaataacgca atggtatccc
      961 aaaccagcag tatacgacaa agacggctgg catgccatgc cctatttgaa tatgggtgag
     1021 ttctacagtg aatttggaac atttgacgtc aaaatcactc ttccgaagga atacaggata
     1081 atggcaacag gtgatttagt ggatggtgag aaagaaatcg cctggctgga ttcactagca
     1141 gaagaaggca atgtccttta tgaattagat aaaaaagcat ttaaaaagaa aatcaaggaa
     1201 ttacaaagag gagaaaaggc ctctgaaaaa ttttcgttta aatctttatt taataaggat
     1261 gaagaaaaga gtgaaaataa tgatccaatt caaaaaatta aaacacttca ttttcaccag
     1321 gaaaatgttc atgattttgc ctggtttgca gatccgaaat ggattgtaaa aaaaggcgga
     1381 ctttggttag cagattcttc aagcataata acattatgga gtatgtattt accaaaaaat
     1441 gcaaaagtct gggaacaatc tttagagtat atccatgatg ctggttactg gtacagtaag
     1501 ttttatggtg attatccata taatcatata actgctgttg atggagattt atctgccgga
     1561 ggcggtatgg agtatccaaa tatcacagtc atttccagtg gtaattctaa agatttgctc
     1621 gagtttgtta taatgcatga agttggccat aactggtttt acggtataca cggaactaat
     1681 gaacgggatc atgccttttt ggacgagggc ttgaatcagt atagtaatat ccgctattgg
     1741 gaaaagaaat atccggaacg taacgggcag atgataatgc aagattttgt tcagaataaa
     1801 ctcaaaattg caaacaacct tgattttcgc tggatcatgg gttatttagg ttatcaaggc
     1861 agggctgtaa ctggtgatga tcaaccgata gatattcctt cccaggattt taacagtagt
     1921 aattatggtt cgattgttta tggtaaaact ggtatctata cacgatactt gcaggaatat
     1981 ctgggggaag aaaagatgga tgaagtaatg caggattatt acgagacctg gaaatttcgc
     2041 c
//
LOCUS       DASCTH010000013         3308 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000021444, whole genome shotgun sequence.
ACCESSION   DASCTH010000013 DASCTH010000000
VERSION     DASCTH010000013.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000021444"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            52..591
                     /locus_tag="QGH35_00245"
     CDS             52..591
                     /locus_tag="QGH35_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL80996.1"
                     /translation="MKQAQAGRIVHVGSKSAYIQAPAVGIDFDNLRGEGVFDSQEAYG
                     RSKLANALFSLELAARLEGSSVTSNVIHPGLVKTNIARTAPIVLRKAFEWFGDFIAKT
                     PQEGAATQVYVATNPALKGVSGAYFEDCNAVTVGGDNHIFDKPMAEHLWATGEEMAQG
                     YLITIEWAVGELAKHKKDD"
     gene            596..1849
                     /locus_tag="QGH35_00250"
     CDS             596..1849
                     /locus_tag="QGH35_00250"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HJL80997.1"
                     /translation="MKSLLKYLFIMVLSSGLTVIALNQFTPIVITIPKKDKQEIKIDF
                     DQALAKKDLKRIDRKAKNIILLIGDGMGSNQVTAYRIAKGGPNYITAFDKFPFTGLVK
                     THAVDTLITDSASSATAYSSGIKTVNGYLGVDKDKQIVGNITEILYEKGYVSSLIATS
                     EITHATPAAFAVHNESRDNTDQIAEAIFKSNNFILLGGGRDFFLPKNLGGQREDKLDI
                     LSLIKEQERYLSSKEDFNNFQFDPSKRIFGLFASEALIRKENEPNLLEMFNFTLEQSE
                     KMLVTNCAGFFIMTEGSQIDWEGHENDFNDQFKEMDEFNQVVDRALEYAKLDTNTLVI
                     VLADHETGGLLIEMDDFRNKPSNKMKISWNTAVGYGTHTGSMIPVYAYGPGAENVTGV
                     IDNTDIFFIMKEALGLDELSDRQCL"
     gene            2042..>3308
                     /locus_tag="QGH35_00255"
     CDS             2042..>3308
                     /locus_tag="QGH35_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013739.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJL80998.1"
                     /translation="MTTENYDVIVIGAGVAGMTAASLLGQDCDKKVLVLERAPFIGGR
                     CLSYVGEGDKVVADGVEMDARAFKKSLPLAHCYLSKCTPGIETIFSEGLLDGRTFEAG
                     GHGLFWGNNNRADCMLKHVGRHVEMPLNRGLGFIEWQGLNDDGTVKPTVAHQVQKGQT
                     YPWMSEEGFAKTRDQLTDMSQLTFEELTKLSRKSLQEWLEERGMHPEAYAYIKVLAAS
                     QTGLAEPRMIAASDFLGYMAMAPDIRMNLISGSVATVDKPGTIAIPQEMEKTLVEHGG
                     EVLRNAPVSKVIVEDGTVKGVKYKKDGEQHTLTADTVICTVPPKYAFQVLPKEAFPVE
                     WVSFMKEEHRGIGLLTGWVGTKRSIIGDLGIDKRSFIFMPGITSEEEGFIGVVDMVMC
                     EFDSWKDGEADRAPDGKTEYYFSTALTDNE"
BASE COUNT         1004 a          622 c          789 g          893 t
ORIGIN      
        1 gcggtgaatt atctgggtca cttcgtattg gtaaacaggt taatgccctt gatgaaacag
       61 gctcaggcag gtcgtattgt ccacgttggt agtaaatctg cctatattca ggcacctgcg
      121 gtgggtatag actttgacaa ccttcgaggt gaaggtgtat tcgattcgca ggaagcttac
      181 ggtcgatcca agttggccaa tgccttattt tcccttgaac tggccgcacg gttggaaggc
      241 tccagcgtta cttcaaatgt tattcaccct ggtttagtca aaaccaatat cgctcgcacc
      301 gcccctatag ttctacgcaa agcgtttgaa tggtttggcg acttcattgc gaaaacacct
      361 caagaggggg ccgccactca ggtttatgtg gctaccaatc ccgctctcaa gggagtcagc
      421 ggcgcctatt ttgaggactg taatgcggtt accgtcggtg gtgataacca catattcgat
      481 aagccaatgg cagaacacct gtgggccacc ggtgaagaga tggcccaggg ttatctgata
      541 actattgagt gggctgtagg tgaattggca aaacataaaa aagatgactg acataatgaa
      601 gtcactttta aaatatctat ttattatggt tctgtcttct ggtttgactg taattgcatt
      661 aaatcaattt actccaattg tcattaccat cccaaaaaag gataaacaag aaattaagat
      721 tgattttgat caagctcttg caaagaaaga tttaaagaga atagatagga aagctaaaaa
      781 tataattctt ctaattggtg acggcatggg aagcaaccaa gtaaccgctt atcgaattgc
      841 gaaaggaggc ccaaattata ttacagcttt tgataaattt cccttcacag gcttagttaa
      901 aacacatgca gtagacactt taatcacaga ttccgcttca tcagctacgg cttatagctc
      961 tgggattaaa actgttaatg gatacttagg agttgataag gataaacaaa tagtaggcaa
     1021 cataactgaa atattatatg aaaaaggata tgtcagtagt ctaattgcaa catctgaaat
     1081 tacccatgca acacccgctg catttgcagt tcacaatgaa tcaagagaca acactgatca
     1141 aatagctgaa gcaatattta agtctaataa ttttatttta ttaggaggcg gacgagattt
     1201 ctttctgcct aaaaatttag gaggtcaaag agaagataag cttgatattc ttagtctaat
     1261 aaaagagcaa gaacgttatt tgagttcaaa agaagatttc aataatttcc aatttgatcc
     1321 atcaaaaaga atttttggtt tgtttgcaag tgaagcgttg attagaaaag aaaatgaacc
     1381 aaatctttta gaaatgttta atttcacact agagcaatct gaaaagatgc ttgtaactaa
     1441 ttgtgctggt tttttcataa tgactgaagg ctctcaaata gattgggaag ggcatgaaaa
     1501 tgattttaat gaccaattta aagagatgga tgagttcaat caagtcgtcg accgcgcatt
     1561 agaatatgct aaattagata caaatacatt agtcatcgtc ctcgcagacc atgaaaccgg
     1621 aggtctgctt atagaaatgg atgattttag aaataaacct agtaacaaga tgaaaatttc
     1681 atggaacact gctgttgggt atggcacaca tacaggatcg atgatacctg tctatgctta
     1741 tggtcctggg gcagaaaatg taaccggagt cattgataac actgatattt tttttattat
     1801 gaaggaagcg ctaggtcttg atgaattaag tgatcgccaa tgtttataag aaaatatacc
     1861 cataaataac atatttaaca gattgttatc ggtggtgata accacatatt cgataagcca
     1921 atggcagagc gcctgtgggc taccggcgaa gagatagccc agggttatct gataactatt
     1981 gagtgggaat agagagaact caaaagaatt gtcggaaaaa tataataaag gaggagaaga
     2041 catgacgaca gagaattatg acgtcattgt catcggcgca ggtgtggctg gaatgactgc
     2101 tgcaagctta ttaggtcagg attgcgataa gaaagtcctc gtccttgaac gggcaccatt
     2161 tatcggtgga cgctgccttt cttatgttgg tgaaggtgac aaggtcgtag ccgatggggt
     2221 tgaaatggat gcccgagctt tcaagaaatc cctacccctt gctcattgtt acctcagtaa
     2281 atgtacgccg ggtattgaaa ccattttttc ggaaggtctt ctcgatggtc gaacctttga
     2341 ggcgggaggg catgggctct tctggggtaa caataaccgg gcagattgca tgctcaagca
     2401 tgttggcagg catgtggaaa tgccgttgaa ccgaggcctg ggattcatcg agtggcaagg
     2461 tctgaatgat gatggcacgg ttaaaccaac tgttgcccac caggtacaaa aaggacaaac
     2521 ctatccctgg atgagtgagg aaggtttcgc caagaccaga gatcagctca ctgatatgag
     2581 ccagctcaca tttgaagaac ttacaaaatt gtcgcgaaag tctttgcaag aatggcttga
     2641 agaacgcggt atgcaccctg aggcatacgc ctacattaag gttctagctg cctcccagac
     2701 tggcctggca gaaccgagaa tgatagccgc ctccgacttt cttggctata tggcaatggc
     2761 gccagatatc cgtatgaatt tgatctcggg ttcagttgca acggtcgata aacctggcac
     2821 gatcgcaata ccacaggaaa tggaaaaaac tctggtcgag catggcggtg aggtattgcg
     2881 caatgcgccg gtttccaagg tcattgttga agatggcacg gtcaagggag tgaaatacaa
     2941 aaaagatggc gaacaacata cgctgaccgc agatactgtt atctgtaccg ttcctcccaa
     3001 gtacgctttc caggtattgc caaaggaagc ctttcccgtg gagtgggtta gctttatgaa
     3061 agaagaacac aggggtattg gtctgctcac tggctgggtc ggtaccaaac gttccatcat
     3121 tggagatctt ggtatcgata agcgttcgtt tatctttatg cctggtatta ccagtgaaga
     3181 agaaggattt attggtgtcg ttgacatggt gatgtgtgag ttcgactcct ggaaggatgg
     3241 tgaggcagat cgggctcctg atggtaaaac tgaatattac ttctcgactg cactgactga
     3301 caacgaga
//
LOCUS       DASCTH010000014         2114 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000024785, whole genome shotgun sequence.
ACCESSION   DASCTH010000014 DASCTH010000000
VERSION     DASCTH010000014.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000024785"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="QGH35_00260"
     CDS             complement(<1..700)
                     /locus_tag="QGH35_00260"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HJL80999.1"
                     /translation="MVRAKIEKLALTFDDVLIRPNYSEVLPRDTDLATQLTSTIRLNI
                     PLISAAMDTVTEFQLAIAIAQEGGLGIIHKNFSIDDQSAQVNRVKKFESGVINDPITI
                     SPDKTIQEVLEITKKNNISGVPVVDKDETVGIVTNRDLRYQNQLDALVSSVMTPKEKL
                     ITVKADEKKEEVLRLLHHHRIEKVLVVDDNFKLKGMITAKDFQKASDFPLAAKDQNGA
                     LLVGAAVGVGEESYT"
     gene            760..>2114
                     /gene="xseA"
                     /locus_tag="QGH35_00265"
     CDS             760..>2114
                     /gene="xseA"
                     /locus_tag="QGH35_00265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HJL81000.1"
                     /translation="MLPSAMENIDHEHIYSISELNREVFNMLSSAFGVIWIEGEISNF
                     VKSAAGHAYFSLKDESSQIRCAMFRHRNQLVNIEIENGKQILAKAKVGLYEARGDYQL
                     IIEYLEEAGEGLLKQRFELLKNKLEKEGLFDRANKLSLPRFPQKLGIISSSSGAAIQD
                     IFNIIRRRFPSVDIFVYPTQVQGTQATKQICQAIETANDRNECELLILARGGGSLEDL
                     WCFNEEDVARAIFQSKIPIISGIGHETDLTIADMVADHRAPTPSGAAEIALPDSEEIQ
                     RQLAGIEKQLYSNMKLLLNEIKQQWLSFDLKLQQQHPVSIMEQYNQRFDLVLQRIKPI
                     PKEYISRISNTINVLNTRLFRSTPLPLSQEMKAETGNLRSRLMASIQETYKNIEQLLF
                     VSMAKLDGVSPLNTLGRGYAYVIDPKTKKHIRSIEQIKTGSDTKTRLKDGTFDAKISR
                     V"
BASE COUNT          642 a          449 c          397 g          626 t
ORIGIN      
        1 gtgtgtatga ttcttctcca acgccaactg cagctcctac caaaagggct ccattctgat
       61 cttttgccgc caaaggaaaa tcacttgctt tttgaaaatc ctttgcggtg atcatgcctt
      121 tgagtttgaa attatcatcc accacgagta ctttctcaat cctgtgatga tggagtagtc
      181 tcaaaacctc ttctttcttt tcatctgctt tgacggtaat taatttctct ttgggtgtca
      241 tgacagaact aactaaagcg tccaactgat tttgatatcg tagatcacga ttggtcacta
      301 tgcctaccgt ttcatcctta tcgacaactg gaacaccgga aatattattc ttcttagtga
      361 tttcaagtac ctcttggatc gttttatctg gtgaaatcgt gatgggatca ttaatgactc
      421 cactctcgaa tttcttaaca cggttaactt gagccgattg atcatcgata ctgaaatttt
      481 tatgaatgat gccaagtcca ccttcttggg caatggcgat tgctagctga aattccgtca
      541 ctgtatccat tgcggcagaa atcaatggaa tgtttagacg tattgttgac gtcaactggg
      601 tggccaaatc cgtatcgcgt gggagaactt ccgagtaatt cggcctgatt agaacatcat
      661 cgaatgttaa tgctaatttt tctatctttg ctcgaaccat gggcaatttt acactgccct
      721 aatgaattgg taaaccttag ataagattgt tttgtataat tgctaccctc tgctatggaa
      781 aatattgatc acgaacacat ctattccatc tctgaactca acagagaagt tttcaatatg
      841 ctctcttctg catttggtgt tatttggatc gaaggtgaaa tttccaactt tgtaaaatct
      901 gcggcaggac acgcctattt ttctttaaaa gatgaatcct ctcagatccg atgcgcgatg
      961 tttagacatc gtaaccaact cgttaatatt gaaattgaaa atggcaaaca aatactggcc
     1021 aaagccaaag tcggcctcta tgaggccaga ggtgactacc agttgattat tgaatatctt
     1081 gaggaagctg gagaaggatt attaaagcaa cgttttgaat tattaaaaaa caagctagaa
     1141 aaagaaggtc tctttgatag agccaacaaa ttatcactcc ctagatttcc acaaaaactt
     1201 ggcatcatca gttcttcctc aggagctgcc attcaagata tttttaatat cattagaaga
     1261 cgattcccta gtgttgatat ttttgtctac ccaacgcagg ttcaaggaac ccaagcaaca
     1321 aaacaaattt gccaagccat tgaaacagcc aatgatagaa atgaatgtga actcttaatc
     1381 cttgcacgtg gtggaggatc actggaggat ttatggtgtt tcaatgaaga agatgttgcc
     1441 cgggctattt ttcaatccaa aataccgatc atctcaggca ttggccatga aaccgattta
     1501 acaattgccg atatggttgc tgatcaccgg gcacccacac cttcgggtgc tgctgaaatc
     1561 gctttgcctg actcagagga aattcaaagg caattggctg ggattgaaaa gcaactctat
     1621 tccaatatga agctgctctt aaacgaaata aaacaacaat ggttgtcctt cgatttaaaa
     1681 ctacaacagc aacacccggt ttcgattatg gaacaataca accaaagatt tgatcttgta
     1741 ttgcagcgta ttaagccgat tccaaaggaa tacatctcaa gaatttccaa tacaattaat
     1801 gtcctcaaca cccgtttatt ccgatccact cccttgccct tatcacaaga aatgaaagct
     1861 gagacaggca atcttcgtag ccgactcatg gcttccatcc aagagaccta caaaaatata
     1921 gaacagctat tgtttgtttc catggctaaa ttggatggcg tttcacccct gaataccctc
     1981 ggtagaggct atgcttatgt tatagatcca aaaactaaaa aacacatcag gagcattgag
     2041 caaattaaaa ctgggtcaga tacaaaaact cgcttaaaag acggtacttt cgatgccaaa
     2101 attagtcgag ttga
//
LOCUS       DASCTH010000015         1661 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000027480, whole genome shotgun sequence.
ACCESSION   DASCTH010000015 DASCTH010000000
VERSION     DASCTH010000015.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000027480"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="QGH35_00270"
     CDS             complement(<1..498)
                     /locus_tag="QGH35_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012264.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HJL81001.1"
                     /translation="MNKSGRKNMQIHLWGLFFCLMLIQSLGVSLELNSETLGNANYQS
                     GKITFQQRCSACHTLADNSANLAGPNLWNMFNRSIGKAPGFNYSQGLRETDSSWTPNI
                     LASFLQKPETVFRNTTMRIPEPVPESLLNDLIAFIMVETDAPNKPNIETPVLDQLVDR
                     ELPLSE"
     gene            616..1503
                     /locus_tag="QGH35_00275"
     CDS             616..1503
                     /locus_tag="QGH35_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81002.1"
                     /translation="MLNFFKKKNPGQWLESPMHDNWYQSSAYFCSSFALITSVNEEGV
                     TSIGPYQLSFPFGVIHRREWIVISRKNSNTTNNLKRIKKCAMNFVEYDKRFVPNIVDL
                     GYPGQEPEEKMKDCLFELEESPTESYRQDPERPKVIKNAFQVFECNLNDNPEDFFYKG
                     NEYTDYLLLEIQKIYLREQWRKNLDLGDKTKIPNMPISYGFRNTNQFWFAQHGDPYWL
                     PTPEDKGAKHDAVMYIANRIDPEITFTEDACKLLTGIPKVFVKTALKGIIAEAKKEGI
                     TEIDESFVQMINEKRTGGS"
BASE COUNT          546 a          311 c          317 g          487 t
ORIGIN      
        1 ctctgacaat ggcaattctc gatcaaccaa ttgatccaat actggtgtct caatattggg
       61 tttatttgga gcatctgttt caaccataat gaatgcaatc agatcgttca aaaggctttc
      121 tggaaccggt tctggaatcc gcattgtggt attgcgaaag actgtttcgg gtttctgaag
      181 aaagcttgct aaaatatttg gtgtccagct tgagtctgtc tctctcaaac cttgtgaata
      241 attaaatccc ggtgcttttc ctattgagcg attgaacatg ttccataaat tcggtccagc
      301 taaattagcg ctgttatctg ccaatgtatg gcatgcacta cagcgctgtt gaaaagtgat
      361 ttttcctgat tggtaatttg catttcccaa agtctcggaa ttgagttcca atgaaacacc
      421 tagtgactgt atcaacatca aacaaaaaaa gagcccccaa agatggatct gcatattttt
      481 tctacccgat ttattcattt cattaatggt aaattagcat tattacttga aagataaaac
      541 ggttagaatc gtgattaata aacaaaatta ataaacaaac aagaagatcc gctaagtata
      601 agaaaaggta atgctatgtt aaattttttc aagaaaaaaa atcctgggca atggctcgaa
      661 agcccaatgc acgataattg gtatcaatcc agtgcatatt tttgtagttc ctttgcactc
      721 atcacatccg tcaatgaaga aggtgtaacc agtatcggtc cttatcaatt gtcctttcct
      781 tttggtgtga ttcatagaag agaatggatt gttatctcaa ggaagaactc taataccact
      841 aacaacttaa aacgcatcaa gaagtgtgcg atgaattttg tcgaatacga taaacgcttt
      901 gtgcccaata ttgttgattt aggatacccg ggccaagaac cagaggaaaa aatgaaagac
      961 tgtctctttg agctagaaga atcacccact gagtcctatc gtcaagatcc cgaacgacca
     1021 aaagtcatta aaaatgcttt tcaagttttt gaatgtaatt tgaatgacaa ccctgaagat
     1081 tttttctata aaggcaatga atacaccgac taccttcttt tggaaataca aaaaatttat
     1141 ctaagagaac agtggcggaa gaatctagac cttggggata aaactaaaat ccctaatatg
     1201 cctatcagtt atggatttag aaatactaac caattctggt ttgcccaaca tggtgaccct
     1261 tattggttac caacacccga agacaaggga gccaaacacg atgcagtgat gtatatcgcc
     1321 aacagaatcg accctgagat tactttcact gaggatgcgt gtaaattatt gacaggaatt
     1381 ccaaaagtat ttgtgaaaac tgctctcaaa ggcatcattg cagaagctaa gaaagaaggt
     1441 atcactgaga tagacgagag ttttgtgcag atgattaatg aaaaacgaac cggtgggtcc
     1501 taatttatgt cgcatcgtac cgatgaactt gaaaaattaa caggcattca atcaaccatt
     1561 cgtgaatggc gttaccatga attctccaga caaagcatag gcattattct gctcagtgtt
     1621 tttggattag ttgcaaaacc agagtttatg aactttgttc t
//
LOCUS       DASCTH010000016         5264 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000028123, whole genome shotgun sequence.
ACCESSION   DASCTH010000016 DASCTH010000000
VERSION     DASCTH010000016.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000028123"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="QGH35_00280"
     CDS             complement(<1..970)
                     /locus_tag="QGH35_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HJL81003.1"
                     /translation="MQYKTPVLLWLISSFLLSCHVPRTDLLPDQSSLRNTELGAIIGA
                     DNGKTHQWLGIPYADIPSSEYRWREAKAPLPWEDVRQTLEFGPFCTQLGSLINTTKRK
                     NWGEIVGSENCLSLNIWAPKPNPTEIENQNTVYPVMVWVHGGSNTAGSANFYNPTELA
                     HRHNLVVVTINYRLGPFGWFRHPSLTDQLGNKKDQSGNYGNLDTIAALQWVKNNIANF
                     GGDPNNITIFGESAGGYNVAALLSSTLAEGLFHKAVVQSGGIKPGDITHSENFISESL
                     PWKNYSSKELFNELLIAKGMAANKKEATEIQTNMLSKNVGSILRSAT"
     gene            1084..2880
                     /locus_tag="QGH35_00285"
     CDS             1084..2880
                     /locus_tag="QGH35_00285"
                     /inference="COORDINATES: protein motif:HMM:NF025418.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HJL81004.1"
                     /translation="MIAINYLKRMLLMTLLWVLVTFQMDHSEASPWAMPDNLMLRNDI
                     QLLVDSGVINIPITTWPLAWGDIAYNVSKTEKELTLLELSALQRIKEALYEEEMGGLS
                     GSMALKLAKNPERITWFNDSVAAKSEIEAETTYLGKRLALNIHVNKQSGETIFDDSYI
                     AMAIGDYTLSLGAKKNWWGPGWGGSLIQSTNTRPIPSISFERNFSDPFESKFLSWIGP
                     WDLSAMIGEMEQGTNFFGMRVGFRPKKNLELGFSTSALFCGENRSCGLSGLGKTLLDR
                     NITIDGGDLSVQKSGYQLAGIDFRSSHTFRDFPFAAYGQFIGEEPSDYMGLLGLETWG
                     SMNESEWLESYRVFVEGSSTHCQFYSSKTKCAYHNPLYPNGYWHKGGNIGHSSDGDSI
                     LLTLGVMFVGDNSQLIKSSLSVGQLNRNSSPLYMLTPKKTDYFNFGLGYEFDLFWFDI
                     PLGNFDVGLGLEYLKNFGKDPRVYIAWSNDADFSPKKVRDYSEYIELIEVDDDLEITS
                     GLLPDQIDEQPSFLALDTNLKIVQVMDLMDQVIRQRVPLLDNIKDRRLGIQTLDDKSI
                     IDKLDISLPTTVSEHALTELMIMIDQIMQNRN"
     gene            complement(2912..4159)
                     /locus_tag="QGH35_00290"
     CDS             complement(2912..4159)
                     /locus_tag="QGH35_00290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81005.1"
                     /translation="MKKIIVIIILLFSLALPGVSVAGSQSVLIQNATVMTASDLGILE
                     NTDLLIENGLIAAIGKQLTANDAEIIDASNMIVTPGLIAPHSQLGLVEIELEQQTRDD
                     STDHYSAGFSIANAFNPSSTLIPYNRSGGITSAIVVPASAKTNFSGMASVFNLDGKLL
                     TQPIAQDIAMVMNYGAESDSRASQILLLEDAFEKAVRFKRNKASILRGNYFDYGVYTI
                     RDLEAIMRVLDKDIPLLVRSNRAQDLLTMIAIAKKYRIKMVFYGAKEAWRIADVLARE
                     DIPVILDPMDNIPSSFDAIAARMDNAALLNAAGVTILITSQSTHNSYLSRQGAGNAAA
                     YGLPQDEALKALTINVADVFGLSQDRGSIAVGKSADLVIWDGNPLEVSSFATAMMIEG
                     HSISLISRSRRLRDKYLIELGLK"
     gene            complement(4156..>5264)
                     /locus_tag="QGH35_00295"
     CDS             complement(4156..>5264)
                     /locus_tag="QGH35_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81006.1"
                     /translation="APSLRASSDGNEATDPVTPHVWAEHSVWTQDPQFTLALKGGITT
                     FHVLPGSANLIGGRGVTLKNIRSVTVQGMKFPKAPYTLKMACGENPKRVYGNRKQEPS
                     TRMANVAGYRTAWIDAEYYLKKIDRFANKMEDNLLLDEADLDEDEDQERPPKRDLKME
                     TLAGVLKGEILIQNHCYRGEEMAVMLDIAKEFNYKVTTFHHAIEAYKVADLLADQGVC
                     AAMWADWWGFKHEAYDMVWANAAMVDQAQKGTGCAIIHSDSAIGTQHLNQEAAKSRAA
                     GIRAGLDISRGRAIKWITFNPAKALGISDQVGSLEVGKMADLVIWDGDPFSVFTKAEK
                     VFIDGHIKYDSQREEAFKRTDFDLGIIDPEDTRL"
BASE COUNT         1568 a         1101 c          994 g         1601 t
ORIGIN      
        1 tagttgctga tcttaaaatg ctccccacat tttttgatag catatttgtc tgtatctctg
       61 ttgcttcctt tttgtttgct gccattcctt tggcaatcaa tagttcatta aataattctt
      121 tggaactgta atttttccaa ggcaatgact cactgataaa attctcagaa tgggtgatgt
      181 caccaggctt gatccctcca ctttggacaa ctgctttatg aaataggcct tctgccaaag
      241 tcgatgacaa cagtgcagca acattataac caccagcaga ttcaccaaaa atagtgatgt
      301 tattgggatc accaccaaaa ttggcaatat tgttcttcac ccattgcagt gctgcgatgg
      361 tatctaagtt cccatagttt cctgattggt cttttttatt gcctaactga tcagtaagac
      421 taggatgcct gaaccatcca aaaggaccca atctataatt gatcgtaaca actaccaggt
      481 tatgtcgatg agccaactct gttggattgt aaaaattagc actcccagca gtattgctac
      541 ccccatgaac ccagaccata acaggataaa ccgtgttctg attttcaatc tctgtgggat
      601 taggttttgg agcccaaata ttgagactaa gacaattctc tgatccaaca atttctcccc
      661 aattttttct tttagtcgta ttaatcaaag aacccaattg ggtacaaaag ggtccaaatt
      721 ctaaagtttg acgaacatct tcccaaggaa gaggagcttt ggcctcacgc caccgatatt
      781 ctgatgaggg aatatcggca tagggaatac ctaaccattg atgggttttt ccattgtccg
      841 cgccaataat ggctcccagt tcagtatttc tgagcgatga ttgatcaggc aataaatcgg
      901 ttctcggtac atgacaagag agcaaaaatg atgaaatcaa ccataataaa actggtgttt
      961 tatactgcat aactatagtt ctaatgtatt aaaaaaagcc attttaaagt gctttaccat
     1021 ctcagaaaac atagatcgaa gataatatat tattacggcg caacatagta gaataccgtt
     1081 attgtgatag caataaatta tttaaaacgt atgcttttga tgactcttct ctgggtattg
     1141 gttacttttc aaatggacca ctcggaggca tcgccttggg ctatgccaga taatttaatg
     1201 ctcagaaacg atattcaact gctagttgat agcggtgtca tcaatatacc catcaccact
     1261 tggcctttgg catggggtga tattgcatac aatgtatcca aaacagaaaa agaattgact
     1321 ctattggaac tgtctgctct gcaacgaatc aaagaagctt tatacgaaga agagatggga
     1381 ggtttgagtg gttcaatggc tcttaaattg gcaaaaaacc ctgaaagaat cacttggttt
     1441 aatgactcag ttgctgccaa aagtgagatt gaagctgaaa cgacctatct tggaaaacgt
     1501 cttgcattga atattcatgt caataaacaa agtggcgaaa ccatttttga tgactcctat
     1561 atagcaatgg caatcggtga ttatacttta tccctaggtg ctaagaaaaa ttggtggggc
     1621 cctggatggg gtggtagttt aattcaatca accaatacca gaccgatacc gagtatctct
     1681 tttgagcgga atttttcaga cccttttgaa tccaagtttc tcagttggat aggaccttgg
     1741 gatctctctg caatgatagg tgagatggaa caaggtacaa attttttcgg catgcgagtg
     1801 ggatttcgac ctaagaaaaa tcttgaactg ggtttttcga caagtgctct tttttgtggc
     1861 gaaaatagat cttgtggact ttccggtttg ggtaaaacat tattggatcg aaacatcaca
     1921 attgatggtg gagatctatc tgtgcagaaa tctggatacc aattggctgg tattgatttt
     1981 agatcatcac atacattccg tgattttcca tttgctgctt acggacaatt catcggcgag
     2041 gaacctagtg actatatggg attacttggc ttggaaacct ggggatctat gaatgaatct
     2101 gaatggttag aaagctacag agtatttgtt gaaggctcct caacccactg tcagttctac
     2161 agttcaaaaa ctaaatgtgc ctaccacaac cccttatatc caaatggtta ctggcataaa
     2221 gggggcaata tcggacactc atcagatggt gactcaatat tactaacact cggagtaatg
     2281 tttgttggag ataattctca attgattaaa tcatcgctct ctgtgggtca gttgaataga
     2341 aattccagtc ccctgtacat gctgacacca aaaaagactg attatttcaa ttttggtctt
     2401 ggttatgaat ttgatctatt ctggtttgac atcccgcttg gtaattttga tgtgggactc
     2461 ggtttggaat atttaaaaaa tttcggcaaa gacccaagag tttatattgc ttggtccaat
     2521 gatgcagatt tcagtccgaa aaaagttagg gattacagcg agtatattga acttattgaa
     2581 gtcgatgacg atctggagat aaccagtgga ttattgccgg atcaaattga cgagcaacct
     2641 agctttttag ctctggatac caatttaaaa attgttcaag tgatggattt gatggatcaa
     2701 gtgatcaggc aaagagtgcc tctcttagat aacatcaaag acagacgatt aggaatacaa
     2761 acacttgatg acaaatcaat catcgataag ctcgatatat ccctaccaac aacagtcagt
     2821 gaacacgctt taacagaatt gatgattatg attgatcaaa tcatgcagaa cagaaactaa
     2881 gcagctgcga tttactgctg ctttaaatcc cctatttcaa accaagttct attaaatact
     2941 tgtctcgtaa acgcctagat cgagatatta aactgatgga gtgtccttca atcatcatag
     3001 cagttgcaaa agatgacact tcgagaggat taccatccca aataaccaaa tctgcagatt
     3061 tccctactgc aatgcttccc ctatcttgag acaagccaaa aacatcagca acattaatgg
     3121 ttaatgcttt gagggcttca tcctgaggca aaccgtatgc tgctgcattc cctgctcctt
     3181 gcctgctaag ataagaattg tgagtacttt gcgaggtaat taagattgta acaccagctg
     3241 cattaagtag tgctgcatta tccatccgag cagcaatagc atcaaaagaa ctcggaatat
     3301 tatccatagg atccaatatt accggaatat cttctcgagc cagaacatca gcaattcgcc
     3361 aagcttcttt agctccataa aataccatct tgatcctata ttttttagca atcgctatca
     3421 tggtcaataa atcttgtgcc ctattggacc taactaataa aggtatatcc ttatcaagga
     3481 ctctcataat tgcctcaaga tcccttattg tataaacgcc atagtcaaaa taattgcctc
     3541 ttaaaataga agctttattg cgtttaaaac gaacggcttt ctcaaaagca tcttctagta
     3601 gtagaatctg agaggcccta gaatctgatt cagcaccata attcatcacc atggcaatgt
     3661 cttgagcgat aggttgagtc aataatttgc cgtcgagatt aaaaaccgaa gccatacctg
     3721 aaaagtttgt ttttgcagat gcaggtacaa caattgctga agtgattcca ccagaacgat
     3781 tgtaaggaat cagagtagag gatggattaa aggcattggc tatactaaat cctgctgaat
     3841 aatgatcagt agaatcgtct ctggtttgct gctccaactc aatctccact aatcccagtt
     3901 gactatgcgg tgcaattaat cctggtgtca caatcatgtt tgatgcatca attatttctg
     3961 catcattggc ggttaactgc tttccaattg cagcaattaa accattttca atcagtaaat
     4021 cggtattctc cagaatccct aaatccgaag cggtcatgac ggttgcgttt tgaattaata
     4081 ctgattggga tccagctaca cttactcctg gaagtgctag actgaataag aggattatga
     4141 tcacaattat ttttttcata atctagtatc ctctggatca atgatcccta aatcgaaatc
     4201 tgttcttttg aatgcttcct ctctttgaga gtcatatttt atgtggccat caatgaatac
     4261 cttttctgct ttcgtaaaaa cactaaatgg atccccatcc caaatcacca gatctgccat
     4321 tttacctact tcaagactac cgacctgatc ggatattcca agagcttttg ccggattgaa
     4381 tgttatccac ttaatggctc taccccttga aatatccaaa ccggcacgaa tgccagctgc
     4441 tcttgactta gcagcttctt gatttagatg ttgagtacca atggcagagt ctgaatgaat
     4501 aattgcacat ccagtaccct tttgagcttg gtctaccatg gcggcatttg cccaaaccat
     4561 atcgtaggcc tcatgcttga atccccacca atccgcccac attgcagcac aaacaccttg
     4621 atctgctaac agatcagcaa ctttgtaagc ttcaatggca tggtgaaaag tggttacttt
     4681 gtaattaaat tcttttgcaa tatcaagcat aactgccatt tcttcgcctc gataacaatg
     4741 attctgaatc agaatttcac ctttcaaaac tcccgcaagt gtttccattt ttaaatcacg
     4801 tttaggaggc ctttcttgat cctcatcctc atcaagatca gcttcgtcaa gcaataaatt
     4861 atcctccatc ttatttgcaa aacgatcgat ctttttcaaa taatactcgg catcaatcca
     4921 agccgttcta tatcctgcaa catttgccat gcgcgtggat ggttcttgtt ttctatttcc
     4981 atagactcgt tttggatttt caccacaagc cattttcaga gtgtagggag cttttgggaa
     5041 cttcattccc tgaaccgtga cgcttctgat attctttaag gtgactcctc ttccaccaat
     5101 caaatttgcc gaacctggta aaacgtgaaa tgtggttatt ccacctttca gtgccaaagt
     5161 aaactgagga tcttgtgtcc aaacagaatg ctcagcccaa acatgcggag taaccgggtc
     5221 tgttgcttca ttcccatctg aactcgctct cagtgatggt gcgg
//
LOCUS       DASCTH010000017         3823 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000028893, whole genome shotgun sequence.
ACCESSION   DASCTH010000017 DASCTH010000000
VERSION     DASCTH010000017.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000028893"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="QGH35_00300"
     CDS             complement(<1..737)
                     /locus_tag="QGH35_00300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81007.1"
                     /translation="MSENNTIQTYPSNKRAWYAVIILTIAYIVSFLDRQILALVVQDV
                     KTDLDLSDTQVSLLLGFAFALFYTTMGIPIGRLADKKSRRAIIATGITIWCLMTAACG
                     LARNYTQLFLARVGVGVGEATLSPSGLSMISDYFPAEKRGKALGFFNMGVSVGSGIAF
                     IGGGQIIAYVSDAPPIILPYIGEIFAWQALFLLVGLPGLIVAVLMRTVKEPSRKDKII
                     IRGQDGQQTDEISVKQTIDFLLARKKT"
     gene            complement(755..1411)
                     /locus_tag="QGH35_00305"
     CDS             complement(755..1411)
                     /locus_tag="QGH35_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024852.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HJL81008.1"
                     /translation="MTTVILFGATGATGLALSKLLLKKSYILYTAARKEKDREYLSAL
                     GANAFLADALNANQVINAMSNITNEATVISLVGGSSNEQGTRSDFLGNKYIIESAVDL
                     EFKRLIFITSIGSGESKPHLPDVLKPILGPITDEKTKAENILRNSGLDFTIIRPGQLT
                     NDSATGDGLLTEECILGRMSREDLALMIIKCIEDKTTIGKIYSVLDANKKIDLSWMEK
                     "
     gene            complement(1424..2851)
                     /locus_tag="QGH35_00310"
     CDS             complement(1424..2851)
                     /locus_tag="QGH35_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013739.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJL81009.1"
                     /translation="MKTLINRRRTIQLLGAAVAVPGELAFGRSGNQDAQVIVIGAGVS
                     GLNTALILEEQGLDVLILEGSNRIGGRVYTLDDKKHKPNAGANELSAESHPRTVDMAK
                     RLGLAFIPWLGPGTGTGFPAMSFYINGKTVAMEQWATSPTNKIMGKTKSLPPIAQANA
                     FFPKQSPLEHLGSWLEPEAAYLDTSYYDYLQQQNIDPEALRIIQERHTSKDLREVSTL
                     WQLRLQRLFSFREVNTLFGIEGGTSRLTDGMTTLLKRNIDLNTKVTGIRVKDEGVEIK
                     DSTGRTYKAQYAVCTTPLTMLREITIEPTLPELHAAAVEQIPYDENISVFLDIKEPFW
                     EKDGLPPIMWAEGTGTWVKMTRDPEGHGYYWISISGNRTKKWQDKNDDIIVQDLIDEL
                     AKIRPSSKGKVEPIAVQNWHTHPWTKGHGAYLSPGQIKKFSDILTKPFGRLYFAGTHT
                     ARLSTGMEGAMEAGERAALDILLNL"
     gene            3108..>3823
                     /locus_tag="QGH35_00315"
     CDS             3108..>3823
                     /locus_tag="QGH35_00315"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HJL81010.1"
                     /translation="MMFTNVWYVAESSDKIKDQPVGVTMLGRDFVLFRDQSGKPVCLS
                     GVCPHRGADLSQGKCEPDGTVGCPFHAWRFDSNGRCTKIPSNKDPVGEIPDAARVDSY
                     PTEEKYGYIWVFLGDDLENAEPIFDIPEYKDSAWSSVTHAVTWNCNQHWAKQLDLDHV
                     HLPIVHGIGFGGDNPVRPPDHTVEYRNDGGFETKISGKPDITKGKWLELRDTRSEVSS
                     KFKYFVPGFTSRGKVAVGGV"
BASE COUNT         1012 a          823 c          830 g         1158 t
ORIGIN      
        1 taagttttct ttcttgctaa aagaaaatca atcgtttgtt ttacggaaat ttcatcggtc
       61 tgctgaccgt cttgtcccct gattattatt ttgtcttttc ttgaaggttc tttaacggtt
      121 ctcatcagca ctgcaacaat taaccctggg agacccacca acagaaatag agcttgccag
      181 gcgaaaattt cacctatgta tggaaggatg attggaggcg catcgctgac ataggctata
      241 atttgccccc caccgatgaa cgctatgcca gatccaacag agacacccat attgaaaaat
      301 cccaaggcct tgccccgttt ctcagcgggg aagtaatcac tgatcattga tagtccactg
      361 ggacttagag tcgcttctcc aacgcctaca ccaacgcgag ctaggaacaa ctgagtgtaa
      421 ttcctagcca atccacaggc tgccgtcatc agacaccaga ttgttatgcc tgtggcaata
      481 attgctcttc tgcttttttt atctgccaat cggccaatgg gtatgcccat ggttgtataa
      541 aacagtgcaa acgcaaatcc caacaataaa ctaacttgag tatcagacag atccaagtct
      601 gtttttacat cttgcactac caaggcaagt atttgacgat ctagaaatga aacaatatag
      661 gcaatggtta gaataataac ggcataccaa gctcttttat ttgaagggta ggtttggatc
      721 gtattgtttt cagacatctg ggttcaatta gttattattt ttccatccag cttaaatcaa
      781 tttttttatt agcatccaag actgaatata ttttgccaat tgttgtttta tcttcgatgc
      841 attttatgat catgagtgcc agatcttcac gggacattct tcccagaatg cactcttctg
      901 tcaagagacc atcacccgtg gcagagtcat tggtgagttg acccggtcta atgattgtaa
      961 aatccaaacc agagtttcta agaatattct ctgcctttgt tttttcgtca gtgatgggac
     1021 ctagtattgg ttttagaaca tctggcaaat gaggcttgct ttctcctgaa ccaatgctag
     1081 tgatgaaaat gagtctttta aattcaaggt caactgccga ttcaataata tatttattgc
     1141 ccaagaaatc agatctggtt ccttgttcat tactcgatcc acctactaag ctaatgaccg
     1201 tggcctcatt ggtgatgtta ctcatagcat taatgacttg attggcattt aatgcatctg
     1261 ctaagaaagc attagcccct aaggcactca aatactctct atctttttcc tttcgagcag
     1321 ctgtgtataa aatataactc ttttttagaa gtagttttga tagagccaag ccggtggctc
     1381 ctgttgcccc gaacagaata acagttgtca ttcaatcaac ttgctagaga ttcagtaaaa
     1441 tgtcaagagc agcacgttca ccagcctcca tggctccttc cattcctgtt gataggcgag
     1501 cggtatgagt tcctgcaaaa tagagtcgtc cgaatggttt cgttaaaata tcactaaatt
     1561 tcttgatttg ccctggactt agataggctc catggccttt ggtccaggga tgggtgtgcc
     1621 aattttgtac tgctatgggc tctacttttc ctttggaact ggggcgaatt ttagccaact
     1681 catctatgag atcttggacg atgatgtcat cgtttttgtc ttgccatttt tttgttcgat
     1741 tgccagagat tgatatccaa tagtaaccgt gaccctctgg gtctctggtc attttcaccc
     1801 aagtaccggt tccttcggcc cacattatcg gagggagccc atctttttcc cagaacggtt
     1861 ctttgatatc aagaaaaacg gaaatatttt catcataggg tatttgctct actgcagctg
     1921 catggagttc tggcagggta ggttcgatgg ttatttctcg aagcatagtt aatggagtgg
     1981 tacacacggc atactgagcc ttataggttc ttcccgtgct gtctttaatt tcaacacctt
     2041 catctttgac tcgaatacca gttacttttg tgtttaagtc aatgttgcgt ttgagtagtg
     2101 ttgtcatgcc gtcggtcaat cgactcgtac ctccctcaat gccaaacaag gtattcactt
     2161 cacgaaagga aaataatctt tgtaaacgta gttgccatag ggtagagact tcacgaagat
     2221 ctttactggt gtgtcgctct tggataattc tcaatgcctc tggatcaata ttttgttgtt
     2281 gtaggtagtc gtagtaggaa gtatccaaat aggcagcttc gggttccaac cacgagccta
     2341 aatgttcaag cggcgattgt ttagggaaaa atgcattggc ctgcgctatg ggtggcaatg
     2401 acttggtctt acccataatc ttgtttgtcg gtgaagttgc ccattgttcc atcgctacag
     2461 tttttccatt gatgtagaac gacatggctg gaaaaccagt gccagttccc ggtcctaacc
     2521 aagggataaa agcaagcccg agacgtttag ccatatccac tgtccgaggg tgagattcag
     2581 cgcttaactc attcgctccc gcattcggct tatgtttctt atcatccaga gtgtaaacac
     2641 gaccgccaat acgattactg ccttccagga tcagaacgtc taaaccttgt tcttctaaaa
     2701 ttaaagccgt gttgagccca gatacccctg cgccgatgac gataacttgg gcatcttgat
     2761 ttcctgatct accaaaagct agttctccag gaacagctac tgctgcgcct aacaattgta
     2821 ttgtacgtct acggttaata agtgttttca ttccatctcc taaaaaaata gtacaaacag
     2881 ctacatctta gatgatgtag agactagaca ttaacatagg tctttgtcta gtaattgata
     2941 tagatcagtt tttatatgat ttagatcaat ttcaaaacca atatcaatct atatcattga
     3001 taagcaatgg atattgatat tattcttgaa gccggtttag tagtctgcaa actaaaatct
     3061 tgctctcgac ttagggcgga agataggaag attaggaggg acgagttatg atgtttacta
     3121 atgtttggta tgtagctgaa tcgagcgata aaatcaagga tcagcctgtg ggagtgacaa
     3181 tgttgggccg ggatttcgtg ctatttcgcg atcagtccgg taaaccagtt tgtctcagtg
     3241 gcgtttgtcc acaccgcggg gctgacttgt ctcagggaaa gtgtgagcct gatggcacgg
     3301 tcggttgtcc ttttcatgcg tggcgttttg acagtaacgg cagatgcaca aagattcctt
     3361 ccaacaaaga tccggtcggt gagattcccg atgcagcacg tgttgattct tacccaacgg
     3421 aagaaaaata tggttacatc tgggtattct tgggcgatga tctagaaaat gcagagccaa
     3481 tcttcgacat accagaatac aaagattcgg catggtccag tgtgacacat gccgtgacct
     3541 ggaactgtaa tcagcattgg gcaaagcagc ttgatctcga tcacgtgcat ttaccgatcg
     3601 tacatggtat cggcttcggt ggtgacaacc cagttagacc cccggatcat acggttgaat
     3661 atcgtaatga cggcggcttt gaaaccaaga tctctggcaa gccggatatc acaaagggca
     3721 agtggttgga gctacgcgat acacgcagtg aggtgtcttc gaaattcaag tatttcgtgc
     3781 ctggatttac atcacgtggc aaagttgctg tcggcggggt aga
//
LOCUS       DASCTH010000018         2440 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000028991, whole genome shotgun sequence.
ACCESSION   DASCTH010000018 DASCTH010000000
VERSION     DASCTH010000018.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000028991"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="QGH35_00320"
     CDS             complement(<1..509)
                     /locus_tag="QGH35_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HJL81011.1"
                     /translation="MAGHSKWANIQHRKGRQDKKRGKLFTKLIREITVAARIGGSDLN
                     SNPRLRSAVDKAKDQSMPKENIDRAISKGTGEGEGFNLEEVTYEGYGPGGVAILIECM
                     TDNKNRTVAEVRYALSKFGGNLGTEGSVAYLFQQVGLISLSGVKDPDRIMEIAIDSGA
                     EDIEYDENGS"
     gene            611..1225
                     /locus_tag="QGH35_00325"
     CDS             611..1225
                     /locus_tag="QGH35_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81012.1"
                     /translation="MRRFSVILPVLFSVLLSSCTITSYQKIDSAVGYWDESIDQSTDT
                     FKIGFDGAEWNKNSLVEQEKVIDYALLRSAEIALDNGFRYFLISENQSYTEKKTTKET
                     ETSNSETFRHRAIFTTVNYIDLEEKMRHNNLIQDGEFKGYAVYNAALVFYTIKTKYNL
                     SNNKLVQNFESGMPAYRMPGTSRGMTETIDAVPHNPINSPLPDQ"
     gene            complement(1345..2367)
                     /locus_tag="QGH35_00330"
     CDS             complement(1345..2367)
                     /locus_tag="QGH35_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HJL81013.1"
                     /translation="MTRINQRWLLNKRPEGMPEDDCWKWEEVDVPELKDGEILIKSLY
                     LSIDPYMRGRMNDAMSYTGPVQIGDVMVGESVGIVVESKSDNFKPDDYVCVHGGWQSY
                     VAVRGDGPELFKADPNIVPLSTYLGTVGMPGRTAYFGLTRLGKPKEGETVVVSAASGA
                     VGTVVGQIAKILNCRAVGIAGGEVKCSYVTDELGFDACIDYKGGNLEQDLKQACPDGI
                     DVYFENVGGEVTQAVAKLLNPGSRVPICGFVSKYNSQDIMNEETPFDVFGTMRPKPKH
                     RFFVVTEWFDHWADATKELSDWIKEGKIKYRETVTDGLINAPQALRDVLSGKNFGKQL
                     VKIADE"
BASE COUNT          721 a          547 c          394 g          778 t
ORIGIN      
        1 gatccgttct cgtcatattc aatatcttct gccccagaat caattgctat ttccatgatt
       61 ctatctggat ccttaactcc ggataatgaa atcaaaccaa cttgctgaaa aagataagca
      121 acggatcctt cggttcctaa attacctcca aacttagata atgcatatct cacttcagct
      181 acggttctat ttttattatc agtcatgcac tcaattaaaa ttgccactcc acctggtcca
      241 tagccttcat aagtcacttc ttctaaatta aaaccttccc cttctcctgt tcctttggaa
      301 atcgctcgat caatattctc cttaggcatg ctttgatctt ttgccttatc aacagctgac
      361 cgtaatctgg gattggaatt taagtcactg cctccaattc ttgcggcaac agtgatttca
      421 cgtattagtt tggtaaaaag cttacctctt tttttatctt ggcggccctt cctgtgttgg
      481 atgttagccc atttactatg tccagccatt taagtcctat gatgcaatca ttaataatgg
      541 aacctgatca attatacaat aaaccgttta ttatagtgtt caggtaaaat gtttttgtat
      601 aatagatcct atgagaagat ttagtgtcat tttacccgtt ttattttcag ttttgctctc
      661 ttcatgcaca attacgagct atcaaaaaat agactcagca gttggatatt gggatgaaag
      721 cattgatcaa tctactgaca cttttaaaat aggatttgat ggtgctgaat ggaataaaaa
      781 ttccttagtt gagcaagaaa aagtcattga ttatgcattg cttcgatctg ctgaaatagc
      841 attagataat ggatttagat attttttaat cagcgagaac cagtcctaca cagaaaagaa
      901 aaccaccaaa gagacagaaa catctaatag tgaaaccttt cgtcacagag caatttttac
      961 gaccgtcaat tatattgacc ttgaagaaaa aatgcgccac aacaatttga tccaagatgg
     1021 ggagtttaaa ggttatgctg tttacaatgc tgctttagtg ttctacacca ttaaaacaaa
     1081 atacaactta tccaataata aattagtaca gaattttgaa tctgggatgc cagcatatag
     1141 gatgccagga acttcacgag gaatgactga aacgatagat gcggttccac ataatccaat
     1201 taatagtcca cttccagacc agtaatctac taaattctaa tgcttgagtt gatggcttag
     1261 ctacgcctgg attattgttt cttgtttgct gagttttgtt tatgagaaag cttccagtgt
     1321 tctctccaca aaaaaaatct agatttattc atcggcaatc ttaaccaact gttttccgaa
     1381 attcttacca ctcaatacat ctcttagggc ctgaggcgca ttgatgagtc catcagttac
     1441 tgtttctcgg tatttaatct tgccttcttt aatccagtct gagagttctt tggtggcatc
     1501 tgcccaatga tcaaaccatt ccgtcactac gaaaaatctg tgcttaggtt ttgggcgcat
     1561 cgtaccaaat acatcgaaag gggtttcttc gttcataata tcttgcgagt tgtatttgga
     1621 aacaaaacca catatgggaa ctctactacc agggtttaat aacttggcaa cagcctgagt
     1681 aacctcacct ccaacattct cgaaatacac atctattcca tccggacagg cttgcttgag
     1741 atcttgttct agatttccac ctttgtaatc gatacaagca tcaaatccaa gctcatcagt
     1801 tacataggaa cactttactt ctcctccagc aatgcccaca gctcgacaat tgagtatttt
     1861 ggcaatttgt cccacaaccg ttcctactgc tccagaagca gcagaaacaa caactgtttc
     1921 tccctcttta ggtttaccca atcgggttaa accaaaataa gccgttctgc ctggcatacc
     1981 gacagttcct aagtaagttg ataacggaac aatattagga tcagccttaa aaagctcagg
     2041 accatcacct ctaacagcaa cgtagctctg ccaacctcca tggacacaga cgtagtcatc
     2101 aggtttaaag ttgtctgatt tgctctccac cactatccca acactttctc ccaccataac
     2161 atcaccaatc tgtactggtc ctgtataaga catggcatca ttcatgcgtc ctcgcatgta
     2221 agggtcaata gaaagatata gagatttaat tagaatttca ccgtctttca attctggaac
     2281 atctacttcc tcccatttcc agcaatcatc ctcaggcatc ccttctggtc ttttgtttaa
     2341 taaccacctt tgatttattc tggtcattat tgtctcccta acattaattt tgatcagcca
     2401 acaaatatta agaattattt cgaacttgtg gcagtttatt
//
LOCUS       DASCTH010000019         3620 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000030938, whole genome shotgun sequence.
ACCESSION   DASCTH010000019 DASCTH010000000
VERSION     DASCTH010000019.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000030938"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="QGH35_00335"
     CDS             complement(<1..1466)
                     /locus_tag="QGH35_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJL81014.1"
                     /translation="MKKYDAIVIGAGHNGLTNAAYLAKSGLKVLVVEKNDYIGGASVS
                     RELHEGWTYSNCSYVCSLLRPEIFRDLNLQKYGLQVLPYGGSVTFMQNGDIFGSYSDD
                     DLARREIGRFSKRDADAYVRFARDVSKQCRFIKPMLLRTPPDPTSFKPKDLGELIYLF
                     KRLYSLGEQTIYDTIRFYTMSVAEYLDEYFESDVIKAALSGSGIIGTGLGPYSPGTAY
                     VLLHHYMGEVDGSIGSWGFARGGMGSVAKALADAFKDYDGELLTDAEVSQIIVKGGRA
                     KGVVLVNGDEHYAKNIVSNLDVKRTYQKLFDPKDLSEKVLKQVDAFKIRGSSGKVNIA
                     LDGMPEFPAMGKDNPLICSDMHFIDSTERIERAYDDWKNGRWSRDPYCDTLLPTMTDP
                     TMAPPGKHYMSVFVQYAPIKLSTGEWTDQDRDDFGSTVIDQIAEYSPNFKDLIVHAEV
                     RSPQDIENEIGLTAGNIFQGELTMDQMFFNRPFPGYAQ"
     gene            complement(1480..2784)
                     /locus_tag="QGH35_00340"
     CDS             complement(1480..2784)
                     /locus_tag="QGH35_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJL81015.1"
                     /translation="MTRSLKKSNEHFQKAIKKLPLGVTSNFRYWGDEDTIYVDRASGC
                     RIRDIDGNEYIDYRLAYGPYILGYADPRVDQAAREGMEIGGVFALSTELEYEVAQKIS
                     SMVPSAELVRFSNSGTEAVLAALRVARAYSNRDEYIMLEGGYHGVSDSALWDIDLDDW
                     DPTTGEPEITSSSKGVPERLSKMVHLVPMNDANRLEDLLKKKHNEIGALLIEPILGNC
                     CSISADQDYMTSVRELCTKYDVVLIIDEVKTGFRVAKGGVQELYGIEADLCTYAKSIA
                     NGYPISALCGKEEMMRLIKYGGVLHGGTFTAHSVAISAANKTLEIIESTDALDTVCDY
                     GLQLRKGLSKILSDYDTPHSFTGHPSMSGLFLSENPPTNARDFRSSNYPLYEKLAEEL
                     LEYGVLCEFDPREPWFICESHAKDALKPTLDAFEAAAEVTLK"
     gene            3028..>3620
                     /locus_tag="QGH35_00345"
     CDS             3028..>3620
                     /locus_tag="QGH35_00345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL81016.1"
                     /translation="MHIDLPENAKKWAKIADGFLQQTIPWEIEAEMNEGKLPEDIDKQ
                     HRQTAIDLGLSAMGIPKSYGGKELSSLEQVVVWEVLGRGTNALTWCFSEPQSWMVDAC
                     NEEQIKTYILPLMDGSRHECYAITESESGSELNVSSTAKKVKGGYVVNGEKWFVTGAN
                     HADFFFIQAAVMDGEERLGDALFFLDMDTEGVELVRTP"
BASE COUNT         1116 a          769 c          695 g         1040 t
ORIGIN      
        1 tattgagcat aaccagggaa gggtctatta aaaaacattt gatccatggt caactcacct
       61 tgaaaaatat ttccagccgt taggccaatc tcattttcaa tgtcctgtgg gctgcgtact
      121 tcagcatgca caataagatc tttaaaattg ggactgtact cagcaatttg atcaatgact
      181 gtagatccaa aatcatcccg gtcctgatct gtccactccc ctgtagaaag cttaattgga
      241 gcatattgca caaagacaga catgtagtgt tttcccggtg gagccattgt tggatcggtc
      301 atggtaggaa gtagagtgtc gcaataaggg tctcttgacc agcgtccgtt tttccagtcg
      361 tcataagcac gttcaatgcg ttctgtggaa tcgataaaat gcatatcaga acagatcaaa
      421 ggattgtcct tacccatagc tggaaattcc ggcatcccat caagagcaat atttacttta
      481 cctgaagaac ccctgatttt aaatgcatcg acttgtttga gaactttttc actcaaatct
      541 ttgggatcaa ataacttttg gtaggttctt tttacatcta gatttgaaac gatatttttt
      601 gcataatgct catctccatt gactaaaact acacccttag cccgtccccc tttgacaata
      661 atttgagaga cttctgcatc agttaacaat tcaccatcat agtctttaaa tgcatcggcc
      721 aacgctttcg ctacagaacc catgccaccc cttgcaaatc cccaagagcc aatactaccg
      781 tcgacttctc ccatgtaatg atgcaataac acgtaggctg ttcctggaga ataaggaccc
      841 aatccggtgc cgatgatacc actgccagaa agagctgctt tgatcacgtc agattcaaaa
      901 tactcatcca agtattcagc aacactcatg gtgtaaaagc gaatggtgtc ataaatggtt
      961 tgctcaccaa gtgagtacaa acgtttgaaa agataaatca gctctccaag atctttgggt
     1021 ttaaatgatg tcggatctgg tggggttctc agaagcattg gtttgataaa ccgacactgt
     1081 ttggaaacat ctctggcaaa acgaacataa gcatcagcat cacgtttaga aaatcgtcct
     1141 atttctctcc tagctaaatc atcatcagag taacttccaa aaatgtcacc attttgcatg
     1201 aaggtaacac tgcctccgta agggagaact tggagaccat atttttgtaa attcagatca
     1261 cgaaaaattt caggtctcaa aagactgcat acgtaggaac aattagaata ggtccaaccc
     1321 tcatgaagtt ctcggctgac agacgctccg ccaatatagt catttttctc aacaaccaag
     1381 acttttaaac ctgattttgc taaataagct gcgttggtca acccgttgtg acctgcacct
     1441 atgactatgg catcatattt tttcatcaag tgtgcctgtt cattttagag taacctcagc
     1501 tgcagcttca aacgcatcaa gagtgggttt caatgcatct tttgcatgcg attcacaaat
     1561 aaaccacggt tcacgcggat cgaactcgca taatacccca tattcgagta attcttcagc
     1621 aagtttttca tacagtgggt aatttgaact acgaaaatcc cgagcattgg taggtggatt
     1681 ctctgataaa aataatccag acatcgaagg atgtcccgtg aaactatggg gagtatcgta
     1741 atcactcaat atcttactaa gtccttttct caattgaaga ccgtaatcac aaacggtatc
     1801 aagagcatcg gttgattcaa taatctctag tgttttgttt gccgcagaaa ttgccactga
     1861 atgagcggtg aatgtcccgc catgcagcac ccctccatat ttaattaacc gcatcatttc
     1921 ttcttttccg cacaaagctg aaatggggta gccgttggcg attgatttag cataagtgca
     1981 tagatcagcc tcaataccat agagctcctg aacaccgcct tttgctactc taaatccagt
     2041 tttaacttcg tcaataatca acacaacatc gtattttgtg cataactctc gtaccgaagt
     2101 catatagtct tggtcagctg aaatagaaca acagttacct aaaataggtt caatcaataa
     2161 agcaccaatt tcattgtgtt tctttttaag cagatcttcc agacgattgg catcattcat
     2221 aggaacaagg tgcaccatct tcgataatct ttcaggaacc cctttgctag aagaagtaat
     2281 ttcaggttca cctgtagttg ggtcccagtc atccaaatca atgtcccaga gtgccgaatc
     2341 tgaaacaccg tgataaccgc cctcaagcat aatgtattca tcgcgattgc tatatgctct
     2401 agcgactctc aatgccgcca ataccgcttc agtacccgaa tttgaaaaac gaaccaattc
     2461 agcagagggc accatggatg agatcttttg tgcgacctca tactctaact cagtggaaag
     2521 tgcaaagacg cctcctatct ccatcccttc tctagcggct tgatcaaccc taggatctgc
     2581 ataacctaaa atatatggac cataagctaa acggtagtct atatattcat tgccatcaat
     2641 gtctcgaatt cgacaaccag aagcacgatc tacataaatg gtgtcttcat caccccaata
     2701 cctaaaattg gaagtcactc caagtggtag cttcttaatt gctttttgaa aatgctcgtt
     2761 actttttttt aatgatctcg tcactatgta atcaatttga ttgaatttaa ctgaaagatt
     2821 ggttgtatgg aatatagcag aattcctact tcactttaga aaagtgtaga tcagaattaa
     2881 ttacgtgaat aaataatatt aaatggagaa tctaataagt atgtcatcct cgtatcttaa
     2941 tagaatcttg ctaactttgg aagaataata ctatatctat atcattaaga gtaatatgag
     3001 cttagtaatt tctttacgga gaagcacatg catattgatt taccagagaa tgcaaaaaaa
     3061 tgggcaaaga ttgcagatgg atttttgcaa caaaccatac cttgggaaat tgaagcagaa
     3121 atgaatgaag gaaaacttcc agaggacatt gataaacaac atcgacaaac ggcaatagac
     3181 ttaggattaa gtgctatggg tattccaaag tcttacgggg gtaaagaatt aagtagcttg
     3241 gagcaagtag ttgtttggga agtgttggga cggggtacca atgctttaac ctggtgtttt
     3301 tcagagccac aatcctggat ggtagatgcc tgtaatgagg agcaaattaa aacctatatt
     3361 ttacccttga tggacggaag caggcatgaa tgttatgcca tcaccgaatc ggaatcaggc
     3421 tctgaactaa atgtttcatc aacagcaaag aaggttaaag gtggttatgt agttaatgga
     3481 gaaaaatggt ttgtaaccgg tgccaatcac gccgattttt tcttcattca agcagccgtc
     3541 atggatggag aagaaagact gggtgatgct ctgttctttc tagacatgga tacagaagga
     3601 gttgaacttg taaggacacc
//
LOCUS       DASCTH010000020         2940 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000031271, whole genome shotgun sequence.
ACCESSION   DASCTH010000020 DASCTH010000000
VERSION     DASCTH010000020.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000031271"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="QGH35_00350"
     CDS             complement(<1..1548)
                     /locus_tag="QGH35_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HJL81017.1"
                     /translation="MPNKNTRKKTKESKYDSRNIKVLKGLDAVRKRPGMYIGDTDDGT
                     GLHHMVFEVVDNSVDEALAGYCSEILVTIHQDDSITVKDDGRGIPVDKHKEEKVSAAE
                     VIMTFLHAGGKFDDDSYKVSGGLHGVGVSVVNALSETLNLRIHKDGYNYEQNYTNGVP
                     DKPLKKLGKTEKSGTTISFKPSLKTFSQASFHYEILAKRLRELSFLNSGLVIKLHDER
                     NNKNESFLFKGGIKAFIKHLNKLKVAHHDEIIYFNKTKDKVSVEIAIQWNQGYRENMY
                     CFANTIRQRDGGTHLAGFRGALTRSINTYIEKEQPKSKTTVTGDDCREGMTAVLSIKH
                     PDPKFSSQTKDKLVSSEVKGIVESVMNKCLTEFLLEKPKEAKAITSKVIDSARAREAA
                     RKAREMTRKGALEISGLPGKLADCQEKNPELCEIFLVEGDSAGGSAKQGRDRKTQAIL
                     PLKGKILNVQKAQKDKILSSVEIGTLITALGCGIREEFDLEKLRYHKVIIMTDADVDG
                     AHIRTLLL"
     gene            complement(1808..2905)
                     /gene="dnaN"
                     /locus_tag="QGH35_00355"
     CDS             complement(1808..2905)
                     /gene="dnaN"
                     /locus_tag="QGH35_00355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HJL81018.1"
                     /translation="MKILIKREKLKKAISSVVGVVDRKQTMPILGHILLENYENSLKL
                     TATNLEVQISSMTQAVEMDSFESTAVSGRKLYEIIRSLDSKEVELNSTKGQLIIKTKY
                     SSYKISTLPGNDFPIFDNSETSEVFKMNQEKLLSLFNKTHFSMAQQDVRFYLNGLLLE
                     TEPNKIKAIATDGHRLAASEMSVEKKQLNKTASIVPRKAIQELIRLLDSNEDVKAEIS
                     PNSIRLTFNDLSFITKLIDGKFPDYNRVIPTNTEINIQLNSLALKPSLQRVSILANEK
                     FKGIRLETTGNSLNLSSENPEQEEAYETISLQEKVKSIVVGFNVSYLIDAVSSCEGNA
                     VCLSLNDENSSALITSPEDKDTKYVVMPMRL"
BASE COUNT          728 a          561 c          528 g         1123 t
ORIGIN      
        1 caagagaagg gttctgatat gggcaccgtc aacatcagcg tcagtcataa ttatcacttt
       61 atgatatcta agcttttcaa ggtcaaactc ttcccttatt ccacagccca gtgctgtaat
      121 caatgtacca atttctacag atgaaagaat tttgtctttt tgtgcttttt gaacattgag
      181 tatcttgccc ttcagcggaa gaattgcttg tgtttttcta tccctgcctt gtttagcaga
      241 gcctcctgcg gagtcaccct ctacaagaaa tatttcacac agctcagggt ttttttcttg
      301 acagtctgca agctttccag ggagccctga gatttctaat gctccctttc tggtcatttc
      361 tcttgctttt ctggcagcct ctcttgctct agctgaatca atgactttac tcgttatagc
      421 ttttgcttcc ttgggttttt ccaacaaaaa ctctgtcagg catttgttca ttacagactc
      481 aacaattccc tttacttctg acgagacaag cttgtctttg gtttgtgatg agaactttgg
      541 atcagggtgt ttgatagaca atacggccgt catcccctct ctgcagtcat ctcctgtcac
      601 ggtagttttt gacttcggct gttccttctc aatgtaagtg tttatagatc ttgtaagcgc
      661 cccacgaaag cctgctaagt gtgttccacc gtctctttgt ctaatcgtgt ttgcaaaaca
      721 gtacatattt tctctatagc cctggttcca ctgaatggca atttcaactg agactttatc
      781 tttggttttg ttgaaataaa taatttcatc gtggtgggct actttaagtt tgtttaggtg
      841 ttttataaac gcttttatgc ctcctttaaa aaggaagctt tcgtttttat tgtttcgttc
      901 gtcatgaagt ttaatgacca atccagaatt tagaaacgac agttctcgaa gtctcttagc
      961 taatatttca taatgaaatg acgcttgaga aaaagttttt aggcttggct taaagctaat
     1021 tgttgtgcca cttttttctg ttttgccaag ttttttaagt ggcttgtctg gcacgccatt
     1081 agtgtagttt tgttcgtagt tatagccgtc tttgtggatt cttaggttaa gcgtctctga
     1141 cagtgcgtta accacagaaa cacctactcc gtgaaggccg cctgaaacct tgtaagagtc
     1201 gtcatcaaac tttccaccag cgtgtaaaaa agtcattatt acttctgctg ctgatacttt
     1261 ttcctctttg tgcttgtcaa caggaatgcc tctcccgtca tctttaacgg tgattgagtc
     1321 atcttgatgt atggttacta atatctcaga gcaataacct gcaagggctt cgtctactga
     1381 gttgtctaca acctcaaaca ccatatggtg aagtcctgtg ccatcatcgg tatcaccaat
     1441 atacattcct ggtctttttc ttaccgcatc aagacccttt aaaactttaa tgtttctaga
     1501 gtcgtatttt gattctttag ttttttttct ggtgtttttg ttgggcatct ttatatttaa
     1561 acagttctat taaagcgtgt ttgtattata acaaaactct tgtttttttg ctttggaaaa
     1621 agcactaaat ataggtcttt tttttgtttt tatgcaatat acaagctatt gatttatatg
     1681 ccttttctta gatttaatca atatgctctt ttttatctta aaaacaatgc ttgtttgtgc
     1741 ttttgttcca cgtggaacac ccctgtctgt tgcttttttt tctgtcattt gtttgagtga
     1801 aaagctttta aagtcgcatt ggcattacaa catacttggt gtctttgtct tctggtgaag
     1861 taatcaacgc gctgctgttc tcatcgttca ggcttaaaca aacagcgttt ccttcacaag
     1921 agctcactgc atcaattaag tagctaacat taaagcctac gacaatactt tttactttct
     1981 cttgtagcga tatcgtttca taagcctctt cttgttcagg attttctgat gaaaggttaa
     2041 gcgagtttcc tgtagtttca agtctgatgc ctttgaactt ttcatttgcc aagatggaca
     2101 ctctttgaag tgagggcttt agtgccagac tgttaagctg gatattaatt tcagtgtttg
     2161 ttggaatgac cctattatag tcagggaatt tcccatctat tagttttgta ataaaactta
     2221 aatcattaaa ggtaagccga atgctgtttg ggcttatttc agcttttacg tcttcattgc
     2281 tatccaatag tctaataagt tcttggattg cttttcttgg aacaatgctt gcagttttat
     2341 ttagttgttt tttttcaaca ctcatttcac ttgcggcaag cctgtggcca tcggttgcaa
     2401 ttgcttttat tttattaggc tctgtttcta gtaaaagacc gtttaaataa aacctaacgt
     2461 cttgctgtgc cattgaaaaa tgggttttgt taaagagaga caaaagtttc tcttggttca
     2521 ttttaaacac ttcagatgtc tctgagttat caaatattgg aaagtcgttt ccagggagtg
     2581 tagatatttt ataagaactg tatttagttt ttataatgag ctgtcctttg gttgaattaa
     2641 gctcaacctc tttagagtcg agagaacgta taatttcata aagttttctc ccagaaacag
     2701 cagtgctttc aaaagaatcc atctcaactg cttgggtcat tgatgaaatt tgaacttcta
     2761 aatttgtagc agttagtttt agagagttct cgtagttttc taaaagtata tgaccaagaa
     2821 taggcattgt ttgctttcta tcgacaacac caacaactga gcttattgct ttttttaatt
     2881 tctctctttt aattaatatt ttcattttat ttatttattt attaatatta gggctgttag
//
LOCUS       DASCTH010000021         4028 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000031618, whole genome shotgun sequence.
ACCESSION   DASCTH010000021 DASCTH010000000
VERSION     DASCTH010000021.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000031618"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="QGH35_00360"
     CDS             <1..585
                     /locus_tag="QGH35_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024742.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81019.1"
                     /translation="FQHGLGRGLKDLGTYFALSSIVIILIQPVWVRLAKQGGKAKLYA
                     IAAIGFVAIALSWFWTGTGTSMTSILVRSALSGIFVGGLLLMGQSLLPDTIHYDWQQN
                     GLRREGIFAGIYTTVEKLSFALGGVTAGFILQFIGYQSSLSGESVIQTASAVSGIYFL
                     SVVAPAILMLLSCIPLMYYPLKESDLTTDTNHAT"
     gene            575..1675
                     /locus_tag="QGH35_00365"
     CDS             575..1675
                     /locus_tag="QGH35_00365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJL81020.1"
                     /translation="MPRNEKHELQIIPTQQEAARQRFVLALKQDIGGRLRPQLKQVIP
                     SKIIDQQDSNSIATILEKSTPRKIWGRLARTAQELMWQGVEDPLLRKEKDLIKKCTAL
                     REKPIAGGSLELDENLIIPEAMIKVPVHLQPGGYCLDRHQQDILAGALYEAGGALFSQ
                     GQSINVDESKAELVVRFLHQKLPHFRPSHILDLACSAGGSSTPYARLFPKADVHAIDI
                     APGLLRYAHARAESIGVAVHFHQRDVCAPGFDDNSFDLVVSHNAMHEMSPITIKAMMR
                     ESYRLLRPGGVAVHQDVPLRNAQLNAYAQADYAWDYLYNNEPYWNSFAELNLSSTMQD
                     AGFEKTVQFDRVPQLGGSMDWDLFWAIKKMET"
     gene            1675..1971
                     /locus_tag="QGH35_00370"
     CDS             1675..1971
                     /locus_tag="QGH35_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81021.1"
                     /translation="MKNKDPDLEKVLAFTDNLPAFTGDQAVDKLYDMLMRTLQELAVT
                     REEVRTLQLLLKENGMDPDKIFDALSDQPEEVEARLKAHKKMIIRVIGDLERRK"
     gene            2075..2521
                     /locus_tag="QGH35_00375"
     CDS             2075..2521
                     /locus_tag="QGH35_00375"
                     /inference="COORDINATES: protein motif:HMM:NF019284.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81022.1"
                     /translation="MVNRRERFKRLYPKRLKNAVWNIRSIGKLSARTNYIYEDAEVDE
                     ILSTLVFELKAVITKFGLDPPDVPSFSTTIRKRNHYTDGKKDGYWESFHENGQLSERG
                     SYKDGLKVGLWEHYHNNGQLWVRGEYTKDGTRDASWEYFDKNGKKF"
     gene            2579..2971
                     /locus_tag="QGH35_00380"
     CDS             2579..2971
                     /locus_tag="QGH35_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81023.1"
                     /translation="MNLIGKKNILFGLYFFVATVGLGLFLAKVGENSEIPDQTLKMLK
                     QAHVHGNLESLLNVIFGFLIVNYGAQSETLSKVASISLILGTLCHSGVFYLYGLGIES
                     AMSISPVGALLVLVSIVLMIPITYKGVR"
     gene            3012..3332
                     /locus_tag="QGH35_00385"
     CDS             3012..3332
                     /locus_tag="QGH35_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81024.1"
                     /translation="MKELLLISALLLFSADSFAQEKYCWVNGDNFHDFKVAINKDCKT
                     GDLLWVVTNGHSLDEFTLEQTIGQYCDFESEIILRKRNDGSLYLQCILVDEKSRKRRT
                     NPTD"
     gene            complement(3360..3450)
                     /locus_tag="QGH35_00390"
     tRNA            complement(3360..3450)
                     /locus_tag="QGH35_00390"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3414..3416),aa:Ser,seq:gct)
     gene            complement(3460..>4028)
                     /locus_tag="QGH35_00395"
     CDS             complement(3460..>4028)
                     /locus_tag="QGH35_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014342.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HJL81025.1"
                     /translation="GTRRIEAITGDEALKWAEETEYLVEKLADMLQTGRDGIEKKVQT
                     LVNNNRQMNLEIKDLKAQIIRQPSGDTANEIETINSISLLIKELAEDTEPSVMRSAID
                     QIKQDIGSGVIILASKSSKGKVRVAIGVTEDLVEKVQANILANEMSKVLGGKGGGRAN
                     FANAGGSDTENLLKSFDLAKDWIKNLEF"
BASE COUNT         1247 a          752 c          809 g         1220 t
ORIGIN      
        1 ttccaacatg ggttaggcag aggattaaaa gatttaggca cttattttgc cctgagctct
       61 attgtgatta tcctgattca gccagtttgg gtacgactag ctaagcaggg tggtaaagca
      121 aaactttatg ccatcgcggc cattggtttt gtagcaattg ccttgtcatg gttctggaca
      181 ggaaccggta ctagtatgac ttccatccta gtgcgttctg cactctctgg tatatttgta
      241 ggcggcttgt tattaatggg gcagtcactt ttaccggaca ctattcatta cgactggcaa
      301 cagaatggtt tacgacgtga aggtatcttt gcaggtattt atactacggt agaaaaactc
      361 tcatttgcac tgggaggtgt aacagcagga tttattttgc agtttattgg ttatcaatcg
      421 tcactaagcg gtgaatcggt aatccaaaca gcatcagccg tatcaggtat ttacttttta
      481 tcagtagtag ccccagctat tctgatgtta ctcagttgca ttccacttat gtattacccg
      541 ctgaaggaaa gtgacttaac cacggataca aaccatgcca cgtaacgaaa aacatgaatt
      601 acagataata ccaacacaac aggaagctgc ccgccagcgt tttgtactag cactaaagca
      661 ggatattgga ggacgactgc gacctcagct taaacaagtg attccaagta aaatcataga
      721 tcaacaagat agcaattcta ttgccactat ccttgaaaaa tcaacaccaa gaaaaatctg
      781 gggccgcctg gcacggactg cacaggagtt aatgtggcaa ggagtagaag atccactgct
      841 tcgcaaagaa aaagatttga taaaaaaatg cactgcattg agagaaaaac cgattgctgg
      901 aggtagtctt gaactggatg agaacctaat aatccctgag gcaatgatca aagtgccagt
      961 acatcttcag ccaggtggtt attgtctaga tcgtcaccag caggacattt tagctggagc
     1021 attatacgaa gctggtggcg cgctcttctc tcaaggccaa tcaatcaatg tagatgaaag
     1081 taaagcagaa ttagttgtgc gatttttaca tcaaaaattg ccccatttta gaccttccca
     1141 tattttagat ttagcgtgct cagcaggtgg atcttcaact ccttatgcgc gtttatttcc
     1201 taaagccgat gtccatgcca tagatatcgc accaggatta ttacgatatg cacatgctcg
     1261 tgctgaatcg ataggtgttg cggttcattt tcaccaaagg gatgtgtgtg ctccaggttt
     1321 tgatgacaat agctttgatc ttgttgtttc tcacaatgcc atgcatgaaa tgtcgccaat
     1381 aacaattaaa gctatgatga gagaaagtta tcgcttattg cgaccgggtg gtgtcgccgt
     1441 tcaccaggat gttccattac gaaatgctca gcttaatgct tacgctcagg ctgactatgc
     1501 ttgggactac ttatacaata atgagcccta ctggaatagc tttgctgaac taaatttaag
     1561 ttccactatg caagatgccg gtttcgaaaa aactgtgcag tttgatagag tgccacaact
     1621 tggtggtagc atggattggg atttattctg ggctattaaa aagatggaaa cataatgaaa
     1681 aataaagacc cagacttaga aaaagtatta gcattcacag ataatctacc agctttcaca
     1741 ggagaccaag cagttgataa attatatgac atgctaatgc gaaccttgca ggaacttgca
     1801 gttacccgtg aagaagttcg cacactacaa ctgctgctta aagaaaacgg tatggatccc
     1861 gataaaatat ttgatgcact ttcagatcag ccagaagaag tcgaagctcg tctaaaagcc
     1921 cataaaaaaa tgattataag ggtaatcgga gacttggaaa gaagaaaata atcttaatca
     1981 ttggacaact actctttgct tctatttgat attatatata tttcattatt atataatata
     2041 ttatatatat ttagttatta tataattaat aaatatggtg aatcgaagag aaagatttaa
     2101 aaggctgtat cctaaaagac taaaaaatgc tgtatggaat attcgctcaa tcggcaaatt
     2161 atcagcacgt accaattaca tttatgagga tgcagaagtc gatgagattt tatcaacact
     2221 tgtatttgaa cttaaagcag ttattactaa atttggattg gatcctccag atgtgccttc
     2281 gttttcaacg acaattagaa aaagaaacca ctacacagat ggtaaaaaag acggctattg
     2341 ggaatcattt catgaaaacg gtcaattaag cgagagagga agttataaag acggattaaa
     2401 ggttggtctt tgggagcatt atcataacaa tggtcaatta tgggtcaggg gtgaatatac
     2461 caaagatggt acacgggatg ctagttggga gtattttgat aagaatggaa agaagtttta
     2521 gttatactga attgaggatg ttttgtgtta agtagagtaa taaaattagg agaacactat
     2581 gaatttaatt ggtaagaaaa atattttatt tggtctgtat ttttttgtag caaccgtagg
     2641 tctcgggctc tttctagcaa aagtaggaga gaactcagaa ataccagatc aaacacttaa
     2701 aatgctcaaa caagcccatg ttcatggtaa tttagaatcc ttattaaatg ttattttcgg
     2761 attcttaatt gttaactatg gagcacaatc tgaaactcta tctaaggtcg catcgatctc
     2821 actgatcctt ggaactttat gtcattcagg tgtgttttat ctttatgggt taggaataga
     2881 aagtgctatg agcatctctc cagttggagc tttattggta ctcgtcagca tagttttaat
     2941 gataccaatt acctacaaag gtgtgcgtta ataaagttgc tctcccgagt aaatgcatac
     3001 ttctagacca aatgaaagaa ctacttctca tatcagcact actgctattc tctgctgact
     3061 cttttgctca ggaaaaatat tgttgggtaa atggagacaa tttccatgat tttaaagtag
     3121 caatcaataa ggattgtaaa acaggagatt tattatgggt tgttaccaac ggtcatagtt
     3181 tggatgaatt tacccttgaa caaacaatag gacaatactg tgattttgag tctgagatta
     3241 tccttagaaa aagaaacgac ggtagtttat accttcaatg tatcctggta gatgagaaat
     3301 cgaggaaaag aagaacaaat ccaactgatt gacaatgatg gctctgaaaa ataagcctct
     3361 ggcggagagg gagggattcg aaccctcgat acgctattaa cgtatactcc cttagcaggg
     3421 gagcgctttc agccactcag ccacctctcc taaataagac taaaactcca ggtttttgat
     3481 ccaatctttg gctagatcga aggattttaa taaattttct gtatctgagc cacctgcatt
     3541 tgcaaaatta gctcgacctc ctcctttgcc accaagtact tttgacattt catttgccaa
     3601 aatattagct tgaacttttt ctaccaagtc ctcggttaca ccgattgcga ctctaacttt
     3661 acctttggaa ctcttactag ccagaataat cactcctgaa cctatatctt gtttaatttg
     3721 gtcgattgca gaacgcataa ccgaaggttc agtatcttct gccaattctt taatgagcaa
     3781 agatatgctg ttgattgttt cgatttcatt tgctgtatct ccagacggct gacgaatgat
     3841 ttgagccttc aaatccttaa tttcaagatt catttgtcga ttgttattca ctaaagtttg
     3901 aacttttttc tctattccat cccgtccggt ttgcaacatg tcagcaagtt tctcaaccaa
     3961 gtattctgtt tcttcagccc atttcaatgc ttcatctcct gttatagctt caattctcct
     4021 agttcctg
//
LOCUS       DASCTH010000022         6100 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000033839, whole genome shotgun sequence.
ACCESSION   DASCTH010000022 DASCTH010000000
VERSION     DASCTH010000022.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000033839"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="QGH35_00400"
     CDS             complement(<1..373)
                     /locus_tag="QGH35_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HJL81026.1"
                     /translation="MSDQIKSVRGMPVILPKDIKKWQFVEATARSLLQLYSYNEIRTP
                     VVEKSELFQRTIGKETDIVAKEMYTFQDRKEQSLSLRPEATAGIVRAGIEAGLFYNQS
                     QKFWSIGPMYRYERPQKGRYRQ"
     gene            complement(413..811)
                     /locus_tag="QGH35_00405"
     CDS             complement(413..811)
                     /locus_tag="QGH35_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024805.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJL81027.1"
                     /translation="MRTHDDNDLAAKQNELFVGASLKKARESKKLSIEDVAKGLYVVP
                     SIVVALEEEAFDKIGPVVFIKGHLKNYATFLGLPAEEMLKVLSRSGQMKEQKIVVPGI
                     ADHMIALKIISYASAFILVITIVGIYISHH"
     gene            complement(822..1916)
                     /gene="rlmN"
                     /locus_tag="QGH35_00410"
     CDS             complement(822..1916)
                     /gene="rlmN"
                     /locus_tag="QGH35_00410"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011398230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HJL81028.1"
                     /translation="MNSNLNNLIGKPFDDLVIFFQTIGERKYRAKQLMDWLYHRGVFD
                     FDAMTDLNKKLREYLNQNCSLEIAAATQTQTSSDGTIKWVIDQGQAQAIETVYIPEKS
                     RSTLCVSSQIGCLIDCPFCATGHQGFNRNLSSAEIIGQVLLAKQALKQELKQDHAITN
                     IVFMGMGEPLANFSETIKAIKILTDPNGFNLSRRRVTISTSGLVPQIMKLADYVNVSL
                     AVSLHAPVNKLRDELVPINRRHPIEALLDACWFYAKNGRLKTVTFEYTLLKGINDQPQ
                     HARQLCKLLKDRPAKVNLIPFNPFPGVAYQPTPVYQINEFRNILLESGIMTMTRTTRG
                     DDIKAACGQLSGMVNNLAKQSLGQKLKTIN"
     gene            complement(1941..2345)
                     /gene="ndk"
                     /locus_tag="QGH35_00415"
     CDS             complement(1941..2345)
                     /gene="ndk"
                     /locus_tag="QGH35_00415"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HJL81029.1"
                     /translation="MSIERTLSIIKPDGVRQKIIGEINKRFEDAGLAIVNAKMIQLSK
                     SDAEGFYAIHKDKPFFNDLVAYMTSGLVMVQVLEGENAIQLNRKIMGATNPAEAAAGT
                     IRSDFASSIEENVVHGSDGPETAKAEIEYFFG"
     gene            complement(2367..2690)
                     /locus_tag="QGH35_00420"
     CDS             complement(2367..2690)
                     /locus_tag="QGH35_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HJL81030.1"
                     /translation="MNIKITDKALGRFKKNLDQRSDAIGLRISITASGCNGYSYVLDY
                     ATEVEPTDEVCEISNISILVDQESLQYIKGTEIDFEEEGFNQVFKFKNPNATGECGCG
                     ESFSI"
     gene            complement(2702..3067)
                     /locus_tag="QGH35_00425"
     CDS             complement(2702..3067)
                     /locus_tag="QGH35_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HJL81031.1"
                     /translation="MTENLTRFYFDQEDHLFNWEDDQHTYEAKGSATNSQGMKIDLYF
                     KVEDGRIVDAKYNVSGCPVLIAISAFYIENVIGKTLDGCHDFNAIGIHESIEIPNHKK
                     DRMILLENAVDDCLGQIKE"
     gene            complement(3079..4248)
                     /locus_tag="QGH35_00430"
     CDS             complement(3079..4248)
                     /locus_tag="QGH35_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012488.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJL81032.1"
                     /translation="MKEIIKQIYLDYASTTPLAREVVSEMEAVMSGQSFGNPSSVDHL
                     FGKQAYEAVENARLDIASLINADAGEIIWTSGATEANNLAILGLARFKKNGFKQQVIS
                     SFAEHKSVIEPCLQVKKEGFELTLLNPNANGSVDINELEGAINEKTLMVSLMHVNNEV
                     GSINDIKSIGNLCRQNNVFFHVDAAQGIGKLPIDVKDYKIDLMSLNAHKVYGPKGIGA
                     LFINHESVGRVEPILIGGGQERSLRPGTLATHQIVGMARAYKIAQEKMSSDHRHLRQC
                     RELFIDTCKDISKLSVNGHSENVFPGILSVSIDGLYAESIIYGMKIAAVSKGSACNSD
                     DDEPSHVLKSMGLSQSEINGTVRFSFGRDTEIKDVEYACRLYRDSVDHLHRLSGD"
     gene            complement(4245..4964)
                     /locus_tag="QGH35_00435"
     CDS             complement(4245..4964)
                     /locus_tag="QGH35_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HJL81033.1"
                     /translation="MNPQVKFVLFEPSHPGNVGAAARAIKTMGFDLLCLINPKEHPHP
                     EARARSSGALDVLLDAEVCDNLNDAIENCGLVVGTTSRTRRISAPTSTIRDIASSIIN
                     KAKHKPVAILFGPEKTGLMNEQIDCCNQLIHIPSSQLYRSLNLAMAIQIIAYELHFAW
                     QALPSEIETRNLASNSEMELFYEHLNQVLLETGFLNPRNPRQLMRRLRTLFNRAQLDE
                     NEMNIMRGILASYKDKSDTST"
     gene            5069..5878
                     /locus_tag="QGH35_00440"
     CDS             5069..5878
                     /locus_tag="QGH35_00440"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HJL81034.1"
                     /translation="MQAFLNTAIEAARKAGDIAQMNFGRINESDIKTKSYNEFVTFVD
                     KQAEAAIKEIIQSRYPDHGMIAEESGENTNNKQDFVWIVDPIDGTTNYIHSFPVYAVS
                     IALQIKGVLEVGVIYDPSRQELFTALKGQGSRLDERRIRVSKQSTLEGALLGTGFPYR
                     QNKDWLDIYLEIFSEFSQKAAGIRRPGAAALDLAYIASGRLDGFWEFGLNSWDIAAGV
                     LLIQESGGLCENLIPDQDMIGSGNLFAGNPKIFDQMKQIISKYGPQLTQLS"
     gene            complement(5898..>6100)
                     /locus_tag="QGH35_00445"
     CDS             complement(5898..>6100)
                     /locus_tag="QGH35_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HJL81035.1"
                     /translation="TTLLVLLALYLIGGKSLEGFSLALIIGVIVGTYSSIYVATTSIF
                     LLDVSTSDLLQVNKEQLDDGMP"
BASE COUNT         1806 a         1289 c         1057 g         1948 t
ORIGIN      
        1 attgacggta tcgccctttt tgcggtcttt catacctata catcggtcct atagaccaga
       61 atttttggct ttgattgtaa aataatcctg cttcaatgcc tgctctcacg atgccagcag
      121 ttgcctcagg cctcagtgac agagattgct cttttcggtc ttggaatgta tacatttctt
      181 ttgcaacaat atcggtttcc ttacctatcg ttctttgaaa caattcactt ttttcaacca
      241 cgggtgttct gatttcatta tatgaatata actggagaag acttcttgcg gtggcttcca
      301 caaattgcca cttcttaatg tctttaggta ggataactgg cattcctctg acagatttta
      361 tctggtcaga catttaattc tcctctagtt tcttatccac agaaagcaac catcaatgat
      421 gcgatatata gatacctaca atggttataa ctagtatgaa tgcacttgca taggaaataa
      481 tttttaaggc aatcatatgg tcggcgattc ctggtacaac aattttctgt tctttcattt
      541 gtccactcct tgatagtact tttagcatct cttcagcagg gagtcctaga aaagttgcat
      601 agttttttaa atgacctttg ataaaaacaa ccggtccaat tttatcaaag gcttcttcct
      661 ccaaagcaac aacaatggaa ggaaccacat aaagtccctt tgcaacatcc tcgattgata
      721 gcttcttgga ttcccgtgct ttctttaagg aagcgccaac aaacaattca ttctgtttgg
      781 ctgcgaggtc attgtcatca tgtgttctca taaattataa actaattgat cgtttttagt
      841 ttttgtccca atgattgctt tgctaaatta ttcaccattc cagacaattg accgcaggct
      901 gcttttatat catccccacg agttgttcta gtcatggtca ttattccaga ctccaagagt
      961 atattcctaa actcattaat ttgatagaca ggagtgggtt ggtaggcaac cccaggaaat
     1021 ggattgaatg gaatcaaatt gaccttagca ggccgatctt tcaaaagctt acacaattgc
     1081 ctggcatgct gtggctgatc attgatgcct ttcaacaaag tatactcaaa tgtgacagtt
     1141 tttagtcgcc catttttcgc ataaaaccaa caggcatcca acaatgcttc aataggatga
     1201 cgtctattaa tcggtaccag ctcatctctc aatttgttaa ctggagcatg caatgaaact
     1261 gctaatgaaa cattcacata atctgcaagt ttcataatct gtggaacaag acccgaagtg
     1321 ctgattgtca ctcttcttcg cgataaattg aaaccattgg gatctgttaa aatttttatc
     1381 gctttaatag tttcagaaaa gtttgctaaa ggctctccca tacccataaa aacaatattg
     1441 gtaatggcat ggtcttgctt tagttcttgt ttcaaagctt gttttgctaa caatacttgg
     1501 ccaataattt cagccgatga taaatttcga ttaaaccctt ggtgacctgt cgcacagaat
     1561 ggacagtcga ttaaacagcc gatttggctt gatacgcata gtgtgcttct gctcttctct
     1621 ggaatataca ctgtttcaat ggcttgtgct tgcccttggt caatgaccca tttaatcgtt
     1681 ccatcagagg atgtttgagt ttgagtggct gctgcaatct cgagtgaaca attctgattt
     1741 aaatactctc tcagtttttt attcaaatct gtcatggcat caaaatcaaa aacaccacgg
     1801 tgatataacc aatccattag ctgctttgct ctgtattttc tctcgccaat ggtttgaaaa
     1861 aaaataacga gatcatcaaa aggtttgccg attaagttat tgaggttaga gttcaagggg
     1921 tgccctcatt ctgtaaagac tcaaccgaaa aaatactcta tttcagcctt agccgtctca
     1981 ggtccatctg aaccatggac cacgttttcc tctatactcg atgcaaaatc acttctgatg
     2041 gttccagctg ctgcttctgc agggtttgtt gctcccatga tttttcgatt gagctgtatg
     2101 gcattctcgc cttctaaaac ttgtaccatc accaagcctg aagtcatgta tgccacaaga
     2161 tcattaaaaa agggtttgtc cttgtgaatg gcataaaatc cctctgcatc agatttggag
     2221 agttgaatca ttttggcatt gacaatagcc aagcctgcgt cttcaaaacg cttgtttatt
     2281 tcaccgatga ttttttgtct aacgccatcc ggtttgataa tggaaagagt tctttcaatg
     2341 ctcatcagaa ttccttatac aaagatctaa atactgaagc tttcaccaca accacattca
     2401 ccggttgcgt ttggattttt aaatttaaaa acctgattaa aaccctcttc ctcaaaatca
     2461 atttccgtcc ctttgatgta ctgcaaactt tcctgatcaa ccaggattga aatatttgat
     2521 atctcacaca cctcatctgt tggttcaacc tcagttgcat aatccaaaac ataggaatag
     2581 ccgttacaac cactggctgt aatactaatc ctaagaccta tggcatctga cctttgatct
     2641 aaattttttt taaagcgtcc tagtgcttta tccgtaatct taatattcat agaaaattca
     2701 cctactcctt aatctgacct aaacaatcat caacggcatt ctccaaaagg atcatcctat
     2761 cttttttatg gttaggaatc tctatactct catggatgcc aatcgcatta aagtcatgac
     2821 aaccatccaa tgttttaccg attacattct cgatataaaa ggcagaaata gcaattaaaa
     2881 ctggacaccc actaacgttg tatttagcgt cgacaattct accgtcttca accttaaaat
     2941 aaagatcaat tttcattcct tgggagtttg tggcactgcc tttagcttcg taagtgtgtt
     3001 gatcatcctc ccagttaaag agatgatctt cttggtcaaa ataaaatctt gttaaattct
     3061 cagtcatata tttctatttt agtcaccgct caatcgatgc aggtgatcca cagagtctct
     3121 atataagcga caagcgtatt ctacatcttt gatttctgta tcccgaccaa agctaaatct
     3181 gacagttcca ttgatttcag attgacttaa tcccatagac tttaatacat gagaaggttc
     3241 atcatcatca gaattacaag ctgaaccttt tgaaacagca gctattttca taccatagat
     3301 aatactttcc gcatagaggc catctatgct gacactcaga atgcctggaa aaacattttc
     3361 tgaatgaccg ttcacgctca atttagaaat atccttgcat gtatctataa agagctctct
     3421 acattgtctt aagtgcctgt gatcactcga cattttttcc tgagcaattt tgtaggctct
     3481 ggccatgcct acaatttggt gggttgccaa ggttccaggc cttagagatc gttcttgacc
     3541 tcccccaatc aaaatgggtt caactctgcc taccgattca tgattgatga ataaagcacc
     3601 gatacccttt ggtccataaa ccttatgggc atttaaagac attaagtcta tcttgtaatc
     3661 tttaacatca atgggtaact tgccaattcc ttgagctgca tcgacatgaa aaaagacatt
     3721 attctgtctg caaagattac caatagactt gatatcgttg atgctcccca cttcgttatt
     3781 aacatgcatc agactcacca tcaatgtttt ctcattgatt gccccctcca attcattaat
     3841 atcaactgaa ccgtttgcat ttggatttag aagggtcagc tcgaaacctt cttttttcac
     3901 ttgcagacat ggttcaatca ctgatttatg ttctgcgaac gatgatataa cttgctgttt
     3961 aaatccattt tttttaaacc tagccaaacc caaaattgct aaattattcg cttcagttgc
     4021 acctgatgtc caaataatct cgcctgcatc agcattgatc aatgaagcaa tatctagcct
     4081 cgcattctct acagcttcat atgcctgttt accaaataaa tggtccaccg acgagggatt
     4141 accaaaggat tgaccactca tcacagcttc catctcagag acaacttccc tagccagggg
     4201 tgtggtcgat gcataatcaa gatagatttg ctttatgatc tctttcatgt gcttgtatca
     4261 ctcttatcct tataagacgc tagaatacct ctcattatat tcatttcatt ttcatccaat
     4321 tgtgctctat tgaatagcgt tctcaatctc ctcatcaatt gtctgggatt tcttgggttc
     4381 aaaaatccag tctccagtaa tacttgatta agatgctcat aaaataattc catttctgaa
     4441 ttggatgcta gatttcttgt ctctatttct gaaggtagtg cttgccaagc aaaatgtaat
     4501 tcataagcga ttatttgaat ggccattgcc aaatttaatg atcgatacaa ttggcttgaa
     4561 ggaatatgaa ttaattgatt gcagcaatca atttgttcat tcatcaatcc tgttttttca
     4621 ggaccaaata atatcgccac tggtttatgt ttggctttat taataatact cgaagcaatg
     4681 tccctgattg ttgatgttgg tgcactgatt cttctagttc tagatgttgt cccaacgact
     4741 aaaccacagt tttcaatcgc atcatttaag ttgtcacaaa cttcggcatc caacaaaacg
     4801 tccaaggctc ccgaagacct tgctctggct tctgggtgtg gatgctcttt tggattaatt
     4861 aaacacaata aatcaaaacc catggtctta atagcacgag cagcagcacc aacatttcca
     4921 ggatgggagg gttcaaataa aacaaattta acttgtgggt tcataaaact attgtgcttc
     4981 caaaataaag tcagatattt agctaaaaag ttttgttatt ctacacatta tctattttct
     5041 atatttatta aacgaatatt aatttaacat gcaagccttc ttaaatactg caatcgaagc
     5101 cgcaagaaaa gcgggagata tcgcacaaat gaactttggt agaatcaatg agagcgatat
     5161 caagaccaaa tcttataatg aatttgtcac cttcgttgat aaacaagccg aagccgctat
     5221 taaagagatt attcagtcac gatatcccga tcacggaatg attgccgaag aaagtggaga
     5281 aaataccaac aataaacagg attttgtttg gattgtagac cccattgacg gtacaacgaa
     5341 ttatatacac tcttttcctg tatacgctgt ttccattgca ttacaaatca aaggagtgtt
     5401 agaagtgggg gtaatttatg atccttctag gcaagaatta ttcaccgcac taaaaggcca
     5461 aggcagtcga cttgatgaaa gacggattag agtcagtaaa caaagcacat tggaaggagc
     5521 attactggga actgggtttc cttatcgtca gaataaagac tggttggata tttatctaga
     5581 aatattctct gaattttctc aaaaagccgc aggtattaga aggccaggag ctgctgctct
     5641 agatctggca tacattgcaa gcgggagatt ggatggattt tgggagtttg gattaaattc
     5701 ttgggatatt gcagctggtg tattgctgat ccaggaatca ggagggttgt gtgaaaattt
     5761 aatccccgat caagacatga ttggatctgg aaatctgttt gcaggaaatc caaaaatttt
     5821 cgatcaaatg aaacaaatca tctccaagta tggtccccaa ctaactcaac tcagctagcg
     5881 taccaatatt cccttgctta gggcatacca tcatcgagtt gttctttatt aacttgaagc
     5941 aaatcactcg tagaaacatc caataaaaat atagaggttg tggcaacgta gatggacgag
     6001 taagttccaa caataacacc aatgatcaaa gccagagaaa atccttctaa agacttgcca
     6061 cctataagat ataaagcaag taacaccaac aatgtggtgc
//
LOCUS       DASCTH010000023         4314 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000034096, whole genome shotgun sequence.
ACCESSION   DASCTH010000023 DASCTH010000000
VERSION     DASCTH010000023.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000034096"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /gene="hflC"
                     /locus_tag="QGH35_00450"
     CDS             <1..716
                     /gene="hflC"
                     /locus_tag="QGH35_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013324.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HJL81036.1"
                     /translation="PFVNNVGKYSKQILTINNPQELFLTLEKKNLFVDFFIKWKINDV
                     QQYYISTGGDELVASQRLLETVKDGIRSEFAKRTVIEVVSEERRKVMSEMLNAAAFNA
                     INLGIELVDVRVKRVELSDEVSESVYNRMRQERARIASELRAEGAEAAAIIRAAADRE
                     VTVIIANAYRDSEKIKGNGDASSTNLYAEAYTQDEEFYSFYRSMEAYKKSIGTGNDIL
                     VLDGEGDFFKYLNQSKTSE"
     gene            732..917
                     /locus_tag="QGH35_00455"
     CDS             732..917
                     /locus_tag="QGH35_00455"
                     /inference="COORDINATES: protein motif:HMM:NF021366.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HJL81037.1"
                     /translation="MGWTEALIGLAFLLILEGLLPFVSPKLFKKAIFEIKELGEMAIR
                     FMGFIFLITGALILYLL"
     gene            932..2224
                     /locus_tag="QGH35_00460"
     CDS             932..2224
                     /locus_tag="QGH35_00460"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HJL81038.1"
                     /translation="MANSIIVIGAQWGDEGKGKIIDLLTIKSDAVVRFQGGHNAGHTL
                     VIEGHETILHLIPSGIFNNADCIIANGVVLHLPTLFDEIDSLKKKGVAIEQNLHISNL
                     CPLILPSHIALDEAKEKNLGSKAIGTTGRGIGPAYEDKIARRAIHLVDLFHQEYFLEK
                     LKDLMEFHNFLLVNLYKTKPINVQSVADEWLSYAEHVQPFVTDTTHLLHSINSSDSNI
                     LFEGAQGTMLDIDHGTYPFVTSSNTVAGAASTGTGLGPSDFNHVLGIIKAYATRVGSG
                     PFPSELSDENGEHLGRVGVEFGATTGRARRCGWLDAVAVKKAIINNSISHLCVTKLDV
                     LDGLEEISIAVQYDYKGEVLSSFPSIPSEELKHCKPIYKNFKGWNQSTAGITKIEDLP
                     REAQHFIDAIESILAKKIVLISTGPGREDNIVIEDLFN"
     gene            complement(2260..2346)
                     /locus_tag="QGH35_00465"
     tRNA            complement(2260..2346)
                     /locus_tag="QGH35_00465"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2310..2312),aa:Leu,seq:cag)
     gene            2395..3135
                     /gene="rlmB"
                     /locus_tag="QGH35_00470"
     CDS             2395..3135
                     /gene="rlmB"
                     /locus_tag="QGH35_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HJL81039.1"
                     /translation="MNEKIYIYGINAIFEVLNNKSDQLIEVLLDNNVSNNRILKLIDI
                     IQKKSINLRRISTQEIEQITGTSKNQGIAAAIKRPAILDSKSALNFIKTIQNPLILIL
                     DGLQDPRNVGACMRTANASGVNLIVFPRSRKIKMTPIISKVSSGASALIQIAEIPNLV
                     RFIKELKKIGLWICGTSEIAETKYWEIDYNLPLAVILGSEGKGMRPLTMKHCDYLVSI
                     PMIGEVKSLNVSVVAGVVLYEALRQRSS"
     gene            3256..3771
                     /gene="rpsF"
                     /locus_tag="QGH35_00475"
     CDS             3256..3771
                     /gene="rpsF"
                     /locus_tag="QGH35_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HJL81040.1"
                     /translation="MRHYEVVLLVHPDQSDQLTDMLKRYQDMVEKDSGKVHRLEDIGR
                     LQLAYNIQDMHKAHYVLINIECSDKTLSEIESSFKFNDSIIRHLIVRMNKAETEPSIL
                     FSENNKQEKQKKEKDKKIIERKNESPLIEKKVVEVKKDKVVETESEVDPGEIETQGSA
                     EQNEDVSDEKG"
     gene            3749..4027
                     /gene="rpsR"
                     /locus_tag="QGH35_00480"
     CDS             3749..4027
                     /gene="rpsR"
                     /locus_tag="QGH35_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HJL81041.1"
                     /translation="MCQMKRDKNKNKFKRRRNLNRNTNRYCKFTAENVDQIDYKDIAT
                     LKKYISETGKIIPSRITGTKNRYQKQLATAIKRARFLALIPYTDRHNR"
     gene            4046..>4314
                     /gene="rplI"
                     /locus_tag="QGH35_00485"
     CDS             4046..>4314
                     /gene="rplI"
                     /locus_tag="QGH35_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HJL81042.1"
                     /translation="MEIILKEAITNLGEIGDVVTVKPGYARNYLVPYGKAAYATPENL
                     KILEYDKAELERKQQEEIEKIKLRSVEYENLTIEIQANVTEEGNL"
BASE COUNT         1568 a          670 c          855 g         1221 t
ORIGIN      
        1 tacccttcgt aaataacgtt ggaaagtatt ccaaacagat tctaaccatc aataatcctc
       61 aagagttgtt tttgacctta gaaaagaaaa atttatttgt cgactttttt attaaatgga
      121 aaatcaacga tgtccagcaa tactacattt caaccggtgg tgacgagcta gtggcgtcac
      181 aacgtctatt ggaaactgtc aaagatggaa tcagatctga atttgcaaaa cgaactgtca
      241 tagaagtggt gtcagaagag agaagaaaag ttatgtctga aatgcttaat gctgcagctt
      301 ttaatgcaat aaatcttggg atagaactcg tcgatgtgag agttaaaaga gttgaactct
      361 cagatgaagt tagcgaatca gtatacaacc gcatgagaca agagcgagca aggattgcat
      421 cagaattgag agccgagggt gcagaagctg ctgcgataat tagagcagca gcagatagag
      481 aagtgaccgt tatcattgcc aatgcttaca gggattctga gaagataaaa ggtaatggtg
      541 atgcttcttc tactaacttg tatgcagaag catacaccca ggatgaggaa ttttattctt
      601 tttacaggag catggaagcc tacaagaaat caattggaac cggcaatgat atcttggtac
      661 tggatggcga aggagatttt tttaaatacc tgaaccaatc aaaaacatct gaataatcag
      721 taaaaaacca aatgggatgg acagaggcgt taattggatt ggcttttttg ctaatactcg
      781 aaggccttct cccttttgtt tctcctaaac tgtttaaaaa agccatattt gaaattaaag
      841 aacttggaga aatggctatt cggtttatgg ggtttatttt tttaataaca ggagcattga
      901 ttttatatct tttataaatc aaggatagat cttggcaaac tcgattattg taataggagc
      961 tcaatgggga gatgaaggca aaggtaaaat catagacctg ctcaccatta aaagcgatgc
     1021 tgtagttaga tttcagggtg gacataacgc tggtcatacc ttagttattg aaggccatga
     1081 aaccatactt catttgatac cttctggaat ttttaataac gctgattgta ttattgccaa
     1141 cggggttgta ttacatctcc caactttatt tgatgaaatt gactcactta agaaaaaggg
     1201 agtggcaata gagcaaaatc tacacatcag taatttatgc cctttaatac ttcccagcca
     1261 tattgcttta gatgaggcta aagaaaaaaa tcttggatct aaagctattg gaactacagg
     1321 tcgaggaatc ggaccagctt atgaagacaa aatagccaga agagctatcc atttagtaga
     1381 tctttttcat caggagtatt ttttagaaaa gcttaaagat ctgatggaat tccataactt
     1441 tttattggtt aatttatata agacaaaacc aatcaatgtt caatcagtgg cagacgaatg
     1501 gttatcgtat gcagagcatg tacaaccatt tgttacagat acgactcact tattgcattc
     1561 tattaatagt tctgattcga acatcctatt tgaaggagct caaggaacaa tgttggatat
     1621 tgatcatgga acttatcctt ttgttacttc ttcaaatact gttgcaggag cagcttcaac
     1681 aggtactggg cttggaccaa gtgactttaa tcatgtcctt ggcattataa aagcctatgc
     1741 aaccagagtt ggttcaggac cattcccctc ggaactttct gatgaaaatg gtgaacattt
     1801 aggtagggta ggcgttgaat tcggagcaac aacaggaagg gcaaggagat gtgggtggtt
     1861 ggatgctgtt gccgtgaaaa aggctatcat aaacaacagt atctcgcatt tatgtgtcac
     1921 aaaattagat gttcttgatg gtttagaaga aatatcaatt gcggttcagt atgattataa
     1981 aggcgaagtc ctttcatctt ttccatcaat accaagcgag gagttaaagc actgcaaacc
     2041 aatttataag aattttaaag gttggaatca atccactgca ggcattacaa aaattgaaga
     2101 cttgcctaga gaagcgcaac attttattga cgccattgaa tctattttag ctaaaaaaat
     2161 tgttttaatt tctactggtc ctggtagaga agataatatt gttattgaag atttgtttaa
     2221 ctaaatcaat ttggattaat tttttgacat aatagtcatt ggtgccgagg agaggacttg
     2281 aacctccacg gggtttcccc cactggcttc tgaagccagc gcgtctacca attccgccac
     2341 ctcggctaaa aggatataat caatagtaaa ggaaaaatta aattaccaaa ataaatgaat
     2401 gaaaaaatat acatttatgg gattaatgcc atcttcgagg tgcttaacaa taaatcagat
     2461 caattgattg aagttttgtt agataacaat gtttctaata ataggatttt aaaacttatt
     2521 gatattattc aaaaaaaatc aattaatctt agaagaattt caacacaaga gattgaacag
     2581 ataaccggaa catcaaaaaa tcaaggaatt gcagcagcaa taaagcgacc agcaatttta
     2641 gattcaaagt cggcattaaa ttttataaaa actattcaaa atccattaat tctgattctt
     2701 gatggattgc aagatcctag aaacgttgga gcgtgtatga gaaccgctaa tgcatcaggt
     2761 gtcaatttaa ttgtttttcc aagaagcaga aaaataaaaa tgacacctat tatcagcaaa
     2821 gtgtcttcag gagcctcagc tttaatacaa attgcagaaa ttccaaacct agttagattt
     2881 ataaaagaac taaaaaaaat aggactgtgg atttgtggaa cctctgaaat tgctgaaaca
     2941 aaatattggg agatagatta taacttgcca ctcgccgtta ttctcggatc agaaggcaag
     3001 ggcatgagac ctttgacgat gaagcattgt gactatttag tctctatacc aatgattggg
     3061 gaagttaaaa gtttgaatgt ttcagttgtt gctggagttg ttttatacga agcactgcgt
     3121 caaagatcat cataatggtt tgaatatctt tattaattaa agtagcatcc tgcatttatt
     3181 aagaatagaa tagctaccaa tcaaccttgc tgcacttgtt atatgataag gcagctctaa
     3241 gccaaaagga gatttatgag acattatgaa gtagttcttt tagtgcatcc tgatcagagc
     3301 gatcaattga ccgatatgtt aaaacgctat caggacatgg tggaaaaaga cagtggaaaa
     3361 gtccatcgat tagaggatat aggtcgtctt caattggcct acaacatcca agatatgcat
     3421 aaagcacatt atgtgcttat caacattgaa tgtagtgata agaccttatc agagattgaa
     3481 tcttcattta aatttaacga ttcaatcatc agacatttga ttgttcgaat gaacaaagct
     3541 gaaacagagc cttcgatatt attttccgaa aataataaac aagaaaaaca aaaaaaagaa
     3601 aaagataaaa aaatcatcga gagaaagaat gaaagcccat taattgaaaa gaaggtcgtt
     3661 gaagtaaaaa aagataaagt ggtggaaact gaatctgaag ttgatccagg tgaaatagag
     3721 acacaagggt cagcagaaca aaatgaggat gtgtcagatg aaaagggata aaaataaaaa
     3781 taaatttaaa agaagaagaa atctcaatcg gaacacaaat agatactgta agtttacggc
     3841 agaaaacgtt gatcaaattg attataagga cattgcgacc ttaaagaaat acattagtga
     3901 aacaggaaaa ataattccaa gtcgaattac tgggacgaaa aatcgttacc agaaacaatt
     3961 agctaccgca attaaacgtg ctagattctt ggcactgata ccttatacag atcggcacaa
     4021 tagataaaaa aaatatgaga cgataatgga aattattcta aaggaagcaa taacaaattt
     4081 gggtgaaata ggtgatgtag tcactgttaa acctggatat gcaagaaatt atcttgttcc
     4141 ttatgggaaa gctgcttacg cgacgccaga aaatttaaaa atattggaat atgacaaagc
     4201 agaactagag cgcaaacaac aagaagaaat agaaaaaatt aaattacgct ctgttgaata
     4261 tgaaaatctg actatagaaa tacaagctaa tgttacagaa gaaggcaact tata
//
LOCUS       DASCTH010000024         4613 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000034796, whole genome shotgun sequence.
ACCESSION   DASCTH010000024 DASCTH010000000
VERSION     DASCTH010000024.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000034796"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="QGH35_00490"
     CDS             complement(<1..185)
                     /locus_tag="QGH35_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81043.1"
                     /translation="MEFKTLATQESEKKLFLNRIIFSIIVIVFLIIALLLRLIQLQIL
                     DSDQYKLRSMNNTIRTYS"
     gene            complement(194..676)
                     /gene="mreD"
                     /locus_tag="QGH35_00495"
     CDS             complement(194..676)
                     /gene="mreD"
                     /locus_tag="QGH35_00495"
                     /inference="COORDINATES: protein motif:HMM:NF016019.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HJL81044.1"
                     /translation="MSKRIKNRFFIWSILMISSSLLVIPLPDNFAFIRVPWLPLSVIF
                     FSIMTPSFFGILSAWFIGFVADLLQGDILGQNAVALSVLAYISYRFHLQIRVFPIWQM
                     MIVILLLLSLNELILLWVQGISGQIFFNYTRWIAIVIGALIWPIFMTLLLNIKSKVDW
                     "
     gene            complement(669..1526)
                     /gene="mreC"
                     /locus_tag="QGH35_00500"
     CDS             complement(669..1526)
                     /gene="mreC"
                     /locus_tag="QGH35_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HJL81045.1"
                     /translation="MPLSTETTVAQRHRYSAYRLVFTLIFSFGIMYLDFNGKYLNTLR
                     SYLSAAVYPVHVLVNTPKNLFSAISGNINKQEKLDLINQDIKKENIILKSKIQQIYKL
                     ESENKRLRALLDSKPEHQNELIFAEIVSINNDVNKHRIMINKGSRENVYSGDVVSDSK
                     GIIGQIIRDHIFGSEVLLITDPEHAIPIEVARNGLRSIAIGVGNYDHIVLNYLPISAD
                     INKGDVLITSGLGGKYPEGYPVATVESIANLTGEPFLEIEAKPFAELQNINEVWVIKQ
                     SITQPFNIE"
     gene            complement(1565..2608)
                     /locus_tag="QGH35_00505"
     CDS             complement(1565..2608)
                     /locus_tag="QGH35_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HJL81046.1"
                     /translation="MFKTLTGYFSNDLSIDLGTANTLVYSRGKGVVLNEPSVVALRTE
                     HGKPGKTVVAVGTDAKAMLGRTPGTIEAIRPLKDGVIADFIVTEAMLQAFIKKAHRSK
                     YFRPSPKILVAVPYGSTQVERRAIRESAEGAGARKVFLIEEPMAAAIGAGLPVHEARG
                     SMVLDIGGGTSEIAVISLNGIVYGLSIKIGGDTFDEAIINYVRRNYGMLIGEATAERI
                     KHEIGSAYPGETLEDITVKGRNLSEGVPRAFSLNSNEILDSLQEPLQAIANALKSALE
                     QTPPELGSDISDRGIVLTGGGALLKNTDKFLMEETGLPVLIADDPLSCVAKGGGKVIE
                     LLEEHGPDHFDLT"
     gene            2721..3008
                     /gene="gatC"
                     /locus_tag="QGH35_00510"
     CDS             2721..3008
                     /gene="gatC"
                     /locus_tag="QGH35_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HJL81047.1"
                     /translation="MSIKKTDLEYIASLSKLQFEGQELKNYTRQISDILEMIQQLQKV
                     DTKKIKPMAHPMNMNQRIREDLVTEVNKRDEYQKNAVDSEDGFYKVPKVLD"
     gene            3064..4506
                     /gene="gatA"
                     /locus_tag="QGH35_00515"
     CDS             3064..4506
                     /gene="gatA"
                     /locus_tag="QGH35_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HJL81048.1"
                     /translation="MLGEEKISSQEILKTYIQRIAEHDTLINSFITLDADAALEKARQ
                     ADQSRQSGDVLPLTGIPFASKDIFCTKGVKTTCGSKMLSNFVPPYDATVIANVNEQGA
                     ILLGKTNMDEFAMGSSNETSYFGPVNNPWDLERTPGGSSGGSAAAVALGLIPIAIGTD
                     TGGSIRQPAAFTGITGLKPTYGRCSRYGMIAFASSLDQAGILALSAEDTAIFLEMMAG
                     FDPLDSTSVEMDVPSYVEQLSDPINGKVIGIPKEFFDEGLDAELENKILDAIKIYESL
                     GVKFKEISLPHLELSVPTYYVIAPAECSSNLARYDGIRFGYRHQDNGRNNLDDHYLTS
                     RQSGFGAEVKRRIITGTYVLSAGYYDAYYLQAQKVRQLISQDFKRAFLDVDAIIGPTT
                     QTPAFKIGEKIDDPVTMYMNDIYTISSNLVGLPAISHPCGFIDGLPIGVQIITPHFAE
                     CDLLNLTHLYQQKTDWHNQQPDINNTNDDN"
     gene            4522..>4613
                     /locus_tag="QGH35_00520"
     CDS             4522..>4613
                     /locus_tag="QGH35_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006459912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81049.1"
                     /translation="MGWEAVIGLEIHAQLACKSKIFSSSSTEYGA"
BASE COUNT         1461 a          881 c          882 g         1389 t
ORIGIN      
        1 gagtaagtcc gaatagtgtt attcatggat cggagcttat attgatcact gtcaagtatc
       61 tgtaattgga tcaacctgag gagcagagca ataatgagaa atactattac aattattgaa
      121 aaaataattc tattcaaaaa taattttttc tcagattctt gagttgctag agttttaaat
      181 tccatggatt taattaccag tctacttttg attttatatt cagtagaagt gtcataaata
      241 taggccagat aagtgcacca ataacgattg ctatccagcg ggtataattg aaaaatattt
      301 gaccactaat tccttgtacc cacaataata ttagctcatt caatgacagc aacaataaaa
      361 tcacaatcat catttgccat attggaaaaa ctctaatttg taggtgaaat ctataactaa
      421 tataagcaag aacggacaat gcaacagcgt tctgtccaag aatatcaccc tgcaagagat
      481 cagcaacaaa acctataaac caggcgctga gtattccaaa gaagcttgga gtcattatcg
      541 agaagaagat gaccgaaagg ggcaaccaag gaactctaat aaatgcaaaa ttgtctggca
      601 atgggataac cagcaaggag ctcgagatca tcaatattga ccaaatgaaa aatcgatttt
      661 ttattctttt actcaatgtt gaatggctgt gttattgatt gttttatgac ccaaacttca
      721 ttgatgtttt gtaattcagc aaaaggtttt gcttcaatct caagaaatgg ctcccctgtt
      781 aagtttgcta tcgattccac agttgcaaca ggatatccct ctggatattt tccacccaac
      841 cctgaagtaa tcaagacgtc tcctttgtta atatcagcac tgataggtaa ataattcagc
      901 actatgtgat cgtaattgcc tacacctatt gcaatagaac gtaacccatt tctagcaacc
      961 tcaatgggta ttgcatgctc tggatcagtg attagaagaa cctctgatcc aaaaatatga
     1021 tcacgtatga tctggccaat aatgccttta gaatctgaga caacatcacc gctataaaca
     1081 ttttctcggc tgcctttatt gatcataata cgatgtttat ttacatcatt attaatagat
     1141 actatttcgg caaatattaa ttcattttga tgttctggtt ttgaatcaag gagcgcacga
     1201 agtcttttat tttcagattc tagcttataa atttgttgaa tttttgattt cagtattatg
     1261 ttttcttttt ttatgtcttg atttataagg tctaattttt cttgcttatt gatattccct
     1321 gaaatggcag aaaataaatt ttttggagta ttgacaagga catgaactgg atatactgct
     1381 gcagaaaggt agcttctcag tgtatttaag tatttgccat tgaaatccaa atacattatc
     1441 ccaaacgaga aaataagtgt aaaaaccagt cgataggctg aatacctatg tctttgtgca
     1501 actgttgttt cagtggataa tggcatctta aattatcaac cgctcgagat aatcttaaga
     1561 gaaatcaagt caaatcaaaa tggtcaggtc catgttcctc aagaagttca atcaccttac
     1621 caccaccctt cgcgacgcat gaaagaggat cgtccgcaat cagaacaggc aaccctgttt
     1681 cttccattaa gaatttgtca gtatttttga ggagcgctcc accaccggta agaactatac
     1741 ccctatcgga aatatcagat cccaattcag gtggcgtttg ttccagtgct gatttgagag
     1801 cattggcgat agcctgaagc ggttcttgta acgaatcaag aatttcattg ctgtttaatg
     1861 aaaaggctct cggaacgccc tctgagagat ttcgcccttt aactgtaatg tcttcaagag
     1921 tttctcctgg gtaggcagag ccaatttcat gtttgattct ttcagccgtt gcttcaccaa
     1981 tcagcatccc ataatttctt ctgacatagt tgataattgc ttcatcaaaa gtatcacccc
     2041 caatctttat agaaagtcca taaactatac cgttgagcga tataacagca atttccgatg
     2101 tgccgcctcc aatatcgaga accattgatc ctcttgcttc atgcacaggt aaaccggcac
     2161 ctatcgcggc tgccattggt tcttctatta aaaaaacttt tcttgcgcca gctccctctg
     2221 ctgattccct aatcgcacgt ctttccacct gtgtcgaccc ataaggaaca gcaaccaaga
     2281 tttttggact gggtctaaaa tatttactac gatgggcttt ctttataaag gcttgtaaca
     2341 tggcttctgt gactataaag tctgcaatga caccatcttt cagtggtcgt attgcttcaa
     2401 ttgttcccgg tgttctgcca agcattgctt ttgcatcagt gccaactgca actactgttt
     2461 ttcctggttt tccatgttct gttcttaatg ctacaactga tggttcatta agaacgacgc
     2521 cttttcctct tgaataaaca agggtattcg ctgtgcccag atcaatggat agatcattag
     2581 agaaataacc tgttaatgtt ttaaacattt aatctcaatg gatagaataa taagtctgag
     2641 atcactactc taacaacgtg taggattttg agcaactgga agttatcata acaagtaatc
     2701 ttacattgct tagacagatc atgagtatca aaaaaacaga cctagaatat attgcatcct
     2761 tatctaaact gcaatttgag ggtcaagaat taaaaaatta cacccgtcaa atctctgata
     2821 ttttggagat gatccagcaa ttgcaaaaag ttgacacaaa gaagatcaag ccaatggcac
     2881 acccaatgaa tatgaatcag agaatcaggg aggatttggt tactgaagtt aacaaaagag
     2941 acgaatacca aaagaacgcg gtagattctg aggatggttt ttataaggtt cccaaggttc
     3001 tagattaaat atgtttgagg ggagaaggta ttgcagcgcc acacactaaa aaatttactc
     3061 aacatgttgg gtgaggagaa aatctcaagc caggagattc tcaagacgta tatccaaaga
     3121 attgcagagc atgacacatt gatcaattct tttattactt tggatgcaga tgccgcattg
     3181 gagaaagcaa gacaagccga tcaatcaaga cagtcaggag atgttttacc tttgaccggt
     3241 attccttttg cgagtaaaga tattttctgt actaaaggag tcaaaacaac ttgtggctca
     3301 aaaatgcttt ccaattttgt tcctccctat gatgctactg tgattgccaa tgtaaatgaa
     3361 cagggtgcga ttcttttggg taaaaccaat atggatgaat ttgcaatggg ttcatccaat
     3421 gaaaccagct attttggacc ggtcaacaat ccttgggatt tggaaaggac accgggggga
     3481 tcttctggag gatcagcagc agcagtggca ttggggctaa tacctatagc aattggcacc
     3541 gatacgggag gatcaataag acaacctgca gcctttacag gtattacagg tttaaaacca
     3601 acctatggca ggtgctcgag atatggcatg attgcttttg catcaagctt agatcaagca
     3661 ggtattctgg ccctttctgc tgaagataca gcaattttct tagaaatgat ggctggcttt
     3721 gatcctttag actcaaccag tgtagagatg gatgttccca gttatgttga gcaactatct
     3781 gatcctataa atggcaaggt cattgggatt cccaaagaat tttttgatga gggattggat
     3841 gctgaactgg aaaataaaat actggatgcc atcaagattt atgagagtct gggggtcaaa
     3901 tttaaggaaa tttcactgcc ccatctcgaa ctttctgtac caacatatta tgtgattgca
     3961 cctgctgaat gctcatctaa tttagccaga tatgatggca taagatttgg ctatagacac
     4021 caagataatg gcagaaataa tttagatgat cattacctta ccagtcgtca aagtggattt
     4081 ggggcagagg tgaagaggag aataatcact ggaacatatg ttctatcagc tggttattac
     4141 gatgcttact atttacaagc acagaaagtt aggcaattaa ttagccaaga ctttaagagg
     4201 gctttcttag acgttgatgc aatcattgga cccacaacac aaacaccggc atttaaaatc
     4261 ggtgagaaaa ttgatgatcc cgtcacaatg tatatgaatg acatttatac catatcatct
     4321 aacttagttg gattgcctgc aatttcacat ccatgtggat ttatcgatgg acttccaatt
     4381 ggtgtacaaa ttatcacccc ccattttgca gaatgtgacc ttttaaatct aacgcatcta
     4441 tatcaacaaa aaacagattg gcacaatcaa caaccggata taaacaatac taatgatgat
     4501 aactaaacag gaatgaaagc aatgggatgg gaagctgtta ttggcctcga gatacatgcg
     4561 caattggcat gtaagagtaa gatattttca agttcttcaa cagaatatgg cgc
//
LOCUS       DASCTH010000025         9960 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000035565, whole genome shotgun sequence.
ACCESSION   DASCTH010000025 DASCTH010000000
VERSION     DASCTH010000025.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000035565"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="QGH35_00525"
     CDS             <1..122
                     /locus_tag="QGH35_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81050.1"
                     /translation="EDVGNAAAFLCSDLASGITGEVLYVDGGYHNLGMTLNID"
     gene            complement(146..2050)
                     /locus_tag="QGH35_00530"
     CDS             complement(146..2050)
                     /locus_tag="QGH35_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025010.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HJL81051.1"
                     /translation="MLEIIREKFTGWIAILIISAIAIALVISFGNMDQTPLEQNIVIT
                     VNDKEISLVEYREEFSNQLMQIQEILGNQIPDSLEYTIKEAAVENLIMKTLLKDYVHA
                     QGYRVSPEFVTELIMTTPQFQLGESFNRENYEAMLLSQGVSLSKYENDLRIQLQINQL
                     RNGLTGSSFITPFEFRKFVTLQLEEREGEYLMIPAAHFAEQVKLDSDEVLSYYEENEE
                     SFVTEEEIDVEYLSISIEEIAQSIEFSPLDVQQYYDENIERFRSNEERKSSHILISFE
                     DDSEVDAFEEIKNIDQRIKNGESFEALATEFSDDPGSASNGGDLGWAEPGSFVEEFEQ
                     VLFSLGNGEISDPIKTQFGYHLIRLDDIKEGRQQFFSEIEDELTDEYANVLAKDRLYD
                     VAEQLDDLSLQAYNELNSVANVLSLQLKQVKSVSRNGRSFLNEQAEFIDILFSPSSLE
                     QSENTPVFEFDDFIIVARVTNHRLPEMKTFEQVEGEITEFLISEQSVDLATTFAQEKT
                     KELASDSVLNEVLELYDLELQNFTALKRNDNTLPKVFLDAIFDIPGKDIGRNRYHFVE
                     VNEMMYLFSVLQMNPGRLDAFSAEERDGGKIALAEQFGSNEVSSFTKALREKAKVEID
                     PDLFNSAYDL"
     gene            complement(2261..3166)
                     /locus_tag="QGH35_00535"
     CDS             complement(2261..3166)
                     /locus_tag="QGH35_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015558.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HJL81052.1"
                     /translation="MTYLLLAIFITLSLVIDQAAISLLLGIFFSVIKEKYSLTSTKIN
                     GANFLKAGIILLGVTISVDSVSEISANYLPLVSFYVLMVFFVGFLLGRVLKVSKKHLI
                     LLVAGTAICGGTAIASLAPVIQSKTEELVSSISLIFLLNLLAIIFFPLIGQYFGMSEK
                     EFGIFAALTIHDTASVLGAASIYGDDSVMYASVIKLGRTLWIIPVIICFSLYFKNKEA
                     KYNFPYFIIFFLIFVLLGTFIDLNQDIQIIIQKISNTLFLLGLFFIGSQFKLNTLKEL
                     SIQPIIFAVTLWLIIIITTILVFYV"
     gene            complement(3240..3317)
                     /locus_tag="QGH35_00540"
     tRNA            complement(3240..3317)
                     /locus_tag="QGH35_00540"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3280..3282),aa:Asp,seq:gtc)
     gene            complement(3421..3496)
                     /locus_tag="QGH35_00545"
     tRNA            complement(3421..3496)
                     /locus_tag="QGH35_00545"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3461..3463),aa:Val,seq:tac)
     gene            complement(3674..3946)
                     /locus_tag="QGH35_00550"
     CDS             complement(3674..3946)
                     /locus_tag="QGH35_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014994723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HJL81053.1"
                     /translation="MNKGDLIDAVAASTGSSKADAGRAVDAVTSAIAGELGGGGSVSL
                     VGFGTFSVSHRAARQGRNPATGESIWINASNVPKFKAGKALKAACN"
     gene            complement(4107..6542)
                     /gene="lon"
                     /locus_tag="QGH35_00555"
     CDS             complement(4107..6542)
                     /gene="lon"
                     /locus_tag="QGH35_00555"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HJL81054.1"
                     /translation="MPNKKEEKIFLDIVLDKPLLPLRDVVVYPHMVIPLFVGREKSKM
                     ALEEAMIHEKKILLSSQKRSDIDDPDFKDINTVGTLANILQLVTLPDGTVKLLVEGES
                     RVDLLEIRSNDYFTADYCILSEKYHEGDKEIKALRRTLLSQVESYVRITKKVPAEVVN
                     TLANIKEPGRLVDTIVAHMSLKLEEKQKVLELINVKARLEHVVALVDVEIDMVEMEQK
                     VRGRVKDQMEKSQKEYYLNEQMKAIQKELGDMDNASNELDDLENKIKKSGMSEEAAKK
                     ANSELKKLQMMSPMSAEATVVRNYLDALIDAPWKKKTKAKIKLDKAEQILEEDHYGLK
                     SVKERITEYLAVQQRTKKLKGPILCLVGPPGVGKTSLGSSIARSTGRKFIRMSLGGVR
                     DEAEIRGHRRTYIGAMPGKIIQNLSKAGVRNPLFLLDEVDKMTMDFRGDPSSALLEVL
                     DPEQNSTFNDHYLEVDFDLSDVMFVCTANTALNIPPPLLDRMEVIRIEGYTEDEKLNI
                     ADKYLVDKQMQMNGLKKGEIKFTKSAILSIIRHYTREAGVRNLERDIAKIARKVVKSL
                     TIKKEKKTTHIRPSGLDKYLGVRKFKYGIAEKENQIGQVTGLAWTQVGGELLTIESTV
                     IPGKGKLINTGQLGDIMKESIQAATTIVRSRAKSLGIDEEFYQKVDLHVHVPEGATPK
                     DGPSAGVGMCTALVSSLTEIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAHRGGIST
                     VIIPDENSKNLIEIPKNIKEKLTIIPVKWIDEVLEIALEKMPNISSEEKEKMEVERTS
                     VTKKSSLESNLPH"
     gene            complement(6634..7956)
                     /gene="clpX"
                     /locus_tag="QGH35_00560"
     CDS             complement(6634..7956)
                     /gene="clpX"
                     /locus_tag="QGH35_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HJL81055.1"
                     /translation="MSDKTKETNKDSKILYCSFCGKGQNEVRKLIAGPSVFICEECVD
                     LCNNIIDEELNEASSREGLNLYKPKEINAMLDDYVIDQNDAKKVLSVAVYNHYKRLNN
                     KPNKRNEEDVEIGKSNILLVGPTGCGKTLLAQTLARILNVPFAIADATTLTEAGYVGE
                     DVENIIQKLLQSCDYDIEQAQTGIIYIDEIDKISRKSDNPSITRDVSGEGVQQALLKL
                     IEGTIASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLDKIIQSRTNKSGIGFIAD
                     LREASENKDIGQIFKNIEPEDLIRYGLIPEFVGRMPVVASLSELNEHALIRILTEPKN
                     ALVKQYQKLFAMEKCELEFLSEALETIAKLAMRRKTGARGLRTIMEDTLLDTMYEIPS
                     YNKTKKIVVNKAFVEKQSKPFLVFSSGDQKDSQAELDAEDDSLLVNAD"
     gene            complement(7961..8593)
                     /gene="clpP"
                     /locus_tag="QGH35_00565"
     CDS             complement(7961..8593)
                     /gene="clpP"
                     /locus_tag="QGH35_00565"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018934720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HJL81056.1"
                     /translation="MNQLNEELNLIPMVVEQTPRGERAYDIYSRLLKDNVIFAVGPIE
                     DTMANLIVAQMLFLESENPKKDISLYINSPGGSITSGLSIYDTMQFVKPDISTICVGQ
                     AASMGAILLAGGAKGKRYSLPNSRMMIHQPVSGFQGQASDIDIHAKEILSMRERVNEI
                     LSSHTGKSIKEVTSDTDRDNFMNASNALDYGLIDTILSNRNELIKSQVNG"
     gene            complement(8595..9896)
                     /gene="tig"
                     /locus_tag="QGH35_00570"
     CDS             complement(8595..9896)
                     /gene="tig"
                     /locus_tag="QGH35_00570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HJL81057.1"
                     /translation="MSNLKIKVNSKKGLVRDIHISVPSNVIEEKRNTRFSQLSKTAKL
                     PGFRPGKVPEKVIRRQYADQVLQEVLSDVLETTYMESIQEKNLQPAGPPKVALDEYVK
                     EKDFSYTATIEVYPEFDLKGLDKIKVEKPHADITQKDINAMMDNLQNQKGTWEKVDRI
                     SKGGDQLLIDFEGKIDGVVFDGGTAKDFAMQLGNGQMLPEFDESLIGVKSEETKTIEL
                     TFPDNYHKKELVGKQALFTVDVKEVRELKPAELDQKFVQSFGIKSGDQKELMDEVKSS
                     MSKELEARINNEIRQNLMKFLKDKNAIEIPEVMIHQEAHAMQKDWMNRSGIEDHEQAP
                     PLDDFNEIAKERVHLGLLVSKLVQSKEIKLDPDKVKTKLDELSKTYPNPEEIRKMYEQ
                     SSQLMDQLKSLVMEDQVIDWLIERTEFQKKEMEFKELVNRS"
BASE COUNT         2863 a         2106 c         1593 g         3398 t
ORIGIN      
        1 ttgaggatgt tggcaatgct gcagctttct tgtgttctga tctcgcctct ggaattaccg
       61 gagaggtgct ctatgtcgat ggtgggtatc acaatctggg aatgacccta aatattgact
      121 gattcttatt tcttaataat aaatcttata aatcataagc actgttaaaa agatctggat
      181 caatctcaac tttggccttt tctctcaacg ccttagtaaa agaagacact tcattggaac
      241 caaattgctc agcaagggca atttttcctc cgtctcgttc ttcagcagaa aaagcatcta
      301 gtctacctgg gttcatttgt agcaccgaga aaagatacat catttcatta acttcaacga
      361 agtgataacg gttcctgcca atatcttttc ctggtatgtc aaatattgca tccaaaaata
      421 ctttgggcaa agtgttatcg tttctcttta atgcagtgaa attctgcaat tctaaatcgt
      481 acagttctaa tacctcattc aaaacactat cagaggcaag ttctttcgtc ttctcttgag
      541 caaatgttgt agctagatca actgattgtt ctgatatcag aaattcagtt atttccccct
      601 caacctgttc aaaggtcttc atctcaggca atcgatggtt tgtcactctt gcaacaataa
      661 taaaatcatc aaattcgaat acaggcgtgt tttctgattg ttcaagcgag gaagggctga
      721 atagtatatc gataaattct gcttgttcat tgaggaaaga tctaccattt ctactgaccg
      781 atttaacctg ttttagctgt aatgacaata cattagcaac agaattcaat tcattgtatg
      841 cctgcaatga cagatcatcc aattgttcag caacatcata tagacgatct tttgccaata
      901 catttgcata ttcatcggtt aattcatctt caatctcaga gaagaattgt tgcctgcctt
      961 ccttgatgtc atctaaccga atgaggtggt agccaaattg agttttgatt ggatcagaaa
     1021 tttcaccgtt accgagtgaa aataagactt gctcaaattc ctcgacaaaa gaacccggtt
     1081 cggcccaacc taaatcacct ccatttgagg cggatccagg atcatctgaa aattcagttg
     1141 ctagagcttc aaacgattct ccatttttaa ttctttgatc aatgttcttt atctcctcaa
     1201 atgcatccac ttcagagtca tcctcgaatg aaatcaagat atggctggat tttcgctcct
     1261 cattagatct aaatcgttcg atattttcat cataatattg ctgaacatcc aacggtgaaa
     1321 actcaattga ttgtgctatt tcttctatgc taatagatag atattcaaca tctatctctt
     1381 cttctgttac aaaagattct tcgttctcct cgtaatatga cagaacctca tcagaatcaa
     1441 gcttaacctg ttcagcaaaa tgtgctgcag gtatcataag gtattcaccc tctcgttcct
     1501 ctaattgtaa tgttacaaat tttctgaatt caaaaggagt tataaatgat gatccagtta
     1561 acccatttct cagttgattg atttgtaatt gaatcctaag atcattttca tattttgaca
     1621 agctcactcc ttgactcagt agcatcgctt cgtaattctc acgattaaaa ctttccccca
     1681 gttgaaactg aggagttgtc ataattagtt cagtaacaaa ctcaggactg acccgatatc
     1741 cttgtgcgtg tacgtaatcc tttagcaatg tcttcattat tagattttcg acagcggctt
     1801 ccttaatggt gtattccagc gaatctggga tttgatttcc tagaatttct tggatttgca
     1861 ttaattgatt actaaattct tcccgatatt caactagact aatttccttg tcattgaccg
     1921 ttatgacaat attctgctca agaggtgttt gatccatatt gccaaaacta atcaccagtg
     1981 caattgcaat tgcactaata attaaaatag caatccatcc tgtgaacttt tctctaataa
     2041 tttctaacat agttactaca caagtttata atcaagtggc ggagtggacc gaattatttg
     2101 tcctatcctt ttattcctta actttcctct ctaagttttg tatttattaa ctttccctct
     2161 acaatattcg ttaaatactt gctacatttc tagctacatt tacatgaaga taacttcagc
     2221 aacaagcccg ttatttccta caccaacaat gataattttt tcagacataa aacactagga
     2281 ttgttgtaat tattataatt aaccataagg taacagcaaa aataatgggc tgaatagaaa
     2341 gctccttaag tgtattgagt ttaaactgag aaccaataaa aaatagtcca agtaaaaaca
     2401 aagtgttgct aatcttttgt atgataattt ggatatcctg gtttaaatca ataaatgttc
     2461 ctaataagac aaatattaga aaaaaaataa taaagtaagg aaaattatat tttgcctcct
     2521 tatttttaaa ataaaggctg aaacatataa tcactggaat gatccataag gtcctaccta
     2581 atttaataac actcgcatac attactgaat catctccata aatgcttgcg gcacctaaaa
     2641 cagaggctgt gtcatgaatt gttaatgcag caaatattcc aaactctttt tctgacatac
     2701 caaaatattg accaatgaga ggaaaaaaaa taatagccaa caaattaagt aagaaaataa
     2761 gactgattga agataccagc tcttccgtct tagattgaat aaccggagct agtgatgcta
     2821 tagcggtccc accacaaata gctgtcccag caaccagaag gatcaggtgt ttcttagaaa
     2881 cttttaagac tcttccaaga agaaatccaa caaagaaaac catcaagaca tagaatgaga
     2941 caagaggtaa atagtttgca ctaatttccg aaacagaatc tacgctgatc gttacaccta
     3001 ataaaatgat tccagctttc aaaaaattag caccattgat cttagttgaa gttaaggagt
     3061 atttttcttt tattacagaa aagaaaatac ctaatagtag gctgatagct gcctgatcta
     3121 taacaagaga caacgtgata aatatagcca acaataaata agtcattata aaagtttctt
     3181 ttgctactat tttgttacag aaaaatgaaa gccctatatt acaagggctt cacaggttct
     3241 ggcggagtgg acgggactcg aacccgcgac ctcctgcgtg acaggcaggc attctaacca
     3301 aactgaacta ccactcctct acttaattat tatcaagatt atagaaccat ccgcaattat
     3361 actttgaatt taattacaaa taagtgaaaa aatctatgga ctgtttgtta aaaaatagag
     3421 tggtgggtgc tgacgggatc gaaccgccga cattcgcctt gtaagggcga cgctctacca
     3481 gctgagctaa gcacccgtgt gcaacctcta ataatttatc aaatccttat ttataacaag
     3541 ttcaactact cattcaactc gcgatgatac atcatctgtt agtctgtttc actatcagag
     3601 aggacaaacg aaaagaggac tgtgttcacc tgagtgaacc caatcctctt atttatagcc
     3661 ttgttactat ctgttagtta caagcggctt ttaaggcttt gccagcttta aactttggca
     3721 cattacttgc attaatccaa atgctctcac ctgtagctgg atttcgaccc tgtcttgcag
     3781 ctcgatggct aactgaaaat gttccaaaac caacgagtga aacagaacca ccgccgccta
     3841 attcgccggc gattgcagaa gtaacagcat caactgctct ccctgcatct gcttttgaag
     3901 aacctgtact tgctgctact gcatcaataa gatcaccttt attcattatt ttttcctttt
     3961 agattgagtt tatttttcaa ttcatcaata ttgtcgacaa caccaatgaa tcccttcgtc
     4021 tcactttgtt aagagtatta gacatacaag ttataccaag cgtgtgaaag ccttgtcaac
     4081 aaaggctttc aattattttc tagattttaa tgtggtaaat ttgattcaag cgaagatttt
     4141 ttagttacag aggtcctctc cacctccatt ttttctttct cttctgagct tatgtttggc
     4201 attttctcca aagcaatttc taaaacttca tcaatccatt tcaccggaat gatggtcaat
     4261 ttttctttaa tattctttgg gatctcaatt aaattcttag aattctcatc aggaataatg
     4321 acagttgaga tacctcctcg atgggcagct agaagttttt cctttaatcc tcctattggt
     4381 aatacttcac ctcttaaggt aatctcgcct gtcatggcca catctgccct cacaggaatt
     4441 tcggtcaaag atgaaaccaa tgcagtacac ataccaaccc ctgcactcgg cccatccttt
     4501 ggtgtagcgc cttctgggac atgtacgtgg agatctactt tttggtaaaa ctcctcatca
     4561 atacccaaac tctttgctct gcttctgaca atagttgttg cagcttggat tgattctttc
     4621 attatatctc caagctgtcc cgtattgatt agcttaccct tacctgggat tacagttgat
     4681 tcaatggtta acaattcacc acccacttgt gtccaagcaa gaccggttac ttgacctatt
     4741 tggttttctt tttctgctat accatatttg aattttctga ctcctagata tttatcaagc
     4801 ccggatggac gaatgtgtgt ggtttttttc tctttcttaa ttgttaaaga cttgaccact
     4861 tttctagcta tttttgcaat atcccgttct aaatttctta ctcctgcttc ccttgtgtaa
     4921 tgccttataa tagaaagaat tgcgctcttt gtgaatttta tctcaccttt tttaaggcca
     4981 ttcatttgca tttgtttatc aaccaaatac ttgtctgcaa tatttaattt ctcatcttct
     5041 gtgtaacctt ctatgcgtat tacttccatt cggtccagaa gaggtggtgg aatattaaga
     5101 gcagtattgg cagtacaaac gaacatcaca tcagataaat caaaatccac ttctagatag
     5161 tggtcattga atgttgaatt ttgctcagga tctagcacct ccaatagtgc cgaagaggga
     5221 tcacctctaa aatccatggt cattttatcc acttcatcga gtaaaaatag aggatttcta
     5281 actccagctt tactgagatt ctgaatgatc ttacctggca tggcaccgat ataagttctc
     5341 ctgtgtcctc taatttctgc ttcgtctcga acaccaccaa gcgacattct tataaatttt
     5401 ctccctgttg aacgagcaat acttgatccc aatgatgttt tacctacacc aggtggtcct
     5461 accaagcaca agattgggcc ttttagtttt ttagttcgtt gctggactgc tagatattct
     5521 gtaattcgct cttttacaga tttcaaccca tagtgatctt cctccaaaat ctgttccgct
     5581 ttatctagtt ttatctttgc ctttgttttt ttcttccaag gtgcatcgat taaagcatcc
     5641 agataattcc tgactaccgt tgcttcagct gacatgggtg acatcatttg tagtttcttt
     5701 aattcagaat ttgctttctt agccgcttct tctgacatac cagatttttt aattttattt
     5761 tcaagatcgt ctaattcatt tgaagcatta tccatatcac ctaattcttt ctgaatggct
     5821 ttcatttgtt catttagata gtattctttc tggctttttt ccatttgatc tttgactcgc
     5881 cctcgaacct tctgttccat ttcaaccata tcaatctcaa catctaccaa tgcaaccaca
     5941 tgttccaatc ttgccttaac atttataagc tctagaactt tctgtttttc ttctaatttc
     6001 aatgacatat gagcaacaat tgtatctaca agtcgccccg gttctttgat attggctaat
     6061 gtgttaacta cttctgctgg aacttttttt gtgattctca cataactctc aacttgtgaa
     6121 agtaatgttc tcctcagggc tttaatttcc ttgtcccctt catggtattt ttcggaaaga
     6181 atacaataat cagcagtgaa ataatcgttg gatctaatct ctaataaatc aacacgtgat
     6241 tcaccctcca ccagtaattt aacagtgcca tctggtaatg taaccaactg cagaatgtta
     6301 gctaatgtac cgacggtatt aatatctttg aaatcggggt catctatgtc tgatcttttt
     6361 tgcgaagata ataagatctt tttttcatga atcattgcct cttcaagagc catttttgac
     6421 ttttctctgc caacaaataa tgggataacc atgtgcggat aaaccacaac atctctcaat
     6481 ggaagcaaag gcttgtcgag aacaatatcg agaaagattt tttcttcttt tttatttggc
     6541 atgaataaat ttccaataat aaattatcac attttatttt atagggataa aaaaagaata
     6601 ttcaacatat caatctcatt taaagagatt tatttagtca gcatttacca acaaagaatc
     6661 atcttctgca tctaattcgg cttgagagtc cttctgatca ccggaggaaa aaactaaaaa
     6721 aggcttagat tgtttttcaa caaaggcttt attcacaacg atttttttag ttttattata
     6781 ggaaggtatt tcatacatag tatcaagcaa tgtatcctcc ataatagttc taagtcctcg
     6841 agccccggtt tttcttctca tggccaattt cgctattgtt tccaatgcct ctgagagaaa
     6901 ttccagttca catttctcca tggcaaacaa cttctgatat tgtttgacga gagcattttt
     6961 tggttcggta agaatcctaa taagagcatg ctcatttagt tcgctcagag aagccactac
     7021 tggcatccgg ccaacaaatt ctgggattag tccatagcgt atgagatctt caggttcaat
     7081 atttttgaaa atttgtccaa tatctttgtt ctcggaagct tctcttaaat cagcaataaa
     7141 accaatgccc gatttgtttg ttctgctctg aataattttg tccaagccgg caaaagcacc
     7201 gccacaaata aataaaatat tagtcgtatc cacttgcaag aactcctgct gaggatgttt
     7261 tcttccgcct tgtggtggca ctgaggcaat agtgccttca atcaacttca gcaacgcttg
     7321 ctgaacgcct tcacctgaaa cgtctctggt gatagaagga ttgtctgatt ttctcgagat
     7381 tttatctatt tcatcaatat atatgatccc tgtttgtgct tgttcaatat cgtagtcaca
     7441 actctggagt agcttttgaa taatattttc tacgtcttct cccacatagc ctgcttcagt
     7501 caacgttgta gcatctgcta ttgcgaaggg tacattcaat attcttgcca aggtttgggc
     7561 caataaggtt ttaccgcaac cggtaggacc tactaataaa atattacttt tgcctatctc
     7621 aacatcctct tcatttcttt tattaggctt attattgagt cttttgtagt gattataaac
     7681 agcaaccgag agcactttct tcgcatcatt ctgatcaata acataatcat caagcatggc
     7741 attaatctct tttggcttat aaagattcaa tccttcacga gaagatgctt cgtttaactc
     7801 ttcatcaata atgttattac ataaatcaac gcactcttcg caaataaaaa ccgagggtcc
     7861 tgcgatcagt tttcgaactt cattttgacc tttgccacaa aatgaacaat acaatatttt
     7921 gctgtcttta ttagtttctt tagttttatc gctcatataa ctagccgttg acctgtgatt
     7981 ttatcagttc gtttcgatta ctgagaatag tatcgatcag tccataatca agcgcattag
     8041 atgcattcat aaaattatca cggtcggtgt ctgatgtaac ttccttgatt gatttgccag
     8101 tgtggctcga taaaatctca ttcactcttt ccctcatact taatatttct tttgcatgga
     8161 tatcgatgtc tgaagcttgg ccttgaaaac ctgaaacagg ttggtgaatc atcatccttg
     8221 aatttggcaa tgaatatctt tttcctttgg caccaccagc caatagtatt gctcccatac
     8281 tggcagcctg accaacacaa atagtgctaa tatctggttt tacaaactgc attgtgtcat
     8341 aaatagacaa ccccgaggta atacttccac caggcgaatt gatatagagt gagatgtcct
     8401 ttttggggtt ttcgctttcc aaaaacagca tttgcgcaac gatcagattg gccatagtat
     8461 cctcaattgg accgaccgca aatataacat tgtccttcaa cagccttgaa taaatatcat
     8521 aagctctctc accccttggg gtttgctcaa cgaccatggg gattaaattt aattcttcat
     8581 ttaattgatt caattcaact ccgatttacc aattctttaa attccatttc ttttttctga
     8641 aactcagttc tttcaatcaa ccagtctatc acttgatctt ccattaccaa tgattttaat
     8701 tggtccatta gttgggacga ttgttcatac attttacgaa tttcttcagg gtttgggtat
     8761 gttttcgata gctcgtctaa tttggttttc actttatctg gatcaagctt gatctccttg
     8821 gactgaacca atttgctaac caaaaggcct aaatggactc tttctttggc aatctcgtta
     8881 aaatcgtcca atggtggagc ctgctcgtga tcttcaatac cagatcgatt catccaatct
     8941 ttttgcatcg catgtgcttc ttgatgaatc ataacttccg ggatctcaat ggcattcttg
     9001 tctttaagaa atttcattaa gttttgtctg atttcattgt taatcctcgc ttccagttcc
     9061 ttggacattg aagatttcac ttcatccatc aactctttct gatcgccact tttaatacca
     9121 aaagattgaa caaatttttg atccaattct gccggtttca attccctcac ttcttttaca
     9181 tcaacggtga aaagcgcttg tttgcccact agttctttct tatgataatt atcaggaaaa
     9241 gtcagttcta tggtctttgt ttcctcagat ttgacaccta tcaaagattc atcaaactca
     9301 ggcagcattt gaccattgcc taactgcata gcaaaatctt ttgccgtgcc tccgtcaaac
     9361 accacaccat caatttttcc ttcaaaatca atgagcagtt gatcgcctcc ttttgagatg
     9421 cgatcgacct tctcccatgt gcccttttga ttctgtaggt tatccatcat ggcattgata
     9481 tctttttggg tgatatcggc atgtggtttt tcgactttta ttttatccaa gcctttgagg
     9541 tcaaactctg ggtaaacttc aatagttgcg gtgtaggaaa aatccttttc tttgacatat
     9601 tcatccagtg caactttagg agggccggct ggttgcaaat ttttctcctg aattgactcc
     9661 atatatgttg tttccaatac atcgcttaag acttcttgca atacttgatc agcatattgc
     9721 cgccgaatga ctttctcagg aaccttaccc ggcctaaagc caggcaattt agcagttttt
     9781 gataattgtg aaaaacgggt atttctcttc tcttcaatca catttgaagg cactgaaata
     9841 tggatatctc tcaccaatcc ttttttagaa ttaactttaa tctttaaatt actcatattt
     9901 tttcttatgt tgctatggtg cgaaaggaga gactcgaact ctcacgggtt accccacagg
//
LOCUS       DASCTH010000026         4276 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000041633, whole genome shotgun sequence.
ACCESSION   DASCTH010000026 DASCTH010000000
VERSION     DASCTH010000026.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000041633"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="QGH35_00575"
     CDS             <1..1035
                     /locus_tag="QGH35_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HJL81058.1"
                     /translation="WKNYSSKELFNELLIAKGMAANKKEATEIQTNMLSKNVGSILRS
                     ATTKEIFQAYLEARFNTNNMLRPFPDGTVLHEQGIMQSLKEGLSSEIPIILGTNRDEN
                     KLFLVNNPRHVRTIFGLPRSRDRVLYDAIAKHRSNTWKYLAVDNPSRELILSGRSNIF
                     AYRFDWDDEPRRLGVDISHLLGAAHAFEIPFVMGNLDKDTLTRYIVGRKNLDSVKQLS
                     EAMMSYWAEFAYTGDPGMGRKGDLPLWKHWSEPNKDAPKYIVFDSDLDRGIRMSTESI
                     SKESLLADIANDEDIKDPKLKCELVDVAIQYDDLEKPEFLDQFNGGQCQQYNPDDWRK
                     TWYESFDEPW"
     gene            1045..1497
                     /locus_tag="QGH35_00580"
     CDS             1045..1497
                     /locus_tag="QGH35_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HJL81059.1"
                     /translation="MQYWLMKSEPDTYSIDDLKSFGVDHWDGIRNYQVRNFFRDQMCL
                     GDQAFFYHSNCKEPGLVGLMEIASKAYPDHTAFDPNEKYFDVKSNPDNPRWLMVDVSY
                     IRHLDRMISLSELREQQQLSEMKLLQRGNRLSVLPLSKDEWDFILSME"
     gene            1585..2163
                     /locus_tag="QGH35_00585"
     CDS             1585..2163
                     /locus_tag="QGH35_00585"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HJL81060.1"
                     /translation="MKRIIIGISGASGVIYGIRMLEILKDIPDIETHLVITSGAKLNV
                     SLETDFEIKDIKGLANVVHNNRNLGASIASGSFQTEAMIVIPCSMRTLSGIANSYASN
                     LIVRAADVILKEKRKLIVVPRETPLHAGHCELLHKISLMGAYVVPPNPAFYNHPESID
                     DIINHTVGRILDLIELDPEIVQRWQGVDVRSE"
     gene            2156..2671
                     /locus_tag="QGH35_00590"
     CDS             2156..2671
                     /locus_tag="QGH35_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJL81061.1"
                     /translation="MSSPDVIKDLFNSIQTFLNEHNTISVATTDNQELSSATVFYVSD
                     EQLNIFFISFPDSKHIQHILNNSEVAATINEDVKDWLEISGLQLKGKVHKVSEQDQDF
                     MLDIYKKKFSYIETLLRAPKTDDERKIADQFRAISVYRLIPDWIRLIDNASGFGSKRE
                     MSFKNKDWELS"
     gene            complement(2681..3469)
                     /locus_tag="QGH35_00595"
     CDS             complement(2681..3469)
                     /locus_tag="QGH35_00595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81062.1"
                     /translation="MERKVAIVTGSATGVGAATAILLAEQDCNVVINYTKSEQEAKKT
                     AKTIKQKGAEVIVCQGDVSNDDDCKAMAKAAVNAWGKIDYLVNNAGKTKFNPYPNLDG
                     LSKDDFLDIYAVNVVGTYQMVRAVEPHMRKLGQGAIVNNSSISGITGIASSIAYAASK
                     AALNMMTKSLAHVLGPEIRINSVAPGMIQTRWLKEGMGDEAYRKMLQQAKNQLPLQEV
                     ATAEDIAEALVWFLEGAKLVTGETLIVDSGIHIGTLPRLSSGDQ"
     gene            3664..>4276
                     /locus_tag="QGH35_00600"
     CDS             3664..>4276
                     /locus_tag="QGH35_00600"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HJL81063.1"
                     /translation="MAFQDMREFLEFLKNKNQLIEVDTELNAARGTTELQPLMRHIHN
                     INGPALMLNNLSGYNHPDIPVLFNPYGTRERTSMILGTEGPLEGKLKFTEVLTNRSLW
                     IKPNMIDRAEATCKQVIIEKNDIDLSQQLPHVWFGKEGPSYITNAVVISKDPETGQLN
                     TGCYRLTQLWNASHPLGEEYSEEEQKKCLSIFAFWNPPGNDIGR"
BASE COUNT         1341 a          757 c          878 g         1300 t
ORIGIN      
        1 tggaaaaatt acagttccaa agaattattt aatgaactat tgattgccaa aggaatggca
       61 gcaaacaaaa aggaagcaac agagatacag acaaatatgc tatcaaaaaa tgtggggagc
      121 attttaagat cagcaactac taaagaaatc tttcaagcct acctagaagc ccgattcaat
      181 accaacaata tgttaagacc gtttcctgat ggaactgtat tgcatgagca ggggatcatg
      241 caatcactca aagaggggtt gtcttcagaa atacctataa ttttaggtac taacagggat
      301 gagaataaat tatttttggt gaataaccca aggcatgttc gaacaatttt tggattacca
      361 cggagtcgag atcgtgtcct ttatgatgca attgctaaac atagatccaa tacttggaag
      421 tatttggcgg tggataatcc ttcgcgggaa ttaattcttt caggaaggag taatattttt
      481 gcatatcgtt ttgattggga cgatgaacca agacggcttg gagtagacat tagtcattta
      541 ctaggggcag cacatgcttt tgagattcca tttgtgatgg gcaacctaga taaagatact
      601 ttgacaagat acattgtggg tcggaaaaac ttggattctg tcaagcaatt atctgaagcg
      661 atgatgtctt actgggctga atttgcctac acaggtgatc cgggaatggg caggaaggga
      721 gatttaccac tttggaagca ttggagtgaa ccaaacaagg atgcccccaa atatattgtt
      781 tttgatagcg atcttgatcg agggatccgt atgtctacag aaagcatttc caaggaatca
      841 ttgttagctg acatcgccaa tgatgaagat attaaagatc ctaagttaaa gtgtgaattg
      901 gtcgatgtcg cgatccaata tgatgatttg gaaaaaccag aatttcttga tcaatttaac
      961 ggtgggcaat gtcaacagta caaccctgat gattggagaa agacttggta tgagagtttt
     1021 gatgagcctt ggtaataagt agtcatgcaa tattggttaa tgaaatcaga acctgacacc
     1081 tatagtattg atgatttgaa atcttttgga gttgatcact gggatggtat cagaaattac
     1141 caggttagaa atttctttag agatcaaatg tgtttgggtg atcaagcttt tttttatcat
     1201 tccaattgca aggagccagg acttgttggg cttatggaaa tagcaagtaa ggcatatccc
     1261 gatcataccg cttttgaccc caatgaaaaa tactttgatg taaaaagcaa tcctgacaat
     1321 cccagatggt taatggtgga tgtaagttac attaggcact tggataggat gatttctcta
     1381 agtgagctta gagagcagca acaattaagt gagatgaaat tgctgcaaag aggaaatcga
     1441 ctttcagttc tacctttgtc taaagatgaa tgggatttta ttttatccat ggaatagtga
     1501 acgtatcata atagcatgct cattttgaac tcgcgttggt ggattaattt tttttgattg
     1561 ttgaaaagat atactatatc gatgatgaaa aggataatta tagggatttc aggtgccagt
     1621 ggtgttattt atggtattcg gatgctggag attcttaagg atattccaga tattgaaaca
     1681 cacttggtta taacatccgg tgctaagtta aatgtctctt tggaaacaga ttttgagatc
     1741 aaagacatta agggacttgc caatgtcgtc cacaacaata gaaatttagg tgcaagtatt
     1801 gcaagcggtt catttcaaac tgaggcaatg atcgttattc cctgctcaat gagaactttg
     1861 tcagggatcg ctaattcgta cgcgagcaat ttgattgtca gagcagcaga cgttattttg
     1921 aaggagaaac gaaaactcat tgtcgtccca agagaaacac ccttacatgc tggacattgt
     1981 gagctcttac ataaaatttc actaatggga gcttatgttg ttccacctaa tcctgctttt
     2041 tataaccatc ctgaatccat tgatgacatt atcaatcaca ccgttggaag aattcttgat
     2101 ctcattgaac ttgatcctga gatcgttcag agatggcagg gggttgatgt aagaagtgag
     2161 tagcccagat gtaataaaag atttattcaa ttcaatccaa accttcctca acgaacacaa
     2221 tacaatatca gtcgcaacaa cagataatca agaattgtct tccgcgactg ttttctacgt
     2281 ttctgatgag caattaaata ttttttttat ttcctttcct gattcaaaac atattcaaca
     2341 catcctaaac aattcagaag tggcagccac tatcaatgaa gatgttaaag actggctgga
     2401 gattagtgga ctacaactga agggaaaagt tcacaaagtt agcgaacaag accaagattt
     2461 tatgttggat atctataaaa agaaattcag ttatatagaa acgcttcttc gggctcctaa
     2521 gacagatgat gaaagaaaaa tcgcagatca atttagagct atcagtgtat accgtcttat
     2581 acctgattgg atcagattaa tagataatgc ttcaggtttt ggttcaaaac gagagatgag
     2641 ctttaaaaac aaggattggg aactatctta gcccactgat ttattgatct ccacttgaaa
     2701 gtctgggtaa cgttccaata tggataccac tgtctacaat tagtgtttca ccagtaacta
     2761 atttagctcc ctctaaaaac cagaccaggg cttcggcaat gtcttctgct gttgcaactt
     2821 cttgtaaagg tagttggttt tttgcttgct gcagcatttt ccgataagct tcatcaccca
     2881 taccttcttt gagccaacgc gtctgaatca ttccaggagc aacactattg attctaattt
     2941 cagggccaag gacgtgtgcc agagattttg tcatcatgtt caaagctgct ttggatgcag
     3001 cataggcaat tgaggaggcg attccagtaa taccacttat tgaagaatta ttgacaatgg
     3061 caccttggcc taattttctc atatggggct ccaccgctct gaccatctga taagttccga
     3121 ctacattgac agcatagata tctagaaaat catctttgct gagaccatct aaattgggat
     3181 aagggttgaa ctttgttttg ccggcattat ttaccaaata gtcgattttt ccccaagcat
     3241 taacagctgc ttttgccatt gctttgcaat catcatcgtt cgaaacatca ccttggcaaa
     3301 ctatgacttc tgctcctttc tgcttgatcg ttttagcagt ttttttagct tcctgttcac
     3361 tctttgtata gttgatgaca acattgcaat cttgttctgc aagcaagatt gcagtggcag
     3421 ctccaacacc agtggctgaa cctgtaacaa ttgcgacttt tcgttccatt tcagttaacc
     3481 ctcacgtttc attttttttc ttaaaagtag attatttatg tagaaactct cacggctcct
     3541 aatattacac atttccgttg gttattatcg atattatagg cacaattaac agtaacgaat
     3601 ttatttttca tagattatag gtcttatgat tgatggtttt attggcgaaa taggagatga
     3661 caaatggctt ttcaagacat gagagagttt ttagagtttc tcaaaaataa aaatcaatta
     3721 attgaagtgg atacagaatt gaatgcggca aggggaacca cagaattgca accgttaatg
     3781 agacatattc acaacatcaa tggacctgct ttgatgctta ataatttatc tggttacaat
     3841 cacccagata ttcctgtttt atttaatcca tatgggacac gcgaaagaac atctatgatt
     3901 ctaggtacgg agggaccttt agagggtaag ctgaaattta ctgaagtgtt aaccaatcga
     3961 tctttatgga taaaaccaaa catgattgat agagcagagg caacatgtaa acaagttata
     4021 attgagaaga atgacataga ccttagtcaa caactcccac acgtgtggtt tgggaaagag
     4081 ggtccttcgt atataaccaa tgccgttgtt atttcgaaag atcctgaaac cggtcagctc
     4141 aatactggct gttacagact cacccaacta tggaatgcat ctcatccact cggtgaggag
     4201 tattcagagg aagaacagaa aaaatgtcta tcaatttttg ctttctggaa tcctccagga
     4261 aatgatattg gacgcc
//
LOCUS       DASCTH010000027         2241 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000044273, whole genome shotgun sequence.
ACCESSION   DASCTH010000027 DASCTH010000000
VERSION     DASCTH010000027.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000044273"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            102..1292
                     /locus_tag="QGH35_00605"
     CDS             102..1292
                     /locus_tag="QGH35_00605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HJL81064.1"
                     /translation="MNSFFASIEQLDQPELFGRPVAVTNGNQGTCIITCSYEARYWGI
                     KTGMRLKQAKQLCPELIQRPSRPKRYAQISTKIMHGLKTITPDIEVYSVDEAFLDMTH
                     CQKIIESPEKVAKSIKKLILELSGLSCSIGISGDKTTAKYAAKQEKPDGLTIIPPEKA
                     ENRLKNAPVTDLCGIAKGIGRFLNSYGVYTCGDMKNIPISILGQHFGNPGRRIWLMAQ
                     GQDPENIKTDVAEPKSIGHGKVMPPSTRNKKTILVYLQHMSEKVGSRLRRHDLQTSKF
                     FIGILSQHGWIGGKMKTEYPTNDGQKIMDLCEQLLNKQWHGEGINQIQVTAIHPKQSN
                     QQLDFLENKKQWSSAHEKNSVMDAINQRYGDLTIAPSNLIHRSSMPDVIAPAWKPYGH
                     RRTI"
     gene            1270..1704
                     /locus_tag="QGH35_00610"
     CDS             1270..1704
                     /locus_tag="QGH35_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81065.1"
                     /translation="MAIGELFNMCGGVTYTHKSDHMRVYFPNPKAKLPVLNKNNKTSL
                     ILWGRRKGQPGKLPMGGWARLDSIYSGIWDRWFPKAVKIPVHSFMEKDHEGKSHWFDL
                     IKGQWIQGLIAVEKQEQRLYVVTIEPELEHSIHQRWPRIMSG"
     gene            complement(1921..>2241)
                     /locus_tag="QGH35_00615"
     CDS             complement(1921..>2241)
                     /locus_tag="QGH35_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012774.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HJL81066.1"
                     /translation="QYCKRSNGKIWACGKKATQKLKELINGQEVQCTDEGKDKYDRTL
                     SICYANGVDLQAEMVRSGMAMAYLKYSNRYANEMVEAMTNRAGIWSGDFTDPDLWRKQ
                     SRRK"
BASE COUNT          743 a          442 c          420 g          636 t
ORIGIN      
        1 agcccagcgt atccaaattc agggagtcat cgttgggcaa cttagaagct accagtaata
       61 agcattcatt accttggcct cgaatgatcg tattggttga tatgaattca ttctttgcat
      121 caatcgaaca acttgatcag ccagaattat ttggacgccc cgttgccgtt accaatggaa
      181 accaaggaac ctgcatcatt acttgctctt atgaggctag gtattggggt attaaaacag
      241 gtatgcgtct caaacaagca aaacaactat gtcctgaatt aatccaaaga ccttctagac
      301 caaaacgtta tgcacaaata tctaccaaaa ttatgcatgg tctaaaaact atcaccccag
      361 atatcgaagt gtattccgtc gatgaggcct ttcttgatat gacacattgc caaaaaatca
      421 ttgagtcgcc agaaaaagtg gccaagagca ttaagaaatt aattcttgaa ctatcaggtt
      481 tatcttgctc catcggtatt agtggtgaca aaacgacagc aaaatatgct gccaaacaag
      541 agaagcctga tggcctaacc attatccccc cagaaaaagc agaaaacagg ctaaaaaatg
      601 ctcctgtaac tgatttgtgt ggcatagcaa aaggtatagg cagatttcta aatagttatg
      661 gagtctatac ttgtggtgat atgaaaaata ttcccatcag tattctaggt cagcatttcg
      721 gcaatccagg gcgtcgtatt tggcttatgg ctcaaggcca agatcctgaa aatattaaga
      781 ctgatgttgc cgaacccaaa tcaattggac atggcaaagt catgccaccc agtaccagaa
      841 ataaaaaaac aatcctagtg tatctgcaac atatgagtga aaaagttggc agccgcctga
      901 ggcgtcatga tttacaaact tctaaatttt ttattggcat tctttcacag cacggatgga
      961 ttggtgggaa aatgaaaact gaatatccaa caaatgatgg tcaaaaaatt atggatttat
     1021 gtgaacagct tcttaacaag caatggcatg gtgaaggcat caaccaaatt caggtgacag
     1081 caatacatcc aaaacaatcg aatcaacagc tcgatttcct tgaaaacaaa aaacagtggt
     1141 catctgctca tgaaaaaaat agtgttatgg atgcaattaa tcagcgttac ggcgacctga
     1201 ccatagcccc aagcaaccta attcatcgat ccagtatgcc agacgtcatt gcccctgcgt
     1261 ggaaacctta tggccatagg cgaactattt aatatgtgtg gtggagtaac atatacacac
     1321 aaaagcgatc atatgagagt gtattttcca aaccctaaag caaagttacc tgttttaaat
     1381 aaaaacaata aaacatcctt gatattatgg ggaagaagaa aaggacaacc tggaaaatta
     1441 cccatgggtg gatgggcaag gctggattca atttattctg gtatctggga caggtggttt
     1501 ccaaaggcag ttaaaatacc ggtacatagt ttcatggaga aagatcatga gggcaaaagt
     1561 cattggtttg atttaatcaa aggacaatgg attcaaggat taatagctgt tgagaaacaa
     1621 gaacaaagat tatatgttgt caccatagaa cctgaattag aacacagtat ccaccaacgt
     1681 tggccaagaa taatgagtgg gtagtattaa ataactaaga gtgcttatgg atcttgcaat
     1741 cagcttaatg attatcaatt tggtattgtc ttattggaca ggtctgactg cgaaaagaaa
     1801 gggtaaaagt ttcacacttt ggtgcgtttt acagctatta ttttatttcc ccatcttact
     1861 aattgcattc cactctcctg ataaaaaata aaaactggaa gtaagtatta tatttattaa
     1921 ctatttcctt ctactctgtt ttcgccaaag atcaggatca gtaaaatcac cactccaaat
     1981 acctgctctg tttgtcatgg cttcaaccat ttcatttgcg tatctatttg aatacttcaa
     2041 ataagccata gccattcctg atcgaaccat ttctgcctgt aaatcgacac catttgcata
     2101 acaaatactg agtgttcgat catacttatc tttaccttcg tcagtgcatt gaacctcttg
     2161 cccattgatt aattctttaa gtttttgagt ggcctttttt ccacaagccc atatctttcc
     2221 attacttctt ttgcaatatt g
//
LOCUS       DASCTH010000028         2724 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000044625, whole genome shotgun sequence.
ACCESSION   DASCTH010000028 DASCTH010000000
VERSION     DASCTH010000028.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000044625"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="QGH35_00620"
     CDS             complement(<1..643)
                     /locus_tag="QGH35_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81067.1"
                     /translation="MNRFLISFVSIYLLVFVSSLNGQEPLSRIHCGQMLDIKNSLVRN
                     NVMISIDQGSGKIKSLDFDYSKTDYDDIDTLNLSNYFCLPGLIDMHTHISEDHYRPLV
                     DYLTMTQLEQIAIGRGLVKKTLLAGFTSVRDLGVYIAWTDKLLRDEINAGLTDGPRMQ
                     ISGFYLTIPGGGGDIDVPGYTGNVPAHIRQGVTQGVENFRNRAETAVDGGADVL"
     gene            718..1677
                     /locus_tag="QGH35_00625"
     CDS             718..1677
                     /locus_tag="QGH35_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81068.1"
                     /translation="MSDGEKTLPIVIATSVVRGSQQGESHGGIYLVDFEKQDAKIMVD
                     WNESNIDFSGRGWDRGLRGIEFYGDEIFIAASDELFVYDQTFDIKRSYKNPFLKHAHE
                     IYKFKNLLFVTSTGFDSLLVFDLERDEFTRGIHLRQNPKGWKTQLFDPQSEDGPPMGN
                     NLHLNNVQCNKKGLFTSGLRTNALIYMDSDLNAREYCSLPQGAHNARIFQHGILFNDT
                     KSDCMRSIGRDGNELNFKIPSYKEEELTHTNFDDSRIARQGFARGLCVIRKNLVAVGS
                     SPSTISIFDLENKKRIASVNLSMDIRNAIHGLEVWPYERLLDS"
     gene            1655..1939
                     /locus_tag="QGH35_00630"
     CDS             1655..1939
                     /locus_tag="QGH35_00630"
                     /inference="COORDINATES: protein motif:HMM:NF018717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HJL81069.1"
                     /translation="MKGYWIVRCQYNDQDAFEEYASLATKIVADNGGKFLIRGGQQLQ
                     MENGSYERTVMVEFPNLELAKEIYHSQEYQEAYNIIASSAERDFVIVEGL"
     gene            complement(1968..>2724)
                     /locus_tag="QGH35_00635"
     CDS             complement(1968..>2724)
                     /locus_tag="QGH35_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016274.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HJL81070.1"
                     /translation="RDSGPNFLINDSGLLAGSTWEFNAWGDKFRPYDQDALMGSRILD
                     WVSVDEYISTMIAEGGGITVDGEGTVITTESCFPNKNRNPHLTKKEIESELCRTLGAS
                     KVIWIPGDPDETGTDGHIDGIAAFIEPGRILVEISPDSSDPHYPVGQNNVKALKNIKD
                     AKGRALEIDFIYEGDYSVLEFDECRSYINSYLANGAVIVPGYNHERDHLAVETYQKIY
                     PDREVVQIQISDIAIGGGGIHCITQQQPNIINY"
BASE COUNT          849 a          539 c          519 g          817 t
ORIGIN      
        1 tcaacacatc tgcccctcca tcgacagctg tttccgctct gttcctaaaa ttttccactc
       61 cctgtgttac accttgtcga atgtgagctg gaacgttgcc tgtataacca ggaacatcga
      121 tatcaccacc gccacctggg atggtcaagt agaaacccga aatttgcatt cttggaccat
      181 cagttaatcc ggcgttgatc tcatctcgta ataatttatc agtccaagca atatagactc
      241 ccaaatcgcg aactgaagtg aatccggcta gtaatgtctt tttcacaaga cctcttccaa
      301 tagcgatctg ttccagctgc gtcatagtta gataatcaac caatggtcta taatggtctt
      361 ccgatatatg ggtgtgcata tcgatcagac ctggtagaca aaaataattt gataggttaa
      421 gtgtgtctat atcatcataa tctgtcttag agtaatcgaa atcgagggac tttatttttc
      481 cagaaccctg atcaatgcta atcattacat tgtttcgtac caagctattt ttaatatcga
      541 gcatttggcc acaatgtatt ctagacaaag gctcttggcc atttaggctg ctgacaaaga
      601 cgagaagata gatggaaaca aagctgatca agaatctatt catgagcaac accttagcat
      661 agaaataagt tcatttatgg gtatttgctg tgttcaattt tccattacaa tatagagatg
      721 agtgacggtg aaaaaacatt gccaattgtc atagcaacat ctgtagttcg tggtagccaa
      781 caaggtgaga gccatggtgg tatctatctt gtagattttg agaagcaaga tgcaaaaatt
      841 atggttgact ggaatgaatc caatattgat ttcagtggca gaggatggga tcggggttta
      901 cgtggaatag aattttatgg ggatgaaata tttatcgcag ccagcgatga actttttgtt
      961 tacgatcaaa catttgatat caaacgttct tataaaaatc cttttttaaa acatgcccac
     1021 gaaatatata aatttaagaa tctcttattt gtcacttcaa ccggttttga cagtcttttg
     1081 gtgtttgatc tggaacggga cgaatttaca agaggtattc acttaagaca aaatccaaaa
     1141 ggctggaaaa cgcagttatt tgacccacaa tctgaagatg gacctccgat gggtaataat
     1201 ttgcatctga ataacgtgca atgtaacaaa aaaggacttt ttaccagcgg gttaagaacc
     1261 aatgccctca tctatatgga cagtgatttg aatgcacgtg agtattgttc actgccccaa
     1321 ggagcacata atgcaagaat tttccaacat gggatcttat tcaatgatac taaaagcgac
     1381 tgcatgcgat ctattggcag agatggtaat gaattgaatt tcaaaatccc tagctacaaa
     1441 gaagaagaat tgacccatac aaattttgat gacagtcgga ttgcacgaca gggatttgca
     1501 agaggtttgt gcgtgatcag aaaaaattta gttgctgtgg gatcttcgcc ttcaacaatt
     1561 agcatctttg atttagaaaa taaaaaacga atagcgagtg ttaacctaag catggatatt
     1621 agaaacgcaa ttcacggttt agaagtttgg ccctatgaaa ggttactgga tagttagatg
     1681 ccaatacaat gatcaggatg catttgaaga atatgcgtct ttagccacta agatagtggc
     1741 ggataatggt ggtaaatttc tgatcagagg cgggcaacaa ctacaaatgg aaaatggcag
     1801 ttatgaaaga accgtcatgg tagaatttcc caacctagaa ctcgccaaag aaatctatca
     1861 cagtcaagaa tatcaggaag catataatat tattgcatct tccgctgaaa gagattttgt
     1921 aattgtagaa ggcttataaa aaagctttcc caacagacca tccactatca ataatttatg
     1981 atattaggtt gttgttgggt tatgcagtgg ataccacctc caccaatcgc aatatctgaa
     2041 atttgtattt gtaccacctc tctatcggga taaattttct gataagtttc tactgcaaga
     2101 tgatctcgct cgtggttata gccaggaaca attactgctc catttgccaa ataagaattg
     2161 atgtaggatc tgcattcgtc aaactcaaga actgaataat ctccctcata aataaaatct
     2221 atttccaaag ctctaccttt tgcatcttta atatttttca gtgctttcac attgttctgt
     2281 cccactgggt agtgtggatc tgaagaatca gggctaatct caaccaatat ccttccaggc
     2341 tcaataaagg ctgcaatccc atctatatgt ccgtctgtcc ccgtttcatc tggatctcct
     2401 ggaatccaga taaccttgga ggctcccaaa gttctacaaa gttcagattc tatttccttc
     2461 ttggtcaaat gtggatttct atttttgttt gggaaacagg attctgtggt tataacagta
     2521 ccttcaccat caactgttat cccccctcct tctgcaatca tcgttgatat atactcatca
     2581 acactcaccc aatcaagtat tcgagatccc atcaaagcat cttgatcata gggtctaaat
     2641 ttatctcccc aagcattaaa ttcccatgtc gaaccagcta gaagtcctga atcatttata
     2701 agaaaatttg gtcctgaatc acga
//
LOCUS       DASCTH010000029         2689 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000049702, whole genome shotgun sequence.
ACCESSION   DASCTH010000029 DASCTH010000000
VERSION     DASCTH010000029.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000049702"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="QGH35_00640"
     CDS             <1..459
                     /locus_tag="QGH35_00640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81071.1"
                     /translation="LFSILFWFSFIGKFTFGAISDKIPKRKVLLLASIILFIGSLLLF
                     DIGQNHISLTQNTTKLTLFTVIFGLGFGGSFSMIQLVAVEIFGKAYLGRILGIITFVD
                     GMGAALGTNLLSQIKTNSGSYLIPFMIVTGVALIAIINVFFIKPIKPSAN"
     gene            508..1356
                     /locus_tag="QGH35_00645"
     CDS             508..1356
                     /locus_tag="QGH35_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014698.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HJL81072.1"
                     /translation="MKFQTFDIEPLSSVLGAEIHNVDISKSLSKETLKEIKEAWLEYQ
                     VLFFRDQDLSPEEHMAFAKNFGELQGPGYMPTHPDFPNVYIQEYPGLYGGIVTDVDWH
                     IDASFMEKPLKGAVLHALDVPETGGDTAWINLYLAFDSLSKPMQMFCSKLTAVHDNFH
                     KNLNNVLEKYGSKGYEMARKATPPQQHPLVCKHPETGRKCLYYSELLVSHFKELTEEE
                     NKGLINFLKRHITKPEFYYRFQWENNSVAFWDNRCTAHRGIFDFGQSHRLMHRVAIQG
                     ESVPSK"
     gene            1369..>2689
                     /locus_tag="QGH35_00650"
     CDS             1369..>2689
                     /locus_tag="QGH35_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81073.1"
                     /translation="MWIKKFHNDNQEDKRSPIPTQVVSNEEFLPRSQTQQQKQVENLI
                     QNLAQQYGRKVGLNRRDFLKTSSGMALAFMAMNKVFGQYFDVHAEEMLDTSVHAELWP
                     KNEFIFDVQTHHVATGKNEPLGFRIMAWPFNEELRGKKPKKGDLAFNNYVKEIFLDSE
                     VTVACLSGVASKVLDVINVDEMVAARNTVNAMSGSQRMLSHGPIAPYIPNFLEEAERQ
                     ATDLKVDAWKFYTGVFNKFGEYPWWMDDEDKLYPFYDKIKQWGVNTVCVHKGLPFVRR
                     LKPGQPDYSRPRDIKKVALDHPELNIVIYHSGFKDDNPNLAPEDEYLDENAYLPWTTD
                     LCQDRINNPSMTNVYMELGTTFGHTVITHPKICAHLLGQIIQAFGSDHVLFGTDSIWW
                     GSPQWQIEALRRFQIPLEMQEKFGYAAITEDDKKKILGLNAAKLYQID"
BASE COUNT          867 a          495 c          540 g          787 t
ORIGIN      
        1 ttattttcaa tccttttttg gtttagcttc attggaaaat ttacatttgg agcaatcagc
       61 gacaaaattc caaaaagaaa agtgcttcta ctagcctcaa taatcctatt tataggctct
      121 ttattgctat ttgatatcgg tcaaaatcat atttcactta ctcaaaacac aacaaaatta
      181 acactattca cagtgatttt tggattagga tttggaggta gctttagcat gattcagcta
      241 gtagcggttg agatatttgg caaggcctac cttggaagaa ttcttggaat cattacattt
      301 gttgacggta tgggagccgc tctaggcact aatcttttga gccagattaa aactaactct
      361 ggcagttatt taataccatt tatgattgtt acaggcgtcg cattgattgc gatcatcaat
      421 gtatttttca ttaaacccat taaaccaagt gccaattagt cttatttgtg ttgtatagtt
      481 ttagaagcta gtctagaaga cagtgttatg aagttccaaa catttgacat cgaaccatta
      541 tcatcagtgc tgggtgcaga aatacataat gtagacatca gtaaaagcct ctccaaagaa
      601 acactcaaag aaatcaaaga ggcttggcta gaataccaag ttcttttttt tagagatcaa
      661 gacctgagcc cagaagaaca tatggctttt gcaaaaaact ttggagaact tcaggggcct
      721 ggttacatgc ccactcatcc agattttccc aatgtttata ttcaggaata cccaggattg
      781 tacgggggaa tcgtcacgga tgtggattgg cacatcgatg cctcttttat ggaaaaacca
      841 ctcaaaggag cagtgctaca cgctttagat gtacctgaaa ctggcggaga tacagcttgg
      901 attaatttat atttagcatt tgattctctt tcaaaaccga tgcaaatgtt ttgctccaaa
      961 ctgactgcgg tgcatgataa ctttcataaa aatctcaaca atgttctcga aaaatatgga
     1021 tccaaaggct atgagatggc tagaaaagca acccctcctc aacaacatcc attggtttgc
     1081 aaacaccctg agacaggaag aaaatgtctg tactatagtg agttgctggt ttcacacttt
     1141 aaagaattga ccgaagagga aaataaggga ttgataaatt tcttaaaacg gcacataaca
     1201 aaacctgagt tttattatcg gtttcaatgg gaaaataatt cagttgcctt ctgggataat
     1261 cgatgcactg ctcatagagg tatatttgat tttggccaat ctcatcgttt aatgcatcga
     1321 gtggccattc aaggtgaatc tgtgccatca aaataaagga tataagatat gtggattaaa
     1381 aaatttcata acgacaatca agaggacaaa cgctctccta ttccaacaca agtggtatct
     1441 aatgaggagt ttctgcccag atcacaaact caacaacaga aacaagtgga gaatttaatt
     1501 caaaacttag cacaacaata tggaagaaaa gtgggtttaa accgtcgaga ttttttgaaa
     1561 acatccagtg gcatggcgct tgcctttatg gccatgaaca aagtctttgg tcaatacttt
     1621 gatgttcatg cagaagaaat gctggatact tccgttcatg ctgagctttg gcctaaaaat
     1681 gaatttatct ttgatgtgca aacacaccac gtggcaacgg gtaaaaatga acctttgggc
     1741 tttaggataa tggcttggcc attcaatgaa gaattacgag gcaaaaaacc aaaaaaggga
     1801 gatttggcat ttaataatta tgttaaggaa atttttctag acagcgaggt tacagttgcc
     1861 tgtctgagtg gtgtagcatc aaaagtcctt gatgtcatca atgtcgatga aatggttgca
     1921 gctagaaata ctgtcaatgc catgtcggga tcacaaagaa tgctaagcca tggacctatt
     1981 gctccctata ttcccaattt tcttgaagaa gccgaacgtc aagcgactga tcttaaggtt
     2041 gatgcctgga aattttatac aggagtattt aataaatttg gagaatatcc ttggtggatg
     2101 gatgatgaag ataagctcta tcctttttat gacaagatca aacaatgggg tgtcaacaca
     2161 gtctgtgtgc acaaaggtct tccttttgtt cgacgtttaa aacctggaca accggattac
     2221 agccgtccaa gagatataaa aaaagttgca ttagatcatc ctgagctcaa tattgttatc
     2281 taccattctg gatttaaaga cgataatcca aatctggcac ctgaagatga atacttggac
     2341 gagaacgcct acctcccttg gactacagat ttgtgtcagg atcggatcaa taaccctagt
     2401 atgaccaatg tctatatgga gctaggcact acatttggac atacggttat tactcaccca
     2461 aaaatttgtg ctcatttact gggacaaatt attcaggcat ttggatcgga tcatgtttta
     2521 tttggcacag actccatttg gtggggctca cctcaatggc aaattgaagc actaaggcga
     2581 tttcaaatac ctctggagat gcaagaaaaa tttggctatg ccgccattac tgaggatgat
     2641 aaaaagaaaa tcctaggttt aaatgcagca aagctgtacc agattgatg
//
LOCUS       DASCTH010000030        10656 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000049749, whole genome shotgun sequence.
ACCESSION   DASCTH010000030 DASCTH010000000
VERSION     DASCTH010000030.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10656)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10656)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10656
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000049749"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            214..1404
                     /locus_tag="QGH35_00655"
     CDS             214..1404
                     /locus_tag="QGH35_00655"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012141454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HJL81074.1"
                     /translation="MRNSILIILATFTASSFSHNGDFHLSENCPSSFENVNGHCEYRS
                     LYHQYQSLENKGVGGLRTSPPKVRDGFSPQQIDLGRYLFFDPLLSGNHSLSCASCHHP
                     NLGFSDARARSIGVNALDVGRASPTLWNVGFLTTFFWDARTHSLEDQMIGPLFAEKEM
                     ANTPENLVQEINANQIYRTLFAKAYPGTDKELITLNQIYTSIAAFEASLISLNSRYDQ
                     YAHGHHQALTLNEIQGLNIFRSFVARCAECHTPPLFTNQQVAVLGTPEPQGRPLDPGA
                     GKIFDSLKLRGGFKVPTLRNINQTAPYMHSGAFTNLRDAVEFYTKGRGHAVPEGEELL
                     LHWHIWEPELTDNEIDLLVDFLLTLTDESGKPQVPESVPSGLPVVSVETMQLNQSAHA
                     IGEK"
     gene            1404..2924
                     /locus_tag="QGH35_00660"
     CDS             1404..2924
                     /locus_tag="QGH35_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015046868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HJL81075.1"
                     /translation="MNQSRKWAVYFFIVVVFAAGFLIGKLNVDGSKTLIGDVDKYQTS
                     IQARNDSETQYGSVISIEREPFNGQIHTIEQGESIQDAVNDAQPGDVIRILPGTYKET
                     VYIDKDNITLTGVIIKGEWPTLEGNYELNDAILYSGNNFTVENLKITHYKGNGIMGQA
                     GNNFIIRNNIILDSGVYGIFPEFGKNGIIEYNVLTGTEDAAIYVGMCDNVHVAHNEVF
                     ANVAGIEIENTRHAIVENNYAHNNTGGILVFITPGLPIKTTYDVIVRDNYVVDNNHEN
                     FGAPGSIVAGIPKGTGILIMAGDDVTLQNNVITGNNNAGIIITDHGNAPNLTLDPEVE
                     PNSDRIAILDNLMYDNGADPSELVKALMLTKMTTRGPDIIRVGESKDSCILNREKYLS
                     FGIDEFGTCAFTSTYGTKTYLLEKPVVMQAINAEERGKLTYYSVCTGCHAYNVRMVGP
                     PTQEIQALYSDNPQGIVDYISNPTRKRADYPEMPPQNYLSMELRMAAAQFMLDAEQ"
     gene            3146..5035
                     /locus_tag="QGH35_00665"
     CDS             3146..5035
                     /locus_tag="QGH35_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007234427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJL81076.1"
                     /translation="MSKQITRRDFCNGIAIGTGISLLSTMDLFGQNAFTIQNPEQPFA
                     YYPPTLTGMRGSHKGSFEVAHALAWTGEKPAKYEKLDEEYDLVIVGAGISGLATAWFY
                     QQKMGQDARILILDNHDDFGGHAKRNEFNHDGKLLLGIGGSVNLENPRDYSEEAKGLL
                     HDLGIDLDAMRNNINDDHYALANPESNHALALPGPNGHVTVKANWIFLYQGEGDIETA
                     IKSLPLPVIEQEKLIEFLSGERDYLDDLSLREKYNYVQTVSYSRFLSDRVGLDEETSS
                     IFYAWMKLIYCVDGRNVSVLEALLVGAPGMQGMGRLAKFIQSLGSLSIDNNESLYFPD
                     GNASIPRLLVKKLIPAVTSSEANFNNISTSHFDYSLLDRSDHSIRLRLNSTVVSVRQN
                     KDDGVDVDYVKDGKALRVTGKHSVLACYNEMIPHLCPELPESQKDGLRYGEKAPLVWA
                     NVLLENGDAFSELGVNLVTCPKDPFVVVTTAPPTSTGEHQPPKDPSDPMVVFMMSIPS
                     VKSTGKETARELFKVGRQVVYSTSFATYEKQVRDQLQVLLGPHGFNHEVDIKAITVNR
                     WPHGYAYEYFALDDPSWEEGQAPHEIGRTQFGNISIANSDSQALAYLDAAIDAAWRAI
                     EEQTA"
     gene            5194..6123
                     /locus_tag="QGH35_00670"
     CDS             5194..6123
                     /locus_tag="QGH35_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJL81077.1"
                     /translation="MINKLRLSFLASVIFFISSIFFIPLQSSEIKTQLILLGTGTPNA
                     EPQRSGPSLAVVVGDNSYIVDFGPGIVRRATALSKQWEGSFQALDPENLKIAFLTHLH
                     SDHTGGYPDLILTPWVLGRETPLEVYGPKGLSKMTELIIEAYKQDIDYRLEGLEPANQ
                     YGWMVNVYEIEEGIVYRDELVEVTAFKVNHGSWDNAFGYKFVTPDKTIVISGDTGPSG
                     NLINYSKEADILVHEVYSQAGFEKKDEVWKKYHSTHHTSTYELGEIANIIKPGLLVLT
                     HVLFWGSTEKEILDEIHEVYNGPVSFGSDLIVY"
     gene            6140..6943
                     /locus_tag="QGH35_00675"
     CDS             6140..6943
                     /locus_tag="QGH35_00675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81078.1"
                     /translation="MTPSNNKKTVLITGASAGIGRAFAEVWAEAGFNLLLTARREDRL
                     QEIAHQLQSEQDVQVNIIPMDLADPEAPKKIHQHCLDNNIHIDALINNAGYGMSGEFE
                     QMNWNSHQDFLQVMVNVVVQLTYLFLPGMIQKKYGRIINLASMAPFMPSPSMAGLYSP
                     VKIFQIKFSESIQIQYFDKGIYCSAVCPGLTRSEFHEAAGMHEVVNNSPNWLWMDSRT
                     VAQQGFDAVMKGKTLIINGTLNKAFVILAKFIPDKVTRSVAKYLSKRSY"
     gene            6946..7620
                     /locus_tag="QGH35_00680"
     CDS             6946..7620
                     /locus_tag="QGH35_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012774.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HJL81079.1"
                     /translation="MHNGIKTTFLLQLMAGLILVLPVYGQQDSGWVNGSNISESINET
                     SPIGLVDMPSGTAEDFEDDTLVDSKFLDKQKDQNRDFFITGKARIIDGDTISINKTKI
                     RFSGIDAPESYYYGQTQYCKRSNGKIWACGKKATQKLKELINGQEVQCTDEGKDKYDR
                     TLSICYANGMDLQAEMVRSGMAMAYLKYSNRYENEMIEAMTNRVGIWSGDFIDPDLWR
                     QQNRKK"
     gene            complement(7837..8271)
                     /locus_tag="QGH35_00685"
     CDS             complement(7837..8271)
                     /locus_tag="QGH35_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81080.1"
                     /translation="MAIGELFNMCGGATYTHKGDHMRVYFPNPKAKLPVLNKNNETSL
                     ILWGRRKGQAGKLPMGGWARLDSIHSGIWDRWFPKAIKIPVHSFMEKDHEGTSHWFDL
                     VKGQWIQGLIAVEKQEQRLYVVTIEPELEHSIHQRWPRVMSG"
     gene            complement(8249..9508)
                     /locus_tag="QGH35_00690"
     CDS             complement(8249..9508)
                     /locus_tag="QGH35_00690"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007146339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HJL81081.1"
                     /translation="MGNLEATSNKNSLPWPRMIVLVDMNSFFASIEQLDQPELFGRPV
                     AVTNGNQGTCIITCSYEARYWGIRTGMRLKQAKQLCPELIQRPSRPKRYAQISTKIMH
                     GLKTITPDIEVYSVDEAFLDMTHCQKIIESPEKVAKSIKKLIFELSDLSCSIGISGDK
                     TTAKYAAKQEKPDGLTIIPPGEAENMLKNAPVTDLCGIAKGIGRFLNSYGVYTCGDMK
                     NIPISILGQHFGNPGRRIWLMAQGKDPESIKTNVAGPKSIGHGKVMPPNTRNKKIILV
                     YLQHMSEKVGSRLRRHNLQTSKFFIGILSQHGWIGGKMQTEYPTNDGQKIMDLCQQLL
                     DKQWHGEGINQIQVTAIHPKQSNQQLDFLENKKQWSSSHEKNSVMDAINQRYGDLTIA
                     PSNLIHRSSMPDVIAPAWKPYGHRRTI"
     gene            complement(9483..10091)
                     /gene="lexA"
                     /locus_tag="QGH35_00695"
     CDS             complement(9483..10091)
                     /gene="lexA"
                     /locus_tag="QGH35_00695"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HJL81082.1"
                     /translation="MLTPSETRTLKFIQKYFVKSGYAPTLQEIAQGTGIKSRGVIHRY
                     LRALQSSDLITILPNRKRGIRLNLEANENALAAPIIPLLGRIAAGQPIEAIEGRDELN
                     LADFLMNADRFALQVTGNSMIEAGILDGDTVIVKHQQTANNGDIVVALIDGLEATLKR
                     LKKLPDGMILLMPENKFIEPRVYPAQRIQIQGVVVGQLRSYQ"
     gene            10256..10495
                     /locus_tag="QGH35_00700"
     CDS             10256..10495
                     /locus_tag="QGH35_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81083.1"
                     /translation="MLPLELIIRALSIIFGLIIFILAIIFGWFIFSILVGVLFFIWII
                     FYVRSLWNRRREKTGANDAIIDAEYTVIDVEDSDD"
BASE COUNT         3223 a         1966 c         2232 g         3235 t
ORIGIN      
        1 ggttaggcag catatattca attggatgag tcttgtcggc tttgtaattt ctagtgtttt
       61 tttagtgata ttttttatca atgctttgct attcagaatc ttaagaaaat cttgatcagt
      121 attaattgat aaacaattct ggataaatgt agccgtttaa ttttattaat ctagttaact
      181 tgatagcggt tttgtctgag ttagaataaa ggtatgagga attctatctt aattatttta
      241 gccactttta cggcatcttc tttttctcac aacggagatt ttcatctatc agaaaactgc
      301 ccatcgagct ttgagaacgt caacgggcat tgcgaatatc gcagtctata ccatcaatac
      361 cagtctctgg agaacaaagg tgtgggtgga ctaagaacct caccaccaaa agtacgcgat
      421 ggtttttcac cgcaacagat tgatctgggg agatatctgt tttttgatcc cttgctttca
      481 ggtaatcatt ctttgtcctg tgctagctgt caccacccaa atctgggatt tagtgatgcc
      541 agagcaaggt cgatcggtgt taatgcttta gatgtaggtc gagcgtcacc aacgctgtgg
      601 aatgtaggat tcctgacaac gtttttttgg gacgcgcgta cacactcatt agaggatcag
      661 atgatcggtc cactctttgc agagaaagaa atggccaata ctccagaaaa cttagtccaa
      721 gagatcaacg ctaaccaaat ataccgaaca ctctttgcca aggcttatcc cggtacagat
      781 aaagagttga ttacattgaa tcagatttat accagcattg ccgcttttga ggcatcgttg
      841 atcagcctta acagtcgcta cgaccaatac gctcacggtc atcatcaggc tttaacactc
      901 aatgaaatac aaggcttgaa tatttttcgt tcctttgttg ctcgttgcgc tgaatgccac
      961 acaccacctt tgtttactaa tcaacaagta gccgtacttg gcacacctga accacaaggc
     1021 agaccattgg atccgggtgc tggtaaaata tttgatagtc ttaagcttcg tggcggcttc
     1081 aaagtgccaa ctttgaggaa catcaaccaa actgcccctt atatgcactc tggtgctttc
     1141 actaatctaa gggatgccgt ggaattttat accaagggtc gtgggcatgc tgtcccagaa
     1201 ggcgaagaat tattattaca ttggcatatt tgggaaccag agctcactga taacgagatt
     1261 gatcttttgg tagacttttt acttacgcta acagatgaaa gcggtaagcc tcaggttcct
     1321 gaaagtgtac cttctgggtt accggtggta tcggtagaga ccatgcaact taatcaatcg
     1381 gcacatgcaa taggagaaaa ataatgaatc aatcacgaaa atgggctgtt tattttttta
     1441 tcgtagtggt ttttgccgcg gggtttctaa ttggcaagtt gaacgttgat ggatcgaaaa
     1501 cattaattgg tgatgtcgac aaatatcaaa cgtctataca ggcaaggaat gatagtgaaa
     1561 cacagtacgg ctcagttata agcattgaac gcgagccgtt taacggacag atccatacca
     1621 ttgaacaagg cgaatctatt caggatgcag ttaatgacgc acagcctggt gatgtaatca
     1681 gaattctacc tgggacctac aaggaaaccg tctatattga taaagataat attactctga
     1741 caggtgtaat cattaaggga gaatggccaa ctcttgaagg gaattatgag ttgaatgatg
     1801 cgatcttata ttccggcaac aatttcaccg tagaaaacct gaagataact cattataaag
     1861 gcaacggtat tatgggtcag gcgggtaaca attttattat tcgcaacaac ataattctag
     1921 actctggtgt atatggaata tttcccgaat ttggaaaaaa cggcatcatc gaatacaacg
     1981 tactgactgg cactgaagat gctgcaatct atgttgggat gtgcgacaac gtccatgttg
     2041 ctcataatga agtctttgct aatgttgcag gaatcgaaat cgaaaatact cgccatgcta
     2101 tcgttgaaaa caattacgct cacaacaata ccggtggtat tctagttttc attaccccgg
     2161 gactaccaat taagaccact tatgatgtca ttgttagaga taactatgta gttgataata
     2221 accatgaaaa ctttggtgca cctggttcca ttgttgctgg aatacccaag gggacaggca
     2281 tcctgattat ggcgggggat gatgtgaccc tacagaataa tgttattact ggtaacaata
     2341 atgctggcat cattattact gatcatggca atgccccaaa tctgacactg gatccagaag
     2401 tagagcctaa ctcagataga atagcgatcc ttgataacct aatgtatgac aacggtgctg
     2461 acccctcaga attagtgaag gcattgatgc ttaccaaaat gacaacgcgg ggaccggata
     2521 ttattcgtgt tggtgaaagc aaagacagtt gtattcttaa tcgtgaaaaa tatcttagct
     2581 ttggtataga cgaatttggt acttgtgctt ttaccagtac ctatggtact aaaacctatt
     2641 tgcttgaaaa gcctgtagtg atgcaggcaa tcaatgcaga agaacgaggc aaactaactt
     2701 actacagtgt ttgcactggt tgtcatgctt acaacgtaag aatggttgga ccgccaactc
     2761 aagagatcca ggcactctat tctgataatc ctcagggcat tgttgattac atcagcaatc
     2821 caactaggaa gcgagctgat tatcctgaaa tgccgccaca gaattaccta tccatggaac
     2881 ttaggatggc cgcggcacaa ttcatgcttg atgctgaaca atagaactat caacctatcg
     2941 agcagcagcc agcgctgctt tgaaatgaaa gcccattgcg attactacga ttactatgag
     3001 attgaacgtg acttcgaggt agccgtgaat cgcgggcaga tctacaatac cgaccagcat
     3061 catagccacc agaacgtgat aggagatacc aatatatccc agagtaaccg ctgcaatatt
     3121 aatgatgtaa actattgttt tgaaaatgag taaacaaata acacgtcgtg atttctgtaa
     3181 tggtatagct atcggcacag gaatcagtct cttgagcacc atggatttat ttgggcaaaa
     3241 cgcatttact attcaaaacc cagaacaacc cttcgcttat tatcctccaa cacttaccgg
     3301 aatgcgtggc agtcacaaag gttcttttga ggtggcgcat gctttggctt ggactggaga
     3361 aaagcccgct aaatatgaaa aacttgatga agagtatgat cttgtaattg ttggtgcagg
     3421 tataagtggt cttgcaactg cttggtttta tcaacaaaaa atgggccaag atgcacgcat
     3481 tttaatattg gataatcatg atgattttgg tggccatgct aaacgtaatg aatttaatca
     3541 tgatggaaaa ttgttattgg gtattggtgg ttctgttaat ctcgaaaatc caagagatta
     3601 tagtgaggaa gcaaaagggt tgctccatga cttaggtatt gatttagatg cgatgaggaa
     3661 taatataaat gatgatcatt atgcactggc aaatccagaa tcaaatcacg ctttagccct
     3721 acctggacca aatggtcatg taaccgttaa agctaattgg atattccttt atcaaggtga
     3781 aggggatatt gagaccgcta ttaaatcatt gccgttacca gtcattgagc aagaaaaatt
     3841 aatagaattt ctaagcggag aacgtgatta tcttgatgat ctctctcttc gggaaaaata
     3901 taattatgtt caaactgtaa gttatagtcg gtttctgtca gatcgggttg gtttagatga
     3961 agaaacctca tcaatattct atgcatggat gaagctcatt tattgtgttg atggaagaaa
     4021 tgttagcgtc ctggaagcac ttttagttgg tgcgcccggt atgcaaggaa tgggtagact
     4081 agctaaattc atacaatctt tgggttctct aagtattgat aataatgaat ctttgtattt
     4141 tccagatgga aatgcctcca taccaagatt gttggtaaag aaattgattc ctgcggtaac
     4201 atctagcgaa gcaaacttta ataatatttc tacgagtcat tttgattaca gcttgcttga
     4261 tcgtagtgac cattcaatcc gattaagact caacagtacg gtagtgagtg ttagacaaaa
     4321 taaagatgat ggagtagatg tagattatgt aaaggatggt aaggcattaa gagtcaccgg
     4381 caaacacagt gtcttagctt gttacaatga gatgattcct cacttgtgcc cagagttacc
     4441 agaatcacaa aaagacggtc tacgttatgg tgaaaaagca ccacttgttt gggccaatgt
     4501 ccttttagaa aatggcgatg ccttctcaga gcttggtgtt aacctggtca cttgtccaaa
     4561 agaccctttc gtcgttgtca ctactgcacc tccaacctca acaggcgaac atcaaccacc
     4621 gaaagaccct agtgatccga tggtggtttt tatgatgagt attccaagcg taaaatcaac
     4681 tggcaaagag actgcacgtg aattatttaa ggtaggtcgt caggttgttt attcaacttc
     4741 atttgcaact tatgagaaac aagttcgtga tcagcttcag gtgttattag gacctcatgg
     4801 ttttaatcat gaggtcgata ttaaggctat tacggtgaat cgttggccac atggttatgc
     4861 ttatgaatat tttgcattag atgatccatc atgggaagag ggtcaagcgc cacatgaaat
     4921 cggccgtact caatttggaa atatttctat tgctaattcg gactctcaag cgcttgccta
     4981 tcttgatgct gccatcgatg ctgcttggcg tgctatagaa gaacaaacag cgtaaaaaaa
     5041 gaatgctatg gaaaatgatt gattcggaaa gtaaaccaaa ggttagaaaa aaactcccaa
     5101 cccatgcttt gactaaaaag cacaagaata agaaaaaata taatcgaaaa gaaaaaggaa
     5161 gaaaagcaga taccaaaata cagggaataa ttgatgatca ataaattacg tctttccttt
     5221 ctagcttcgg tcattttttt tatttcctca atatttttta ttccactgca atcgagtgag
     5281 ataaaaactc aattaatact tcttggaacg ggaacaccca atgctgagcc tcaacgatca
     5341 gggccttcct tagctgttgt ggttggagac aattcttata ttgttgattt tggccctggt
     5401 atcgttagga gggcaacagc actttcgaaa caatgggagg gcagttttca agcactggat
     5461 ccagaaaact taaaaatagc tttcttaaca cacctacatt cagatcatac cggagggtat
     5521 cctgatctta tactaacccc ctgggtttta ggtagagaaa cacctttgga agtctatggt
     5581 cctaagggtt tatcgaagat gactgaattg attatagaag cttataagca agacatagat
     5641 taccgtctag aaggccttga accagccaat caatatggtt ggatggttaa tgtgtatgaa
     5701 attgaagagg gcattgttta tcgggacgaa ttggttgaag ttactgcttt taaagtcaac
     5761 catggatctt gggacaatgc ttttggttat aaattcgtta ctcccgataa aactatcgtt
     5821 atttctgggg atacgggacc tagcgggaat ctgattaact atagtaaaga ggctgatatt
     5881 ttagtacatg aggtttattc acaagctggc tttgagaaga aagacgaggt ttggaaaaaa
     5941 tatcattcaa cacatcatac ctcgacctat gaactgggtg agattgcaaa cataatcaaa
     6001 cctggcttac tggttctaac ccatgtactt ttttggggct caactgagaa agagatattg
     6061 gatgagattc atgaagtata taatggtcct gtttcttttg gatcagattt aatagtctat
     6121 tgactaacca gaggttgaaa tgacaccaag caataacaaa aagactgtct taattacagg
     6181 cgcatccgca gggataggca gagcttttgc cgaggtttgg gcagaggctg gattcaatct
     6241 tttgctgacc gctcgaaggg aggatcgact ccaggaaatc gcccatcaat tgcagtcaga
     6301 acaagacgtt caagtgaata ttataccgat ggatttagca gatcccgaag ctcccaagaa
     6361 gatccatcag cactgcttgg ataacaacat tcatatcgat gctctgatca ataatgcagg
     6421 ttatggaatg tctggagaat ttgaacaaat gaattggaac tcccatcaag attttttaca
     6481 agtcatggtc aatgttgtcg tgcaactgac ttacttattt cttccaggga tgattcaaaa
     6541 aaagtatgga agaattatta acctcgcctc aatggcaccg ttcatgccat cgccaagtat
     6601 ggcgggtttg tatagtcctg tgaaaatttt ccaaattaaa ttttctgaaa gcattcagat
     6661 tcaatatttt gataaaggga tttattgttc agctgtctgt cctgggttaa ccagatcaga
     6721 gtttcacgaa gcagctggaa tgcatgaagt ggtcaacaat tcacccaact ggctatggat
     6781 ggattctaga accgttgctc agcaaggttt tgatgctgta atgaaaggga agactttgat
     6841 tattaatgga acattgaata aagcttttgt gattctggca aaatttattc ctgacaaagt
     6901 aacaagatca gtggcaaaat atctatccaa aaggtcttat tgatcatgca taacggaatt
     6961 aaaacaactt ttttattaca gctcatggcg ggattaatct tggtgttgcc tgtttacggg
     7021 caacaagata gtggttgggt taacggcagt aatatatctg aatcaattaa tgagacatca
     7081 ccaattggat tagtagatat gccctcgggg acagctgaag attttgaaga cgatacattg
     7141 gtggattcta aatttcttga taaacaaaaa gatcagaata gagatttttt cattactggt
     7201 aaagcaagga tcatcgacgg tgacaccatc agcatcaaca aaactaaaat tagatttagt
     7261 ggtatcgatg ccccagaaag ttactattat ggccaaaccc aatattgcaa aagaagtaat
     7321 ggaaagatat gggcttgtgg aaaaaaagcc actcaaaaac ttaaagaatt gatcaatggg
     7381 caagaggttc aatgcactga cgaaggtaag gataagtatg atcgaacact cagcatttgt
     7441 tatgcaaatg gtatggattt acaggcagaa atggttcgat caggcatggc tatggcttat
     7501 ttgaagtatt caaatagata cgaaaatgaa atgattgaag ccatgacaaa cagagtaggt
     7561 atttggagcg gtgattttat cgatcctgat ctttggcgac aacagaatag aaaaaaatag
     7621 ttaataaata taatactcgc ttccagttct tattttttat caggagagtg gaatgcaatt
     7681 agtaggatag ggaaataaaa gaatagctgt aaaatgcacc aaagcgtgaa acttttaccc
     7741 tttcttttcg cagtcagacc tgtccaataa gacaatacta aattaataat cattaagctg
     7801 atcgcaagat ccataagtac tctttgttgt ttaatactac ccactcatta ctcttggcca
     7861 acgttggtgg atactgtgtt ccaattcagg ttctatcgta acaacataca atctttgttc
     7921 ttgtttctca acagctatta atccttggat ccattgccct ttgactaaat caaaccaatg
     7981 acttgtgccc tcgtgatctt tctccatgaa actgtgtacc ggtattttga ttgcctttgg
     8041 aaaccatctg tcccagatgc cagaatgaat tgaatccagc cttgcccatc cacccatggg
     8101 taattttcca gcttgtcctt ttcttcttcc ccataaaatc aaggatgttt cattgttttt
     8161 atttaaaaca ggtaattttg ctttagggtt tggaaaataa accctcatat gatcgccttt
     8221 gtgtgtatat gttgctccgc cacacatatt aaatagttcg cctatggcca tagggcttcc
     8281 acgcaggggc aatgacatct ggcatactgg atcgatgaat taggttactt ggagctatgg
     8341 tcaggtcgcc gtaacgttga ttaattgcat ccataacgct atttttttca tgagaagatg
     8401 accactgttt tttgttttca aggaaatcga gctgttgatt cgattgtttt ggatgtattg
     8461 ctgtcacctg aatttggttg attccttcac catgccattg cttatcaaga agctgttgac
     8521 ataaatccat aattttttga ccatcatttg ttggatattc ggtttgcatt ttcccaccaa
     8581 tccatccgtg ctgcgaaagg atgccaataa aaaatttaga agtttgtaga ttatggcgcc
     8641 taaggcggct gccaactttt tcgctcatgt gttgcagata caccaggatt atttttttgt
     8701 ttctggtatt gggtggcatg actttgccat gtccaattga tttgggtccg gcaacattag
     8761 tcttaataga ttcaggatct ttgccttgag ccataagcca aatacgacgc cctggattgc
     8821 caaaatgctg acctaggata ctgatgggaa tatttttcat atcaccacaa gtatagaccc
     8881 cgtaactatt tagaaatctg cctatacctt ttgctatacc acacaaatca gtcacaggag
     8941 cattttttag catgttctct gcttcccctg gggggataat ggttaagcca tcaggtttct
     9001 cttgtttggc ggcgtatttt gctgtcgttt tgtcaccact aataccgata gagcaagata
     9061 aatctgatag ttcaaaaatt aatttcttaa tgctcttggc cactttttct ggtgactcaa
     9121 tgattttttg gcaatgtgtc atatcaagaa aggcttcatc gacggaatac acttcaatat
     9181 ctggggtgat agtttttaga ccatgcataa ttttggtaga tatttgtgca taacgttttg
     9241 gtctagaagg tctttgaatt aattccggac atagttgttt tgcttgtttg agacgcatgc
     9301 ctgttctgat accccaatac ctagcctcgt aggagcaagt gatgatgcag gttccttgat
     9361 ttccattggt aacggcaacg gggcgtccaa ataattctgg ctggtcaagt tgttcgattg
     9421 acgcaaagaa tgaattcatg tcaaccaata cgatcattcg aggccaaggc aatgaatttt
     9481 tattactggt agcttctaag ttgcccaacg acgactccct gaatttggat acgctgtgct
     9541 ggatataccc taggttctat aaatttgttt tctggcatca ataaaatcat tccatcaggc
     9601 agttttttaa gtcgttttaa agttgcctca agaccatcaa tgagtgcaac tacaatgtct
     9661 ccgttatttg cggtttgttg atgtttaacg attaccgtgt ctccatctaa aatgcctgct
     9721 tcaatcattg aattcccagt gacctgaaga gcaaaacgat ctgcattcat taagaaatca
     9781 gctaaattaa gttcatctcg tccttcaatg gcttcaatag gttggccggc tgcaatacgc
     9841 cctaataatg gaataatagg tgctgctaat gcattctcat tggcctctag atttaaacga
     9901 ataccacgtt tacggtttgg caatatggtg attaaatcag atgattgcag agctctcaag
     9961 tagcgatgta tgacacctct tgatttgatt ccagtaccct gtgcaatttc ctgtagggta
    10021 ggggcataac cactctttac aaagtatttt tgaataaatt tcaatgttct tgtttcactc
    10081 ggagttaaca tgatttctgt cccatgtatg attcattttt atttaaacaa tgatagagaa
    10141 caaatagaga acattcaatg aaatttctga aaataatttt tgatatagaa tttaataatt
    10201 ctgttagctt gaaatctata gcgatacaat aataattaat atatttgagg acaaaatgtt
    10261 acctttggaa ttaatcatac gcgcgctcag tattattttt ggtttgatca tatttattct
    10321 tgccataata tttgggtggt ttatcttttc tattttggta ggggttttgt tttttatttg
    10381 gataatattt tatgtccgga gtttgtggaa tcgacgcagg gaaaaaacag gtgccaatga
    10441 tgcaataatt gatgcagaat acacagtcat agatgtagag gattcagatg attgacagga
    10501 tcgaggaata taaaaataaa agaaggaaaa gacgcaccag gcgaataatt tttgttttgc
    10561 ttttcgttgc ttttgcaacg atgctctctt ggttttttga atcgcaagct accacgacag
    10621 tgatattaac aacacatgca gatgttgatt caaact
//
LOCUS       DASCTH010000031         1733 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000052114, whole genome shotgun sequence.
ACCESSION   DASCTH010000031 DASCTH010000000
VERSION     DASCTH010000031.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000052114"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="QGH35_00705"
     CDS             complement(<1..1709)
                     /locus_tag="QGH35_00705"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HJL81084.1"
                     /translation="MDSLAQLISPQLSAHSDKILLNKKLFQRIKSIYEERHSSWSHSF
                     QKKLSPEQRRLVEETHKHFVRAGVQLNEASMKSLTEINQKIATLSVQFDQNLLKETNE
                     GFSLIIKDENQLSGLPEDVIDQASKLADSEGNESHWKFKPNRASMYPFLTYSTQRDLR
                     EKLYNAYIKRGDNDNERDNKNIAIKMAALRIDKANLLGYDNHADFVLEDTMAKNTKRV
                     NDLLEQVWKPGLSRAEKEVQEMQELINAEGNDFEFAAWDWWHYSEKIRQEKYDFTEAE
                     VKPYFSERKVLQGAFDVANKLFQISFTERFDFPKYRENIRSFEVKNEQGDTIGIFYTD
                     YTIRPNKGGGAWMNTFRSQSTFDGVQIPIVVNVCNFPPENKDGFALLSFEQASTLFHE
                     FGHALHGLLSKATYPSLSGTRVARDYVEFPSQLMENWAIEPSVIKSYARHYQTNEPIP
                     LELLDKISKASLFNQGFATTEYVAASYLDMAWHTLEEPIEDANLFETKILQDLDLIPE
                     ITSRYRSTYFAHIFAGGYSSGYYSYLWTEVLDADAFELFKEKGLFDPETANKLKTLIY
                     SSG"
BASE COUNT          457 a          346 c          331 g          599 t
ORIGIN      
        1 ttccctgatg aataaataag agtttttaat ttatttgcag tctctggatc aaacaatcct
       61 ttttctttga ataactcaaa tgcatctgca tcgagtactt cagtccaaag ataactgtaa
      121 tagcctgatg agtatcctcc tgcaaaaata tgagcaaaat aggtacttct atagcggctg
      181 gttatttcag gaatgagatc taaatcttga agaatcttag tttcaaataa attagcgtcc
      241 tcaatgggtt cttccagggt atgccaggcc atatcaaggt aggaagctgc cacgtattca
      301 gtggtagcaa acccctgatt aaataaagat gccttagaaa ttttatcaag caattccaga
      361 ggaatcggtt cattcgtctg gtagtgtctt gcgtaagact taatgacgct tggttctatg
      421 gcccagtttt ccatgagttg tgaggggaat tccacataat ctctcgctac tcttgtgccc
      481 gagagactcg gataggtcgc cttggacaat aatccatgga gcgcgtgtcc gaactcatgg
      541 aacaatgttg atgcttgttc aaaggaaagc aaggcaaaac cgtctttgtt ctcaggaggg
      601 aaattgcaaa cattaaccac tatcggtatt tggacgccgt caaatgtact ctgactccta
      661 aaagtattca tccaggctcc ccccccttta ttgggtctga tggtgtaatc agtatagaaa
      721 attccgatgg tatcaccctg ttcatttttg acttcaaatg aacggatgtt ctctctgtat
      781 tttgggaaat caaatctttc agtaaagctt atttgaaaaa gtttattggc aacatcaaaa
      841 gctccttgaa ggacttttcg ttcgctaaaa tacggtttga cttcagcttc ggtaaaatca
      901 tacttttctt gccttatttt ttctgaataa tgccaccaat cccaagctgc aaattcaaag
      961 tcatttcctt cggcattgat caattcctgc atttcttgaa cttctttctc tgctctggag
     1021 agtcctggtt tccagacttg ctcaagcaga tcattaactc tctttgtatt ttttgccatg
     1081 gtatcttcaa gaacaaaatc ggcatggttg tcgtatccaa gtagattggc tttatcaatg
     1141 cgaagggctg ccatttttat ggcgatgttt ttgttatctc gttcgttgtc gttatcgcct
     1201 ctcttaatgt aagcattgta gagtttttct cttaaatctc tctgtgtgga gtaggtaaga
     1261 aatggataca tactggctct gtttggttta aatttccaat gactctcatt accttctgag
     1321 tcagctaatt ttgatgcttg atcaattaca tcttctggca atccgctcaa ttggttttca
     1381 tccttaatga tcaaactgaa tccttcgttt gtttctttca acaagttctg atcaaactgc
     1441 accgagagag tagctatctt ttgattgatt tcagtcaagc ttttcataga tgcttcattc
     1501 aactgcactc cagcacgaac aaaatgctta tgagtttctt ctactaatct cctttgctca
     1561 ggacttaact ttttttggaa tgaatgagac catgaactgt gtctttcttc ataaatgctt
     1621 tttattcttt gaaagagttt cttgttcagt aatattttat cgctgtgggc ggagagttga
     1681 gggctgatga gttgagcgag actatccatt tcatcatttg tatttgatcc aag
//
LOCUS       DASCTH010000032         5251 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000053395, whole genome shotgun sequence.
ACCESSION   DASCTH010000032 DASCTH010000000
VERSION     DASCTH010000032.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000053395"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2168)
                     /gene="alaS"
                     /locus_tag="QGH35_00710"
     CDS             complement(<1..2168)
                     /gene="alaS"
                     /locus_tag="QGH35_00710"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HJL81085.1"
                     /translation="MKTNEIRELFLNFFESREHEKVSGSSLIPSNDQTLLFTNAGMVQ
                     FKDVFLGNEQRQSQRAVSVQRCLRAGGKHNDLDNVGYTDRHHTFFEMLGNFSFGDYFK
                     EEAIHFAWEFITEELNIDPDRLWVSVFKDDDEAAAIWLDQIKVNPDRLVRMGEASNFW
                     SMGQTGPCGPCTEIFYDHGPHIEGGPPGSESEEGDRFVEIWNLVFMQFERAEDGQLTQ
                     LPKPSVDTGMGLERIAAVMQGVQSNYEIDLFKNIIKAVSSLIGDAGSNNHYRVISDHI
                     RAAAFLILDGVSPGREGRGYVLRRIIRRALRHGYELGVEEPFFHEVIDPLVKEMGEAY
                     PDLAQKSEYIKRIILTEEERFSATLAQGMRLLETEIDSLSGDILSGEFVFKLYDTYGF
                     PVDLTSDIARVKQLKIDQKGFDKAMDRQKEMARTASQFSRQQVKEVDHGLETSFLGYD
                     QHENDTKVTGIIKDQLINQIAKGETGQVILEETAFYAESGGQVGDVGTIQSKTGAFQV
                     LDTQSSGDALVHLGTVTNGTMALNDKVKATINPIRRQAITLNHTSAHLLHSALINVLG
                     DHVAQRGSAVGDKRLRLDFSHDQALTTEELRQIEELVNKKIEKNIDVETSIMKHDEAL
                     ESGALALFGEKYGEEVRVVNVELFSKELCGGTHVKNTGEIGFFKIISESSIASGTRRI
                     EAITGDEALKWAEETEYLVEKLADMLQTGRDGIEKKVQTLV"
     gene            complement(2173..2652)
                     /locus_tag="QGH35_00715"
     CDS             complement(2173..2652)
                     /locus_tag="QGH35_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HJL81086.1"
                     /translation="MTEDHSENVSIRKKAMDLLARREHSFFELKTKLLQKNFNEDAIN
                     QELHRLVDDHLLDDSRFTESFLTFSLRKGKGPLRIESELQQRGVSGDVIQRFLLEVDD
                     HEWIQIATDVLKKKLGTAKQVDYDKKLKLMRFLSNRGFTNLQVIKAISPFVANVDDE"
     gene            complement(2673..3704)
                     /gene="recA"
                     /locus_tag="QGH35_00720"
     CDS             complement(2673..3704)
                     /gene="recA"
                     /locus_tag="QGH35_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HJL81087.1"
                     /translation="MNPKNRNKALDSVVSQIEKQFGKGSLMKLGEDHVDLAIESISTG
                     SIGLDHALGIGGLPRGRVVEIYGPESSGKTTLTLQVVAECQKTGGTAAFIDAEHALDP
                     VYAEKIGVNTKELLLSQPDTGEQALEITDMLVNSGAIDVIVIDSVAALTPRAEIEGDM
                     GASHVGLQARLMSQALRKLTANIQKSNTMIIFVNQIRMKIGVMFGNPETTTGGLALKF
                     YSSVRLDIRRIGAIKKGDEIVGNQTRVKVVKNKVSPPFKIAEFEIIYGHGISREGEII
                     DIGVEHELIEKSGAWYSYQTERIGQGKENVRQFLLDNKAISEDLNTKIRNILLPDRNS
                     ESDEESKEE"
     gene            complement(3876..4880)
                     /locus_tag="QGH35_00725"
     CDS             complement(3876..4880)
                     /locus_tag="QGH35_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HJL81088.1"
                     /translation="MTLTQLKYLIAVVDNRLNISLAAENLNTSQPGISKQLKLLEEEL
                     LATIFVRKGKKLTDLTPFGDEVVFYARKILQDVSNIGLLGQQLSSVDEGVLTIATTHS
                     QARYVLPDVIKTFKLDYPKVKLELKLGSANQIQEMVQTSEVDIAIATDLLELQRELVL
                     LPAYNWYHAIIAPLDHPITKDFKRLTINRLAKEPLVTYAFSVEPDTSFSETFENIGLK
                     PNIVFSARDADIIKTYVNMGMGVGVIAGMAYHCEDHEVFAITSGENLFPQCTTWFGFR
                     RGMLLRNYLVNFINLFTPALSPKLILKAAETSSQSELNKLMKDIDLPSKGGCDEININ
                     "
     gene            complement(4968..>5251)
                     /locus_tag="QGH35_00730"
     CDS             complement(4968..>5251)
                     /locus_tag="QGH35_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81089.1"
                     /translation="TVMLAENPFDLDVYFDSTGSVSTNLEYQGSWWVKGDTGEQLFCY
                     KITSSQREPSILSECFPLILMNNPRIGARWPSKFDQNIMYMAVVVEGRP"
BASE COUNT         1506 a         1172 c          863 g         1710 t
ORIGIN      
        1 actaaagttt gaactttttt ctctattcca tcccgtccgg tttgcaacat gtcagcaagt
       61 ttctcaacca agtattctgt ttcttcagcc catttcaatg cttcatctcc tgttatagct
      121 tcaattctcc tagttcctga ggcaatactc gactctgaaa taatcttaaa aaatccaatt
      181 tctccagtgt ttttaacatg cgttccacca caaagttctt tggagaataa ttcaacattg
      241 accactctaa cttcttcacc atacttttcc ccaaacaaag ccaaagcacc tgattctaag
      301 gcttcatcat gtttcataat acttgtttca acatcaatat tcttctctat ttttttattc
      361 accaattctt caatctgtct taattcctct gttgttaatg cctgatcgtg agagaaatct
      421 aaacgaagtc ttttgtctcc aaccgcagaa ccccgttgag caacatgatc acctaagaca
      481 ttgattaagg cagaatgcaa taagtgagca ctggtatgat ttaaagtaat tgcctggcgt
      541 ctgatcggat taattgttgc tttgacttta tcgtttaatg ccatagttcc attagtgact
      601 gtgcccaaat gaaccaaagc atctcctgaa gattgggtat ctaatacttg aaatgcaccc
      661 gtcttggatt gaatggtccc aacatcacca acctgaccgc cgctctcagc atagaaggca
      721 gtttcttcaa ggataacctg accggtttcc cctttggcta tttggttgat caactgatct
      781 ttaataatgc cagttacctt tgtgtcattt tcatgttgat cataccctag gaaactcgtt
      841 tccagaccat gatcgacttc cttcacttgc tgtcgagaaa attggcttgc ggttctagcc
      901 atttccttct gtctatccat tgctttatca aagccttttt gatctatttt aagttgcttc
      961 actctggcaa tatcagaagt cagatctaca ggaaatccat aagtgtcata aagtttaaaa
     1021 acaaattctc cagagagaat atccccggaa aggctatcta tctctgtctc cagcaacctc
     1081 ataccctgcg ccaaggtagc tgaaaaacgc tcctcctctg tgaggataat tcgttttatg
     1141 tattcacttt tctgagccag gtcaggataa gcttcaccca tttctttgac taaaggatct
     1201 atgacctcat gaaaaaatgg ttcctcgact cccagttcat atccatggcg tagagccctt
     1261 ctgataatcc ttctcaaaac atagcctcga ccttctctgc caggactcac gccatccaat
     1321 attaggaaag ctgctgcccg aatatgatct gaaatgactc gatagtgatt attgctgcca
     1381 gcatcaccaa tgagggagga gactgcttta ataatatttt taaaaagatc aatctcgtag
     1441 ttgctttgta ccccttgcat cacggctgca atcctttcaa gacccatgcc tgtatccact
     1501 gatggtttcg gcagctgagt taactggcca tcttctgctc gttcaaattg cataaaaaca
     1561 agattccata tttcaacaaa tcgatctcct tcttcggatt cagaacctgg tggcccaccc
     1621 tctatatgag gtccatgatc ataaaaaatt tcggtacacg gaccgcaagg tcccgtttgc
     1681 cccatggacc aaaaattaga ggcttctccc attcgcacca accgatctgg attgacttta
     1741 atttgatcca accaaatagc agcagcctca tcatcatcct tgaaaacact aacccataaa
     1801 cggtctgggt caatgttgag ttcttctgta ataaactccc atgcaaaatg aatagcttct
     1861 tctttgaagt aatctccaaa actaaaattg cctaacattt cgaaaaacgt atggtgccta
     1921 tctgtatagc cgacgttatc caaatcatta tgcttacctc cagctctgag acatctttga
     1981 accgagaccg ctcgttgtga ttgcctttgc tcgtttccta aaaaaacatc tttaaattga
     2041 accattccag cattggtaaa cagcaatgtt tgatcatttg aagggatcaa tgatgaacct
     2101 gaaacttttt catgttccct tgattcaaaa aaatttaaaa acaattctct gatttcattc
     2161 gttttcattg ctttattcat catcaacgtt ggctacaaag gggctgattg ctttgattac
     2221 ctgcaaattt gtaaatcctc tattacttaa aaaacgcatc aattttaact ttttatcata
     2281 atcaacttgt ttggctgtac ccagtttttt cttcaataca tctgtggcga tttgaatcca
     2341 ttcatgatca tcaacttcaa gcagaaagcg ttgaatgaca tcaccactga cacctctttg
     2401 ctgaagttct gattctatcc ttaatggacc tttgcctttt ctcaaagaga aagtgagaaa
     2461 ggattctgta aatctcgagt catctaaaag atggtcatct accagtcgat gaagctcctg
     2521 attaatcgca tcttcgttaa aatttttttg taataatttt gtttttaatt caaaaaatga
     2581 atgttccctt ctagccagta gatccatggc ttttttacga attgatacgt tttctgagtg
     2641 atcctcagtc aaaatgaaat gattctatga gtttactctt ctttagattc ttcatcagac
     2701 tctgaattcc gatcgggtag aagaatgttc ctaattttag tgtttaagtc ctccgagatc
     2761 gctttattgt ccaataaaaa ttgcctcaca ttctcttttc cctggccaat tctctcagtt
     2821 tgataactat accatgcacc cgatttttct attagttcat gttccacacc gatatcaata
     2881 atctcccctt ccctggaaat tccatgaccg taaataattt caaactctgc gatcttaaac
     2941 ggaggtgaaa ccttattttt aactactttc acccgagttt gattacccac aatttcgtcg
     3001 cctttcttaa tggcgccgat ccttctaata tctaaccgga ctgatgagta gaatttgagt
     3061 gccagcccac ctgttgtggt ttcaggattg ccaaacataa caccgatctt catgcgaatt
     3121 tgattcacaa atatgatcat cgtattggac ttctgtatat ttgcagtcag ctttctgagt
     3181 gcctgtgaca tcaatcttgc ctgtagacca acgtgactag cacccatatc tccttcaatt
     3241 tctgctcgag gagtcaaagc ggcaaccgag tcaatgacaa ttacatctat tgctcctgag
     3301 ttgaccaaca tatcggtaat ttcaagtgct tgttcacccg tgtcaggctg agaaagaaga
     3361 agctcttttg tattgacacc tattttttcg gcatacaccg gatccaaggc atgttctgca
     3421 tcgatgaatg cagcagtacc acctgttttc tgacactctg ctacaacttg aagagttaaa
     3481 gttgttttac ctgaagactc aggtccatag atctcaacaa cccgtcctct gggaagtcca
     3541 cctataccta atgcatgatc aagaccgatt gaaccagttg atattgattc aatagccagg
     3601 tccacatggt cttcaccgag cttcattaaa gagcctttgc caaattgttt ctcaatttga
     3661 ctaactactg agtccagggc tttatttcga ttttttggat tcatgttttg ttaccaaaca
     3721 tttatggttg tattgcctta aatgagtatt ctagtaaaaa ttatatcctt ttagataatt
     3781 taattcttaa ttgatttgac tctttctgat gaaccatcag agttgtcaga aaatacaaat
     3841 ccagagaggt cagttaatct ttgaatgatg tcattttaat ttatattgat ctcatcgcag
     3901 ccgcctttac tcggcagatc aatatccttc ataagcttat tgagctcgga ttgtgagctg
     3961 gtttctgctg cttttaaaat gagtttaggt gaaagagctg gggtaaataa attaataaaa
     4021 ttaaccaaat aatttctcaa taacatacct cttctaaaac caaaccaagt agtgcactgt
     4081 gggaaaagat tttcaccact ggttatagca aacacttcat gatcctcgca atggtatgcc
     4141 atgccggcta ttacacctac tcccataccc atattgacgt acgttttgat aatatctgca
     4201 tctcttgccg agaacacaat atttggtttc aatccgatat tctcaaaagt ttcactaaac
     4261 gatgtgtccg gctccacgct aaatgcatat gtcaccagtg gttcttttgc caaacgatta
     4321 atagttaaac gtttaaaatc ttttgttatg ggatgatcca gtggagcaat gatggcatgg
     4381 taccaattgt aagctggtaa taatacaagt tctctttgta actccaacaa atccgtagcg
     4441 atagcaatat ccacttcact agtctggacc atttcttgaa tttggttggc agaaccaagc
     4501 tttaattcta atttaacctt tgggtaatca agtttgaagg ttttgataac atcgggtaga
     4561 acatagcgtg cttgtgaatg ggtagtggca atagtcaaca ccccttcatc aactgatgac
     4621 aattgttgac caagcaatcc aatattactg acatcttgta gaatttttct tgcataaaaa
     4681 acaacttcat caccaaaggg agtaagatca gtcagctttt tacctttgcg cacaaaaatg
     4741 gttgccaata actcttcttc aagcagtttg agttgtttgc ttattccagg ttgactggta
     4801 ttcaaatttt ctgcagccag actgatattc agcctattat cgacaactgc aatcaagtat
     4861 ttcaattgag tcagggtcat gattgatcca tgtaaaagaa attatttata ataatttatt
     4921 atataaatta ttttaatata agtaatggta ataaatagga tctatggtta cggtctgcct
     4981 tcaacaacca cggccatata cataatattc tgatcaaatt tagagggcca tctggcacca
     5041 attctcggat tattcatcaa tatcaaaggg aaacattcgc tcaatatact aggctcccgt
     5101 tgactggatg tgattttgta acaaaataac tgctcaccag tatcgccttt cacccaccag
     5161 ctaccttgat attctaaatt tgtactgaca ctacccgttg aatcaaaata aacatccaga
     5221 tcaaaagggt tctctgccaa catgactgta t
//
LOCUS       DASCTH010000033         9672 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000053544, whole genome shotgun sequence.
ACCESSION   DASCTH010000033 DASCTH010000000
VERSION     DASCTH010000033.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000053544"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            315..1400
                     /locus_tag="QGH35_00735"
     CDS             315..1400
                     /locus_tag="QGH35_00735"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HJL81090.1"
                     /translation="MKILRTPDARFKNLKEYPFEPHYTTIKTHDDSDLRIHHLDEGPK
                     DGPILLAMHGQPVWSYLYARMIPFLTKAGIRVIAPDLPGYGKSDKPALREDYSYQRQV
                     DWMSQWLKENDFSDLTFFGQDWGGLIGLRMIAQDPDRFSKVSMGNTGLPYNPDTPQEV
                     IDEVKAFRQSDLKLTPISMAKEVRQMDGRSLSKESDVKFHPALKFMYWQKFCWETENL
                     PIGFMNSNMMEKRSKYSLMTQYFFHRVGFEKLSPCTSDLVKAYEAPFPDPSYKMGPRS
                     MPSQVPIIPDASLEAQREAREFFKTTTKPFLSVFAGDDPITNGIEKDVIEMAPNAKSA
                     PHIGGGHFYQWTKAKKLSDILISFIKE"
     gene            complement(1431..3161)
                     /locus_tag="QGH35_00740"
     CDS             complement(1431..3161)
                     /locus_tag="QGH35_00740"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81091.1"
                     /translation="MYKIIFIALSLMTLNACELSDKAPRADVVFTGGAVYRVTDTDPW
                     ATAVAITEKRITYVGDDVGAASSIGPNTRVVELDGKMLMPGFQDAHVHPVDSGMTFNQ
                     CAVFDLPQLQDLLDAIRACVDERPNAEWIVGAGWYVSTFAPTGRPPKELLDAIAPDTP
                     ITLLSNAAHTVWANSAAIKRAGITAETEDPEGGRIDRNPLTGEPSGSFQESAMPLIQS
                     FEPAITLKERIDGLAYAQRLFHSFGITGVHDSYVEVSRETAYTNMEAYTAFADKGKLK
                     MHVVAAMLYDPTLPLKPQIELFKTLRKMADRNHVKATAVKILVDGVATSYSAAMLKPY
                     ADRKDEGITGTPLIPRADLIDLVSRLDALGFQIHFHSIGDAATHYSLDALETARIQNG
                     PRDSRHHLSHIMVWDPADYDRMSDLDAIANFQALWASKDQHYDEITYIRMGKERSKYI
                     YPINSLHKRGVKLAFGSDWYVTSANPLLAIEVAVTRLDPDGYTTEPLLPVEAIDLQTA
                     IRAATLGSAYGNFLNEETGSIEVGKLADLIVIDTNLFDIPVSEISEAQVMLTLFGGEV
                     VFGSLDGVDN"
     gene            complement(3204..5582)
                     /locus_tag="QGH35_00745"
     CDS             complement(3204..5582)
                     /locus_tag="QGH35_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006005105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HJL81092.1"
                     /translation="MPSVSMSTVPIVQPGAPGNATREIDAETAVAIANSSYTVADAHF
                     MQDMIIHHHQAMVMSKFAAPSTNNQAILDLAGRIDVSQADEISFMQSWLQEREEQVPD
                     LVEDHSEHTHHNMMGMATPEQMTQLSESKSTDFDRLYLQLMITHHDGAIKMVEELREQ
                     PGSTYDPLLNEFASNVTNDQAVEIERMNALLIGLSSDPRAGLKAGLYDAGEAIMNMQL
                     LISQSKPLGFYDPANPEERGVDEPKDKQAGDEDKEQTIEKAAEDRRYPMLSFSNTDMA
                     FRDNLLVAGSYHGFNIYRIDEEGLPKLITSVVCPGGQGDVSIVGDLLIMSVEQTRGRI
                     DCGLEGVTVDASLDRFRGLRIFDISDLSRPMQVGGVQTCRGSHTHSVVAGPTPDGKIL
                     VYNSGTGSVREEEELDQCVKDIPGDDRTALFRIDVIEIPVDDPSQSSIIDSPAVFADP
                     ETGVLAGLWRGGDHGDDSQETEITDQCHDITVFPSANIAAGACSGNGILFNISDPRKP
                     ERIDVVTDTGFAYWHSATFNNEGTKVLFTDEWGGGGRARCRAWDPLNWGADAIYDIVD
                     EKLEFRSHYKMPAPQLETENCVAHNGSIVPVPGRDIFVQAWYQGGISVIDFTDSSKPL
                     EIAYFDRGPILEDELITGGYWSVYYYEGTIYGTEITRGLDALKLIPSEYLSENEIAAA
                     AMAYPIIGPRRVFNPQQQVPMMWPATPVVARAYIDQLLRDNAIAEDAAERIKDMLDQV
                     TQAMDNGGNNRLARQINSYRLSAKGSNVDALTRHRLEKLHAILKRIAAGLRS"
     gene            5808..6569
                     /locus_tag="QGH35_00750"
     CDS             5808..6569
                     /locus_tag="QGH35_00750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81093.1"
                     /translation="MFEFKAKKVLVTGASRGIGKAIAQGFAMKGAQVSLVYRKEKELA
                     DDLRNSLPGENHICVQSDVSVSEEAQYAVSTTLDVFGSLDIVINNAGMYIDHPIADVN
                     FDDWQDAWQSTINTNLIGPANICFHAAKHMIKKGSGKIINVSSRGANRGEPVAPAYGA
                     SKSGLNSMSQSLAVALAPYNIFVGVVAPGWVDTDMTSEFLSGEEGDAIRKQSPLGRVG
                     TSEEIAQAVLMLSMDGMEYATGTIIDINGASYLRS"
     gene            6582..8654
                     /locus_tag="QGH35_00755"
     CDS             6582..8654
                     /locus_tag="QGH35_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HJL81094.1"
                     /translation="MKELILITGLLLIGGYFSFTPNASFANHHNKIEITETWISMSDG
                     IRLAADIYWPAGADRNDRFPILLEYLPYRKDESRARSYSLYSYFLEHGYLVVRVDMRG
                     TGNSEGMVIPYEYSDIELDDGEEVIDWLSQQEWSTGSVGMFGISWGGFNSIQMAMRNP
                     PALKAFVALMATEFLYQEDVHYMDGIMHTDSWMMGNDLYNALPGAPDFKMDEEWLRNR
                     FNVKPSVYKYMRQQRDGEFWDRASARGKYDLIKVPGYHIGGWYDGYRNSLPRMLENVQ
                     APVKAMIGPWDHDFPHNAALQPQVEWRAEAVRWFDQWMKGIDTGILEEPDFVVYIRDY
                     HPPDDSLESIPGFWRWEEGWPINRIETQTWYAQANHKLSNISGVNDKHTLENIPSIGL
                     EGGGPTMWWGSIPSDQRPMDKNSLVYDSEVLVEPIEILGRPLAKLHVSANAKRANWVV
                     RISDIAPDGQITQVAGAAFNGTHRNSSRDPEDIIQGEKFPLNIQLHFTSWIFNEGHKI
                     RVAISNAMWPMLWPSPLPVKTLLEIGGEKGAYIELPIMPSGKIKTPEFEQLSIGPKLK
                     GYEVLDAGNVTGYAAIKSIQHDPDTGEKFGLATNTGATQYPWGIERFEEEIEHRTSDE
                     NPANTSVVGRYKITEELQDRTLDFEQNVEFKSDEENFYLIFHRWVSVNGELYKEKVWQ
                     EVIPRDFQ"
     gene            8770..9660
                     /locus_tag="QGH35_00760"
     CDS             8770..9660
                     /locus_tag="QGH35_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014615.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HJL81095.1"
                     /translation="MKYLLDSGMGDELKQRGFEVPDWKTSIWSVSALIKSPNGVVEIH
                     KDNIKAGCNVITTNNYYVTPNILKREGIESEFESLTRLAVDLAEQSRQGFPEVLIAGA
                     FPPIETSFRPDLTPSNEVLDDYYSNLGSIIQKKVDLILCETMGSIREGISAARNALEF
                     SDRVWLSWTNRGISGDILPSGEQLKDAVLAANQLDIECQLVNCCATDLATKNIEILKK
                     ATNFGIYANSRKLKEQKSIQDLGETQDIDVNHYANTVLISSEEYAREAKIWIDQGAYV
                     VGGCCTTRPEHIKKIADQLK"
BASE COUNT         2859 a         1952 c         2160 g         2701 t
ORIGIN      
        1 ggctcccaag aaaaacagac gaaatccgat acttaaaata ggtttcatat attatttatc
       61 cgttgatgtt atgtagattt ataaactaga aatacctttt ttaaattgat ataaatcata
      121 aatctaaaaa aaatcatcta tttcttaaat ttcattaaag ttagataatg aaggaaaatg
      181 aacttagaaa ggagtgggtt ccaattttta accatacgtt ccacaacaaa acaattaatg
      241 gaatgaaaat cgatagagtc acatcattct tatcgagtaa aggttgctct gagctaatta
      301 cataaaggta aacaatgaaa atattaagaa ctccagatgc aagatttaaa aatttaaagg
      361 aatatccttt tgaacctcat tacacaacca tcaaaactca tgatgactca gatttacgta
      421 ttcaccatct tgatgaagga cctaaagatg gaccaatctt attggctatg catggtcaac
      481 cagtttggag ttacctatat gcaaggatga ttccatttct aactaaagct ggtattagag
      541 ttattgctcc tgatttacct gggtatggaa aatcagataa accagcattg agagaggatt
      601 acagctatca aagacaggtt gattggatga gtcaatggct taaggagaat gatttctctg
      661 atttaacttt ttttggtcaa gattggggtg gattgattgg tttaaggatg attgcacagg
      721 atccagatag attttctaag gtttcaatgg gtaacactgg actgccttat aatcccgata
      781 ctcctcaaga ggttattgat gaggtgaaag cttttaggca aagtgatcta aaacttactc
      841 ctataagtat ggccaaagaa gttcgtcaaa tggatggaag aagtttaagc aaagagagtg
      901 atgttaagtt tcatccagcc ttaaagttca tgtattggca gaaattctgt tgggaaactg
      961 aaaatttacc tattggattt atgaattcta atatgatgga aaaaagatct aagtactctt
     1021 taatgacgca gtactttttc catagagtgg gttttgaaaa actatctccc tgtacttctg
     1081 atcttgtgaa ggcttatgaa gcaccatttc cagatccatc ttataaaatg ggtccaagat
     1141 ctatgcctag ccaggttcca atcattccag atgcatctct agaggcccaa agagaggcaa
     1201 gagaattttt taaaaccaca accaaaccat ttttatccgt gtttgctggt gatgatccaa
     1261 tcactaatgg aattgaaaaa gatgtaattg agatggctcc taatgcaaag agtgcacctc
     1321 atattggtgg tgggcatttc taccaatgga ccaaagccaa aaagctttct gatattctta
     1381 tatcttttat taaggagtaa taaggtgaaa aagagttctt tagtcagact ttaattgtcc
     1441 acaccatcca gacttccaaa tacaacttcc ccaccaaaca gggtcagcat tacttgagcc
     1501 tctgagatct cactaactgg gatgtcaaat aagttggtat caatcacgat caggtcagct
     1561 agctttccaa cttcgatcga gcctgtttct tcatttagaa aattaccata agcactacca
     1621 agtgtcgctg ctctaatagc cgtttgtaag tctattgcct caacaggtaa gagaggttca
     1681 gttgtataac catctggatc aagcctagta acagcaacct cgatagctaa taatggattg
     1741 gcggatgtta cataccagtc actgccgaag gccagtttta ctcctcgttt gtgcagtgag
     1801 ttaatgggat agatgtactt agatcgctcc tttcccatac ggatataagt aatctcgtca
     1861 taatgctgat ctttggacgc ccagagtgcc tgaaaattag caatagcatc caagtctgac
     1921 atccgatcgt aatctgctgg gtcccatacc atgatgtgtg aaagatgatg gcgactatcc
     1981 cgcggaccat tttggatgcg tgcagtttca agagcatcta acgaatagtg tgtagcggca
     2041 tcccctatgg aatgaaaatg tatttgaaaa cccaaggcat cgagacggct tacaagatct
     2101 atcaaatcag ctcgaggaat taagggtgtt ccggtaatac cttcatcctt ccgatcagca
     2161 taaggtttca gcattgctgc agaataagaa gtagcaacac cgtccactag gatttttaca
     2221 gccgttgcct tgacatgatt ccgatcagcc atttttctta gggttttgaa taattcaatc
     2281 tgtggtttaa gtggtagtgt cggatcatag agcatggctg caaccacatg catcttgagt
     2341 ttacctttat ctgcaaaagc cgtataggcc tccatattgg tataagccgt ttcacgactg
     2401 acctctacat aagaatcatg aacgcctgta attccaaagc tatggaatag tcgctgtgca
     2461 taagcaagtc cgtcaattcg ttccttgagg gtaatggctg gttcaaaact ctgtataagc
     2521 ggcatggcag attcttgaaa actaccagat ggttctccgg ttaagggatt acggtcaatg
     2581 cgacctccct caggatcctc tgtttctgcc gtaataccag cgcgttttat cgctgcggaa
     2641 ttcgcccaaa ccgtatgagc tgcgttggac agtaatgtga taggcgtatc aggtgctatg
     2701 gcatccaaga gttccttagg tggacgacct gtgggtgcga aagtactaac ataccaacca
     2761 gccccaacaa tccactcagc attcggtctc tcatcaacgc atgccctaat ggcatccaat
     2821 aggtcttgaa gctgaggcaa atcaaacaca gcacattgat tgaaggtcat tcctgaatcg
     2881 acaggatgca catgggcatc ctgaaaccct ggcataagca tcttgccgtc taactcgaca
     2941 acgcgtgtgt tagggccgat cgatgacgct gcacccacat catcaccaac ataggttatc
     3001 cttttttcgg taatagccac cgcggtcgcc caaggatccg tgtcagtcac ccggtaaacc
     3061 gcaccgccag taaaaacaac gtctgctcta ggagccttgt ccgatagttc gcaggcatta
     3121 agagtcataa gactcagagc aataaaaatt attttataca tgctgaaaat ggttagaatc
     3181 ctgcacagta gtaggcagat tggtcagcta cgtagacctg cggcgatcct ctttaggata
     3241 gcgtgtagtt tttcaagtcg atgccgcgtc aaggcatcaa catttgatcc tttagccgat
     3301 aacctatagc tattgatctg tcgggcaagt ctgttgttac caccgttatc cattgcctgc
     3361 gtgacttggt cgagcatatc cttgatccgt tctgcggcat cttcagcaat ggcgttatct
     3421 ctcagtagct gatcgatata agcacgcgcc accactggcg tcgcaggcca catcatgggc
     3481 acttgttgtt gagggttgaa cacacgacga ggtccaataa ttgggtaagc catagcagct
     3541 gcagcgatct cattctcact caaatactcg ctgggtatca gtttcagcgc gtctaggcct
     3601 cgggtgatct ctgttccata aatagtgccc tcgtaataat atacggacca ataacctccc
     3661 gtgatcaatt catcttctag tatggggccg cggtcaaagt aggcaatctc caagggttta
     3721 gaagaatcgg tgaaatcgat tactgagatt ccaccttggt accatgcctg cacgaaaatg
     3781 tcacggccag ggacagggac gatagaacca ttgtgagcta cgcagttttc cgtttccaat
     3841 tgcggagccg gcattttgta gtggcttcgg aattcaagtt tctcatctac gatgtcataa
     3901 atggcatcgg caccccagtt caaagggtcc caagcccgac agcgtgcacg tccaccccca
     3961 ccccattcgt ctgtgaacaa taccttggta ccttcattat tgaaggtggc cgagtgccag
     4021 taggcaaatc cggtatcagt taccacatcg atacgttcgg gttttcttgg gtcactaata
     4081 ttaaaaagga taccatttcc tgaacacgca ccagcagcta tgttggctga tgggaatacc
     4141 gttatatcat gacactggtc cgtaatctca gtctcctgcg aatcatcacc gtggtcacca
     4201 ccacgccaaa gaccagccag tacgccggtt tccgggtcag caaatacggc gggactgtca
     4261 atgatgctcg actgagaggg atcatccact ggaatttcga ttacatcaat gcggaataga
     4321 gcagtgcgat catcaccggg aatgtctttg acacattgat ccagttcttc ttcttctcga
     4381 acacttccag tgcccgagtt atagaccagg atcttgccat ctggagtagg acctgccact
     4441 accgagtggg tatgagaacc gcggcaagtt tgtactccgc ccacttgcat cgggcggctc
     4501 aaatctgaga tgtcgaagat acgcaatccc cggaaacggt ccaggctagc atctacggtt
     4561 acaccttcca ggccgcagtc aatgcggcca cgggtctgct cgactgacat aatcagtagg
     4621 tctccaacga ttgaaacatc accttgacct cctgggcaaa caacagaggt tatgagtttc
     4681 ggtagaccct cctcgtcgat gcgataaatg ttgaaaccgt ggtagctacc tgccaccagt
     4741 aggttgtcgc ggaaggccat gtccgtattg gaaaaactga gcattggata acgccgatct
     4801 tcggcagcct tttcgatggt ttgttcctta tcttcatcac ctgcctgttt gtccttgggc
     4861 tcatcgacac cacgttcttc gggatttgca gggtcataga atcccaaggg cttgctttgg
     4921 gatatcagta gttgcatatt catgatggct tcgccagcat cgtatagccc tgctttcagc
     4981 ccggcccggg gatcacttga aagacctatc agtagggcat tcattcgttc aatctccact
     5041 gcctgatcgt tggttacatt cgaggcgaat tcattgagaa ggggatcgta ggtggaaccg
     5101 ggttgttctc tgagttcttc aaccattttt atcgcaccat cgtgatgagt aatcatcagt
     5161 tgcaggtaaa gtcggtcaaa atctgtgctt tttgactcag agagctgagt catttgttct
     5221 ggagtcgcca tacccatcat gttgtggtga gtatgttctg agtggtcttc tacgagatca
     5281 ggcacttgct cttcgcgttc ttgtaaccag ctttgcataa aactgatttc atctgcctgt
     5341 gaaacatcga tgcgcccagc tagatctaga attgcttggt tattggtgct tggcgctgca
     5401 aactttgaca tcaccatagc ttggtgatga tgaataatca tgtcctgcat gaaatgggca
     5461 tcggcgaccg tgtatgaaga attggcgatg gccactgcgg tttcagcatc aatttcacga
     5521 gtagcattgc ctggagcgcc gggctggaca atcggaactg tcgacatgct aacactcggc
     5581 attaacaggc cacctaacag ggtgatgaga gtgactacgg aaaaagctct agaaagaaag
     5641 atgtagattg tcattgttag aggttactcc agcttaaaaa gtatacgaat taatattact
     5701 ataactggct atgcaccaaa gaggcataaa aaactgatag atgtatttat aaggttttat
     5761 aataactata gagatgaaaa aaattaatcc tgtcttaata aagatatatg tttgaattca
     5821 aagcaaaaaa agtattggtt actggagcat cacgtgggat tggtaaagcc atagcacaag
     5881 gttttgcaat gaaaggagct caagtatctt tggtttatcg aaaagaaaaa gagcttgctg
     5941 atgatctaag aaattcatta ccaggtgaga atcatatttg tgttcagtct gatgtgtctg
     6001 ttagtgaaga ggcacaatat gctgtatcaa caacacttga tgtatttgga tctctcgaca
     6061 tcgttataaa caatgccgga atgtatatag accatcctat agcagatgtt aattttgatg
     6121 attggcaaga cgcatggcag tcaaccatta atactaatct cattgggccc gctaatattt
     6181 gctttcatgc cgcaaaacac atgattaaga agggcagtgg aaaaataatt aatgtttcat
     6241 cacgtggagc aaatagagga gagccagtag ctccagctta cggtgcaagt aagtctggtt
     6301 tgaattctat gagtcaatca ttggctgttg cactagcccc atataacata tttgtaggag
     6361 ttgttgcacc tggctgggtg gatactgata tgacatctga atttttatct ggggaagaag
     6421 gggatgccat tcgaaaacaa agcccccttg gaagagtggg cacttctgaa gaaatagctc
     6481 aagcagtttt gatgctttct atggatggta tggaatatgc taccgggaca attatagata
     6541 taaacggcgc ttcttattta agatcttaag cttatagcaa tatgaaagaa ctaatcttga
     6601 tcactggtct actgctcatt ggtgggtatt tttcttttac tcccaatgct tcttttgcaa
     6661 accaccataa caaaatcgaa atcactgaaa cctggatctc gatgtcagat ggtatccggt
     6721 tagcagcaga tatttactgg cccgctgggg ctgatagaaa cgatcgtttc ccaattttgt
     6781 tagaatatct tccctatcga aaggatgaga gccgagctcg tagttattcc ctgtattctt
     6841 atttcttaga acacggttat cttgttgtcc gtgttgatat gcgaggtacg ggtaatagtg
     6901 aaggaatggt cattccttat gagtactcgg atatagaatt ggacgatggt gaagaggtaa
     6961 tcgactggtt gtcacaacag gaatggtcaa ccggtagcgt aggcatgttt ggcatctcct
     7021 ggggagggtt caactccata cagatggcta tgcgtaaccc gccagcctta aaagcattcg
     7081 tcgccttgat ggcgactgaa tttctatatc aagaagatgt ccactatatg gatggtatta
     7141 tgcatacgga ttcctggatg atgggcaatg acctctataa tgcgttgcca ggcgcacctg
     7201 atttcaagat ggatgaagaa tggttaagaa atcgtttcaa tgtaaaacca agtgtatata
     7261 aatatatgcg tcaacagcgt gatggtgaat tttgggatcg ggcctctgct cgtggaaaat
     7321 atgatttaat taaggtaccc ggctatcata ttggcggctg gtatgatgga tatcgcaaca
     7381 gcttaccgcg gatgttggaa aatgttcagg ctcctgtgaa agcaatgatt gggccgtggg
     7441 accatgattt tccacataat gcggcactgc aaccccaagt tgagtggcga gccgaggcag
     7501 ttcgttggtt tgatcaatgg atgaagggta tagatacagg gattctagaa gaaccagatt
     7561 ttgtagttta tattcgagat tatcatcctc ctgatgattc tctagagagc attccagggt
     7621 tttggcgatg ggaagagggt tggccaatca acagaattga aactcagact tggtatgctc
     7681 aagcaaatca taaattatca aatatctcag gagtaaatga caagcataca ttagagaaca
     7741 taccttccat tggcttggaa ggcggtggtc caactatgtg gtggggcagt attccatcgg
     7801 atcagcgccc tatggataaa aatagtttgg tatacgactc tgaggtactt gttgagccga
     7861 tagaaattct agggagacct ctagcaaaac ttcatgtatc cgctaatgca aaacgtgcta
     7921 actgggtagt tcgaatttct gatatcgcac cagatggcca aattactcag gtggcagggg
     7981 cagcttttaa tggaacacat agaaattctt cgcgtgaccc tgaagatata attcagggtg
     8041 aaaaattccc actcaatatt cagttacatt ttacatcatg gatctttaat gaaggtcata
     8101 aaattagagt ggcgatcagt aatgcaatgt ggccgatgtt atggccatct cctttaccag
     8161 taaaaacttt attagagatt ggtggagaaa aaggggcgta tatagagtta cccataatgc
     8221 cttctggtaa gataaaaaca cctgagtttg aacaactttc aataggacca aaattaaaag
     8281 gttatgaagt attagatgct ggcaatgtaa ctggttatgc tgcgatcaaa tcaattcaac
     8341 acgatccaga taccggtgaa aaatttggtc ttgcaaccaa tacaggagct actcagtacc
     8401 catggggcat tgagcgcttt gaagaagaga tagaacatcg aacatccgat gaaaatcctg
     8461 caaatacctc tgttgtgggg cgttacaaga tcactgagga attacaagat cggactcttg
     8521 atttcgaaca gaatgtagaa tttaaaagtg atgaagaaaa tttctatttg atattccatc
     8581 gttgggtgtc cgtcaatggt gaactctata aggagaaagt ttggcaggaa gttataccca
     8641 gggattttca gtagaagtag ctaagtaaca ttaatttggt aaataagaat ggttttaaat
     8701 aataattctt taatctgtta taactagaaa gatgaaaaaa cttatcttaa ttacaatctg
     8761 tttcacatca tgaaatacct tttagacagc ggcatgggtg atgaactgaa acagagagga
     8821 tttgaagtcc ctgattggaa aacttccatt tggtcagttt cagcgctcat caaatctccc
     8881 aatggagtag ttgagattca taaggacaat atcaaagctg gatgtaatgt gatcaccact
     8941 aacaattatt atgtcacacc aaacatactg aaaagagaag gtattgaaag tgaatttgag
     9001 agtctaacca gattggcagt tgacctagct gaacagtcaa gacaagggtt tcctgaagtt
     9061 ctaatagctg gagcctttcc acccatagag accagttttc ggccagacct tacacccagc
     9121 aacgaagtac ttgatgacta ttattcaaat cttggatcaa tcatccagaa aaaagtggac
     9181 ttgattctat gtgaaacaat gggctccatt agggaaggca tcagtgcagc aaggaatgcc
     9241 ttagaattta gcgaccgcgt ttggttgagt tggactaata gaggaatttc aggtgacata
     9301 ttacctagtg gagaacaatt aaaagacgct gtattagcag ccaatcaatt ggatattgaa
     9361 tgccagttag tcaactgttg tgctactgat ctggctacaa aaaatataga aatacttaaa
     9421 aaagcaacta attttgggat ctatgcaaac tctagaaaac tgaaagagca gaaatcaatc
     9481 caagatcttg gggaaactca agatatcgat gtaaatcact atgcaaatac agtacttatt
     9541 tcctctgagg agtatgcaag agaagctaaa atttggattg atcaaggtgc ttatgtggtt
     9601 ggtggttgct gtaccacaag accagaacat attaaaaaaa ttgcagatca attgaaataa
     9661 tagatcgagg ca
//
LOCUS       DASCTH010000034         2236 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000054663, whole genome shotgun sequence.
ACCESSION   DASCTH010000034 DASCTH010000000
VERSION     DASCTH010000034.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000054663"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="QGH35_00765"
     CDS             complement(<1..359)
                     /locus_tag="QGH35_00765"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HJL81096.1"
                     /translation="MTPYRNTIISIGSLLFVLLLSMSWIGGHASSAQKDLPKSLISIL
                     TEQAIPPETLTVIVQEVDSDESIISLNPKILRSPASLTKLFTTFIALDYLGPDFQWNT
                     NIYVSDSISDETVDYLL"
     gene            complement(373..1428)
                     /locus_tag="QGH35_00770"
     CDS             complement(373..1428)
                     /locus_tag="QGH35_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HJL81097.1"
                     /translation="MSYIKKPKILRSNAKRNDLGMTFSDYEGTMSTLCAGCGHDSITG
                     AIIEMVFDLSIPPHRMVKLSGIGCSSKTPAYFAKQSHGFNSVHGRMPSVATGAQAANS
                     GLHYIGVSGDGDTLSIGMGQFSHAIRRNLNMLYILENNGVYGLTKGQFSASADIGSKA
                     KKGAPNLQMPIDPCRVAISMGASLVARSFSGDKEQLVPLLKAGLMHRGFALIDVISPC
                     VTYNDHLGSTKSYLHTREHKQEAVYTDYIHPLSEVKVNYSAGSSIEVDLHEGGKVILS
                     KVARNYSPIDRAAVLKGIRESTEKGEILTGLLYIDESQADFIENENTIETPINQLAFE
                     DLCPGSEALSKLQKNYK"
     gene            complement(1425..>2236)
                     /locus_tag="QGH35_00775"
     CDS             complement(1425..>2236)
                     /locus_tag="QGH35_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013974.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HJL81098.1"
                     /translation="ADILSCAYASHGDTKHVCLYPANPEETFEFSKTAFDLAEQLQTP
                     VIVLTDLDIGMNDWMIKELQWDNKYQPERGKVLGAKKLEEIEEFHRYLDKDGDGIPYR
                     TIPGTHPKGSYFTRGSGHNELGGYTEDGEEYKSVMDRLLVKWESAKNSVPNPIIDCTK
                     SNDHAIVSIGSCDDAIREARDILGTKGILVNYLRVTAFPFSTEIEKFLAAHKRIFVVE
                     QNRDGQLYKLLQIESSVKQETLESIRIYDGMPLYAHMIVDEIEKRIKESKA"
BASE COUNT          654 a          490 c          407 g          685 t
ORIGIN      
        1 aacaataagt agtctactgt ctcatcagat attgaatcgg aaacataaat attagtattc
       61 cattgaaaat cagggcccag ataatccaaa gcaataaacg ttgtgaacaa ctttgtcaaa
      121 gatgctggac ttcttaagat ctttgggttc aaagaaataa ttgattcatc ggaatccact
      181 tcttgcacaa tgactgtcaa tgtctctggt ggaatagctt gctcggttag gatacttatc
      241 aggctttttg gcaaatcttt ttgcgcggaa gaagcatgtc caccaatcca ggacatactc
      301 aacaacaaga caaacaataa gctacctata ctgatgatgg tatttctata tggcgtcact
      361 aaaaaggggg gtttatttat agtttttttg gagctttgat aatgcctcgg agccaggaca
      421 caagtcttca aaagcaagtt ggttgatcgg ggtttcaatt gtattttcat tttcaataaa
      481 atcggcctga ctttcatcaa tatagagcaa acccgttaat atctcacctt tctcggtcga
      541 ctcgcgaatg cctttaagaa cagcagcccg gtctatggga ctgtagtttc tagcgacctt
      601 gctaagaatg accttacccc cttcatgcaa atcaacttcg atggaagatc ctgctgagta
      661 attcacctta acctcactta gagggtgaat ataatcggta taaaccgctt cttgtttatg
      721 ttctcttgtg tgtaaataac ttttggttga gcctagatga tcattgtatg tgacacatgg
      781 cgatatcaca tcgatcaaag caaaacctct atgcattaag cctgctttaa gtaagggtac
      841 taactgttcc ttgtcacctg aaaaactccg agcaaccaaa gaagctccca tggaaatagc
      901 cacccgacat ggatcaatgg gcatttgcag attaggagct cccttcttag ccttggaacc
      961 aatatctgca gaggctgaaa actgcccctt agtcagtcca tagacaccat tgttttccaa
     1021 aatgtagagc atgttcagat tgcgtctaat cgcatgtgaa aattgtccca tgcctatgga
     1081 aagtgtatcc ccatctcctg aaacgccaat gtaatgaagt ccactgtttg cagcctgtgc
     1141 tcccgttgca actgagggca ttctgccatg aacggaatta aagccatgag actgctttgc
     1201 aaaataggct ggggttttag aagagcaacc aatgccactc aatttaacca tcctgtgtgg
     1261 tggaatactc aaatcaaaga ccatttcaat aattgctccc gtaattgagt catgtccaca
     1321 acctgcacac aacgtcgaca tagttccttc gtaatcggaa aaggtcattc caagatcatt
     1381 gcgtttcgca ttgcttctca aaatttttgg ctttttgata taactcatgc tttactttct
     1441 tttatcctct tttctatttc atcaacaatc atgtgtgcat acaaaggcat accatcatat
     1501 attctaatag attctaaggt ctcttgttta acgctagact caatttgtaa caatttatac
     1561 aactgaccat cgcgattctg ttcaacgaca aatatcctct tatgagctgc taaaaacttt
     1621 tcgatttcag tagaaaatgg gaatgcagtc actcttaaat aatttaccaa tatgcccttt
     1681 gtgcctagaa tatctctggc ttcacgaatg gcatcatcac aactacctat agaaacaatg
     1741 gcatgatcat tggatttggt acaatcaata atggggtttg gtacagaatt ttttgccgac
     1801 tcccatttaa ctagaagtcg atccataacg ctcttatatt cctccccatc ttcggtataa
     1861 ccacccaatt cgttgtgtcc agaacctctg gtaaaatagg aacccttggg atgcgtgcca
     1921 ggtatagtcc tatatggaat gccatcaccg tccttatcta aatatctgtg aaactcttca
     1981 atctcctcca atttttttgc acctaaaact ttgcctcttt cgggttggta tttattatcc
     2041 cattgtaatt ccttaatcat ccaatcattc atgccaatat caagatcggt aagcactatg
     2101 acaggggtct gtaattgctc agctaagtcg aaagctgttt tggaaaattc aaatgtttct
     2161 tccggatttg cagggtataa acagacatgt ttggtatccc cgtgagaggc ataagcacac
     2221 gataaaatat cagctt
//
LOCUS       DASCTH010000035         1956 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000055185, whole genome shotgun sequence.
ACCESSION   DASCTH010000035 DASCTH010000000
VERSION     DASCTH010000035.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000055185"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="QGH35_00780"
     CDS             complement(<1..218)
                     /locus_tag="QGH35_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017943854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81099.1"
                     /translation="MEQVLENVGFIQNGVILPGFIFGVVHVGLVILGFYTGWSINRLL
                     KNITKGYIAGIIGAGMAHVLADLIAAIV"
     gene            257..>1956
                     /locus_tag="QGH35_00785"
     CDS             257..>1956
                     /locus_tag="QGH35_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014896.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HJL81100.1"
                     /translation="MKDLSIKCYFVSACLIVTLYATLGHTKNNEYPFSKTTDVSDHYH
                     GQAIADPYRWLEDLNSKETKDWIKQQNQFSQNYLHDVKQKQSIKNILIKAYDKETRSV
                     PFRVQSKTFFYFNSGEWQQSKLFFRDTHQEKQQLVLDPNTFSDDGTIALGGVDVSPNS
                     ELIAYAITDGGSDWKTWKIRNIKTGKDLKDSIAWSKFSNAEWAKDNSGFYYSAYEKPK
                     AGDEYESLNTSQRLFFHKLGQNQTEDQLIFERPDHPEWGFGATVSEDGKYLVLSISEG
                     TDERNRIFYKDLSNDNSDFIELIGELKAQFSFVGNEGTIFWFFTDLNAPMGRVIAIDI
                     NQSEEENWHELIPEKEYPLTNVRELKDFLLCQYLVDVTSRINLYQKKGHLEKTMQFSK
                     TGTISSLSAQPETNTFYFSFSNYILPNEIFKYDASLDTLNTIWKRKVPGYNADEYVSN
                     KVFYASKDGTLIPMFISHQKGISFNHDNPLLLYGYGGFDISILPGFRERYFAWMKMGG
                     VVAIPNLRGGGEYGEQWHQQGMLHNKQNVFDDFAAAAEYLHREGYSRPDLTTIEGRSN
                     G"
BASE COUNT          661 a          390 c          374 g          531 t
ORIGIN      
        1 tcaacaatgg ccgctatcaa atcagcaagg acatgagcca tgccggcacc aatgatccca
       61 gcaatgtatc ctttggtaat attttttaat aaacggttga tgctccagcc tgtataaaag
      121 cccaaaataa caagaccaac atgaactaca ccaaatataa agccgggcaa tatgaccccg
      181 ttctgaatga agcctacgtt ctcaagtacc tgttccatgg tgttactcca aagatgtata
      241 atctttaata aaaattatga aagatctatc catcaaatgc tattttgtat ccgcttgtct
      301 tatagtaact ctatatgcaa ctctaggaca cacaaaaaac aacgagtatc cattttctaa
      361 aactactgat gtctctgatc attatcatgg acaagcaatc gcggatccct atcgatggct
      421 ggaagacctt aattccaaag aaaccaaaga ttggatcaag caacaaaacc agtttagcca
      481 aaactatttg catgatgtca agcagaagca aagcattaaa aacattctca tcaaggccta
      541 cgacaaagaa accagatctg ttccatttcg tgtgcaatct aaaactttct tttatttcaa
      601 tagtggagaa tggcaacaaa gtaaattatt ttttagggac actcatcaag aaaaacagca
      661 attggttcta gatccaaaca cattttcaga tgatggaact attgctcttg gtggtgtcga
      721 tgtcagccca aactcagaat taatagccta tgccattacc gatggtggct ctgattggaa
      781 aacttggaag ataagaaata tcaaaacagg caaggattta aaagacagca tcgcttggtc
      841 taaatttagc aatgcagaat gggccaaaga caactctggt ttctattact ctgcttatga
      901 aaagcctaaa gcaggcgatg aatatgaaag cctgaatacc agccaaaggt tgttttttca
      961 taagcttggt caaaaccaga ctgaagacca gttaattttt gaacgcccag accatccaga
     1021 atggggcttt ggagcaacag tgagtgaaga tggcaaatac ctggtgctta gcatcagtga
     1081 agggacggat gagagaaata gaatctttta taaagatctg tctaatgaca acagtgattt
     1141 catagaactc attggagaac tcaaagccca atttagtttc gttggaaatg agggtactat
     1201 cttttggttt tttactgatc tcaatgcgcc aatgggcaga gtcatagcta tagatattaa
     1261 tcaatcagaa gaggaaaatt ggcatgaact gattcctgaa aaagaatacc ccttgaccaa
     1321 tgtcagggag ctaaaagatt ttctactctg tcagtatttg gtcgatgtaa ccagtcgaat
     1381 caatctgtat caaaaaaaag gacaccttga gaaaacaatg caattctcca aaactggaac
     1441 tatctcaagt ttgtctgcac agcctgagac caatactttt tacttctcat tttcaaacta
     1501 cattctaccc aatgaaattt ttaaatatga cgcctcccta gatactctca acaccatttg
     1561 gaagagaaaa gttccaggtt acaatgcaga cgaatacgtc agcaataagg ttttctatgc
     1621 aagcaaagac gggactctga ttccgatgtt catttctcac caaaaaggga tctcattcaa
     1681 tcatgacaat cctttattgc tttatggata tggtggtttt gatatttcta tcctacctgg
     1741 atttcgagaa agatattttg cttggatgaa aatgggtggt gtggttgcca tacccaatct
     1801 tagaggtggc ggtgaatacg gtgaacaatg gcaccaacaa ggaatgttgc acaacaaaca
     1861 aaatgtgttc gatgactttg cagcagctgc agaatatctc cacagagaag gctattcgag
     1921 accagatctg accaccattg aaggacgttc caatgg
//
LOCUS       DASCTH010000036         5295 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000055862, whole genome shotgun sequence.
ACCESSION   DASCTH010000036 DASCTH010000000
VERSION     DASCTH010000036.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000055862"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /gene="fdhF"
                     /locus_tag="QGH35_00790"
     CDS             <1..2084
                     /gene="fdhF"
                     /locus_tag="QGH35_00790"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HJL81101.1"
                     /translation="CSFKAEIKNNELIRMTPQKDGKANHGHSCIKGRFAWGYTNHQDR
                     IRQPMLRENITDPWQIVSWQKAIEFSASKLLKIQKEFGANSIGGITSSRTTNEEVYVV
                     QKLIRTAFGNNNVDTCARVCHSPTGYGLKQTFGTSAGTQNFDSILESDVIVVIGANPT
                     VAHPVFASQIKKRVRQGARLIVIDPRRTEIVQSPHIKAEYHLPLLPGSNVPLMNAFSH
                     IAVKEGLIDYEFINERCDIDSFDSWKSFILSDTHAPKNIEAVTGLDASTITAAARLYA
                     SGGKGSIYYGLGVTEHSQGSTMVMAMANLAMATGNIGKSGCGINPLRGQNNVQGSCDM
                     GSFPHELPGYRAVEDNKVREIFEKKWQVSIQSHPGMRSVNMFDEAVVGTFKGIYIQGE
                     DFAQSEPNIQHIEQAFKSMECVIVQDLFLTETASYAHVFLPATSFLEKDGTFTNAERR
                     INRVRPIMEPITGKHEWEITQELSQAMGYDMNYSSSSEIMDEISELTPSFNGVSFDKL
                     DQLGSIQWPCNKEAPEGTTIMHVDRFVRGSGKFIITEFVPTTEVANRRFPLLLTTGRK
                     LTQYNVGTQTRRTPNNIWGMDDVLEIHPIDASTRGISDNSLVLLQSRKGETTLKALVT
                     EDIAPGVVYTTFHNPDTGANVVTTENSDWATNCPEYKVTAVEISPAEHRSNWQINRTK
                     KLEQLRQIINS"
     gene            2090..4006
                     /gene="thrS"
                     /locus_tag="QGH35_00795"
     CDS             2090..4006
                     /gene="thrS"
                     /locus_tag="QGH35_00795"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HJL81102.1"
                     /translation="MPSITLPDGSKKDFEQSISIDDLAKSIGPGLARSAIAGKINETL
                     YDLSYVIEKDCNASIITKETSEGLDIIRHSTAHLMAHAVKLLYPKVEVTIGPVIENGF
                     YYDFAIDTPFTDQDLESIENKMHEIAKQNLCISREVWTKDQALKYFKKNNENYKAEII
                     KDIADEDDLSVYKQGDWLDLCKGPHVPSTKFIDSFKLTKVAGAYWRGDSSNEMLQRIY
                     GTAWSDKKSLKQYLVQLKETKKRDHRRIGKDLDLFSIQEEAGGGLVFWHPNGSRIRHV
                     IEDYWHQKHLEANYELLHTPHIALDTLWQTSGHTDFYEELMYKPIEDENQLYRLKPMN
                     CPFHVLIYKNQLRSYRDLPIKWAELGTVYRNEMTGALHGLMRVRGFTQDDAHVFCTED
                     QTETEIANILDLTIEMLSAFTFDQYEIHLATQPDKSVGSKAIWNKATKALKSALTSRN
                     LEYSLDEGGGAFYGPKIDIKIKDAIGRLWQCTTIQVDFNLPERFDMNYIGQDGKKHHP
                     IMIHRALLGSIERFFGILIEHFEGKFPLWLAATQLMILTISKSQKDYAQSIYKKLKNN
                     GFRVEIDLRNEKIGSKIRDHTLKRIPYLIIVGDQEVKNNKISVRTQKGDDLGNLDCDD
                     FINKLSKEILDKSN"
     gene            4022..4540
                     /gene="infC"
                     /locus_tag="QGH35_00800"
     CDS             4022..4540
                     /gene="infC"
                     /locus_tag="QGH35_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013818389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HJL81103.1"
                     /translation="MAAKKIVRRNEQIDSSEIRLIDNDGSQIGIINIKEAIELARSKG
                     MDVVEVSPNTSPPVCRVMDFGKFVFEKKKKSQGAKKKQKIIHVKEIKFRPLIEDGDYN
                     VKLGKILKFLEQGDKVKISLRYRGRELRHAELGENLLKRVCDDIDHIATIEQEAQREG
                     RQLIMIVAPKHN"
     gene            4572..4769
                     /gene="rpmI"
                     /locus_tag="QGH35_00805"
     CDS             4572..4769
                     /gene="rpmI"
                     /locus_tag="QGH35_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015464854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HJL81104.1"
                     /translation="MPKLKTNRGAAKRFKKTGTGKYKRSSSHMNHILTKKSSKRKRHL
                     RSSSLISAGDLKSVKKLLPYS"
     gene            4785..5147
                     /gene="rplT"
                     /locus_tag="QGH35_00810"
     CDS             4785..5147
                     /gene="rplT"
                     /locus_tag="QGH35_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010547156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HJL81105.1"
                     /translation="MARVKRGVTSHAKHKKVLKKVKGHYGARSKLIKVAKQAAIKAGQ
                     YAYRDRRKKKNQFRSLWIIRINAAARMCDISYSKLMHGLKVANIDIDRKILADLAVND
                     EDAFRALTDKAKLALEKS"
BASE COUNT         1971 a          873 c         1026 g         1425 t
ORIGIN      
        1 gttgttcatt taaagctgaa ataaaaaata atgagctcat aagaatgact ccccaaaaag
       61 atggcaaagc aaatcatggg cacagctgta ttaaaggcag gtttgcttgg ggctatacca
      121 atcatcagga tcgtattagg cagccgatgc taagagaaaa cataacagat ccttggcaaa
      181 ttgttagttg gcaaaaagca attgaatttt cagcatcaaa attattaaag attcaaaaag
      241 aatttggtgc caatagtatt ggtggcatta cttcatcaag gactactaat gaagaagtat
      301 atgttgtaca aaaactaatc agaactgcct ttggtaacaa taatgttgat acatgtgcaa
      361 gagtttgtca ttcacctact ggttatggct taaaacaaac ctttggaaca tctgcgggaa
      421 ctcaaaattt tgatagtatt cttgagtctg atgtcattgt agtgattggt gctaatccca
      481 ctgtggctca cccagttttt gcttcccaga ttaaaaaaag agttcgacaa ggtgccagac
      541 tcatagttat tgaccctaga cgaactgaga ttgttcaatc accccatata aaagcagaat
      601 atcatttacc tttattacca ggttccaatg tcccattaat gaatgctttc tcccatattg
      661 ctgtcaaaga aggactgatt gattacgagt ttattaatga aagatgcgat atagactcat
      721 ttgatagttg gaagtcattt attttaagtg acacccatgc tcctaaaaat attgaagcag
      781 taacaggtct agatgcatcc actataacag cagcagcaag gctatatgca tctggaggga
      841 aaggttcaat ttattatgga ttgggtgtca cggaacattc gcaaggttct acgatggtta
      901 tggctatggc caatttagca atggcaacag gtaacattgg caaatcagga tgtggaatta
      961 atccattacg tggacagaat aatgttcagg gatcttgtga tatgggatcc ttccctcatg
     1021 aattgccagg ctacagggca gtcgaagata acaaagtcag agaaattttt gaaaaaaaat
     1081 ggcaagttag tattcaaagt catcctggga tgagatcagt gaatatgttt gacgaggctg
     1141 ttgtcggcac tttcaaaggc atttatatcc aaggagaaga ctttgctcag tcagaaccaa
     1201 acattcaaca tattgaacaa gcatttaaat ctatggagtg tgtgattgtg caagatcttt
     1261 ttttaactga aacagcaagc tatgcccatg tatttctacc agctacttcg tttcttgaaa
     1321 aagacggaac tttcaccaat gctgaacgtc gcatcaatcg agtaaggcca atcatggaac
     1381 ctatcacggg taaacatgaa tgggagatta ctcaagaact tagtcaagct atgggatacg
     1441 atatgaatta ttcatcttct tcagaaatta tggatgagat ttcagagcta actccaagtt
     1501 ttaatggggt ctcatttgat aaattagatc aactaggaag cattcaatgg ccatgcaata
     1561 aagaagcgcc agaaggtacg acaatcatgc atgtggatcg atttgtaagg ggttctggta
     1621 aatttataat tactgagttt gtgccaacaa ctgaggtagc aaacagaaga tttccattgt
     1681 tgttaaccac cggaagaaaa ctaactcaat ataatgttgg gacccaaact aggagaacac
     1741 caaataatat ttggggaatg gatgatgtgc tagaaataca tcccatagat gcatctacac
     1801 gcggtataag tgataatagt ctagttctac tgcaaagcag aaaaggagaa acaacattga
     1861 aggcattggt tactgaagat attgcacctg gagttgtcta tacaactttt cataatcctg
     1921 atacaggcgc aaatgtcgtg acaaccgaaa atagtgactg ggcaaccaat tgtcctgaat
     1981 ataaagtaac tgcggtggag atcagcccag cggaacatcg ttcaaactgg caaatcaaca
     2041 gaaccaaaaa acttgaacag cttcgtcaga tcataaactc ttaaatatta tgccttctat
     2101 tactcttccc gatggtagta aaaaagattt tgagcaatct atttcaatag atgatcttgc
     2161 taaatctatc ggtccgggtt tggctaggtc tgcaatagct ggaaaaatta atgaaacact
     2221 atatgactta tcatacgtca ttgagaagga ttgtaatgct tcaataatca caaaagaaac
     2281 atcagaagga ctcgatataa taagacattc aaccgcacac ttaatggctc atgcggtcaa
     2341 attactatac ccaaaagttg aagtaactat cggtccagtg attgaaaatg gattttatta
     2401 cgatttcgca atagacactc cattcactga tcaagaccta gaaagcattg aaaataaaat
     2461 gcatgaaatt gctaaacaaa atttatgcat ctcaagagaa gtatggacaa aagaccaagc
     2521 cttaaaatat ttcaaaaaaa ataatgaaaa ttataaagca gaaattatta aagatatcgc
     2581 agatgaagat gacttatcag tctataaaca gggtgattgg ctggatttat gtaaaggacc
     2641 ccatgttcct tcaacaaagt tcattgattc ttttaaactg accaaggttg caggcgctta
     2701 ttggagaggt gattcttcaa atgaaatgct tcaaagaatt tacggaaccg catggagcga
     2761 caagaaatca cttaaacaat accttgtcca actaaaagaa acaaaaaaaa gagaccatag
     2821 aagaattggg aaagatcttg atttatttag tattcaagaa gaggcgggag gtggtctagt
     2881 cttttggcat cctaatggat caagaatcag acacgtcata gaagattatt ggcatcaaaa
     2941 acatttggaa gcaaattatg agcttttaca cactccccat atagctcttg atacattatg
     3001 gcaaacctct ggtcatactg acttctatga agaattaatg tacaaaccca tcgaagatga
     3061 gaatcaactg tatcgcctta aaccaatgaa ttgtccattt cacgtactaa tctataagaa
     3121 tcaattaaga tcctatcgtg acctacccat taagtgggct gagctgggaa ctgtttatcg
     3181 taatgaaatg acaggtgctc tgcatggatt aatgcgcgtg agaggattca ctcaagacga
     3241 tgctcatgtt ttctgtactg aagatcaaac tgaaactgaa attgccaata tcctagattt
     3301 aacaatagaa atgctaagcg ctttcacttt tgatcaatat gaaatccatt tagcaactca
     3361 accagataaa tcagttggga gcaaagcgat atggaataaa gcaactaagg ccttaaaaag
     3421 tgctctaact tcaagaaatc ttgaatattc attagatgaa ggaggagggg cattctatgg
     3481 acctaaaatt gatattaaga tcaaagatgc gattggtaga ctctggcaat gcacgacaat
     3541 tcaagttgat tttaaccttc ctgagcgatt tgatatgaac tatattgggc aagatggcaa
     3601 gaaacatcat cccatcatga ttcaccgagc attattgggc tccattgaac gtttttttgg
     3661 aatattaatc gaacattttg aaggaaaatt tccattatgg cttgctgcta ctcaactcat
     3721 gatccttaca atcagcaaat ctcagaaaga ttatgctcaa tcaatttata aaaaattaaa
     3781 aaataatggc tttagagtcg aaattgactt gagaaacgaa aaaataggct ctaaaattcg
     3841 cgatcacaca ttaaaaagaa taccgtattt aattattgta ggtgatcaag aagttaaaaa
     3901 taataaaata tcagttcgca cccaaaaggg agatgatcta ggtaacctgg actgtgatga
     3961 ttttattaac aaattgtcaa aagaaatact agataaaagc aactaaaaga ggagatttac
     4021 tatcgcagca aaaaaaattg tcagaagaaa tgaacaaatt gattcatctg aaattcgctt
     4081 aattgacaat gatggaagtc aaataggaat aataaatatt aaagaagcca ttgaattagc
     4141 acgttccaaa ggaatggatg tggtagaggt tagccccaat acctcaccac ctgtttgcag
     4201 agttatggat tttggtaagt ttgtttttga aaagaaaaag aagagtcagg gtgcaaaaaa
     4261 gaaacaaaaa attatacatg taaaggaaat taaatttaga cccttaatcg aagacggtga
     4321 ttacaatgtt aaattaggga aaatattgaa attcttagaa caaggagaca aagtaaaaat
     4381 atccctcaga tatagaggta gggaacttag acacgctgaa ctgggtgaaa accttctaaa
     4441 gagagtgtgt gatgatattg atcatatagc tacgatagaa caagaagcac aacgtgaggg
     4501 tagacaactg attatgatag ttgcgccaaa acataactaa ttaaaatggt gaaaaagaaa
     4561 aggtaaataa catgccaaaa ttaaaaacaa acagaggtgc tgctaaacgg tttaaaaaaa
     4621 ccggaaccgg taaatataag agaagttcat cacacatgaa tcatatactt acaaaaaaaa
     4681 gcagcaaacg aaaaagacac ttaagatcat cttctctcat atcagctggt gatcttaaga
     4741 gtgttaaaaa attattacca tatagttaag ataaggaatt aaaaatggcc agagtaaaaa
     4801 gaggggttac ttctcatgca aaacataaga aagtcctaaa aaaagtaaaa ggacactatg
     4861 gtgctagaag taaactgata aaagttgcaa aacaagccgc gatcaaagct ggacagtatg
     4921 catatagaga tagacgcaag aaaaaaaacc agtttagatc tctatggata attagaatta
     4981 atgccgctgc tagaatgtgt gatatctcct acagtaaact catgcatggc ttaaaagtag
     5041 caaatattga tattgatcga aaaatactag ctgatttagc cgtcaacgat gaagatgctt
     5101 tcagagcact cacagataaa gcaaaacttg ccttagaaaa gtcataatga cgattaactt
     5161 gtcagaccta ttaaatgatg cacttaatga aatagaagaa tgcaaaacac ttaataagct
     5221 tgaagaaata agagttaagt atttaggtaa aaaaggtcaa attacctccc aattaaagaa
     5281 attgggctca atgaa
//
LOCUS       DASCTH010000037         3941 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000056053, whole genome shotgun sequence.
ACCESSION   DASCTH010000037 DASCTH010000000
VERSION     DASCTH010000037.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000056053"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="QGH35_00815"
     CDS             <1..548
                     /locus_tag="QGH35_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HJL81106.1"
                     /translation="PTFERTFDQIGLHEDGYSTVDLTTSVMQTLSDREKEIIQMAVDN
                     IYLKFISKVGENRSMSNEEVDAIARGRVWTGEKALEIGLIDELGDIEDAIAAAGNLAE
                     LDDYGVKLIEREMDFGFNFGFSIDNDRMSKMISFLDFLGFSTASDSLPPIIKTFEQQS
                     NLLNNFNDPRGMYYYCFCSVE"
     gene            complement(551..1099)
                     /gene="sufT"
                     /locus_tag="QGH35_00820"
     CDS             complement(551..1099)
                     /gene="sufT"
                     /locus_tag="QGH35_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018870138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HJL81107.1"
                     /translation="MLDRDEVITLSRDCETISVPYGEKNTLKKGTEVQIMQAMGGSYT
                     VYTSEGMFRINGYNADAIGKETEPPPFVPKNISDEEYESKIWEQMKTVYDPEIPINVV
                     DLGLIYTCEIKKNDTNEYSVVIEMTLTAPGCGMADVLVEDIKERVEMLPRIAKVEVAL
                     VLDPPWSMEMMSETARLEAGLI"
     gene            complement(1092..1550)
                     /locus_tag="QGH35_00825"
     CDS             complement(1092..1550)
                     /locus_tag="QGH35_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HJL81108.1"
                     /translation="MNQELATLYQTTILDHGHHPRNCGKPKKYDQKLEGFNHLCGDKI
                     MIYCNCSRHENIKISFESVSCVICTASASLMTTELEDASRQQAIALIDEVLNGLNNAS
                     LCSKELSDQLLGLSGVVKFPSRIQCATLPWETTRKIILDSGSFNNGESGA"
     gene            complement(1551..2786)
                     /locus_tag="QGH35_00830"
     CDS             complement(1551..2786)
                     /locus_tag="QGH35_00830"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011945767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HJL81109.1"
                     /translation="MSSNNNDEFSPDLLRKDFPILDQEINGAPLAYLDNAASTQKPKA
                     VIEAIGNYYNKDHSNVHRGVHTLSVRATELYEQSRSKIAQFINSNSSNEIVFTRGTTE
                     SINILASSLSKSINKGDEILISTMEHHSNIVPWQELSQKTGASLKIIPINKLGEIQLD
                     QLESMLNNHTKLISMTHTSNTLGTINPIKDIIKMAHLQDIKVIIDGAQAIAHQNIDVQ
                     ELDCDFYAFSGHKLYGPTGIGVLYGKENLLNQLPPYQFGGEMILKVSFEHTTYNSLPY
                     KFEAGTPNIAGSIGLGSAIDYLKSIPFDLCQQHEMRLHNYALEKLKSVDRLKLIGESS
                     EKSPIISFIIENIHPHDIGTILNQKGIAVRTGHHCTMPLMDYFSIAGTIRASFSFYNT
                     IQEIDQMIDALDTACEMLA"
     gene            complement(2810..>3941)
                     /gene="sufD"
                     /locus_tag="QGH35_00835"
     CDS             complement(2810..>3941)
                     /gene="sufD"
                     /locus_tag="QGH35_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HJL81110.1"
                     /translation="AIPSKKDEKWQYTDIEPYIEKCFELFAKRNVHKKSKKSKPLMEK
                     NNDKDIIIYYQNGYFFCRQNLPNYIKTLCTSENPETFKSLLDRKSSNLISDLNILMLS
                     DVLVIETLKNQKIDDIIHIVICSDNEKNINPRFIFKLQENSSITIFQHQLASHDSVTN
                     GLSDINCGDHSSLELYKIIDENDNGYQIDTQNITLAKNSRLNLFTLDLGAEISRTNIN
                     ANLVEKNASINIHALFSSRNKLHIDNQIMIKHIAEECRSSMNYRGIMQDNSTGVFGGT
                     VYVDKNAIKTQSDMTNRNLLLSDNARINSKPVLEIYNDDVQCSHSATSGHLDYDKLFY
                     IQSRGVCEKEARDILIQSFSDEFINKITNRRIRNVFQETITQ"
BASE COUNT         1122 a          670 c          725 g         1424 t
ORIGIN      
        1 tacctacctt cgagagaact tttgatcaaa ttgggcttca tgaggacggt tattcaacag
       61 tcgatctgac tacctctgta atgcaaactt tatcagatag ggaaaaagaa ataatccaaa
      121 tggcggttga taatatttat cttaaattca tctctaaggt gggtgaaaac agatccatgt
      181 ctaatgagga agttgatgct attgctaggg gtagagtatg gacgggcgag aaggctttag
      241 agatcggact cattgatgaa ttgggtgata ttgaggatgc aatcgctgca gcaggaaatc
      301 tggctgaact agatgattat ggagtcaagt tgattgaaag ggagatggat tttggattta
      361 attttggttt ttctattgat aacgatcgga tgtccaagat gatatctttt ttggattttc
      421 ttggattctc cacagcttct gactcattac ctcctattat taaaaccttt gagcagcaat
      481 caaacctact gaataatttc aacgatccta gaggcatgta ttattactgt ttttgttcag
      541 tagaataaaa ctagatcaaa cccgcctcta atctggcggt ctctgacatc atctccatac
      601 tccaaggtgg gtctaggacc aatgctactt ccactttggc aattctcggt agcatttcta
      661 ctctttcttt gatgtcttca accagtacat ctgccatacc acaacctggg gcagttaaag
      721 tcatttcaat gacaacggag tattcattag tatcattctt tttaatttca caggtataga
      781 tcaggccaag gtcaacgaca ttgattggta tttcagggtc atagacagtt ttcatttgtt
      841 cccagatttt tgattcgtat tcttcatctg atatattttt tggtacaaaa ggtggcggtt
      901 cagtttcttt accaatcgca tctgcattat agccatttat cctgaacata ccttcactgg
      961 tataaaccgt gtacgaacct cccattgctt gcataatttg aacttctgtt cctttcttca
     1021 aggtattttt ttcaccataa ggaacggaga ttgtttcaca atcccttgaa agcgtaatga
     1081 cctcgtcacg atcaagcacc gctttctccg ttattaaatg atccagagtc taatattatt
     1141 tttcgggttg tttcccaagg tagggtggcg cattgaattc ttgatgggaa ttttacaaca
     1201 ccactgagac ccaagagttg atctgacagt tcttttgaac ataaagatgc attatttagt
     1261 ccgtttaata cctcatcgat caatgcaatt gcttgttgcc ttgaagcatc ttccagctct
     1321 gttgtcatca gagaagcaga ggcagtacag attacacaac tcacgctttc aaatgagatc
     1381 ttaatgtttt catgtcgact gcaattgcaa taaatcatga tcttatcacc acaaaggtga
     1441 ttaaatcctt ccagtttttg atcatatttt tttggttttc cacagtttct aggatggtgg
     1501 ccgtgatcca gtatggttgt ttggtacaat gttgctaatt cttgattcat ttaagcaagc
     1561 atttcacaag cagtatccaa ggcatcaatc atttggtcta tttcttggat cgtattataa
     1621 aaactaaacg aagctctgat agttccagca attgaaaaat aatccattag cggcatcgta
     1681 cagtgatgtc ctgttctaac agcaatacct ttttgattta atattgttcc aatatcgtgt
     1741 ggatggatgt tttctataat gaaggaaatt attggtgatt tttcactgct ttcccctatc
     1801 agcttgagcc tgtcaactga ttttaatttt tccagagcat aattatgtaa tctcatttca
     1861 tgttgttgac acaaatcaaa gggaattgat tttagatagt caatggcact gcccagtcca
     1921 atggagccag ctatattagg tgtacctgcc tcaaacttat atggaagtga attataggtt
     1981 gtatgttcaa agcttacttt taatatcatc tcacctccaa attggtaagg agggagttga
     2041 ttcagtaggt tttcttttcc atataaaaca ccaattccag ttggaccata tagtttgtga
     2101 cctgagaatg catagaaatc acaatctaat tcttgaacat caatattttg atgagcaatg
     2161 gcttgagcac cgtcaatgat gacttttata tcttgaagat gggccatttt aatgatgtcc
     2221 ttaattggat taatggtacc taatgtattt gaagtatgag tcattgaaat taactttgta
     2281 tgattattta acatgctctc caactgatcc agttgaattt ctccaagttt attgattgga
     2341 atgatcttta aactggcacc ggttttttga ctgagttctt gccagggaac gatattggaa
     2401 tggtgttcca ttgttgagat taaaatttca tctcctttgt tgatagattt tgatagagag
     2461 ctcgctagga tattaattga ttcggttgtt cctcttgtga aaacaatttc attggaagag
     2521 tttgagttga taaattgagc aatctttgat cgtgattgct catataattc tgttgctcta
     2581 acactcagag tgtgaacacc tcggtggaca tttgaatggt ctttattgta ataattaccg
     2641 attgcttcaa tgacagcttt tggtttttgt gtagatgccg cattgtctaa ataagctaac
     2701 ggagcgccat taatctcttg atctagtatt gggaaatctt ttcttaagag atccggacta
     2761 aattcatcat tgttattgct tgacatgatc ttattgctca tcgttctatt tattgtgtaa
     2821 tagtttcttg aaagacattt ctgattctac ggttagtaat cttattaata aattcgtcag
     2881 aaaaagattg aattaagata tctcttgcct ctttttcaca gacacctctt gattgaatat
     2941 aaaagagttt gtcatagtct aagtggcctg atgttgcact atgtgagcat tgaacatcgt
     3001 cattatagat ctctaaaaca ggtttactgt taattctggc attatcggat agaagtaagt
     3061 ttctattggt catatcggat tgagttttta ttgcattctt gtcaacgtat acagttccgc
     3121 caaatacgcc agttgaatta tcttgcataa ttccacggta gttcattgaa ctacgacatt
     3181 cctctgcaat gtgtttaatc attatttgat tatctatgtg caatttattt cttgaggaga
     3241 atagggcatg gatattgatg gatgcatttt tttcaaccaa gttagcattg atatttgttc
     3301 ttgaaatttc agcacccaaa tccaaagtaa agagatttag acgactattt ttagctaatg
     3361 taatattttg agtgtcaatc tgatacccat tatcgttttc atcaattatt ttatatagct
     3421 ctagtgagga atggtctcca cagtttatat ctgataagcc gtttgtaaca gaatcgtgtg
     3481 aagcaagttg gtgttgaaag atggttattg aactgttttc ttgtaacttg aaaatgaacc
     3541 taggattaat atttttctca ttatcagagc aaataacaat gtgaataata tcgtctatct
     3601 tttgattttt aagcgtttcg ataacaagta catcagacaa catcagtata ttaagatcgc
     3661 taataaggtt tgaagatttc ctatccaata atgatttaaa agtttcagga ttttctgatg
     3721 tacacaatgt ttttatgtag ttgggtaagt tttgccgaca aaagaaatat ccattttgat
     3781 agtaaataat aatgtcttta tcattatttt tttccatcaa tggtttgctt ttctttgact
     3841 ttttgtggac atttcttttt gcaaataatt caaaacattt ttcaatataa ggttcaatat
     3901 cagtgtattg ccatttctca tccttctttg aaggtatagc t
//
LOCUS       DASCTH010000038         1853 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000056502, whole genome shotgun sequence.
ACCESSION   DASCTH010000038 DASCTH010000000
VERSION     DASCTH010000038.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000056502"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="QGH35_00840"
     CDS             complement(<1..350)
                     /locus_tag="QGH35_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HJL81111.1"
                     /translation="MNLSNLLEEKKIANGLPSLAYTDSEFWQMECETVLANNWTFVGF
                     AHELKKCGDVLPISVAEKPILLVKNTDGNIVAFHNVCKHRCLKLVDQPKNVGKLISCP
                     YHAWAFDLDGKLRAS"
     gene            complement(347..1507)
                     /locus_tag="QGH35_00845"
     CDS             complement(347..1507)
                     /locus_tag="QGH35_00845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008544375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJL81112.1"
                     /translation="MRFGYFANLTNWDHHPYTQVLDETREIAQYCDNTEWDSIWLTEH
                     HLNHEGMDACPNPLMICADLAGRTKRIRLGQAANIITFRNPLRCAEDIAMLDHMSNGR
                     VDVGVGRGVYGREAVHMNVNADLKDQAKNFRLFEETLAIMKKAWTEKTFRHQGEFYKY
                     PNPGFIWKHDMSPPNPDLVDMKTHEMKKIGIVPHPLQLPHPPLWQVVDGHRSIDWAGR
                     NGLNVIMWIPTVKALKERFEVYRKAKSETENRSVPLGEGISLVRDMFVADSMDEARDK
                     AAEGLLTYLRWIAHWRGLGNHLNPGETIPETPGKLDLLTYEFLHPRNLLFGTAEYVSG
                     KIEELRDELNLQHLQVWSNFPCMKQEDVMSSIRKFTEEVMPKFEEKERKIAI"
BASE COUNT          579 a          373 c          296 g          605 t
ORIGIN      
        1 gatgcacgta attttccatc aagatcaaaa gcccaagcgt gataaggaca gctaatcaac
       61 tttccaacat ttttcggttg atcaactaat ttgagacatc gatgtttaca cacattatga
      121 aatgcaacaa tatttccatc tgtattcttt actaatagta taggtttttc agctacagaa
      181 attggtaaaa catcgccaca ttttttgagt tcatgagcaa agccaacaaa agtccaattg
      241 ttagccaata ctgtttcaca ttccatttgc caaaattcac tatctgtata agctagagac
      301 ggcaaaccat tagcaatttt tttttcctct aataaatttg ataagttcat atagctattt
      361 tcctttcctt ttcttcaaat ttaggcatta cctcttcagt aaacttgcgt atgctgctca
      421 ttacatcttc ttgtttcata caaggaaaat ttgaccaaac ctgtaaatgt tgcagattta
      481 attcatcgcg aagttcttcg attttcccac taacatattc agctgtacca aataaaaggt
      541 ttcttggatg aagaaactca taagttagca gatcaagttt tcctggagtt tctggaatgg
      601 tttcaccagg atttaagtga ttacctaaac cacgccaatg agcaatccat cgtaaataag
      661 tgagcaaacc ttcagcagct ttatcacgcg cctcatccat agaatcggcc acaaacatat
      721 cacggaccaa tgagatacct tcaccaagtg gaacagaacg attttcagtt tcagatttag
      781 cttttcgata aacttcaaac cgttccttga gtgctttgac tgtaggtatc cacataataa
      841 catttaatcc atttcgacct gcccaatcaa ttgaacggtg tccatccact acttgccaca
      901 gaggcggatg aggtagttgt aaaggatgtg gcacaatacc aatttttttc atttcatgcg
      961 ttttcatatc tacaagatct ggattaggag gactcatatc atgtttccat ataaaaccag
     1021 gattgggata cttgtaaaat tcgccttgat gtctaaaagt tttttctgtc caagctttct
     1081 tcataatagc taaggtttct tcaaataaac gaaagttttt tgcttgatct tttaaatctg
     1141 catttacatt catatggaca gcttctcgtc cataaacacc tcgaccaaca cctacatcta
     1201 cacgtccatt cgacatatga tctaacattg caatatcctc agcacaacga agtggatttc
     1261 taaatgtaat gatgtttgcg gcttgaccaa gtctgattcg tttggtgcga ccagcaagat
     1321 cagcacatat cataagtggg ttaggacagg cgtccatacc ttcatggttt agatgatgct
     1381 ctgttagcca aattgaatcc cattcagtat tatcacaata ctgagcaatt tctctagttt
     1441 catctaaaac ctgtgtgtaa ggatgatgat cccagtttgt taaatttgca aaataaccaa
     1501 atctcatact ccaaccttct tttcgaataa acaaaaataa agtcggacac ctcttatagc
     1561 cgctgcccga aactcggcct tgtatataga attgattcta acattaatta gcatttaaat
     1621 atttgatcct aatcaatttt ttcaaatttg ataatgatag tattaattta ctacaaagga
     1681 ggtgcgttta tgaaattatc accaccttat acttagttgg tataattaat aaatcgttaa
     1741 ttgagtagtt agtgaaaaaa taaatccatt attatggatt tgcctaagag gcttgaaaag
     1801 gggagctctt atggagcttt ttgctttaag agcgaccccc tttagtagta gac
//
LOCUS       DASCTH010000039         3042 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000056581, whole genome shotgun sequence.
ACCESSION   DASCTH010000039 DASCTH010000000
VERSION     DASCTH010000039.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000056581"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="QGH35_00850"
     CDS             complement(<1..92)
                     /locus_tag="QGH35_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018624420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HJL81113.1"
                     /translation="MLPVIAIVGRSNVGKSTFFNRLTGSKDALVA"
     gene            complement(108..1310)
                     /gene="bamB"
                     /locus_tag="QGH35_00855"
     CDS             complement(108..1310)
                     /gene="bamB"
                     /locus_tag="QGH35_00855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HJL81114.1"
                     /translation="MKPLITRDQNHQRLSAKFVLIISLLIILQGCSLFGRDDEEEELL
                     PTELIEFQGTIDVDERWDVSIGKGHEGLGLALKPTSDGEQIYAASFNGNVAAIELKRG
                     RKVWKNNFDLQFTSGPTYKNGILVLGTNNGELVALDSMTGEMLWTTSVSSEVLAPVAI
                     KDDKIFVRTVDGSLSSLSTADGTQQWIVNHKVPPLSLRGTSTPISFANAVFCGFDDGK
                     VSAYDIEDGTILWDVMLTAPGGRTEIEKVMDIDAPMVVSGNDLYVGSYQGVVGDLALE
                     SGKIVWVVEASVYSGIAADENYVYTSEADGTVRAFSRFTGREAWTKKYMVNRYPTAPV
                     IMGDSIVIGDLEGYVHWLAKATGETQQRLKISSGKISNTPLVINNSIYILTDKGDLIS
                     VEKKIPNS"
     gene            complement(1307..1978)
                     /locus_tag="QGH35_00860"
     CDS             complement(1307..1978)
                     /locus_tag="QGH35_00860"
                     /inference="COORDINATES: protein motif:HMM:NF021492.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJL81115.1"
                     /translation="MDDNLTDQQQAELIKNWLKENAKFLSAVVILGLSGFFGGQYYRE
                     SNQKQAEMASRLFSEMEYSVKLKRISQAQSLLQQIDNDFADTPYQDQGHLLMSKLFID
                     ATEYDNAIIQLEFLLDNSDEESLQHISRLRIARIKLQQNLLDEALITLQIESMGTFEA
                     KYEEIKGDIYSEKGDLLQAGESYRRALDLMLPGNFDYDFIEIKLKDIQNKETLGAVDT
                     EEDTA"
     gene            complement(2008..>3042)
                     /gene="hisS"
                     /locus_tag="QGH35_00865"
     CDS             complement(2008..>3042)
                     /gene="hisS"
                     /locus_tag="QGH35_00865"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HJL81116.1"
                     /translation="SLRPEATAGIVRAGIEAGLFYNQSQKFWSIGPMYRYERPQKGRY
                     RQFHQINIEAYGFPGPDIDAEIILLTTRIWKALNLNSLKLEINSLGTPKSRHIHRKEL
                     QDYFNSHKEDLDADSLRRLDHNPMRILDSKNPDLQDLIESAPKTIDFLDQASSEHFQA
                     LEAILQEHAIEYTLNTRLVRGLDYYTRTVFEWTTDQLGAQATVCGGGRYDELISELGG
                     QATPAIGCAIGLERLIELVDLLDQNQQNDPPKIYIVAVGAQAEGQGLILAEELRDTYS
                     NCSIEMNLGGGSFKQQFKRADKTGADIALIIGEDEIANNTISIKSMQSGEQQKSVKRK
                     DLLDRINQFL"
BASE COUNT          830 a          708 c          532 g          972 t
ORIGIN      
        1 gccacgaggg catcttttga accggttaga cgattaaaaa atgtggattt gccgacattg
       61 gatcggccaa caattgcaat aactggcaac atcgtaactc catcacatca agaatttggg
      121 atttttttct caacagaaat gagatcgccc ttatcggtta aaatgtaaat tgaattattg
      181 attactagtg gagtattgct aatcttacct gagctgattt ttagcctttg ctgggtctca
      241 ccagtggctt tagccaacca atgaacataa ccttccaaat cacctataac aattgaatca
      301 cccataataa ccggtgctgt tggataccta ttaaccatgt atttcttggt ccatgcctcg
      361 cgcccagtaa atctcgaaaa agctctgact gttccgtcgg cctctgaggt atagacataa
      421 ttttcatctg cagcaattcc agaataaacc gatgcttcaa cgacccaaac aatctttccc
      481 gattcaagcg ccaaatcacc cacgacaccc tgataactac cgacataaag atcattgcct
      541 gaaacaacca taggggcatc gatatccatg actttttcaa tctcagttct gccgcccgga
      601 gctgtcaaca tgacatccca gaggattgtt ccatcttcga tatcgtatgc acttactttg
      661 ccatcatcaa aaccacagaa aactgcattc gcgaaactga ttggagtaga cgtacctctg
      721 agactcaatg gcgggacctt atggttaaca atccattgtt gtgtcccatc tgctgttgag
      781 agagagctta ggctgccatc gaccgtgcgg acaaatattt tgtcatcttt aatggctacg
      841 ggtgctaaaa cttcactaga taccgacgtc gtccacaaca tctctccggt cattgaatcc
      901 aaagcaacca attctccatt gttggttcct aacaccagaa taccgttttt ataagtgggt
      961 cctgatgtga attgtaaatc aaaattattt ttccagactt ttcttcctct tttcaattca
     1021 attgctgcaa cattcccatt gaaactagcc gcataaatct gctctccatc agatgttggt
     1081 tttagagcta aacctaatcc ttcatggcct ttgccaatag acacatccca tctctcatca
     1141 acatctatag tgccctgaaa ttcaatcaac tctgttggta acaattcttc ctcttcatca
     1201 tcacgaccaa acaaagagca tccttggaga atgattaaca aagagattat cagcacaaac
     1261 ttagcgctca atcgctgatg gttttgatct ctagttatga gtggtttcat gctgtgtctt
     1321 cctcagtgtc aactgcaccc agggtttcct tgttttgaat atccttgagt ttaatttcaa
     1381 tgaaatcata atcaaaatta ccaggaagca ttaagtctag tgccctacga tatgattctc
     1441 ctgcttgaag tagatcgcct ttctctgagt aaatatctcc tttgatctcc tcatatttag
     1501 cttcaaaggt tcccattgat tctatttgga gtgttattaa tgcttcatct agtaaatttt
     1561 gttgaagttt aattctggca atacgcaatc tcgagatatg ctgcaacgat tcttcatcag
     1621 aattatcaag caagaactcc aattggatga tggcgttgtc atattctgtc gcatcaataa
     1681 aaagcttaga catcagaaga tgaccttgat cttgataagg tgtatctgca aaatcattat
     1741 ctatctgttg gagcaatgac tgagcttgtg aaatacgttt cagcttgact gagtattcca
     1801 tttctgaaaa cagtctagaa gccatttccg cttgtttttg gttgctctct ctatagtact
     1861 gaccaccaaa gaaccctgac aaacccaaaa tcacaacggc actcaaaaat ttggcattct
     1921 cttttagcca atttttgatc agttctgctt gttgttgatc tgttaagttg tcatccatta
     1981 ttatctctcg accgataagg aatttatcta taggaattgg ttgatcctat ccaataaatc
     2041 ttttctttta accgattttt gttgctcccc tgattgcatt gatttgatac tgatggtgtt
     2101 attagcaatc tcatcctcac caatgatcaa agcgatatct gctccggtct tatctgccct
     2161 tttaaattgt tgtttaaaac tacccccacc taaattcatc tcaattgagc aattcgagta
     2221 agtatctctc aactcttctg cgagtatcaa tccctgccct tctgcctgtg cacccacagc
     2281 cacaatataa atttttggag gatcattttg ctggttttga tccaatagat caactaattc
     2341 aatcaaacgc tcaagcccaa tggcacaacc aattgctggt gttgcttgac cgcccaactc
     2401 tgagatcagt tcgtcatatc gaccgcctcc gcagacggtc gcctgagcac ccaattggtc
     2461 cgtcgtccac tcaaaaacag ttctggtata gtaatccaat cccctaacta gacgagtatt
     2521 caaggtatat tcaatggcat gctcttgcaa tatagcctct agagcttgga agtgttcgct
     2581 cgatgcttga tctagaaaat caattgtttt gggtgccgac tcaatgaggt cttgcaagtc
     2641 agggtttttg ctgtctaaaa ttctcattgg attatgatcc agtcttcgca ggctgtctgc
     2701 gtccagatct tccttatggc tgttaaaata atcctgtaat tcttttctat ggatgtgtct
     2761 tgatttcggc gtgcccaaag aattaatctc tagtttcaat gaattcaagt ttagagcttt
     2821 ccatatcctt gttgtcaata aaatgatttc tgcatcaata tcaggtcctg gaaatccata
     2881 agcttctata ttgatctggt gaaattgacg gtatcgccct ttttgcggtc tttcatacct
     2941 atacatcggt cctatagacc agaatttttg gctttgattg taaaataatc ctgcttcaat
     3001 gcctgctctc acgatgccag cagttgcctc aggcctcagt ga
//
LOCUS       DASCTH010000040         2352 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000057588, whole genome shotgun sequence.
ACCESSION   DASCTH010000040 DASCTH010000000
VERSION     DASCTH010000040.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000057588"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            215..>2352
                     /locus_tag="QGH35_00870"
     CDS             215..>2352
                     /locus_tag="QGH35_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81117.1"
                     /translation="MSGLTNAQRVTSVAAAVSAALAGVSTAQGQALEEIIVTATKKAQ
                     SLQSIAGTVQAIAETDLKKAQVVNMEDYAKLIPSMSYINYTPGTGKVYFRGIADDNGT
                     FISEESTAIYLDEQPVTQSGMAPDIRIVDINRIEALSGPQGSLYGSSAQSGTIRIIPN
                     KPDTSEFAASVDVTMRMSAVNPRNEDSWDVSGVVNVPISDNFAIRVVGFTAEDGGFVD
                     NIEGLSPRFGLNKNTDINPGAVREDVNTFKTTGGRVYAQWDLEDGSGSILAGAAFQNN
                     ASDGYSNFDPTVGDLQKIAFYDEPRDDDWTQLSLTVEKDLGWAKLISATSYFDRDIFY
                     QQDRTTYSSYFGSFCYGYYAYVSRYCFQPAGVHYSYNDVPGWQDLFQWNSAKTQEFRL
                     SNQSDDLDWVFGLFYQEREEGWDFHTMTIDYRNSQGFANRVAYIAYALPDRLPVAPTD
                     AWWSSYDRTTWETMAVFGEVNYRFNDNWELTAGGRWFDREADKTYWVENPGGALTADG
                     YLPKNKRDNPSNSDFVPKVSIKYNHSDDLMFYGLYSEGYRPGGVNRGRADNPFYNQGK
                     DTYEPDFLENIEVGLKSTLMDGRFRLNITYFSMDWTDYQIELVDPSNIPCSWPTSPPA
                     PMCGEPWQKVVTNLGDATSDGVILEVLALANDSTEVGVNAQFVKAETATDLTNPDGSV
                     DAPKGSRLPQTPEFKSNLWIEKTMPVSIFGA"
BASE COUNT          711 a          458 c          524 g          659 t
ORIGIN      
        1 actaagaaca gtatcgtaat cgctaaattt ctttaattta catgaattta atcctttgta
       61 atataatatc gttgttataa tattcagtga ttacaagctg ttagatattt gttggaagat
      121 agttctatta tatgggtaat aacctttgag cagccattaa ttgtttatta gtttgaaaat
      181 ttaaattttt ttatacaacc ggggaaaaaa cgttatgtca ggattaacaa acgcacaaag
      241 agtaacaagt gtcgctgctg ctgtctccgc tgcattagcg ggagtttcaa cagctcaggg
      301 acaagcctta gaagaaatta ttgttactgc aacgaaaaaa gcccaaagcc ttcaaagcat
      361 tgctggtact gttcaggcga ttgctgaaac tgatcttaaa aaggcacagg tagtcaacat
      421 ggaagattac gccaaactca tacctagcat gagttatatt aattatactc caggcacggg
      481 taaggtttat ttccgtggta ttgcagatga caatggaaca tttatttctg aagagtcaac
      541 cgcaatctac ttagatgagc aacctgtcac tcaatccgga atggcccctg atattcgaat
      601 agttgatatc aacagaatcg aagcactgtc tggaccacag ggaagtttgt atggttcaag
      661 cgcacaatcc ggcacaataa gaattatacc caacaaacct gatactagtg aatttgctgc
      721 aagtgtagac gtcacaatga gaatgtctgc tgttaaccct agaaatgaag atagctggga
      781 tgtcagtggt gtagtcaacg tgcctatttc tgataatttc gcaatcagag tggttgggtt
      841 tacagctgaa gatggtggtt tcgttgacaa tattgagggc ctcagtcctc gctttggttt
      901 gaataaaaat acagatatca atcctggtgc tgttcgagaa gacgtcaata ctttcaaaac
      961 aacaggcgga agagtctatg cacaatggga tctcgaagat ggctctggct ctattcttgc
     1021 tggtgctgcc tttcagaata atgcatcaga cggttattct aactttgacc caacagttgg
     1081 tgatttacag aaaattgcct tttacgatga acccagagac gatgattgga ctcaactatc
     1141 actgaccgtt gagaaagact taggttgggc aaaacttata tccgcaacct cttattttga
     1201 tcgtgatatt ttctaccaac aagatcgaac cacttattcg agttactttg ggagcttctg
     1261 ttacggctac tatgcatatg taagtaggta ttgctttcag ccagctggag tacattactc
     1321 gtataacgat gttccaggat ggcaggactt gtttcaatgg aattcggcta aaacccaaga
     1381 gttcagatta tccaatcaat cagatgactt ggattgggtg tttgggctgt tttatcaaga
     1441 gcgggaagag ggatgggatt ttcacactat gactatagat tacagaaact cccaaggctt
     1501 tgctaacagg gttgcatata ttgcctacgc tcttccagat agacttccag tagcacccac
     1561 agatgcatgg tggtcatcgt atgatcggac gacctgggaa accatggctg tcttcggaga
     1621 ggtgaactat cgctttaatg acaactggga actgactgct ggtggacgct ggtttgaccg
     1681 tgaagcagat aaaacctatt gggtagaaaa tccaggtgga gcattgactg ctgatggata
     1741 cctgcctaaa aacaaacggg ataatccctc taacagtgac tttgttccta aagtatcaat
     1801 caaatacaat cacagcgatg atctgatgtt ctacgggctg tattctgagg gttatcgacc
     1861 cggaggagtg aaccgtggtc gtgcagacaa tccattctat aatcaaggaa aagataccta
     1921 cgagcctgac tttttggaaa atatagaagt aggtctgaaa tccacactga tggacggaag
     1981 atttcgttta aatattacct atttcagtat ggattggaca gattatcaaa ttgaactggt
     2041 cgacccaagt aacataccat gttcctggcc gacatcaccc ccagcaccaa tgtgtggtga
     2101 accttggcaa aaagtggtga ccaacttagg tgatgcaacc tctgatggag tgattctgga
     2161 agtgctggcc ctagccaatg attctactga ggtaggagtt aatgcacaat ttgtaaaagc
     2221 ggaaacagcc actgacctta caaacccaga tggctctgta gacgctccta aaggatcaag
     2281 attgccacaa actccagagt tcaaatccaa tttatggatt gagaagacca tgccagtcag
     2341 tatatttgga gc
//
LOCUS       DASCTH010000041         5669 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000058293, whole genome shotgun sequence.
ACCESSION   DASCTH010000041 DASCTH010000000
VERSION     DASCTH010000041.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000058293"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            270..1232
                     /gene="trxB"
                     /locus_tag="QGH35_00875"
     CDS             270..1232
                     /gene="trxB"
                     /locus_tag="QGH35_00875"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008169196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HJL81118.1"
                     /translation="MRKNKHSRLIILGAGPAGYSAAIYAARANLNPVLITGSEPGGQL
                     MTTTDVDNWPGDIEGLLGPTLMERMQKHAERFETDIIYDHIEKSDLLKNPFKLYGADT
                     KYTSDAVIIATGSTARYLGLDSEEKFKGKGVSACATCDGFFYKNQHVAVIGGGNVAVE
                     ESLYLSNIAKTVTLIHRRDELRAEKTLQMKLFNKAENNGNVTIVWDHILEEVLGDKNG
                     VNQIKISSTINQKQTTLLDISGVFIAIGHDPNTSIFKNQIHLDDSNYIILDNQSSKFS
                     TQTNIDGVFAAGDVSDPTYRQAITSAGSGCMAALDAEKYLDLIE"
     gene            1272..1490
                     /gene="infA"
                     /locus_tag="QGH35_00880"
     CDS             1272..1490
                     /gene="infA"
                     /locus_tag="QGH35_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001509754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HJL81119.1"
                     /translation="MAKEEAIEMSGVVKETLPNTIFRVELENGHMITAHISGKMRKHY
                     IRILTGDNVTVAMTPYDLTKGRIIFRES"
     gene            1591..1866
                     /locus_tag="QGH35_00885"
     CDS             1591..1866
                     /locus_tag="QGH35_00885"
                     /inference="COORDINATES: protein motif:HMM:NF040598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lpp/OprI family alanine-zipper lipoprotein"
                     /protein_id="HJL81120.1"
                     /translation="MKSKKIFKTFITSSVLLLLLTGCYGALQNQIDEMKGDTERAEEA
                     AAQAAQAADEAAEAADEAAVAAVEAAEAAEAEKEKSERIFERDLGKK"
     gene            complement(1903..2838)
                     /locus_tag="QGH35_00890"
     CDS             complement(1903..2838)
                     /locus_tag="QGH35_00890"
                     /inference="COORDINATES: protein motif:HMM:NF015679.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HJL81121.1"
                     /translation="MKYFEKSFLYFTLLFYCLNTTHAASVFDLEENIEIVGQDETIIA
                     VYEDTLIDLARAHNLGFTEIVIANRSVDKWLPGAGTIIRLPKKFIIPNHSFQDGIIIN
                     LPEYRGYYMNNGKLITFPVGIGRMDWTTPLGKSKVDLKLENPSWYPPKSVQQEYKQRG
                     EYLSPVVPPGPQNPLGKLAMRIDIPGGYFIHGTNKPDGVGMQVSHGCIRLFPEDIKLI
                     FPLTKINTPVLIADQPFKIGILGNEILLEIHETLTTDDVRNNFDYIQSMVKEFLSIKG
                     IQSTVDWTRVKAIFDEKTGIPAMIIQFGITESGLD"
     gene            complement(2866..3831)
                     /locus_tag="QGH35_00895"
     CDS             complement(2866..3831)
                     /locus_tag="QGH35_00895"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HJL81122.1"
                     /translation="MFSKIISTGSYLPEKKITNHDLEQMVDTSNEWILSRTGIQQRRI
                     ATKNQTTSDLAYEAARVAINRAKISNEDIDLIIVGTCTPDVATPNVGTIIQEKLGIKN
                     APAFSVEAACSGFIYAMNIADKFVSSGESKCALVVGAETLSRLTDWSDRNTCVLFADG
                     AGAVIVAPSETPGVLFSQLGANGKYGDLLFVAYGVSKKPEISKPNDYFLQMKGNEVFK
                     VAVKTLESTALDAMEKQNMDPNDIDWYIPHQANKRIIAAVASRLDIPIKKVILTLSEH
                     GNTSAASIPLALDHGIHSGKIKSGETILMQSFGAGFTWGTMILQV"
     gene            complement(3845..4834)
                     /gene="plsX"
                     /locus_tag="QGH35_00900"
     CDS             complement(3845..4834)
                     /gene="plsX"
                     /locus_tag="QGH35_00900"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HJL81123.1"
                     /translation="MNDFITLSLDVMSGEKDPEASITAALNLLALREDVKIILVGNQE
                     IIEKQTLGRTIDRLQILHAEEVVLMNDSPVVVLRKKKQSSMRLAIDLVKNDVSQACIS
                     SGNTGALMAISKYVLKTIPTIKRPALMASMPTVKGHTYVLDLGANSSCSPEQLYQFAL
                     MGTVISREIGEIEQPRVGLLNMGVEASKGNQVVKEAAKLMSSGSINFIGYVEGHNLVE
                     DKADVVVCDGFSGNVAIKTMEGSFSLMDKFLMDSFKTSTLNRFAGMLSKNALNKMKEK
                     LDPRRYNGALLLGLNGVVVKSHGNSDPTGIQHALVTAIEEVQKEIVFKLKEAF"
     gene            complement(4845..5048)
                     /gene="rpmF"
                     /locus_tag="QGH35_00905"
     CDS             complement(4845..5048)
                     /gene="rpmF"
                     /locus_tag="QGH35_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HJL81124.1"
                     /translation="MAVQKKRKTKSRRDMRRSQIKARKPSLTSDELTGETHIRHNISP
                     QGFYRGKQVMTIKQKETIEEDQE"
     gene            complement(5079..5627)
                     /locus_tag="QGH35_00910"
     CDS             complement(5079..5627)
                     /locus_tag="QGH35_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014657.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HJL81125.1"
                     /translation="MFKKNLKLKDFSDFASKNYVLNLTIPAVEFSRLSNLILNDGDLE
                     ANHVSINLEFLTDQSNTQKLKGKIFSLLMLRCSRCLGPVGWQKETLIDLSIADHSSPV
                     KTKQSAINSIIVDYNGVSIEKIVEDEILSMIPMSLMHKTVALCENNDTLSMFLAASDE
                     SNDDQKKNKPFAGLSKLLKNKN"
BASE COUNT         1799 a         1049 c          990 g         1831 t
ORIGIN      
        1 tactaaaacc tggatcatct gaatgatagg tgatcagtga aatcaataaa ataagtgcaa
       61 aagaaaataa ggcaatgatg atggattcac gtaaaatgga gcccttatta aacaattttt
      121 tgacattaat attaaaaata attgcttccc ttttatatag attttttaat aggtaaaact
      181 aatataataa tggaattata tactcatcat cgatactcat ttgcaatatt atcattgttt
      241 atagaaaatc taataaaaca cttttagtca tgagaaaaaa taaacacagt cgtttaatca
      301 tattaggtgc gggtccagct ggttattcgg ctgcaattta cgcagcaaga gccaacttaa
      361 atcctgttct aattacagga tcggaaccag gtggtcaact aatgacaacg actgatgttg
      421 ataattggcc tggtgatatt gaaggattat taggaccaac actaatggaa agaatgcaaa
      481 aacatgcaga gcgttttgaa accgatatta tttatgatca tattgaaaaa tcagacttat
      541 taaaaaatcc ttttaagctt tatggtgcag atactaaata cacaagcgat gcggtcatta
      601 tcgccacggg atcaactgca agatacctag ggcttgacag tgaagaaaaa ttcaaaggaa
      661 aaggagtttc tgcttgtgca acctgtgatg gtttttttta taaaaatcaa catgtcgctg
      721 tcatcggtgg gggcaatgtc gctgttgagg aatcgttgta cttatccaat attgctaaaa
      781 cagtgacctt gatccatagg agggatgaat tgagagcaga aaaaacactg caaatgaaac
      841 tttttaataa agctgaaaac aatggcaatg taacaatagt ttgggatcat atcctagaag
      901 aagttttggg tgataaaaac ggtgttaatc agatcaaaat tagcagcaca attaatcaaa
      961 agcaaacaac attactcgat atttctgggg tttttattgc aataggccat gatccaaata
     1021 catctatttt taagaatcaa attcatttgg atgactccaa ttatattatc ttagataatc
     1081 aatcctcaaa gttcagtaca caaactaaca tcgatggggt ttttgctgca ggggatgtat
     1141 ccgatcccac atataggcaa gcaataactt ctgcaggaag tggttgtatg gctgcgcttg
     1201 atgcagagaa atatctggat ttaattgaat aatacagatt aaaataatac tttcttaaag
     1261 agaatattga tatggcaaaa gaagaagcga tagaaatgag tggggtagta aaagaaacac
     1321 ttcctaatac tatctttaga gttgaactag aaaatggtca tatgattact gcacatatat
     1381 ccggcaaaat gaggaaacat tatatcagaa tcttaaccgg tgataacgtg acagtagcaa
     1441 tgactccata tgacttaacc aagggtagaa taatcttcag agaaagctaa aatcagcttg
     1501 aatcatatta ctctttactg taatttagtg gcaaattctt aagcatagaa ttacaccatc
     1561 tatatggaca tataattgga gaaaaattta atgaaatcca aaaaaatatt taaaactttt
     1621 attacatcaa gtgttttatt attactctta accggttgtt acggtgcatt acagaatcaa
     1681 attgatgaaa tgaagggtga cactgagaga gcagaagaag ctgctgcaca agctgcacaa
     1741 gctgctgacg aagctgctga agctgctgac gaagctgctg tagctgctgt tgaagctgcg
     1801 gaagctgcgg aagctgaaaa agaaaaatct gaaagaatat ttgaaagaga cttgggtaaa
     1861 aaataaccat atttaaagcg ctttcctttt cgaataaaca atctaatcta accctgattc
     1921 agttatacca aactgtataa tcatagctgg gatacctgtt ttctcgtcaa atattgcctt
     1981 aactctagtc caatcaactg tggactgtat gcctttgata gacagaaatt ccttaaccat
     2041 tgactggata taatcgaaat tatttcgcac atcatctgta gtaagagttt cgtgaatttc
     2101 caataaaatt tcattaccta gtattcctat cttaaatggt tggtcagcaa ttaatactgg
     2161 agtattgatc ttagttaagg gaaaaattag ttttatatcc tctgggaaaa gacgtataca
     2221 accatgagaa acttgcattc ctacaccatc tggtttattg gttccatgga taaaataccc
     2281 tccgggtata tcaatacgca ttgccaattt tcccaagggg ttttgaggcc cgggaggaac
     2341 tacaggtgaa aggtattctc ctcgctgctt gtattcttgt tgcacagatt ttggtggata
     2401 ccaactagga ttttccaatt ttaaatcgac cttagacttt cccaaaggag tggtccagtc
     2461 catcctccct atcccaactg ggaaagtaat taatttccca ttattcatat aataccccct
     2521 atattcaggg agattaatga taatcccatc ttggaaacta tgattgggaa tgatgaattt
     2581 cttaggcaaa cgaattatgg ttcccgctcc aggtaaccac ttatcaacag atctattggc
     2641 gattacaatt tcagtaaatc ctagattatg agctctcgcc agatcaatca aagtgtcctc
     2701 ataaacagct ataatggtct cgtcctgacc cacaatttcg atattttctt cgagatcaaa
     2761 aacggaggct gcatgtgtgg tatttaaaca ataaaataga agagtaaaat ataagaaaga
     2821 tttttcaaaa tatttcataa gttctgttta gtttgtgaga actgattaaa cttgaaggat
     2881 cattgtaccc caagtaaatc ccgcaccaaa actctgcatt agaattgttt ctccagattt
     2941 aatctttcct gagtgaatac catgatcaag agccagtgga atagatgctg cagaagtgtt
     3001 cccgtgttct gatagagtta aaatgacttt ctttattggt atatccagtc tactagctac
     3061 agctgcaata attcttttat ttgcttgatg gggtatatac caatcaatat cattaggatc
     3121 catgttttgt ttttccattg catccagtgc agtagactca agggttttaa ctgctacttt
     3181 aaatacttca ttacctttca tttgtaaaaa ataatcattt ggttttgata tttcaggttt
     3241 ttttgaaaca ccgtatgcca caaataatag gtccccatat ttaccattgg ctcccaattg
     3301 agaaaataaa actccaggtg tttctgaagg tgcaacaatt actgcccctg ctccatcagc
     3361 aaataaaacg caagtatttc tatcactcca atcggttaat cgagaaagag tttctgctcc
     3421 taccactaaa gcacacttag attcccctga ggatacgaat ttatctgcaa tattcattgc
     3481 ataaataaat ccactacaag cagcttccac actaaaagca ggtgcgttct taattcctag
     3541 tttttcttga ataattgtac caacattggg agtagcaaca tcaggagtac aggttcctac
     3601 aattattaaa tcaatgtctt cattagaaat cttagcccta tttattgcga ctctagctgc
     3661 ttcgtaagcc aaatctgagg tggtttgatt ctttgtagcg atccgccttt gctgaatacc
     3721 ggttcttgat agaatccatt cgtttgatgt atctaccatt tgctcaagat catgattagt
     3781 gatctttttt tcagggaggt aactaccagt tgatatgatt tttgaaaaca taagaaactc
     3841 tagtttagaa agcttctttc aacttaaaaa cgatttcttt ttggacttct tcaattgcag
     3901 tcaccaatgc atgctgtata ccagtggggt cagaattacc atggctttta acaaccacac
     3961 catttagtcc caataagagt gcaccgttgt atctcctagg atctagtttt tctttcattt
     4021 tatttagtgc attctttgag agcatgccag caaatctatt aagtgtggaa gtcttaaatg
     4081 aatccattag aaatttatcc atcaagctaa aacttccttc catggttttg attgcgacat
     4141 ttccactaaa tccgtcacaa actacaacat cagctttatc ttcgactaaa ttatggcctt
     4201 caacataacc aataaagttg attgagccgg agctcattaa ctttgctgct tcttttacta
     4261 cttgattacc tttactcgct tccacaccca tattcaatag acctacccga ggttgttcaa
     4321 tctctccaat ctctctggag atcactgtgc ccattaaagc aaattggtaa agctgttctg
     4381 gtgaacaact agagttggct cccaaatcaa gaacgtaagt gtgaccctta actgtaggca
     4441 tagaagccat taaggcaggt cttttaattg ttggtatggt ttttagtaca tatttagaaa
     4501 ttgccatcag ggctccagtg tttccggagc taatacatgc ttgagataca tcattcttaa
     4561 caagatcaat cgcaagtctc atggatgatt gctttttctt tcgtaacacg acaaccggcg
     4621 aatcattcat taggacgacc tcttccgcat gcaggatttg aaggcgatct attgttctcc
     4681 ctaaagtttg tttttctatg atttcttggt tgcctacaag aatgattttc acatcctctc
     4741 tcagagccaa tagatttagt gcagcagtaa ttgaagcttc cggatctttc tccccactca
     4801 tcacgtcgag tgatagtgtt ataaaatcat tcaaaacaaa ccctctattc ttgatcttcc
     4861 tctattgttt ctttctgttt aatggtcata acctgtttac cacggtaaaa accttgtggg
     4921 ctaatattgt gcctaatatg ggtctcccct gtgagttcgt cactggttag tgaaggtttc
     4981 ctagctttaa tctgagacct tctcatatct cgtctagatt ttgtttttcg ctttttctgt
     5041 accgccatgc ctaattcctt aaacctgttt agataaaatt agttcttatt ttttaataat
     5101 ttagataacc ctgcaaatgg tttatttttc ttttgatcgt catttgattc atcactcgca
     5161 gctaaaaaca tgcttaaggt atcgttattt tcacataatg caactgtttt atgcatcaag
     5221 gacatcggaa tcattgacaa aatctcatct tcgacaattt tctcaatgga tactccatta
     5281 taatccacaa taatactatt gatggccgat tgttttgttt tgactggact agagtgatct
     5341 gcaatggata aatcaatcaa tgtttctttt tgccacccta cggggccaag acatcgagaa
     5401 catcgaagca tgagcagtga gaaaatttta cctttgagtt tctgagtatt gctttgatct
     5461 gtcaaaaatt ctaaatttat tgatacgtgg tttgcttcta aatctccatc gttcaatatt
     5521 aagttcgata atcttgaaaa ttcaactgct gggattgtaa gattcaaaac ataattcttt
     5581 gatgcaaaat cggaaaagtc tttgagcttt aaattcttct taaacatgtt tctagtctgg
     5641 gtatactgtc attctaatcg actaaaaaa
//
LOCUS       DASCTH010000042         2159 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000060032, whole genome shotgun sequence.
ACCESSION   DASCTH010000042 DASCTH010000000
VERSION     DASCTH010000042.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000060032"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="QGH35_00915"
     CDS             complement(<1..167)
                     /locus_tag="QGH35_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HJL81126.1"
                     /translation="MIIQHNIRKRFGQNFLVDNNIINKILDLIEPRKHDHFIEIGPGM
                     GALTFPILDRI"
     gene            complement(170..1165)
                     /gene="pdxA"
                     /locus_tag="QGH35_00920"
     CDS             complement(170..1165)
                     /gene="pdxA"
                     /locus_tag="QGH35_00920"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HJL81127.1"
                     /translation="MTNTDKPIPFIVTSGEPSGIGPDIVLSLAMTTKSEQFVVFGNID
                     MLKSRAEVLGMNINIVPYKVGSELNHTESNSLLVKDFELSETVIPGQLNKENSVYVLE
                     MIKEATLGCLSGQYKGLITAPVHKHIINENGIEFSGHTEYIAELCNSKNPVMTFISDT
                     MQMAFATTHVPLKKISRLITTDLLVDICRILHVDLERLFDLKSPKIGILGLNPHAGEN
                     GILGDEEINTILPAIKIIQNEGINAIGPLAADTAFLFNQSQRVDLMLVMYHDQGLPLF
                     KFSNHLQSANVTLGIPIIRTSVNHGVALDKAASGEALNDSLHYAITIAENMYGHA"
     gene            complement(1176..>2159)
                     /locus_tag="QGH35_00925"
     CDS             complement(1176..>2159)
                     /locus_tag="QGH35_00925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJL81128.1"
                     /translation="NNVAKNNNTTFAEFPQLLADEGVNYEDYRQELREQITIEQLRQR
                     DVVSRIQISPSELESFLVLQKNRDALNFDYDLSHILIPVSASASDSQITSAKILSTKI
                     YERIVKGEKFETLAVEYSQGQQALSGGKLGWLKGEQLPTSFIEAASQLEKGETCQPFR
                     SSSGFHLILLNDFKGQEPILEEQIHVRHILIKTNEILDDDAAKEKLKTIRDQITKDGD
                     FGAIAAATSEDTGSAQEGGDMGWTGLGFFVPEFEAVVNSLVENEISEPFKSRYGWHIV
                     QLLGNRIYDNTEDIGRNKAIESIRNSKLSDEIELWARQLRDEAFVEYLSYD"
BASE COUNT          627 a          402 c          377 g          753 t
ORIGIN      
        1 tttattcgat caagaattgg aaaggttaat gcacccatac caggaccaat ttcaataaaa
       61 tggtcatgct tcctaggctc aatcaagtca agaatcttat taattatatt attatcaact
      121 aggaaatttt gaccaaagcg tttgcgaata ttgtgttgta ttatcactct tatgcgtgtc
      181 catacatatt ttcagcaatt gtaattgcat agtgtaagct gtcattcaat gcttctccac
      241 ttgcagcttt atccaaagca acaccatgat ttactgaggt tctaataatt ggtatgccta
      301 gcgtcacatt ggcagattgt aggtgattag aaaatttaaa tagtggcaaa ccttgatcgt
      361 ggtacataac aagcatcagg tcaactcttt gtgattggtt aaacaaaaaa gctgtgtcag
      421 cagccaatgg tcctattgcg ttaatgcctt cattttgtat tatttttatg gccggcaata
      481 tagtgttaat ttcttcatca cccaatatgc cattctctcc tgcatgtggg ttgaggccta
      541 aaataccgat ttttggagat tttaggtcaa acaaacgctc taaatccacg tgtaaaattc
      601 tgcaaatatc aactaataga tcagttgtta ttagtcttga gatttttttt agtggcacat
      661 gagttgttgc aaaagccatt tgcatggtat cggatataaa agtcattact ggatttttgc
      721 tgttgcacaa ttcggcaata tattcagtgt gaccactaaa ctcaatccca ttttcattaa
      781 ttatatgttt atgaacgggt gctgtaatga gacctttgta ttgaccagat aagcacccta
      841 atgttgcttc tttaatcatc tcaaggacat agactgagtt ttccttattg agttgaccag
      901 ggatgactgt ttctgaaagt tcaaaatctt taaccaagag ggagttagat tctgtatggt
      961 ttaactctga gccaacttta taaggtacta tattaatgtt catgccaaga acttcggctc
     1021 ttgatttaag catatcaatg ttgccaaaaa ccacaaattg ttcagatttt gttgtcatgg
     1081 ctagagataa aactatatct ggcccaatgc ctgagggttc gcctgaggtt acaatgaatg
     1141 gaataggttt gtctgtgttg gtcacaaggg gaaaactaat cgtaggataa atattctaca
     1201 aatgcttcat ccctaagttg acgtgcccat aattcaattt catcagagag tttagaattt
     1261 ctaattgatt caatggcctt atttcttcct atatcttcag tgttatcata aattctatta
     1321 cctaataatt gaacaatatg ccagccatat ctagatttaa aaggttcact gatttcattt
     1381 tctactaaag aattcacaac tgcttcaaat tctggaacaa agaacccaag accagtccat
     1441 cccatatctc ctccttcttg tgcagagcca gtgtcttcag aggttgccgc agctattgcg
     1501 ccgaaatctc catctttggt aatttgatct ctgattgttt tcagtttttc tttagcagca
     1561 tcgtcatcta gaatctcatt ggtttttatc aaaatatgtc tgacatgaat ttgctcctct
     1621 aatatgggct cttgtccttt gaaatcattt aatagtatca agtgaaaacc actagaactc
     1681 ctaaagggtt gacatgtttc tcccttttct aactgtgatg cagcctcgat aaaacttgta
     1741 ggtaattgtt caccttttaa ccaacctaat tttccaccac taagagcttg ttgtccctga
     1801 gaatattcca ctgctagggt ttcaaatttt tctcctttga caattcgttc atagattttt
     1861 gttgataaaa ttttggcact tgtaatttga ctgtctgatg ccgaagcaga gacaggaata
     1921 agaatatgcg aaaggtcata atcaaaatta agagcatcac ggtttttttg cagtactaaa
     1981 aagctttcaa gttcgcttgg cgaaatttga attcttgaga caacatctct ttgtcgcagt
     2041 tgttctatgg ttatttgttc tctcagttct tgacgataat cttcataatt aacaccttca
     2101 tccgctagta actgaggaaa ttctgcaaag gttgtattgt tatttttggc aacattatt
//
LOCUS       DASCTH010000043         1531 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000062560, whole genome shotgun sequence.
ACCESSION   DASCTH010000043 DASCTH010000000
VERSION     DASCTH010000043.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000062560"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="QGH35_00930"
     CDS             <1..432
                     /locus_tag="QGH35_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81129.1"
                     /translation="GILGLEGSYGVSKFAALSLSEGLFQSLKKINSKIGASALCPGFV
                     ATNLTDSQRNRPEHLASEKKSNFLLKQLAASVLKRGKKPDEIAARVIEAIKTNSFYIL
                     PHPIYDEMVKERYERILARTEPETVDIKMAWLGSLLRREEY"
     gene            448..>1531
                     /locus_tag="QGH35_00935"
     CDS             448..>1531
                     /locus_tag="QGH35_00935"
                     /inference="COORDINATES: protein motif:HMM:NF020039.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HJL81130.1"
                     /translation="MKNFIYLTLSLICIICISLLILFSTQRSDIEVIAITDCKSDSKL
                     TVYCGFSNPEDMVVLPDKKNLLVSEFGALPPMNPENLPGQISLFNTDTLEIKPINISL
                     GDKEWGHANCTRSDSIFSPHGIDLIQRNDGRYQLAVVNHMPRETVEMFELTDVQNTWS
                     LIWKGCIDAPELGYFNDVALKKDGTFYVSHMYNRDKSLLWLVYATYVTIDTGFVYQWD
                     ADRGYQKVSGTEGSYPNGVELSLDENYLFTNYILNRKTSKLDLASGTVIQEHFGVGMP
                     DNLTIDGEFIWVATQDHTGTDLLLHCGSDDILQCSLPFTIFKLKQSDLSEVAVFSFSN
                     TVMGSVTVAVPDEDRVWLGTFHGDRMA"
BASE COUNT          477 a          279 c          318 g          457 t
ORIGIN      
        1 ggtatcctgg gccttgaagg tagttatggg gtgagcaagt tcgctgcttt gtcactcagc
       61 gagggactgt ttcaaagttt aaaaaagatc aatagtaaga ttggagcatc cgccctgtgt
      121 cctggttttg tagctacaaa ccttactgat tctcaacgga atcgaccaga acatttagca
      181 tccgagaaaa aatccaattt cttgttaaaa caactcgccg catcagtatt aaaaagagga
      241 aaaaaaccag atgaaatagc ggcaagagtt atcgaggcta ttaaaaccaa tagtttttac
      301 atcctgcctc atccgatcta cgatgaaatg gttaaagaga gatatgaaag aattcttgca
      361 agaactgaac cagaaacagt tgacataaaa atggcatggt tgggttcgct gttacgtaga
      421 gaggaatatt gattaacaat gaaggtagtg aaaaatttta tctacctcac attgtcactg
      481 atctgcataa tttgtatatc gctgctgatt cttttttcca cccaacgttc agatatagaa
      541 gtcatcgcaa taaccgattg taagtcggac tcaaaattaa ctgtatattg tggcttttct
      601 aacccagaag atatggtggt tttaccagat aaaaaaaact tgttggtctc agagtttggc
      661 gctcttcctc ccatgaatcc cgaaaacctc ccaggtcaaa tctcactatt taataccgat
      721 acattggaga taaaaccgat aaatatttca ctaggtgaca aagaatgggg acatgcaaac
      781 tgcacccgta gtgattcaat tttctctcct catggtattg atcttattca aaggaacgat
      841 ggtcgttatc aattagcggt agttaaccat atgcccagag aaactgttga gatgtttgaa
      901 ttaactgatg ttcaaaatac ttggtcactg atttggaaag gttgtattga tgcacctgaa
      961 ttaggctact tcaacgatgt tgctctaaag aaagacggta ctttttatgt atcgcatatg
     1021 tataacagag acaaatcctt attatggttg gtctatgcta catatgtcac cattgataca
     1081 ggttttgtct accaatggga tgcagatcgc ggataccaaa aagtttcagg caccgaaggg
     1141 tcctatccca atggggttga actttctctc gatgagaact atttatttac caattatatt
     1201 ttaaatcgaa aaacttctaa gttagatctt gccagtggaa ctgtaatcca ggaacacttt
     1261 ggagtcggaa tgccagataa tttaactata gatggagaat ttatttgggt tgctacccaa
     1321 gatcacacgg gcacagatct gttgttgcat tgtggtagtg atgatattct ccaatgctca
     1381 ttaccgttta caatctttaa gctaaaacaa agtgacttaa gcgaagttgc agtgttttca
     1441 ttttctaaca ccgtaatggg ttcggtgacg gtagcagttc cagatgagga tagagtatgg
     1501 cttggtacct ttcatggcga tagaatggca a
//
LOCUS       DASCTH010000044         7964 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000065447, whole genome shotgun sequence.
ACCESSION   DASCTH010000044 DASCTH010000000
VERSION     DASCTH010000044.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7964)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7964)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7964
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000065447"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /gene="prfB"
                     /locus_tag="QGH35_00940"
     CDS             <1..133
                     /gene="prfB"
                     /locus_tag="QGH35_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155788451.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HJL81131.1"
                     /translation="IRSYVLDQSRVKDLRTQIESSNPEAILDGDINKFLEASLKQNL"
     gene            152..1645
                     /gene="lysS"
                     /locus_tag="QGH35_00945"
     CDS             152..1645
                     /gene="lysS"
                     /locus_tag="QGH35_00945"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HJL81132.1"
                     /translation="MSEKNQDQNKLILERRKKLTLLRDQGFNFPKSIEIDAYAHELTE
                     AFGDLSNDELGQSKKEVRIAGRMMAKRVMGKSSFSKLKDISGDIQIFLSSSDLSKETY
                     DEFKTLDVGDIIWVKGLLFKTKTDELTIKVLELELLTKSLRPLPEKYHGLTDTETRYR
                     RRYLDLIMSDQTRDVFKTRSQIVSIIRGFLDNRNIMEVETPMMQPIPGGAIAKPFVTH
                     HNTLGKDFYLRIAPELYLKRLVVGGLDRVYEINRSFRNEGVSTQHNPEFTMLELYIAH
                     ESPEYLMTLIEEMIAEICMEINGSKMIQYQERVYDLSGSYKRMTLEESVIHFNPNFDA
                     TKIRDRDYLLNTANELGIKISDDALAGKILLEIFEKTVEENLIEPIFITGYPRDVSPL
                     SRANDEDPWLVDRFEFFLAGRELANGFQEINDPDDQSERFKLQVQERDSGDDEAMVYD
                     EDYIQALEYGLPPTAGLGVGVDRLIMFFTDSPSIRDVLFFPHMREKI"
     gene            complement(1681..2511)
                     /locus_tag="QGH35_00950"
     CDS             complement(1681..2511)
                     /locus_tag="QGH35_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013720.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81133.1"
                     /translation="MKHLDHFSIISISGADSTEFIQGQMTQDISAIGDDEARMTAILN
                     PQGRVMSTALIMHWDDSIILVLNKDTVDDLIVWLSRFILRSKVTVSRLEAHIYGLNQN
                     LANIESDSLDLEKEIFCLRSIETDAERTLLITKSLHDFSKSSITTMSSRNWQLADIQA
                     GIPIIYKENIAKFIPQMVNLDLINGISFNKGCYTGQEIVARVQHRGKIKRRMFHISTQ
                     QSNTEIRPGTPVLLGDSEVGTIIQSVQYKNQIHSLAVIKNDASKKKLLVNGSAIKIQS
                     "
     gene            2557..3000
                     /gene="sdhC"
                     /locus_tag="QGH35_00955"
     CDS             2557..3000
                     /gene="sdhC"
                     /locus_tag="QGH35_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HJL81134.1"
                     /translation="MFEEINLSIIDAIKTYQNMNKNKQPLSPHLSIYRWQVTNTLSIF
                     HRLSGLLLFLAALDFAVWLGSIALGSGAYSYVKGIYSSNFLLLIWLLVSLSFFYHLFN
                     GMRHLFWDLGKGFEESQVKWTGTLVILFSIMITGLFWYLLLGKFG"
     gene            3004..3396
                     /gene="sdhD"
                     /locus_tag="QGH35_00960"
     CDS             3004..3396
                     /gene="sdhD"
                     /locus_tag="QGH35_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HJL81135.1"
                     /translation="MKIIKGILKLLCLASFEEAKEHWRSQRETAVIVMVLGFWLIYQL
                     LFVLPSFEYNEVLFWAAEPLNALILLLLSLFLIYHAELGVQVVIEDYISTENVRKYVL
                     LLLRLLRISVVIVSIVSISSILIGGGSS"
     gene            3393..5180
                     /gene="sdhA"
                     /locus_tag="QGH35_00965"
     CDS             3393..5180
                     /gene="sdhA"
                     /locus_tag="QGH35_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HJL81136.1"
                     /translation="MSDKYELVEHRYDVVVVGAGGAGLRATLGLVEAGLSTACVTKVF
                     PTRSHTVAAQGGISAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIAYMCKQASSAVLE
                     LERYGIPFSRTTDGKIYQRAFGGMTTHFGEGIAQRTCAAADRTGHAMLHTLYQQSIKN
                     KAEFFIEFFALELLMDEGTCKGVLALEMATGKLHLFRAQKVILATGGHGRAYFSCTAA
                     HICTGDGSGMALRAGLPLQDMEFVQFHPTGLYGVGCLITEGVRGEGGYLTNSENERFM
                     ERYAPSAKDLASRDVVSRSITMEILEGRGVGENKDRMNLNLQHLDPQVIEERLPGIAE
                     TAKIFVDVDVTKEPIPIIPTVHYNMGGIPTNIKTEVITLNQDGEEEIVPGLMALGEGA
                     SVSVHGANRLGSNSLLDLVVFGREAAQQCAKTLTPGSTLNDIPQTIVDKTIDKFDKTR
                     FSNGDLSTAEIRNQMQQIMQKHASVFRTEEILKEGADLLSDCLSSFAKVSVSDKGLIW
                     NTDLIETLELENLLGQAMITMQCALHRKESRGAHARDDYPVRDDENWLKHSLGWLSND
                     GVTRIDYHPVKLDTKTDEIESIPLKARVY"
     gene            5194..5976
                     /locus_tag="QGH35_00970"
     CDS             5194..5976
                     /locus_tag="QGH35_00970"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HJL81137.1"
                     /translation="MADFRLPKNSRVKKGRTFLLNSQVNNSRNFVIYRYDPEKDQNPR
                     LDTFEVDLDSCGPMVLDALIKIKTEIDSSLSFRRSCREGICGSCSHNVNGVNTLACTQ
                     SIKEIKGDIRIYPLPHMNVIKDLITDLSVFYDQYASVEPWLKTNTAPSEGKEQYQTKE
                     EQEKIDKPSACILCACCSTSCPSYWWNSDKYLGPAALLASYRWLQDSRDEAKDERLQL
                     LNDSSKLYRCHTIMNCTLACPKGLNPGQAIAEIKKMIATREL"
     gene            5973..6242
                     /locus_tag="QGH35_00975"
     CDS             5973..6242
                     /locus_tag="QGH35_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015870.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HJL81138.1"
                     /translation="MTNNGSIELSKIRWRCRRGLKELDFLLLNYFNQKYTIANTEDKE
                     AFLHLLEFQDPALIEFFADPKRIQDPGIRRIISEILGSNDGYQLK"
     gene            6239..>7964
                     /gene="lepA"
                     /locus_tag="QGH35_00980"
     CDS             6239..>7964
                     /gene="lepA"
                     /locus_tag="QGH35_00980"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HJL81139.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRLIQLCGGLNEREMSDQVLDTM
                     DLEKERGITIKAQSASLKYMPKDQDQYIFNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQSVANSYTAIEQGLEILPVINKIDLPAADPDKAIKDIQEIIGIDITNVSL
                     ISAKTGEGMEELMDQIVSSIPSPEGILEDRLQALIIDSWFDQYVGVISLLRIKNGVIR
                     KGEKITVMSTGYEYTVNSIGVFTPKIENRECLSAGEVGFLIAGIKDIFGAPVGDTITN
                     KTNPSRSALPGFKTIQPKVFAGLYPSVSEDLEVFREALDKLKLNDASLFFEPESSTAL
                     GIGFRCGFLGMLHMDIVQERLEREYNLELVTTSPTVIYEILKTNGAVIKIDNPNSLPE
                     MNHIKEIREPIIEASILVPPDFVGNVIQLCERRRGVQKRLQYLGRQVVLDYELPLAEI
                     VLDFFDQLKSVSKGYASFDYSFLGFQESRLVRLDILVNREKIDAFSVIFHKDMAYHQG
                     RKIIEKLTQLIPRQMFEVALQAVIGSKIIARGNVRAMRKNVTAKCYGGDVTRKKKLLE
                     KQKAGKKRM"
BASE COUNT         2518 a         1336 c         1699 g         2411 t
ORIGIN      
        1 aatcagatct tatgttcttg atcagtctag agttaaagat ttaagaaccc aaattgaatc
       61 cagtaatcca gaagctatac tggatggaga tatcaataaa tttttagaag ccagtcttaa
      121 acaaaatttg taaaaaaaag ttgagataat aatgtctgaa aaaaatcagg atcaaaataa
      181 attaattcta gagagaagaa agaaactgac tttacttaga gatcagggat tcaattttcc
      241 aaaatctata gaaatagatg cctatgctca tgaactgact gaagcttttg gagatttatc
      301 aaacgatgaa ttgggtcaaa gcaagaaaga agttaggatt gcgggtcgga tgatggccaa
      361 aagagtcatg ggcaaatcca gcttttctaa gctcaaagat atctccggtg atattcagat
      421 cttcctcagt tcctctgatc tctcaaaaga aacctacgat gaatttaaaa cactagatgt
      481 tggggatatt atctgggtca aaggactctt gtttaaaaca aagaccgacg aattgaccat
      541 taaagtacta gaattggaac tgttaactaa gtctctgaga cctttgcctg aaaagtatca
      601 cgggttaacg gatacggaaa ccagatatcg tcgcagatat cttgatttga ttatgtcaga
      661 tcaaactaga gatgttttta agacaagatc ccagattgtc tccattatta gaggtttttt
      721 agataataga aatattatgg aagttgagac tccaatgatg cagcccattc cagggggggc
      781 aatagcaaaa ccttttgtga cccatcataa tactttgggt aaagattttt atctcaggat
      841 tgcgcccgag ctgtatttga aaagattggt tgttggtggt ctggacagag tctatgaaat
      901 taatcgttct ttcaggaatg agggagtctc tacacaacat aatccagaat ttaccatgct
      961 agaactctac atagctcatg aatcaccaga gtatcttatg accctaatcg aggagatgat
     1021 agctgagatt tgtatggaaa ttaatggctc aaagatgatc caataccaag agcgagtata
     1081 tgatttatcc ggatcttaca aaagaatgac actggaagag tcagttatac actttaatcc
     1141 taattttgat gctacaaaaa taagggatcg ggactattta ttgaacactg caaatgaatt
     1201 aggaattaaa atcagtgacg atgctctagc tgggaaaata ttgcttgaaa tttttgagaa
     1261 gaccgtcgag gaaaacttaa tagaaccaat atttatcact ggttatccaa gagatgtttc
     1321 tcccctatca agagcaaatg atgaggatcc atggcttgtg gatcgtttcg aattctttct
     1381 cgccggtcgc gaactagcca atggttttca ggaaataaat gatccggacg atcagtcaga
     1441 acgctttaaa ttacaagttc aagagagaga tagtggtgat gatgaagcaa tggtctatga
     1501 tgaagattat attcaagcac tagaatatgg actaccgcca acagcaggat tgggcgttgg
     1561 ggttgatcgg ttgataatgt tttttacaga tagtccttca ataagagatg ttttattttt
     1621 tccacatatg agagaaaaga tataataacc agataaaaag agctctattt aatacactct
     1681 ctaactttgt atctttattg ccgatccatt cacgagcaat ttcttttttg atgcatcatt
     1741 cttgattaca gctaatgaat gaatttgatt cttgtattgt accgactgga taatagtccc
     1801 aacttctgag tctccaagaa gaactggagt gccaggtctg atttcagtat tactctgttg
     1861 agtagaaatg tgaaacattc ttctttttat cttcccccta tgttgaactc tagcgactat
     1921 ttcttgtccg gtgtaacagc ctttattgaa gctaattcca ttgatgaggt ccagattaac
     1981 catttgtgga ataaattttg caatgttttc tttataaatg attgggatcc cagcttgtat
     2041 atcagcgagc tgccaattcc tactagacat tgttgtaatg gaactcttac taaaatcatg
     2101 aagggattta gttattaata gggtcctctc tgcatctgtc tcaatacttc tcaggcagaa
     2161 aatttctttt tcaaggtcaa ggctatcgga ttctatgttt gctagatttt gattaagccc
     2221 gtaaatatga gcttccaatc ttgagacagt tactttagac ctcaggataa accgactcaa
     2281 ccaaacaatg agatcatcga ccgtgtcctt gtttagaact agtatgatgg aatcatccca
     2341 atgcattatt aatgctgtag acatcactct tccttgtggg ttcaatatgg cggtcattct
     2401 cgcttcgtca tcgcctattg cactaatgtc ttgagtcatt tgtccttgga tgaactctgt
     2461 tgaatcagct ccagagattg aaataattga aaaatgatcg agatgtttca ttgacttgtt
     2521 tggatccaga taaatgaatt catagggcaa aaataaatgt ttgaagaaat taatttaagt
     2581 ataattgatg cgataaaaac ataccaaaat atgaacaaga ataaacaacc tttatccccc
     2641 catttgagta tttaccgatg gcaagtaact aatacattgt ccatatttca taggctctct
     2701 ggcttattgt tatttttagc agcactcgat tttgcagtct ggctgggatc gatagcacta
     2761 gggtccggtg cctatagcta tgttaaagga atttattcaa gcaatttctt gttgctgata
     2821 tggttacttg ttagcctgtc atttttttat catttgttta atggcatgcg tcatttattt
     2881 tgggatcttg gtaaagggtt tgaggaatcc caagtcaaat ggacaggaac cttggttatc
     2941 ctgttttcta ttatgattac ggggttattt tggtatctgc tgctgggaaa gtttgggtaa
     3001 gacatgaaaa tcataaaggg gattttaaaa ctattgtgtt tggcatcatt tgaggaggca
     3061 aaagagcatt ggagaagtca acgtgaaact gctgttatag taatggtgtt gggtttttgg
     3121 ttgatttatc aactactttt tgtattacct agttttgaat ataacgaggt tttattttgg
     3181 gctgcagaac ctctcaacgc gctcatcctg ttgttattgt cattattcct catttatcat
     3241 gctgaactag gtgtccaggt tgtgattgaa gattatattt caactgaaaa tgtcaggaaa
     3301 tacgttctac ttttattacg attactgaga atctcggtgg tgatagtgtc tatcgtatct
     3361 atttcatcaa tattaattgg tggtggatca tcatgagtga caagtacgaa ttagtggagc
     3421 atcgttatga tgtcgtggtt gtcggtgcag gaggggctgg cttgagagca acattgggtc
     3481 tggtagaagc aggtctatcc actgcttgtg ttactaaagt ttttcccaca agaagccata
     3541 cggttgcagc tcagggtggt attagtgccg cgcttgggaa catgggggaa gacgattggc
     3601 gatggcacat gtacgatacc gtcaaaggat ctgattggtt aggagatcaa gatgccattg
     3661 cctatatgtg taaacaagca tcttctgcag tacttgaact tgagagatac ggtattcctt
     3721 tctctagaac cactgatgga aaaatttatc aacgagcttt cggtggaatg acaactcatt
     3781 ttggtgaagg tatagcacaa agaacctgtg ctgctgcaga cagaaccggt catgccatgt
     3841 tgcacacgct atatcaacaa tccataaaaa ataaggcaga attctttatt gagttctttg
     3901 ctctagaatt attgatggat gaagggactt gtaaaggtgt tctcgcactt gaaatggcaa
     3961 ccggaaaact tcatcttttt agggcgcaga aagtcatatt agccacagga ggtcatggta
     4021 gggcttactt ttcttgtaca gcagcccata tctgcaccgg agatggttca ggcatggcac
     4081 tcagggcagg attacctctt caagatatgg agtttgtcca atttcatccc acgggtcttt
     4141 atggtgttgg ctgtttaatt accgaaggtg ttagaggtga aggtggttat ttaacaaatt
     4201 ctgagaacga acgatttatg gaaagatatg ctcctagtgc aaaggatcta gcctctaggg
     4261 atgtggttag ccgctcgatt accatggaaa ttcttgaagg aagaggcgtc ggagagaata
     4321 aggacagaat gaatcttaat ctccagcatc tggatcctca agtcattgag gaacgactac
     4381 ccggaattgc tgaaacggca aaaatttttg tcgatgttga tgtaaccaag gaaccgatac
     4441 ccatcattcc tactgttcat tacaacatgg gaggtattcc taccaacatc aaaacagagg
     4501 tgattactct gaatcaagat ggtgaagaag agattgttcc aggattgatg gcgttgggcg
     4561 aaggtgcatc agtttccgtg catggggcta acagattggg gtctaattcc ttacttgact
     4621 tggtggtttt cggtcgagag gcagcccaac aatgtgcaaa gactctcact cctggttcaa
     4681 cactcaatga tatacctcaa acaattgtgg ataaaacaat agacaaattt gataaaacac
     4741 ggttttcaaa tggcgatctt tctacagctg aaatcaggaa tcaaatgcaa caaataatgc
     4801 aaaaacatgc ttccgtattt agaactgaag agatattaaa agaaggagct gatttgttat
     4861 ctgattgttt aagttctttt gctaaggttt cagtttcaga taaaggactg atctggaata
     4921 ctgatttaat tgaaactctc gaacttgaaa atcttctagg acaagcgatg attaccatgc
     4981 aatgtgcatt acatcgaaag gagagcaggg gagcacatgc ccgcgatgat tatcccgtgc
     5041 gtgatgatga gaattggctc aagcatagtc ttggatggct cagtaatgat ggagtaacaa
     5101 gaattgatta tcatccggta aaacttgata ctaaaacaga tgaaattgag tccatacctt
     5161 taaaagcaag ggtttattaa aggaaaagta gcaatggccg actttagatt accgaaaaat
     5221 tcaagagtga agaaaggtag aacattttta ctaaactcac aggtgaataa ttctcgaaac
     5281 tttgttattt atcgttatga tccagaaaaa gatcaaaatc caaggctaga tacttttgag
     5341 gttgatctag actcttgtgg gcctatggtt ttagatgcat taattaagat caagacagag
     5401 attgactcat ctttatcatt ccgacgatct tgcagagaag gcatttgtgg ttcttgttca
     5461 cacaatgtaa atggagtaaa tactctggct tgtacgcaat caattaagga aataaaaggt
     5521 gatatcagaa tctatcccct gccacacatg aatgtcataa aagacttaat cactgatttg
     5581 tcggtttttt atgaccaata tgcttcagtg gaaccgtggc tgaaaacaaa cacagctcct
     5641 tctgaaggta aggaacaata tcagaccaaa gaggaacaag agaagataga taaaccttct
     5701 gcctgcatcc tttgtgcgtg ttgttcaact agttgcccga gttattggtg gaacagtgac
     5761 aaatatttag gtcctgccgc attattagca tcatacagat ggttgcaaga cagtagagac
     5821 gaagccaagg acgaacgatt gcagttactt aatgactcat caaaattata tcgctgtcat
     5881 acaattatga actgcacttt ggcatgtcct aaaggactta atcccggtca agctattgct
     5941 gaaataaaaa agatgatagc aacgagagag ttgtgaccaa caacggctcc atcgaattat
     6001 ctaaaattag atggagatgt agaagaggcc taaaggagtt agattttctt ctcctcaatt
     6061 actttaatca gaaatataca atagccaata cagaggataa agaagcattt ctgcatttgc
     6121 tggaatttca agaccctgct ttgattgaat tttttgcaga tccaaaaaga atccaagatc
     6181 caggaattcg acgaatcatt tctgaaatct taggcagcaa cgatggttac caacttaaat
     6241 gaaaaatata cgaaattttt caattattgc ccatatagac catgggaagt caactctggc
     6301 agatcgctta atccaattat gtggtgggtt gaatgagcga gaaatgagtg atcaggtatt
     6361 ggatacgatg gatttggaga aagaaagagg aataaccatt aaagcacaga gtgcttccct
     6421 aaaatacatg cccaaggatc aagatcaata tatctttaac ctgattgata cccctggcca
     6481 tgttgatttt tcttatgaag tttcacgatc tctggctgct tgtgaagggg cacttttggt
     6541 cgtagatgcg tctcagggtg ttgaggctca aagtgttgcc aacagttata ccgcaattga
     6601 acaagggtta gaaattttac ctgtgatcaa taaaattgat ctgccagctg ctgatccgga
     6661 taaagccatt aaggatattc aagaaattat tggcattgat ataacaaatg tttcattaat
     6721 aagcgctaaa acaggggagg gtatggaaga attgatggat caaattgtct cctcaatacc
     6781 atcccccgaa ggaattctag aagacaggct gcaagctttg attattgatt catggtttga
     6841 tcaatatgtt ggtgttattt cattactgag aataaagaat ggggttataa ggaaaggaga
     6901 gaaaattacc gtcatgtcga ccggttacga atacacagtt aactcaatag gagtatttac
     6961 tcccaagata gagaacaggg agtgcctttc tgctggcgaa gttggatttt taattgctgg
     7021 aattaaagat atttttggag caccagtagg agataccatt actaataaaa ccaatccaag
     7081 ccgatcagct ttacctggtt ttaaaactat ccaaccaaag gtctttgcag gtctatatcc
     7141 ttctgtttcc gaagatctgg aagtctttag agaagccctt gataagctta aattaaacga
     7201 tgcatcatta ttttttgaac ccgaatcctc gactgcttta gggattggtt ttcgatgtgg
     7261 ttttttaggg atgctgcata tggacattgt tcaggaaagg ttagagcggg aatataatct
     7321 tgaattggta acaacatccc caacagtcat ttacgaaatt ttaaaaacta atggtgctgt
     7381 aatcaaaata gacaatccta attctcttcc tgaaatgaat catataaaag aaatccgtga
     7441 accaattatt gaagctagta tattagtgcc tcccgatttt gtaggtaatg ttatacagtt
     7501 gtgtgaaaga agaagagggg tgcagaaacg gcttcaatat ttgggtcgac aagtggtatt
     7561 agattatgaa cttcccttag ctgaaattgt tctagatttc tttgatcagt tgaagtctgt
     7621 atccaaaggc tatgcatcct ttgattacag ttttttagga tttcaagaaa gcagattagt
     7681 gcgattggat attttagtga atagagaaaa aatcgatgct ttctcagtta tttttcataa
     7741 agatatggct tatcatcagg gtcgtaaaat tattgaaaaa ttaactcaat tgattcctag
     7801 acagatgttt gaagttgccc tgcaagctgt gataggttca aaaataattg ccagaggtaa
     7861 tgttagagcc atgaggaaaa atgtaaccgc aaagtgttat ggcggtgatg tcactcgaaa
     7921 aaagaaattg ttagaaaaac aaaaagcagg taagaaaaga atga
//
LOCUS       DASCTH010000045         4774 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000065544, whole genome shotgun sequence.
ACCESSION   DASCTH010000045 DASCTH010000000
VERSION     DASCTH010000045.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000065544"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="QGH35_00985"
     CDS             complement(<1..292)
                     /locus_tag="QGH35_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HJL81140.1"
                     /translation="MQTTKTMMAFFSLLLFFLTQNINAFELTPGTGYLKASRKITCSL
                     IDDQPIVYWWYGRAYSRVPGEKDRLLFRVEGMNIRQCTTLEDEERGLGFRMVS"
     gene            complement(324..1412)
                     /locus_tag="QGH35_00990"
     CDS             complement(324..1412)
                     /locus_tag="QGH35_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012993.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HJL81141.1"
                     /translation="MRTLLTLMIIIFALGSLAVESKLVHQDGSYEMIPLEKENIVIKV
                     IQTGANSLQDFEDPKEGLKQNLDHMISMANKACGRGSKPDFLLFHEFPLTGYSSGSRK
                     EKLKYTIQVPGPETKALGKIAKECDTYLIFGSYATDSDWPQHILSLNTVIGRNGEVMK
                     AFWKSRNIKRIYKDREITTTTIEAVRDKYREKYGIDEEFPVLRTEFGNFAVTTVQGDP
                     FVFAAFAMKGVEIMFRTATLFSESDVLAMAWSNNFYTAMANITIPLGTPYSDAGGRSL
                     IASPDGNIIIQDPSTHEEGIISAEIPIAAFRKNRKIPNYALEMVQPIFLQYQQEIPIN
                     HLDIPANELPQTGEEMKELFDAISRYLN"
     gene            1537..3942
                     /locus_tag="QGH35_00995"
     CDS             1537..3942
                     /locus_tag="QGH35_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81142.1"
                     /translation="MTIRESLSFLVNSAIMVVITIALMPTVTFSQEGIEEIVVTARKK
                     EETLQSIPLAVSAVTAAQIEAQQMHSIFDIAMFTPGLSNSKAFGRNTERPVIRGLSNV
                     LAGVRFGVEAGAAYFVDGVYYPGDIGSFNVNDIERVEVIRGPQSALYARNTYSGAINF
                     ITKRPGEVTSSSVSLRSAEHGETDINFSTSQRISDNLALSLSARSYDYDGEWTNLVTG
                     KTVGDESTDSYSLVLDYQNENFGMTFRHQTSDSDDGTRALFLQPGNMNNCYPTSTGEM
                     NYFCGEIKTRPIALNDEAFPGTGAELNALITSWGMPEVPLQSYGSGLQWFFYPTNSTE
                     DGLYFSGVARDMSLTLLSMDYEMESGVNVSMSMGVKDEDLATGSDSDHSPVNLVRNAY
                     GGYAQPNGQCYPFCASGIEYMEDDTYELMIDSNQDQAFRWRIGYFNYDREQEQRGWSF
                     IAAYHNLLEQTSTIGNSAIYGMIEYDISDVLTWSFEARSAEETKTLKEYDTNGVIVAD
                     EDGKWDSDTIRTTLTYTKDPNTLFYFTFAEGAKPGGFNGSAGTDNGVPIYAMETNENI
                     EIGIKKVWSDGRLVTNLAVYQIDATNVQVTQPAPGTIGTLTSIVVGEGNLGEVNGVEL
                     EVNWLMNDNWNLAFNYSLADSTYKEGCDNYQWALTSGGGSYDVSNPAASTNPNGQGTC
                     SIKGKQFAFSPKTTYSVVLSYNKQLANGSELFGGIDSSYESKKYAQVHNGAYVGAYNL
                     FGARFGIRMESGLTLTAYGKNLGDADEPDVVTRWLDNDYSRAFFSNKRRGRSIGVELK
                     YDF"
     gene            4008..>4774
                     /locus_tag="QGH35_01000"
     CDS             4008..>4774
                     /locus_tag="QGH35_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HJL81143.1"
                     /translation="MKNRRTIFIFIATLAMNPKRLMQLALMVGGLLITSAGLELKAAM
                     PGMAGDIQRSFSNTQFGRVHHWSIGQGSTLLMLHQSGQTSTEFLAIAPLLANSFRVVG
                     IDLPNHGQSDTSDHELTVDEYADAVVDVLDNMGITQAHMLGQHGGAAVVINLGIRYAD
                     RVGKVILSGAGRDEDVDEEKLQKMLNTPMTRDLPIDVNGDFLQKTWSVYRKMSASNTP
                     PEVTFQPFLSSLTLRQRRYDLHYAVYRYSPNLDEFNK"
BASE COUNT         1393 a          921 c         1041 g         1419 t
ORIGIN      
        1 gactcaccat tctaaaacct agaccacgtt cctcatcctc aagagtggta cattggcgaa
       61 tgttcattcc ctcaactcta aataatagtc tgtctttttc acctggtaca cgactgtagg
      121 ctcttccata ccaccagtaa acaatgggtt gatcatctat tagagaacag gtaatttttc
      181 tggatgcttt gagatagcct gtgccaggag ttagttcaaa tgcattaatg ttttgcgtta
      241 gaaagaacag gagtaaagaa aagaaagcca tcattgtttt agttgtttgc atattagttt
      301 cctatataaa agatattaat gggttaattg agatatcgac tgattgcatc aaacagttcc
      361 ttcatttctt ccccagtttg aggaagctca tttgcaggta tgtccaagtg atttatcggt
      421 atctcttgtt ggtattgcaa aaatatgggc tgcaccatct caagtgcata atttggtatt
      481 ttcctatttt ttctgaacgc agcaataggt atctcggctg aaataatgcc ctcttcatgg
      541 gtgcttggat cttggataat aatgtttcca tccggtgatg cgataagaga tctgccacct
      601 gcatcactat agggcgtgcc aaggggaatt gtaatatttg ccattgcggt gtagaaattg
      661 tttgaccagg ccatcgccaa cacatctgat tcagagaaaa gggtagcggt tctgaacata
      721 atctcaactc ctttcatggc aaaggcagca aaaacaaatg gatctccctg aacagtagtg
      781 acagcaaaat tgccaaattc ggttctgaga acaggaaatt cttcatctat tccatatttt
      841 tctcggtact tatcacgtac tgcttcaatg gtagtggtgg tgatctctct atctttgtag
      901 attcttttga tatttctgga tttccagaag gctttcatca cctcaccatt ccttcctatt
      961 acggtgttca gactgaggat atgctgaggc caatcagagt ccgtagcata actgccaaaa
     1021 ataagatagg tatcgcattc tttggctatt tttcctaagg ctttagtttc aggtccaggc
     1081 acttgtatgg tgtatttcag tttttctttt cgacttccag aagagtaacc cgtcagggga
     1141 aactcatgga agagcagaaa gtcgggtttt gaccctctac cacaagcctt attggccatg
     1201 gaaatcatat ggtcaagatt ctgtttgaga ccttccttgg gatcttcgaa gtcttgtaga
     1261 gagttagcac ctgtttgtat gactttgatt actatgttct ccttctccag tggaatcatt
     1321 tcatatgaac catcctggtg caccaattta gattcaacgg ccaatgaacc taaagcaaag
     1381 ataataatca ttaaagttag taacgttctc atagttatat tagtatgttt ctcaagattg
     1441 tatattctac aaaattaact tgtctgtcaa attgacagtc tcctgtcttt ttatgtagta
     1501 tacatgctaa tagttaaata cttaggggga tatctcatga ctataagaga aagtcttagt
     1561 tttttagtca actcagcgat catggtagtt ataacaattg ccttgatgcc aactgtcacc
     1621 ttctctcaag aaggaattga agagattgtt gttactgcaa gaaaaaagga agaaactcta
     1681 caatcaattc ctttggcagt ttccgctgta actgcagccc agattgaagc acaacaaatg
     1741 catagcattt ttgatatcgc tatgtttaca ccgggtctat ccaattccaa agcttttggt
     1801 aggaatacag aaagaccagt tattcgagga ctatcaaacg tattagctgg tgttcgtttt
     1861 ggtgtagagg ctggagctgc atattttgta gacggtgtct attatcccgg tgatatcggt
     1921 tcatttaatg tcaatgatat tgaaagggtc gaagtcattc gaggccctca aagcgctctt
     1981 tatgcaagaa atacctactc aggtgccatc aacttcatca ccaagagacc gggtgaggtt
     2041 accagttcaa gtgtatcgtt acgatcagcg gaacacggtg aaacggatat taatttctca
     2101 acttcacaaa gaatctcaga taatttggca ctttctttat ctgcaagaag ttatgactat
     2161 gatggagaat ggaccaatct tgtgactggc aagacagtag gtgatgaatc cactgattcc
     2221 tactccttag tattggatta tcaaaatgag aattttggaa tgacgttcag acatcaaaca
     2281 tctgattctg atgatggaac cagagcactt tttttgcaac cgggcaatat gaacaattgt
     2341 tatccaacga gcacgggaga aatgaattat ttctgtggtg aaattaagac ccgacccatc
     2401 gctctcaatg atgaagcctt tccaggaaca ggagcagaac ttaatgcctt aatcacttct
     2461 tggggaatgc cagaggtacc actgcaaagt tatggtagcg ggcttcaatg gtttttttac
     2521 cccactaaca gtaccgagga tggtttatac ttttctgggg ttgcacgaga tatgagtctg
     2581 actcttctct ccatggatta tgaaatggaa agcggtgtca atgtttcgat gtccatggga
     2641 gtgaaagatg aagatttagc aaccggttcg gacagtgatc attcacctgt caatctggtt
     2701 agaaatgcat atggtggcta tgctcaacca aatggtcagt gctatccatt ttgtgcctcc
     2761 gggattgaat acatggaaga cgacacctac gaattaatga tcgattccaa tcaggatcaa
     2821 gcattcaggt ggcgaattgg ctactttaac tacgacagag agcaagaaca gcgtggttgg
     2881 tcttttatag ctgcatacca taacttactc gagcaaacct ccaccattgg aaactcagct
     2941 atttatggaa tgattgaata cgatatttca gatgttttga cttggagctt cgaggcacgg
     3001 tctgctgaag agacaaaaac actgaaagaa tatgatacta acggagtcat tgttgcagat
     3061 gaggacggca aatgggattc ggatacgatt cgaaccacgc tgacttacac aaaagaccca
     3121 aacactctgt tttacttcac ttttgcagaa ggagcaaaac ccggtggctt taatggttcg
     3181 gctggaaccg ataatggggt accgatctat gcgatggaga ccaatgagaa tattgaaatt
     3241 ggaatcaaga aagtttggtc tgatggaaga ctggtgacca atttggcagt gtatcaaatt
     3301 gatgcaacaa acgtccaagt gacccaacct gcaccgggta ctattggaac acttacatcg
     3361 atcgttgtag gggaaggtaa tttaggagaa gtcaatggag ttgagttaga ggtcaattgg
     3421 ctgatgaatg acaattggaa tctggcattt aattactcgc tagcagattc cacttacaag
     3481 gaaggatgtg ataactatca atgggcactt acatctggtg gaggtagtta tgacgtaagc
     3541 aatcctgccg cctcgactaa tccgaacgga caaggcactt gttccattaa aggtaaacag
     3601 tttgccttca gtccaaaaac tacttattcc gtagttttgt cgtataacaa gcaactcgcc
     3661 aatggttcag agttgtttgg aggcattgat agttcttatg aatctaagaa atacgcccaa
     3721 gttcacaatg gtgcctatgt tggtgcctat aatttatttg gtgctcgatt tggcattagg
     3781 atggaaagtg gtttaaccct aactgcatat ggtaaaaatc taggggatgc tgacgagccg
     3841 gatgtggtga caaggtggct cgataatgat tactcaaggg cctttttctc aaataaaaga
     3901 agagggcgct ctatcggtgt cgagttaaaa tatgattttt aaattctgaa aggattccct
     3961 caagaatggc cctatagata catcatctat agggtcgttt gtttataatg aaaaatagaa
     4021 gaacaatatt tatcttcatt gctactcttg cgatgaatcc caaacgattg atgcaattgg
     4081 ctttgatggt cggtggtctc ctaattacct cagctggtct tgagttaaaa gcagccatgc
     4141 ccggcatggc tggggacatc cagcgatcat tttccaacac acagtttgga cgggttcacc
     4201 actggagtat tggtcaaggc tcaacgttgc tgatgctaca tcagtccgga caaacatcaa
     4261 cagaattcct agcaatagca cctctattgg caaattcttt cagagtagtt ggcatcgact
     4321 taccaaacca tggtcaatcc gatacttctg atcacgaact tacagtagat gaatatgccg
     4381 atgcagtggt tgatgtacta gataatatgg gcatcactca agctcacatg ctgggtcaac
     4441 atggaggtgc tgcagtggtg atcaatcttg gtatacgcta cgcagataga gtaggaaaag
     4501 taattctgtc tggtgcaggt cgtgatgaag atgtggacga agaaaaatta caaaagatgt
     4561 taaacacacc tatgacgcgt gatttaccta tagatgtaaa tggggatttt cttcaaaaaa
     4621 cttggtctgt gtatcgtaag atgagtgcca gcaatactcc tccagaggta acgtttcaac
     4681 ctttccttag tagcttgact ctacgacaaa ggcgctatga tttacattac gcagtgtatc
     4741 gttattcacc aaatcttgat gaatttaata aaga
//
LOCUS       DASCTH010000046         2218 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000066762, whole genome shotgun sequence.
ACCESSION   DASCTH010000046 DASCTH010000000
VERSION     DASCTH010000046.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000066762"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="QGH35_01005"
     CDS             <1..915
                     /locus_tag="QGH35_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HJL81144.1"
                     /translation="DDNFKLKGMITAKDFQKASDFPLAAKDQNGALLVGAAVGVGEES
                     YTRIDQLHEAGADVIVIDTAHGHTNRVISMVKSTKKKYPDMQLIAGNIATSEAAIALA
                     DAGVDAVKVGIGPGSICTTRIIAGVGMPQVTAILNVAQALSSKNIPVIADGGIRYSGD
                     LAKAIASGADTIMMGSLFAGTDESPGEVELYQGRTYKSYRGMGSIGAMAQQYGSKDRY
                     FQDNVEATEKLVPEGIEGRVPCKGSLSNIVDQLIGGLRQSMGYTGCKNIKELQENTEL
                     IRITDAGKKESHVHDVTITKEPPNYTLD"
     gene            942..>2218
                     /gene="guaA"
                     /locus_tag="QGH35_01010"
     CDS             942..>2218
                     /gene="guaA"
                     /locus_tag="QGH35_01010"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HJL81145.1"
                     /translation="MTTSNSNIDVKSQKILILDFGSQYTQLIARRVREAGVYSEIYSY
                     DTDISFIREFAPSGLILSGGPQSVFTEEAPQADQAIFDLNRPILGICYGMQAMAEQLG
                     GRVQSSANREFGYAEIQMQNSNRLFAGINQENLDVWMSHGDRVEVMPDDFQSIASTDN
                     APIAAMCDDNRSLYGLQFHPEVTHTEKGQTIIENFVHKICGCTDHWNPGNIIESDIKR
                     IREEVGNGQVLLGLSGGVDSSVVAALLHKAIGDQLTCIFVDTGLLRLDEGDQVMEVFA
                     SHLKVNVIRVNAEDLFLQRLSGVADPEEKRKIIGNVFIEIFEEEASKLENVDWLAQGT
                     IYPDVIESAGAKTGKAEVIKSHHNVAGLPERMSLKLIEPLRDLFKDEVRAIGKELGLP
                     DQLINRHPFPGPGLGVRILGCVEKEYADLLRLA"
BASE COUNT          689 a          407 c          536 g          586 t
ORIGIN      
        1 gatgataatt tcaaactcaa aggcatgatc accgcaaagg attttcaaaa agcaagtgat
       61 tttcctttgg cggcaaaaga tcagaatgga gcccttttgg taggagctgc agttggcgtt
      121 ggagaagaat catacacacg catagatcaa ttacatgaag ctggagcaga cgttattgtg
      181 atcgatacag ctcatggaca cacaaaccgt gtcatcagca tggttaagtc aacaaagaaa
      241 aaatatcccg atatgcaatt aatagccggt aatatcgcca catctgaagc agcaattgct
      301 ttagcagatg cgggtgttga tgcagtcaag gtgggtattg gaccagggtc catatgcacc
      361 actcgtatta ttgctggagt tgggatgcca caggtgacgg ctattttaaa tgtagctcag
      421 gccttgtcat ccaagaacat acccgtcata gcagatggtg gcataagata ttcaggtgat
      481 ctggcaaagg ccatagcatc aggtgctgat acgatcatga tgggcagttt gtttgcaggc
      541 acagatgaat caccaggcga ggtagaactg tatcagggta gaacctacaa atcatatcga
      601 gggatgggat caattggtgc catggctcag caatatggtt ctaaagatcg ttattttcag
      661 gacaatgttg aggcaactga aaaactcgtt ccagaaggca ttgaaggacg cgtaccctgc
      721 aaaggaagtt tgtcaaacat agtcgatcaa ctcattggcg gactgagaca atcaatggga
      781 tataccggtt gcaagaacat caaagagtta caggagaata ctgagcttat tcgaatcaca
      841 gatgccggga agaaagaaag tcatgttcac gacgtgacta tcaccaaaga gccaccaaac
      901 tatacgcttg actgattaat cttcattgca gaggccaata tatgacgaca tcgaattcta
      961 acatcgatgt aaaatctcaa aaaatattga ttcttgattt tggatctcaa tacactcagc
     1021 ttattgctcg cagagttagg gaagcagggg tttatagtga aatttattct tatgataccg
     1081 acatctcatt catcagagaa tttgctcctt caggattgat actatcgggc ggtccacaat
     1141 cagtattcac agaggaggca ccgcaggcgg atcaagcaat ttttgatttg aaccgaccaa
     1201 tattgggcat ttgttatggg atgcaagcca tggctgaaca attgggtggc agagtgcaat
     1261 cgtctgccaa tcgggagttt ggttatgcag aaattcagat gcaaaactca aatcggttat
     1321 ttgctggaat caatcaagag aatcttgatg tttggatgag tcacggtgac agagtagagg
     1381 tcatgcctga tgattttcaa tcaatagcat ccactgacaa tgcacctata gctgccatgt
     1441 gtgacgataa cagatcattg tacggactcc aatttcatcc tgaggttacc catactgaga
     1501 aaggacaaac tattattgag aattttgtac ataaaatctg tggatgcact gatcattgga
     1561 atcctggcaa tatcatcgaa agcgatatca agcgtattag ggaagaggtt ggtaatggtc
     1621 aagtgcttct aggactttct ggcggagtag attcatcagt agttgcagcg ttactacata
     1681 aagccattgg tgatcaattg acctgtattt ttgttgatac cggtttgctt cgattggatg
     1741 aaggagatca agtgatggaa gtgtttgcca gtcatttaaa agtcaacgtg attcgtgtga
     1801 atgccgaaga cttattttta caaagattaa gtggtgtggc tgatccggag gaaaagagga
     1861 aaatcatagg gaatgtcttt atagaaattt tcgaggagga agctagcaaa ttagaaaatg
     1921 ttgattggct tgcccaaggt acaatttatc ccgatgtcat agaatcggct ggtgcaaaga
     1981 ctggcaaagc tgaagtgata aaatcccacc acaatgttgc tggtctgcca gaaagaatgt
     2041 ctctaaaact cattgaacca ctacgagatc tgtttaaaga cgaagtgaga gcaattggca
     2101 aggaattagg tttaccagat caactgatta atcgccatcc attcccagga cccggtttgg
     2161 gcgtgagaat actcggctgt gttgaaaaag aatacgctga tcttttaagg cttgcaga
//
LOCUS       DASCTH010000047         2030 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000067721, whole genome shotgun sequence.
ACCESSION   DASCTH010000047 DASCTH010000000
VERSION     DASCTH010000047.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000067721"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="QGH35_01015"
     CDS             complement(<1..712)
                     /locus_tag="QGH35_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020037402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HJL81146.1"
                     /translation="MKTKSIAQQLVTRLNTRSEDYVENTNMMKVKLAEIDKLLDLAEA
                     GGGEAHHERLAKKGKMPIRERIANVLDPDSPFLEISPLAAYGSSYTIGGGAVSGIGVI
                     AGVECVIFGNDPSVLAGALTPYAAKKWIRSLEVARENNIPYICFVESAGADLRIDPTK
                     VSEPNIGLGHFAETGRAFYDTIELSKMKVPTVCVVFGSSTAGGAYQPGMSDYNIFIKE
                     KSKVFLAGPPLVKMATGEE"
     gene            814..1629
                     /locus_tag="QGH35_01020"
     CDS             814..1629
                     /locus_tag="QGH35_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HJL81147.1"
                     /translation="MKKITRDIMEAVSDLNEILESLTDHQWKSPTAFKQWTPAEVVAH
                     LFYFDRMTLYSVNAPDTFNDEASYMFKTFSTLPNSLERAVAIKQRLGLDNNESLRRQW
                     YDSAMDMCSIFQVIDPAKRCTWFGPDMGARMFMTARFMEIWSHSQAIYDLIGKERVYK
                     ENIKNIATIGVKTFGWTYMNRKLEVPNQIPFISLSSPSGIQWQWNEPSTEESIKGLAS
                     DFCHVVTQTRNIDDTDLQLVGAVAKQWMAMAQCFAGDPEDPPAKGLRTKQKRG"
     gene            1634..>2030
                     /locus_tag="QGH35_01025"
     CDS             1634..>2030
                     /locus_tag="QGH35_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL81148.1"
                     /translation="MQWTEEHESIRETAAKFIDNEINPYVDQWEEAGMFPAKELFKKL
                     GDLGMLGISKPTEFGGLGLDYSYSMVFTEELGRIHCGGVGMGIGVQTDMATPALAKFG
                     SDELRNEFLVPAIAGDAVCSIAVSEPQAGS"
BASE COUNT          616 a          416 c          439 g          559 t
ORIGIN      
        1 attcctcgcc cgttgccatt ttaaccaatg gaggccctgc taaaaaaacc ttggattttt
       61 ctttaatgaa aatattgtaa tcggacatac cgggttgata agcaccacca gcggtggatg
      121 agccaaaaac aacacaaaca gtcggaactt tcattttaga caactcaatc gtatcgtaaa
      181 aagccctgcc tgtttcggca aaatgaccca atccgatatt gggttcactc acttttgtag
      241 ggtcaattct aaggtcagcg ccagctgatt cgacaaaaca aatgtaggga atattgttct
      301 ctctggcaac ctctaatgat ctaatccact tcttggcagc ataaggtgtt agtgcaccgg
      361 ctagaacaga tgggtcatta ccaaaaatga cacattcaac tccggcaata acaccaattc
      421 ctgatactgc acccccgcca atagtatagc tggagccgta agctgccaat ggactgattt
      481 caagaaaagg tgaatcggga tccaacacat tggcaattct ctccctaatg ggcattttac
      541 cctttttcgc caatctctca tggtgtgctt cgccaccgcc ggcttctgcg agatctaata
      601 gcttgtctat ctcggcaagt tttaccttca tcatgttagt gttttctaca tagtcctcag
      661 accttgtatt taaacgagtc actaactgtt gagctatgct ttttgttttc atcgacatat
      721 ccttatggta atattataat ataaatatta ccaatagaat aagataaatt ttagtatgtg
      781 aataagctcc tttgtaaaaa aactgacatt ctgatgaaaa aaataactag agacattatg
      841 gaggcggtat ctgatcttaa tgaaatctta gaatcattga ccgatcacca atggaaatca
      901 cccactgctt ttaaacaatg gacacctgca gaagtggtag ctcatttatt ctattttgac
      961 cggatgaccc tctactcggt taatgctcca gatacattta acgatgaagc gagttatatg
     1021 tttaaaacat tcagcaccct tcccaattca ctggagagag ccgtggcgat aaagcaaaga
     1081 ttaggactag ataataatga gagcttaagg cgtcagtggt acgattctgc gatggacatg
     1141 tgctcgatat ttcaagtcat tgatccagca aaaaggtgta cttggtttgg acctgatatg
     1201 ggtgccagaa tgttcatgac agcccgtttt atggaaatct ggtcacacag ccaggccatc
     1261 tatgatttga taggaaaaga gagagtctac aaggaaaata taaagaatat agcaaccatt
     1321 ggagtcaaaa cctttggttg gacttatatg aatcgaaagt tggaagtacc caatcaaatc
     1381 cccttcatct ccttatcgtc cccttctggt atccagtggc aatggaatga accaagtact
     1441 gaggaatcga ttaaaggact tgcaagtgat ttttgtcatg tagtgacaca aacaagaaat
     1501 attgatgata ccgatctgca acttgtggga gcagtcgcta aacagtggat ggctatggca
     1561 caatgttttg caggtgatcc tgaagatcca cctgcaaagg gattaagaac taaacaaaaa
     1621 agaggataag aaaatgcaat ggacagaaga acatgagagc attcgcgaaa cggctgctaa
     1681 atttattgat aacgaaatca atccctatgt ggatcagtgg gaagaagctg gtatgttccc
     1741 tgcaaaggaa ctctttaaaa agctaggtga ccttggcatg cttgggatta gcaagcccac
     1801 tgaatttggt ggcctcggtc tggattactc ttatagcatg gtttttaccg aagagttggg
     1861 ccgcatccat tgcggaggtg taggcatggg catcggtgta caaaccgata tggcaactcc
     1921 tgctttggca aagttcggta gtgatgaatt gcggaatgaa ttcttagtac cagccattgc
     1981 tggagatgct gtttgctcga ttgcagtcag tgaaccacaa gcgggctcgg
//
LOCUS       DASCTH010000048         4576 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000069879, whole genome shotgun sequence.
ACCESSION   DASCTH010000048 DASCTH010000000
VERSION     DASCTH010000048.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000069879"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="QGH35_01030"
     CDS             complement(<1..272)
                     /locus_tag="QGH35_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81149.1"
                     /translation="MSSTETDLKLSWSGISTSITHEWLEEFRVAVEMQKSQAFREYRV
                     ARIIELSQKQLDEFEENKDSDDFMQTLAWNIGELYCWQEDGEIIEPP"
     gene            399..1304
                     /locus_tag="QGH35_01035"
     CDS             399..1304
                     /locus_tag="QGH35_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019925582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4437 domain-containing protein"
                     /protein_id="HJL81150.1"
                     /translation="MRAHVELIQQSDLCWHPAELHGGTGRVMQRNLSYDEEDGSASTK
                     LFFETAWDRPGGYHEADIEWFVLEGRVKMGDTIFGPGMYWRAPAGLKIPAIAVDKDSE
                     ILVFREYGDWGFTVSETDKSDFIAEGGNTGSDQPGQLTVVDSSKMEWMDNIYEGDTQR
                     FLQLKILYHDPSPEGENEKGFITILGWAPPGWSDTRLIHHPVFEEAYTLTGYMDYNFG
                     RFVPGTYFFRPARVKHGHFVAGEEMGASWLFRLDGSLINWVTVNSQVIVKGDALNYDP
                     KTQAPLMAGIPVRSRSTGPWDLEGQ"
     gene            1357..2211
                     /locus_tag="QGH35_01040"
     CDS             1357..2211
                     /locus_tag="QGH35_01040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81151.1"
                     /translation="MDGIIDIVVNLFTPQEVELGQTGLDEDFKKQVRMPEKMRSGVTI
                     DQYLAKMDHAGIERSLLIAVRAGDIRVPESFEIPYSRVHEICQQHPDRFSGLAGIDPF
                     RGMVGINDLEHAVKEMGFVGAHLYPHWCELPPDHAKYYPYYAKCAELSIPIMMQVGHN
                     LIYSKHRRLPSVGRPICLDQVAIDFPELRLIGIHIGIPWTEEMISMCWKHENVYMAGD
                     AYAPKYWPDSFIHYANSYGKTKVMFGTDWPVIDPVRAVEEIENIKFKDESKKLIMRDN
                     ALRVFDLN"
     gene            2224..3759
                     /locus_tag="QGH35_01045"
     CDS             2224..3759
                     /locus_tag="QGH35_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012711.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HJL81152.1"
                     /translation="MANNTVFPSTFQHLSIASGIRAAMSRNPEKQCFKHGQHTRSYKD
                     LVSRMDRVSSGLINDLKLQPDDHAAIVAVNSIEYMEIILGASQANIAVATINPKLSVT
                     EIIAICDDAEAKVLFLDATMIENLHGAEFRTIKKIISIADDLEQWLQKCKPMMEYPVV
                     NEWDVFTIPYTSGTTGKPKGVLVPHRSRVLTLFGMAVEYGCYSPEDRFLAIAPMCHGA
                     GMIFSLAPIFFGGYAEIMDRFDPEEIVTTFKKENITGFFGVPTHFHGITGLETSILTK
                     NQSSSLNTIISNAAALPQTTKEKIVEIFGDNILHETYGSTEGGIIANLRPPDQLRKEQ
                     CVGQAFPCTATKILNVSGEECAPDEVGELFSTSPYLFNGYWNKPEETEEAIQNGWLTV
                     GDLAKKDSEGYLYIVDRKKDMVISGGINIYPREIEEVLFSHPDIIDAAVVGVADEKWG
                     ESIKAFLVTRNNIDLNLEAVRAFCEDKISTIKMPKIVSIIDEIPRNVGGKVLKTSLRK
                     LNQ"
     gene            3756..4406
                     /locus_tag="QGH35_01050"
     CDS             3756..4406
                     /locus_tag="QGH35_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013488.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HJL81153.1"
                     /translation="MTNKTIMVDYYFDYRCPWTYLSFKRLIQTTTRTASKINWKPVAL
                     KMINETLNPDNNENQSCNLSKMSYSRKDLQDWSTYCGLSITPRNDVLEDISIKALCGA
                     VYAIKNKKVKDYSSLVFQAHFSNQQDISDDDVLLDIAKRIELNLTDFKTSISSKNNLK
                     HLQDNTNELIQRGGFGSPTMFVDNDMYYGNDRMPLVEFSIGRASGKILVLPGQHDT"
BASE COUNT         1470 a          839 c          946 g         1321 t
ORIGIN      
        1 ggtggttcaa taatttcacc atcttcttgc cagcaatata actcgccaat attccatgca
       61 agtgtttgca taaaatcatc tgaatcttta ttttcttcaa actcatcgag ctgtttttga
      121 gacagttcta tgattctagc cactctatac tctctaaatg cttggctttt ttgcatctca
      181 actgcaaccc taaactcctc taaccattca tgcgttatag aggtgcttat tccagaccac
      241 gataatttta aatctgtttc agttgatgac atatttatct ctctcaataa aaataaataa
      301 ccactctgat tatcatttaa taaaaaaaat ttaacaactc tttatcaaac aaaaactttc
      361 atatattctt aagtcaatct aataaaaaaa tcaaggcaat gagagctcac gtcgaactaa
      421 tacaacaaag tgatttatgc tggcacccag ctgaacttca tggtggaacg ggaagagtaa
      481 tgcaaaggaa tctttcttat gatgaagaag atgggtctgc ttctacaaag ctttttttcg
      541 aaactgcatg ggatcgcccc ggaggatatc acgaggcaga catagaatgg ttcgtcttag
      601 aaggacgggt taaaatggga gatactattt ttggtccagg aatgtattgg cgagctcctg
      661 ccggtcttaa aataccagca attgctgtag acaaagacag tgagatattg gtatttcgag
      721 aatatggaga ttggggattt accgtctctg aaaccgataa aagcgatttt atagctgaag
      781 gggggaatac cggatcggat caaccgggac aactgactgt tgtcgactca tcaaaaatgg
      841 aatggatgga taatatctac gaaggagaca cgcagcgttt cttgcagctt aaaattctct
      901 atcatgatcc atccccagaa ggtgagaatg aaaaaggatt tattaccata ttgggatggg
      961 caccaccagg ttggtcggac acacggttaa tacaccaccc tgtttttgaa gaagcctata
     1021 cattaactgg ctatatggat tacaatttcg gtcgatttgt gccgggaaca tattttttta
     1081 ggcctgcacg agttaaacat ggtcattttg ttgctggaga agaaatgggt gccagctggt
     1141 tgtttagact agacggttct ttaattaatt gggttactgt aaattcacaa gtcattgtca
     1201 aaggcgatgc actcaactat gatccaaaaa cgcaagctcc attaatggct ggcattccag
     1261 ttagatctcg ctccactggt ccttgggatc ttgaaggtca atagttgata aaaacctata
     1321 taaattaaac agattcaata gaaatggatg attaatatgg acggtattat tgatattgtc
     1381 gtgaatttat ttacacctca agaagttgag ctaggccaaa ccggcttaga tgaggacttt
     1441 aaaaagcaag ttcggatgcc agaaaaaatg aggagcggcg taaccataga tcaataccta
     1501 gcaaaaatgg atcatgcggg gattgaaaga tctttactaa tcgcagtaag agctggtgat
     1561 ataagagttc cagaatcttt tgaaattcct tattcaagag tccatgagat ttgccaacaa
     1621 caccccgatc gattctcagg cttagctggt attgaccctt tcaggggtat ggttggaatc
     1681 aatgacttag aacatgcagt caaggaaatg gggtttgtag gagctcattt ataccctcat
     1741 tggtgtgaat tacctccgga tcacgccaaa tattaccctt attatgcaaa atgtgctgaa
     1801 ctcagcattc ccatcatgat gcaggttgga cacaacctta tttattcaaa acataggaga
     1861 ttaccaagtg tgggcaggcc catttgtctt gatcaggttg ccattgattt tccagaatta
     1921 agactcattg gtatccatat tggtatacct tggacagaag aaatgatttc tatgtgttgg
     1981 aagcatgaaa atgtttacat ggcgggtgat gcctacgccc caaagtattg gcctgattca
     2041 ttcattcatt atgcaaattc atacggcaag acaaaagtta tgtttggcac tgattggccg
     2101 gttattgacc ctgttagagc agttgaggaa attgaaaaca taaaattcaa ggatgaatca
     2161 aaaaaattaa ttatgagaga caatgcttta agagtatttg atctaaatta gaaattcttt
     2221 caaatggcaa acaacacagt tttcccttca acgtttcagc acttatccat tgctagtggc
     2281 attcgggcag cgatgtccag aaatccggaa aagcaatgtt ttaaacatgg tcaacataca
     2341 agatcttata aagatttggt ttccagaatg gatcgggtta gcagtggttt aattaatgat
     2401 ttgaaacttc aacctgatga ccatgcagct atagttgcag tgaactctat tgagtatatg
     2461 gaaatcattt tgggagcttc acaagcgaat attgctgtgg cgactattaa cccaaaactc
     2521 tcagtgactg aaattatagc aatctgtgat gatgcggaag ctaaagtatt gttccttgat
     2581 gcaactatga ttgagaacct ccatggtgct gaatttagaa ccataaaaaa aattattagt
     2641 atcgctgatg atcttgagca atggcttcaa aaatgcaaac caatgatgga atatcctgtg
     2701 gtcaatgagt gggatgtttt taccatccca tatacctctg gtacaactgg caagcccaaa
     2761 ggtgtattgg ttccacatcg ttcaagagtt cttacattat ttggcatggc agtggaatac
     2821 ggatgttatt ctccagaaga tcgcttccta gctatcgctc caatgtgtca cggtgcgggt
     2881 atgatttttt cactggcacc aattttcttt ggtggctatg ccgaaattat ggatcgattt
     2941 gatcctgagg aaattgtcac aacttttaag aaggaaaaca taaccggatt ttttggggta
     3001 cccacacact ttcatgggat aacaggatta gaaacatcaa tattgacaaa gaatcagtca
     3061 agctctctta atactattat ttccaatgca gccgcactgc cgcaaacaac caaagaaaaa
     3121 atcgttgaaa tttttggcga caatatttta catgaaactt acggttctac tgaggggggc
     3181 attattgcaa acctgagacc accagatcaa ttaagaaaag aacaatgtgt tggtcaagct
     3241 tttccttgca cggccactaa aattctcaat gtttctggtg aagagtgtgc gccagatgaa
     3301 gtgggtgaat tattctcaac tagcccttat ctattcaatg gctactggaa caaacccgaa
     3361 gaaactgagg aagcgattca aaatggctgg ctgactgttg gcgatttagc taagaaagat
     3421 agcgagggtt acttgtacat tgttgatcgt aaaaaagaca tggtgatttc cggggggatt
     3481 aatatatacc ctagagaaat agaagaagtt ttattcagcc accccgatat tatcgatgct
     3541 gcggttgtgg gagttgctga tgagaaatgg ggtgaatcca tcaaggcttt tcttgtgacc
     3601 cgaaacaata ttgatttaaa tctagaggca gttagagcct tctgtgaaga taaaatttca
     3661 accatcaaaa tgccgaaaat agtaagcatt atcgatgaaa taccgaggaa tgtgggcgga
     3721 aaagtgctaa aaacatcttt gagaaaactt aatcaatgac taacaagaca ataatggtgg
     3781 attactattt tgactatcgt tgtccttgga cctatttaag ttttaaaaga ctgattcaaa
     3841 ctaccacaag aactgcttcc aaaatcaact ggaaaccggt ggcactaaag atgatcaatg
     3901 agacgctcaa tccagataac aatgagaatc agtcttgcaa tctatcaaag atgagctact
     3961 ccagaaagga tctacaagat tggtcgacat attgtggttt atcgataaca cctagaaacg
     4021 atgtgctaga agatatctcc ataaaagcat tatgcggtgc cgtttatgct ataaagaata
     4081 aaaaagtcaa agattacagc tcattggttt tccaggctca ctttagtaac cagcaagaca
     4141 tatctgatga tgacgtgctg ttagatattg ccaaaagaat tgaattaaat ttaacagatt
     4201 ttaaaacctc aatcagttca aaaaataatt taaagcactt acaggacaat acaaatgaat
     4261 tgattcaacg aggtggcttt ggttcgccta ctatgttcgt tgacaatgat atgtactatg
     4321 gcaacgacag aatgcccctt gtagaatttt ccattggcag agcgagcggt aaaattttag
     4381 tcctgccagg tcagcatgat acataactga atgaatatga tagattatta gggatgtata
     4441 aatctgcgaa aacaacgcga agaaagtttt tactcggcag tgcatccgca attggcctct
     4501 ctctgaacta ctcaaaacct gctatggcca accttagtga caccaaactc tatcgatctt
     4561 gggaagattt ggcgag
//
LOCUS       DASCTH010000049         2622 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000070903, whole genome shotgun sequence.
ACCESSION   DASCTH010000049 DASCTH010000000
VERSION     DASCTH010000049.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000070903"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="QGH35_01055"
     CDS             <1..306
                     /locus_tag="QGH35_01055"
                     /inference="COORDINATES: protein motif:HMM:NF039614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HJL81154.1"
                     /translation="VISTNTIKKEYQSTVKNKLSQTHSIIEITRAQILNFCGNCMEIK
                     GDNNDLSLIMSTRAYNAYSGAQKQLLLEHYKKIIHSNLTTIENYGGGSARCMMMELF"
     gene            314..1831
                     /locus_tag="QGH35_01060"
     CDS             314..1831
                     /locus_tag="QGH35_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81155.1"
                     /translation="MIKEHKSGIIIIFLILGSLLISELAAKENSSLLIINAKVIDGTG
                     SAARLESVRINDSRIQGLGQLRPLPGETILDATGLVLSPGFIDTHSHHDISIFQMPDA
                     LSAISQGITTIVVGLDGFSQVPLSGFFAKVKDNPLAVNIASYSGHNSLRNIVMGQDYQ
                     RFSTPKEIENMKNLLRLDMKAGALGLSTGLEYDPGIYSEETDEVVALLEVFPEDQGRY
                     ASHIRSEDVHLESALDEFIEISRKSKLPSQISHIKLARRDLWGQTSWMIDKLNAARQE
                     GLDITADIYPYTYWESTMRTLFAKRDFDNRDSAEFALTELVHPNQLTLSHYEPDPNLV
                     GKTIAEIAVMRKEDPVTVLMHLIQDDKTKTDSAIGVAMTQPDIDKLMQWEHSNICSDG
                     ALNNGHPRGRGAFARILANYVRSRGILGWENAIHKMTQLSALHMGFKNRGVIKSGAIA
                     DLVLFDPEKIQDNATMDDINRLSSGIINVWVGGELVYSDKKSTGLRPGQVIRRAD"
     gene            complement(1854..>2622)
                     /locus_tag="QGH35_01065"
     CDS             complement(1854..>2622)
                     /locus_tag="QGH35_01065"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HJL81156.1"
                     /translation="FGCNAVVFMPKGTSKARLEAIQAHGAQAEITELNYDETVLFSAQ
                     QAKENDWVLLQDSSWEGYQTVPKHIMQGYFSLVSEFADQTTEWPTHVIAQAGVGSFAA
                     SVFSYFLASSKALPKLILVEPTGAACYFNCIRIGDGEPHLTKELNTMMAGLACGQPSI
                     LAWDIIKSNCDAFVICEDEIAIKGMQLLANPISNDAVIVSGESGAVPIGFLQEICTNQ
                     RHRLIKEKLSLDNNSRVLIFSTEGDTDPKNYQEYSKL"
BASE COUNT          825 a          520 c          538 g          739 t
ORIGIN      
        1 gtgatttcta ccaacactat caaaaaagaa tatcaatcaa cagtcaaaaa taaattgagt
       61 cagacgcatt caattattga aattactaga gcccagatac taaacttttg tgggaattgc
      121 atggagatta aaggtgacaa taatgacttg agcctgataa tgtcaactcg agcatataat
      181 gcatattctg gagcacagaa gcaattactc ctcgaacact ataaaaaaat cattcactca
      241 aatctaacaa ctattgagaa ctatggtgga ggttctgcaa gatgcatgat gatggaactt
      301 ttctagattg actatgataa aagaacacaa atctggaatc attattatat ttctgatttt
      361 aggatcgtta ctgatcagtg agttggcagc caaagagaac tcgtccttac tgatcatcaa
      421 tgcaaaagtc atcgatggaa ctggaagtgc tgcccgatta gaatctgtga ggatcaatga
      481 cagccggatc caaggacttg gtcaattaag acctctacct ggtgaaacaa tactagatgc
      541 tacaggacta gttctatctc caggattcat tgatacgcat agccatcacg atatttcaat
      601 tttccagatg cctgatgccc tttcagcaat cagtcagggg ataactacca tagtcgtggg
      661 tctggatggt ttttcacaag tacctctctc aggcttcttt gccaaagtga aagataatcc
      721 gcttgcggtc aatattgcct catactctgg gcacaattca cttcgaaata tagttatggg
      781 acaggactat caaagatttt ccacaccaaa agaaattgaa aacatgaaaa accttctccg
      841 attagacatg aaagctggtg cgttagggtt atcaactggg ctggaatatg atccgggtat
      901 ttactcagaa gaaaccgatg aggttgttgc cttactggaa gttttcccgg aggatcaagg
      961 acgatacgcc agccatatcc gcagtgaaga tgttcatttg gaatcagcac ttgatgaatt
     1021 tatagagatc agtcggaaat caaaacttcc atcgcaaata tcacatatca aacttgcgag
     1081 aagagattta tggggtcaaa cgtcttggat gatcgataaa ctgaatgctg ctcgccaaga
     1141 ggggctggat atcacagcag acatctaccc ttatacctac tgggaatcaa caatgagaac
     1201 actttttgcc aagcgagatt tcgataatag agatagtgct gaatttgctc taactgaatt
     1261 agttcatcca aatcagttga cactttctca ttacgaacca gacccaaatt tagtgggtaa
     1321 aactatagct gaaattgctg tgatgagaaa agaagatcca gttacggtgc tgatgcattt
     1381 gattcaagat gataaaacaa aaacagactc agcaattggt gtggctatga cacaaccgga
     1441 tatagataaa ttgatgcagt gggagcatag caacatatgt tcagatgggg cattgaataa
     1501 tggtcatccc agaggtcggg gtgcttttgc taggattcta gcaaattatg tacgctcaag
     1561 aggaattcta ggatgggaga atgccattca caagatgact caactttctg ccctgcatat
     1621 gggatttaaa aatcgaggtg taatcaagtc aggtgctatt gcagatttag tcttatttga
     1681 tccggaaaag atccaagaca atgccacaat ggatgatatc aacagattat catcgggtat
     1741 cattaatgtt tgggtaggag gtgaattggt ttactctgat aagaaatcaa caggcttgag
     1801 accgggacag gttatcagga gagctgactg aaagaattac aacaccagat ttcttataat
     1861 ttagaatact cttgataatt ttttggatcc gtgtcacctt cggtggaaaa tatcaatacc
     1921 cttgaattat tatccagtga aagcttttcc tttattaggc gatgtctttg atttgtgcat
     1981 atctcttgga gaaatccaat tggaactgcc ccactctcgc ctgagacgat aactgcatca
     2041 ttacttattg gatttgctaa caattgcatg ccttttattg caatctcatc ctcacaaatt
     2101 acaaatgcat cacaattgga tttgatgatg tcccatgcca gaatacttgg ctgaccacat
     2161 gcaagtcctg ccatcattgt attcagttct tttgttaaat gcggttcgcc atccccgatt
     2221 cgtatgcaat taaaataaca agctgctcct gttggctcca ctaaaattaa tttaggcaga
     2281 gctttagagc ttgccaagaa ataggaaaat accgatgcag caaaggaacc aacccctgct
     2341 tgggcaatca cgtgtgtcgg ccattcagta gtctggtctg caaattctga caccaagctg
     2401 aaataacctt gcataatatg tttgggtaca gtctggtatc cttcccaaga ggaatcttga
     2461 agcaataccc aatcattctc tttcgcttgt tgggcagaaa ataggactgt ctcatcataa
     2521 ttaagttcag tgatttcagc ctgagcccca tgtgcttgga ttgcctccaa acgagctttc
     2581 gacgtacctt tgggcataaa aaccacagca ttgcatccaa at
//
LOCUS       DASCTH010000050         2048 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000070931, whole genome shotgun sequence.
ACCESSION   DASCTH010000050 DASCTH010000000
VERSION     DASCTH010000050.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000070931"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            379..>2048
                     /locus_tag="QGH35_01070"
     CDS             379..>2048
                     /locus_tag="QGH35_01070"
                     /inference="COORDINATES: protein motif:HMM:NF019335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HJL81157.1"
                     /translation="MKLISRYFVGAVLTALIVSPLAMAQSIEEITVTARKKTENLQDV
                     PISVTAISSDVVERLGIKDIRDVVKMDSSLIFDKGFSPDDTRIVVRGIDNGRGRPVVA
                     NIFDGADISAEALNSAGASLLVSPRLIDIERVEIVKGPQVALYGRTAFAGAIQYVTKG
                     ASETPEATASVEIAQDGLHEMRFSASGPLTDKLGIRFNALSWDFDGFHTNTETGNKVG
                     GGEGSSASIMFDWNPSEKLHIRARLDHVSDENDPPAQAYVPFNSLAKVADAASWCNGG
                     WVHDASCKGYAEALETAMAWGAHDANYTGYGWQAVFGPWFTWQQSVPIFAGSWKINCA
                     GPPYIPSVAAPDPVYNTCSPFNAPGFASTGNQINSETGTLYGGRFDDMEHSVYVGAMP
                     DGDDLIITQDRNYAHPGNNKGSDMFGHTKEGERLQINLTYDLAVGTFSSITHIADMDV
                     TQQYDIDKRALSWFAQEWDVSGGTKLFSQEFRLQTENDGPFNMAMGALYWHEDTIQTS
                     TGLSIRGFGEQCQLIAIRDARGNLKSVSDLGASSWAGPCGHTDVNIRG"
BASE COUNT          552 a          424 c          506 g          566 t
ORIGIN      
        1 gatgtctttt tgaacacttt ttatgctctt gcagggctcg gtgttgtgtt gattgtggct
       61 tctcggttta ttaagggaga aatcttagaa gactagccaa aaatcttttt agtctcctga
      121 aacaaaactt aaccattaag ttgatctaga tcaattcata aatgacttaa accaactagg
      181 gttataagga caattcttaa tgataataaa gcacatctac ttgcgctctt aaaaaaagag
      241 cagcaaaaac agctctgttg tataataaca actgtctttt aattgtattg agcgcctgtt
      301 ttgtttggtt tttaacactg cctcagttac attaagataa gtatatattt agattactaa
      361 tttagggggt tttatttaat gaaactaatt agcagatatt ttgtcggcgc cgtgctgaca
      421 gcactcatcg tctcaccgct cgcaatggct caaagcattg aagagattac agtgacggca
      481 agaaagaaaa cagaaaatct tcaagatgtg cccatcagtg ttaccgctat aagcagtgat
      541 gttgtggagc gtcttggcat caaagacatt agagatgttg tcaaaatgga ctcatccttg
      601 atttttgaca aaggattttc acctgatgac acccggattg tggtccgagg catagacaac
      661 ggccggggtc ggccagtggt tgccaacatc ttcgatgggg ctgatatttc cgctgaagca
      721 ttgaattcag ccggggcttc gttgttggtc tcacccaggc tcatcgatat tgagcgcgtg
      781 gaaattgtta aaggtcccca ggttgctttg tatggccgaa cggcgtttgc cggagctatt
      841 cagtatgtaa ccaaaggcgc atcagagact cctgaagcaa ctgcgagtgt tgaaattgca
      901 caagacggtc tgcatgagat gcgttttagt gcttccggtc ccctcacgga taaattaggt
      961 attcgcttca atgcgctgtc atgggatttt gatggctttc acacaaacac tgaaactggt
     1021 aataaggttg gtggcggtga aggctcgagc gcctcgatta tgtttgactg gaacccgagt
     1081 gaaaaattac atattagagc cagactggat cacgtcagtg atgaaaacga tccacctgca
     1141 caagcatatg ttccattcaa ctcattagcc aaagtggctg atgctgccag ttggtgtaat
     1201 ggcggctggg ttcatgatgc ttcctgtaag ggttatgctg aagccttgga aacagcgatg
     1261 gcttggggag cgcatgacgc caactatacc ggatacggat ggcaggcagt gtttggccct
     1321 tggttcactt ggcaacaatc tgtgccgata tttgcggggt catggaaaat aaactgcgca
     1381 ggcccgcctt atatcccaag cgttgcggct ccagacccag tttataatac ctgtagccct
     1441 ttcaatgccc cgggttttgc cagcacaggt aaccaaataa acagcgagac aggcactctc
     1501 tacggggggc gctttgacga tatggagcat tctgtttatg tcggcgctat gcctgatggt
     1561 gatgatttga ttattaccca ggatcgtaac tatgcacacc ctggtaataa taaaggaagt
     1621 gatatgtttg gccacaccaa agaaggtgag cgcctgcaaa ttaatctcac ctatgatcta
     1681 gcagtgggca ccttttcttc catcacccat atagcagaca tggatgttac gcaacaatac
     1741 gatatcgata aacgagcttt gagttggttc gctcaagaat gggacgtttc agggggaact
     1801 aagttattca gccaggaatt taggctgcaa acagaaaacg atggaccatt taatatggcc
     1861 atgggtgcct tgtattggca tgaggacacc attcaaacct ctacgggatt gagtattaga
     1921 gggtttgggg aacagtgtca gcttatcgca atcagggatg ctagaggtaa ccttaagagt
     1981 gtttccgacc ttggcgcgtc ctcatgggca ggcccttgtg gtcacacgga cgtcaacatt
     2041 agaggctt
//
LOCUS       DASCTH010000051         1792 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000073164, whole genome shotgun sequence.
ACCESSION   DASCTH010000051 DASCTH010000000
VERSION     DASCTH010000051.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000073164"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="QGH35_01075"
     CDS             complement(<1..313)
                     /locus_tag="QGH35_01075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJL81158.1"
                     /translation="MEPYKRIREIYGKAAIDYDKFWDFYSAPSYSKTLARINLEEGSK
                     VLDLGCGTGELLKILEDCFPSSDLTGIDLTEEMLAIAKQKLSSRVELLLGSVTNLPFD
                     SE"
     gene            complement(330..1292)
                     /gene="thiL"
                     /locus_tag="QGH35_01080"
     CDS             complement(330..1292)
                     /gene="thiL"
                     /locus_tag="QGH35_01080"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HJL81159.1"
                     /translation="MLDVTEEKIIEALKEATKVPNQGVILGIGDDAAVAEIKPGHQLV
                     TATDVIALGTHYPEGIDPKSIGHRCLAVNLSDIAAMGATPLWANLIVSLPTVKLDWIN
                     KFIEGFSTIANRFNVALVGGDTICGPEFFAVSLQGEVYEDQYISRSGAQVDDLIYVSG
                     LLGDASYGLKIIKDKIATKGNQLLIDKFLYPNPRVEEAKTISSFASSMIDLSDGVYSD
                     LMKIMSESNVGVEVDVSKLPISVLLSDQLNREAAIHQALLGGDDYELCFTLPAAREME
                     FLKMHQSISDPSFTCIGKIRQGSDLKLFDGKEKFVISEKPYNHF"
     gene            complement(1306..1755)
                     /gene="nusB"
                     /locus_tag="QGH35_01085"
     CDS             complement(1306..1755)
                     /gene="nusB"
                     /locus_tag="QGH35_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HJL81160.1"
                     /translation="MMLKSNKKNKDVRLTKKGRSGSRQLLLQVLYQHQINGDQHHALL
                     TQSKKTKEFTRIDQEYFELLLKEILENTDTLDKTAFKFTDRSIKRLDPIERAILWIGL
                     CEFSFHKDTPSIVVINEAIELAKIFGAQDSFQFINGVLDSFLQEQIT"
BASE COUNT          541 a          366 c          309 g          576 t
ORIGIN      
        1 attctgaatc aaagggaagg tttgtaacgc tacctaaaag aagctcaact ctgctggaaa
       61 gcttctgttt tgcaatcgct agcatttctt cagtcaaatc aattccggtg agatcagatg
      121 atggaaaaca gtcctccaga atttttagca attctcctgt cccgcagccc aagtcaagaa
      181 cctttgaacc ctcttctaga ttaattctag caagagtctt tgaatacgag ggagctgaat
      241 aaaagtccca aaacttgtca tagtctatcg ctgccttacc ataaatttct ctgattcgct
      301 tatatggttc catgatgttt agcggtcact taaaaatggt tgtagggttt ctcagagatt
      361 acaaattttt ccttcccatc aaataacttt aaatctgatc cctgtcttat tttgccaatg
      421 caggtgaacg atggatcaga gatagactga tgcattttaa gaaattccat ttctctggct
      481 gcagggagag tgaaacataa ttcataatca tcgccaccta gaagtgcttg atgaatagca
      541 gcctctctat tcaattggtc agatagtaaa actgagatcg gtaattttga aacatcaact
      601 tcaacaccaa cattactctc agacataatt ttcattaaat cgctatacac accatccgat
      661 aaatcaatca ttgatgacgc aaacgaactt atggttttgg cttcctctac tctgggattg
      721 ggatataaaa atttatcgat caacaactga ttgcctttag tggcaatttt atctttaatg
      781 attttgagac cataggaagc atctcccaat agtcctgaaa catatattaa atcatcgact
      841 tgtgcaccag atcttgaaat atattgatcc tcatacactt caccctgtaa tgaaacagca
      901 aaaaactcgg gaccacaaat ggtgtcccca ccaacaagag caacattaaa tcggttagca
      961 atagttgaaa acccctcaat aaatttatta atccaatcta gttttacggt tggcaagctt
     1021 actattagat tggcccataa tggggttgct cccatggcag caatatcgct taaatttaca
     1081 gctaaacagc gatgcccaat cgatttagga tctatccctt cagggtaatg agtgcctaac
     1141 gcgatgacgt ctgtagcggt aaccaactgg tgcccgggtt taatttctgc tacagctgcg
     1201 tcatctccaa tccctaaaat tacaccctga ttaggcactt tggtggcctc ttttaaggct
     1261 tcgatgattt tctcttccgt cacgtcaagc atggacagtg tctgattaag ttatctgttc
     1321 ttgtaaaaat gaatccagta caccattgat aaactgaaaa ctatcttggg caccaaaaat
     1381 ttttgctaat tcaattgcct cattgatgac aacgatagaa ggggtgtctt tatgaaatga
     1441 gaattcacat agtccaatcc aaagaattgc acgttcaata ggatctaatc ttttaattga
     1501 acgatctgtg aatttaaatg cagttttgtc taatgtatct gtattttcaa gaatctcttt
     1561 caagagcagc tcaaagtatt cttggtcaat tctagtaaat tcttttgttt tttttgactg
     1621 tgtcaataat gcatggtgct ggtcgccatt gatctgatgc tgataaagaa cttgcagtaa
     1681 taattgtcta gaaccagaac gacctttttt agtcaatctt acgtctttat tttttttgtt
     1741 actttttaac atcatgcatt catcaatata ataagattac tctgcatcgt gt
//
LOCUS       DASCTH010000052         1855 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000073735, whole genome shotgun sequence.
ACCESSION   DASCTH010000052 DASCTH010000000
VERSION     DASCTH010000052.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000073735"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="QGH35_01090"
     CDS             complement(<1..668)
                     /locus_tag="QGH35_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013324.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HJL81161.1"
                     /translation="MRNILVVLLVAILLVISNSIFTVDEREMALKFRFGEVIRADYEP
                     GIHFKVPFVNNVGKYSKQILTINNPQELFLTLEKKNLFVDFFIKWKINDVQQYYISTG
                     GDELVASQRLLETVKDGIRSEFAKRTVIEVVSEERRKVMSEMLNAAALNAINLGIELV
                     DVRVKRVELSDEVSESVYNRMRQERARFASELRAEGAEAAAIIKAEADREVTVIIANA
                     YRDS"
     gene            complement(668..1783)
                     /gene="hflK"
                     /locus_tag="QGH35_01095"
     CDS             complement(668..1783)
                     /gene="hflK"
                     /locus_tag="QGH35_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HJL81162.1"
                     /translation="MAWNDQGNGKSPWDPNKNQPPDLDQIVEEWQKRFNKLFGMKSTG
                     SGKKPRGLPKSIIVILILFVWVISGLYQIDQSERGVVLRFGAYSETTMPGLRWHIPWP
                     IEGVEVVNVNVVNRFKQQTTMLTSDENIIVVDLVVQYRQADPTDYLFNVRDADVTLSE
                     VSESAIREVAGKSDLDFILTEGRTEIALLTQQLIQQTIDEYGTGIIITKVNLQDANFP
                     IQVEDAVQDAIKAREDKERVAFEAEAYANDIVPKSRGEAAKMIQDAEAYRARVIADAE
                     GEASRFSQLLAEYQKAPEVTRERLYIEAVESVYGNSNKVLMDSDGSGNLIYIPIDKLI
                     EQSKKKQSTNSEQGFSPQNDPSQTNQSSDLPRIRRVR"
BASE COUNT          490 a          424 c          292 g          649 t
ORIGIN      
        1 tcagaatccc tgtaagcatt ggcaatgata acggtcactt ctctatctgc ttctgcttta
       61 attatcgcag cagcttctgc accctcggct ctcaattctg atgcaaacct tgctcgctct
      121 tgtctcatgc ggttgtatac tgattcgcta acttcatctg agagttcaac tcttttaact
      181 ctcacatcga caagttctat cccaagattt attgcattta aagctgcagc attaagcatt
      241 tcagacataa ctttccttct ctcttctgaa accacttcta tgacagttcg ttttgcaaat
      301 tcagatctga ttccatcttt gactgtttcc aatagacgtt gtgatgccac cagctcgtcg
      361 ccaccggttg aaatgtagta ttgctggacg tcgttgattt tccatttaat aaaaaagtct
      421 acaaataaat ttttcttctc taaggtcaaa aacaactctt gaggattgtt gatggttaga
      481 atctgtttgg aatactttcc aacgttattt acgaagggta ctttaaagtg tatgccaggt
      541 tcataatcag ctctaataac ttcaccaaat ctaaatttaa gtgccatttc cctctcatcc
      601 acagtgaaga tactgttgct tatcactaga agaatagcga ccaaaagaac gactaggatg
      661 tttctcatta tcttaccctc ctaatccttg gcaaatcgga cgattggttt gtttgtgatg
      721 gatcattctg tggtgagaat ccctgttcag aatttgttga ttgtttcttt ttgctttgct
      781 caattaattt atctatcggt atataaatta aatttccact cccatccgaa tccatcaata
      841 ccttatttga attcccgtag acactctcaa cggcttctat atagagtctt tctctagtga
      901 cttcaggagc tttttgatat tcagctaata gctgagaaaa acggcttgct tcaccctctg
      961 catctgcaat aactctggct ctataagctt ccgcatcctg gatcatttta gctgcctcac
     1021 ctctggattt tggaacaata tcatttgcat aggcttctgc ttcaaaagct actctttcct
     1081 tatcttctct tgccttgatc gcatcctgaa ctgcatcttc tacctggatt ggaaaatttg
     1141 catcctgcaa gtttactttt gtaatgatta tccctgtgcc atattcatca attgtttgtt
     1201 gaatcagttg ttgagttaac aaagctattt ctgtcctgcc ttctgttagg ataaaatcca
     1261 aatcactttt accagcaact tcccgaatgg cactttcgct gacttcgcta agcgttacat
     1321 ctgcatccct aacattgaaa agataatcag tagggtcagc ttgtctatat tgcacaacta
     1381 aatcaacaac aatgatattc tcatcggaag tcaacattgt agtttgctgt ttaaatcgat
     1441 taacaacatt aacattaaca acttcaactc cttcaattgg ccatggaatg tgccatctca
     1501 aaccaggcat agttgtctcg ctatatgcac caaaccgaag cacaacacct cgttccgatt
     1561 gatcgatttg atacaagcct gatattaccc aaacaaacag tattaatatc acaataatag
     1621 attttggaag tcctcttggt ttttttcctg aaccggttga tttcatacca aacaatttat
     1681 tgaatctttt ttgccattcc tcgacgatct gatcaagatc tggtggctga tttttattgg
     1741 gatcccaggg agatttccca ttgccttgat cgttccatgc cataactgct taaactcctt
     1801 tatttatttc tctgtccttt cgctagttta ccttctatca gctgaatgtt tatat
//
LOCUS       DASCTH010000053         1777 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000074596, whole genome shotgun sequence.
ACCESSION   DASCTH010000053 DASCTH010000000
VERSION     DASCTH010000053.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000074596"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="QGH35_01100"
     CDS             complement(<1..313)
                     /locus_tag="QGH35_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HJL81163.1"
                     /translation="MHETITYEPDMYLTNKDVKDIQEIFQTPLIGTYNWDYTAADDRI
                     NRLYQLAKKRQWNVEIDLDWSQPWNQEDLTLEDYAYNHQMFNDYKPYIRLSDEKKLEF
                     QN"
     gene            complement(363..869)
                     /locus_tag="QGH35_01105"
     CDS             complement(363..869)
                     /locus_tag="QGH35_01105"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HJL81164.1"
                     /translation="MNKRIKAFTVLRWLSALLLLPINVLVIIPAILFDLEITDLNYWT
                     IWLSIAIGLPGLVLAMSCVRLFATLGGGGTPAPWDPIGNLIIAGSYCYVRNPMLSGVI
                     IMLFSEALFFRSLPFLLYAILFTVINMVYFPLIEEPGLIKRYGNTYKDYMKNVPRWIP
                     AAKPYNPE"
     gene            complement(853..1272)
                     /locus_tag="QGH35_01110"
     CDS             complement(853..1272)
                     /locus_tag="QGH35_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJL81165.1"
                     /translation="MNKELINAIRTIHSPNGWVHLSTIGLNGEPHVTPMMMGIHDHGL
                     LFSLTGKQKKRNLERDPRACVAISQSDVMNHVIVWGTIELRHDDLAQQIWNEMIRNAF
                     GKEYLGQIARKLSIEETSLGIMTPTRHRIYGFNEQED"
     gene            1414..1602
                     /locus_tag="QGH35_01115"
     CDS             1414..1602
                     /locus_tag="QGH35_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81166.1"
                     /translation="MKKNTILSLSSGIFFAVSLIHLIRFFSGGELSIFNHQLPQMVSI
                     LAAVVTVLLGWQLHSLKD"
BASE COUNT          524 a          329 c          355 g          569 t
ORIGIN      
        1 tattttgaaa ttctaacttt ttttcatccg ataatctgat atagggtttg tagtcattaa
       61 acatttgatg gttataggca tagtcttcca gagtaagatc ttcttgattc caaggctgag
      121 accaatcgag gtcaatttct acattccatt gtcttttttt tgccagttgg tagagtctat
      181 ttattcgatc gtcagcagca gtgtagtccc agttataagt cccgattagt ggagtttgga
      241 atatttcctg gatgtctttg acatctttat tcgtcaaata catatcaggt tcgtatgtaa
      301 tggtttcatg catctaatta ctcatcaatt caatacctta tttcattcgg tataattata
      361 cattattcag ggttgtaagg ttttgctgca ggaatccacc ggggcacatt tttcatataa
      421 tccttgtatg tatttccata gcgtttaatc aatcctggtt cttcaatcag ggggaagtag
      481 accatgttaa ttacggtaaa caagatagca tagagtaaaa agggcaggga tctaaagaat
      541 agtgcctctg agaacagcat gataatcact ccggataaca ttggatttct aacataacag
      601 tatgatccag caatgattaa gttaccaatg gggtcccagg gagccggcgt accaccacca
      661 cctagtgttg caaacaaacg gacacaactc atagccagca ctaacccagg taatcctatg
      721 gcaatggaaa gccagattgt ccaatagttg agatcagtga tctccagatc aaacaatatg
      781 gctggaataa taaccaaaac gttgattggt aacaaaagta gtgcagaaag ccaacgaaga
      841 actgtaaatg ctttaatcct cttgttcatt gaacccatag attcggtgcc ttgtaggcgt
      901 catgatccct aagctagtct cttcaatgga aagttttctg gctatttgac ccaggtattc
      961 tttgccaaag gcatttcgaa tcatttcatt ccaaatttgc tgtgccaaat cgtcatgtcg
     1021 gagctcaata gtgccccaga caattacatg attcatgaca tcgctttgac tgattgcaac
     1081 acaggctcgg ggatcacgtt ccaaatttct ttttttctgc tttccagtaa gactgaataa
     1141 cagtccatga tcatggatac ccatcatcat gggagtgacg tgaggttccc cgttaagccc
     1201 tatggttgat aaatgtaccc aaccgttggg tgaatgaata gttcgaatgg cattaattaa
     1261 ctctttgttc ataggttact attttaattt agccgatcat cagagtctaa acaagcattt
     1321 gagttttatt agatatgatt aatttccaat ggtaaatggt ttggaatctc aatagaattg
     1381 agaacattag gcaatattat tggtagtaat agtatgaaaa agaatacaat cttgtcgttg
     1441 agttccggga tcttttttgc agtctcactt attcatttaa tcagattttt ttcaggcggt
     1501 gagctcagta tttttaatca ccaactgccg caaatggtct ctatactggc agcggttgtt
     1561 actgtactct taggatggca attgcactct ctgaaggatt aatcgattca gttaaaaaaa
     1621 gatcgaatag gtggagtgaa gttctcagtg tcgaccagaa atgaatcgtg tccttggatg
     1681 gaatctaact gaatgaatgc agagtcaata ccattttctt gaaatgcttt gtagagatct
     1741 agttggcaat cgatcgggaa aatataatct gaattca
//
LOCUS       DASCTH010000054         2356 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000077532, whole genome shotgun sequence.
ACCESSION   DASCTH010000054 DASCTH010000000
VERSION     DASCTH010000054.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000077532"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="QGH35_01120"
     CDS             <1..1526
                     /locus_tag="QGH35_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012731.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HJL81167.1"
                     /translation="IRACYRKEKGNIIIDELPYQVSGNKLIEQIAAQMKDKKLPMVSD
                     INDESDHQTPIRVVITPKSSRVDHNKLMSHLFATTDLEKTIRVNMNLIGLNGKPKVYD
                     LKNLLLEWLEFRYQTVKKRLECRLSEVEERLHILEGLMIVYLNLDAVIRIVREEDKPK
                     EKLIKKFKLSEMQAESILNLRLRNLAKLEEEEIKIEKDELDTEQQELTKLLKSKAKIN
                     QLIQKEIVADAKEFGDVRRTILNEEAESSQALDETQLASSDPVTIVLSQSGWVRAAKG
                     TIDDPENLNYKSGDAYLTAVSGRSNQFAVFIDSTGRVYSIVANTLPSARSQGEPLSSR
                     LNPPDGATFAGVMSGTPETFWLLATNSGYGFFIKVGDLYTKNRKGKTCLRVPTGARVL
                     IPADTRHTDYPKKSLIAAANTAGKLLIFPTDQLLEMTKGKGLKVIGIPPKKFKTGSEA
                     LASVIVLSQEDSIRVYSGKKPKIIRPGDFDHFMSDRGKRGRSVPPFKKIDSIAVELK"
     gene            1550..2119
                     /gene="efp"
                     /locus_tag="QGH35_01125"
     CDS             1550..2119
                     /gene="efp"
                     /locus_tag="QGH35_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HJL81168.1"
                     /translation="MPNYSSRDFKPGLKILIGGDPYTILESQFSKPGKGQAFNKLRIR
                     NLKNGKVLEKTVKIGVNLEQADVTVREMQYLYNDGHQWHFMDVKSFEQIAASLEAIQD
                     SKQWLKEEYTCSVTLWNNQIILVEPPNFIETRVTKTDPGLKGDTAQGGVKPAQIETGA
                     SIKVPLFIDQGELIKVDTRTGQYVSRVKG"
     gene            complement(2144..>2356)
                     /locus_tag="QGH35_01130"
     CDS             complement(2144..>2356)
                     /locus_tag="QGH35_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014696.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HJL81169.1"
                     /translation="VSSITTVWKSEDLQAENPLTILKGEQMGQFNLGSTVILLVPKES
                     QLIWSETMKTGTRVKVGQVIAQINND"
BASE COUNT          809 a          416 c          502 g          629 t
ORIGIN      
        1 aaatcagggc ttgttataga aaagagaagg gcaatattat tattgatgag ttgccatacc
       61 aagtttctgg gaataagctc atcgagcaaa ttgccgccca aatgaaagat aaaaaattac
      121 caatggtgag cgacatcaac gatgaatccg atcatcaaac accaataaga gtggtgataa
      181 cacctaaatc ttcaagagta gatcacaaca aactgatgag ccatttattt gcaaccacgg
      241 atttggaaaa aacgattcga gtcaatatga atttaattgg attgaatggc aaaccaaagg
      301 tgtacgattt aaaaaatttg cttctagaat ggcttgagtt cagatatcag acagttaaaa
      361 aaagacttga atgccgctta agcgaagtcg aagaacgcct tcatatcctc gaaggattga
      421 tgattgttta tctcaacctt gatgcagtaa tacgtatcgt gagagaagaa gacaaaccta
      481 aagaaaaatt aatcaagaaa ttcaaattat ctgaaatgca agctgaatca attttaaatc
      541 tcaggctaag gaatctagcc aagttggagg aagaggaaat aaagattgaa aaggatgaac
      601 tagataccga gcaacaagaa ttaaccaaac ttctcaaaag taaggcaaaa attaatcaat
      661 tgatccaaaa agaaattgta gcggacgcaa aggagtttgg tgatgttcga agaacaattc
      721 ttaacgaaga agcggagagt tcccaggctt tggatgaaac ccaactggca tcaagtgatc
      781 cggtcacaat tgttttgtca cagagtggct gggttagagc tgcaaaaggc accattgatg
      841 atcctgagaa ccttaactat aaatcgggcg atgcttactt gactgcagtc tcaggaagaa
      901 gcaatcaatt tgctgttttc atcgacagta ctggcagagt gtatagtatt gttgcaaata
      961 ctcttccatc agcaagaagc caaggcgaac cattatctag tcgactcaat ccacccgatg
     1021 gtgcaacctt tgctggagtc atgtcgggaa caccagagac tttctggttg ctggcaacca
     1081 acagcggtta tggtttcttt attaaggttg gtgatttata tactaaaaac agaaaaggaa
     1141 aaacatgcct tcgggttcca acaggtgcac gtgttttaat tccggcagat acaaggcaca
     1201 cagattatcc taaaaaatct ctcatcgcag cggcaaacac tgctggtaaa ttgctgattt
     1261 ttccaaccga tcaactttta gagatgacta agggcaaagg gttaaaggtt attggtatac
     1321 cacctaagaa attcaagaca ggcagcgaag cattggcctc agttattgtt ttgagtcaag
     1381 aagattccat tcgtgtttat agtggcaaaa agccaaaaat tattagacca ggcgattttg
     1441 atcactttat gagcgatcga ggtaaaagag gccgttctgt tccaccgttt aaaaaaattg
     1501 actccatagc ggttgaactt aaataacaat tatctcccca agaccactta tgcctaatta
     1561 cagcagtcgt gattttaaac ccggtttgaa aatattgatc ggtggcgatc cgtacaccat
     1621 tctggaatct caattcagta agcctggtaa gggacaggct tttaataaat tgagaataag
     1681 aaatttaaaa aatggcaaag tgctagaaaa aacagtgaaa attggggtca atcttgagca
     1741 agcagatgtg acagtaagag aaatgcaata cctttataac gacggtcatc aatggcattt
     1801 tatggatgtg aagtcatttg aacaaattgc tgcatcgctc gaagcgattc aagattctaa
     1861 gcagtggttg aaagaagagt atacttgctc ggtaacgttg tggaataatc agattatttt
     1921 ggtagaacct cctaatttta tagagactag agtaactaaa acagatcctg gattgaaagg
     1981 ggatactgct cagggtggtg ttaaaccggc tcagattgaa actggtgcaa gcatcaaagt
     2041 gccactattt attgatcagg gtgaattaat aaaagttgat accaggacgg gtcaatacgt
     2101 ttcaagagtc aaaggataaa ttctcccgac aaacccagca gttttaatca ttattgatct
     2161 gagcaataac ttggcctact tttacacgag ttccagtttt catagtctca gaccatatta
     2221 actgagattc ttttggtacc agtaagataa ctgttgagcc taggttaaat tgacccattt
     2281 gctcaccttt gagaatagtt aatgggtttt cagcttgaag atcttctgat ttccaaacag
     2341 tggtgatgga agaaac
//
LOCUS       DASCTH010000055         2020 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000079780, whole genome shotgun sequence.
ACCESSION   DASCTH010000055 DASCTH010000000
VERSION     DASCTH010000055.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000079780"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(80..1336)
                     /locus_tag="QGH35_01135"
     CDS             complement(80..1336)
                     /locus_tag="QGH35_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HJL81170.1"
                     /translation="MGYYFQIGIGALILIILGVIFSNNIKNIKIKYLINAILLQFLIA
                     ALLTKVPFITSMFESISHGVLALKNATDQGTGFVFGYLADGTPKPFEVANPASANIFI
                     FSGLMLIMVVCVLAAIFWHWRILPIIIKFIAMFFKKPLNVGGPVGLSASANIIFGQVE
                     APLLIRPYLGQMTRHELLVLMTVGMSTISGGVMVVYTTMLSSLYGPTLIGHFLTASLI
                     SVPAAIMYANIMMPSNQKTEDQSAIEKSQLYRSTMDALTRGTQDGLQIVLSVAAILVV
                     LISLVALVNYSLEALPMVAGAPLSLERIAGWIFSPIAWCMGIPWAEAQLGGSLLGVKT
                     ILNEFVAYVDLAAIDASDLSERSRLIMLYALCGFANLSSVGILLSGMGAMIPERKDDL
                     IAVSGKALIAATLASCFTGLVVGAIS"
     gene            complement(1377..>2020)
                     /locus_tag="QGH35_01140"
     CDS             complement(1377..>2020)
                     /locus_tag="QGH35_01140"
                     /inference="COORDINATES: protein motif:HMM:NF015150.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HJL81171.1"
                     /translation="WQMGRELPENHPYKQFMFDNVYVSEIPEFKDKMQQLFKAYDLFA
                     KQLMQAIAIYLDLNQFYFDSAIDNGNSILRAIHYPPIESEDPGERSGAHGDINLITLL
                     VGGHRPGLEIFINEQWMPVKSERDTILCNVGDMLERFTNNHLPSVIHRVTTGVQKTTE
                     SARYSIPFFVHPNPDWLIETLPSCIDAEHPDLYPESILTDDFLQQRLYEIKLL"
BASE COUNT          636 a          424 c          397 g          563 t
ORIGIN      
        1 aacaaataga gataggaggc aatgagcgat atttatcttt gtgtcgactc cattttgacc
       61 aaggagttac gggtaaagtc taagagattg ctccgactac taggcctgta aaacaagagg
      121 caagtgtagc ggcaattaac gccttgccac tgacagcaat taaatcatct tttctctccg
      181 gtatcattgc acccattccg ctgagcaata taccgacact tgataggttt gcgaaaccac
      241 acagagcgta cagcatgatc agtctacttc gctctgacaa atcacttgca tcaattgcgg
      301 ctaaatctac ataggcaaca aactcattca gaatagtttt tacacccagt aatgatccac
      361 ccaattgggc ttcagcccaa ggtataccca tacaccatgc gatgggcgag aaaatccaac
      421 cagcaattct ctccagtgaa agaggtgctc cagcaaccat tggcaaagcc tcgagactat
      481 aatttacaag agcgacgaga gagattagta ccacaagtat agcggccaca ctgagaacta
      541 tctgcaagcc atcctgggtg cctcgggtta atgcatccat cgtacttcta tacaattgag
      601 atttttcaat agcactttga tcttctgttt tttgattact gggcatcatg atgtttgcat
      661 acatgattgc agccggaaca gaaatcagtg aagccgttaa gaaatgacca ataagggtag
      721 gcccataaag actgctaagc atggtcgtgt agaccaccat caccccacct gagatcgtag
      781 acattcctac tgtcatcaac accaacaatt catgtcttgt catttggccg agataaggtc
      841 tgatcaataa tggagcttct acctgtccaa aaataatatt ggctgatgca cttaacccaa
      901 ccggcccgcc cacattcagc ggtttcttaa aaaacatagc aataaatttt atgatgatag
      961 gtaatattct ccaatgccaa aaaatggctg ccagaacaca aactaccatg atcaacatca
     1021 agccagaaaa aatgaatata ttggcactgg cagggttggc aacctcaaaa ggtttgggag
     1081 ttccgtctgc aagatagcca aaaacaaatc cggttccttg atctgttgca ttttttagtg
     1141 ctagaactcc atgagaaatc gattcaaaca tagaggttat gaatggaact tttgtcaaca
     1201 gagctgcaat gaggaattgg agtagaattg cattgatcaa atatttgatt ttaatatttt
     1261 taatattgtt actaaatatc actcctaaga tgatcaggat caacgcccct ataccgattt
     1321 gaaaataata acccatgtga atcctattta tggaaataag aatctattct acaaatttat
     1381 aaaagtttta tctcgtataa acgctgttga agaaaatcat ccgttagaat gctttccgga
     1441 tagagatcag ggtgttctgc atcaatacaa gatggcaagg tctcgatgag ccagtctgga
     1501 ttggggtgga caaagaatgg tatcgagtaa cgagcactct ctgtagtctt ttgaacacct
     1561 gtcgtgactc tgtgaataac tgaaggtagg tgattatttg tgaatctttc tagcatatct
     1621 ccaacattgc acagaatggt gtcacgttca gattttacgg gcatccattg ttcattaata
     1681 aagatttcta aacctggtct atgaccccca accaagagag taattaaatt tatatctcca
     1741 tgtgcaccag atcgttctcc aggatcttcc gattctatgg gaggataatg aatggctctg
     1801 aggatgctat tgccgttatc tatggctgaa tcaaaataaa attgattcag atccagatat
     1861 atagctatgg cttgcatgag ttgcttggca aacagatcat atgccttgaa taactgttgc
     1921 atcttatctt tgaattcagg gatttcagag acatagacat tatcaaacat aaattgctta
     1981 taaggatgat tctcaggcag ctccctgccc atttgccaga
//
LOCUS       DASCTH010000056         2589 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000081184, whole genome shotgun sequence.
ACCESSION   DASCTH010000056 DASCTH010000000
VERSION     DASCTH010000056.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000081184"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            496..2043
                     /locus_tag="QGH35_01145"
     CDS             496..2043
                     /locus_tag="QGH35_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013086.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJL81172.1"
                     /translation="MNAFYEKIKLTKRELLINALKMLTLSSLPFSTRLALGNQHWDLI
                     IVGAGTAGLPTALFASQRGARVLLIEKAATIGGTLYLSTGQIAGAGTVFQEQKGIKDS
                     PEAHYQDIMRINHHTSDPELARLLVNHAGDTINFLHANGYRIKKDHPVTGIAHDHFTT
                     ARYIEGVQGGISILNVFKPMVETAIETGAITLLINSGVVDLVQARDGSVQGVIVEDQD
                     GLRSQYNGRNTVLASGGCAANPHLFEELHNVPLYVNAAYPYSQGTGLLLGQSTGGYLR
                     GGEKYASLLGGILSDENYPSPLYGHAPLYPPNRQPWEILVNTYGERFVREDHPSVDHI
                     EHGVVKQPGHRHWVIFDQRMLDESPPIIPGWSAAPAWSAERIMSESNQHTMFKSDHDL
                     EILAVKSGLNPKNLVATVERYNTDLINQKDDVFQRIHRPLPIIKPPFYSIRLQGWALC
                     SFAGLAVNGNLEVIKPSGEPVKNLYAVGEVIGAGTTTGNAYVNGMMVTPAITFGRLLG
                     EQILPLS"
     gene            complement(2036..2536)
                     /locus_tag="QGH35_01150"
     CDS             complement(2036..2536)
                     /locus_tag="QGH35_01150"
                     /inference="COORDINATES: protein motif:HMM:NF042461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HJL81173.1"
                     /translation="MKEFYKSAGLLIACIVLARFIMPANFTPLIAMAVFMPFITHNKK
                     LQLFLPVSLLLITDLALGFYGTTMLFVYGTMLLIGLLSRFLHKESIQALWINSILSVV
                     LWHGVVNFGVYMTGLGTQSLAQTYLLAIPFDLKLMASTLLFSSIFYGSRYLIKESSYF
                     GNRGLS"
BASE COUNT          826 a          528 c          514 g          721 t
ORIGIN      
        1 aatggacgcg aacattcatc aaaaattttc aaataatacg caacattcat gtgaccattg
       61 atatcacaca tgtctttagt gacgactatt tcaggagcat taaagggaaa tttttctttc
      121 ataaatcacc tagttgataa tttttcagta atataatccg tgagatgagg tgctaaaatg
      181 ctcatcagat aaaatattaa aaatgtcact aacaaagcaa tcgtaattgt aagcaatcta
      241 ttcagaaaat gcatataagt cctctattca cactgaagtt taggtttcct tataaatgat
      301 ggcaaacaaa accacaataa tacctactaa aaaaactaaa caagagcggt tttggttgtt
      361 gagacttatt gggtttcagt attcgttttg tgtgtgcatg atactttgtt atagcaaagt
      421 cataataact tcattggatt tttactactg tctcagacat cctttatacg atatgattga
      481 acgacaaggt gactagtgaa tgctttctat gaaaaaatca aattgaccaa aagagaactt
      541 ttgatcaatg ccttgaaaat gctcacattg agctcacttc ccttctctac ccgtctggca
      601 ttaggaaatc agcattggga tttaatcatc gttggtgctg gaactgcagg actacccaca
      661 gcattattcg cttctcaaag aggagctcgg gtacttttga ttgaaaaagc agccacaatt
      721 ggtggcaccc tctatttatc taccggacaa atcgctggtg ctggaaccgt gttccaagaa
      781 caaaaaggca ttaaggattc acctgaggct cattatcaag acatcatgcg catcaatcac
      841 catacctctg accccgaact ggcccgactg ttggtcaatc atgctggaga caccatcaat
      901 ttcctgcacg caaatggcta tcgcattaaa aaagaccacc ccgtaacagg tatcgcacat
      961 gaccacttta ctaccgctcg ctatatcgaa ggagttcaag ggggcatttc aatactcaat
     1021 gtgtttaaac ccatggtcga gacagccatt gagacaggtg ctattacact actgataaat
     1081 tctggtgttg tcgatttggt gcaagcaaga gatggttctg tgcaaggagt gattgttgaa
     1141 gaccaagatg gacttagaag tcagtataac ggacgaaata cggttttagc ctcaggggga
     1201 tgtgctgcca acccacattt atttgaagaa cttcacaatg tacctctgta tgtcaatgca
     1261 gcttatcctt atagtcaagg cacgggttta ttgcttggtc agtcgacagg aggatatctt
     1321 cgtggaggtg agaaatatgc atcactactc ggtggaattc tgtcagatga aaattatcca
     1381 tcaccattgt atggacatgc cccgttatat ccacccaata gacaaccttg ggaaatatta
     1441 gtcaacactt atggcgaacg ctttgtacga gaagatcatc ccagtgttga tcacatcgag
     1501 cacggagttg tgaaacaacc aggacacaga cactgggtaa tttttgatca aagaatgtta
     1561 gatgaatctc cccctattat tccaggttgg agtgctgctc cagcttggag cgctgagcga
     1621 atcatgagtg aatccaatca acataccatg tttaagagcg atcatgatct tgaaatattg
     1681 gctgttaagt cgggtctaaa tccaaaaaat cttgtagcca cggtggaacg gtacaacact
     1741 gacctaatca accagaaaga cgatgtgttt cagagaattc acagaccact accgatcata
     1801 aaaccgcctt tttattccat tcgattacaa ggatgggctc tatgctcgtt tgctggtctt
     1861 gccgtgaacg gtaatcttga agtgattaaa cccagtggtg aaccggttaa aaacctctac
     1921 gccgtcggcg aggtgattgg agctggaaca accacaggta atgcttacgt caatggtatg
     1981 atggtaaccc cggctattac ttttggccga ttacttggag aacaaatttt accgcttagc
     2041 tgagtcctct gttaccaaag tatgaagact ctttaatgag gtatcttgac ccatagaata
     2101 tactcgaaaa taataatgta ctagccatca atttaagatc aaaaggtata gccaaaaggt
     2161 atgtctgggc taaggattga gtacctaaac cagtcatata tacaccaaag ttcacaaccc
     2221 catgccacaa aacaacactt aatatagaat taatccataa cgcctggata ctctctttgt
     2281 ggagaaacct tgatagaaga ccgatcaaca gcatcgtgcc atatacaaac aacatcgtag
     2341 ttccgtaaaa ccctagtgcc agatctgtaa tgagtaataa actgacgggg aggaataatt
     2401 gtagtttttt attatgcgtt atgaacggca taaaaacagc cattgcaatc aaaggcgtaa
     2461 aattagcagg cattataaat cttgctaaaa ctatacaagc aatcaataga cctgccgact
     2521 tgtaaaattc tttcatagta atccttgttc gtgttgttta atgaggaagg cttaatgtac
     2581 caaatgaat
//
LOCUS       DASCTH010000057         1611 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000081983, whole genome shotgun sequence.
ACCESSION   DASCTH010000057 DASCTH010000000
VERSION     DASCTH010000057.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000081983"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /gene="ruvB"
                     /locus_tag="QGH35_01155"
     CDS             complement(<1..826)
                     /gene="ruvB"
                     /locus_tag="QGH35_01155"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HJL81174.1"
                     /translation="MSNIDSIISTETQEGEESIDRMLRPKSLAEYVGQEPVKEQMDIF
                     IKAAMNRKESLDHVLIFGPPGLGKTTLAHIIANELKVNLKHTSGPAIEKPGDLAAILT
                     NLEERDVLFVDEIHRLSPVVEEVLYPAMEDYQLDIIIGEGPSARSIRLDLTSFTMIGA
                     TTRTGMLTSPLRDRFGISQRLEFYNVEELTRIVERSAKILKVDIDQIGAQQIASRSRG
                     TPRIANRLLRRARDFAEVRAQGDINQKVANEAMELMDVDQHGLDVLDRKFLIAIIER"
     gene            complement(841..1437)
                     /gene="ruvA"
                     /locus_tag="QGH35_01160"
     CDS             complement(841..1437)
                     /gene="ruvA"
                     /locus_tag="QGH35_01160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018404379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HJL81175.1"
                     /translation="MIGSLNGLIRNKKPSEVLIEVNGVGYEVHVPLSTSFKLPAIDQR
                     THLLTHLIIREDQNTLYGFATEEERNLFRALIKISGVGPKLAITILSGTNVEDFVRSV
                     QAQDADALIHLPGIGKKTAERLLVEMKDRIDQIGGVNLTEGDGIEFSGIQVIKEAHNA
                     LSSLGYKSVEARKILEKIDSKGLTVEELLRQALQSLNK"
     gene            complement(1438..1602)
                     /locus_tag="QGH35_01165"
     CDS             complement(1438..1602)
                     /locus_tag="QGH35_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HJL81176.1"
                     /translation="MGSGAAEKEQVQYMVKIILGIKNKELKLDESDALAIAICHAHSY
                     SMDKKIGNDS"
BASE COUNT          435 a          375 c          264 g          537 t
ORIGIN      
        1 atctttcaat aattgctatt aaaaatttcc tatccaatac atccaaacca tgttgatcaa
       61 catccatcaa ttccattgcc tcattggcaa ccttttgatt aatatctccc tgtgccctaa
      121 cctccgcaaa atctcttgcc ctccttaata ggcggttagc aattcttggt gtccctctgg
      181 atcttgatgc aatttgctgg gctcctattt ggtcaatatc aacttttaaa atcttggcag
      241 aacgctccac aattctagtc aattcctcaa cgttataaaa ttctagtcgt tgtgatatac
      301 caaagcgatc tctaaggggt gatgtaagca tcccggtacg tgtggtcgct ccgatcattg
      361 taaatgatgt tagatccagt ctaattgatc tggccgaagg tccctctcct ataataatat
      421 caagttgata gtcctccata gcaggatata gaacttcttc aacaactgga ctcagtcgat
      481 ggatttcatc cacaaatagc acatctcttt cctctaaatt ggtcaaaata gcagcaaggt
      541 ctccaggttt ctcaatcgct ggacctgagg tatgttttaa attaaccttc aattcatttg
      601 caatgatatg tgctagagtt gtcttgccca accctggtgg accaaatatt aaaacatgat
      661 cgagggattc cttgcgattc atggcagctt taataaaaat atccatttgc tccttgacgg
      721 gctcctgacc gacatattca gccaagcttt taggccttag cattcgatcg atggactcct
      781 ctccttcttg ggtttcggtt gaaattatgc tgtctatatt cgacatggtt ttgaattcaa
      841 ttatttgttt aaagattgca atgcttgtct taaaagttcc tctacagtta atcctttcga
      901 gtcaattttt tccagaatct ttctcgcctc tacactttta tagcctaaag atgatagagc
      961 gttgtgtgct tctttgatta cctgaatgcc agaaaattct atgccatcgc cttctgttaa
     1021 gtttaccccg cctatctgat caattctatc cttcatctct accagcaaac gttctgccgt
     1081 cttttttcct atgccaggaa gatggattaa ggcatcagca tcttgggctt gaacacttct
     1141 aacaaagtct tcaacattag tgccagataa aatcgtaatg gctaattttg gacccacacc
     1201 acttattttt atcagagccc taaataaatt tctttcctcc tctgttgcga agccatacaa
     1261 ggtattttga tcttctcgaa taattaaatg cgtcagtaaa tgggtacgtt gatcaattgc
     1321 aggaagctta aaactagtcg atagtggaac atgaacttca taaccaacgc cattgacttc
     1381 tataagcacc tcggaaggtt tcttatttct gatcaaaccg tttagggaac caatcatcta
     1441 actatcgtta cctatctttt tatccattga ataactatgt gcatgacaga ttgctattgc
     1501 aagtgcatcc gattcatcga gcttcaactc cttatttttt atacctaaga tgattttaac
     1561 catatattgc acctgctcct tttccgcagc accagacccc acaactgctt g
//
LOCUS       DASCTH010000058        18452 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000083378, whole genome shotgun sequence.
ACCESSION   DASCTH010000058 DASCTH010000000
VERSION     DASCTH010000058.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 18452)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18452)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18452
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000083378"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="QGH35_01170"
     CDS             <1..858
                     /locus_tag="QGH35_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024852.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81177.1"
                     /translation="IERLIPRSLDAENNMLTPMEKPLIVVIGASGRTGRFVLEGLANK
                     DVHIRALSRNIERASADISGNYEWVQADVTQPETLVMAFQGSDIIISTIGAKPGKGPD
                     GPESVVYKGVINLVDEAKRAGAQHIIYMSSIGAGGAENFSTTFLNLFMNKTLKWKSLG
                     EEYMRNSGIDFTIIRPGGLRGEPGVSGIKFGQGDEIMGYIPREDVAEVMIESAFNLDA
                     VNKTFEIINDENLPVGAWRDEFKNLKTGEYGTIASGNLPVSYWVALFVLLILIVVLIR
                     RRRMRKKAA"
     gene            complement(855..2060)
                     /locus_tag="QGH35_01175"
     CDS             complement(855..2060)
                     /locus_tag="QGH35_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HJL81178.1"
                     /translation="MNATNNNKPLHGMRVIEMGQLIAGPFTGSVLGYYGAEIIKIEPP
                     TGDPVRYWRLTENNTSYWWHSVSRNKKSITLNLKTEEGRQIAKDLIMKSDVLIENFKP
                     GKLEEWGMGPDDLKKDNPGLITARISGYGQTGPRSHLPGYASVCEGYGGFRYVNGFAD
                     RPPVRPNLSIGDTLAGLHAAMGVMLAYIQREKDPNNKGQVVDTAIYESVFNLMEAVVP
                     EYSGCGAIREPSGSTITGIAPTNTYLTLDKKHVIIGGNGDSIFKRLMIAIGEPNMAED
                     PRFKHNEGRVKHEKEIDDAILEWSSKHTSEEILSVMDEASVPAGPIYSVEDMMKDEHY
                     IARGMFQEVEANGRTLQIPAMIPLLTETPGSTEWSGPKLSEHTHEVLSELLGKNDQEI
                     KKLVDEGIV"
     gene            complement(2089..2964)
                     /locus_tag="QGH35_01180"
     CDS             complement(2089..2964)
                     /locus_tag="QGH35_01180"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HJL81179.1"
                     /translation="MTAFASNKKRISVNGKRLAYTESGSGDPIIFLHGNPTSSYLWRN
                     ITPYLESQGRCVCIDLIGMGDSDKLDNPDENSYRFEEHYHYVSAAIESLTNGKNITFV
                     LHDWGSALGFNWCYHNPESVKGITYMEAIVKEMTWEDWQDEAKGIFQGFRSDAGESLV
                     LEKNYFVERVLPGSIIRKLSDEEMEEYRRPFTNEGEDRRPTLSWPREIPINNYPENVC
                     AIVNQYAEWMQTNDLPKLFINAEPGAITTGRIRDFCRSWKNQTETTVKGIHFIQEDVP
                     DEIGNAISSWYKNIE"
     gene            complement(2961..3800)
                     /locus_tag="QGH35_01185"
     CDS             complement(2961..3800)
                     /locus_tag="QGH35_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HJL81180.1"
                     /translation="MKTTASEFINEPHKAITLMGMSGIGKTTLANKLPKANWFHYSGD
                     YRIGTKYLEEPILDNIKERAMEVPFLRTLLKTDSIYISSNITVDNLAPISSFLGKIGA
                     PSKGGLTLKEFSIRQELHKSAEINAMKDVPDFIEKSSRIYGYDHFINDAGGSICELID
                     SEAMDALVNHTVILYIEENDEVRQTLIERSKNHPKPLYYNHDFLVNNLEVYQKETGGD
                     HESMDPDEFVRWIFPRLLDYRKAKYQAIAKQYGYTVQASDTMNLNNENELIDLVAKAI
                     ESE"
     gene            4004..6460
                     /gene="thrA"
                     /locus_tag="QGH35_01190"
     CDS             4004..6460
                     /gene="thrA"
                     /locus_tag="QGH35_01190"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF007003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HJL81181.1"
                     /translation="MVKEIWQVYKFGGSSLADGQCLLTVNRIIHQNKSSNLIVVVSAM
                     AGMTDQLVDYSKSKDNSILLKIEDRYRRTLQETIHEKSIIKSLEDEFRNDLNTIKELA
                     QSFSTEKISIEENQVLGFGELWSSRLIQGILSQASSSDLNDRDLHLINPFDVITLYPT
                     DMGANVDWAESEKSFNKVFSGRNGIFVMAGFIAKNSDSVPTNLGRNGSDYSASIIGSL
                     AKARSVSIWTDTDGIMTADPNQIQTAKTIEQMSYDEAIELAYFGAEVIHEKTMLPLMN
                     KGIPIYIRNTFNPESQGTEISSKVNGVNSVKGITTIDNIILVNVEGSGMIGVPGTAKR
                     LFSCLSDAGISVILITQASSEHSICFAIDDKYSDQIESVLQVEFNIDFQNGNLQQLQI
                     QENCSIIAVVGSGMTGTKGIAAQFFKAITQSEVNIMAIAQGSSEKNISVVVKKDDLNQ
                     AVQSVHNAFFTGKTELIIALVGFGNVGQEFYHQVEAQKDKIFKDFNLEINFVAIANSS
                     HMILDEDISESDIASLIAKDQSCQASKVEDIIDFLEKTPADLKVFIDCSASDTIPDLY
                     TECFQSGINVITANKKGLSGPLSRYRDIMRSAKNNKRTFLYETTAGAALPFIKSVKDI
                     SISGDVIEEIEGVFSGTLAYLFNSFDGSKPFSNIVSTAREQGYTEPDPRDDLSGMDVA
                     RKLAILAREMGLNIEVKDVEIENLVSQNHSDLSVVDYLKEMVNDDSIMEKRYKQANHD
                     GKVLCYLARLNADGKASVKLEALSKEHPFSRLEGTENIIKYKTRRYSKHPLVHRGPGA
                     GPEVTAAGIFGDLLAFLNTH"
     gene            6480..7787
                     /gene="thrC"
                     /locus_tag="QGH35_01195"
     CDS             6480..7787
                     /gene="thrC"
                     /locus_tag="QGH35_01195"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HJL81182.1"
                     /translation="MRYISTKGHSIPSIEDALFSGTSTDGGLVMPESIPEVKINDFSQ
                     FLSYQEFSHQILLPYFAGSSVENELEDIVNKAFNFPLINKDYFEKDLQYSVLELFHGP
                     TAAFKDFGARFLSLTMSEITRKNKNPGITTILVATSGDTGGAVASAFDQCPEFKVVVL
                     FPEGRVSPRQKHQLTCWGDNVTSIEVKGEFDDCQRLVKEAFSNNELSMKHNLCSANSI
                     NIGRLLPQATYYAWSSLKRYKGSGDNTSFIVPTGNLGNAFACFIAKEMGFPIDQIIFA
                     TNSNKTISDFISTGQWEPRATLPTLASAMDVGNPSNMERFFHFFDSPETLIDKLQAVS
                     VTDEQIIDQIRSEYQDNQILCCPHTATALHVYRGLCDSDKKETHWCIAATAHPSKFEN
                     IVEPIIGEEIMVPEKLRALLELDSSFVTIDPELNQLVDIIASA"
     gene            complement(7771..8571)
                     /locus_tag="QGH35_01200"
     CDS             complement(7771..8571)
                     /locus_tag="QGH35_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HJL81183.1"
                     /translation="MATQLIDIGANLTHESYDLDIDQVIKRSQDAGIAKIIVTASSIS
                     ESKRVAAMIKQYSGILWGTSGVHPHHAKEADDLFAETLDELIQNENIVAVGECGLDYF
                     RNFSEPREQQKVFRIQLQIALKNSLPIFLHQRDAHNDFIGILKEHESSVIDGVAHCFT
                     GNKKQLKAYLDIGLYIGITGWLCDPRRNQDLIDSIHYIPMDKLLIETDSPYLMPKALE
                     KELKTRRNEPCFLPYIAQAIAELKKLDLQEVMLNTYQNSERLFFKQKQ"
     gene            8625..9392
                     /locus_tag="QGH35_01205"
     CDS             8625..9392
                     /locus_tag="QGH35_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81184.1"
                     /translation="MKLYKQKLLLLFLVLLVSPPDSNSIETDSNLIIKQSYEDYLGEH
                     KDDLIRSYQSLLSKLAESENESVVSLLTLHEFLIADISNRKDKKKFSKSCIDRNQIIQ
                     QSNRYPPEVYAFLSACYGLSAQSNLLKAASHGAKSGRMIERALEIDSNNPFIYLVKGI
                     GDYQRPELFGGSSENAIKNLNRAIDLYQMRLENTMQIGEAIAHYHLAVIYFFTKQEFE
                     LSLKHARLALKLEPNYRRAMTFENQLMTIVKETELIN"
     gene            complement(9395..10414)
                     /locus_tag="QGH35_01210"
     CDS             complement(9395..10414)
                     /locus_tag="QGH35_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002708304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJL81185.1"
                     /translation="MIARILAVFHARNLEFVRDRGTLSWNIMFPVIVLFGLNFVFSAG
                     ERNQFKIGVLTNNPVISEIDHPFMDIRYINKIQIVDQDAGLESVRRHKLDMLVDLDGP
                     VIHYWINEESPTGYMVEKILLQSSKDEFLKGVVQGKNIRYVEWLLPGVLGMNLMFGCL
                     FGVGYVIVRYRKNGFLKRLKATPLQAIEFIIAQVLSRLILTMSMAIFIYIGASFILET
                     RMEGSYFVLLLVGILGATSLISMGLLIASRVSSEELAGGLLNLMTWPMMTLSGIFFSL
                     EGSPEWVITLSNFFPLTHTLNAARAIMIDGATITEISFELMILIVISVVFTSLGAFLF
                     KWQPD"
     gene            complement(10411..11268)
                     /locus_tag="QGH35_01215"
     CDS             complement(10411..11268)
                     /locus_tag="QGH35_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJL81186.1"
                     /translation="MRKKYPDVIAVDGISFAVKKGICFGLLGPNGAGKTTVIEIIEGI
                     TASDGGTILYKDQPINKNFHKETGIQFQATALQEFLTVKETLKLFHKLYPHPAHLDEI
                     IQECSLENLLSRDNRKLSGGQRQRLLVAIALINQPELLFMDEPTTGLDPQARRNFWNM
                     VEKIKLRKTTIILTTHYMEEADYLCDEIIIMDKGKILASGEPKKLIAAHFKEVILELP
                     ENEVVNGELDIPIKYELKYGHYEIYTRDISSTLDLLKKHNISLDNLKIRSFNLEDLFL
                     ELTGSELRS"
     gene            complement(11319..13931)
                     /gene="acnA"
                     /locus_tag="QGH35_01220"
     CDS             complement(11319..13931)
                     /gene="acnA"
                     /locus_tag="QGH35_01220"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HJL81187.1"
                     /translation="MTKSLQSFEINGIKYFYNDLNTVEKSKLATIPYSHKILLENVLR
                     HHDTDDSHSEDADAIINWNPKSKTTTEISFTPSRVILQDFTGVPAVVDLAAMRDAVSE
                     LGGNPNDINPLSPAELVIDHSVQVDHFGTQNALDQNNKIEFERNKERYKFLKWGQESF
                     SNFKVIPPNTGIIHQVNLEYLGRVIFSEQREHGRVAFPDTLVGTDSHTTMINGIGTLG
                     WGVGGIEAEAAMLGQPITMLIPDVVGIKVCGRLEDGVTATDLVLTVTELMRTHGVVGK
                     FVEFFGDDLANLPVSDRATLSNMAPEFGSTCAIFPIDKETIRYLHLTGRDSHHIELVE
                     KYAKTQNLWRESGQPEANYKDIIELHLENIQPCISGPKRPHDRINLDTVPTVIEKELG
                     NANRQCDELSHGSVLIAAITSCTNTSNPAVMIAAGLLARNARKLGLKPKPWVKTSLAP
                     GSRVVTEYLKKTNLLEDLESLGFNLVGYGCTTCIGNSGPLNVEVAKTVSSEALITSSV
                     LSGNRNFEGRIHPLVKLNFLASPPLVVAYAIAGSIMINLSKEPLGTLENGTEIFLRDI
                     WPAQQEIQQLITETITPEMFNQSYTTAIDGDSNWQNITTPKGEIFDWDQDSTYIKKPP
                     YFDSMTMKPEPRTKIKQARVLALLGDFVTTDHISPAGSIDPSSPAGQYLLERGVEKKD
                     FNSYGSRRGNHEVMMRGTFANIRLRNKLAAATEGSWTKLSPGTEPISIYEAASHYQQN
                     QTELIVIAGKEYGTGSSRDWAAKGTALLGVKAVIAESFERIHRSNLIGMGVLPLEFNT
                     GENADQLQLDGFELFDIENIHPDSKHIKITATNNEQETSKEFITKVRIDTPKEWDYFL
                     HGGILQYVIRRLVT"
     gene            complement(14000..15100)
                     /gene="mnmA"
                     /locus_tag="QGH35_01225"
     CDS             complement(14000..15100)
                     /gene="mnmA"
                     /locus_tag="QGH35_01225"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007017776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HJL81188.1"
                     /translation="MNNLIMVGMSGGVDSAVSASMLIEQGFEVEALHMTNWEDDEPFC
                     TAAQDFQDTRQVCRELGITLHHVNFSKEYKEKVFDQTLKEFDMGLTPNPDVLCNRMIK
                     FDDFTNHAFRLGAKKIATGHYARIKKDQQRLSLLKGLDREKDQSYFLHSINPEIMNSI
                     LFPIGEMTKKEVREYANRKGLPNYNKPDSTGICFIGERPFKDFLKTFLPPKPGVIISE
                     EGEELGSHEGLMYFTIGQRKDLGIGGIKNKPDQPWYVAKKDMENNRLVVVQGREHPIL
                     WTQKVFVEKVRWLHSEPIKNLEIEKSFHCFAKNRYRQSDVQCKITPNRKGLEVDFKEP
                     QWAITYGQYMVFYEQDECLGGGKIMPQPNASN"
     gene            complement(15124..16488)
                     /gene="purB"
                     /locus_tag="QGH35_01230"
     CDS             complement(15124..16488)
                     /gene="purB"
                     /locus_tag="QGH35_01230"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010957828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HJL81189.1"
                     /translation="MKKHQLQAISPIDGRYAEKCAHLRDLFSEASLIKYRVITEIKWL
                     QFLAQEADIKDIKMMPNEVSQGLQKIIDEFSIEEALQVKEIESEINHDVKAVEYYLQD
                     KLSSLPNSKDYIAFIHFGCTSEDINNISYALMIQNGVKAIRISLEEITSSLRSFSHQW
                     HDLAMLAHTHGQPATPTTMGKEFANFLYRLEQQFDLLDRLKYKAKMNGAVGNFNAHKI
                     AYPNIDWPKVSENFLQTLNLTNNPYTTQIEPHDWIAELFHCMIRMNNILLNFSKDLWL
                     YISMGYFEQLMNSNEVGSSTMPHKINPIDFENAEGNLGIANALLTHLSSKLSNSRMQR
                     DLSDSTALRNIGSCFGHTEIACSSLKRGLGKIRVNEAKMKQDLDAHWEIIAEAIQTIL
                     RREHIDNAYEQLKNLTRGEDFNKNHFEQFIQSLDVSKSVQQELLDLSPNNYLGFASIL
                     AKKI"
     gene            16588..17967
                     /locus_tag="QGH35_01235"
     CDS             16588..17967
                     /locus_tag="QGH35_01235"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018719526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HJL81190.1"
                     /translation="MTDKDYRLEKDSMGELKVPSEKHWGAQTQRALENFKISNTKMPS
                     EFIQSLALLKWAISAANCDLGLLSSDKSQAIGKIALEIAEGEFSDEFPVDVFQTGSGT
                     STNMNMNEVIANLGSSEGMPIHPNDDVNMGQSSNDVIPSAINVASTLLIKNSLLGSLS
                     HLQKTLEDKSSSLSGIVKTGRTHLMDAMPITFGQEIGAWASQIEAAKRQFEATLPTMQ
                     HLAIGGTAVGTGVNSHPDLSEKVSKLLNQKTGLNFQPSTDLFKAISAQDGSLELSSNA
                     RNLAVALIKIANDLRWMNSGPLAGLGEISLPPLQPGSSIMPGKVNPVIPESVLMVAAQ
                     VIGNDATIVLAAQSGNFQLNVMLPVIAFNLIQSVSLLSHACMALADKAIKDFEVNQEN
                     IDAVINKNPILVTALNDTIGYEQGAIIIKKAYQEKRTIIDVALEMTELDESELKELLN
                     PSNLTNNKL"
     gene            17995..>18452
                     /locus_tag="QGH35_01240"
     CDS             17995..>18452
                     /locus_tag="QGH35_01240"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HJL81191.1"
                     /translation="MLENIRQKFQDSHLFDDPVTNVMNRLPQEIVDNYLQKTDLKHAG
                     VLIGLEENAKSGLNVLLTKRTTKLKQHAGEISLPGGTYDQQKDQDILSTALREAHEEI
                     GLDKDQAEILGFLEPQVSLGTGFIVTPLIASIESNFVPKIDANEVAELFSV"
BASE COUNT         5526 a         3492 c         3532 g         5902 t
ORIGIN      
        1 attgagagac tcattccaag atcacttgat gcagagaaca atatgctcac accgatggaa
       61 aaaccattga tagtagtcat cggagcatca ggcaggacgg gtaggtttgt tcttgagggt
      121 ctagctaaca aagatgttca catccgtgca ctatccagaa acattgaaag agcaagtgca
      181 gatattagtg ggaattatga gtgggtccaa gctgatgtga cccagccaga gactttagtt
      241 atggcatttc aaggttccga tattattatc tcaacgatag gagctaaacc tggaaaggga
      301 ccagatggac cagaatctgt ggtttacaaa ggggttataa atttagtcga tgaagcaaaa
      361 agagcagggg ctcaacatat tatttatatg tcctccattg gagccggcgg tgctgaaaat
      421 ttcagtacaa ctttcttgaa tctatttatg aataaaacct tgaaatggaa gtctttgggc
      481 gaagagtata tgcgcaatag tggaatagat tttaccatca tcagaccggg tggtttacga
      541 ggtgaacctg gtgtgagtgg gattaaattt ggtcaaggtg atgaaatcat gggatatatt
      601 cctcgagagg atgttgccga ggttatgatt gaatctgcct ttaatctaga tgcagtcaac
      661 aaaactttcg agatcattaa cgatgaaaac cttcctgtgg gagcctggag agacgagttt
      721 aagaatttga agaccggaga atatgggacg attgcatctg gcaacttgcc cgtttcttac
      781 tgggtagcat tgttcgtttt attaatctta atcgttgttt tgattcgtag gagaagaatg
      841 agaaaaaagg ctgcctaaac gataccttca tcaactaatt tcttgatttc ttgatcattt
      901 tttcctaaga gttccgataa cacttcatgg gtgtgttcac tcagttttgg ccctgaccat
      961 tcagtacttc ctggtgtttc tgttaataaa ggaatcattg ccggtatctg cagtgttctg
     1021 ccattggcct cgacttcttg gaacatccct ctggcaatat aatgttcatc tttcatcata
     1081 tcttcaacac tgtagattgg acctgcgggt acggaggctt catccatcac agataagatt
     1141 tcctcagaag tatgtttaga tgaccactct agtattgcat catcaatttc tttttcatgc
     1201 tttacacggc cttcattatg tttaaatcta gggtcttctg ccatgttggg ttcgccgatg
     1261 gctatcatca gtcgtttaaa aatagaatcc ccattaccac caataataac gtgtttttta
     1321 tccaaggtta gatatgtatt ggtaggtgct ataccagtga ttgtagatcc agatggttct
     1381 ctgattgcgc cacatccaga gtattctggc accacagctt ccatgagatt gaaaaccgat
     1441 tcataaatag ctgtatctac aacctggcct ttattattag gatctttttc cctttggatg
     1501 taggccaaca taactcccat ggcagcatgc aagccagcta gagtatcccc gatactcaag
     1561 ttgggtctga caggaggcct atctgcaaag ccattaacgt atctgaatcc accatatcct
     1621 tcacacactg aagcataacc tggaagatga gaacgaggac ctgtttgtcc atagcctgat
     1681 attcttgcag taattaaacc agggttgtct ttttttaaat catcaggtcc catcccccat
     1741 tcttctaatt tcccaggttt aaaattctct attaaaacat ccgatttcat aattaaatct
     1801 ttggctattt gtctaccttc ttccgttttt aaatttaatg taattgattt tttatttcga
     1861 gaaacactat gccaccaata ggatgtgtta ttttcagtga gccgccaata tcgaactgga
     1921 tctcctgtgg gaggttcaat ttttattatt tctgctccat agtaaccaag aacgctccct
     1981 gtaaaaggac cagcaatcag ctgtcccatt tcaatgactc tcatgccatg gagtggttta
     2041 ttgttattag ttgcattcat tgtttacatc aatattactg acagtattct attcaatatt
     2101 cttataccat gatgatatag catttccaat ttcatccggt acatcttcct ggatgaagtg
     2161 aatgcctttg accgtagttt ctgtttgatt cttccaactt ctacaaaaat ctctaattct
     2221 ccctgtggta attgcaccag gttctgcatt gatgaacaac ttgggtaggt catttgtttg
     2281 catccattca gcatattgat taacaattgc acagacattt tcaggatagt tgtttatagg
     2341 tatttctctc ggccatgaca atgtgggtct tctgtcttca ccctcattag taaaaggtct
     2401 tctgtattct tccatttctt catcacttag ctttctgatg attgaaccgg gaagtactct
     2461 ttcaacaaag taattttttt ctaacaccaa actttctcca gcatcagacc taaaaccttg
     2521 aaaaatacct ttggcttcat cctgccaatc ctcccaagtc atttctttta caattgcttc
     2581 catataggta attcccttaa ccgactcagg gttgtgataa caccaattaa aacccaatgc
     2641 tgaaccccag tcgtggagaa caaaagtgat attcttacca ttggttagag attctatagc
     2701 agcactaaca taatgataat gttcttcaaa tcgataggag ttctcatcag gattgtctag
     2761 tttatccgag tctcccatcc caatcagatc aatacaaaca caccgaccct gagattctaa
     2821 atagggagta atatttcgcc agagatagga agaagtcggg tttccatgga gaaaaataat
     2881 aggatctcca gatccagatt ctgtataggc taagcgctta ccattgacgg aaattctttt
     2941 cttgtttgat gcaaaagcag tcattccgat tcaatagctt ttgcgacaag atcgatcaat
     3001 tcattttcat tattgagatt catagtatct gatgcttgaa cagtgtaacc gtattgtttt
     3061 gcaatggcct gatactttgc ttttcggtaa tcaagcagcc ttggaaaaat ccaacgaaca
     3121 aactcatctg gatccattga ttcatgatct ccacccgttt ctttctgata gacctctaaa
     3181 ttatttacta ggaaatcgtg attataatag agaggttttg gatgattttt tgatcgttca
     3241 attaatgttt gcctcacttc gtcattttct tcaatgtata gaatgactgt atggtttact
     3301 aatgcatcca ttgcttcgct atcgatcagt tcacagatgc tgccaccggc atcattgata
     3361 aaatgatcat agccataaat tctactggat ttttcaataa aatcgggaac atccttcata
     3421 gcattaattt ctgcactttt atggagttct tgtcttattg agaactcttt tagtgttaat
     3481 ccccccttgg atggagcgcc aatttttcct agaaaagatg atattggagc gaggttatca
     3541 actgtgatat tgctagaaat atagatagag tcagtcttga gcagtgttct caaaaaagga
     3601 acttccatcg ctctttcttt gatattatct agaataggtt cttctaggta ttttgttcca
     3661 attcgatagt ctccagagta atgaaaccaa ttagctttgg ggagtttgtt tgcaagtgtt
     3721 gttttgccaa tacctgacat tcccattaag gttatcgctt tatgaggctc attaatgaat
     3781 tccgatgcag ttgttttcat ggttgtattg ttctggcaat taaactatct agcaataact
     3841 gtttaatcga ttgttctatt tcatattgtc attttgatat aaaagactca atatagccat
     3901 atttacagat tggttctgtt tgtatgtaaa tgtgttgtga aaagagtatc atggtcaact
     3961 ttttaatggc ataaaaatat tagtactgag ggtatataaa aagatggtta aagagatttg
     4021 gcaagtttat aaatttggtg ggagcagttt agcagatggg caatgtctgc taacagtcaa
     4081 ccgaattatc catcaaaata agagctctaa tcttattgtt gtggtttctg caatggcagg
     4141 gatgaccgat caactcgtgg attactctaa atcaaaagat aattcaattt tgctcaaaat
     4201 agaagatcga taccgaagga cacttcaaga aaccattcat gaaaaatcaa ttataaaaag
     4261 ccttgaagat gagtttagga atgatttgaa taccataaag gaacttgcac aatcattttc
     4321 aacagaaaag ataagtatag aagaaaatca agtgctaggt tttggtgaac tatggtcctc
     4381 caggcttatt caaggtattt taagtcaagc atcttcaagc gatctaaatg acagagattt
     4441 gcatctgatt aatccttttg atgttattac tctttatcct acagatatgg gtgccaatgt
     4501 agattgggca gagtcagaga aatcttttaa taaagtattc tctggtagga atggaatatt
     4561 tgttatggca ggctttattg caaaaaattc agattctgta cccacgaatt taggtagaaa
     4621 tggcagcgat tattcagcat caatcatagg ttcactggct aaggctaggt ccgtttctat
     4681 ctggacggat actgacggca ttatgacagc tgatcctaat caaattcaaa cagcaaaaac
     4741 cattgagcaa atgtcttatg acgaagcaat agagttagct tattttggag ctgaggtaat
     4801 acatgaaaaa acaatgctac cattgatgaa taaagggatc cctatttaca ttcggaacac
     4861 attcaaccct gaatctcaag gaactgaaat ttcatctaag gtcaatggtg ttaatagcgt
     4921 taaagggata acaaccattg acaatattat tttggtaaat gttgaaggct cagggatgat
     4981 aggtgtccca ggaacagcaa agcgattatt ttcatgtctt agcgatgctg gaatatcagt
     5041 catactcata acacaagcta gttcagaaca ttcaatttgt tttgctatag atgacaagta
     5101 ttcagatcaa attgaaagcg ttcttcaagt ggaatttaac atcgatttcc aaaatggaaa
     5161 tctacaacag ctccaaatac aagaaaattg cagcattatt gctgtagttg gatcaggaat
     5221 gacgggaaca aaaggtatcg cagctcaatt ttttaaagca attactcaat cagaagttaa
     5281 tataatggct attgcacagg gctcttcaga aaaaaatatt tctgtagtgg taaaaaagga
     5341 cgatttaaat caagcagttc aatccgttca caatgccttt tttacaggca aaactgaact
     5401 aattatagct ctagttggtt ttggtaatgt gggacaagaa ttttatcatc aagtggaagc
     5461 acaaaaagac aaaattttta aagattttaa cttggagatt aattttgtgg ctatcgcaaa
     5521 ttcatcccat atgattctag acgaagatat ttccgagtct gatatagctt cattaattgc
     5581 aaaagatcaa tcatgtcaag catctaaagt tgaagatatt attgattttc tagagaaaac
     5641 acctgcagat cttaaggttt ttattgattg ttcagcaagt gatacgattc cagatctcta
     5701 tacggagtgt ttccaatcag gaataaatgt cattacggca aataagaagg gtttaagcgg
     5761 acctttaagt cgttataggg atattatgag atctgctaaa aacaataaac gtactttttt
     5821 atatgaaacc actgcaggag ctgctctacc gtttataaaa tcagtaaaag atatttcaat
     5881 ctcaggagat gtaatcgagg aaatagaagg agtgttttca gggacattgg cctatttatt
     5941 caattctttc gatggatcta aaccattttc aaatatcgtc tcaactgcca gagaacaggg
     6001 ttatacagag ccagatccta gagatgatct gtcaggaatg gatgtcgcaa gaaaattagc
     6061 aattttagca agagaaatgg gactaaacat tgaagtcaag gatgttgaaa tagaaaatct
     6121 agttagtcag aatcatagtg atcttagtgt tgtagattat ttaaaagaaa tggtaaatga
     6181 tgattcaata atggagaaaa gatataaaca agccaatcat gacgggaaag ttctttgcta
     6241 cctagctcgt ctaaacgctg atggcaaggc ctcagtaaaa ttggaagctt tgagtaaaga
     6301 gcatccattt tctcgattag agggtacaga aaatatcata aaatacaaaa ctaggcgtta
     6361 ttcaaaacac cctctagtgc atcgcgggcc aggggcagga ccagaagtaa ctgctgctgg
     6421 tatatttggt gatttattag cttttttgaa cactcattag cctagaaaat taaccaataa
     6481 tgagatacat cagtacaaag ggccattcta tcccctcgat agaagatgct ttgttcagtg
     6541 gcacttctac agacgggggc ctggtaatgc ctgagtcaat cccagaagtt aagattaatg
     6601 actttagtca gtttttgagt taccaggaat tttctcatca aatattatta ccttattttg
     6661 caggctcaag tgtagaaaat gaattagaag atatcgttaa caaagctttt aattttccat
     6721 taattaacaa agattatttt gaaaaagatc ttcagtactc tgttcttgaa ttgttccatg
     6781 gaccaactgc agcatttaaa gactttgggg cgcgtttctt atctttgaca atgtctgaga
     6841 taactagaaa aaataaaaac cctggaataa ccactatttt ggttgccaca tcaggagata
     6901 ccggaggtgc tgttgcatca gcttttgatc aatgccctga atttaaagtg gtggttttgt
     6961 ttccagaagg aagagtctct ccaagacaaa agcatcagtt aacttgctgg ggtgataatg
     7021 tcacttcaat tgaggttaaa ggagagtttg acgactgtca gcggttggtt aaagaagctt
     7081 ttagtaacaa tgaactctca atgaagcata atttatgttc tgccaatagt attaatattg
     7141 gtcgtttgct tcctcaagca acttattacg catggtctag cttaaagcga tataaaggat
     7201 ctggagataa tacgagtttt attgtgccga caggtaattt aggtaatgct tttgcctgtt
     7261 ttattgccaa agagatgggg tttccaatag atcagattat ttttgcaacc aattcgaata
     7321 aaaccatttc cgattttatt tccacaggcc aatgggaacc aagagcgaca ctacctacgt
     7381 tagcctctgc aatggatgtt ggcaatccaa gtaatatgga aagattcttt catttttttg
     7441 atagtcctga gaccttgatt gataaacttc aagccgtatc ggtaaccgac gaacagatca
     7501 tcgaccaaat cagaagtgag tatcaagata atcaaatact gtgttgtcct catactgcaa
     7561 ccgctttgca tgtctatcga ggactttgtg atagtgataa aaaagagacc cattggtgta
     7621 ttgctgctac agcacaccca tcaaaattcg aaaacatagt cgaaccaatc attggcgaag
     7681 agattatggt gcctgagaag cttagagctc ttcttgaact tgattcatcc tttgtaacaa
     7741 tagacccaga actgaatcaa ttggttgata ttattgcttc tgcttaaaga aaagcctctc
     7801 tgagttttga taagtgttta acatcacttc ttggagatct aattttttta actcagcaat
     7861 cgcttgggct atatagggta aaaaacatgg ttcatttctc cttgttttta attccttctc
     7921 caaggcctta ggcataaggt agggtgagtc ggtttcaatt aataacttat ccatgggaat
     7981 atagtgaatt gaatcaatca gatcttggtt tcttcgtgga tcacaaagcc agccggtaat
     8041 gccaatatac aatccaatat ccaggtatgc tttcagttgt tttttatttc cggtgaaaca
     8101 atgagctaca ccatcaatga ccgaagactc atgctctttt aaaatgccaa taaagtcatt
     8161 atgtgcatct ctttgatgga gaaaaatggg gagagaattt ttaagagcaa tttgaagttg
     8221 gatccggaag actttttgtt gctctctagg ttctgaaaag tttctaaaat agtccaagcc
     8281 acattctcct acggccacaa tattttcgtt ttgtattaac tcatcaagtg tctcagcgaa
     8341 taaatcgtct gcttctttgg catggtgagg atgtacccct gatgttcccc atagaatccc
     8401 agagtattgc tttatcattg cagccaccct ttttgattca ctgatagaac ttgctgtgac
     8461 tataattttt gctatgccag catcctgact tctcttgatg acttggtcaa tatcaagatc
     8521 atagctttca tgagttagat tggctccgat atcgattaat tgagttgcca taaatttttc
     8581 tataatttat tgatacaatg ccagtgcatc aaatttgaag agttatgaaa ttgtataaac
     8641 aaaaactact gttgctcttt ttagttcttt tggtcagccc gcctgattcg aattcaattg
     8701 aaacagactc taaccttata atcaaacaaa gctatgaaga ctatcttgga gaacataaag
     8761 atgatttaat tcgtagttat caatctctct tgagcaaact cgctgagagt gaaaatgaga
     8821 gcgttgtttc tctgctgaca ttacatgaat ttttaattgc agatatctcc aacagaaaag
     8881 acaaaaaaaa attttcaaaa tcttgcattg atcgaaatca aatcatacaa caatcgaatc
     8941 gttatcctcc tgaagtgtac gcatttcttt ctgcttgcta tggtttaagc gctcaaagta
     9001 acttattaaa ggccgctagt catggtgcta aatctggtcg gatgattgag cgggctcttg
     9061 aaatcgactc aaacaatccc tttatatact tggtaaaggg gataggggac tatcaaagac
     9121 ccgaactatt tggtggcagc tctgaaaatg caatcaagaa cctcaatcga gccatagatt
     9181 tataccaaat gcgtctggag aacaccatgc agataggaga agctatagct cattatcatc
     9241 ttgcagtgat ttattttttt acaaagcaag aatttgagct ctctttaaag catgctcgat
     9301 tagccttaaa actagagccc aattatagaa gggcaatgac ttttgaaaac caattaatga
     9361 cgatcgtcaa agaaactgaa cttattaatt gatcttagtc gggttgccat ttaaacaaaa
     9421 aggcacctag tgatgtaaat acaacagaga tcactattag aatcataagt tcaaaagaaa
     9481 tctcggtgat ggttgcccca tcaatcatga ttgctcttgc cgcattcagt gtgtgtgtca
     9541 gagggaaaaa attggaaaga gttattaccc actccggtga tccttccaag gagaaaaata
     9601 tcccagataa agtcatcatc ggccaggtca taagattgag taatcctccg gctagttctt
     9661 cactcgagac tctcgaagca atcagcaaac ccatggaaat gagtgaagta gctcccagta
     9721 tcccaaccag cagcaataca aagtaactgc cttccattct tgtttcaaga ataaaactag
     9781 cccctatata aataaaaatt gccatagaca ttgtcaggat taatcgagac aggacttgag
     9841 caataatgaa ttctattgct tgtagtggcg ttgctttaag ccgtttaagg aaaccattct
     9901 ttctataacg cacaatgaca tagcccacac caaataaaca gccaaacatt aaattcatac
     9961 caagcacccc aggcaggagc cattcaacgt aacggatgtt ttttccttgc acaacacctt
    10021 taaggaattc atcttttgag gattgtagaa ggattttctc aaccatatag cccgttggtg
    10081 actcttcatt tatccagtaa tgaatgactg gaccatctag gtcaacaagc atgtctaatt
    10141 tatgccttct aactgattcc aatccagcat cttgatcaac tatttggatt ttattaatgt
    10201 atcggatatc catgaacgga tgatcaattt cactgatgac tggattgttg gttaggactc
    10261 caattttgaa ctggtttcgt tctcctgcag aaaagacaaa attgagacca aaaagcacta
    10321 tcactggaaa cattatattc catgacaatg tacccctatc ccttacaaat tcaaggtttc
    10381 ttgcatgaaa gactgccaaa atcctagcta tcatgagcgg agttcgctcc cagtgagttc
    10441 gagaaataaa tcttccaaat taaaggatct aattttcaag ttatcaaggg aaatattatg
    10501 tttttttaag agatccaaag tgctcgaaat gtctcgagta tatatttcat aatgcccata
    10561 tttgagttca tattttatag gtatatctaa ctcaccattc accacttcat tttctggtag
    10621 ttccaaaata acttctttaa aatgagcagc tattaatttt tttggttcac cactggctaa
    10681 aattttaccc ttgtccatga tgataatttc atcgcagagg taatcggctt cttccatata
    10741 atgcgtagtc agtattatgg tcgtttttct taatttgatt ttctccacca tgttccaaaa
    10801 attacgccta gcttgaggat caagacctgt tgttggttca tccataaata ataattcagg
    10861 ttggttgatc agagcaatgg caaccagtaa tctctgtctt tgacctcctg ataatttcct
    10921 attgtctcta gacagaaggt tttctagaga acattcctga atgatttcat ccagatgagc
    10981 tggatgagga tacaatttat gaaacaattt caatgtttct ttaactgtca agaattcttg
    11041 taaggccgtc gcttgaaatt gaatgcctgt ttccttgtga aagttcttat taatgggctg
    11101 atctttatat aggatagttc ctccatcaga cgcagtaatg ccttcaataa tttctataac
    11161 agttgttttc ccagctccat tgggtccaag taaaccaaag catatccctt tttttacagc
    11221 aaaactaatc ccgtcgacag cgatcacgtc cgggtatttt ttcctcaaat ttttaacttc
    11281 aagtatgttt aacatgtatc aagcttagaa tttttagttt aagtaactaa gcgcctaata
    11341 acgtattgca agatgccacc atgtagaaag taatcccatt cctttggggt atcaattcga
    11401 actttagtaa tgaattcttt actggtttct tgttcattgt tggtggcagt tattttaatg
    11461 tgtttactgt caggatgaat attttctata tcaaataact cgaaaccatc taattggagt
    11521 tgatctgcat tttcaccagt attgaattcc aggggcaaca cacccattcc aatcaggttg
    11581 gatcggtgga tacgttcaaa tgattccgca atcacagctt taacacctag gagtgcagtt
    11641 cctttggctg cccaatccct tgatgatccg gtaccatatt ctttccctgc aatgactata
    11701 agttcagttt ggttttgttg gtagtgcgat gcagcctcgt aaattgaaat aggctctgtt
    11761 cctggagata gtttggtcca actcccttca gttgcagctg ccaatttatt tctcagtcta
    11821 atatttgcaa aagtacccct catcatcact tcatgattgc ctcttctgga gccataggaa
    11881 ttaaaatcct ttttttccac tcctcgttcc aataaatact gtcctgcagg tgagctagga
    11941 tcaatactgc cagcaggaga gatatggtct gtggttacaa agtctcctaa cagagccagt
    12001 actcttgctt gtttaatttt tgttcttggt tcaggtttca tggtcatgga atcaaaataa
    12061 ggaggtttct tgatgtaagt tgagtcttga tcccagtcaa aaatttctcc ctttggtgta
    12121 gttatgttct gccaattcga atcaccatca atggctgttg tataactttg attaaacatt
    12181 tccggggtta tagtttccgt tatcaattgt tgaatctctt gttgtgctgg ccaaatatca
    12241 cggaggaaga tttctgttcc gttttccagt gttccaaggg gctctttgct cagattgatc
    12301 atgatggagc ctgctatagc ataggcaaca accaagggtg gtgatgccag aaaattaagc
    12361 tttaccaaag gatgaatacg accctcaaaa tttctatttc ccgacaagac actggaagta
    12421 atgagtgctt ctgatgagac agttttagca acttctacat tcagtggacc tgagttacca
    12481 atgcaggtgg tacagccata gccgacaagg ttaaaaccga gcgactcaag gtcttcaaga
    12541 agattagttt ttttgagata ttcagtgaca actcttgaac ctggagctag gctggttttc
    12601 acccaaggtt ttggctttag tcctaatttt ctggcatttc ttgctaacag gccagcagca
    12661 atcatgactg ctggatttga tgtattggtg caactggtga tagcagcaat caacacagat
    12721 ccatgactca attcatcgca ttgtctgttg gcattgccaa gctctttctc aataactgtt
    12781 ggcactgtat ccagattgat acgatcatga ggcctttttg gtcctgaaat acaaggttgg
    12841 atattttcta agtgcagttc aataatatct ttatagttag cttctggttg tccactttcc
    12901 cgccacagat tctgagtttt agcatacttt tcaaccaact ctatgtgatg actatctctt
    12961 ccggttaagt gtaaatagcg gatggtttct ttatcgattg gaaaaatggc acaagtactt
    13021 ccgaattcag gagccatatt actgagtgtg gctctgtctg aaactggaag attcgcaaga
    13081 tcatcaccaa agaactcaac aaattttccg actactccat gtgttctcat tagttcagta
    13141 acagtaagga ccaaatctgt tgcggtgact ccatcctcaa gtcgaccaca gaccttgatg
    13201 ccaactacat cgggtatcag catagtgatg ggctgtccca gcattgcagc ctctgcttct
    13261 ataccaccca ctccccaacc cagtgttcca attccattaa tcatggttgt atgagagtct
    13321 gtaccaacta aagtatctgg gaaagcaacc cttccatgct ctctttgttc tgagaaaata
    13381 acacgaccca aatactcaag attgacttga tgaatgatac ctgtgttggg tggtatgact
    13441 ttaaagttag agaaggattc ttgtccccat tttagaaatt tgtaacgttc tttgtttctt
    13501 tcaaactcaa ttttattatt ttgatccaag gcattttgtg taccaaaatg atccacttga
    13561 actgaatgat ctatgactaa ttctgctgga gagagagggt tgatatcatt ggggtttcct
    13621 cctaattcag aaactgcatc tctcatagcg gcgagatcaa cgacagctgg cactccagtg
    13681 aaatcttgca ggatgactcg actcggagtg aacgatattt ctgttgttgt ttttgatttt
    13741 ggattccaat taataattgc atctgcatcc tctgaatggg aatcatcagt gtcatggtgt
    13801 ctcagaacat tttccaaaag gattttgtga gagtagggga ttgtagctag ttttgatttt
    13861 tcaaccgtat ttagatcgtt gtaaaaatac ttgatgccat tgatttcaaa cgattgaagc
    13921 gatttagtca taaaaatagt ccattaatct tttctctaat cttgggcggt gattgtatct
    13981 gaatacttcc tttttgtctt tagttcgatg cattgggttg aggcataatt ttacctccac
    14041 ctaaacattc atcttgttcg taaaacacca tgtattgtcc ataagtaatc gcccattgtg
    14101 gttctttaaa atcaacttcc aacccttttc gattgggcgt tattttgcat tgtacgtccg
    14161 attgacgata ccggtttttt gcaaaacagt ggaagctttt ttctatttcc agatttttta
    14221 taggctcaga gtgtaaccaa cggacttttt caacaaatac cttttgggtc cataaaatgg
    14281 gatgctctct tccttggaca actaccaggc ggttattttc catgtctttt ttagcaacat
    14341 accatggctg atctggcttg ttcttaattc caccaatccc aagatctttt cgttggccga
    14401 tcgtgaaata cattaaccct tcatgactcc caagctcttc accttcttcg gatataatca
    14461 cccctggttt tggaggcaga aaagttttta aaaagtcttt aaaaggcctc tcaccaatga
    14521 aacaaattcc agtgctgtct ggtttgttgt aattaggtag tccttttcta tttgcgtact
    14581 ctctgacttc tttttttgtc atttccccaa tcgggaaaag tattgagttc ataatttctg
    14641 gattaatcga gtgtaggaaa taactttggt ccttttcccg atccagtcct tttaatagac
    14701 ttaatctttg ctgatctttt ttgattcgtg cgtaatgtcc tgttgctatt ttttttgcac
    14761 ccagtctgaa tgcatggttg gtaaagtcat caaatttaat cattctattg caaagcacat
    14821 cagggtttgg tgttaacccc atatcaaatt cttttaatgt ttgatcaaaa actttttctt
    14881 tgtattcttt agaaaaattc acatgatgca atgttatacc caactctcta cagacctgcc
    14941 ttgtatcttg aaaatcttga gctgctgtgc agaagggctc gtcatcttcc caattggtca
    15001 tgtgtagggc ttctacttca aaaccttgct cgatcagcat agaagcagat acagcagaat
    15061 cgacaccacc cgacattcct accatgatta aattgttcac tcccatgttg aattacttgg
    15121 gaactatatt ttttttgcca gtattgatgc aaacccaaga tagttgtttg gagacagatc
    15181 gagcaattct tgttgaactg attttgaaac atctaacgat tggataaatt gttcgaaatg
    15241 atttttatta aaatcttctc cacgagttaa gtttttgagt tgttcatagg cattgtcaat
    15301 atgttctcga cgtaaaattg tctgaatggc ttcagcaata atttcccagt gagcatcaag
    15361 atcctgtttc attttggctt cattgactct aatttttccc aaacctcttt tcagtgagct
    15421 acaggcaatt tctgtgtgcc caaaacaaga acctatgttt ctaagagcgg tagaatcact
    15481 caaatctctt tgcattcttg aattagatag tttggaacta agatgggtca ataacgcatt
    15541 agcaattccc aaatttcctt ctgcattttc gaaatcaatg ggattgattt tatgtggcat
    15601 ggttgaggag cccacttcat tgctattcat tagttgttca aaataaccca ttgaaatgta
    15661 aagccagaga tctttgctga aattcaataa aatattattc attcttatca tgcaatggaa
    15721 taattcagcg atccagtcat gaggctcaat ttgggtggta taaggattgt ttgttaagtt
    15781 taaggtttgc aagaaatttt ctgaaacttt tggccaatca atatttgggt aagcaatttt
    15841 atgagcatta aaattcccaa cagcaccatt cattttggcc ttatatttca gtctatccag
    15901 taagtcgaat tgttgttcaa gacgatagag aaaattagcg aattctttac ccattgtggt
    15961 tggtgttgcg ggttgtccgt gggtatgggc tagcattgcc agatcgtgcc attggtgcga
    16021 gaaagatctt agtgatgatg taatctcttc aagggagatg cggatggcct tcaccccatt
    16081 ctgaatcatc aaagcatagg agatattgtt gatgtcttca gatgtacagc caaaatgaat
    16141 gaatgcaata tagtctttag aattaggcag tgacgacaat ttatcttgca ggtagtattc
    16201 aactgctttg acgtcgtgat tgatttcaga ctcaatttct ttgacctgta gagcctcctc
    16261 tatagagaat tcatcaataa tcttttgcaa tccctgactg acctcattag gcatcatttt
    16321 aatgtctttt atgtctgctt cttgagccaa aaactgaagc cattttattt cagtaataac
    16381 tcgatatttg atcagagatg cttcgctgaa gagatccctt aaatgagcac atttttctgc
    16441 gtacctccca tctattggag agattgcctg cagttgatgt tttttcatga aattcacctg
    16501 tacttacaaa agtgaatgat acactaatct tactttcttg accgccattg tgtgtgtatt
    16561 atcgtatatg gatatgtgga gtacaaaatg actgataaag attatcgcct agaaaaagac
    16621 agtatggggg agcttaaagt accgagcgaa aaacactggg gggcacaaac acagcgtgct
    16681 ttagaaaact ttaaaataag taatactaag atgccgtctg aattcattca atcattggct
    16741 ttactaaaat gggctatatc agctgccaat tgtgatctgg gtttactttc aagtgataaa
    16801 tcacaagcca ttggtaaaat tgcactagaa attgcagaag gcgaattttc tgatgagttt
    16861 ccagttgatg tttttcaaac aggatctgga accagtacga atatgaatat gaatgaggtg
    16921 attgctaatc taggctcctc tgaaggcatg ccaatacatc ctaatgatga tgtcaatatg
    16981 ggacaaagta gtaatgatgt gatcccatcg gcaatcaacg tggcatcaac acttttgata
    17041 aaaaatagtt tgcttggttc tctgtcacac ctacaaaaaa cccttgaaga taaatcctca
    17101 agcttatccg gtattgtcaa aacaggcaga actcatctaa tggatgccat gcctataact
    17161 tttggtcaag aaataggggc atgggcgagc cagatagagg cagcaaaaag acaatttgaa
    17221 gcaactttac caacgatgca acacctagca atagggggaa ctgctgttgg cacaggcgtt
    17281 aactctcatc ctgacttatc ggagaaagtt tcaaaactac tcaatcaaaa aacaggatta
    17341 aactttcaac catcgaccga tttattcaag gccataagtg ctcaggatgg atcattggaa
    17401 ttgtcatcca atgctcgaaa tcttgcagtt gccttaataa aaattgccaa tgatctgcgt
    17461 tggatgaata gtggaccgct tgctggctta ggtgaaatat cactcccacc gttgcaacca
    17521 ggaagtagta ttatgcctgg gaaagtgaat cctgttatac ctgaatctgt tttgatggtt
    17581 gcagcccagg tcatagggaa tgacgcaact attgtcctcg ctgcacagtc aggtaatttt
    17641 cagttgaacg tgatgttacc ggtgattgca ttcaatctta ttcagtctgt ttcacttcta
    17701 agtcatgcgt gtatggcgct agcagacaaa gctataaagg actttgaggt caatcaagaa
    17761 aatatagacg cagtgatcaa caagaatcca atattagtca cagcattaaa cgatacgatt
    17821 ggttatgaac agggtgcgat catcattaag aaagcttatc aggaaaaaag aaccattatt
    17881 gatgttgctc tggagatgac cgaattggat gagagtgaat tgaaagaatt attgaaccca
    17941 tccaatttaa cgaataataa gctttgaaat ctgaaaaaac catccctaac gattgtgctt
    18001 gaaaacatca gacaaaaatt tcaagatagc catctgtttg atgatccagt caccaatgta
    18061 atgaatagat tgcctcagga aattgttgat aattatctgc aaaaaactga tctgaagcac
    18121 gcgggtgttt tgataggatt agaagaaaat gcaaaatcgg gcttaaatgt tttactcact
    18181 aaaagaacaa caaaactcaa acaacatgcc ggtgagatca gcctaccagg aggcacctat
    18241 gatcaacaaa aagaccaaga tattcttagt acggctttga gagaagccca tgaggaaatt
    18301 ggtcttgata aagaccaagc tgaaatatta ggttttcttg agccacaagt cagtcttgga
    18361 accggtttca ttgttacgcc tttaattgca agcattgaga gcaattttgt acctaaaata
    18421 gatgccaatg aagttgcaga acttttttct gt
//
LOCUS       DASCTH010000059         3588 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000084030, whole genome shotgun sequence.
ACCESSION   DASCTH010000059 DASCTH010000000
VERSION     DASCTH010000059.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000084030"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="QGH35_01245"
     CDS             complement(<1..680)
                     /locus_tag="QGH35_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024809.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HJL81192.1"
                     /translation="MSEKYLIFGAELSPYSIKVRSYFRYKNIPHTWIVRDQTTQKEYN
                     KYAKIQIVPLVITPENEGLQDSTPIIQLMEKQHPDNTIVPKEPHTAFVSRLLEEYADE
                     WMVKCMFHYRWRYPEDQISAANRFAELFTPTWINRIPIANAVFKKYAAATFRKRQKSR
                     LWVVGSNENTEGLIEKSFSDFLQLLETHLQTRPYCFGFRPSIGDFGLWGQLYNAWTDP
                     TANKIIESG"
     gene            complement(680..1963)
                     /locus_tag="QGH35_01250"
     CDS             complement(680..1963)
                     /locus_tag="QGH35_01250"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HJL81193.1"
                     /translation="MRKVIHPFIFLTLVANTLIALGTEISVAPVVDETRRQIERIPRN
                     DIWWTVNGEDMLWNFKNLNKLFPTNTIYRNGPVKPLKTKIYPEIGALQIQTPSGNKTF
                     VDFLESDESTAIAVVILHKGNIVFEHYPRMNPHEKPVYWSVTKVFVSTLVAMLEAEKK
                     IDIDKPVDFYLPELIGSDYEGILIKNILDMATGIKCSEEYIDKSSCYYQYSMAVGDGM
                     WDESSPNNPYEYIAELNVGRYGEQGKQFDYSGVNTFVLGWLVEKITGMQLQDALSEMI
                     WTHIGAESDAAIFAPRYGVPVMHGGLLARPRDVARFGLLFTPSHQIVSDEQLVSDKHI
                     HTLMYEGRPELMSNQGIPMDRAGWAIKHNIYQWDMVFKNNDIYKRGWAGQGLIVNPEK
                     DTVAVWNGYFKDDNQSEIKLTPIIRRALDLIGSDK"
     gene            complement(1972..3270)
                     /locus_tag="QGH35_01255"
     CDS             complement(1972..3270)
                     /locus_tag="QGH35_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008490613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81194.1"
                     /translation="MIRSIFVLSALLTTNLFAADLKKDLDIELASLMEKVVEWRHDIH
                     QNPELSNREFNTSKKVANHLESLGIKTETGIAYTGVVGLIEGGKSGPTIALRADMDAL
                     PVEEKTGLSYASTARASYLGNDVGVMHACGHDAHVAVLMGVAEFLAKNKKHLKGKVLL
                     IFQPAEEGPPAGEGGGAKMMLAEGLFEKYQPEVIFGMHVGNAPHGYVFVKSGPAMAAA
                     SAYEINIKGVQSHGSRPWEGLDPIMAAAQLINALNTVVSRRINIINNPAVVSVGIVKA
                     GTRNNIIPEEAQIIGTIRTFDQNLRAEIYDEIRQIANGIAAGTGTQVTVEFDVGGFYP
                     VTVNNPELLDRMESSFKAATNGKFVELDEPRTGAEDFSYFAQEIPGLYFSLGVNNKGV
                     TEPQPGNHSPYFTIDDDALDEGLKSFIYLTLDYPGANEAK"
     gene            complement(3315..>3588)
                     /locus_tag="QGH35_01260"
     CDS             complement(3315..>3588)
                     /locus_tag="QGH35_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HJL81195.1"
                     /translation="VHGVSEREKQSDNPFDWNTVCNNIEKELAKDGFKRSVDYEIMLV
                     PNIVNITYGRGVGYNLEEEIFDKSTHAISATRIRKQLRKAGKLKDK"
BASE COUNT          968 a          808 c          657 g         1155 t
ORIGIN      
        1 cccgattcaa taattttatt tgctgttggg tcagtccaag cgttgtataa ctgcccccac
       61 agcccaaaat cgccaattga tggccgaaaa ccaaaacaat aaggtcgagt ttgaaggtgc
      121 gtttcaagca actgcaaaaa gtcgctaaaa gatttttcaa ttaagccctc tgtgttttca
      181 ttagagccaa caacccatag cctagacttt tgtcgctttc taaaagtggc cgcagcgtat
      241 tttttaaaca ctgcgttggc aatggggatt ctattgatcc aagtgggtgt aaaaagctca
      301 gcaaaacggt tggctgcaga aatctggtcc tctgggtatc gccaacgata gtggaacatg
      361 cactttacca tccattcatc tgcatactcc tctaataatc ttgaaacaaa agctgtatgg
      421 ggctctttag gcacaatggt attgtctggg tgttgttttt ccatgagctg aatgatcggt
      481 gtagagtctt gtaatccctc attctctggt gttatcacta gagggacgat ttgtattttt
      541 gcgtatttgt tatattcctt ttgggttgtt tgatctctaa caatccaggt atgtggaatg
      601 tttttatatc taaaataaga ccgaaccttt attgaataag gggacagttc tgccccaaat
      661 ataaggtatt tctcagacat tacttatcag aaccaattaa atcgagcgct cttcgaatga
      721 tcggagtcag tttaatttcg ctttgattat catctttaaa ataaccattc catactgcaa
      781 cagtatcctt ctcggggttt acaattaagc cctgcccggc ccatcccctt ttgtaaatat
      841 cattgttttt aaaaaccatg tcccactggt aaatattgtg cttgattgcc cagcctgccc
      901 tatccattgg aatgccttgg ttgctcatca gctcaggcct gccttcatac atcaaggtat
      961 gaatatgctt gtcagaaacc aactgttcat ctgaaacaat ttgatggctg ggtgtaaaca
     1021 ataatccgaa ccgagcaaca tcccggggtc tagccaaaag cccaccatgc atgaccggaa
     1081 ccccatacct aggtgcaaat attgctgcat cactttcggc gccaatatgg gtccaaatca
     1141 tttcactgag agcatcctgt agctgcatgc cggtgatttt ttcaaccaac catccaagca
     1201 caaaggtgtt tactccagaa taatcaaatt gtttcccttg ttcgccatat cggcccacat
     1261 tgagctcagc aatatattca tatggattat taggtgagct ttcatcccac ataccatcac
     1321 caactgccat ggagtattgg tagtaacagg agctcttatc tatatattct tcggaacatt
     1381 tgatgccagt cgccatatct aatatatttt ttatcaaaat cccttcataa tcagagccga
     1441 ttaactctgg caagtaaaaa tccactggtt tatcaatatc aatttttttc tccgcttcta
     1501 acatagccac caatgttgaa acaaacactt tggtcacaga ccaatacact ggtttttcat
     1561 gagggttcat tcttgggtag tgttcaaata caatatttcc tttatgaagt atcacaacag
     1621 caattgctgt tgactcatcg ctttctaaaa aatctacaaa tgttttgttg ccagatggtg
     1681 tttgaatttg taaagcgcca atctcgggat aaattttggt ttttagtggc tttaccggac
     1741 cgtttctata gatagtgttt gttggaaata acttattaag gtttttaaaa ttccaaagca
     1801 tgtcttcgcc attaaccgtc caccaaatgt cattccttgg gattcgttcg atttgtcttc
     1861 tggtctcatc aacaacgggt gcaacagata tctctgtgcc caatgcaatc agtgtattgg
     1921 ctacaagagt taaaaaaata aaggggtgta taacttttct cacaataacc tctattttgc
     1981 ttcgtttgct cccggatagt ctagggtaag atagataaaa gactttaaac cctcatccaa
     2041 cgcatcatca tcaatggtaa aatagggcga atgattcccc ggctgcggtt ctgtaactcc
     2101 tttattgtta accccaagac taaaatagag accaggtatt tcttgtgcga aatatgagaa
     2161 atcttctgcc ccggttctgg gctcatccag ctctacaaac ttaccattgg tcgctgcttt
     2221 aaaggagctt tccatccggt ctaataactc tgggttatta acagtaacag gataaaaacc
     2281 gccaacatca aattcaacag tgacttgtgt gcctgtgcca gcagctattc cgttagctat
     2341 ttgtctgatt tcatcgtaaa tctctgcacg aagattttga tcaaatgttc taatggttcc
     2401 aattatttgt gcctcctctg gaatgatgtt atttctagtg cccgccttta ctatcccaac
     2461 agaaaccaca gcagggttgt tgataatatt aattcttctg ctgaccactg tgtttaatgc
     2521 attgattaat tgtgcagcgg ccataattgg atcaagccct tcccaaggtc ttgacccatg
     2581 ggactggaca ccttttatat taatctcata agccgatgca gcagccatag caggtcctga
     2641 tttaacaaaa acataaccat ggggggcatt acccacatgc attccaaaaa ttacttctgg
     2701 ttgatacttt tcaaacaaac cctccgctaa catcattttt gctccgccgc cttcgcctgc
     2761 aggagggcct tcttctgcgg gttgaaaaat taatagaacc ttgcctttta agtgtttttt
     2821 gtttttagcc aaaaactctg caacacccat taataccgca acatgcgcat catggccgca
     2881 tgcgtgcatg acccctacat cattcccaag atagctagca cgggccgtgg aggcataaga
     2941 aagtcctgtt ttttcttcga ctggcaatgc gtccatatct gctcttaatg caatcgttgg
     3001 gccacttttg ccgccttcta ttagacccac aacaccggtg taggcaatgc ctgtttctgt
     3061 tttaatgcca agcgactcaa gatggtttgc aacttttttt gaggtgttaa attccctatt
     3121 actaagctcg gggttttgat gaatgtcatg tctccactca accactttct ccattaaaga
     3181 tgcgagctca atatcgagat ccttttttag atctgctgca aatagattgg tggttagcaa
     3241 ggcagaaaga acaaaaatac tcctaatcat tggttagctc cattatttat tggttgttcg
     3301 ggctgtgtta ttgtttattt atcttttagt ttgcctgctt ttcttaattg cttgcgtatc
     3361 ctggttgcac taattgcgtg agtgcttttg tcaaatatct cttcttccaa gttgtatccc
     3421 acacctctac cataagtgat gtttactata ttaggaacca acataatttc ataatcaacg
     3481 ctccttttaa aaccatcctt tgctagctct ttttctatat tattacaaac cgtattccag
     3541 tcgaatggat tgtctgattg tttttccctt tcagatacac catgcaca
//
LOCUS       DASCTH010000060         5887 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000084065, whole genome shotgun sequence.
ACCESSION   DASCTH010000060 DASCTH010000000
VERSION     DASCTH010000060.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000084065"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="QGH35_01265"
     CDS             complement(<1..364)
                     /locus_tag="QGH35_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HJL81196.1"
                     /translation="MNVLVIAEHNDGVINVSTSKTLSCVSDLSSQQTDLLVLGNNSEL
                     ATQAALFQNVNSVLYLEDQRFDHQVAAVMSPVIAHLATEYDCVLCPSTTFGRDLMPRV
                     AALLDVGQMTDIMAVTGPL"
     gene            complement(382..1134)
                     /locus_tag="QGH35_01270"
     CDS             complement(382..1134)
                     /locus_tag="QGH35_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018152403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HJL81197.1"
                     /translation="MNKILVCIKRVIDYNIKIRINSDESGVVTEGVKMSINPFDEIAL
                     EEALRIKESGKANEIIVVSIGSEDCQQQLRTGLAMGADRAILVEHQEQAEPLIVAKLL
                     YEIVQQEKPELILLGKQAIDDDCSQTGQMLAELCSYAQATFISKLDIEGNVLTVTREV
                     DQGLESLEIDLPAIVTTDLRLNEPRYVKLPDIMKAKKKPLDVMNSSEFNVSTDAFFKV
                     IKVSAPPPRQSGIMLTNADEAFDALKTKGFFD"
     gene            1273..1770
                     /locus_tag="QGH35_01275"
     CDS             1273..1770
                     /locus_tag="QGH35_01275"
                     /inference="COORDINATES: protein motif:HMM:NF039797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HJL81198.1"
                     /translation="MVIFLQCLLVLSGCTQEEQLKNERSTSTGYNVRLGSSCFNKDTI
                     QDYRVLDRSNLVIYGRPKSRSYHLQITPPASIRSSDSIYFNSRTGRICGFAGDELIIP
                     DSIFSERHSIIAVNELDDIAHYNLLLRFGIGDPLEVVEPDKETSPEITRELSETDNQE
                     NDNNE"
     gene            1733..2497
                     /locus_tag="QGH35_01280"
     CDS             1733..2497
                     /locus_tag="QGH35_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81199.1"
                     /translation="MKPTTKKMTTMNKESILQKDHQTVAGKIIYLSEKEDRQGQERGR
                     EYFIINKHANGHRKIVAHCEIDDRPAVMRDITYSLDENWLPLDCFVRISVNDQFMGSG
                     WFNFGDDYAECEANTLLDGRNSQKMQTKGRLKTFQNHAIACDAWHLRLYDRSKKGPQN
                     IGEMLLSSPDHRGATGPMLFSITSTIDFLGEESVTIKAGTFDALHFQFVGTPGLPQEH
                     PPYDIWCTNDEDYLFLKGSVGGYMQTYYELVELKSS"
     gene            complement(2523..3371)
                     /locus_tag="QGH35_01285"
     CDS             complement(2523..3371)
                     /locus_tag="QGH35_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013080851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HJL81200.1"
                     /translation="MTYSCFEYELRDHIAHISMSRGDKFNTMTKAFWSELPELVDKIS
                     DEAEARVIVLSAQGKHFCAGMDLANFQDNGEILGTDAKKVSQGRRGEAQFRLTRELQY
                     TISCLEKARIPVIAAIQGACIGSAVDMVTACDIRYATKDAFFCIQEINIGMAADVGTL
                     QRLPYLIPEGIVRELAYTGRKFSADEALRYGLINAVLDTHEAVIDHALDVAGEIANKA
                     PLAVTGAKEILNYNRDHSVEESLNYTALWNSAMNFSDDMMEAFKSKVEKKDPEFQGLV
                     KRKKHL"
     gene            complement(3455..3748)
                     /locus_tag="QGH35_01290"
     CDS             complement(3455..3748)
                     /locus_tag="QGH35_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015925.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HJL81201.1"
                     /translation="MSVIVILTLKAKPDSYDQLGSMLKEILSDTASFEGCKGLYACGD
                     QNSQTFLLYEEWESVEHQQKYLEWRTQRGDLDALGTMLREPPLFETRDFIFSS"
     gene            complement(3765..4652)
                     /locus_tag="QGH35_01295"
     CDS             complement(3765..4652)
                     /locus_tag="QGH35_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81202.1"
                     /translation="MLKSIMMVTMLVTNLNTIEEAYDTYLGYQVVEEGLIDRSLGSTW
                     GARGMNKHPYIIMQPSSGEEVYLRFIEDKSMTNYKPMGTHGWNSTEILVEDPDALAME
                     LSNSSFNIIGPPYDLYPTPNAPRAMQVLGPSNEILYLTRIIPEGSGFNLGSAQSFVDR
                     VFIMVVGGPSMKALQEFYKETLNMPVSEVSDWTIGVISRLNDLPEDTTYPLAIASFKK
                     NFLIELDEYPPIFLPRKRAVNHLPFSTSVVSFLVDSLDDFDIEWRRPPRLIQDFPYNG
                     RKVGVTIGPAGEWIELIEE"
     gene            4752..>5887
                     /locus_tag="QGH35_01300"
     CDS             4752..>5887
                     /locus_tag="QGH35_01300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81203.1"
                     /translation="MSSILPKDPLKYRWIILVMSFLLITITNGLTLGGLYVFEEELIN
                     SLSEITGEGVLRADLKLRDAILLWSTAFFGFFAGVIADKIGVKKLMISGLLLLSLCFW
                     QYSQAISLNQMYIIHIFMGLVLCIAGMLVNVILISRWFNNSRGLAIGILLAGTSTGNG
                     LFPQINTYLLSLGDWREVMIWLSYIPLCLIPLLLTFVKDSPEELQATTEEITDQESKE
                     SHTGKSFTLLQALKAKNFWLLSLMAFCTFYSILAMTGHVFLMLREENYAPQVAATGVS
                     MIFLGGFIGKVISGQLAETYGRKKILLSGVGIMLMGSIFIVLALLYSNPLFVWIGLGF
                     YGFGWGGLYTLIQLLSADLFGIASLGKIMGVINILDTFGGGLGP"
BASE COUNT         1774 a         1128 c         1093 g         1892 t
ORIGIN      
        1 tcaatggtcc agttacagcc atgatgtctg tcatctgtcc cacatcaagt aatgctgcta
       61 cacgtggcat caaatctcta ccgaaagtcg tcgaaggaca caacacacaa tcatattcag
      121 ttgcaaggtg agcaatgact ggggacataa cggctgcgac ctgatgatca aaacgttgat
      181 cttctagata cagaacagaa ttgacattct gaaacagagc agcttgggtt gctaattctg
      241 aattgtttcc caacactaac agatctgttt gttgtgaaga taaatcagac acacaggaca
      301 atgttttaga agtacttaca ttgatcacac catcgttgtg ttcagctatg actaagacat
      361 tcatattgat ctctgcatta attaatcaaa aaaacctttg gttttaagtg catcaaacgc
      421 ctcatcagca ttagttagca taattccact ctgcctgggt ggtggtgctg aaactttgat
      481 gactttaaag aatgcatctg tagaaacatt gaattctgat gaattcataa catccaatgg
      541 tttcttttta gctttcataa tatcaggcaa cttcacatac cttggttcgt ttaacctaag
      601 atcagttgtc actattgcag ggaggtctat ttctaaactt tcaaggcctt gatccacctc
      661 acgggtgact gtgaggacat tgccctcaat atcaagcttg gatatgaaag tagcttgggc
      721 ataagaacaa agttcagcaa gcatttgtcc tgtttggcta cagtcatcat caatagcttg
      781 tttaccaagc aaaataagtt ctggcttttc ttgctgaaca atttcataca ataacttggc
      841 aacgattaga ggttctgctt gttcttgatg ttcgactaat atggcacgat ctgctcccat
      901 agcaagcccg gtcctaagct gctgctgaca atcttctgat ccaatggaaa caacgataat
      961 ctcattggcc tttccagatt ctttaattct taaagcctcc tcaagagcga tttcatcaaa
     1021 agggttaata ctcatcttaa ccccttctgt gacgacgccg gactcatcag agttaatcct
     1081 gattttaata ttgtaatcaa taactctttt gatgcagaca agaatcttat tcataggggt
     1141 tcattggatc taattttgtg ttttagttgt cgagggatat ataatttaat gtgctattct
     1201 gacctaaata aaaaataata tacagaatat tttaaaaaaa tatctatgat ttctataaat
     1261 agaaaattct atttggttat atttttacaa tgcctattag tactcagtgg atgtacgcaa
     1321 gaagagcaat taaaaaatga acgatccact agcactggat acaatgtaag actagggtcg
     1381 agttgtttta acaaagacac cattcaagat tacagagtac ttgaccggtc caatttggtt
     1441 atctatggaa gacctaaatc aagatcatat cacttacaaa ttaccccacc agcatctatt
     1501 agaagctcag attccatcta ttttaattcg agaactggca gaatttgtgg ttttgctggt
     1561 gatgagttaa ttattcctga tagtattttt tcagaacgac attctattat tgccgttaat
     1621 gagttggatg acattgcaca ttacaatctt ttgctgcgtt ttggtatagg tgatccctta
     1681 gaggtggttg aacctgataa agagacatca ccagaaatta caagagaatt aagtgaaacc
     1741 gacaaccaag aaaatgacaa caatgaataa agaaagtatc ttgcagaaag atcaccagac
     1801 tgttgctgga aaaattattt acctcagtga aaaagaagac cgtcaaggcc aagagagagg
     1861 tagagaatat ttcataatca acaaacatgc taacggtcat agaaaaattg ttgctcactg
     1921 tgagattgat gatcgaccag ctgtaatgag ggatataacc tatagcctag atgaaaattg
     1981 gttgcctttg gattgttttg tcagaatttc tgtcaacgat cagttcatgg gctctggttg
     2041 gtttaatttt ggtgatgatt atgcagaatg tgaagcaaat acattgctcg atggcagaaa
     2101 tagtcaaaag atgcaaacca aaggtcgttt gaaaacattt cagaaccacg ccattgcttg
     2161 tgatgcctgg catttgcgat tgtatgacag gtctaaaaaa ggtccacaaa acataggtga
     2221 aatgctcctt tcttcacccg atcatagagg agcgaccggc ccaatgctat tctcaattac
     2281 ttcgacaata gatttccttg gagaagaatc agttaccatt aaagcaggga cattcgatgc
     2341 cttacatttt cagtttgttg gaacccctgg attacctcaa gaacatcccc cctacgatat
     2401 ttggtgtacc aatgatgaag actatctctt cttaaaaggc tcggttggtg ggtacatgca
     2461 aacatattac gagctagtgg aattgaaatc atcctgaact agttgttata aaaaccttgt
     2521 ccttatagat gcttttttct ttttaccaag ccttggaatt ctggatcttt tttctcgacc
     2581 ttggatttaa aagcctccat catgtcatct gagaaattca tagctgaatt ccaaagtgca
     2641 gtgtaattaa gggattcttc aacactatga tcacgattat aattcagtat ttctttagca
     2701 cctgttactg ccagtggggc tttattagca atttcaccag ctacatcaag tgcgtgatca
     2761 atgactgctt catgggtatc taaaacagca tttataagcc catacctcaa tgcttcatct
     2821 gcagagaatt tcctgcctgt ataggcgagc tccctgacaa tgccttctgg aattaaataa
     2881 ggaagtctct gtagggtgcc tacatcagca gccattccta tattaatttc ttggatacaa
     2941 aaaaaggcat ctttggtggc atacctaatg tcacaggcag taaccatatc aactgcacta
     3001 cctatgcaag caccttgaat ggccgcaatc acaggtattc tagctttttc taaacagcta
     3061 atggtgtatt gcaactcacg cgtcaatcta aattgagctt cacctcttct accttgacta
     3121 acttttttag catcagtgcc aagtatttca ccattatctt gaaaattagc aagatccatt
     3181 ccggcacaaa aatgcttacc ttgagctgaa agtacaatga ctctcgcttc agcttcatct
     3241 gatattttat ccactaattc tggcaattca gaccaaaatg ccttggtcat ggtattgaac
     3301 ttatctcctc tgctcataga gatatgcgct atgtgatctc ttaattcata ttcaaaacaa
     3361 ctgtaagtca taaaattctc ctcaattgcg gttagaacga tcagcttcta agtaacaatt
     3421 attctattaa ttctcattta tgcggtcatt atccttagct agaaaatata aaatctctgg
     3481 tttcaaaaag tggtggttca cgtagcattg tacccaatgc atcaagatcc cccctttgtg
     3541 ttctccactc taaatatttt tgctgatgct caactgattc ccattcctca tacagtaaaa
     3601 aagtttgact gttctgatca ccacatgcat agagtccttt acacccctca aatgaggctg
     3661 tatctgacaa aatctccttg agcatagagc caagttgatc gtaactatct ggttttgctt
     3721 ttaatgttaa tataacgatt actgacattt aattttcctc caaactattc ttcaattaac
     3781 tcgatccatt cacctgcagg tccaatggtt accccaacct tccttccatt gtaagggaaa
     3841 tcttggatca atctaggtgg tcgacgccac tcgatatcaa aatcatccaa agaatcaact
     3901 aaaaaagaaa caactgatgt tgaaaatgga agatgattga ctgcacgttt tctgggtaag
     3961 aaaataggag ggtattcgtc caactctatt agaaaattct ttttaaaact ggctatggca
     4021 aggggatagg ttgtatcttc tggaagatca ttgagtcttg atatgactcc aattgtccaa
     4081 tcggaaactt cgctaactgg catatttagc gtctctttat aaaattcttg tagtgctttc
     4141 atggatggac cacctaccac catgataaag accctatcta caaacgattg tgcactgcct
     4201 aaattaaatc ctgaaccctc aggaataatc ctggtcaagt atagaatttc attagatggt
     4261 ccaagtactt gcattgctct aggggcattt ggagttgggt acaaatcata gggtggacca
     4321 ataatattga agcttgagtt ggataattcc attgctagcg catcagggtc ttctactaga
     4381 atttctgtag aattccagcc atgagtaccc ataggtttat aatttgtcat cgatttatct
     4441 tcaataaacc gaagataaac ttcttcacca ctactgggtt gcataatgat gtatgggtgc
     4501 ttattcatgc ctcttgcacc ccatgttgat cccaaagaac gatcgatcag tccttcctcg
     4561 accacttggt aacccaggta agtatcataa gcttcctcga tcgtatttaa attggttacc
     4621 agcatggtaa ccatcatgat tgatttcaac attaacggat cctaattatt acaaacactg
     4681 attataaaat acttccttgt aataatagaa taatgtatgt ttcattaaag ttaaaaagga
     4741 tttaataatt tatgagcagt atcttaccta aagatccact taaataccgt tggataattt
     4801 tagtgatgtc ttttttactg atcactatca ccaatggact cacacttggt ggactatacg
     4861 tttttgaaga agaacttatt aattctctta gcgagatcac aggggagggt gttctcagag
     4921 cagatcttaa attaagagat gcgatcctcc tttggtcaac cgcttttttt ggtttttttg
     4981 caggtgtgat tgcagataaa atcggcgtta agaaattaat gatctctgga ctccttttac
     5041 tttcattgtg tttctggcaa tacagtcaag caatctcatt aaatcaaatg tacatcattc
     5101 atatttttat gggcctagtg ctttgtattg caggaatgtt ggtcaatgtt atcttgatct
     5161 cacgatggtt caataacagt agaggacttg ccattggcat tctactagca ggaactagta
     5221 caggaaatgg tttgtttcct caaatcaata cctatcttct atctttaggg gactggagag
     5281 aagtcatgat ctggttgagt tatattccat tatgtttgat tcctttgtta ctaacttttg
     5341 taaaagatag tcccgaagaa ttacaagcca ccactgagga aataactgat caagaaagta
     5401 aagaaagcca tacaggaaag agttttacac tattacaagc attaaaggct aagaatttct
     5461 ggttattatc attgatggct ttctgtactt tttactcaat tctagctatg actggccatg
     5521 ttttcttgat gctaagagaa gaaaattatg caccacaagt tgcagctaca ggcgtttcaa
     5581 tgatttttct aggtggtttt attgggaaag tgatcagtgg tcaattagct gaaacctatg
     5641 gtagaaaaaa gattttgcta tcgggtgtag gcataatgct gatgggttct attttcattg
     5701 tcttggctct gctttatagt aatcctttat ttgtctggat tgggcttggg ttttatggtt
     5761 ttggttgggg tggtttatat acgttgattc aattactcag tgcagattta tttggaattg
     5821 catctttagg taagattatg ggtgttataa atatattgga tacctttggt ggtggattag
     5881 gaccatt
//
LOCUS       DASCTH010000061         1743 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000084621, whole genome shotgun sequence.
ACCESSION   DASCTH010000061 DASCTH010000000
VERSION     DASCTH010000061.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000084621"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="QGH35_01305"
     CDS             complement(<1..1449)
                     /locus_tag="QGH35_01305"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HJL81204.1"
                     /translation="MNKSRIPNFYRLSIKDRVEAVFERGLISEEDYLALKNQQQQLDL
                     NSADKMIENVIGVMGLPIGLGLNFLINEKEYVIPLAVEEPSIVAALSSAAKIARKHGG
                     FKAESTDPILIGQIQVVGIKDLAKARSEILSRKKEVLNLANSFHPRMLARGGGAKDLT
                     VKTYPLDSFDQEMLIVDLFVDTRDAMGANLVNGMCEGVASLIESMTEGEVFLRILSNL
                     SDKSLATASVTIPTSALKIKGYDGEKVRDGIIIASDFAHVDPYRASTHNKGIMNGIDA
                     VALATGNDWRAIEAGAHAYAARHGRYSALSKWSIDDEGNLVGKIELPIKVGTVGGPME
                     SNPAVALNLRIINVESAMELARVMAAVGLAQNFSALKALVTEGIQTGHMTLHARSVVK
                     AAGTPIKFFDQVLEKLLIDGEIKVWKAREILEGLQKTMGTKSTKNLGRARKVSQSIEG
                     IGFGKVILLGEHSVVYGRHAIAVPVPLSVRVKV"
BASE COUNT          516 a          383 c          309 g          535 t
ORIGIN      
        1 aactttaacc ctaaccgata gaggtacagg gacagcaatt gcgtggcgtc catagacaac
       61 agagtgttca cccagaagga taactttccc aaaaccaata ccttctatgg attgggatac
      121 ctttcttgcc ctaccaagat tcttagtaga ttttgtgccc attgtttttt gtaaaccttc
      181 aagaatctcc cttgctttcc agactttaat ctcaccatct attaataatt tctccagtac
      241 ttgatcaaaa aactttattg gagtgcctgc tgctttgact actgatcttg catgaagtgt
      301 catgtggcca gtctgaatac cttcggtgac aagggccttt aacgctgaga aattttgagc
      361 taatcccaca gcagccatga ctctagccaa ttccattgcc gattcaacat tgattattct
      421 taagtttaga gcgacagcag gattcgattc catcggtcct cctacggttc caactttgat
      481 cggtagttct atttttccta caagattgcc ttcatcgtct attgaccatt ttgataatgc
      541 agagtagcga ccgtgtctgg cagcataggc atgtgctcct gcttcaatag ctctccagtc
      601 atttccagtg gctagtgcaa ccgcatcaat accattcata atacccttat tgtgagtaga
      661 ggctctgtaa ggatccacat gagcaaaatc agaagcaatg ataattccat ctctaacttt
      721 ttcgccatca tagcccttga ttttaagagc tgatgtgggg attgtgaccg aagcagtggc
      781 cagggattta tctgaaagat tagataggat tctcaagaaa acttccccct cagtcatcga
      841 ttctattagc gaagccacac cttcacacat tccattgacc aaattggcac ccattgcatc
      901 tcttgtatcg acaaaaagat caacgattaa catttcttga tcaaatgaat ctagtggata
      961 ggttttaaca gttaaatcct tcgcaccacc accacgggca agcattcttg gatgaaaact
     1021 attggcaaga tttagaactt ccttttttct acttagaatc tccgatcgag cttttgctag
     1081 atccttaatg ccaacaactt gaatttgtcc aatcagaatg ggatctgtag attctgcttt
     1141 gaaaccacca tgtttccttg caatttttgc agccgaactt aatgctgcaa caatcgacgg
     1201 ttcttcaact gctaacggaa tcacatattc tttttcattg attaaaaaat taagcccaag
     1261 accgatgggt aatcccataa caccaatgac attctctatc atcttgtcgg cactgtttag
     1321 atccagttgt tgctgttgat tttttagtgc cagataatct tcttctgaaa tcagacctct
     1381 ttcaaacacg gcttcaactc tgtctttaat ggacaagcga taaaaatttg gaattcttga
     1441 tttattcata tcctattaac ccaataagga catcataatg gcgataagag ttctttgatg
     1501 caactaattg ggtgaaaaaa aattaataca atgtaagaat aacaagttca catataatat
     1561 tataaggaat atgagcacag acaagccatg ggtgaaaaac tacccagaag ggacgcctga
     1621 tcacctagaa ttggatccga ataggacctt aataacactt ttagatgagg ctgaatccga
     1681 atttcaggaa cttactgctt tttcaaattt tggttcaact ctcagttata aagaggttga
     1741 gaa
//
LOCUS       DASCTH010000062         1722 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000087581, whole genome shotgun sequence.
ACCESSION   DASCTH010000062 DASCTH010000000
VERSION     DASCTH010000062.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000087581"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="QGH35_01310"
     CDS             complement(<1..1348)
                     /locus_tag="QGH35_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HJL81205.1"
                     /translation="MKNNKLKFILPIVMIAGAVSIGVTQSSLYRKIGQSQRLFNQVYN
                     QIFSTYVHELDPEAFTKASIQSITQNLDPYTVFMVEDEQHQVNVLSKGKYGGVGIQLG
                     YRNKTMSVVAPMDGGPAKKAGIISGDIILKVDGEDVKIMTFNEAASKIRGKQGTKVKL
                     TVKRYSEDEPIIFNLVRSVIKVKEVTYSGMLSPTTGYVRLNRFSRNTPNEMQQAFQQL
                     LNQNSKEIIIDLRDNPGGLLSSAVAILDMIIAKDSPLVSTKGRTKDSNRSFYSRRKPI
                     IPEDVKLAILINQGSASASEIVAGAVQDLDRGIVLGQKSYGKGLVQSQYEIDDKRSIK
                     VTTARYYIPSGRFIQKRDYIDDKYIMNKTEEDSIFTTSSGRKVLGNGGITPDSTITPE
                     LMETLSSQFWRRGYFYAFAQQNKHRYETFSDVLSDSEILIKFSEFINDQDDDIQLPG"
     gene            complement(1345..>1722)
                     /gene="tmk"
                     /locus_tag="QGH35_01315"
     CDS             complement(1345..>1722)
                     /gene="tmk"
                     /locus_tag="QGH35_01315"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HJL81206.1"
                     /translation="IQPNLSAGINVIADRYTDSTLAYQGGGRNIELEWLIQLNRFATY
                     NLEPNVTFFVDILPEEASRRKSQEKDRIERAGIDLQAKVRNAYLELVDRFQDRFVLIN
                     GHDTIENIHGSILLEIQRRHLFE"
BASE COUNT          491 a          352 c          291 g          588 t
ORIGIN      
        1 ctccaggaag ttgaatatca tcatcttgat cattgataaa ttcactaaat ttaatcagta
       61 tttctgagtc actaagtacg tcagaaaatg tctcataacg atgtttgttt tgttgagcaa
      121 atgcataaaa gtatccacgt ctccaaaatt gactggaaag cgtttccatt agttcaggag
      181 tgatggttga atcaggtgta ataccaccat tccctaatac ttttcgacca ctacttgttg
      241 taaagattga atcttcttcc gttttgttca ttatatattt atcatcgata tagtcacgtt
      301 tttgaatgaa acgcccgctg ggtatataat atcgtgctgt ggtgacttta atacttcgtt
      361 tatcatcgat ttcatattgt gattgaacca accctttacc atatgacttt tgtcccaata
      421 caatgccccg gtctaaatct tgaacggcac cagccacaat ttcacttgca gatgcactcc
      481 cttgattgat cagaatcgcg agttttacat cttctggaat aataggcttc cttctagaat
      541 aaaaagatcg atttgaatct ttggttctgc ctttagttga taccaacgga ctgtcttttg
      601 caataatcat gtccaagatt gcaactgcag aggaaagtaa tccaccgggg ttatctctta
      661 aatcaataat gatttctttt gaattttgat tcaagagttg ctgaaaagct tgttgcattt
      721 cattcggagt attccgtgag aatctattca gccgaacata gccggtcgta ggagataaca
      781 ttccagaata tgtaacctct ttaaccttaa taacggaccg aactaaatta aaaataatag
      841 gctcatcttc gctgtaacgt tttaccgtaa gcttaacttt tgtgccttgt ttacctctaa
      901 tctttgatgc cgcttcgttg aatgtcatta tttttacatc ttcaccatcc acttttaata
      961 taatatcgcc acttataata cctgcttttt ttgcaggacc accatccata ggcgcaacca
     1021 cggacatggt tttattccga taccctaatt ggatacccac accgccgtat ttccctttac
     1081 ttaagacatt aacttggtgc tgttcgtctt ctaccataaa aacggtatat ggatctaaat
     1141 tttgggttat gctctggata ctcgcttttg taaatgcttc cggatccaat tcgtgaacat
     1201 atgtggaaaa tatttgatta tatacctgat taaagagacg ttgagattgg ccaatttttc
     1261 gatataaact ggattgggtt actccgatag aaacggcacc agcaatcatt acaattggaa
     1321 gtataaattt taatttattg tttttcattc gaacaaatgt ctcctttgta tttctaatag
     1381 aatagacccg tggatgtttt cgattgtgtc atgtccgtta attaaaacga acctatcttg
     1441 gaatcgatcg accaattcaa ggtaggcatt ccgaaccttc gcctgtaaat caattccagc
     1501 tctttcaata cgatcttttt cttgactttt tctacgagat gcttcttccg gcaaaatatc
     1561 tacgaaaaac gtcacattag gttccaagtt atatgtagca aatcgattga gctgaataag
     1621 ccattctaac tcaatgttcc gaccgccacc ttggtaagca agggtcgagt ctgtatatct
     1681 atcagctatc acgttaatac ccgcacttaa attgggttga at
//
LOCUS       DASCTH010000063         4949 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000090893, whole genome shotgun sequence.
ACCESSION   DASCTH010000063 DASCTH010000000
VERSION     DASCTH010000063.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000090893"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="QGH35_01320"
     CDS             <1..252
                     /locus_tag="QGH35_01320"
                     /inference="COORDINATES: protein motif:HMM:NF016121.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HJL81207.1"
                     /translation="TFLVIYDPDGKILDTGVVKYREPYGGEIASPSWTAQFKGRNAKS
                     NYAVGGEIDGISGATISVYSMATGVMKLSLLFPLIKSQL"
     gene            249..743
                     /locus_tag="QGH35_01325"
     CDS             249..743
                     /locus_tag="QGH35_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81208.1"
                     /translation="MTELKSIRYLSSELKILVAVFIIVLTVGVTIGLIYVSYNTDYTL
                     SGTQTYYGGDAITSDFEIPEQYPKSLEGLLLTTHTHIIAFAIIFLIMGGLLFFTNIIH
                     KKWKLFFMIEPLISTLVTFGTFFAIRYIHHDFVYLTMVSGVLMYLSYYIMAFIIFRES
                     ISSN"
     gene            824..1375
                     /locus_tag="QGH35_01330"
     CDS             824..1375
                     /locus_tag="QGH35_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014016.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HJL81209.1"
                     /translation="MNAKIEKEIQLSSEDVIVLAMHGVPAKDTPVEKVGEYYKLHGMM
                     ETAPSHVVESIRYHFEELDDLVRNWPRTPANDLYYYGTKKVAAAISERTDTKVVMAFN
                     EFCAPDLSTAFADVVNEGAKRVLVVTAMLTPGGNHAEIDIPKAIKSAEKAHPDVEFIY
                     TWPFQIDAIADVLVDQIGNYVSE"
     gene            1450..3594
                     /locus_tag="QGH35_01335"
     CDS             1450..3594
                     /locus_tag="QGH35_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81210.1"
                     /translation="MGNILLKLFTYFVFLCFSCSIIVANEVQTISGNIRDGKTGLPLP
                     NVNIIINNEERGTTTDENGYFFIPGLPIGKHTVEFSHIGYTVHYEQVQIPFMGKLNIK
                     LFPALIQMDAMVVTGTRTERYLKDTPVTTQVIKGEKLTNSGAVDLSQIIQESTGINVV
                     ANQFGTGVELNGYGSNHILLLVDGVKVLGRVNGQLDISQIPINQIDRIEIVKGATSAL
                     YGSEAMGGVINVFTRTPINPISLTSELTMGSFGQQAQSLSFSKSNKNSHWTINETVRR
                     FGGYDLDDETIWEEGSQYDKRNIGLKYSRSVSDRMNVLFDTKYFSETQTLISNHVFKD
                     LSKNNRISVRGNIQYNLKNWVIKSSLEQSKYNHGFDRIVIKSGNLIKGSLTRDNLFSS
                     NFQLMRKGEKHNLLGGLGLDQEGIISDRVRRGDQNSDLMNVFIQDEIDISNKWVIIGG
                     LRMDQHSIYGKHISPKISIMFKPEMISRIRFAYGEGFRAPSFKELFLDYSNIAVGYHV
                     IGNSILNPEVSKNINLDIERWNTGKYHGRINFFWNEISGLIDYRHIGEVEGQTTFQSV
                     NLSSVRTRGFEVDLTYFITDQFEIWAGYSLLDTWDKDNERVLPLKARYKSQLGFRYSY
                     SNGLKLNLRVQTTGTRTSWEENNEGKSIKQIIDPYMIVNTHMSFPLPWGLSGFIGGKN
                     LTNYIDKVWGPMPGREWYGGIRFDIRKKQKRN"
     gene            3597..4148
                     /locus_tag="QGH35_01340"
     CDS             3597..4148
                     /locus_tag="QGH35_01340"
                     /inference="COORDINATES: protein motif:HMM:NF025430.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HJL81211.1"
                     /translation="MKNTLLWLSIIGMTIIGCEDKKAQENIVIDESIKEFISVNMKAN
                     GPEYFIFSENNGVTTEPSKWDLSFAVVDYQPSPMAPVIKDPVIIIGNGKMAATIEAKS
                     LTDVTTLPAASLFKSDKDGFYTTQGWYDYNPTNHMITPKEFVYVVKIDETKNALIEII
                     DYYNGNGESGYITIHWKFLANNI"
     gene            complement(4301..>4949)
                     /locus_tag="QGH35_01345"
     CDS             complement(4301..>4949)
                     /locus_tag="QGH35_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81212.1"
                     /translation="SAENYHNEDLRLESMIPYVQGKLPIYVHANEVRQIEAAVHWSKR
                     QDVNIIIVGGKDAWRTTNLLRKNKVPVIYESVTALPNRRYEDYDQAYKGPALLHEAGV
                     QFCIASSGSAGGAYRVRNLPNQAAMAAAYGLPKNEALRSITLSAAEILGIDKQVGSLE
                     SGKDATLFISNGDALEIRTNVLQAYIQGRKVDMGDRHKMLFHKYQEKYRQLDILK"
BASE COUNT         1571 a          865 c          998 g         1515 t
ORIGIN      
        1 acatttctgg tgatttacga tcctgatgga aagattcttg atacaggtgt ggtcaaatac
       61 agggaacctt acggcggcga aatagccagc ccaagttgga cggcacaatt caagggccga
      121 aacgcgaaat cgaactatgc agttgggggc gaaattgacg gaatttctgg ggccacgatt
      181 tctgtatata gtatggcgac cggtgtcatg aaactgagtc tcctttttcc cttaatcaaa
      241 tcgcagttat gactgaatta aaatccattc gatatctttc aagtgagtta aaaatactcg
      301 ttgcagtatt tattattgtc ctgactgttg gtgtaactat tggattgatc tatgtaagtt
      361 ataatacaga ttacacttta tcgggcaccc agacctatta tggtggagat gcgatcacat
      421 ccgatttcga aattcctgaa caatatccaa aatctcttga aggattattg ttaacaaccc
      481 atacccatat aattgcattt gccattatat ttttaataat gggcggattg ctatttttta
      541 caaatataat ccataaaaaa tggaaattat tttttatgat tgaaccatta atttcaacct
      601 tggtgacatt cggaacattt tttgccattc ggtatattca ccatgatttt gtttacctca
      661 caatggtatc tggagtttta atgtatttat catactatat aatggcattt atcatttttc
      721 gcgaatcaat ttcatcaaat taatcaaata ataaaggaat taaatcatgt ttaaacgttt
      781 ttcaatcata atcttatcct gtattctttt aggtggctgc agtatgaatg ccaaaataga
      841 aaaagaaatt caattaagtt cagaagatgt tattgtgctg gccatgcacg gtgttccggc
      901 caaggatacg ccggtagaga aggtgggtga atactacaaa cttcatggaa tgatggaaac
      961 agcgccgtct cacgtagttg aaagtatccg atatcatttt gaagaattgg atgacttggt
     1021 gcgaaactgg ccccgaacac ctgcaaatga tctctattat tatggtacta aaaaagtcgc
     1081 cgctgcaatt tcagaaagga cggatacaaa agtggtgatg gccttcaatg aattctgcgc
     1141 accggatctt tcgacggctt ttgcagatgt ggtgaatgaa ggagctaaaa gagtacttgt
     1201 tgtcactgcc atgcttacac ccgggggcaa ccatgctgaa atcgatattc ccaaggctat
     1261 caaatccgct gaaaaggcac atccagatgt tgaatttatt tatacatggc cttttcaaat
     1321 tgatgccatt gccgatgtcc tcgtagacca gatcggtaat tatgtatccg aataattttt
     1381 ccttttactt aaatgaattt ctaagaacac gatcggagat ctaggccctt gaaaaaaacc
     1441 aatagttata tgggtaatat tttgttgaaa ctctttacat attttgtttt tttatgtttt
     1501 tcctgctcca tcattgtagc caatgaggtc caaacgatat caggtaatat ccgtgatggg
     1561 aagaccggtt tacctctacc aaatgtgaat atcattatta ataatgagga gcgcggtaca
     1621 accacggatg aaaatggata ttttttcata cccggattac caataggtaa gcatactgtt
     1681 gaattttcac atattggtta tacggtccat tatgaacaag ttcagattcc atttatggga
     1741 aaactaaata taaaattgtt ccctgcccta atccaaatgg atgccatggt ggtcaccggt
     1801 acgcgaacag aacggtactt aaaggatacg cctgtaacaa cccaagtgat taaaggagag
     1861 aaactcacaa attcaggtgc ggttgattta tcccaaatta ttcaagaatc tacaggcatc
     1921 aatgtggtcg caaaccaatt tggaactggc gtggaattaa atggttatgg ttccaatcac
     1981 attttactct tagtagatgg tgtgaaagtt ttgggtcgag taaacggtca attggatata
     2041 tctcaaattc ctatcaatca aattgatcga attgagatcg ttaagggcgc gacttcagct
     2101 ttgtatggca gtgaagcaat gggtggagtg attaatgttt tcaccaggac accaataaat
     2161 cctatatccc tgacttccga gttgacgatg ggttcattcg gtcaacaagc acaatccctg
     2221 tcatttagta aatccaataa aaacagccat tggacgataa atgaaaccgt taggcgtttt
     2281 ggagggtatg acttagatga tgaaaccatt tgggaagagg gaagtcaata tgataagcga
     2341 aatattgggt taaaatatag tcgatctgtc agtgatcgga tgaatgtgtt atttgataca
     2401 aaatattttt cggaaactca aacactgatc agtaaccatg tctttaagga tttatcaaaa
     2461 aataaccgta tatctgttcg tggaaatatt caatataatc tgaaaaactg ggtgattaaa
     2521 tctagccttg aacaatcgaa gtataatcat ggttttgatc gaattgtaat taaatcaggc
     2581 aatctaataa agggatcttt aactagggat aatttatttt cttcaaattt tcaattgatg
     2641 cgaaaagggg agaaacataa cctcttaggg gggctgggtt tagatcagga aggaattata
     2701 tctgatcggg tacggagagg tgatcagaat tctgatttga tgaatgtatt tattcaagat
     2761 gaaatagata tttccaataa gtgggtcatt atcggtggat tacgaatgga tcaacattcc
     2821 atttatggaa aacatatctc tccaaaaata tctattatgt tcaaaccaga aatgatctct
     2881 cgtattcgat ttgcatatgg cgaaggtttc cgtgctccat cttttaagga attatttttg
     2941 gattacagca atattgcagt gggataccat gtcattggca attcgatttt aaacccggaa
     3001 gtatcaaaaa atatcaatct tgatattgaa agatggaaca cggggaaata tcatggccga
     3061 attaattttt tctggaatga aattagtgga ctaattgatt atagacacat aggagaggta
     3121 gaaggacaaa ctacattcca atctgttaat ctatcttctg ttcgtactcg aggtttcgag
     3181 gtggatttga cctattttat taccgatcag tttgaaatat gggcaggata tagcctatta
     3241 gacacatggg ataaggataa cgaaagagta ttacctctaa aagcacgcta taaatcgcaa
     3301 ttgggttttc gttattcata ttcaaatggt ttaaaactaa atcttcgagt acaaaccaca
     3361 ggaacaagaa ccagctggga agagaataat gaagggaaat caataaaaca aatcatcgat
     3421 ccctatatga tagtaaatac gcatatgtct tttccactgc cttggggatt gagcggtttt
     3481 attggtggga aaaatttaac taattatatc gataaagttt ggggccccat gcctgggaga
     3541 gaatggtatg gaggcatacg tttcgatatt agaaaaaaac aaaaaaggaa ctaatcatga
     3601 aaaatacatt attatggctt tcgattattg gtatgaccat aatcggatgt gaagataaga
     3661 aagcacaaga aaacattgta attgatgaat caataaagga gttcatttcc gtgaatatga
     3721 aagcgaacgg acccgaatac tttatttttt ctgagaataa tggtgttaca acagaacctt
     3781 ccaagtggga tctatccttt gcagtagtgg attaccagcc ttcgcctatg gcaccggtga
     3841 taaaggatcc ggtaatcatc attgggaacg ggaaaatggc agctacgatt gaagccaaat
     3901 ccttaaccga tgtaacaacc cttcctgcgg catctttgtt taaatccgat aaagatggtt
     3961 tttacacaac ccagggatgg tatgattaca acccaacgaa tcatatgatt acaccaaagg
     4021 aattcgtgta tgtcgttaaa attgacgaaa ctaaaaacgc cctaattgaa attattgatt
     4081 attataatgg aaatggtgag tcaggatata taacgattca ctggaaattt ttggcgaata
     4141 atatatagat tgatattggt atgggagcaa aaaaacttct gatttgctaa acctcctcat
     4201 taacctcctt ctaggaatca ggattcgttt tcgtgccaaa tattaaaaaa agagggctga
     4261 ttaatcagcc ctctttttat acttattttt ataagattta ttattttaaa atgtccaatt
     4321 gccgatactt ttcctgatat ttatgaaaca gcattttatg ccgatctccc atatccactt
     4381 ttcggccttg gatataggct tggagcacat tggttcttat ctcaagtgca tcaccatttg
     4441 aaatgaataa cgtggcatct tttccagatt ctagggaacc cacttgtttg tctataccca
     4501 atatttctgc tgcagataag gtgattgatc ggagtgcctc atttttgggt aagccataag
     4561 ctgcagccat ggccgcttga tttggcaagt ttcgtacacg gtaagcaccg ccagcagaac
     4621 cggaagaggc aatacaaaac tgaacgcctg cctcatgaag taaggccggt cccttatagg
     4681 cttggtcata atcttcatat cgccgattcg gtagtgctgt tacgctttca taaatgactg
     4741 gaactttatt ttttcggagc agattagtgg tccgccatgc atctttgccg ccaacgataa
     4801 taatattaac atcctgtcgt ttggaccaat gaacagcagc ttcaatttgg cgtacctcgt
     4861 ttgcatggac ataaataggc agctttccct gaacataggg aatcatactt tcaaggcgca
     4921 aatcctcatt atgataattt tcagccgac
//
LOCUS       DASCTH010000064         1796 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000093202, whole genome shotgun sequence.
ACCESSION   DASCTH010000064 DASCTH010000000
VERSION     DASCTH010000064.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000093202"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(29..958)
                     /locus_tag="QGH35_01350"
     CDS             complement(29..958)
                     /locus_tag="QGH35_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81213.1"
                     /translation="MSAHKIVLIFSALLMVSGCAIQPMSSGSPSASGSSMPSGPQMPS
                     GSSGSPSPPQGSSSSASQSSSSGVSISSGGQSVSGSSMPSGQGTPSGSSGSPSTTSSS
                     GTSQGTASASDTGSSGTQEQQSGSNSQQGQSNTQTGVSSSGQTQADPSQTGSQESAMG
                     SMSGSNSAASVFDESLGVFDQEMASERIVIASSGEGSDGMSAQEAADASAVQSGLLEG
                     ELPMENASGESSIGAAGDQDQDEASSQQQGDSNESSHQSEQMKERIPDDIPMDGTGED
                     VIARQIREAAIQEGDSEIREALWDEYRKHMGIR"
     gene            1111..1578
                     /locus_tag="QGH35_01355"
     CDS             1111..1578
                     /locus_tag="QGH35_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HJL81214.1"
                     /translation="MSKENVLNELKCQVGTLIHQSNWIAISQIMIDAFANATDDHQWI
                     HIDQEKAAKESPFKATIAHGYFTLSLYPKLRGLVDPKDPPFPGTKNIINYGINKLRFP
                     GPVISGSKIRSNCTLLSVEEVKGTIELIEEYSVEVEHQDRPACVAECIIRLYF"
     gene            1585..>1796
                     /locus_tag="QGH35_01360"
     CDS             1585..>1796
                     /locus_tag="QGH35_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJL81215.1"
                     /translation="MSDILQERQSSYLKLTINRPEASNALSLDLFDKLSEILSENRND
                     PDLKSVVVTGQGNKSFAAGGDIKKLN"
BASE COUNT          542 a          385 c          361 g          508 t
ORIGIN      
        1 tcagccggct aaacataatt tcttaatttt atctaattcc catatgtttt ctatactcat
       61 cccacaatgc ttctcgaatc tctgagtcac cttcttggat agctgcctct ctgatttgtc
      121 gagcaatcac atcctctcca gtgccatcca tggggatatc atcggggatt ctttccttca
      181 tttgttccga ttgatggctg gactcatttg aatcaccttg ttgttgggaa gaagcttcat
      241 cttgatcctg atcaccggct gcacctatgg aagattctcc ggaagcattt tccatgggta
      301 gttctccctc caaaaggcct gactgaaccg cagatgcatc tgcagcttcc tgagcactca
      361 ttccatctga tccttcaccg gatgaagcaa tcactatcct ttcacttgcc atttcttgat
      421 caaaaactcc caatgattcg tcaaatacac tagcagcaga attactacca gacattgaac
      481 ccatagctga ttcctgcgat ccagtttgtg aagggtctgc ttgtgtttga ccagaagatg
      541 aaactccggt ttgtgtattg gattgacctt gctggctgtt tgatccagat tgctgttctt
      601 gtgtaccact agacccggta tcactagccg aagcagtgcc ttgagatgta ccagaagaac
      661 ttgttgtgct gggagaacca ctagacccag atggtgtacc ttgtccagaa ggcattgatg
      721 agccggacac agactgacct ccactgctta ttgaaacgcc agaagaagag ctttgagaag
      781 cactagaaga agagccttga ggagggctag gggaaccact agatccagat ggcatttgtg
      841 gacccgaggg cattgatgag ccagatgctg agggactgcc gctactcatt ggctgaatag
      901 cgcaaccact aaccattagc agagctgaaa agattaaaac tattttatgt gctgacatat
      961 tcataatccc aaagccagct tagcaggact gatttaatta tagcatcaaa atatatagaa
     1021 aataatgcac gtaaaactca atcactactc gtcacatagt cttattattt gataggattt
     1081 ctctctaata tcaatttatc gggagtgata atgagtaaag aaaatgtttt aaatgaatta
     1141 aagtgccagg ttggaacttt aattcatcaa agtaattgga tcgcaatcag tcaaataatg
     1201 atcgatgcat ttgccaatgc aactgatgac catcagtgga ttcatataga ccaagaaaaa
     1261 gcagccaaag aatcaccgtt taaagcaact attgcacacg gttattttac gctatctctt
     1321 tatccaaaat taagaggact cgtcgacccg aaagatccac ccttccctgg tacaaaaaat
     1381 attattaact atgggatcaa taaattgaga ttcccagggc ctgtaatttc tggcagcaaa
     1441 atcaggtcaa attgcactct gctttctgta gaagaagtca aaggtactat tgaattaata
     1501 gaagagtatt cagttgaagt ggaacatcaa gacaggcctg cttgtgttgc ggaatgcatt
     1561 atccgattgt atttttaatt tactatgtca gacatactgc aagagcgaca gtcatcttac
     1621 ctaaaactca ctatcaatag accagaggca agcaatgcgc tcagccttga tttgtttgac
     1681 aaactcagtg aaattttatc tgagaacaga aatgatcccg atttaaaatc agtggttgtc
     1741 actggacagg gaaataaaag cttcgcagct ggtggagata tcaaaaaatt aaataa
//
LOCUS       DASCTH010000065         2848 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000093246, whole genome shotgun sequence.
ACCESSION   DASCTH010000065 DASCTH010000000
VERSION     DASCTH010000065.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000093246"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(128..1594)
                     /locus_tag="QGH35_01365"
     CDS             complement(128..1594)
                     /locus_tag="QGH35_01365"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJL81216.1"
                     /translation="MKQEHVNVVIVGAGISGIDAAYNLQTKCPNKSYAILEGRECLGG
                     TWDLFRYPGIRSDSDMCTMGFRFKPWEGEKIVADGSSILKYLRETVDEYGIDEHIHYS
                     QMVQTASWSSAESKWTLGVENQKTQELQTFTCDFLFLCGGYYSYEKGYNPHFEGRDDF
                     SGQIIHPQEWPEDLDYANKNVVVIGSGATAVTIVPSIADEAAHVTMLQRSPTYYLIAP
                     EEDAMGNFLRKYTNSKIAYFLVRWRNIFVSQWFFFQKCRKYPKKTKEFLINLVKESLD
                     QNYDVDTHFTPNYNPWEQRMCLVPDGDLFNAIKSGKASVVTDHIERFTTTGIKLKSGD
                     ELDTDLIVTATGLNLLVCNGIELFVDNEQIDVSTKMTYKGMMFNDIPNLVATFGYTNA
                     SWTLRADLTSEYVCRVLNFMDNKNFDYCCPRVDGIVEDDGNWLDFSSGYVVRSMHKFP
                     KQGSRDPWRNTQNYLKDIFAIRFGSLENKELEFTVAKN"
     gene            1765..>2848
                     /gene="dnaX"
                     /locus_tag="QGH35_01370"
     CDS             1765..>2848
                     /gene="dnaX"
                     /locus_tag="QGH35_01370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HJL81217.1"
                     /translation="MSYLVLARKYRPKLFSEVSGQDHVVKALQNSLTNNKVHHAFLFA
                     GTRGVGKTTIARLLAKALNCETGIVSEPCGQCAICIAIDEGNYLDLIEVDAASQSKVE
                     ETRELLENTQYTPTIGRFKIYLIDEVHQLSKHSFNALLKTLEEPPPHVKFLLATTESH
                     KLPITVLSRCMKFNLKKISSELIKSRMKEICDAEKDVDYEDNALTILSKTADGSMRDG
                     LSLLDQALAFEDYSLTANQVELMLGTIDINNIFGLLTSIIDQDPVTLNQGLHDLDDLY
                     PDYKSLLDNMASILQQIAFLQITGSKQLDSEQEVDKAVIDFATKIQPEVIQLLYQITI
                     TSKRDIDLAPSQREGFAMAILRMFAFT"
BASE COUNT          882 a          541 c          522 g          903 t
ORIGIN      
        1 tctgaggaac cctattcagt caccttgctg agtttccaac tgttgtctgg tttgtctcta
       61 cctgctttgt attatgctgg aaagaactat aaaagtgata gagagcaaat gctggcacaa
      121 gaaaacatta gttcttcgcc acagtaaatt ctaattcttt attttcaaga ctcccaaatc
      181 tgatagcaaa gatatctttt aaataatttt gagtatttct ccatgggtct ctggaaccct
      241 gtttaggaaa cttgtgcata gatctgacca cataaccaga tgaaaaatcc aaccaatttc
      301 catcgtcttc aacaatgcca tccactctag gacaacaata atcaaagttt ttattatcca
      361 tgaaatttaa tactctacag acatattctg aagtgagatc agccctcagt gtccaggatg
      421 cattagtata gccaaatgtc gcaactagat taggaatatc gttgaacatc atgcccttgt
      481 aggtcatttt tgtggataca tcaatttgtt cattatcaac gaataactct atcccattac
      541 aaactaataa atttaagcct gttgcggtta caatgagatc tgtatcgagt tcatctcctg
      601 atttaagttt gatccctgtt gttgtgaatc tttcaatatg atctgttaca actgacgctt
      661 ttcctgattt aatggcatta aataaatcac catctggtac caaacacatc cgttgttccc
      721 atgggttata gttaggagtg aaatgggtat caacatcata attttgatct aaggattctt
      781 tgactaagtt gataagaaat tctttggttt ttttaggata ttttctgcat ttttgaaaaa
      841 agaaccattg tgaaacaaaa atgtttctcc aacgaaccag aaaataagct attttagagt
      901 ttgtatattt tctcaaaaaa tttcccatag catcttcttc gggagcaata agataataag
      961 tgggtgatct ttggagcatc gtcacatggg ctgcttcatc tgctatggaa gggacaatag
     1021 ttacagcggt agcgccactc cctataacta ctacgttttt atttgcatag tcgaggtctt
     1081 ctggccattc ttggggatga atgatttggc cagaaaaatc atctcttccc tcgaagtggg
     1141 gattgtatcc tttttcatag ctgtaataac caccgcagag aaataagaaa tcacaggtga
     1201 atgtttgtag ttcttgtgtc ttttgatttt caacacccag tgtccatttg gattctgcag
     1261 atgaccaaga ggcggtttgg accatttgac tatagtgtat atgttcatcg atgccatatt
     1321 catcaaccgt ctcacgtagg tatttgagta tagaggagcc atccgcaaca attttttctc
     1381 cctcccaggg cttgaatcta aagcccatcg tacacatatc agagtcagat ctgataccag
     1441 ggtatcgaaa taagtcccat gttcccccaa ggcattccct gccttcaagg atggcataag
     1501 atttgtttgg gcactttgtc tgtaagttat aagcagcatc aattccagag ataccagctc
     1561 ctacgatgac gacattaaca tgttcttgtt tcatttgaat atcagctttg ctcctcaatc
     1621 tataggaaac agactacatt ctaataatta acttgtaaat aaagtaaaag gacctttatt
     1681 aaggactttt tttttataca atagaaatcg ttagtttttt caacaatctg tattatttta
     1741 tttatttttt tattaatttg tttaatgagt tatctagttt tagcaaggaa gtataggcct
     1801 aaattatttt ctgaagtctc aggacaagat catgttgtca aagctcttca aaattcgtta
     1861 accaataata aagtacatca tgctttttta tttgcaggca caaggggtgt tggcaaaacg
     1921 accattgcga gattactggc caaagcgttg aattgtgaga caggcattgt ttctgaacct
     1981 tgtgggcagt gtgcaatttg catagccatt gatgaaggca attatctcga cttaatcgaa
     2041 gttgatgccg catcacaatc aaaggttgaa gaaacacgtg aactccttga aaacacccaa
     2101 tatacgccga cgatcggcag atttaagatt tacctcattg atgaggtgca tcaattgtct
     2161 aaacattcat ttaatgcttt gttgaaaacc ttagaagaac cacctccgca tgtgaaattt
     2221 ctcctagcca ccactgagag tcataaactt cccattacgg tattatcaag gtgcatgaaa
     2281 tttaatctta aaaaaatctc atcagagtta atcaagagca gaatgaagga gatctgtgat
     2341 gcagaaaaag acgtagatta tgaagacaac gctctaacaa tattaagtaa aacagcagat
     2401 ggcagcatga gagatggatt gagtttgtta gaccaggcgt tggcttttga agattattca
     2461 ttgactgcta atcaagtaga actcatgtta ggcacgattg atatcaataa tatttttgga
     2521 ttattgacct ccatcatcga ccaagaccct gtgacattga atcagggttt gcatgattta
     2581 gacgatctct acccagatta taaatccttg ctcgataata tggcttcaat tttacagcaa
     2641 atagctttcc ttcaaattac tggtagcaaa caactcgata gtgaacaaga agtggataaa
     2701 gcggtgatag attttgctac aaaaattcag ccagaagtca tccagcttct ctatcagatt
     2761 accatcacat ccaaacggga catagattta gcaccaagcc agagagaggg gtttgcaatg
     2821 gcaatattaa gaatgttcgc attcactg
//
LOCUS       DASCTH010000066         1882 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000096737, whole genome shotgun sequence.
ACCESSION   DASCTH010000066 DASCTH010000000
VERSION     DASCTH010000066.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000096737"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="QGH35_01375"
     CDS             complement(<1..592)
                     /locus_tag="QGH35_01375"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HJL81218.1"
                     /translation="MTENNDKRAFKPKKAYKNLEFLNSKDARTIRMLAEYLYPKTQLE
                     KEGVQNTIVIFGSARAPSPEELETSTEERGRLAKYYNYTRELTAKLSKWAKALEENEQ
                     TYVVCSGGGPGIMMAANRGAHDENAKNIAFTISLPNENIPNPYATPDLNFEFHYFFTR
                     KFWFSYLAKALIVMPGGFGTLDELFEILTLIQTGKIN"
     gene            complement(772..1737)
                     /locus_tag="QGH35_01380"
     CDS             complement(772..1737)
                     /locus_tag="QGH35_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJL81219.1"
                     /translation="MSRLIKKIFTFLCALFGLCYIGLIIYAYMPNQEYPIESFATKDD
                     QFADINGQKIRYREFNEGTNDKPYLILIHGFGHSLDSWRRVRPELQKYYHVVALDLIG
                     FGLSSKPVDYDHSNENQAATIGLFADYLGIEKFIIGGHSLGGAIAVHIALNNKKTTGA
                     ILFNPGIINTGVPKIFKYINPIFPFSRVTAKQFSNREFRTKFLKRSFVNPSIVTDEVM
                     DEVMLAVQTEDYMDGTASLMKKFNDPHEFELLPFIEVPTLIVFGMQDRNKSLDEAKAL
                     NDNIRGSKLVLVQQAGHYVHEEQPEIVAKIIIENLSFLSARIMGK"
BASE COUNT          555 a          363 c          305 g          659 t
ORIGIN      
        1 tattaatttt tccggtttgg atcaaagtta atatctcaaa aagttcatct aaggttccaa
       61 aacctccagg cataacaatc aaagctttag ctaaatatga gaaccaaaac tttctggtaa
      121 aaaagtaatg aaattcaaaa tttaaatctg gagttgcata tgggttggga atgttttcgt
      181 ttggaagaga aatagtgaaa gctatatttt ttgcattctc atcgtgagct cctctattgg
      241 ctgccatcat gatgccgggt cctccacctg aacagacaac ataagtttgt tcattttctt
      301 ctaatgcttt tgcccatttt gaaagtttag ctgtcagttc tcttgtgtaa ttatagtatt
      361 tagctaatct ccccctttct tctgtacttg tttctaattc ttcaggagat ggtgcacgtg
      421 ctgaaccaaa tattactatt gtattctgaa ctccttcctt ttctaattgg gtttttggat
      481 ataagtattc agctagcatc cttatggttc tagcatcttt gctgtttaaa aactctaggt
      541 ttttatatgc cttctttggt ttaaaggctc ttttgtcatt attttcagtc atatttttga
      601 gaccaattgg ttacataatt ttattcaaca tcaggttcag gatccattga acttccatgg
      661 gctacctccc acaaaaaacc atgctaccct caatctgatg aaagttaatc tttgttccat
      721 gccttcacta ttcttcattc tcactcaata taagattatc ttttaggacg attattttcc
      781 cataattctc gcacttaaaa aactgagatt ctctatgata atttttgcta cgatttcagg
      841 ttgttcctca tgaacataat gacctgcctg ttgtaccaat accagcttac tacctctgat
      901 attatcgttt aatgctttag cttcatcgag gcttttgttt ctatcttgca taccaaagac
      961 aatcaatgta ggcacctcaa tgaatggcag taattcgaat tcatgtggat cattaaattt
     1021 tttcatcaag cttgcggttc catccatata gtcttctgtt tgaacagcaa gcataacttc
     1081 atccatgacc tcatcggtca caattgaagg gttaacaaag gatcttttta aaaattttgt
     1141 tctgaactct ctgtttgaga attgctttgc agttacacgg ctaaatggaa aaataggatt
     1201 aatatatttg aatatttttg gcacacctgt attaataata cctgggttaa ataaaatcgc
     1261 accggttgtt tttttattat taagagcaat gtgtacggct atcgcaccac cgagtgagtg
     1321 ccctccaatg ataaactttt ctatacccag gtaatcagcg aataagccaa ttgttgcagc
     1381 ttgattctca ttactgtgat cataatccac aggttttgaa gaaagaccaa aaccaattag
     1441 atcgagagca acaacatggt aatacttttg tagttctggc cgcactctcc tccaagaatc
     1501 taaagagtgg ccaaagccat ggatgagaat caagtagggt ttatcgtttg taccttcatt
     1561 aaattctctg taacgtatct tctgtccatt gatatcagca aattgatcat ctttagttgc
     1621 aaaagattca attgggtatt cttggttagg catgtatgca taaataatta atcctatata
     1681 gcacaatcca aacaatgcac acaaaaaagt aaaaattttt tttattagtc gagacattag
     1741 gatagtttat tgtcgcctaa caaatagagc tcgattatcc attagaaatg gatgtaaatt
     1801 ccaacttaga atgcagtatt ccgttgagtt cttagttctt aggttccaac caatcgccgg
     1861 caaatccaca gccaaattct ac
//
LOCUS       DASCTH010000067         3011 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000097853, whole genome shotgun sequence.
ACCESSION   DASCTH010000067 DASCTH010000000
VERSION     DASCTH010000067.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000097853"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            223..633
                     /locus_tag="QGH35_01385"
     CDS             223..633
                     /locus_tag="QGH35_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81220.1"
                     /translation="MSRKSLFCALVVIVFFSFEAQSAVTEVNGCNLKKGASIEDITAM
                     SVKMNELMDSDDYKEHRFGQLVMEPYVEQTEKSEFDFYYLNYWGNYEIFGNDMHEWFD
                     HEKGKEWSSELSKMVDCRTLNIFDTIVTRQYPGD"
     gene            640..1125
                     /locus_tag="QGH35_01390"
     CDS             640..1125
                     /locus_tag="QGH35_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019932.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HJL81221.1"
                     /translation="MKKIILLFGLLTISPLWADGHEKEVLDLVYKHWEARDNSDFATQ
                     ADLMSQSGTYNANSDGSFFRFSKQNSAADMEKNMGDQSSSLNVMYPETIALADNVVLA
                     RYYLEGVISDPGGTVSNYRTRVTHIWVKEKGGWKTKSWHFSPLHDGGRHLTSASDFEE
                     E"
     gene            1157..1840
                     /locus_tag="QGH35_01395"
     CDS             1157..1840
                     /locus_tag="QGH35_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81222.1"
                     /translation="MKKILFITSLACIFFANNIFAVEMDVYVYKVKPGSLEEALEIFQ
                     EGSSIASQTGMNVAIAQQSSGKGGELTYHWLEFYDSLEQRAEIRYADPKWDAYTEKFY
                     SSNAIDTVRTYSMTSLDEDIGDENVALARVYVWDAKPNMYDETITALFQAKEIFEKHG
                     FVVDLWQQGVGSKHYLEFVMLSSSEEEQAKSLRSVNADQDWIEIEPVWMKNEEVARFI
                     TSFELSKIN"
     gene            1976..2392
                     /locus_tag="QGH35_01400"
     CDS             1976..2392
                     /locus_tag="QGH35_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81223.1"
                     /translation="MKYFNLVLFTVILSFNLSAAHHDEDEQKGISGLISSEVFDLAGE
                     MDLYVERYQSCGDTNNEKHYHPVGTLVYMLDGKAASISSGEWKEYSEGSYWFEPSMWV
                     HGGSMTDEPKFGDDQCRQTLVIRASRKGEEPTVFVK"
     gene            2408..>3011
                     /locus_tag="QGH35_01405"
     CDS             2408..>3011
                     /locus_tag="QGH35_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81224.1"
                     /translation="MKVFKKYFTTIGLLIFSAQLIAGPGLAVTTINTKDAVTYTKWLT
                     ESTPKFMEVYGETVASTGICSPISGAEQEGDHYAWAIAPSMSALLQSEVRFWNDKSIA
                     KTINKISSKRNIVRKDIYEIIKPAKRAYKVGETTAQYNLLSRPNNVAEYVKTIEAMEA
                     AAAENGFEDIQMVVFEGFGAGDWARMVMASIQAPNVERLGA"
BASE COUNT         1020 a          432 c          651 g          908 t
ORIGIN      
        1 acttatttcc tctgaggagt atgcaagaga agctaaaatt tggattgatc aaggtgctta
       61 tgtggttggt ggttgctgta ccacaagacc agaacatatt aaaaaaattg cagatcaatt
      121 gaaataatag atcgaggcat gtgcaattta ttgacccaat ttggaatcat attgatatct
      181 gttatcattt agattcttaa taatttaata ggggaaatta tcatgtcaag aaaatctctt
      241 ttctgtgcat tagttgttat tgtcttcttt tcctttgaag cacaatctgc agttactgaa
      301 gtaaatggtt gtaatctgaa aaagggagca tcaatcgaag atatcactgc catgtctgtc
      361 aaaatgaatg agctaatgga tagcgatgat tataaggaac acagatttgg gcaattagta
      421 atggagccct atgttgaaca aacagaaaaa tcagaatttg acttttatta cctaaattat
      481 tggggtaatt acgaaatatt tgggaatgat atgcatgaat ggtttgatca tgaaaaaggt
      541 aaagaatggt catctgaatt atcaaaaatg gtcgattgta gaacactaaa tatttttgac
      601 acaatagtaa ctcgtcaata tccaggagat taatacacta tgaaaaaaat aatactactt
      661 tttggactat taaccataag tcctctgtgg gctgacggac atgaaaaaga ggttttagac
      721 cttgtctata agcactggga agcaagagat aatagtgatt ttgcaactca ggctgacctc
      781 atgagtcaat ctggaactta taacgctaat tctgatggaa gtttttttag gttttcaaaa
      841 cagaatagtg cggcagacat ggaaaaaaac atgggagatc agtcttctag tttgaatgtc
      901 atgtatccag aaaccattgc cttggctgat aatgttgttt tagctagata ttatcttgaa
      961 ggagtaataa gtgaccccgg tggaactgtt tcaaactata gaacacgagt aactcatatc
     1021 tgggttaagg aaaaaggagg gtggaagact aaatcttggc atttttcacc acttcatgat
     1081 ggaggcagac atctcacttc tgcgagtgat tttgaagagg aatagtttct atataaaaaa
     1141 ctaggagtaa tcaaggatga aaaaaatatt atttatcaca tcattagcat gtatcttttt
     1201 tgctaataat atctttgctg tagaaatgga cgtttatgtc tataaagtaa agccaggttc
     1261 tttagaggaa gctctagaga tatttcaaga aggttcatct atcgcttcac aaacaggaat
     1321 gaatgtagct atagctcaac aatcgtcagg aaaaggtggg gaattaacat accattggtt
     1381 ggaattctat gattcattag agcaacgagc cgagattcgt tatgctgacc caaaatggga
     1441 tgcatatact gaaaaattct acagttcaaa tgcaatagac acagtcagga catatagtat
     1501 gacttcccta gatgaggata ttggtgatga aaatgttgcc ttagcaagag tttatgtatg
     1561 ggatgcaaaa ccgaatatgt atgatgaaac aatcacagca ttattccagg caaaagaaat
     1621 ttttgaaaaa catggctttg ttgttgatct ttggcagcag ggagttggaa gtaagcatta
     1681 tcttgagttt gtgatgcttt ctagctctga agaagagcaa gcaaagtctc tgcgatcagt
     1741 aaatgctgat caggattgga ttgagattga gccagtgtgg atgaagaatg aagaagttgc
     1801 tcgttttatt actagttttg agcttagtaa gatcaattaa aatattttct gtgtagtaaa
     1861 acttttgcta catctatctt gggagagcaa ttttcaatta gacagatgac agaactgatt
     1921 tcttataaaa agcatcattg agtggtgcaa ttaattaata catagaggag aggaaatgaa
     1981 atattttaat ctagtgcttt ttacagtcat tcttagtttt aatctatcag cagcacacca
     2041 cgatgaagac gaacaaaaag gtattagtgg attaatctca tcagaggtct ttgatttggc
     2101 tggtgagatg gatttgtatg ttgagaggta ccagagctgt ggagatacta acaacgaaaa
     2161 gcattatcac cctgtgggaa ccttggttta tatgcttgat ggcaaggctg cctcaatatc
     2221 atctggtgaa tggaaagaat attcagaagg atcgtattgg tttgaaccct ctatgtgggt
     2281 gcatggtggc tctatgactg atgaaccaaa atttggtgat gatcagtgta gacagacatt
     2341 agttattaga gcttcaagaa aaggtgaaga accaacggta tttgttaaat aattaaagag
     2401 gagacacatg aaagttttta aaaaatattt tactactatc gggcttttaa tattttcggc
     2461 ccaattaata gctggtccag gattagctgt gaccacgatc aatacaaaag atgctgttac
     2521 ttatactaaa tggcttacag aaagcactcc aaaatttatg gaagtctatg gagaaacagt
     2581 agcatctaca ggaatttgta gtcctatttc aggtgcagaa caagaaggcg atcattacgc
     2641 ttgggcaatt gcaccctcaa tgagtgctct actgcagtct gaagtaagat tctggaatga
     2701 caaatcaatt gctaaaacaa tcaataaaat ttcttctaaa agaaatattg taagaaaaga
     2761 tatttatgaa atcataaaac ctgcaaaaag agcttataaa gttggtgaaa ccacagctca
     2821 atataatcta ttatcaagac ctaataatgt ggcagagtat gtaaaaacga ttgaggcaat
     2881 ggaagcggct gctgccgaaa atggctttga agatattcaa atggttgttt ttgaaggctt
     2941 cggtgctgga gattgggctc gtatggtgat ggcaagtatt caggcgccta atgtagagag
     3001 gttgggagct t
//
LOCUS       DASCTH010000068         2315 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000098435, whole genome shotgun sequence.
ACCESSION   DASCTH010000068 DASCTH010000000
VERSION     DASCTH010000068.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000098435"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="QGH35_01410"
     CDS             complement(<1..414)
                     /locus_tag="QGH35_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJL81225.1"
                     /translation="MTKEYNASDIEVLSGLEPVQKRPGMYTDTDCPNHLAYEVIDNSV
                     DEAIAGHCKKIEVILHNDGSLQVKDDGRGMPVDDHPEEKIPAVEVILTRLHAGAKFSN
                     KNYRFSGGLHGVGVSVVNALSKNLEAWIKRNGHEYN"
     gene            complement(492..1001)
                     /locus_tag="QGH35_01415"
     CDS             complement(492..1001)
                     /locus_tag="QGH35_01415"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJL81226.1"
                     /translation="MHGVGEKFPEFSMTAVDKNNEMIEVTSGDLNGWSVIYFYPKDFT
                     FICPTEIAEMDRLTDEGVHVIGISGDNEFCKQNWKQTNDLIKDVRHPLAADCGLKLST
                     ELGVVDADEGVCLRATFILNSDGVIQHVTVNALDTGRSADETIRTLKALQAGGLTGCS
                     WNPGDEFVA"
     gene            1165..1395
                     /locus_tag="QGH35_01420"
     CDS             1165..1395
                     /locus_tag="QGH35_01420"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HJL81227.1"
                     /translation="MSKGKDQKKSIEKSLTELERLVEELENGDLELDQALKKFEQGIK
                     LSRECQQTLEETEMKIKILMENALKTTDEELN"
     gene            complement(1387..1869)
                     /locus_tag="QGH35_01425"
     CDS             complement(1387..1869)
                     /locus_tag="QGH35_01425"
                     /inference="COORDINATES: protein motif:HMM:NF016489.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HJL81228.1"
                     /translation="MKQSLKDVYTNPSLFIATGFGVGLIPWAPGTWGSLVGVFIYLGI
                     HAQGGSLITELAIIILLFFIALWACHAVSSKYDNHDHPSIVVDEMIAMMLLLLSVPKT
                     AMWIAISFIVFRILDILKPWPVSVIDANFRSGIGVMLDDSVAALMSGLLIYVAQQFLV
                     "
     gene            complement(1873..>2315)
                     /locus_tag="QGH35_01430"
     CDS             complement(1873..>2315)
                     /locus_tag="QGH35_01430"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJL81229.1"
                     /translation="FPSSDLTGIDLTEEMLAIAKQKLSSRVELLLGSVTNLPFDSESF
                     DWVIMSNIIGHLSDKKTALLEAYRVLKNPGRIVITDWTRDFQSMNLVHFYTKLINYSQ
                     YRSLKTVELENLLDECGFNHLYTERYKINWFWGLYTILSEKNKA"
BASE COUNT          760 a          483 c          374 g          698 t
ORIGIN      
        1 gttatattca tgtccatttc gcttaatcca ggcttcaaga ttttttgaaa gtgcgttgac
       61 tacggacacg cccaccccat gtagcccacc tgaaaatcga tagtttttat tagaaaattt
      121 ggcacctgca tgcagtcgag tcagaatcac ttcaacggca ggaatttttt cttcagggtg
      181 atcatcgaca ggcatccctc tcccgtcatc ctttacttgc aaagaaccat cgttatgtaa
      241 aattacttca atttttttac agtgtcctgc tattgcctca tccacgctgt tgtcgatcac
      301 ctcataggca aggtgattgg gacaatctgt atcggtatac atcccaggtc ttttttgaac
      361 cggctcgagt ccacttaata cttcaatatc tgaagcgtta tattcttttg tcatggttct
      421 caattattct cgaagtacga gaatcaatca agatcatttt taaaagagtt tttttaaatt
      481 agtcttatgc tttatgcaac aaattcgtcg ccgggattcc atgaacaacc agtaagaccg
      541 cctgcctgaa gagctttgag tgttctaatg gtttcatcag cacttctacc agtatctaat
      601 gcattgactg ttacatgttg aatcacaccg tcactattta aaatgaaagt tgctctaaga
      661 caaacacctt catcagcatc cacaactcca agttcggttg ataattttag accgcagtct
      721 gctgctagtg gatgcctgac atctttaatg agatcatttg tttgtttcca gttttgttta
      781 caaaactcat tatcgccact aatgccaata acatgaacac cctcatcagt gagtctatcc
      841 atctcagcta tttcagttgg acaaatgaat gtaaaatcct tcggataaaa ataaatcact
      901 gaccacccgt ttagatcacc agatgtaacc tctatcattt cgttattctt atctaccgct
      961 gtcatagaga attctggaaa tttttcacca acaccatgca taatttaact ccataaagat
     1021 tttattgatt agaaccgttc taattctaac tcagatagca taccgcgaca atactttgtc
     1081 tcaatttctt taattgattg acgatactta aagtctgtgc aaatctatta ctccattatc
     1141 atcaaatcaa ttggatagag ctcaatgagt aaagggaaag atcagaaaaa atcgattgaa
     1201 aaaagcttaa ctgaacttga aaggctggtt gaggaacttg aaaacggaga ccttgaatta
     1261 gatcaagctt taaaaaaatt tgaacaaggg attaaattat ccagagaatg tcagcaaact
     1321 ttagaagaaa cagaaatgaa aataaaaatc cttatggaga atgcccttaa aaccacagac
     1381 gaggaattaa actagaaact gctgagcgac ataaattaat agaccactca ttaaggcggc
     1441 tactgaatca tctaacatta caccaatgcc gcttctgaaa tttgcatcaa ttactgagac
     1501 aggccaaggt tttaaaatat ccaatattct gaaaacaata aatgaaattg ctatccacat
     1561 agctgttttt ggcacgctta ataacaacaa catcattgca atcatctcat caaccacaat
     1621 actggggtga tcatgattat catatttact tgaaacagca tggcaagccc aaagggcaat
     1681 aaaaaacaat agaataataa ttgccagttc cgtaatcagt gagcctcctt gcgcatgaat
     1741 accaagataa atgaaaacac ccacaaggct gccccaagtg ccaggtgccc aaggaattaa
     1801 gccaacacca aatccagttg ctataaatag agatgggttg gtataaacat cttttaaact
     1861 ttgtttcatt ggttaagctt tattcttttc tgacagaatt gtatagagtc cccaaaacca
     1921 atttatctta tatctttccg tatacagatg attaaaacca cattcatcca gaagattctc
     1981 taattcaacc gtctttaaac ttctgtattg tgaatagtta atcaattttg tatagaaatg
     2041 aactaaattc attgattgga aatcacgtgt ccaatcggta atcacaattc tccccgggtt
     2101 ttttaacacc ctataagcct ctagcaacgc tgtcttctta tctgaaagat gaccaatgat
     2161 attagacata ataacccaat caaatgattc tgaatcaaag ggaaggtttg taacgctacc
     2221 taaaagaagc tcaactctgc tggaaagctt ctgttttgca atcgctagca tttcttcagt
     2281 caaatcaatt ccggtgagat cagatgatgg aaaac
//
LOCUS       DASCTH010000069         3554 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000099008, whole genome shotgun sequence.
ACCESSION   DASCTH010000069 DASCTH010000000
VERSION     DASCTH010000069.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000099008"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="QGH35_01435"
     CDS             <1..452
                     /locus_tag="QGH35_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81230.1"
                     /translation="QDNLKFNFHLHQTKTDNWFIWTDVNQYASYESKNTRISTDMKWY
                     PSERQEFQIKLQAVAFRNQQGQSWVADEAGYLNSTGAAVESINLGKIAFQIRYKYEIA
                     PLSNLYLVYTRGGDYYFDDEAGTSKIYRKTWDNPENNKLVLKLRIKF"
     gene            complement(474..881)
                     /gene="mce"
                     /locus_tag="QGH35_01440"
     CDS             complement(474..881)
                     /gene="mce"
                     /locus_tag="QGH35_01440"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HJL81231.1"
                     /translation="MIGKLNHIAVAVPDLEEATSIYRDVLGAKVSKPHELPEHGVTAV
                     FVHLENTKIELIQPLGDQSPIDSFLKRNTKGGIHHLCYEVEDIFAAKDQLTSQGMTVL
                     GTGEPYLGANNKLVLFLHPKNFMGSLIELEQAN"
     gene            complement(878..1876)
                     /gene="meaB"
                     /locus_tag="QGH35_01445"
     CDS             complement(878..1876)
                     /gene="meaB"
                     /locus_tag="QGH35_01445"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HJL81232.1"
                     /translation="MEASEIIDLVDGIKSGNRKAIAQAITLMESIKTDDRANAEALLE
                     QLGTANESSIRIGISGTPGVGKSTFIEALGQLILKEGNRLAILAVDPTSSISGGSILA
                     DKTRMTSLSQDENVFIRPSPSGNNLGGVNRSTRESIAIVEAARFNVTLVETVGVGQSE
                     TEVFEMTDIFIVLLNPGGGDELQGIKRGIIELSDIIVVNKSDGDLESLSRSTVLDYKN
                     ALKLMKPRIKDWSVPIIACSALKKRGLEKCWQSIQNLRELLLISHQLESNRNQQKEKW
                     LIKETRQGLLDALDEDEEIQNKLKTMQEKLSEENRSVKTEAKNIIANFLSKLSKNK"
     gene            complement(1878..>3554)
                     /locus_tag="QGH35_01450"
     CDS             complement(1878..>3554)
                     /locus_tag="QGH35_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014805.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HJL81233.1"
                     /translation="IIFIGPKPQAITSMGDKIQSKIIAEEAGVNVIPGFTEALQNSKQ
                     AVEVANEIGYPIMLKASAGGGGKGMRIAFDDQQCTDGFIRAQNESLASFGDNRVFAEK
                     YITEPHHIEIQILADQQGHTIYVGERECSIQRRHQKVVEEAPSPFIDNNTRIAMGEQA
                     VTLAKAVDYESAGTVEFIVDKNQKFYFLEMNTRLQVEHPVTEMTTGLDLVEEMIRIAS
                     GEVLKFKQEDLQVKGSAIETRIYAEDPYRDFMPSIGRVSKYCVPDHLDFIRIDTGIEE
                     GSDVSIFYDPMIAKLIAYGEDREKSIDRMLLALDQFHISGISHNINFLSSIMQRKKYR
                     EGNTTTNFIQEQFPDGFQGEIASENETLIVASVLACIHQNSIERYQTAIATETKKSSW
                     SVSFNKKKIDLIIKKSSKTTEILIDSSIFIIDLASTPNNPIVKARVNDDIYYFQLENL
                     HPGYRLTYRGRVLEALVTRNRTAELNQLMPEKIAPDLSKFLLSPMPGLLIKICVKEGQ
                     HVKAGEELAIVEAMKMENSLRAIDDVIIKSIFTKEGDNLMVDQTILEFED"
BASE COUNT          974 a          718 c          644 g         1218 t
ORIGIN      
        1 cacaagataa tttgaaattt aattttcatt tgcaccagac caaaactgat aattggttta
       61 tttggacaga tgtaaatcaa tatgcaagtt atgaatcaaa aaatacaaga atctctactg
      121 acatgaaatg gtatccaagt gaacgtcaag aatttcaaat aaagttacaa gctgttgctt
      181 tcagaaatca acaaggacaa agctgggtag cagatgaagc tggttatctc aatagcacgg
      241 gagcagctgt agagagtatt aatttgggaa aaattgcttt tcaaattcgt tacaagtacg
      301 agattgctcc actgtcaaac ctttatctcg tctacactag agggggtgat tactattttg
      361 atgatgaagc aggcaccagt aagatctatc gtaagacgtg ggacaatccc gagaacaaca
      421 aactggttct aaaattacgt atcaagttct aatctattgt ttactggttg caactaatta
      481 gcttgttcaa gttcgatcaa tgatcccata aagttttttg gatgcaagaa cagtacaagt
      541 ttgttattgg ctccaagata gggctcaccc gtacctaaaa ctgtcattcc ttgactggtc
      601 aattgatctt tggcagcgaa gatgtcttca acctcataac acagatgatg aatgccacct
      661 ttagtatttc tctttaaaaa actatcaatt ggtgattgat ctcccaatgg ttgtatcaat
      721 tctattttag tattttccaa atgaacaaaa acagcagtta cgccgtgttc agggagttca
      781 tggggttttg aaacttttgc tcccaataca tctctataga ttgatgtggc ctcttctaga
      841 tctggtaccg caacagcaat gtgattgagt tttcctatca tttgttttta ctcagcttac
      901 ttaaaaaatt ggcaatgata ttttttgctt ctgtctttac cgaccgattt tcttcagaga
      961 gtttttcttg catagtcttt aatttattct ggatctcttc atcttcatca agcgcgtcaa
     1021 gtaatccttg tctggtttct tttatcagcc atttttcttt ttgctggttt cgattgcttt
     1081 ccagttggtg actgattaat aacagttctc gtaaattctg gatggactgc cagcattttt
     1141 caagtcctct ttttttaaga gcagaacaag caataatagg cactgaccaa tctttaattc
     1201 ttggtttcat cagttttaag gcatttttat agtccagaac tgtgctcctt gataaagatt
     1261 ccagatcacc atcagattta ttgactacaa tgatgtcaga taattcaatg atgcctcttt
     1321 taatcccttg caattcatct ccaccacccg ggttcaggag tacaatgaaa atatcagtca
     1381 tttcaaacac ttctgtctca gattgtccga ctccaacggt ctccaccaga gtgacattaa
     1441 atctggctgc ctcaacaatg gcaatagact ctcttgtgga gcgattcaca ccgcctaaat
     1501 tgttgcccga aggcgaaggt ctaatgaata cattttcatc ttgactgaga gaggtcattc
     1561 ttgttttatc tgccagaatg gaaccgccac tgatggatga tgtgggatcg actgccaaaa
     1621 tagccaatcg attaccttct tttaaaatca gttgcccaag cgcttcgatg aaggtggact
     1681 tacccacgcc tggagtacct gaaatgccaa ttcttatgga agattcattg gcagtaccta
     1741 gttgttccag caatgcttcg gcattggcac ggtcgtcggt ttttattgat tccatcagag
     1801 tgatcgcttg tgcaatcgct tttctgttgc ccgactttat tccatcaacc aagtcaatta
     1861 tttcagatgc ttccaattca atcctcaaat tcaagaatag tttgatctac cattagatta
     1921 tcgccttctt ttgtgaatat cgattttatg attacgtcat caattgctcg caagctgttt
     1981 tccattttca ttgcttcaac tatagctaat tcttctcctg ctttgacatg ttgtccctcc
     2041 ttcacacaaa tttttattaa aagtccaggc atcggggata ataagaattt tgataggtct
     2101 ggtgctattt tctcaggcat caactggttt agttctgcag ttctgttgcg tgtaaccaag
     2161 gcttccagaa cccgaccacg gtaggtcaat cggtagccag ggtgtagatt ctcaagctga
     2221 aagtaataaa tatcatcatt aaccctagct ttcactatcg ggttattggg tgttgaggct
     2281 aaatcaataa tgaagatact tgagtcgatc aggatttctg ttgtttttga tgatttctta
     2341 atgattagat caattttttt cttgttgaaa gaaaccgacc atgaagattt tttagtctca
     2401 gttgcgatgg cagtttggta tcgttctatg ctgttttgat gaatgcaagc caacaccgag
     2461 gcgacaatga gcgtttcatt ttcactcgct atttctcctt gaaatccatc cggaaactgt
     2521 tcctgtataa aatttgttgt ggtattgcct tctcgatatt tctttctctg catgatagag
     2581 cttaaaaagt ttatattatg agaaatacct gaaatatgga attgatccag agcaagtagc
     2641 attctatcaa tacttttttc acgatcttcc ccataagcaa tcagttttgc aatcatcgga
     2701 tcatagaata tggatacatc actgccttct tcaatacctg tatcgattct gatgaaatcc
     2761 aaatgatctg gaacacaata tttactcact ctaccgatag atggcataaa atcccggtag
     2821 ggatcctctg cataaattct cgtttcaatt gctgagcctt tgacttgtag atcttcttgt
     2881 ttaaatttca gaacttctcc tgaagcaatc cggatcattt cttctactag gtcaagcccg
     2941 gtagtcattt cagtgactgg atgttcgact tgaagccttg tattcatttc aagaaagtaa
     3001 aacttttgat ttttatcaac aatgaattca acagtgccag cagattcata atctacagct
     3061 ttggccaatg tcacagcttg ttcacccatt gctattctag tgttattgtc aataaaaggt
     3121 gatggcgctt cttccactac tttttggtgt cttctttgta tggagcattc tctttcaccg
     3181 acataaatag tatggccttg ttggtcagcc aagatttgaa tttcaatatg atgaggctcg
     3241 gtaatgtatt tttctgcaaa aactctgtta tctccaaaac ttgctaaact ttcattttga
     3301 gctctgatga atccatctgt gcattgttga tcatcgaagg caatacgcat tcctttacca
     3361 ccccctccag cactcgcttt taacattatt gggtaaccga tttcattagc aacttcaaca
     3421 gcttgttttg aattttggag tgcttcagtg aatcctggta tgacattaac acctgcttct
     3481 tcggcaatta tttttgattg tattttatct cccatactgg ttattgcttg gggcttaggg
     3541 ccgataaata taat
//
LOCUS       DASCTH010000070         4894 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000099753, whole genome shotgun sequence.
ACCESSION   DASCTH010000070 DASCTH010000000
VERSION     DASCTH010000070.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000099753"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="QGH35_01455"
     CDS             <1..508
                     /locus_tag="QGH35_01455"
                     /inference="COORDINATES: protein motif:HMM:NF024786.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HJL81234.1"
                     /translation="GGELMQLVAVIQKVKELGFKDVCITGGEPLAQNDNTKHLLKELA
                     DLGFEVSLETGGSISVAGIDERVKIIMDIKSPDSGEVANNLWKNIELLKASDELKIVI
                     CSREDYLWSRDIIDQYHINEKCQILLSPCSGTLEPRQLAEWILEDHIPARFQMQIHKI
                     LWNNQPGK"
     gene            513..1202
                     /gene="queC"
                     /locus_tag="QGH35_01460"
     CDS             513..1202
                     /gene="queC"
                     /locus_tag="QGH35_01460"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019604178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HJL81235.1"
                     /translation="MTKAVVLLSGGMDSATALAVAKTDGCDCYALSIDYGQRHKYELT
                     AAKVIADDLGVIEHKIITIDLSAFGGSALTDQNLPIPVEESTGIPITYVPARNTIFFS
                     LALAWSETIEAELIYSGINAIDYSGYPDCRPEYIVALQDLFDLATKKTVEGHKIHLKT
                     PLIDLTKTEIISLGRSKGVDFSKTVSCYQTDEDGRSCGICDSCRLRIKAFKDLQIEDT
                     IEYVKRNSTVK"
     gene            1257..1332
                     /locus_tag="QGH35_01465"
     tRNA            1257..1332
                     /locus_tag="QGH35_01465"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1290..1292,aa:Lys,seq:ttt)
     gene            1445..1816
                     /locus_tag="QGH35_01470"
     CDS             1445..1816
                     /locus_tag="QGH35_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HJL81236.1"
                     /translation="MKESINVFGEPLRECSNSPMTGFFRNGCCDTSDEDFGSHTVCII
                     VTKEFLLFSASVGNDLSTPMPAMGFSGLNPGDQWCLCAPRWQEALEAGAAPKVLLSAT
                     HQGALEYIDIKYLKQFAIDLN"
     gene            1896..2798
                     /locus_tag="QGH35_01475"
     CDS             1896..2798
                     /locus_tag="QGH35_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81237.1"
                     /translation="MKTRLFLSLAFVFLMQGFFSLQADYSDQAERLDALTPDNRIIAE
                     DLMSFMKASDDQYFSWVETINKQSITDENFDWITEYSDFNVKVMRGDVVEKIGRMLSE
                     GKLTAPGRGNKDLVWGRFYSIDIHPKTPLVGMLHATIVLQFFEDNSVATGGWLDMMPG
                     TRIPEDLEFLRQVTDNYFAKNGANPSLYRRLVCKGTEDTIEYWRRKPSCSGVSFYGPP
                     VFRESGEKSYQFIKGMFTEFVDAYLQLVERRMNDRFTNEDLLAQEEMRKRWLIDQLFS
                     DPYASKIVPFEVWSTANMPPTVRF"
     gene            2798..3391
                     /locus_tag="QGH35_01480"
     CDS             2798..3391
                     /locus_tag="QGH35_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81238.1"
                     /translation="MENSIKIDNEYEDPNRFFWYFIYASITIVTALPLLGLRISDFGI
                     NYLFLLVVHEMAAFLFFGHTLFSNIWAMQIRFNQSTEVGVWARAFLRKLALSITMTTS
                     IIIPVVGLMLIETWGGLHNAAWAWNAYLAFWIMGAVSITPDVIRYGRNRNAGDPKHGM
                     ASGLIRGNIALLLTIYIFLCMVVKIAWVTPFPKLTIF"
     gene            complement(3422..4384)
                     /locus_tag="QGH35_01485"
     CDS             complement(3422..4384)
                     /locus_tag="QGH35_01485"
                     /inference="COORDINATES: protein motif:HMM:NF025587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HJL81239.1"
                     /translation="MKKLSFKNFQIDIDSKAAQREIDLLKEALYANEVLILIDHKIPK
                     EIIDNAYEQSKIFHSLDDDDPRKQSAHYRNAHFGRGWSPCGQEPAYLANTKATCSAFD
                     LCYEITEIDHEHENYGPNLWPPKMPEFRKHVYNYYLTFTALEQTFARALEHALDLEKD
                     YITSKMTERSPSTMRLIYYPCVEDEPEANLYGISAHTDYEVFTLLTQTNLGLEIQNQK
                     GEWLGVDVGPYEVVVNIGDMIERMTNGKIKASNHRVVPVQWIRYSIARFCAIDGDHIV
                     KPLSQFVDPDIGPLYEPITQQDNIKNGLAQATANSEAMKQQAVN"
     gene            4506..4793
                     /locus_tag="QGH35_01490"
     CDS             4506..4793
                     /locus_tag="QGH35_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013851.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HJL81240.1"
                     /translation="MDKEIIKIKKTLNQSMDISILEVEDQGHLHQGHKAAVEGKLHLK
                     MIIVSDEFINLNPVERHQNIYSILADFLAGRLHALSLETYTIKEYSNLREI"
BASE COUNT         1497 a          799 c         1005 g         1593 t
ORIGIN      
        1 tgggggtgaa ttgatgcaac tagtagccgt tattcaaaag gtaaaagaac ttgggtttaa
       61 agatgtgtgt attacaggcg gagaaccttt agctcaaaat gataatacaa aacatcttct
      121 taaagaactg gctgatttgg ggtttgaggt cagtcttgaa actggaggtt caattagtgt
      181 tgcaggcatt gacgagagag taaaaataat tatggatatt aagtcccctg attccggtga
      241 agtagctaat aatctctgga aaaatataga gttattaaaa gcatcagatg aattaaaaat
      301 agtcatttgc agtagggagg actatctttg gtctagagat atcattgatc agtatcatat
      361 caatgagaaa tgtcagattc tcttatctcc atgttcaggg acattagagc caagacagct
      421 tgcagaatgg attttagagg accatattcc agcaagattc caaatgcaga tacataagat
      481 tttatggaat aatcaaccag gaaaataaca ttatgaccaa agctgttgtt ttattgtcag
      541 ggggaatgga ttctgcaaca gcattggctg tggcaaaaac agatggttgc gattgttatg
      601 ctttgagtat tgattacggc cagagacaca agtatgaatt gactgcagct aaagtaattg
      661 ctgatgattt aggtgttatc gaacataaaa ttattactat tgatttaagt gcttttggtg
      721 gttcagcatt aactgatcaa aatttaccaa ttcctgtaga ggaatccact ggtataccta
      781 tcacttatgt tccagcaaga aatactattt ttttctccct agccttagca tggagcgaaa
      841 ctatagaagc ggagctaatt tactctggaa tcaatgcaat tgattactca ggctatcctg
      901 attgcaggcc tgaatatata gttgctcttc aagacttatt tgacctggcc actaaaaaaa
      961 ctgtagaggg acataaaatc catttaaaaa caccattgat agatctcaca aagactgaga
     1021 ttatttcttt gggaagaagt aaaggtgttg atttttcaaa aacagtttct tgttatcaaa
     1081 ccgatgaaga tggaagatct tgtggaattt gtgattcgtg taggctcagg attaaggctt
     1141 ttaaagactt gcaaattgaa gatactattg aatacgttaa aagaaattcg actgttaaat
     1201 gagtttttta tttatttgcc ttgaggtatt attcaaccgc atttcatact tttaccgggc
     1261 cgttagctca gtcggtagag cagctggctt ttaaccagtt ggtcgaaggt tcgaatcctt
     1321 cacggcccac cagcttgaat agatgaatcg aaatacaaga aaatactgga aggtctctgc
     1381 tgttacagca atggtttttt ttgtaatact agttctaatc tttattaatt cattgtaaat
     1441 ttttatgaaa gaatcaatta atgtttttgg agagcctctc agagaatgta gcaactctcc
     1501 gatgacaggt ttttttagaa atggatgctg tgacacctct gatgaggact ttggcagtca
     1561 tacagtttgt ataattgtta caaaagaatt tctattattc tctgcctcgg ttggtaatga
     1621 tttatccaca cctatgcctg caatgggatt ttcaggttta aaccctggag accaatggtg
     1681 tctctgtgcg ccaagatggc aagaagcttt ggaagctgga gccgctccaa aagttttatt
     1741 gtctgccact catcaagggg cacttgaata cattgacatc aagtatctga aacaatttgc
     1801 cattgattta aactaagttt taaaagcaag ctgtaatttt gatttagaat ataataaaaa
     1861 ttaatacgat tggatgaatg attgagaaga ggtaaatgaa aactagacta tttttatcac
     1921 ttgctttcgt ttttttaatg caaggatttt tttctttgca ggctgattac tctgaccaag
     1981 cagaacgatt ggatgcattg acacctgata acagaataat tgctgaggat ttgatgtctt
     2041 ttatgaaggc atccgatgac caatattttt cttgggtaga aacaatcaat aaacaatcaa
     2101 ttaccgatga aaactttgat tggattactg aatattctga ttttaatgtc aaagttatga
     2161 gaggagatgt ggtagagaag ataggaagaa tgctatcaga gggtaaattg actgccccag
     2221 gaagggggaa taaagatcta gtttggggtc gattctattc tatagacatt catccaaaaa
     2281 cacctttagt gggaatgtta catgcaacta ttgttttaca attctttgag gacaattctg
     2341 tggctactgg tggatggctt gacatgatgc caggaacaag aattcctgag gatctggaat
     2401 ttttaagaca ggttaccgat aattattttg caaagaatgg tgcaaaccca tccttatacc
     2461 ggagattagt ttgcaaagga actgaggaca ccatcgagta ttggcgcagg aaaccttcct
     2521 gttctggtgt gagtttttac ggtccacctg tctttaggga aagtggtgag aaaagctatc
     2581 aatttattaa agggatgttc acggaatttg tggatgctta tttgcaactg gttgaaagaa
     2641 ggatgaatga tagattcacc aatgaagatc tcttggccca agaggaaatg cgaaagcgct
     2701 ggctaattga tcagctattc tcagatccat atgctagtaa aattgtacct ttcgaggttt
     2761 ggtcaactgc caatatgcct ccaacagtaa gattttaatg gaaaatagta taaaaataga
     2821 taatgaatat gaggatccta atagattctt ttggtatttc atctatgctt ccataaccat
     2881 tgttactgct ttacctttac tgggattaag gataagtgat tttggaatca attacttatt
     2941 tttgcttgtg gtccatgaga tggcggcatt tttatttttt ggacacacat tgttttctaa
     3001 tatttgggct atgcagattc gttttaatca atcaactgaa gtgggtgttt gggcgagagc
     3061 atttttaaga aaacttgctc tatcaattac catgactacc tcaattatta tcccagttgt
     3121 gggactcatg cttatagaaa cgtggggagg gcttcataat gcggcttggg catggaatgc
     3181 ttatttagca ttttggatta tgggagccgt aagtattact ccagatgtta ttagatatgg
     3241 aagaaacagg aatgctggtg accctaaaca tgggatggcc agcggtttaa ttagagggaa
     3301 tatagcttta cttttaacca tctatatttt cttgtgtatg gttgtaaaga ttgcttgggt
     3361 gacgcccttt ccaaaattaa ctatatttta aattcttaga aagaaacccc ttaattagtt
     3421 gctaattcac tgcttgctgt ttcatcgcct cagaatttgc tgtagcttgg gccaatccgt
     3481 ttttgatatt atcttgttga gtgatcggct cataaagagg tccaatatct ggatcaacaa
     3541 attgagacag gggtttgaca atgtggtctc catcaatggc acaaaaccga gcaatagaat
     3601 agcgtatcca ctgcacagga acgactctgt gattgctggc ttttattttt ccattagtca
     3661 tgcgttcaat catatctcca atattgacga caacctcata aggacctacg tcaacaccaa
     3721 gccattcgcc tttctggttc tgaatttcta gtcctaagtt cgtttgtgtc agcagtgtaa
     3781 agacttcata gtctgtatga gcgctgattc cataaaggtt tgcttcaggt tcatcttcta
     3841 cacagggata gtaaattaac ctcatcgttg aaggggatcg ctcggtcatt tttgatgtta
     3901 tgtaatcttt ttcaagatca agtgcatgtt caagggctct tgcaaatgtt tgttctagcg
     3961 ctgtaaatgt taggtaataa ttataaacat gtttcctgaa ttcaggcatc tttggcggcc
     4021 acaggttggg accataattt tcatgctcat gatcaatctc tgtaatctca taacataaat
     4081 caaaggcaga acacgtggct tttgtatttg ctaaataagc tggttcttgt ccacacggcg
     4141 accatcctct cccaaagtga gcgtttcggt aatgagccga ctgttttctt ggatcatcat
     4201 catccaagct gtgaaagatt ttagattgct cataagcatt gtcaataatc tcttttggta
     4261 tcttgtgatc aattaagatc agaacctcat ttgcatacaa ggcttctttg agaagatcta
     4321 tttctctttg tgcagctttg ctgtcaatgt cgatttgaaa atttttaaaa ctgagttttt
     4381 tcaatatgtg tttcactcat acttattgca attagagact atataatact ttaatataaa
     4441 tactatataa cgattacgga attgctttta tttagatcat gatagcggtt ctagagggag
     4501 taaagttgga caaggaaatc attaaaatta aaaagacact caaccaaagt atggatatat
     4561 caatacttga agttgaagat caaggtcact tacatcaagg acataaagct gctgtggagg
     4621 ggaaactgca tctaaaaatg atcattgttt ctgatgaatt tataaattta aatcctgttg
     4681 aaagacatca aaacatatac tctattctgg ctgattttct tgctggtaga ctccatgcat
     4741 taagtcttga aacatacact atcaaagaat atagcaattt gagggagatc taatttttaa
     4801 tggcaatgac accggcatgg tctttagcat ctttgtcctc attatgagaa aaacctagaa
     4861 aggttgattc aagaatttta aatccagcat ctct
//
LOCUS       DASCTH010000071         1877 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000102168, whole genome shotgun sequence.
ACCESSION   DASCTH010000071 DASCTH010000000
VERSION     DASCTH010000071.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000102168"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            39..521
                     /locus_tag="QGH35_01495"
     CDS             39..521
                     /locus_tag="QGH35_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJL81241.1"
                     /translation="MTIAIGDQIPNIELTTMNKEGPSAVTYEDLFANKKVALFAVPGA
                     FTPTCSQQHLPGFIGQAQQLKEKGIDIVACMSVNDVFVMNAWGKSHGADEEVLMLADG
                     NGAFTKALGLELDASGYGMGTRSQRFSLITNNGVIETLNIEEGGEFRVSSCEFMLGQI
                     "
     gene            529..1527
                     /locus_tag="QGH35_01500"
     CDS             529..1527
                     /locus_tag="QGH35_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HJL81242.1"
                     /translation="MSTNNQIILKRNPRGIPEVRDFGLINTPIPNIARNQILTKTIHI
                     SLDPYIRGLITGKHIYAEKINIGDVIAGRTIGEVIKSKNDDFNEGEIIVCSNGWQEYA
                     VTNGEGVRKLNPEQAPITTALGILGMPGLTAYAGLLVYGEPKDGDIVVVSAASGPVGS
                     MVGQIAQISGAKALGIAGSMAKCSLVKEEFGFFECINYKTENLDQRIKQLCPNGVDIY
                     FDNVGGQTLETMTNNLAMHARIVLCGFMTQYNSETTPPGPNLAPVVGARATIKGVVVY
                     DHYDKQEQFISLASKWLDEGKIKYKEDEVFGLDQAPILFCRLMRGENFGKAIVTLK"
     gene            1544..>1877
                     /locus_tag="QGH35_01505"
     CDS             1544..>1877
                     /locus_tag="QGH35_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81243.1"
                     /translation="MSVQDVTLILHLLLFAYWLGGDIGVFYSSGFAINSNHSIEARKI
                     AGKIMINLDLVPRLCLSLMLTVGGILSESYGIEHDPLFRLGIILLGPVWFSVLLYIHF
                     NEGTDLVKR"
BASE COUNT          616 a          288 c          416 g          557 t
ORIGIN      
        1 atcagataca atttagaata aaacacaagg aaaatattat gactatagct ataggagatc
       61 agatcccaaa tattgaactc acaaccatga ataaggaagg accctctgcg gtgacatatg
      121 aggatttatt tgcaaacaaa aaagtagctt tgtttgcagt tccaggagca tttactccca
      181 cttgttctca acagcattta ccaggtttca taggtcaagc tcaacaatta aaagaaaagg
      241 gtattgatat tgtggcatgc atgtcggtca atgacgtatt tgtgatgaat gcttggggta
      301 aaagtcatgg agccgatgaa gaagttttga tgcttgcaga tggaaacgga gcattcacta
      361 aagcacttgg tctagaattg gacgcttcag gatatggtat gggaacaaga tcgcagcgtt
      421 tttctttaat tactaataat ggtgttattg aaaccttaaa tattgaagaa ggaggagaat
      481 ttagagtaag cagttgcgaa tttatgcttg gtcaaattta aatatcactt gagtactaat
      541 aatcaaatta ttttaaaaag aaatcctcga ggcattcctg aagttagaga ttttggtctg
      601 atcaatacac caatacccaa tattgctagg aatcaaatac tgacaaaaac aatccatatt
      661 tctcttgatc cttatattcg tggacttatt acaggaaaac acatctatgc agagaaaata
      721 aacataggag atgtgattgc tggccgcact attggagaag ttatcaagtc aaaaaatgat
      781 gacttcaatg agggtgagat cattgtttgc tctaacggct ggcaagaata tgcggtaact
      841 aatggagagg gtgttcgtaa actcaacccg gaacaagccc cgattactac agcacttggt
      901 attctgggca tgcctggttt aaccgcttat gcaggcttgt tggtctatgg agaacccaag
      961 gatggcgaca ttgtagttgt ttctgcagcc agtggtccag tgggaagtat ggtaggacaa
     1021 attgctcaaa tatctggtgc caaagcatta ggaattgcag ggagtatggc aaagtgttcg
     1081 ctggtcaaag aagaatttgg tttctttgaa tgtattaatt ataagacaga aaacttggat
     1141 cagagaataa aacaactttg tccaaatgga gttgatattt attttgataa tgtcggaggt
     1201 caaacactgg aaaccatgac caataatctt gcgatgcacg caagaattgt tctttgtgga
     1261 tttatgacac aatataattc agaaactaca ccaccaggac cgaatttagc accggtagta
     1321 ggagcaagag caaccattaa gggtgtagta gtttatgatc actatgataa acaagaacaa
     1381 tttatttcac ttgcatccaa gtggctagat gagggtaaaa taaaatataa ggaagatgaa
     1441 gtttttggac ttgatcaagc tccaatactt ttttgcagat taatgagggg cgagaatttt
     1501 ggcaaagcca ttgttacgtt aaaataaatg agaggttaga aaaatgtcag tacaagatgt
     1561 aacacttata ttgcacttat tgctttttgc ctactggttg ggtggagata tcggtgtttt
     1621 ttattccagt ggatttgcta ttaatagtaa ccattcaata gaagcaagga aaattgcagg
     1681 gaaaattatg atcaatctgg acttggttcc cagactttgt ctgagtttga tgttaactgt
     1741 tggtggaatc ctttcagaga gttatggcat agagcatgat cctttattca gattaggaat
     1801 aatacttcta ggtcctgttt ggttcagtgt gttgctctat attcatttta atgagggaac
     1861 cgatctggta aaaagaa
//
LOCUS       DASCTH010000072         3094 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000102378, whole genome shotgun sequence.
ACCESSION   DASCTH010000072 DASCTH010000000
VERSION     DASCTH010000072.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000102378"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            93..1115
                     /locus_tag="QGH35_01510"
     CDS             93..1115
                     /locus_tag="QGH35_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HJL81244.1"
                     /translation="MTRINQRWLLNKRPEGMPEDDCWKWEEVDAPELKDGEILIKSLY
                     LSIDPYMRGRMNDAMSYTGPVQIGDVMVGESAGIVIDSKSDNFKPDDYVCVHGGWQSY
                     VAVRGDGPELFKADPDIVPLSTYLGTVGMPGRTAYFGLTRLGKPKEGETVVVSAASGA
                     VGTVVGQIAKILNCRAVGIAGGDVKCSYVTDELGFDACIDYKGGNLEQDLKQACPDGI
                     DVYFENVGGEVTKAVAKLLNPGSRVPICGFVSKYNSRDIMNEETPFDVFGTMRPKPKH
                     RFFVVTEWFDHWADATKELSGWIKEGKIKYRETVTDGLINAPQALRDVLSGKNFGKQL
                     VKVADE"
     gene            complement(1152..1766)
                     /locus_tag="QGH35_01515"
     CDS             complement(1152..1766)
                     /locus_tag="QGH35_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81245.1"
                     /translation="MKRFSVILAVLLSVLLSSCTTTSYQKLDSSVGYWDESINQSADT
                     FKIGFDGTEWNRNSAIEQEKVMDYALLRSAEVALDNGFRYFLISENHSYTEKKTTKET
                     ETSNSETFRHRGIFTTVNYVDLEEKLRHNNMMQDGEFKGYAVYNADLVLYIIKTKYNL
                     SNNKLLPPFERGMQAYRMPGTSRSMIETIDAVPHNPISLPPQDQ"
     gene            1868..2614
                     /locus_tag="QGH35_01520"
     CDS             1868..2614
                     /locus_tag="QGH35_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HJL81246.1"
                     /translation="MAGHSKWANIQHRKGRQDKKRGKLFTKLIREITVAARIGGSDLN
                     SNPRLRTAIDKAKNQSMPKENIDRAISKGTGEGEGFYLEEVTYEGYGPGGVAILIECM
                     TDNKNRAVAEVRYALSKFGGNLGTEGSVAYLFKQVGLISLSGVEDPDRIMEVAIDSGA
                     EDIEYDEGGSMDIITKPADFLNVRDSLLAQGINPEISEVIMRALNDVSIDIEHAESML
                     KLLDILEDLDDVQAIHSNAEIPNELLERLN"
     gene            2635..>3094
                     /gene="ruvC"
                     /locus_tag="QGH35_01525"
     CDS             2635..>3094
                     /gene="ruvC"
                     /locus_tag="QGH35_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HJL81247.1"
                     /translation="MKIFGIDPGSRTTGYGVIESKGNKSSYIGSGVIQTKEKNFQKRL
                     KVIFTEIEKLIVEYQPDVVSIENVFMHRNPDSALKLGQAKAAAICGTFKQGLIVFEYA
                     AREVKQAVVGSGAAEKEQVQYMVKIILGIKNKELKLDESDALAIAICHAHS"
BASE COUNT         1003 a          479 c          700 g          912 t
ORIGIN      
        1 cttgaatagt attccaagaa ataaactgcc acaagttcga aataattttt aatatttgtt
       61 ggccgataaa aattaatgtt agggagacaa taatgaccag aataaatcaa aggtggttat
      121 taaataaaag accagaaggg atgcctgagg atgattgctg gaaatgggag gaagtagatg
      181 ctccagaatt gaaagacgga gaaattctaa ttaaatccct atatctttct attgatcctt
      241 acatgcgagg acgcatgaat gatgccatgt cttatacagg accagtacag attggcgatg
      301 ttatggtggg agaaagtgct ggaattgtta tcgatagtaa atcagacaac tttaaacctg
      361 atgactacgt ctgtgtccat gggggttggc agagctatgt tgctgttaga ggtgatggtc
      421 ctgagctttt taaggccgat cccgatattg ttccactatc aacttactta ggaactgtcg
      481 gcatgccagg caggacggct tattttggtt taactcgatt gggtaaacct aaagagggag
      541 aaacagttgt tgtttctgct gcctctggag cagtcggaac ggttgtagga cagatagcca
      601 agatactcaa ttgtcgagcc gtgggcattg ctggaggaga tgtgaagtgt tcctatgtaa
      661 ctgatgagct tggatttgat gcttgtattg attacaaggg tggaaatcta gaacaagatc
      721 ttaagcaagc ctgtccggat ggtatagatg tgtattttga gaatgttgga ggtgaggtca
      781 ctaaggctgt tgccaagtta ttaaaccctg gtagtagagt tcctatatgt ggttttgttt
      841 ccaaatacaa ctcgcgagat atcatgaacg aggaaactcc tttcgatgta tttggtacga
      901 tgcgcccaaa acctaaacac agattcttcg tagtgacgga atggtttgat cattgggcag
      961 atgctaccaa agaactttca ggctggataa aagaaggcaa gattaaatac cgcgaaacag
     1021 taactgacgg acttatcaat gcacctcagg ccctaagaga tgtattgagt ggtaagaatt
     1081 ttgggaaaca gttggttaag gttgcagatg aatagttcta gatttctttc actcatgaat
     1141 gatttataat atcactggtc ttgaggcgga agactaattg gattatgtgg aaccgcatct
     1201 atcgtttcaa tcatgcttcg tgaagttcct ggcatcctat atgcttgcat ccctctttca
     1261 aaaggcggca atagtttatt attggataag ttgtattttg ttttaatgat gtagagcact
     1321 aaatcagcat tgtaaacagc ataaccttta aactctccat cttgcatcat attgttgtgg
     1381 cgtaattttt cttcaagatc aacataattg acagtcgtaa aaattcctct gtgacgaaaa
     1441 gtttcactat tagatgtttc tgtctctttg gtggttttct tttctgtata ggagtggttc
     1501 tcgctgatta aaaaatatct aaatccatta tctaatgcta cttcagcaga tcgaagtaat
     1561 gcataatcca tgactttttc ctgctcaatt gcagaatttc tattccattc agtaccatca
     1621 aatcctattt taaaagtgtc agcagattga ttaatacttt catcccagta tccaactgaa
     1681 gagtctagtt tttgatagct tgtagtagta catgaagaaa gtaaaacaga aagtaaaacg
     1741 gctaaaataa cactaaatct tttcatagaa tcaattatac aaaaacattt tacctagaca
     1801 ctatactaat cggtttattg tataattggt caggttccat tattaatgat tgcatcatag
     1861 gacttaaatg gctggacata gtaaatgggc taacatccaa cacaggaagg gacgccaaga
     1921 taaaaaaaga ggcaaacttt ttaccaaact aatacgtgaa atcactgttg cagcaagaat
     1981 tggaggcagt gacttaaatt ccaatcccag attgcggaca gcaattgata aggcaaaaaa
     2041 tcaaagtatg cctaaggaga atattgatcg agcgatttca aaaggtacag gagaagggga
     2101 aggtttttat ttagaagaag tgacttatga aggttatgga ccgggtggag tagcaatttt
     2161 aattgagtgc atgactgata ataaaaacag agcagtggct gaagtgagat atgcactgtc
     2221 taagtttgga ggtaatttag gaaccgaagg atccgttgct tatcttttta agcaggttgg
     2281 tttgatctca ttatctggag ttgaggatcc ggatagaata atggaagtag caattgactc
     2341 tggggcagaa gatattgaat acgacgaggg aggatccatg gatattatta ctaagcctgc
     2401 tgatttttta aacgttaggg attctttatt agcacaaggg atcaatccag aaatttctga
     2461 agtaatcatg agagctttaa atgatgtttc tatagatatc gaacacgctg aatcaatgtt
     2521 aaaactatta gatatactcg aagatttaga tgatgtacaa gcgatccatt caaatgctga
     2581 aataccaaac gaactattag aacgattaaa ttgaatcaat ataagaggta accagtgaag
     2641 atatttggca tagatccagg ttccagaacg acaggttatg gtgtcataga gtcaaagggc
     2701 aacaagtcta gctacatagg tagcggtgtg attcaaacca aggaaaaaaa ttttcaaaag
     2761 agattgaaag tgatatttac agaaattgag aaattgattg ttgaatacca gccagatgtt
     2821 gtttcaattg aaaatgtttt tatgcataga aatcctgaca gtgccttgaa attagggcag
     2881 gcaaaagcgg cagcgatttg tggaacgttc aaacaaggat taattgtttt tgaatacgct
     2941 gctcgagagg taaagcaagc agttgtgggg tctggtgctg cggaaaagga gcaggtgcaa
     3001 tatatggtta aaatcatctt aggtataaaa aataaggagt tgaagctcga tgaatcggat
     3061 gcacttgcaa tagcaatctg tcatgcacat agtt
//
LOCUS       DASCTH010000073         2980 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000104943, whole genome shotgun sequence.
ACCESSION   DASCTH010000073 DASCTH010000000
VERSION     DASCTH010000073.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000104943"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2820
                     /locus_tag="QGH35_01530"
     CDS             <1..2820
                     /locus_tag="QGH35_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81248.1"
                     /translation="RMGSGTFLDREAIDGIPTINRSIADIAKMDPRVSINSGSSRYSE
                     ISVMGANNKFNDFTIDGVSFNDPFGLNPNGFGSMRNPIGFEFIDQISVDVTPFDVARG
                     NTTGGSIATVTKSGSNEFHGSVFYIERDEDNIGDDPDGGEFPEFSEETMGFTFSGPII
                     EDRLFFFVGYEEFESASPSLWGTKDSNAVNKAEVLTTAMAEQIKGIAMSRYNYDPGVI
                     TGFATPETAEKTMLKLNANITDDHRAVFLYTKDEDLYPRKYNGGPTVFSNNYYLKPPE
                     TDRYTLTLYSDWNDKLSTKIRYTNYELKEDDHSIGDPFFPEVNITVSDGSGNQDNVYL
                     GGDRYRGANFINIESDFINLKATYDLGNHVITAGIDLEDTSVYNLFIARYNGEVRFPS
                     IADFEAGTYDYLRFHVPSTGISDVDSVAAIFDIEKTTIYIQDKIYLGDLMINVGVRYD
                     QIETPDKPLLNTKWVSKYGLNNNEAFDFDSIQPRVGFNWDISESVFGNVDSIIKAEVR
                     GGYGLFSGRIPNVWYSNAYTRSGGLSDYVKVKYPGYTKALSPWNCAGGDCTVGPMPAG
                     DPSFFWMGAGSNYKISSPPYMNDAQGTDPNFEAPASWRMNLALDIVTDSGYEMTFEYN
                     HDDVDAGVFYQELGLTLDSYLADGRGVYSHGAGDYYMTNTDKGGAEAYTFTVRKSFDN
                     GISFYGAMSKVDARDVYPLTSSQAESVYGYTQRWDGENMDAAASSFMLDRKIILGLEY
                     KTQLFGDNETRISALYIRKSGEPYSVTFDEPSYSPVSGTGCNGYCSSKFYADYSLAYI
                     PTGADDSNVEFASASVASAVMAHINSTALAKYKGTYAPRNAFRNSSNSRLDIRVTQEL
                     PAFMDGHKFVFYLDLLNVLNMLDDEDGRIFEYGYNNSRQIMVSGVTDDGKFKISGVDP
                     DDSLYLQDGDGQSRWQLQMGLKYRF"
BASE COUNT          878 a          552 c          674 g          876 t
ORIGIN      
        1 agaatgggat cgggaacatt tcttgatcgg gaagcaattg atggcattcc tacgatcaac
       61 agatcgattg ccgatattgc aaaaatggac ccaagggttt ctattaactc cggttccagc
      121 cgatattctg aaatttcagt aatgggtgcc aacaacaaat ttaacgattt caccattgat
      181 ggggttagtt ttaatgaccc atttggactc aatccgaatg gctttggaag catgagaaac
      241 ccaattggtt ttgaatttat tgatcagatt tcagttgacg ttactccttt tgatgtggcc
      301 cgtggaaaca caaccggtgg atccattgcc actgtaacga aatccggatc caacgaattt
      361 catggcagtg tcttctacat tgaacgcgat gaagacaata ttggtgatga tcctgatggc
      421 ggtgagtttc ctgaatttag tgaagaaact atgggcttta ctttcagtgg tccaatcatt
      481 gaagacaggc tgttcttctt tgttggttac gaggagtttg aatctgccag cccatcgctt
      541 tggggcacaa aggacagtaa tgcggtgaac aaggcggaag tgcttactac agcaatggcg
      601 gagcagatta agggcatagc aatgagtcgc tacaattatg atccaggagt cattactgga
      661 tttgcaaccc ctgaaactgc tgagaaaaca atgcttaaat tgaatgctaa tattaccgat
      721 gatcacagag cagtattttt gtatacaaaa gatgaagatc tctaccctag aaaatacaat
      781 ggtggaccca cggtatttag taacaattat tatttgaaac caccagagac tgatcgctat
      841 accttgactc tatacagcga ctggaatgat aaactatcga ctaaaatcag atacaccaat
      901 tatgagttaa aagaagacga tcactctatt ggggatccct ttttcccaga agtgaatata
      961 actgtcagtg atggcagtgg aaatcaggac aatgtttatc ttggtggcga ccgttataga
     1021 ggtgccaact ttattaatat tgagtctgat tttatcaacc ttaaggcaac ttatgattta
     1081 ggtaatcacg tgattactgc cggtatcgat cttgaagaca cctcagttta taacctcttc
     1141 attgcccgat acaacggcga agttcgattt ccaagtattg ccgattttga ggccgggacc
     1201 tatgactatc taaggttcca cgttccatct accggtatca gtgatgttga tagtgtcgca
     1261 gcaattttcg atattgagaa aaccactatc tatattcagg ataaaatcta tctgggtgat
     1321 ttgatgatca atgttggagt gcgttacgat caaatcgaga cacccgataa gccattattg
     1381 aataccaaat gggtcagtaa atatggtttg aacaataatg aagcgtttga ttttgattcc
     1441 attcaaccca gagtgggttt caattgggat atatctgaga gtgtctttgg caatgttgat
     1501 tcgatcataa aagcagaagt cagaggcggt tatggactgt tttccggacg cataccaaat
     1561 gtctggtatt ccaatgctta tacccgatca gggggcttga gcgattacgt taaagttaag
     1621 tatccgggtt atactaaagc cttgagccct tggaattgtg caggagggga ttgcaccgtt
     1681 ggtccaatgc cagctggcga tccctcattt ttctggatgg gcgcgggaag taactacaag
     1741 atttccagcc ccccttatat gaatgatgct cagggaacgg atccaaactt tgaagcaccc
     1801 gcttcttgga gaatgaatct tgctctggat atagtgaccg atagcggtta tgaaatgacc
     1861 tttgaataca accatgacga tgtggatgca ggcgtatttt atcaagaact aggattgaca
     1921 ttggatagtt atctcgcgga tggcagaggc gtttattctc atggtgcagg tgattattat
     1981 atgaccaaca ccgataaggg tggagctgag gcttatacat tcaccgttag aaaatccttt
     2041 gataacggta taagttttta tggtgcaatg tctaaagtag acgcaagaga tgtctatcct
     2101 ctgacctctt cacaagctga atcagtctat ggctataccc aaagatggga cggagagaac
     2161 atggatgctg ctgcatcgag ctttatgctg gacagaaaaa ttatactggg attggaatac
     2221 aagactcaat tgttcgggga taatgaaact agaatttctg ctctctatat cagaaaatct
     2281 ggtgaaccct acagtgtgac atttgatgag ccaagttaca gtccagttag tggcacaggt
     2341 tgtaatggtt actgttccag taagttctat gctgattatt cgcttgccta tattcctacc
     2401 ggagctgatg atagcaatgt agagtttgca agtgcatcag tggcatcagc agtgatggca
     2461 cacatcaata gcactgctct agcaaagtac aaaggtactt atgccccaag gaatgccttt
     2521 aggaattcaa gcaattccag gttggatatt agagtgactc aagaacttcc agcctttatg
     2581 gatggtcata agttcgtatt ctatcttgat ttgctcaatg ttctaaatat gcttgatgat
     2641 gaagatggta ggatatttga atatggctat aacaatagcc gtcaaattat ggtgagtggc
     2701 gtgaccgatg atggtaagtt taaaatttct ggagttgatc cagatgatag cctatacctt
     2761 caagacggtg atggtcaatc aagatggcaa ttgcaaatgg gtttgaaata cagattctaa
     2821 ggctaatttg tcataacaat aaaagccgcc tctcgtaggc ggcttttttt atttaaaatt
     2881 ccttattcat tgatagaata aaacctatgt ttggaatcaa accaaaaaaa ctaaattaca
     2941 tcgctttatt tactttgcca atagtggcgg tgatcacttc
//
LOCUS       DASCTH010000074         3133 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000105811, whole genome shotgun sequence.
ACCESSION   DASCTH010000074 DASCTH010000000
VERSION     DASCTH010000074.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000105811"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="QGH35_01535"
     CDS             complement(<1..187)
                     /locus_tag="QGH35_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81249.1"
                     /translation="MDAETIKEKANSADENITFTDDACEALTQVPDFAMDMAINHMVN
                     AAKDQGVDTIDPEFLNAN"
     gene            complement(280..1542)
                     /locus_tag="QGH35_01540"
     CDS             complement(280..1542)
                     /locus_tag="QGH35_01540"
                     /inference="COORDINATES: protein motif:HMM:NF018004.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HJL81250.1"
                     /translation="MTETGKNNLVKAFKALRPIPEKVLEVIAPPYDVISSDEARILAA
                     NKPLSFLHISRPEIDLPVSTSYDNPEVYQQGLINFKNLIDEEILVEEDIDSIYVYEIS
                     LDTHKQTGIGCIASLDAYEHNLIKKHEHTTPKKEKDRFNNIATLNAQTGPVLLAHKKN
                     DDLKNILSEITIHTLPLYDVEANDGSRHKIWRLSDANTLKSILALLNDMDALFIADGH
                     HRSAAAAKVRKNKKAANKDHNGTENYNYVLVVAFPEDEMNILDYNRVTKDANGHSKKS
                     LLQATDQFFDIETMVSTFKPTQSNQFGMYFQGNWYKLELKQTHRKAIDPVKSLDVSLL
                     HDYLLEPILGIQDERIDQRIDFVGGIRGIEALQERVDSGEMAIGFSLYPTPMNALISV
                     AEAHQIMPPKSTWFEPKLLDGLLTHLIG"
     gene            1667..2338
                     /locus_tag="QGH35_01545"
     CDS             1667..2338
                     /locus_tag="QGH35_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HJL81251.1"
                     /translation="MIPAHIQKAVSSKNRPASDQERDSLRKPGEILNFFDVKEGDKIG
                     ELNAGRGYFSSIAANTVGEHGIVIAHTSPMSVKRWKGNPIEKRLEEFPQSNLIPVVGE
                     MESPNFPGQLDKIFNIMTYHDSVWTGTNRDRMNTSVFNTLRDTGIYGILDHHAKLGRG
                     KDDCHKIHRIEKEFVIDEILRAGFILEKESNLFKNPEDDLTSMVFEKGIRDQTSRFLL
                     CFKKS"
     gene            complement(2365..2970)
                     /locus_tag="QGH35_01550"
     CDS             complement(2365..2970)
                     /locus_tag="QGH35_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81252.1"
                     /translation="MEENRKQYNTDNKKDGPPVSAMKPENLAPPTGMEGREADLAAAM
                     QVLVKTIKMQMPYPHDTNDALLKAHLNTIQFAKNKGLLEELVAHDLETMKPLLERSKA
                     MIEKTGNKELALIMNFERTGCHFQMILETKNEPGKRTFVFPFKKVLNAAKRLGQFDLT
                     EEEILESWWKPRYLNYAQAMGVEFIISDLDENGTVTVELVD"
BASE COUNT          892 a          544 c          608 g         1089 t
ORIGIN      
        1 tattggcatt caagaattca ggatcgattg tgtcaacacc ttggtctttg gcagcattga
       61 ccatatggtt aatggccata tccattgcaa aatctgggac ctgtgtcaat gcttcgcaag
      121 catcgtcagt aaatgtaata ttttcatccg cactatttgc tttctcttta attgtttctg
      181 catccatgat ttttcctttt ttagctaaag taagtattag tggttaattg tgtcaaattt
      241 ttcttcaaac gtgaagagta attgttaagg tgattgagtt taaccaatga ggtgcgtgag
      301 caaaccatca agtaattttg gttcaaacca ggtagatttc ggtggcatga tttgatgtgc
      361 ttcagctaca gagatgagag cgttcattgg agttggatac aaagagaaac caattgccat
      421 ttctccagaa tccactcttt cttggagtgc ttcaatgcct ctgatcccac ctacaaaatc
      481 aattctctga tcaattcttt cgtcctggat tcctaatatt ggttctagga ggtaatcgtg
      541 cagaagggag acatccagtg attttactgg atctattgct tttctatgtg tttgtttgag
      601 ctccaattta taccagttcc cttgaaaata cataccaaat tgattggact gggtgggttt
      661 aaaggtcgaa accattgttt caatatcaaa aaattgatca gtcgcttgta ataaagattt
      721 ttttgaatgg ccattggcat ctttagtgac tcgattgtag tctagaatgt tcatttcatc
      781 ttcagggaat gcgaccacga gaacataatt gtagttttct gtgccattgt ggtctttatt
      841 tgctgctttt ttgttttttc taacctttgc tgcagcagct gatcgatgat gaccatcggc
      901 tataaataag gcgtccatat cgtttaatag ggctagaatt gatttaagcg tattggcgtc
      961 agataaccgc caaatcttat gtctggatcc atcgtttgct tctacgtcat aaaggggaag
     1021 tgtatgaata gtgatttctg agagtatgtt ttttaagtca tcattctttt tgtgtgccag
     1081 caatacagga cctgtttgag catttagggt ggcaatatta ttgaatcgat ccttttcttt
     1141 ttttggtgtg gtgtgttcat gtttcttgat taggttatgt tcgtatgcat ccaatgatgc
     1201 aatgcaacca ataccggttt gtttatgggt gtctagtgag atttcataaa catagatcga
     1261 atctatgtct tcttctacta gaatttcttc atcgataagg tttttgaaat ttattaaacc
     1321 ctgttggtag acctcaggat tgtcatatga ggtactaacg ggtaaatcga tttcaggcct
     1381 tgaaatatgg aggaagctca gtggtttatt tgctgctaat atcctcgctt catccgagct
     1441 tataacatca tagggtggag caatgacctc taatactttt tcaggtattg gtcgtagtgc
     1501 tttaaatgct ttaactagat tattttttcc tgtctcagtc atttatttaa tttttgccaa
     1561 aaaagaatca gtacttattt actataggtt aaaattatta tgtacaaagg tggattatat
     1621 tcgataattg taagtcactt ttaaaaaatc aaggagagta atttttgtga taccggctca
     1681 tattcaaaaa gcagtcagca gcaagaatag acctgcaagc gatcaggaaa gagattcatt
     1741 aagaaaacca ggcgagattc taaatttctt tgatgtaaag gaaggggaca agattggaga
     1801 actcaatgct ggaagaggct atttttcatc aattgcagca aatacagtgg gagaacatgg
     1861 aattgttatt gctcatacat cgccaatgag cgttaaacgc tggaaaggca atccaattga
     1921 aaaaagactt gaagaattcc cacaatccaa cctgattcca gttgttggag aaatggagtc
     1981 acctaatttt ccaggtcagc tagataagat atttaatata atgacctatc atgactcagt
     2041 ctggacagga accaataggg atcgcatgaa tacaagtgtt tttaatacct tgagagatac
     2101 tggcatttat ggaatattgg atcatcatgc caaacttggt cgaggtaaag atgattgcca
     2161 taaaattcac cgtattgaaa aagaatttgt tattgatgag attctgaggg ctgggtttat
     2221 tttggaaaaa gaatccaatc tttttaagaa ccctgaagat gatttgactt caatggtttt
     2281 tgaaaagggg atcagggatc aaaccagtcg attcctattg tgtttcaaga agtcttagtg
     2341 ctagtcaatc aacagattat tgattcaatc taccaattca actgtgacag tgccgttttc
     2401 atccagatca gagattataa attccactcc catggcctga gcataattaa gataacgggg
     2461 tttccaccag ctctctagaa tctcttcttc agtgagatcg aattgaccca atcttttagc
     2521 tgcattcaat acttttttaa aaggaaaaac aaatgtccgt ttccccggtt cattctttgt
     2581 ttccaatatc atctggaaat gacaacctgt tcgttcgaaa ttcataatca atgccagttc
     2641 tttattgcca gttttttcta tcatagcttt ggatcgttca agtaaaggct tcatggtttc
     2701 aagatcgtgt gctaccagct cttctagaag tcctttattt tttgcaaact gaatagtatt
     2761 caggtgtgcc ttcagcaagg catcatttgt atcatgaggg tagggcattt gcatttttat
     2821 tgttttcacc agtacctgca tcgctgcagc taaatctgct tcccttcctt ccattcctgt
     2881 tggtggagct aaattttctg gtttcatagc acttacagga ggtccatcct ttttgttatc
     2941 tgtgttatat tgttttctat tttcttccat aattcaaatc ctaagcacct tatcaatgtt
     3001 aaaaaaatct gtgtaattat agcctatgtc tgatgaatca gacaggacgt aaaatcataa
     3061 attcgatata atatttgatc tcccagtgta aagattttta ttaatattat tgcaatagtc
     3121 aattcttcta ata
//
LOCUS       DASCTH010000075         4572 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000106155, whole genome shotgun sequence.
ACCESSION   DASCTH010000075 DASCTH010000000
VERSION     DASCTH010000075.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000106155"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="QGH35_01555"
     CDS             <1..339
                     /locus_tag="QGH35_01555"
                     /inference="COORDINATES: protein motif:HMM:NF020468.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HJL81253.1"
                     /translation="GPKTVWLHNNTNYPPGLSAPRMQRMTMFAPLDQFLDQNVKSLPS
                     LFSATVLMPWLSWMDMDEVEGHTLWHASGVKLDSIDQLPKEYYNRMMSEHPDLSSFDL
                     ENDSGPVIYE"
     gene            311..784
                     /locus_tag="QGH35_01560"
     CDS             311..784
                     /locus_tag="QGH35_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HJL81254.1"
                     /translation="MTVVRSYTNKLIVFLFIFCVGCAQDDNDRATQDEPVINSPSQDI
                     QMIRAENINGNLDRGRKLYLQCRACHSLKKNESHKLGPNLFGIFDQQAGTQEGFTYSL
                     PLIESNLIWDQDNLDRWLAAPYELVPGNKMVFSGMRQEKDREDLLAYLYVETFEQ"
     gene            844..1521
                     /locus_tag="QGH35_01565"
     CDS             844..1521
                     /locus_tag="QGH35_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HJL81255.1"
                     /translation="MNNDSFLSLLAESPFSGLQEHMEVDNKASEALKSFIKSAVESDW
                     KTAKEHRETIVKLEHQADEIKNNIRNHLPKSLFMSVSRQDILDLVFTMDGIPNAAKDI
                     SGVMIGRKMVIPEHMSKRFIDCTNSAITATHQACDAIKKVNEMQRSGFGGKNSNQLHH
                     LVAEIEQLEQENDELEISLRNELFKIEQDLPPVDVIFLYDIINKIGILADISQTLGHI
                     LIRLVAK"
     gene            1537..2805
                     /locus_tag="QGH35_01570"
     CDS             1537..2805
                     /locus_tag="QGH35_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008044323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HJL81256.1"
                     /translation="MFEIIAEHGQTLLIIACIVGFFMAWGIGANDVANAMGTSVGARA
                     LTLAQAVLVACIFEFAGAYLAGGEVTSTIRKSIIDSAIIGQTPELLVFGMIASLLAAG
                     IWLLVASYFGWPVSTTHSIVGAIVGFASVGIGFDVVMWPKVASIASSWIVSPVLAGTI
                     SFLLFTSVKRLILQKQDPFASAKRYVPFYMFLAGFLMSMVTFLKGLKHVGLSFTFSQS
                     IAWSLVFALVVTLIGTLLLQRIDNTIREKNGAMFDGVERVFAVLMVFTACSMAFAHGS
                     NDVANAIGPLAAVVSVVQSGGVVAAQSTIPSWVLLIGATGIVIGLAMLGLRVMMTVGR
                     AITELTPSRGFAAELAAAGTVVIASGTGLPISTTHTLVGAVIGVGLAKGIGAINLSVV
                     GKIILSWIVTLPVGAGLSILFFYALKGFFA"
     gene            2865..3926
                     /locus_tag="QGH35_01575"
     CDS             2865..3926
                     /locus_tag="QGH35_01575"
                     /inference="COORDINATES: protein motif:HMM:NF028240.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HJL81257.1"
                     /translation="MIKFIFTLFVLLTWSFLVTVNANENPIEFKGDFRYRYEFIDDAS
                     KDSSSRKNRIRGRFAAIARVNEKLKINLRFATDEENPTGANQDLGHDFSLKDIGLDQY
                     YFAYSISENTSIWGGKIENPYFKASKSELIFDGDYNPEGLVLQFKKGMVFGSLGAFSM
                     DEHESVDDISIAGFQVGISNKTSTGAKYKIALARYSFDDIRGRPASEVTWNGKIFGNP
                     TDASQNYLNDYILNNLTAEISTNMGSRKTPTVFFVDWVTNNDASSDDKGYQIGFKVSL
                     TDAWKITYLYKDVEKNAIFGALTNADFGGGGVGHKGHVVNLGYAFSKKFSFEFTWFEN
                     EKNRTTDYQRMFLDLKYKY"
     gene            4097..>4572
                     /locus_tag="QGH35_01580"
     CDS             4097..>4572
                     /locus_tag="QGH35_01580"
                     /inference="COORDINATES: protein motif:HMM:NF017591.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HJL81258.1"
                     /translation="MAKTTDRSITIFDRRKFIQFFGLSSALLTSSYGLTNSFSGNAKE
                     FLKRITKELSWKPITLAIPQISDGLIPDEQVDFYAQHETQDDLILPEGFTYNVIASWG
                     DPVGDSRYGYNNDHIGVVETSNHQAFLVVNHETIDYDNFETYLETFPMVMGYSLPD"
BASE COUNT         1409 a          773 c          971 g         1419 t
ORIGIN      
        1 ggtccaaaaa cagtttggct gcacaataat accaattacc ctccaggatt atcagctcca
       61 agaatgcaga gaatgacgat gtttgcacca ctcgatcaat ttctggatca aaatgttaaa
      121 tctcttccca gtctattcag tgcaactgtg ctgatgccat ggctgtcttg gatggatatg
      181 gatgaggttg aaggccatac tctttggcat gcctcaggtg taaaacttga ttcaatcgat
      241 cagcttccta aagaatatta caataggatg atgagtgaac acccagattt gtcatctttt
      301 gatcttgaga atgacagtgg tccggtcata tacgaataaa ctaatagtat ttctttttat
      361 tttctgtgtt ggttgcgctc aagacgacaa cgacagagca acgcaagacg aaccagtaat
      421 caatagtccc tctcaagata tacaaatgat cagggcggag aatattaatg gtaatctgga
      481 cagaggcagg aaactttatc tccaatgcag agcttgtcat tccttaaaaa agaatgaaag
      541 tcataaacta ggacccaatc tttttggaat ctttgatcaa caagcaggaa cacaagaagg
      601 ttttacatat tctctacctt taattgaatc aaacctaatc tgggatcaag ataatctgga
      661 tcgttggctg gcagcacctt acgaactagt gccaggaaac aaaatggttt tttctgggat
      721 gagacaagaa aaagatcgag aagatttatt ggcctatctt tatgttgaaa cttttgaaca
      781 ataaatattg aactgtaata attaatcagt aatatcagcc aataacaaag tgaggtaatc
      841 tatatgaata atgactcttt cctgagtttg cttgctgagt cgcctttttc cgggcttcag
      901 gaacacatgg aggttgataa caaagccagc gaggcactaa aaagttttat caaatctgca
      961 gttgaatctg attggaaaac tgcaaaagaa cacagagaaa ctattgtaaa acttgaacat
     1021 caagcagatg aaataaagaa taatattcgc aaccatcttc caaaatcatt atttatgagc
     1081 gtctccaggc aagatattct tgatctggtt tttacaatgg atggtatccc caatgccgcc
     1141 aaagatattt ctggggtaat gattggcagg aagatggtga ttccagaaca catgtctaaa
     1201 cgattcatcg attgtactaa ttctgcaata acggctactc atcaagcttg tgatgcaata
     1261 aagaaagtta atgaaatgca aagaagtgga ttcggtggaa aaaattcgaa tcaattacat
     1321 catctggttg ctgagataga acaacttgaa caagagaatg atgagttgga aatatcatta
     1381 agaaacgaat tatttaaaat tgaacaagat ttgccacctg ttgatgtcat ttttctctat
     1441 gacatcataa ataaaatagg gatccttgca gacatatcac agacattggg ccatatttta
     1501 attcgattag tagctaaata ataataagga taaatgatgt ttgaaatcat agcagagcat
     1561 ggacaaacct tactgattat tgcttgtatt gtcggtttct tcatggcttg gggtattggc
     1621 gcaaatgatg tagccaatgc catgggtact tctgtcggtg caagagcatt gactttagct
     1681 caagctgtgc tggttgcatg tatttttgaa ttcgcagggg cttatctggc aggaggagaa
     1741 gttacatcca ctattagaaa aagcattatt gattcggcaa taataggtca aactccagaa
     1801 ttgctagttt ttggcatgat tgcttccttg ctggctgcgg gcatttggtt actggttgct
     1861 tcatattttg gttggcctgt ttcaacaaca cattccattg ttggtgcaat tgtgggtttt
     1921 gcttcagttg gtattggctt tgatgttgtt atgtggccaa aagttgcttc catagcatcc
     1981 agttggattg tgtctcccgt tttggcagga acaatatcgt ttttgttgtt taccagtgtt
     2041 aaaagattaa ttctacagaa gcaagatcca tttgcgagcg ccaagcgata tgttcctttt
     2101 tatatgtttt tagcggggtt tttaatgtcg atggtcactt tcttaaaagg attgaagcat
     2161 gtgggtttaa gttttacttt ctcacaaagt attgcttggt ctttagtttt tgccttagtg
     2221 gtcactctta ttggcacact tctattacag agaatagaca atacaataag ggaaaaaaat
     2281 ggagctatgt ttgatggggt agagcgagta tttgcagtgt tgatggtttt tacagcatgt
     2341 tcgatggcct ttgcccatgg atcaaacgat gtagctaatg ccattggtcc tttggccgct
     2401 gtggtttctg tagtacaaag tggaggagta gtcgccgctc aatcaacaat accatcatgg
     2461 gtattattga ttggtgctac tggtatcgtc attggtcttg ccatgcttgg tttaagagtc
     2521 atgatgactg taggtagagc catcactgag ctgaccccaa gcagaggttt tgctgcagag
     2581 ttagcagctg ctggaaccgt tgtcattgct tcgggtactg gtttacccat atcgacaacc
     2641 cacacactgg tgggtgctgt aattggcgtt ggtcttgcca agggtatagg agcaatcaat
     2701 ctaagtgttg tgggtaaaat tattttgtct tggattgtca ctctaccagt aggagcaggc
     2761 ttatcaattt tattctttta tgctctaaaa ggcttctttg catagtttaa aaattgtgga
     2821 gtctcatcaa taaataaaag attagatcaa caaaggaaga gtgaatgatt aaatttatat
     2881 ttaccctttt cgtcttgttg acgtggtctt ttttagtaac agtcaatgca aatgagaatc
     2941 ccattgaatt caagggtgat tttcgttatc gctacgagtt tatcgatgat gcaagcaagg
     3001 actcatccag cagaaaaaac aggatccgag gtcgctttgc tgcaattgct agagtcaatg
     3061 aaaaactaaa gattaatttg agatttgcaa cggacgagga gaatcctacc ggagcaaatc
     3121 aagatttagg tcatgatttc tcacttaagg acattggttt ggaccaatat tattttgctt
     3181 actctatttc cgaaaatact agtatctggg gaggtaaaat agaaaatccc tattttaaag
     3241 ccagcaagag cgaattgatt tttgatggag attataatcc agagggtttg gttcttcagt
     3301 ttaaaaaggg aatggtattt ggctcactag gagctttttc gatggatgaa catgaatctg
     3361 ttgatgatat ttccatagca ggttttcaag ttgggatttc caataaaaca tcaacaggag
     3421 ctaaatataa aattgcattg gcacgataca gtttcgatga tattcgtggc aggcctgcat
     3481 cagaagtaac atggaatggg aagatttttg gtaacccaac tgatgcaagt caaaattatc
     3541 ttaatgatta tatattgaac aatcttactg ctgagattag caccaacatg ggctctcgaa
     3601 aaactccaac ggtttttttt gttgattggg tgaccaacaa tgacgcaagc agtgatgata
     3661 aaggctatca aattggcttt aaagtctcac tcactgatgc atggaaaata acttatcttt
     3721 ataaagatgt tgagaagaat gcaatttttg gtgctttaac taatgctgat tttggtggtg
     3781 gtggtgtagg acataaaggt catgttgtta acttaggtta tgcgttctca aagaaattta
     3841 gctttgagtt tacttggttc gaaaacgaaa aaaataggac aaccgactat cagagaatgt
     3901 ttctagatct taaatataaa tattaagaat aactgggtgg ctgttttcga aacacgatct
     3961 tgaaaccctt taggggaata gtctctgaaa attaacattc aaacaccaac gaatgtctta
     4021 tactaagcca ttctcaaatt ataattatct taaagatgga gagaattaaa attagtaata
     4081 atttgaaaac acttagatgg caaagactac cgatagatca ataacaattt ttgatcgaag
     4141 aaaatttatc caattttttg gactaagctc agcactatta acgtcaagtt atggtttgac
     4201 aaactctttt tctggcaatg cgaaagaatt ccttaagcga attactaaag aattatcttg
     4261 gaaacccata acgttggcaa ttcctcagat ctcagatggc ttgattccag atgaacaagt
     4321 tgatttctat gctcagcacg aaacacagga cgatttgatc ctaccagaag ggttcaccta
     4381 caatgtgatt gcatcctggg gtgatcctgt aggcgattcc cgttatggct ataataatga
     4441 ccacatcgga gtcgttgaga caagcaatca tcaagcgttc ttagtcgtca accatgagac
     4501 cattgattac gacaattttg agacctattt ggagactttc ccaatggtga tgggctatag
     4561 cttaccggat ga
//
LOCUS       DASCTH010000076         5327 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000109965, whole genome shotgun sequence.
ACCESSION   DASCTH010000076 DASCTH010000000
VERSION     DASCTH010000076.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000109965"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="QGH35_01585"
     CDS             complement(<1..388)
                     /locus_tag="QGH35_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81259.1"
                     /translation="MTEKQEHDYKAIDAVVNIWTEEALSHRPDWGDEFFVDKMNTQSA
                     LMAGLTLEQMIEKMDEAGIERAFLIAAHSGRVGLPGCYHMPYDVVARACEQYPDRFYG
                     LAGIDPFDGMNGVRAFEDAVTNMGFIG"
     regulatory      432..531
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            521..2635
                     /locus_tag="QGH35_01590"
     CDS             521..2635
                     /locus_tag="QGH35_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81260.1"
                     /translation="MHSKFRSILLFSLLTVLSPLTVMAAAAIEEIVVKASYRETTLEK
                     NDGSLMILSEEQLKAEPMKHLEQLSFLVPNLNFALSDGRPRYFQIRGIGEQSGYEGTP
                     NSSVGLIIDDIDFSGQGGISSSFDMEQIEVHRGPQGSRMGANALAGMIYMRSKQPTDV
                     FEGLSEFSLGSDGVRSVGVAFGGPLHDNSAIKYRVSIRNDESDGFRKNSYLNRDDTTG
                     KDELTVRLKLNHRPSADTELNVLIQKSDFEALSDSWTTDGSLNTLSDKPGFDSQDSDA
                     YGLRVNHRGNGFSLQSLTSATSADIIVSYDADWSNPVANAPYTYDFFSETLRSRRAFN
                     QEFRVLSNPLDYSIDNTFAWVVGAYYLNLNERNDITDLGTYINPFSSWPPYIKNVTGT
                     RNYSSENSALFGTFDYLLSETLTVSFGLRWEDWEAHYDDTFGEHFNPSDQMLGGKLSL
                     MKEWSDNTNLYASIGRGYKAGGFNLGTGFSNQLYTDTLIYDPEYLWNYELGINHQFAN
                     SNTHLDAVIFYSDRKDQQVMASTQMDSNDPNTFTFLTQNAASGENYGLELSLQSNPTD
                     SLQVFASLGLLQTEVNNFDSNSQQNGRGQAHAPERSFAVGMRWFPNDSIYFGFDLNGK
                     SDFYYSDSHDNQSSSYTLANLMIGYQQDHWNYEIWVRNLFDEYYSLRGFYFGNVPPNF
                     PSTLFERQGDPRHLGILIRYEF"
     gene            2625..3218
                     /gene="pnuC"
                     /locus_tag="QGH35_01595"
     CDS             2625..3218
                     /gene="pnuC"
                     /locus_tag="QGH35_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008490997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HJL81261.1"
                     /translation="MSFDPLMDNLAEIIGVIFAIGYLLLAVRQIIWCWLAWILSSLLY
                     LYVMFNAGLYMEAALQIFYVAMGLYGWMQWSKGGTEEHLVVRRWGLGNHLFAVSVILI
                     LTLLSGEVLSNYTTAAMPFMDALTTWGAIVTTYMVAKKLIENWIYWFVIDSISIYLFM
                     SRELYFTAVLFFVYLFIIIIGYRSWKQMELVRGESSH"
     gene            3202..4032
                     /locus_tag="QGH35_01600"
     CDS             3202..4032
                     /locus_tag="QGH35_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013775.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HJL81262.1"
                     /translation="MNLHIKTIEAIPHSITLGECLKDGPVTQVFTGVFEGIKAVIRLD
                     LPVSQTLGLNRYSEIELLKFLEGSTFTPKILFAAPENGVLIWQYIPGVTLDDTTTKPR
                     EITSLMGTLLADLHQLNAPASQPVFSSFINCYRDLLSAEMNHSMIHKGFSLFDSIAKN
                     QLPMVLSHNDINPGNLLIGEKLFIMDWEYASLNHPYFDLASSIENLALNKQQIKEFIQ
                     SYEINGFAVDYEELALWREFSLYLSFFWLMIIKKYATISEKEKAWMVNLENRLSQVKG
                     "
     gene            4037..>5327
                     /locus_tag="QGH35_01605"
     CDS             4037..>5327
                     /locus_tag="QGH35_01605"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HJL81263.1"
                     /translation="MAKVKTFDFDTLSNHAGQRPDKETGSRAVPIYQTASYVFRDSDA
                     GAAIFNIERGGHVYSRISNPTTAVLEERISALEGGVGAVATSSGQAAVFNTIATLVDQ
                     GGHIVSSYALYGGTHNLMEYTLPRFGITTTFVDPNDIEGFKKAIRPETRLIFGETVAN
                     PRTEVLNIPKVAKIAYDHHIPLVIDSTTTTPYLMKPFALGADIVVHSATKFLSGHGTS
                     MAGLIVDGGTFDWESTDKFPQMTEKYAGFHNLSFTEEFGPAAFISRVRNEASRDFGAC
                     LAPHSAFLVLNGTETLSVRMDKHISNTRQVVEYLDQHDMVDWVSHPDLKNHPNHELAK
                     ELLPKGSTAVFSFGIKGGREIGQVFIDRLDLFSNLANIGDAKSLVIHPASTTHQRMSA
                     KDLEEAGISEGLIRLSVGIESVEDLIEDLEKGFRAASK"
BASE COUNT         1493 a         1054 c         1174 g         1606 t
ORIGIN      
        1 cgccaataaa tcccatattt gtcaccgcgt cttcaaaagc tctgactcca ttcatgccat
       61 caaacggatc aatgcctgcc aatccataaa aacgatcagg gtattgttca caagccctag
      121 ccaccacgtc atagggcatg tgatagcaac ccggcagtcc aactcgacct gaatgagcgg
      181 cgattaaaaa ggctctttca atcccggcct cgtccatctt ttctatcatt tgttcaagag
      241 tcagtccggc catcagtgca ctttgggtat tcatcttgtc aacgaagaat tcatcgcccc
      301 aatccggtcg gtgagaaagg gcttcttcgg tccaaatatt aaccacagca tcaatggctt
      361 tataatcgtg ttcttgtttc tctgtcataa gggcaacata gcaaaactta agtcgatata
      421 ctatcatagc cttagattgc aaggggtggc tagtgagtct gagatctata aaggaaccct
      481 ttgaacctga accatataat gatggcggag gaatagcata ttgcattcaa aatttcgatc
      541 aatcctactg tttagcctgc tcacggtcct ctcaccgttg acagtcatgg ctgcggctgc
      601 cattgaagaa attgtggtca aggccagtta tcgagaaacc actctagaaa aaaatgacgg
      661 gagtctgatg atccttagtg aggagcaatt aaaggcagaa cccatgaaac atttagaaca
      721 actctcattt ttagttccta atttaaattt tgctcttagt gacggacgcc caagatattt
      781 tcaaatcaga ggcattggtg aacaatcagg ttacgagggg actcctaatt catccgttgg
      841 gttaattatt gatgacattg atttttcagg tcaaggtgga atctctagca gttttgatat
      901 ggaacagatt gaagtgcatc gaggacctca aggttcccgt atgggtgcca atgcactggc
      961 tggaatgatc tacatgagat caaaacaacc cacagatgtt tttgaaggat tgtccgagtt
     1021 cagtttgggt tccgatgggg tgagatcagt tggtgtggcg tttggaggac cgcttcatga
     1081 taattcggca atcaaatatc gagtgtcaat tagaaacgat gagagcgatg gatttcgtaa
     1141 aaattcttat ctcaatcgcg acgatactac cggcaaagat gagctgacag ttcgactaaa
     1201 attaaaccat cgaccgagcg cagatacgga gttgaatgtt ctgattcaaa aatctgattt
     1261 tgaagctctg tccgattcat ggacaaccga tggcagtctt aacactctct ctgacaaacc
     1321 aggctttgat tcacaagatt ctgatgcgta tggccttcga gttaatcatc gagggaatgg
     1381 tttttccttg caaagtctca ccagtgcaac gagtgccgat atcattgtca gttatgatgc
     1441 cgattggagt aatccggttg ccaatgctcc ttatacctat gacttcttct cagagactct
     1501 tcgctcacga cgagcattca atcaagagtt cagagtgctc tctaatcctc ttgattattc
     1561 gattgataac acctttgcct gggtggttgg tgcgtattat ctgaacttga atgaacgcaa
     1621 tgatatcact gatttgggaa cgtatattaa cccgttcagc tcctggcccc cttacataaa
     1681 aaatgtgacc ggcacaagaa actatagttc tgagaatagt gctttgtttg gaacatttga
     1741 ttatctgctt tcagaaacac tgactgttag tttcggtttg agatgggagg attgggaggc
     1801 ccattatgac gatacttttg gggaacactt caaccccagt gatcagatgc ttggaggtaa
     1861 gttatctcta atgaaagagt ggagcgataa caccaatctc tatgccagca ttggcagagg
     1921 atacaaagct ggaggcttca atcttggcac tggtttttct aatcaactct atacggatac
     1981 attgatttat gatccagagt atttatggaa ctatgaactt ggaattaatc accaatttgc
     2041 caactccaat acccatcttg acgcagtgat tttttattct gatcgcaaag atcagcaggt
     2101 catggcgtcc acacaaatgg actcaaacga tccgaatact tttaccttcc tgacacaaaa
     2161 tgcagccagt ggtgaaaatt atggactcga gctgtcgcta caatcgaatc caacggattc
     2221 gctccaagtt tttgcttctc tgggtttatt acaaaccgag gtcaataact ttgattcaaa
     2281 tagccagcag aatgggagag ggcaagcaca tgcacctgaa agatcgtttg ctgtcggcat
     2341 gaggtggttt ccgaacgatt caatctattt tgggtttgat ttaaacggta agagtgattt
     2401 ttattactcg gattcacatg ataatcaatc atcctcatac acactggcca atcttatgat
     2461 tggttatcag caagatcatt ggaattatga aatttgggtt agaaatctct ttgatgaata
     2521 ctattcattg agaggttttt attttggtaa tgtgccaccg aattttccta gtactctctt
     2581 tgagagacaa ggggatccaa gacacctagg tatactgatt cgttatgagt tttgatccat
     2641 tgatggataa cttggcagag attataggcg tgattttcgc cattggttat ttgctattgg
     2701 cagttcggca aatcatctgg tgttggctgg cctggatatt aagttcattg ttgtatctct
     2761 acgtcatgtt taatgccggt ctctatatgg aagctgcgct gcagattttc tatgtggcca
     2821 tggggctcta cggatggatg caatggagca aaggcggcac agaggagcat cttgtggttc
     2881 gtcgatgggg tttgggtaat catttatttg cggtatccgt tattttaatc ctcaccctat
     2941 tatccggtga ggtgttgtcg aattacacca cagctgccat gccattcatg gatgcgttga
     3001 ccacttgggg agccatagta accacatata tggtggcgaa gaaattaatt gagaattgga
     3061 tctattggtt tgttattgat tcgatctcga tttatctgtt tatgtccaga gaactgtatt
     3121 ttaccgccgt tctgtttttt gtgtatttgt tcattattat tatcggttat cggtcttgga
     3181 aacaaatgga gttggttcga ggtgaatctt cacattaaaa cgatagaagc cattccacat
     3241 tctattactt tgggagaatg cttaaaggac ggcccggtta cccaagtgtt cactggcgtt
     3301 tttgaaggaa taaaagcggt tattcgtctt gatttacctg tttcacaaac actgggcttg
     3361 aatcgttata gtgaaattga attattaaaa ttcttggaag ggtcgacttt tacaccgaag
     3421 atactttttg ctgctcctga gaatggggta ttgatttggc aatacattcc cggggtaaca
     3481 cttgacgata caactactaa accacgagag atcacaagtt taatgggaac tttgttggca
     3541 gatcttcatc agcttaatgc accagccagt cagcctgttt tttcttcttt tattaattgt
     3601 tatcgtgatt tactgtctgc tgaaatgaat cattcaatga tccacaaggg cttttcatta
     3661 tttgactcta ttgctaagaa tcaattgcca atggtcttgt cacacaacga tattaacccc
     3721 ggaaatttat tgatcggcga gaaacttttt attatggatt gggagtatgc aagcttgaat
     3781 catccttatt ttgacttagc aagcagcata gaaaatcttg cattaaacaa gcagcaaatc
     3841 aaagaattta tacaatccta tgagatcaat gggtttgctg tggattatga agaactggct
     3901 ttatggagag agtttagtct ttatttaagc ttcttttggc ttatgattat taagaaatac
     3961 gctacaatat ctgaaaaaga aaaggcttgg atggtcaatt tggaaaatcg attatcacaa
     4021 gtaaaaggtt aaatttatgg caaaagtaaa aacattcgat tttgacacgc tttcaaatca
     4081 tgccggtcag cgacctgata aggagaccgg gtcgagagct gttccaattt atcaaacagc
     4141 ctcctatgtt tttagagact cagatgcggg tgctgcaatt ttcaatatag agcgaggtgg
     4201 ccatgtttat tcaagaatct ctaatcccac caccgctgtc ttagaagagc gaatttctgc
     4261 actggaaggc ggagttggtg ccgttgccac ctctagcgga caagctgctg tgtttaacac
     4321 catagcaact ctagtggatc aaggcggaca cattgtctct tcatatgctc tttatggggg
     4381 cacccataat ttgatggaat ataccttgcc acggtttgga atcaccacca cttttgtgga
     4441 tccaaatgat atagaaggct ttaagaaagc cattagacct gagaccagac taatttttgg
     4501 agaaactgta gccaatccaa gaactgaagt attaaatatt cctaaagttg ctaaaattgc
     4561 atatgaccac cacatcccat tggttatcga ttcaaccacc accacccctt atctgatgaa
     4621 accctttgca ttgggagctg acattgtggt gcattcagcc acaaagtttt tgagtggtca
     4681 cgggacttcc atggccggtt tgattgtcga tggcggtacg tttgattggg aatcaacaga
     4741 taagtttcca caaatgacag aaaaatatgc aggctttcat aacttatctt ttactgaaga
     4801 atttgggcca gcagctttta tttccagagt cagaaatgaa gcatcgcgtg attttggcgc
     4861 ttgtcttgct ccacattctg cctttttggt gcttaacggt actgagacat tatcggtgag
     4921 gatggataaa cacattagca atacccgaca ggtggttgag tatctggatc aacatgacat
     4981 ggttgactgg gtcagtcatc ctgatcttaa aaatcatcct aaccatgagt tagcaaaaga
     5041 actgttacca aaaggctcga ccgcggtttt cagtttcggt attaaaggcg gcagagagat
     5101 cggtcaagtg tttatagatc gattggattt attctccaat ttagcaaaca taggtgacgc
     5161 aaaatcttta gttatccatc cagcaagcac cacccatcag aggatgtcag ccaaagattt
     5221 ggaggaagcg ggcatctctg agggtttgat cagactgtct gttggcattg agtctgttga
     5281 agatctaatt gaagatctag agaaaggctt tagagcagcg tcgaaag
//
LOCUS       DASCTH010000077         4287 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000110085, whole genome shotgun sequence.
ACCESSION   DASCTH010000077 DASCTH010000000
VERSION     DASCTH010000077.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000110085"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(148..2586)
                     /locus_tag="QGH35_01610"
     CDS             complement(148..2586)
                     /locus_tag="QGH35_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018981858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HJL81264.1"
                     /translation="MVITSSIAWLLIFFIAIIVLCYKVVELRIATIILGIGLLICTIF
                     GNAAGVILVIFWVFFVLLLALNIPEIRRNYISTQVLKFYKSVLPKISQTEQEAIDAGS
                     VWWDGEIFSGNPDWDILRKNPKPKFSKEEQAFLDGPVNEICEMTNEWKASHIDHDLPQ
                     EVWDFVKAKGFFGMIIPKKYGGLEFSPLAVATVMSKVISRSGHLNSIIGVPNSLGPAE
                     LLLHYGTPEQRNDLLPKLASGEEIPCFALTGPHAGSDAAAMPDTGIICKGKFEGKEII
                     GMKLNFSKRYITLAPVATLVGLAFKLYDPNHLLGEKEDYGITCALIPSTTKDLEIGRR
                     HLPPGIPFMNGTIIGKDIFVPLDYIIGGTEQAGKGWRMLVECLSAGRAISLPSGAMGG
                     AKTGAWVTGAYTRAREQFNVPIAEFEGITEPLGRIAGNTYIITASLNTTAMAIGAGEK
                     PAVISAIMKYHTTELARKISRDAMDIHGGKAVMLGPKNYLASSHQASPVAITVEGANI
                     LTRSLIIFGQGAFRCHPFVLKEIAAIEEEDRDESLEQFDQHFFDHVAYSLSNASRSFI
                     LGLSKKFGNSPVVGETEGYYKQINRFSAAFAFLTDVSMLVLGGSLKRKETLSARLGDI
                     LSSLYLASTSLKYYEDMGDKEADLPLVHWACQTLIFEAQESFHQILLNFPNKIIGRLL
                     RCLIFPTGRKIKGPSIETTLKIGNLISRNTSTRNNLCRDIYLKDEPTNHLAIMQHALE
                     LYEVVYPIKTEVMTARKKGLIEGHTLEELVAAAENADIITKEQAKQLLAYDQVTLDII
                     NVDDFSEEELANIH"
     gene            complement(2730..3698)
                     /locus_tag="QGH35_01615"
     CDS             complement(2730..3698)
                     /locus_tag="QGH35_01615"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007641441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HJL81265.1"
                     /translation="MTTITITGAAGQIGYQLAFRIASGQLLGPDQKIKLKLLEIPPVL
                     DALKGVAMELDDCAFPCLESITATDDISIAFEHAEYAFLVGAKPRGPGMERSDLLIGN
                     AEIFSTQGKAINTYANHNIKVLIIGNPANTNALITMSNAPNIDPKSFTAMTRLDHNRA
                     LAQLARKTQTHVRDINKLIIWGNHSTTQYPDVHHTTINDQSALGRVNQNWIENIFIPT
                     VQNRGAEIIQARGLSSAASAASAAIDHVKDWTFGSANNDWVSMAVPSDGSYGVAKGIV
                     YSFPVICTNGSYEIVQGLDINEFSQECMNRTEMELLGERKAIEHLL"
     gene            complement(3729..4076)
                     /locus_tag="QGH35_01620"
     CDS             complement(3729..4076)
                     /locus_tag="QGH35_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81266.1"
                     /translation="MKSKVYSLLIIGTLSFITQTAIADCNFPNMNINFPNGSKASEQD
                     MIDTQKKVKTAQSGLIAYRECLDKELSSISPELENYPEIERISIDRYNSSVEAEQELA
                     ASWGESVKAFKAK"
BASE COUNT         1209 a          874 c          822 g         1382 t
ORIGIN      
        1 tttaatgtat tttataaacc ctcaggagct ggaggagggg atataggtct ggcgttgtca
       61 tcgtcaccag aaagtctgaa tgattttttg gatgaaatcg atcgattgaa ttggaatatt
      121 agttgcctgc cttcgacacc atgactatca atgaatattg gctagttctt cttcagagaa
      181 atcatcaacg ttaataatgt caagagttac ttggtcatat gccagcaatt gcttggcttg
      241 ttctttagtg atgatatctg cattttctgc agcagcgact aattcctcca aggtatgacc
      301 ttcaatcaac cctttcttgc gtgcagtcat gacctcggtt ttaatcggat agacaacctc
      361 atagagttct agtgcatgct gcatgatggc tagatgattg gtgggctcat ccttcaagta
      421 aatatctcgg cataaattat tcctggtgct tgtatttctt gaaataagat ttccaatttt
      481 aagcgtggtc tcaattgatg ggcctttaat ttttcgacca gttggaaaaa taagacaacg
      541 aagaagtctt ccaattatct tatttgggaa atttaataaa atctgatgaa aactttcttg
      601 agcctcaaaa atcaatgttt gacatgccca atgaaccaag ggaaggtctg cttctttatc
      661 tcccatgtct tcgtaatatt ttaaagaagt gctggcaagg tagagtgaac ttaaaatatc
      721 acccagtctt gctgacaagg tttcttttct ttttaaactg cctcccaaga ccagcattga
      781 gacatctgtc aaaaaagcaa atgctgcact aaagcgatta atttgcttgt agtaaccctc
      841 tgtttctccg accactgggc tatttccaaa ttttttggag aggcccaaaa taaatgatcg
      901 ggaggcattc gataacgaat aagcaacatg gtcaaagaag tgttgatcaa attgctctaa
      961 tgattcgtca cggtcctctt cttcaatcgc tgcaatttct ttcaatacaa aagggtggca
     1021 tcgaaatgca ccttgtccaa atataatcaa gctccgggtt aaaatatttg ctccctccac
     1081 ggtgatggct accggtgaag cttggtggga tgatgctaga taattttttg gtcccaacat
     1141 gacggctttt ccaccatgta tatccattgc gtccctgcta atttttcggg ctaattccgt
     1201 tgtatggtat ttcatgattg cggatatcac ggctggtttt tcaccagccc caattgccat
     1261 tgcggtggtg tttagcgatg ctgtaataat ataagtgttg ccggctatcc tgcctaatgg
     1321 ttctgtaatt ccctcaaatt ctgctatagg aacattaaat tgttctctgg ctcttgtgta
     1381 agcaccggtg acccatgccc cagttttggc accacccata gcacctgagg gtagtgagat
     1441 ggctctgcct gccgagagac attcaacgag catacgccag ccttttcctg cctgctcagt
     1501 gcctccaatg atataatcca agggcacaaa aatatctttc ccaatgatag ttccgttcat
     1561 gaaaggaata ccaggaggca ggtgtcttct gcctatttcc aagtctttgg ttgtagatgg
     1621 tattaaagcg caagtaatac catagtcttc cttttctcct aacaaatgat tgggatcata
     1681 tagtttgaag gctaacccca ctaaagtagc aacaggtgcc aatgtaatat agcgcttact
     1741 gaaattgagt ttcataccaa tgatctcttt gccttcaaat ttacctttgc aaataatgcc
     1801 ggtatctggc atcgctgctg catcagaacc agcatgagga ccggtgagtg cgaaacaggg
     1861 aatttcttca ccacttgcaa gttttggtaa caggtcgttt ctttgctcag gtgttccata
     1921 atgcagcaat aactctgcag ggcctaatga attggggaca ccaataatag agtttagatg
     1981 tccgctacgg gaaataactt tactcatcac ggtggctaca gccaatggtg aaaattccag
     2041 accgccatat ttttttggaa tgatcatgcc aaaaaaacct ttggctttaa caaaatccca
     2101 tacctcttga ggcaagtcat ggtctatgtg actagctttc cactcatttg tcatttcaca
     2161 aatctcattc acggggccat ccaagaaggc ttgctcttct tttgagaatt taggtttagg
     2221 gttttttctt aaaatatccc aatcaggatt gccgctgaat atttctccat cccaccaaac
     2281 acttcctgca tcgattgctt cttgctcggt ttgcgatatt ttgggtagca cagatttgta
     2341 aaacttaaga acttgtgtgc tgatatagtt tcttctgatc tctggtatgt tgagggcaag
     2401 caacagcaca aaaaataccc aaaaaataac taatataaca ccagcggcat tgccaaaaat
     2461 agtacaaata agaagcccaa taccaagtat tattgtcgca attctgagct ctacaacttt
     2521 gtagcacaga acaataatcg ctataaaaaa aatcaatagc caagcaattg aactggttat
     2581 aaccattaga tcagtataac tccattttta tggatacttg ctttgttttt aacaaaaaaa
     2641 gcatttgtat tttagtgtat tcacaattat caatttgatt aaatcaggat gataaattct
     2701 aatccagtga acaatgattc tctaagcttt cagagcaaat gttcaatggc ttttcgttct
     2761 ccaagcagtt ccatttcggt tctgttcata cattcttggc taaactcatt gatatcaagg
     2821 ccttggacaa tttcataaga accattagtg caaataacag gaaaagagta aacaatgcct
     2881 ttagcaactc cataactacc atcgctgggc acagccatac tgacccaatc attgttggca
     2941 gaaccaaagg tccaatcttt aacatgatcg atcgcagccg atgctgcgga tgccgcgctt
     3001 gataatcctc ttgcttgaat aatttctgca cctcgatttt gcaccgttgg gataaaaata
     3061 ttttcaatcc aattctggtt tactcgccca agtgcacttt gatcattgat cgttgtatga
     3121 tgaacatctg ggtattgggt ggttgaatga tttccccaaa taatgagttt attaatgtct
     3181 ctaacgtgtg tttgagtttt tcttgccaat tgcgccaatg cacgattatg atcgagtcgg
     3241 gtcatggcag taaatgattt gggatctata ttaggcgcat tggacatcgt aattaaggca
     3301 tttgtgttgg ctgggttgcc aatgattaga accttgatgt tgtgatttgc ataggtgttg
     3361 attgctttgc cttgtgttga aaaaatttca gcattcccaa taagcaaatc gctccgctcc
     3421 atacctggac ctcttggttt tgctcccacc aagaaggcat attcggcatg ttcaaatgca
     3481 attgagatat catcggttgc tgttatggac tccaaacatg gaaatgcgca atcatccagc
     3541 tccattgcaa caccttttaa tgcgtccaac actgggggta tttccaataa ttttagtttg
     3601 atcttttgat ccggtcccag caattgtccc gatgcaattc taaatgcaag ctggtaacca
     3661 atttgtccgg cagcaccggt aattgtaatt gtggtcattg atcctattga aagagttttt
     3721 tcgcttaatt atttcgcctt aaatgcttta actgactcac cccaagatgc cgcaagttct
     3781 tgctcagcct ccaccgaaga gttgtatcta tcaatgctta ttcgttcaat ttctgggtaa
     3841 ttttctaatt ctggtgatat tgaacttaat tctttatcta gacattctct ataagcgatc
     3901 agaccgcttt gagcagtctt gacttttttc tgagtgtcaa tcatatcttg ctcggatgct
     3961 tttgacccat tgggaaaatt gatattcatg ttgggaaaat tacagtcagc aatagctgtt
     4021 tgggtaataa aacttaacgt cccgattatt aaaagcgaat aaacttttga tttcatgcaa
     4081 acacctttta aattcttatt atattaaaag atcccaagta taaattagga ttgaatctag
     4141 atgaagtgat ttcagtcatt tctcattgtt ctcattgttc tcattgatca gaccactata
     4201 gtatagttgt tacaaatagt tacaaatgac tttccattat aaactaggct tggtttgtga
     4261 taatgattat taataaatgg ataaagt
//
LOCUS       DASCTH010000078        16380 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000110329, whole genome shotgun sequence.
ACCESSION   DASCTH010000078 DASCTH010000000
VERSION     DASCTH010000078.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 16380)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16380)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16380
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000110329"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="QGH35_01625"
     CDS             <1..989
                     /locus_tag="QGH35_01625"
                     /inference="COORDINATES: protein motif:HMM:NF017044.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HJL81267.1"
                     /translation="FDQNEIDRKKLIWLARIDQSLSDPNGIVGYVLPEIIFGQGHPYA
                     KPSGGSGTRESIQWMSRDDMVKYHQGSVNPSNATLLVVGDTTLDEIVKDLDDVLGNWK
                     DHASFENINLDYSIHNASKPGKIYLVDKPGAFQSLIVAGQLMPGVGSEDEFKITLMNA
                     VIGGDFTSRLNMNLREDKSWSYGVRSRVSANKGPSPMLVFAPVQSDQTIPSIQEIIKE
                     YEDYLGARSAEQAELESVVSKRSLGLIGRFETFGKLMSGVAEIITFNRDDDYLEMLAE
                     KYRSVELSDIHGAAQQYLTPKDWVWIIVGDLSLIQEGIEGLNLGEIEVLELD"
     gene            1053..1129
                     /locus_tag="QGH35_01630"
     tRNA            1053..1129
                     /locus_tag="QGH35_01630"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1088..1090,aa:Glu,seq:ttc)
     gene            1208..2182
                     /gene="dusA"
                     /locus_tag="QGH35_01635"
     CDS             1208..2182
                     /gene="dusA"
                     /locus_tag="QGH35_01635"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF013380.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HJL81268.1"
                     /translation="MMGHTDRHFRHLARMLAPDLRLYTPMIHAESIVYGPRKFIIEEN
                     QNQQNVAIQLAGNDPSIMSEAAAIVSKLNYDEININIGCPSSRVQNCKVGAALMNDPK
                     LVSQCVEAIMSKVDLPVSIKTRIGINNQDDYSFLYDFIAKTAQAGCDIFIIHARKAFL
                     NGLNPKQNRTVPPIDYEKVYRLKQDFQDLTIILNGEVNSIEQIEEHFSYVDGVMIGRK
                     AYKDPLFLTKIQNHFFRQTFDHGQQSSTQLECIKEMIDYAKRQINQKDTNPHLIVRHM
                     AALLKGCRGAKEWRQLVGFSQHKLENPNKALNQMLYFIENYEENLLEI"
     gene            complement(2145..3908)
                     /locus_tag="QGH35_01640"
     CDS             complement(2145..3908)
                     /locus_tag="QGH35_01640"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HJL81269.1"
                     /translation="MNTKNNQLQNWVNEVAALTNPSEVFWCDGSQEEYQNIITLMLDS
                     GDLSELNPDTFPNCYLHRSDPMDVARVEHLTFICTSEEDDAGPNNNWMEPNQAHRKLD
                     DLFANCMSGRKMYVIPYCMGPMDSKYSRCGVEVTDSPYVVANMFLMTRMGSKALKRIE
                     DENKFVKGMHSIGDLDPEKRFIMHFPEENIIRSIGSGYGGNALLGKKCHALRMASWQA
                     LKENWLAEHMLIVEIENPAGEKFYIAAAFPSACGKTNLAMLIPPEKFEGWKVRTIGDD
                     IAWLHMDNNGQLWAINPESGYFGVVPGTNEKTNRNAYQTIQSNAIYTNVGLTDNNEPW
                     WEGKLDGTPVKDWKGDDFSTDSHGKCAHPNSRFTVSAKNNPEYSNLADSPSGAPICAI
                     IFGGRRSELAPLVYEAKDWNHGVLVGASVGSETTAAAAGDTGVVRRDPMAMKPFCGYN
                     FADYWRHWLSFSKQSKNLPKIFHVNWFRQNAQGFLWPGFGENLRVLDWIIQRCQNKID
                     AQETPIGYVPLKKDIDVSGLNISDENLNELFHIDANQWLAEMKDIKEYFDEFGERLPN
                     ELLDEHSKVIKSLKDSLHSSQ"
     gene            4004..4672
                     /gene="tsaB"
                     /locus_tag="QGH35_01645"
     CDS             4004..4672
                     /gene="tsaB"
                     /locus_tag="QGH35_01645"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HJL81270.1"
                     /translation="MIKCIAIDTSTIRLTLAVLNDGEFFELNQENGMDHARDIYSNIN
                     KILNDARVELQELELIGFGLGPGRFSGLRVAAAATQAMSYIHKIPVCGISSLSVIAQQ
                     AAEIFSIEKIAVAIDANRNQIYFGCYRVSAEGLVVATYPDRLVDVDNFDFSERDYCGI
                     GSGWSQYNHLRDKGCVELIHQGEKVLPDAKIMIKLAIRDFKSGKTVESFEALPNYLMD
                     QVTN"
     gene            4683..5189
                     /locus_tag="QGH35_01650"
     CDS             4683..5189
                     /locus_tag="QGH35_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014706459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HJL81271.1"
                     /translation="MFLLTNRHLFFYGFLATVFLMLYVLYAQYVLGLEPCPLCILQRI
                     AVIFLGMIFLCMTLLPPKTTATKLTASIFFITFSLAGLGVAGRHVWLQHLPPDQIPGC
                     GGDLTYMISNFPLLEVFGMVFSGSGECAEINWSFLSLSMPAWALICFVLFALYGTWAI
                     YQTKYNGY"
     gene            complement(5195..7855)
                     /gene="glnD"
                     /locus_tag="QGH35_01655"
     CDS             complement(5195..7855)
                     /gene="glnD"
                     /locus_tag="QGH35_01655"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HJL81272.1"
                     /translation="MSIENPEKYKEFKTETLINTLNSHPFTVKNYKKFIKEKSDFLDT
                     SFSIHHISHLLLARSKLNDSIVISAAEIFFKQNQESINVIAVGGYGRNELHPYSDTDL
                     LLLIEKNEKKSFQDSLAKFLTFLWDIGIQVGHSTRTLQECLKEGSKDLTVATSLMEAR
                     YLYGSITTYQTLEKEITGNKLLWSNTQFLEGKKNEQLSRHSQFNNTAYNLEPNIKSGP
                     GGLRDIHSIFWVGKKILGIQQLQELNGLGIISNQQLSMLIHARDFLFSIRYALHALTQ
                     RKEDRLLFDYQRTLAKQFDYHDEQHLLGVEQFMQDYYKSAKTIGRLNEIILQIYTQKL
                     FQSKKSKIKTLNHSFQIRDNLVELRKGIQFKDNPSLLLEIFLLFQKHKDIQGIGSKTI
                     SMITENLHLIDDSFRENTENKKLFLDILKAPEGVTHELKRMNLYGVLGLYIPAFGKIE
                     GRMQYDLFHAFTIDEHTLLVVSNLRRLALKRFDHEYMEYSKKMQLIATPEILYIAGLF
                     HDIAKGRNEDHSKLGSLEAESFCLDHGMSKYESKLVSWLVKNHLILSLTAQRKDINDP
                     NVIRSFAIKVGDESRLDFLYLLTICDVRATNPNLWSTWKRQLFDELYLLTKKALREGL
                     ENPINKDELIAEKKNLVEAKLNGNQRKKGLTNLFSFLGESYFLKFKDQEIIHHSKIIM
                     EKKFLENPKIIINLQQDIIEGQTCVFIICQTQSNAFLTATGIFEQTNLTIHDAKVTEL
                     NNGYSIYNFYVQEMNGVAIEDKKKIQQIKSRIEKTLSVEDDSSPIVSRALSRRLRYFD
                     TKTSINFTEDHINNRTVMELISIDRPGLLFDIAKTLKTENIWIESAKIATIGERVEDI
                     FYLTTQNKVKLSKSNCEQLEKKLEKVINQS"
     gene            complement(7859..8629)
                     /gene="map"
                     /locus_tag="QGH35_01660"
     CDS             complement(7859..8629)
                     /gene="map"
                     /locus_tag="QGH35_01660"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008844171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HJL81273.1"
                     /translation="MSIDIKNKDEQEQMRISGKLASEVLDMIENHIKPGITTGELDQI
                     CHDYIVNDQNSIPANVGYKGYPATICTSVNHVVCHGIPSTKKLKHGDILNIDVAIIKN
                     SFYGDTSRMYLIGKPTQQGERISKIAYEALCQGIRQVKPGNQLGDIGHAIQLHTEKHN
                     YSVVKEYCGHGIGKTYHAEPQVLHYGIPHQGIEIKEGMTFTIEPMINAGEPSVKLMPD
                     QWTVVTRDHSLSAQWEHTILVTKDGFEVLTLSPNEKIV"
     gene            8810..9847
                     /gene="rpsB"
                     /locus_tag="QGH35_01665"
     CDS             8810..9847
                     /gene="rpsB"
                     /locus_tag="QGH35_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HJL81274.1"
                     /translation="MATITMRQMLEAGAHFGHKTRYWNPKMAQYIYGQRNGIHIINLE
                     TTLPLLEEACLFIENLAAESGTVLFVGTKPAVKNEILNAGKECEMPYVSNRWLGGALT
                     NNKTIAQSIKKLEDLEISLKDENLSKKEALTIRRASEKLNRSLGGIRSMNRLPDVIFI
                     IDVNYEKNAVQEAKALGIPIIGIVDTNSSPDNIDYIIPSNDDSRGTAKVITASIVESF
                     LKGKKSIPMLDDIDDDFVEVDAEGKISKDQIKRKKRIFVADKKITKKQASEDKVESDD
                     ASESDKIDDIGESENLYISTEEEQKNQDRDSDQSNDPSNSEPSVEEKTKPGSELPGSS
                     ESEDDEDKKDS"
     gene            9864..10745
                     /gene="tsf"
                     /locus_tag="QGH35_01670"
     CDS             9864..10745
                     /gene="tsf"
                     /locus_tag="QGH35_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HJL81275.1"
                     /translation="MKISAIEVKKLRALTNAGMMDCKKALVESQGDFQKAILVLKESG
                     QSKADSKSGRVAAHGIVSIEIEKAQRLALVLEINCETDFAAKDNQFVEFSNQISNLLV
                     NEKIENIDQLMDCSISKFERVEDFRKSVVAKLGENITIRRFVRIFYQGTLGSYVHGNK
                     IAALVDVDIDDKNLAKDLAMHVAASKPKYIVQTDIPKAIIDEESRILRSQAEQEGKPK
                     EIIEKIVTGKLNKHLNEITLLGQEYIKDPDLNISQLLKKSNATVNLFVRYEVGEGIEH
                     QDKNFADEVKEQVEASK"
     gene            10790..11503
                     /gene="pyrH"
                     /locus_tag="QGH35_01675"
     CDS             10790..11503
                     /gene="pyrH"
                     /locus_tag="QGH35_01675"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HJL81276.1"
                     /translation="MVNKQIKRILLKLSGEALLGKKEFGVDPDVLIYLAKEIKSILDL
                     GTQVGIVIGGGNIFRGEGLAQSGIDRVTGDQMGMLATIINALAIQDALERQGLVARVM
                     SALKVNEVCEDYIRRRAIRHLEKGRVTIFSAGTGNPYFTTDSAASLRAVEIEADLLIK
                     ATRVNGVYSEDPLKNQDANHFETISFDKFISKNLKVMDTTSIVMCKENNLPIRVYDIN
                     IDNALLDIVQGKSVGTLIM"
     gene            11519..12076
                     /gene="frr"
                     /locus_tag="QGH35_01680"
     CDS             11519..12076
                     /gene="frr"
                     /locus_tag="QGH35_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011588429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HJL81277.1"
                     /translation="MLEEINQETNERMHKSIDAFSVNLTKIRTGRATPDILNSIQIEY
                     YGNDTPIQQLSNITVEDSQTLTISPWEEKFIPEIERAILKANIGLNPVTTGNIIRIPL
                     PTLTEERRVELTKQVRSEEEQAKISIRNIRRDSIQDLKDFSNEKMISEDDLHKGQTEV
                     QVITDEMIKNIESLSEQKQKELMSF"
     gene            12080..12844
                     /gene="uppS"
                     /locus_tag="QGH35_01685"
     CDS             12080..12844
                     /gene="uppS"
                     /locus_tag="QGH35_01685"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HJL81278.1"
                     /translation="MKENPTEENVPKILNLPRHLGVIMDGNGRWAKLRRKRRFFGHEA
                     GVLKAKEIVENSVKLEIEALTLFALSSENLDRPAREVVSLMRLFERSIEKYTGLLIEN
                     NVRFKQRGNKEMLPKALVRKIEQIEAITSENSGLKLTLAIAYGSRQDIIRGILDIIDG
                     IDKGEINKDQIDESTLSSYLSFSDLPDLDLIIRTSGETRISNFMLWEMAYSEIHFTET
                     LWPNFENQEFFEILENYSKSERRFGAIEEEKELKKA"
     gene            12855..13682
                     /locus_tag="QGH35_01690"
     CDS             12855..13682
                     /locus_tag="QGH35_01690"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HJL81279.1"
                     /translation="MKNITKRICSAAVLILILIYLTQDSTANLAKIVVATLLSLIIVW
                     EYSRLFKSRIYQALFQILFVSFVLSAPYLTGILEKTSNYFFGLLVFAWVVILFRVLTY
                     KKKNIDDFLVFIFGYLTLLPFVGSLFTILIYPGILLYVVITVSIADSSAYLVGRKVGK
                     TALLPNISPGKTIEGFVGSLIITPIISSLIVYLFYGEVVIGFLFIGFFVTLISVIGDL
                     FFSLLKRNKNIKDSSNLIPGHGGFIDLLDGTIAVLPLLAFLFFKVSYLSMVLSVISF"
     gene            13775..15136
                     /gene="rseP"
                     /locus_tag="QGH35_01695"
     CDS             13775..15136
                     /gene="rseP"
                     /locus_tag="QGH35_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HJL81280.1"
                     /translation="MDFLFSIVAFIVLVGVLVAIHEYGHYLVARLCNVKVLRYSIGFG
                     KVLWSKNIGRDQTEYCLSAIPLGGYVQLLDERSEEVHESEVKRAFNNQSAVKRIFILS
                     AGPFANFLFSIVVYMTMFSIGVPGVVPIIGDVESDSIAWQNNLRSGDKIINVGGKDVK
                     TWQDSLISMIGDILDDEQIQIIAIDQLGNQKTVVLDVTGRTKELTSPNTLMPGLGFKP
                     FTPKIKPIIESVDLDGPADKAGLMSGDIILKVNDIDIKNWNHFREIIIANPNKEIDIE
                     IKRLDQVYRTTVLLDSRTDDVSQGFIGLRAGIDEEEIKKYQAIQQYNFPKNLTMALDQ
                     TNQMIVMTLNMFGKMVTGQISGKNLSGPLGIAKDAGTVAKRGLIATLSFMAIISISLG
                     ILNLFPLPILDGGQILFVLIEKIIRKPIPEKIQIIFQQIGVGALLFLMVFAVYNDLVR
                     IFS"
     gene            15155..>16380
                     /gene="bamA"
                     /locus_tag="QGH35_01700"
     CDS             15155..>16380
                     /gene="bamA"
                     /locus_tag="QGH35_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HJL81281.1"
                     /translation="MKIRLIIIALAFLCFYSVQAEESFVVKDIRIEGLQKISEGALLN
                     YLPINIGDILDDVKVKESIRSVYSSGFFKNIEFRQDESGILIISVLERPSIGNIVFDG
                     NKDIKTEDLEDSLNNIGFKVGRTYDPSVLDEIERSMTDQYFSFGKYSAQIETKVDDLP
                     GNTVNIQVNIKEGDRAQIRQINIVGNTIYNDEDLLDQLTLQMPDWLSFIQQDDRYSRE
                     DLQGDLETIESFYLDRGYANFRLDSAQVAISPDRTGIFITINITEGEVYQLSDVKLTG
                     EFVIPKEEIEQLVFAQPGQFFSQALLTQASELIKYRLGEDGYSFAEVEAIPELDRENK
                     EVKIVFYIQPKSRVYVRRVNFSDTTSINDEVLRRELRQLEGAYMSKSRLERSKVRLQR
                     LPYIEEVEFDTIPVAGT"
BASE COUNT         5172 a         2701 c         3209 g         5298 t
ORIGIN      
        1 tatttgatca gaatgaaatt gataggaaaa aattaatttg gctggcaaga atcgatcaat
       61 ctttgtctga tcctaatggc atagttggtt atgtgttgcc agagattatt ttcggtcaag
      121 gacaccctta tgctaaacct tcagggggca gtggcaccag ggaatccatt cagtggatga
      181 gcagagatga tatggtcaaa taccaccaag gttctgttaa tccaagcaat gcaactttat
      241 tggtggttgg cgataccaca cttgatgaga ttgtaaaaga tttggatgac gtactaggga
      301 attggaagga tcatgcgtcc tttgagaata tcaatcttga ttactccata cataatgctt
      361 ccaagcctgg taaaatttat ttagtggata aaccaggggc gtttcagtca ctcattgttg
      421 cgggtcaatt aatgcctggt gttggttccg aagatgaatt caaaattact ttaatgaatg
      481 ctgtcattgg aggtgatttt acatcaaggc tgaatatgaa cttacgcgaa gataaaagtt
      541 ggtcgtatgg ggtcagaagc agggtttctg caaataaagg accaagtcca atgctagttt
      601 ttgctccagt ccagagcgac cagaccattc catcaattca ggaaatcatt aaggagtatg
      661 aggactatct tggggcaaga tcagctgaac aagctgaact tgagagcgtt gttagtaagc
      721 gatctcttgg tttaataggt cgatttgaaa cattcggcaa gctcatgtct ggggtggctg
      781 aaatcataac cttcaaccgt gatgatgatt acctggaaat gcttgcagaa aaatatcgaa
      841 gtgttgaact gagcgatatt catggtgccg cacagcagta tctcactcct aaagattggg
      901 tttggattat agtcggtgat ttatctttga tccaagaagg aatagaaggt ttaaatcttg
      961 gagagattga agttttagaa ttggattaaa tctacttttt caatatactg cattcctgat
     1021 atatagcaga cgtaagaaaa ttattttttt gggtccccat cgtctagtct ggtcaggaca
     1081 ccaggttttc atcctggcaa caggggttcg aatccccttg gggacgccac cttagtacaa
     1141 ttaaaaagaa cctcacaaaa cctgaattat accaatgtta gaaccacatc aaatttcagt
     1201 tgcaccaatg atgggacata cagaccgaca ttttaggcat ctggccagaa tgctggcacc
     1261 agatttaaga ttatataccc caatgattca tgcggagtcg attgtttatg gaccgagaaa
     1321 atttatcatt gaggagaacc agaaccaaca aaatgtagct atacaactgg cagggaacga
     1381 tccttccatt atgtcagaag cagctgcgat agtatccaaa ctaaactatg atgagataaa
     1441 tataaatatt ggttgtccaa gttcacgggt gcagaattgc aaagtcggtg ctgctctaat
     1501 gaatgaccct aaactggttt cacaatgtgt tgaagctatt atgagtaagg tagatttacc
     1561 tgtttcaatc aaaaccagaa ttggtatcaa taatcaggat gactatagtt ttctttatga
     1621 ctttatagct aagacagcac aagctggttg tgatattttt ataattcacg ccagaaaagc
     1681 atttctgaat gggttaaacc ctaaacaaaa taggaccgtt ccaccgattg attatgaaaa
     1741 agtttaccga ttaaaacaag actttcagga tttaacaatt attctcaacg gagaagtcaa
     1801 ctcaattgaa caaattgagg aacatttttc gtatgttgat ggtgtgatga ttggtcgcaa
     1861 ggcttataaa gacccattat ttcttacaaa gattcagaat cactttttta ggcaaacctt
     1921 tgatcatggt caacagtctt caacacagtt ggaatgtatt aaagagatga ttgactatgc
     1981 aaaacgacaa ataaatcaaa aagacaccaa tcctcattta attgtgaggc acatggcagc
     2041 tttgctgaag gggtgtagag gcgctaaaga atggagacaa ctggttggtt tcagtcaaca
     2101 taaattagaa aatccaaaca aggctctaaa tcagatgctt tattttattg agaactatga
     2161 agagaatctt ttagagattt aatgacttta gaatgctcat cgagcagctc gttgggaagt
     2221 ctttctccga attcgtcaaa atattccttg atatccttca tttcagccaa ccattggttg
     2281 gcgtcaatat ggaataattc attcaagttt tcatcagaaa tatttaaacc agaaacatca
     2341 atgtcttttt ttagtggcac atagccgatg ggggtttctt gggcatcaat tttgttttgg
     2401 catctctgga taatccagtc taatactctt agattttctc caaatcctgg ccaaagaaat
     2461 ccttgagcat tttgcctaaa ccagttgacg tgaaatattt ttggcaaatt tttagattgt
     2521 tttgaaaaac taagccaatg ccgccaataa tccgcaaaat tatagccaca gaagggcttc
     2581 atagccattg ggtctcttct gactactcca gtatctccag ctgcagctgc agtagtttct
     2641 gagccaacac tcgcgccaac aagtacccca tggttccagt ctttggcttc ataaaccaac
     2701 ggcgccaact cacttctcct acctccaaaa atgatggcac aaatgggcgc tccggagggt
     2761 gaatctgcta aattcgagta ttctggatta ttttttgctg aaacagtaaa tcttgaatta
     2821 gggtgtgcac actttccatg actatcggtg gaaaaatcat cacctttcca atctttaact
     2881 ggagtgccat ctagttttcc ttcccaccag ggttcattat tatctgttag gcctacattg
     2941 gtataaatgg cattgctttg aatggtttga tatgcgtttc tattggtttt ttcattggtg
     3001 cccggaacca ctccaaaata gcctgattct ggatttattg cccataactg accgttatta
     3061 tccatatgca gccatgcaat atcatcgcca atagttctaa ctttccatcc ctcaaatttc
     3121 tcggggggga tgagcattgc gagatttgtt ttgccacagg ctgatggaaa agcagcagca
     3181 atataaaatt tttctcctgc cggattctct atttcaacaa taagcatgtg ttcggcgagc
     3241 caattttctt tcaaggcttg ccagctggcc attctaagag catggcattt tttacctaag
     3301 agggcattgc caccatatcc cgaacctatg cttcttatga tattttcttc tggaaaatgc
     3361 ataataaatc gtttttctgg atccaagtca ccaatcgagt gcatgccttt aacaaactta
     3421 ttctcatctt caattctctt caatgcttta ctgcccattc gagtcatcag gaacatattt
     3481 gcaactacgt aagggctgtc cgtaacttca accccacaac gagaatattt tgaatccata
     3541 ggtcccatac aataggggat tacatacatt tttcttccag acatacagtt tgcaaatagg
     3601 tcatctaatt tcctatgggc ttgattgggt tccatccagt tattatttgg acctgcatca
     3661 tcttcttcag aagtacatat aaaagttaag tgctcaactc tagctacgtc catgggatct
     3721 gatcggtgta gatagcagtt tgggaacgtg tctggattca gttccgagag atccccagaa
     3781 tcaagcatta aagtaataat attttgatac tcttcttggc ttccatcaca ccaaaagact
     3841 tcagatggat ttgtcagagc agcaacctca ttaacccaat tctgtaattg attatttttt
     3901 gtattcattt tatctcgctc taaaagagag tttattatat ctatgttttt tattggttca
     3961 ttaatttctt tactcatata ctgtttagca aggaaggtta tttatgatta agtgcattgc
     4021 aatcgataca tcgacaatca ggctgacact tgcggtcttg aatgatggag aattttttga
     4081 actcaaccaa gaaaacggta tggatcacgc tagggatatt tacagcaata tcaataagat
     4141 attgaatgat gcaagagtgg aattacaaga gttagagttg attggatttg gtttaggacc
     4201 cggtcgtttt agtgggttga gagtcgcagc agccgcaacc caagccatgt cttatatcca
     4261 taaaatacca gtttgtggta tttcaagcct ttcagtcata gcgcaacaag cagcagaaat
     4321 attttctatc gaaaagattg cagtagcgat agatgcgaat agaaatcaaa tttattttgg
     4381 atgttaccgt gtatcagctg agggtctggt cgttgcaaca tatccagatc gtttagttga
     4441 tgttgataat tttgatttct ctgaaagaga ttattgtggg atcggttcgg gttggtcaca
     4501 atataatcat ctgagagaca aaggttgtgt tgaattgatt catcagggag agaaagtatt
     4561 acctgatgca aaaattatga taaaactagc aatcagggat tttaaatcag gcaaaacggt
     4621 tgagtctttt gaagctcttc cgaattatct gatggaccaa gttacgaatt aaggaaagac
     4681 aagtgtttct attaacaaac agacatctat ttttttatgg attcttagca accgtttttc
     4741 ttatgcttta cgtgctgtat gctcaatatg tcttaggttt agaaccttgc cctttatgta
     4801 ttctacaaag aattgcagtc atttttttgg gcatgatttt tttatgtatg acactccttc
     4861 ctccaaagac cacagcgact aaattaacag cttcgatatt tttcataact ttcagtcttg
     4921 ccggactagg tgttgcagga aggcatgttt ggttgcaaca tttgccacca gatcagattc
     4981 ctggatgtgg aggtgatctt acctatatga tttctaattt cccattatta gaggtttttg
     5041 gaatggtttt tagtggttcg ggcgagtgcg cagaaatcaa ttggtctttt ttgtctttga
     5101 gcatgccggc ttgggcgttg atttgttttg ttttatttgc gttatacgga acgtgggcaa
     5161 tttatcaaac gaagtataat ggatactgat ttttctatga ttgattaatc actttttcaa
     5221 gttttttttc taattgttca caattagatt tactgagttt cactttattc tgagtagtta
     5281 aataaaagat atcttccact ctttcaccaa tcgttgcgat ttttgccgat tcaatccaaa
     5341 tattttctgt ttttagtgtt ttggcgatat caaacaacaa accaggtctg tctatagaga
     5401 ttaattccat cacagtccta ttattgatat ggtcttctgt aaagttgatt gatgtcttgg
     5461 tgtcaaaata tcttaatctt ctagacaatg cccgactgac tattggtgaa ctgtcatctt
     5521 caacactcaa tgttttttcg attcttgatt taatttgttg aatcttcttt ttgtcctcaa
     5581 ttgcaacacc attcatttct tggacataaa agttataaat agaatatccg ttattgagtt
     5641 ctgtaacttt tgcatcgtga atagttaaat ttgtttgttc aaaaataccc gttgctgtta
     5701 aaaacgcatt actctgtgtt tgacagatta tgaagacgca tgtttgtcct tcaataatat
     5761 cttgctgaag atttataatg atcttaggat tctctagaaa cttcttctcc atgatgattt
     5821 ttgaatgatg aattatttct tgatccttaa attttaaaaa gtaactttca cctaaaaatg
     5881 aaaatagatt tgtaagtccc tttttccttt gattaccgtt cagttttgct tcaacaagat
     5941 ttttcttttc agcaattaat tcatccttat tgatcggatt ttcaagacct tctcttaagg
     6001 ctttttttgt taacaagtat agttcatcaa aaagttgtct tttccaagta ctccataaat
     6061 taggattggt cgctcttacg tcacaaatgg ttaataagta taaaaagtcc aatcttgatt
     6121 catcccctac ctttattgca aaggatcgga ttacgtttgg gtcattgata tcttttcttt
     6181 gtgcggtgag agagagtatc aaatgatttt tgacaagcca tgaaacaagc tttgattcgt
     6241 atttgctcat tccatgatcc aggcaaaaag attctgcctc tagggaacct aatttcgagt
     6301 gatcttcatt acgacctttg gcgatgtcat gaaacaagcc agcgatgtat aaaatttccg
     6361 gagttgcaat caattgcatt tttttactgt attccatgta ttcatgatca aatcttttta
     6421 gtgctaaacg ccttaagtta ctgacaacaa gaagggtgtg ctcatcgatt gtaaatgcgt
     6481 gaaataagtc gtattgcatt ctcccttcaa ttttcccaaa cgcagggata tagagaccta
     6541 agacgccgta aagattcatt ctcttaagtt cgtgagtaac tccttctgga gcctttaata
     6601 tgtctaaaaa tagtttttta ttctctgtat tttccctaaa cgaatcatca ataagatgga
     6661 ggttttcagt aatcatgcta atagtttttg agcctattcc ttgaatgtct ttatgttttt
     6721 gaaataagag gaatatctct aataataatg atggattgtc tttaaattga atcccttttc
     6781 ttaattccac aagattgtct ctaatctgaa aggaatgatt cagagttttg atttttgatt
     6841 ttttagattg aaacagtttt tgagtataaa tttgtagaat gatttcattt aatcgaccga
     6901 tggttttagc tgatttgtaa taatcctgca taaattgttc aacacctaat aggtgttgtt
     6961 catcatgata atcaaattgt tttgctaacg ttctttgata gtcgaaaagt aaacggtctt
     7021 ctttcctttg agttaatgca tgtaatgcat atctaataga gaacaaaaag tctctagcat
     7081 gaatcaacat tgataactgt tgatttgaaa taattcctag tccattgagt tcttgtaatt
     7141 gttggatgcc aaggattttt tttccaaccc agaaaataga gtggatatct ctcaaaccac
     7201 cagggccact cttaatattt ggttctagat tataggctgt attgttaaat tgtgaatgtc
     7261 tggaaagttg ctcatttttt tttccttcta gaaattgggt gttagaccat aataatttat
     7321 tgccggtaat ttctttttct agtgtttggt atgtggttat tgatccatac aaataccgag
     7381 cttccatcaa tgaagtggca acagttaagt cttttgaacc ttctttcaag cattcttgga
     7441 gagttctagt gctatgacca acttggatgc ctatatccca taaaaaagtt aaaaactttg
     7501 caagactatc ttgaaatgat tttttctcat ttttctcaat tagtagtaga agatctgtgt
     7561 ctgaatatgg atggagttca tttctgccat agccaccaac agcaattaca ttaatacttt
     7621 cttggttctg tttaaaaaat atttctgctg cagatatcac tatagaatcg ttgagttttg
     7681 atcttgccaa aagcaggtga gagatgtgat gaatagagaa tgatgtatct agaaaatctg
     7741 atttttcttt gataaatttt ttgtaatttt taaccgtaaa cggatgcgag tttagtgtat
     7801 ttattaaagt ttctgtcttg aactccttgt atttttcagg attttcaatc gacattgatt
     7861 aaacaatctt ttcgttaggg gatagcgtta agacttcaaa cccatccttg gtaacgagaa
     7921 tggtatgttc ccattgagca gagagcgagt gatctcttgt gacaacggtc cattggtctg
     7981 gcatcaattt aaccgatggt tcaccggcat taatcatcgg ttcaatggta aaggtcatac
     8041 cttctttgat ttctatgccc tggtgtggaa tcccgtaatg cagaacctgt ggttctgcat
     8101 gataagtctt tccaattcca tgaccgcaat attctttgac gactgaataa ttatgttttt
     8161 ctgtatgcaa ttgtattgca tgaccaatat cccccagttg atttcctggt ttaacttgtc
     8221 taattccctg acacagtgct tcataggcta tctttgaaat acgctcgccc tgttgggtag
     8281 gtttgccgat caaatacatc ctgcttgtgt caccgtaaaa actgttttta ataatagcta
     8341 catctatgtt gaggatatcg ccatgtttca atttcttcgt gctcggtatc ccatgacata
     8401 cgacatggtt gacagaagtg caaatggtgg cgggataacc tttatagccg acgttagcag
     8461 gtatagaatt ctggtcattg acaatatagt catgacaaat ctgatccagt tcccccgttg
     8521 tgatccctgg tttaatatga ttttcaatca tgtctaacac ttctgaagct aatttcccag
     8581 aaattctcat ttgttcttgc tcgtctttat ttttaatatc aatggacatt ttcagtaaaa
     8641 attattattg ttagtcggct ttattgtcgc ttagacatta ttatgttata aaacatcggc
     8701 aattttgcat aaattcgaac ataaatcgac acatatattg ggtgctgacc acggattgga
     8761 aagtttatat atgggatttg tgggagtgac aacccaataa ggaataaaaa tggcaacaat
     8821 aacaatgcgt cagatgcttg aagctggcgc ccacttcgga cacaaaacga ggtattggaa
     8881 tcctaagatg gcccaatata tttatggcca acgcaatggg atccatatta tcaacctaga
     8941 aactacgctt cctttgcttg aagaagcttg tctatttatc gagaatctag cagcggaatc
     9001 tgggacagta ttgtttgttg gcaccaaacc tgccgtaaaa aatgagattc tgaatgcagg
     9061 taaggaatgc gagatgcctt atgtttccaa taggtggttg gggggggctc tcacaaataa
     9121 taaaactata gctcaatcca ttaaaaaact tgaagatcta gagatatctc tcaaggatga
     9181 aaatttatca aagaaggaag cactgactat aagaagagct tctgagaagc ttaatagaag
     9241 tttaggtggc attagatcca tgaataggct tccggatgtc atatttatta tagatgtgaa
     9301 ttatgagaag aatgctgttc aggaagcaaa agcattaggt attccgatta taggtatagt
     9361 ggatactaat tcatcgccgg ataatattga ttatattatt ccttcaaatg atgattccag
     9421 aggtactgcc aaggtcatta cagcaagcat tgttgaatca tttttaaagg gtaaaaaatc
     9481 aatacctatg ttggatgata ttgatgatga cttcgtcgaa gttgatgccg aaggaaaaat
     9541 ttcaaaagat cagattaaac ggaaaaagag aatttttgta gcggataaaa aaataactaa
     9601 gaaacaagct tcagaagaca aagttgagag tgatgatgca tcagagagtg acaagatcga
     9661 cgatatcgga gagtcagaaa atctttatat cagcacagaa gaagaacaaa aaaatcaaga
     9721 cagagattca gatcaatcaa acgatccctc gaatagtgaa ccatccgttg aggaaaaaac
     9781 aaagcctggg tcagaacttc caggatctag tgaatcagaa gatgacgaag ataaaaagga
     9841 ttcctaatag cacgggttag aagatgaaaa tttctgccat cgaagtgaaa aaactgagag
     9901 cactgaccaa tgccgggatg atggattgta agaaagcatt ggttgaatct caaggggatt
     9961 ttcagaaagc aatattagtt ctaaaagaga gtggtcagag taaagctgat agtaagtcag
    10021 gtagggtcgc tgcccacggt atagtgtcaa tagaaattga aaaagcacaa cgtttagcac
    10081 tcgtactgga aattaactgt gaaactgact ttgctgctaa agataatcaa tttgtagaat
    10141 tttcaaatca aatttctaat ttattagtca atgaaaaaat tgaaaatatt gatcaactca
    10201 tggattgttc aatttctaaa tttgaaaggg tcgaagattt tcgaaaatct gttgttgcta
    10261 aattaggaga aaatataacc atcagaagat ttgtaagaat attttatcaa ggaactcttg
    10321 gcagttatgt tcatggtaat aaaatagcag ctttagttga tgtggatatt gatgataaga
    10381 atctagcaaa ggatctagcc atgcacgttg ctgcttccaa acctaaatac atagttcaaa
    10441 cagatattcc caaagcaatt attgatgaag agtctaggat attgagatca caggctgaac
    10501 aagaagggaa acctaaagaa attattgaaa aaatagttac aggtaaactg aataaacacc
    10561 taaatgaaat aactttgctt ggtcaggaat atattaaaga tcctgactta aatatttctc
    10621 agttattaaa aaaatccaat gcaactgtaa atctttttgt aagatatgag gtcggagagg
    10681 gaattgagca ccaagataaa aattttgctg atgaagtcaa agaacaagtt gaagcaagta
    10741 aataataatt tttattctct ttgatcaaga atttttatgg ttttttaatg tggtaaataa
    10801 acagataaaa agaatccttt tgaaattaag tggtgaggca ctcctgggca agaaggagtt
    10861 tggtgttgat ccagatgtat taatttatct ggccaaggaa attaaatcta ttctggattt
    10921 aggtactcaa gtaggaattg tgattggagg gggtaatatt tttagaggtg aaggtttagc
    10981 ccagtcagga attgatagag tcactggaga tcaaatgggt atgctggcaa cgattataaa
    11041 tgcattagcc attcaggatg ctttagaacg acaagggctg gtcgcaagag ttatgtctgc
    11101 attaaaagtt aatgaagtat gcgaagacta tattagaaga cgagctatta ggcatttaga
    11161 aaagggcaga gtgactatat tttcagcagg tactggcaac ccttatttta ctactgattc
    11221 tgctgcatca cttagagctg tggagattga agccgatcta ttgattaaag caacaagagt
    11281 caatggggtc tattcagaag atcctttaaa gaatcaggat gcaaatcatt ttgaaacaat
    11341 ttcttttgac aagtttatat caaaaaacct taaagtcatg gatactactt ctattgtcat
    11401 gtgcaaggaa aataatctac caattagggt ctatgatatt aatatcgata atgcattatt
    11461 ggatatagtc caaggtaaga gcgtggggac cttgataatg taatcaagga tgaaagtttt
    11521 gctggaagag attaatcaag aaaccaatga aagaatgcat aaatctattg atgctttttc
    11581 agtgaattta accaaaataa gaacaggacg tgccacaccg gatatcctta atagtatcca
    11641 gattgaatat tacggcaatg atacaccgat acaacaatta tcaaatataa ccgtagaaga
    11701 ctctcaaaca ctcaccattt ctccctggga agaaaaattt attccagaaa ttgagcgtgc
    11761 aatattaaaa gcaaatattg gtttaaatcc agtaacaaca ggaaatatta ttaggattcc
    11821 tttacctact ttgacagagg aaagaagagt agagctaacc aaacaagtaa gaagtgagga
    11881 ggaacaggcc aagatttcta tcagaaatat aagaagagac tcaatccagg atctaaaaga
    11941 tttttctaac gagaagatga tctcagaaga tgatttgcac aaaggccaaa cggaagttca
    12001 agtcattaca gatgagatga taaaaaatat tgaatcctta agtgaacaaa aacaaaaaga
    12061 attaatgagt ttttaaatca tgaaagaaaa tcccacagag gaaaacgttc caaaaattct
    12121 taacctgccc agacatttgg gggttattat ggatggtaat ggtcgttggg caaaattaag
    12181 aaggaaaaga agattctttg gccatgaagc tggtgtttta aaggctaaag agattgttga
    12241 aaactcggtc aaattggaga tagaggccct aactctattt gccttaagtt cagagaactt
    12301 ggacagacca gctagggaag tcgtcagttt gatgcgattg tttgaaagat ccattgaaaa
    12361 atatactggc cttttgatcg agaataatgt tcgttttaaa caaagaggca ataaagaaat
    12421 gttgcccaag gctttggtta gaaaaataga gcaaatagag gctatcacat cagaaaactc
    12481 cggtttaaaa ttgactttgg caatagcata tggctcaaga caggatatta tcagaggcat
    12541 attggatatc attgatggga tagacaaagg tgaaattaat aaagatcaaa tagatgaatc
    12601 aactctctca agctatcttt ctttctcaga tctacctgac ttagatttga tcattagaac
    12661 atcaggtgag actagaataa gtaattttat gctgtgggaa atggcatata gtgaaatcca
    12721 ttttaccgaa acactctggc ctaattttga aaaccaggaa ttttttgaaa tattagaaaa
    12781 ttactcaaaa tcagaaagac gttttggagc aattgaggaa gaaaaagagc tgaaaaaagc
    12841 ctagagcata actaatgaaa aacattacta agagaatctg ttcggcagca gttcttattc
    12901 ttatactcat atacctaact caggattcaa cagcaaacct tgccaaaatt gtagttgcta
    12961 cattactttc tctcattatc gtttgggaat attcaaggtt atttaaatca cgaatctatc
    13021 aagcactttt tcagatactg tttgttagtt ttgttttgtc agcaccttat ttaactggaa
    13081 ttttggagaa aacaagtaac tatttctttg gattgttagt tttcgcatgg gttgtcattt
    13141 tgttcagggt attgacctat aaaaagaaga atattgatga ctttttagtc tttatttttg
    13201 gatatttaac cctacttcca tttgttggat cattgtttac tattttaatt tatcctggaa
    13261 ttttattata tgttgttatc accgtttcaa ttgcagattc atcagcttat ttggtaggaa
    13321 ggaaggtagg taagactgct ttactaccaa atatcagccc tggtaaaacc atcgaaggtt
    13381 ttgttggaag ccttataata actcctataa tctcaagctt aatcgtttat cttttttatg
    13441 gtgaagttgt gattggattt ttatttatcg gattttttgt aacactcata tctgtaattg
    13501 gtgacctatt ttttagttta ttgaaacgta ataaaaatat taaggattcc agtaatttaa
    13561 tacccggtca tggaggtttc attgatttat tagacggtac aatagcggtt cttcccttac
    13621 tcgcattttt attttttaaa gtgagttatc taagcatggt tctttctgtt ataagttttt
    13681 agttaccggt atttataatt tttatctctt attacttcct ttgctgtgct gattctttat
    13741 aatttcttca taatttcaat cgataattag atatatggat tttttattct caattgttgc
    13801 attcatagtc ttggttggcg tactcgttgc catccatgaa tatggacatt atctggtagc
    13861 aagactttgc aatgtcaaag ttctaaggta ttccattggc tttggtaaag tactgtggag
    13921 taaaaacata ggcagagacc aaacagagta ttgtttatct gctattcctc ttggaggtta
    13981 tgtgcaattg ctcgatgaac ggagtgaaga agttcatgaa agtgaagtca aacgtgcttt
    14041 taacaatcaa tcagctgtga agagaatttt tattttatct gcagggcctt ttgctaattt
    14101 tcttttttca attgtagttt atatgactat gttctctatc ggtgttccag gcgtagttcc
    14161 tatcatagga gatgttgaat cagattctat tgcctggcag aacaatttaa gatctggaga
    14221 taagatcatc aatgtcggtg gtaaagatgt caaaacatgg caagattctt tgatttcaat
    14281 gatcggtgat attttagacg acgaacaaat ccagatcata gcaatcgatc aattaggtaa
    14341 tcagaaaaca gttgtattgg atgtcacagg aagaaccaag gagttgacct ctccaaatac
    14401 tttaatgcct ggtttgggat ttaaaccctt tacaccaaag atcaagccca ttatagaatc
    14461 ggtcgatcta gatggaccgg cagataaggc aggtcttatg tcgggagata tcattctaaa
    14521 agttaatgat atcgatatca aaaactggaa ccattttaga gaaatcatca ttgccaatcc
    14581 aaataaagag attgatattg agatcaaaag acttgatcaa gtgtatcgaa caacagtgct
    14641 tttggattct aggactgatg atgtaagtca gggttttata ggactccgag caggaattga
    14701 tgaggaagaa atcaagaagt atcaggctat tcaacaatat aatttcccta aaaatttaac
    14761 aatggcattg gatcaaacca atcagatgat cgttatgacc ttaaatatgt ttggcaaaat
    14821 ggttaccggt caaatttcag ggaaaaatct cagtggacca ctcggaatcg ccaaggacgc
    14881 aggaacagtt gccaaaagag gacttattgc aactttatct tttatggcaa ttattagcat
    14941 tagtttgggt attctcaatc tatttccttt gcccattctg gatggaggac aaatcctgtt
    15001 tgttctaata gaaaaaatca ttcgaaaacc tattccagaa aaaatacaaa taatttttca
    15061 gcaaattgga gtaggagcac tattattctt aatggtcttt gcggtataca atgatcttgt
    15121 aaggatattc agttaatagg actcaatcaa aactatgaaa atcagattaa taattattgc
    15181 tttggctttt ctttgttttt attcagttca agctgaagag tcgttcgttg tcaaagacat
    15241 tcgaattgaa ggtttgcaga agatttctga aggagcgtta ttaaattatt tgcctattaa
    15301 cattggcgac atacttgatg atgttaaagt taaagaatca ataagatcag tctattcaag
    15361 tggttttttt aagaatattg aatttagaca agatgaatcc ggcattttga ttatctcggt
    15421 cctggaaagg ccgtccatag gtaatattgt gtttgatgga aacaaggata taaagacaga
    15481 agatctggag gattcattaa ataacatagg ttttaaagtg ggacgaacat acgacccttc
    15541 tgttttggat gagatcgaga gatcaatgac agatcaatat ttcagtttcg gtaaatacag
    15601 cgctcagatc gaaacgaaag tagatgatct accaggcaat acggtgaata tacaagtcaa
    15661 tattaaagag ggtgatcgag ctcaaattag gcagattaat attgttggga atactattta
    15721 caatgatgag gatctactgg atcaattaac attacagatg ccagattggt tgtcattcat
    15781 tcaacaagat gatcgctatt ctagagaaga tttgcagggt gatttagaaa caatcgaatc
    15841 attttatttg gatagaggct atgcaaattt cagacttgac tctgcccagg ttgccatttc
    15901 accagataga accggtatat ttattaccat caatattaca gaaggggagg tgtatcaact
    15961 ttcagatgtc aagttgactg gtgaatttgt tattcctaaa gaagagattg aacagcttgt
    16021 ttttgctcaa ccgggtcaat ttttttcaca agcactatta actcaagctt cagaattaat
    16081 taaatataga ctcggagaag atggttattc ctttgctgaa gttgaagcaa ttccagaact
    16141 ggatcgtgaa aacaaggaag taaagatcgt tttttacatt cagccaaaaa gtagagtcta
    16201 tgttaggcgt gtcaatttct ccgataccac atccattaat gacgaagttt tgcgacgtga
    16261 actaaggcag ttggaaggtg cttatatgtc gaaaagtaga ttggaacgct caaaagttag
    16321 attgcaaaga ttgccctata tagaggaagt ggaatttgac acaattccag ttgcaggaac
//
LOCUS       DASCTH010000079         1747 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000111720, whole genome shotgun sequence.
ACCESSION   DASCTH010000079 DASCTH010000000
VERSION     DASCTH010000079.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000111720"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="QGH35_01705"
     CDS             <1..593
                     /locus_tag="QGH35_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJL81282.1"
                     /translation="LLLIGYVLTYIFETDEPVLVVLVVIFMIAMSSWIALRPLADRGI
                     KPYSVVTIALGVSGLGVLLLVTQIILELPRWFEPRFVIPLAGMVFANSMNTISLAGER
                     FHAELERGDNYLSARRAAMEVAMIPQVNALLAVGLVSLPGMMTGQILSGVDPLTAARY
                     QIMVMCMIFSTAGLSAVLYMILKKGDITTQLDNKTD"
     gene            706..>1747
                     /locus_tag="QGH35_01710"
     CDS             706..>1747
                     /locus_tag="QGH35_01710"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HJL81283.1"
                     /translation="MKKQIFLTLSILLLAGSVNGVEQKKDLDARIKSINSKFIFADMH
                     AHPSRFHRSNIDSISEEEIHRYKVHNINVVVASISTDMAYTGNYDTENGTVPEGQYKP
                     ASGQSFALTLERLQRIQNTFTKGRAVHADSPSLLLKARDEGNFAIIPAMEGADGLEGD
                     IENLYRFYEMGLRLIQLVHFRANALGHIQSHPYSPGGLTKFGEKVVEEANRLNLIIDV
                     AHANTETIKDVLEISKDPVIFSHGGLKALRDQDRALTDEEVILIAEKGGIVGIWPHGR
                     YIESVTRMVDYIDHVIDLVGPEHVGIASDLRGVSKYSKGFGREANFSAIAEEMILRGH
                     DDETVGKVMGGNF"
BASE COUNT          482 a          320 c          422 g          523 t
ORIGIN      
        1 agcttttgct gattggctat gtgctcacct atatttttga gactgatgag ccagtgcttg
       61 ttgtgttagt ggtgatcttt atgatcgcta tgtcatcatg gattgcacta cgcccgttgg
      121 cagatcgcgg tatcaagcct tattccgtag tcactattgc actcggtgtg agtggattag
      181 gcgtattgtt actcgtcaca caaattatcc tcgaacttcc acgttggttt gagccacgtt
      241 ttgtaatccc actcgccggg atggtgtttg ctaacagcat gaatacgatt agcttggcag
      301 gagagcgttt tcacgctgaa cttgaacgag gcgacaatta tttgagtgct cggagagccg
      361 cgatggaggt agcaatgatc ccacaagtta atgcacttct ggcagttggt ctggtatcgc
      421 taccgggtat gatgaccggc cagatcttgt ccggggttga ccctcttact gctgcacgct
      481 atcagatcat ggtcatgtgt atgattttta gtaccgcagg cttgtccgcg gtgttgtata
      541 tgattctcaa aaagggtgat ataacgacgc aacttgacaa taaaaccgac tgatgtggtc
      601 atcagtagct tgttacgctc ttctagtttt taatcaagtt tcttcagaat tcaaaccatc
      661 atcaagaatt tgaggtttgg ccaatttgct atatagtttt cgggtatgaa aaaacaaatc
      721 tttctaacct tatcaatctt gctccttgct ggttcagtta atggtgtaga gcaaaagaaa
      781 gatttagatg caaggatcaa gtcaatcaat tctaaattta tctttgcgga catgcatgcc
      841 catcccagta gattccatag gtcgaatata gatagcattt ccgaagaaga aattcatcgt
      901 tataaagtac ataatatcaa tgtagtggtt gcttcaatca gtactgatat ggcttataca
      961 ggtaactacg atactgaaaa tggaacagtg cctgaaggac agtacaagcc tgcttctggg
     1021 caatcttttg ctctaacttt agagcgtttg caacgcattc agaatacctt tacaaaggga
     1081 agggcggtgc atgcagattc gccatccctc ctattgaaag caagagatga aggtaatttt
     1141 gcgattattc ctgcaatgga aggtgctgat ggtcttgaag gcgatataga gaatctctat
     1201 agattttatg agatggggct caggctaatt caattggtgc attttagagc gaatgcgtta
     1261 ggtcatattc agagtcatcc ctacagtcca ggcggtttaa cgaaatttgg agaaaaggtt
     1321 gttgaggaag ccaatagact gaatctcatt attgatgttg cccatgcaaa tactgaaacc
     1381 attaaggacg tcttagagat ttcaaaggat ccggtaattt ttagccacgg aggccttaaa
     1441 gcacttagag atcaagacag ggccttgaca gacgaagagg ttatcttgat agctgaaaaa
     1501 ggtggtatcg taggcatatg gccccatgga cgatatatag aatctgttac taggatggtc
     1561 gactatattg atcatgttat agatttggtt ggcccagagc atgttggaat tgcttctgat
     1621 ctcaggggtg tttcaaaata tagtaaaggt ttcggtcgcg aagcgaattt cagtgcgata
     1681 gctgaagaaa tgatactcag aggccatgat gatgaaactg ttgggaaagt tatgggagga
     1741 aatttct
//
LOCUS       DASCTH010000080         6194 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000113347, whole genome shotgun sequence.
ACCESSION   DASCTH010000080 DASCTH010000000
VERSION     DASCTH010000080.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000113347"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            232..861
                     /locus_tag="QGH35_01715"
     CDS             232..861
                     /locus_tag="QGH35_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81284.1"
                     /translation="MFVFWLGTDMGVMLCSKKSIDSTLSIETRFQLLEMALKIELLPR
                     VMWVMALPLGVHLSASLGYIDPSLITLTLMWVFAIAWLIINVGGAANLDKPWGQQLSK
                     LNRFITASLGVGLIIVAISSFMGFGPYEANSIALKVGLYGLVNITILGIEIAFFPLGA
                     SFERLATEGSSPELEQSISSGMGTTLMWVYITYIMIFVVAFIGVAKIAG"
     gene            879..1988
                     /gene="mutY"
                     /locus_tag="QGH35_01720"
     CDS             879..1988
                     /gene="mutY"
                     /locus_tag="QGH35_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HJL81285.1"
                     /translation="MARNQYSYLANQLINWWQQSGRKTLPWQINPSKYKTWISEIMLQ
                     QTQVATVEPYFVRFMERFPTVIELASSSVDEVLQLWSGLGYYARARNIHRSAQFIVNQ
                     HQGELPSDYDSLLTLPGIGPSTAGAILSLSGIEPKPILDGNVKRVLSRFFGVKGWPGE
                     GKVSKELWDLSAKSLPADNFQIYTQGIMDLGATVCLPKNPNCHICPIISRCYAHQHHL
                     VDEIPKKKPITKKRTESKYLLMLIFDGRSNTKDLFLEKRIGQSLWGGLWSLPEIELSE
                     DPRSWCERHFTNTILSIDPWEPIKQPLSNYILYIHPVRIRMDGRLNYMHTSCHPRAFP
                     KPKLNSGCFALTKTLEANFGFPGPVNRLVNQLTKQ"
     gene            1985..2260
                     /locus_tag="QGH35_01725"
     CDS             1985..2260
                     /locus_tag="QGH35_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HJL81286.1"
                     /translation="MTRLVFCEYLQKELEGLDRPPYPGGIGEKIYQSVSKQAWDIWVE
                     HQTMLINENRLSMLDPKSRKYLQEEMAKFLFGKGSVKPSEYIPPDTK"
     gene            2315..2390
                     /locus_tag="QGH35_01730"
     tRNA            2315..2390
                     /locus_tag="QGH35_01730"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2348..2350,aa:Phe,seq:gaa)
     gene            2799..3020
                     /locus_tag="QGH35_01735"
     CDS             2799..3020
                     /locus_tag="QGH35_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81287.1"
                     /translation="MNRIKGWIGELTDIAVALVALGVAAGVVFGMDVPFVGAVLGNLI
                     DLINTLGDNGVVGIIVLAILSGMYGRSGM"
     gene            3122..3733
                     /locus_tag="QGH35_01740"
     CDS             3122..3733
                     /locus_tag="QGH35_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81288.1"
                     /translation="MFRSFLTQLTRVTDTMIIIASALVVLAFLTAPEDLSLEQNAKTS
                     VAAVTSARASLDNAEATVKKASAATAAARNTLAIALELETSLASTDIASADELSSASA
                     ATAAARSTLATTEQALKDAIETTTTASANITAAESTAAAEIAVAESAALRSVWWLQPI
                     RQNTMTLVNAAGIDGFIAVFAALIFCLAYKRRKSWLQKIWFRR"
     gene            3782..4687
                     /locus_tag="QGH35_01745"
     CDS             3782..4687
                     /locus_tag="QGH35_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJL81289.1"
                     /translation="MKTQHFGEISVQKVLDGIENFPATIAFPEINLDLFHQHRDWISP
                     FYDFDSETILISMHSYIIKTPKMTAVVDTCIGNDKYRIGDGPIYKANKDVLSHWNLRN
                     SSYLDNLISAGVHPEEVDYVMCTHMHADHVGWNTKLENGKWVPTFPNAKYLFSPIELA
                     AMQKQTGNPFDEYKGLVFEDSVLPVIESGQSVMMDNDLDLGRGINILPSPGHSPGHYC
                     IELQSKNSQGIITGDIFHNPIQMNYPHWTTMFCDNKEQANETRIKLVDDLIDSETIVL
                     AAHFAGATAGKIISDSNNKKFILLD"
     gene            4696..5100
                     /locus_tag="QGH35_01750"
     CDS             4696..5100
                     /locus_tag="QGH35_01750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HJL81290.1"
                     /translation="MSEPNLNTDNNNCFVCGPNNPIGLRINFLIDEEVCRGEFTPKAD
                     HVGFNNITHGGIIFSLLDDVMANWLFLKNQAAHTAKCELRYRNPLPIGEKVLLEGRCI
                     KRKGRVAVMKGLVIHEQTKKIIAESDAKFLII"
     gene            5240..>6194
                     /locus_tag="QGH35_01755"
     CDS             5240..>6194
                     /locus_tag="QGH35_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HJL81291.1"
                     /translation="MKNKIKQLFQKSKRTLMLIAFTFLFVVYLTLTKTEPELVIIPEK
                     VWPVAAISVEYKDVTPNLELFGEITSARRSELRVSVGGQVVEVGENFKKGGVVKKGEL
                     LLIIDDFEYQNAVIEEQAKYEILNRDFERAEELYLKNSISEQFRDNAMLEKTQQEITL
                     AEAQKDLRDTKLFAPYDGVINGVEASLGKQVSTFNDKVGEIIDIKNLEVRFSLSKAQY
                     GRLLEDDQAVIGRAVAIHWIAGNQIFDFEAEISRTGAEIKSNTGGIDIFATLFFDESE
                     FSLIRPGAFVRLSVTDKTYNQVISVPNTAVYDDEYIFIILND"
BASE COUNT         1948 a         1095 c         1249 g         1902 t
ORIGIN      
        1 ctagaatcat agaactgtct caaaaacagc tcgatgagtt tgaagaaaat aaagattcag
       61 atgattttat gcaaacactt gcatggaata ttggcgagtt atattgctgg caagaagatg
      121 gtgaaattat tgaaccaccc gtacaagtaa actgagatta attcttataa ttctatagca
      181 tgaggttgtc ttatggattc atatgagttt tggaaattcc tgcatatatg catgtttgtt
      241 ttttggcttg gcacggatat gggagtgatg ctttgctcca agaaatcgat agattctact
      301 ctcagcatag aaacacgttt tcaattatta gaaatggcct taaagataga actgttacca
      361 agggttatgt gggtcatggc gttgccattg ggggtccatt tatctgcatc gcttgggtat
      421 attgacccca gtttgataac gctgacttta atgtgggttt ttgctatcgc ttggttaatc
      481 attaatgttg gtggtgctgc aaacttggat aaaccatggg gacaacagct atcaaagcta
      541 aaccgattta ttaccgcatc tttgggcgtt ggcttaatta ttgttgctat atcctcgttt
      601 atgggttttg gaccctatga agccaactct attgcattaa aagtgggtct ttatggtctg
      661 gttaatatta cgattttagg gatcgaaatc gcattttttc ctttaggcgc atcatttgaa
      721 agactggcta cagaaggctc tagcccagag ttagaacaat cgataagctc aggcatgggc
      781 actactttaa tgtgggttta catcacctat attatgattt ttgtagtggc ttttattggc
      841 gtagccaaaa ttgcaggtta gtgtcaacag ccttttttgt ggcacgcaat caatacagtt
      901 acctcgctaa ccagttaatc aattggtggc aacaatctgg aagaaaaact ctcccctggc
      961 aaatcaatcc atccaaatac aaaacttgga tttcagaaat catgttacaa caaacccaag
     1021 tagctactgt tgagccttat tttgttagat ttatggaacg atttcccaca gtaatagaac
     1081 tggcatcatc ctctgtagat gaggtcctac aattatggtc agggcttggt tattatgccc
     1141 gagctagaaa tatacatcga tctgcacagt tcatagttaa ccaacatcag ggtgaactgc
     1201 cttcagatta tgatagtctg ttaactttac caggcatagg tccatcgaca gcaggtgcta
     1261 ttttatccct cagtggtatc gagccaaaac caatactgga tgggaatgta aaaagagttt
     1321 taagccgatt cttcggtgtt aaaggttggc ctggtgaagg caaagtcagc aaagaattat
     1381 gggatttatc tgccaagtct ttacctgctg acaactttca aatatacaca caaggcatta
     1441 tggatttggg tgccacagtt tgtttaccaa agaatccaaa ttgccacatc tgtccaatta
     1501 taagccgctg ttatgcacat cagcatcact tagttgatga aattcctaag aagaaaccta
     1561 taacaaagaa gagaacagaa tcaaagtatc tgcttatgct tatttttgat gggaggtcta
     1621 atacaaagga tttattccta gaaaagagaa tcggacagag tttgtgggga ggtttatggt
     1681 ccttgcctga aatagagctt tcagaagacc ctagatcttg gtgtgaacgg catttcacca
     1741 ataccatcct ttcaattgac ccatgggaac caattaaaca accactcagc aattacattt
     1801 tgtatataca cccagttaga attaggatgg atgggagatt gaattacatg catacatcat
     1861 gccatccaag agcttttcca aagcccaaac tgaactcagg atgttttgcc ttaactaaaa
     1921 ccctggaagc taattttgga tttccgggtc cagtcaaccg gttggttaat caactgacta
     1981 agcaatgacc agactagtat tttgtgaata ccttcaaaag gaactggaag gattagaccg
     2041 gcctccatac cctggcggga taggagaaaa gatctatcaa tctgtttcaa aacaagcatg
     2101 ggatatttgg gtagaacacc aaaccatgct catcaatgaa aatagactat caatgttaga
     2161 tccaaaatcc agaaaatatt tacaggaaga aatggccaaa tttttatttg ggaaaggctc
     2221 tgtaaaacca agcgaataca tccctcctga tacaaagtga ttttttgtgg ttttgtttta
     2281 attattctat aaaatgaacc gacgcaaaga ccggggccag atagctcagt aggtagagca
     2341 gcggactgaa aatccgcgtg tcggtggttc gattccacct ctggccacca cgccttctag
     2401 aaatccaata ccaagtgttc aattagaaag ctaaacacat attagaaatt aaaacgttgt
     2461 aagatttgat tttagaatga gtctaaaaag cgggataatt attacttaac tcaactttat
     2521 tcattttttt tgcaaaaaag ttttcaatat tccctaataa tctttatatc aagggtattg
     2581 aactgtgttt tactttaatg ttaattgctt gttgtgattc agtcttttgt gaataactca
     2641 ttgattacat agagatgcac agtttttgtt atttgttgtt ataaaacaaa tatagtccag
     2701 ttctttgact aatgtaactc attttgatct gattcctgat gttcttttga gaaagagaac
     2761 aaacttaaac aaacctaatt ttaggggagg attttatcat gaatagaatc aaaggttgga
     2821 ttggagaact gactgatatc gcagttgctc ttgtagcact tggtgttgca gctggcgtag
     2881 tatttggtat ggatgtacca tttgttggcg ctgtcctagg taacttgatc gacttgatca
     2941 atacccttgg tgataacggt gttgttggaa ttattgttct tgcaattctc tcaggaatgt
     3001 acggccgtag cggtatgtaa atgagaacaa gcttttaata taaaagcaaa aagggctctt
     3061 tttttaaaga gccctttttt tatatcttta ttttgtaagc gtcctcaact attcttaagt
     3121 catgtttaga agtttcttaa cccagctgac aagagttacc gatactatga tcattattgc
     3181 atcagctctg gtcgttttgg cttttcttac tgcaccagag gatctatctt tggaacaaaa
     3241 tgcaaaaaca tcagttgcag ctgttacctc tgctagggct tctttggata atgctgaagc
     3301 aacagtaaaa aaagcatctg cagccacagc agctgccaga aataccttag ctatagcctt
     3361 agagctggaa acaagcctag ccagtacaga cattgcttcg gctgatgaat tatcgtcagc
     3421 atctgcagcc acagcagctg ccagaagtac tttggcgact acagagcaag cactcaagga
     3481 tgcaatagag acaactacca cagcatctgc aaatataaca gcagctgagt caacagctgc
     3541 agcagagata gctgtagccg aatctgcagc gttaagatcc gtttggtggc ttcaacctat
     3601 taggcaaaat actatgactc tggtcaatgc tgcaggtata gatggtttca ttgctgtgtt
     3661 tgctgcatta attttttgcc ttgcatacaa acgaagaaaa tcttggctcc aaaaaatttg
     3721 gtttagaaga tagtattcaa ttattgtagg cgggcttcga cattgatgat gatcaattct
     3781 tatgaaaaca cagcactttg gtgaaatatc agtacaaaaa gttcttgatg gcattgaaaa
     3841 tttcccggca actatagctt ttccagagat taatctcgat ctttttcatc agcatcgaga
     3901 ttggatatca ccattttatg attttgattc tgaaactatt ctaatcagta tgcattccta
     3961 cattatcaaa acaccaaaaa tgactgcagt agttgatacc tgcattggca acgataaata
     4021 ccggattggt gatggaccaa tatacaaagc taataaagat gttttgtctc attggaattt
     4081 aagaaatagc tcctatcttg ataatttaat cagtgcagga gtgcaccctg aggaagttga
     4141 ttacgtcatg tgtacacata tgcacgctga ccatgtgggc tggaacacaa aattggaaaa
     4201 tggtaaatgg gttcctactt ttccaaatgc gaaatattta ttttcaccca ttgagcttgc
     4261 agcaatgcaa aaacaaacag gcaatccttt tgatgaatat aaaggtttgg tcttcgagga
     4321 cagtgttctt ccagtaatcg aatcaggcca gtcagtgatg atggataatg acttagatct
     4381 tggcagaggt atcaatattc taccaagccc tggacatagc cctggacact attgtattga
     4441 gctccaatca aagaactcac aaggaattat cacgggagac atttttcaca acccaattca
     4501 gatgaactac ccacattgga ccacgatgtt ttgtgacaat aaagaacaag ccaatgaaac
     4561 cagaataaag cttgtggatg acttaataga tagtgagact attgtcttgg cagcacattt
     4621 tgctggagct actgcgggta agattatttc tgattcaaat aacaaaaaat ttattctttt
     4681 agattaattt cttttatgag cgaacctaat ttaaatacag ataataacaa ttgtttcgta
     4741 tgtggaccca ataatcctat tgggttgaga attaatttct taatagatga ggaggtttgt
     4801 cgaggagaat tcacacctaa agcagatcat gtagggttta acaatattac ccatggagga
     4861 attatattta gcttgctaga tgatgtgatg gcgaactggc tttttttaaa aaaccaagct
     4921 gcacacactg caaaatgtga gcttagatat cggaatccat tgcccatcgg tgaaaaagtt
     4981 cttttagaag gacgatgtat aaaaagaaaa ggtcgtgttg ctgtgatgaa aggattggtc
     5041 attcatgaac agactaaaaa aattattgct gaaagtgatg ctaagttttt gatcatctga
     5101 tcttaatact atgtaacttt ttgtaactct tacagataat tccaaagcat ttcattagta
     5161 ttcactctgg gtgagtaagt ttgaatatag cgctttcatg gattcgaatt attaattgat
     5221 agtgtggata aatagccaat tgaaaaacaa aataaaacaa ctctttcaaa agtcaaagcg
     5281 aacattaatg ctgatcgctt ttacttttct atttgttgtt tatttaaccc tgacaaaaac
     5341 agaacctgaa ctggtcatta ttcctgaaaa agtttggccg gtagcagcga tatcagtaga
     5401 gtacaaagac gtcactccaa atttagaatt atttggggaa ataacctctg ccagaaggag
     5461 tgaactccgg gtctctgttg gtggacaagt ggttgaagtt ggagagaatt tcaaaaaagg
     5521 tggtgtcgtt aaaaaaggtg agcttcttct catcattgat gattttgaat atcaaaacgc
     5581 agtgattgaa gaacaggcca aatacgaaat tcttaaccgg gattttgaga gagcagagga
     5641 attatatctg aagaattcta tttcagagca attcagagac aatgccatgt tagaaaaaac
     5701 ccaacaagaa ataactctag cagaagcaca aaaagactta cgagatacta aattatttgc
     5761 accctatgat ggggtcatca acggtgttga ggccagtctt ggcaagcaag ttagtacatt
     5821 caatgacaaa gtcggtgaaa ttattgatat caaaaacctt gaagttcgtt tttcactatc
     5881 gaaagcccaa tatggtcgat tattggaaga cgaccaagct gttattggca gagctgttgc
     5941 tatccattgg attgcgggga atcaaatctt cgattttgaa gcagagattt cacgaactgg
     6001 tgctgaaatt aaatccaata ctggtggtat tgatattttt gctacattat tttttgatga
     6061 atcagaattt tctctaataa gaccaggagc ctttgttaga ttgtcagtta ccgacaagac
     6121 ttacaatcaa gttatttctg ttccaaatac tgccgtttat gatgatgagt atatttttat
     6181 tattctcaat gatc
//
LOCUS       DASCTH010000081         1779 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000115748, whole genome shotgun sequence.
ACCESSION   DASCTH010000081 DASCTH010000000
VERSION     DASCTH010000081.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000115748"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="QGH35_01760"
     CDS             <1..558
                     /locus_tag="QGH35_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HJL81292.1"
                     /translation="MKAQMAINERKLLQEDLIPLQQGIRITPKDTNEYMRQLANLAEA
                     KQDLLLARILMNALNRFATTKNVQDVSTSTHTMVESEADRLESELSMIRYIAWAIPSI
                     GFIGTVRGIGAALSLAHRAVDGDISGVTQNLGIAFNSTFIALLISIVIMFLVHQLQLI
                     QERQIFETENYCDQNLISHLKGEQE"
     gene            669..1763
                     /locus_tag="QGH35_01765"
     CDS             669..1763
                     /locus_tag="QGH35_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024909.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HJL81293.1"
                     /translation="MARREFNVFNLSFLDVISCGFGAVILFFIIINAQVKTRSQQASV
                     DLLNETEFLQEEIFDEQKRMVKIKNEIEKTAQDNNIIRGSIQALLDNIEKIVAELSLF
                     KNQTLVANKSINQLKSDVKQLEQENNRMTAESERAKEDPGNFLREFVGEGNRQYVTEL
                     KLSGKHVLFLVDASTSMLGRTYVNVIRYRNMKDEQKVKAPKWRQAVNSVDWLTTRLEA
                     GTKFQIYFFNENAQSIISGTDGQWLEITDGTEIKRAIDRLRGTVPEKGTSLINAFEAL
                     QQLNPRPDNIFLLTDGLPTQGNRSPLRETRVSPEQRIKYFNQALRELPPIPVNVLLFP
                     MDGDPLAAEAFWRLAIRSRGSFMAPSRDWP"
BASE COUNT          592 a          330 c          380 g          477 t
ORIGIN      
        1 atgaaagcac aaatggcaat caatgagagg aaactgctgc aagaagatct cattcccttg
       61 caacaaggca ttcgaatcac tcctaaagac accaacgaat atatgaggca attggcaaat
      121 cttgcagaag caaaacagga tcttttactg gccagaatat taatgaacgc actcaaccga
      181 tttgctacca ccaaaaacgt tcaagatgta tcgacttcaa cgcacacaat ggttgaatcc
      241 gaagcggatc gtctggaatc agaactttca atgattcgtt atattgcatg ggctatcccg
      301 tccattggct ttataggaac cgttcgaggt attggtgcag cattatcact tgcacacaga
      361 gcagtagatg gagacatcag tggcgtgaca cagaatttgg gtattgcctt taactccaca
      421 tttattgctt tgctaatcag tatcgtaatt atgtttctag tacatcagtt gcagttaata
      481 caggagcgcc aaatctttga aacggaaaat tattgtgatc aaaatctgat cagtcatctg
      541 aaaggggagc aagagtaatt ttcctaagat gttgttatcg attcatgcca tggctgatct
      601 agaaatattc gttttattcc taaacaagca acgcactgaa cgccggatat taaagactgg
      661 agacttctat ggcgcgtcgt gaattcaatg tttttaattt atcttttctg gatgttattt
      721 cctgtggttt tggtgcagtt attttgtttt ttataatcat caacgcacaa gtaaaaacta
      781 gaagtcaaca agccagtgtg gatttactca atgagactga atttttacaa gaagagattt
      841 ttgatgaaca aaaaagaatg gtcaagataa aaaatgaaat tgaaaaaacc gcccaggata
      901 acaatatcat cagaggcagt atccaagccc tgcttgacaa cattgaaaaa atagtagcag
      961 agctatcgct tttcaagaat cagactttgg ttgctaacaa gagcatcaat caacttaaat
     1021 cggatgtgaa acaactcgag caggaaaata acagaatgac agctgaatct gaacgtgcaa
     1081 aagaagaccc tggaaacttc ctaagagagt ttgtgggtga gggcaatcga caatatgtaa
     1141 ctgaacttaa actgagtggt aagcatgtgc tttttttagt tgatgcctca accagtatgt
     1201 tgggtcgaac ttatgtcaat gtgattcggt acaggaacat gaaagacgaa caaaaagtca
     1261 aagcaccaaa atggaggcaa gcagtcaata gtgttgattg gcttacgact cgattagaag
     1321 caggaacaaa atttcaaatt tattttttta atgaaaatgc acaaagtatt atcagtggaa
     1381 ccgatggcca atggctagag ataaccgacg gtacagaaat taaaagagca attgatcggt
     1441 tgagaggcac cgttcctgaa aaagggacta gtttgattaa tgcgtttgag gcgctacaac
     1501 aattgaaccc acgacctgat aatattttcc ttctcacaga tgggctacct acacaaggaa
     1561 acagaagtcc cttacgtgag accagggtta gtccagaaca acggatcaaa tattttaatc
     1621 aagcattgcg agaactgcca cctataccgg tgaatgttct gttgtttccc atggatggcg
     1681 atcctttagc agcagaggct ttttggcgtt tagccatcag atcaagaggt agtttcatgg
     1741 caccttcaag agattggccg taatgagaaa acgtcgcag
//
LOCUS       DASCTH010000082         1859 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000116532, whole genome shotgun sequence.
ACCESSION   DASCTH010000082 DASCTH010000000
VERSION     DASCTH010000082.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000116532"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="QGH35_01770"
     CDS             <1..1521
                     /locus_tag="QGH35_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008843668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HJL81294.1"
                     /translation="VLDKIRSIDPATLPESEQLNYRLFLYNYERAVDAQRFDRHLLPF
                     SQRGGIQLEHETAEGLSFQTKQDYEDWLVRLTKLPALISAHIELAKLGIQKGITAPKV
                     LMQRIPKQIRLQMTDDPKKSPFYKVFENISDKIDTQDQLHIQQRALLVIQDQVIPAYR
                     ILDKFFINEYLPAARESYGVYDLPNGYQWYEQLSKWHTTTALSPDEIHQIGLREVQRI
                     RGEMQTIIESLNWEGTFDEFLTYLRTDPQFYFETEEELLQAYLATSKRIDPLLTRLFD
                     TLPRMPYGIKPIPIESAPDTTTAYYMSPAADGTRAGYYYVNLYRPEVRPKYEIEVLSV
                     HEAVPGHHLQIALAMELENIPNFRRFSGYTAFVEGWGLYSESLGEELDLYQDPYSKFG
                     ALTYEMWRAIRLVVDTGMHYKGWSRDAAIDFFKTNAAKTEQDIVNEIDRYLIMPGQAL
                     AYKIGQLKILELKNRAIKELGESFDIKAFHDLILGQGALPLSVLEERVDQYLLELK"
     gene            complement(1507..>1859)
                     /locus_tag="QGH35_01775"
     CDS             complement(1507..>1859)
                     /locus_tag="QGH35_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016777.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81295.1"
                     /translation="YPNTQFILGHSGYDSATVSLTYLDSAITLAKRYDNVYLEPGALG
                     ASKAEKVLPEYLKIIKENGLIGKVIYGSDGPQFPGYTKSHLDRFLTAMQDTGYTAEEI
                     ELLLQTNFEILFEL"
BASE COUNT          578 a          338 c          398 g          545 t
ORIGIN      
        1 gttctggata aaataagatc gattgaccct gcaacattac cagaatcaga gcaattgaat
       61 tatagattgt ttctttacaa ttatgagaga gctgttgatg ctcaacgatt tgatcgtcat
      121 ctattgccat ttagtcaaag aggtgggatt caactggagc atgaaacagc agagggctta
      181 tcctttcaga caaaacaaga ttatgaagat tggttggtca ggttgaccaa gctgccggct
      241 ttaatttcag cccatattga attagcaaag ctagggattc aaaaagggat tactgcaccc
      301 aaggtgttga tgcagagaat tccaaaacaa attagattgc aaatgaccga tgaccccaaa
      361 aagagtcctt tctataaagt ttttgaaaat atttcagata aaattgatac ccaagatcag
      421 cttcatattc aacaacgggc tctgctagtg attcaagatc aggtgattcc agcttaccgt
      481 atcttggata aattttttat caacgagtac ctccctgctg ctagggaatc ctatggtgtc
      541 tacgatcttc ccaatggata ccagtggtat gaacaacttt caaagtggca caccacaaca
      601 gctcttagtc ctgatgaaat tcatcagata ggtctgagag aggtccaaag gattcgaggt
      661 gaaatgcaaa cgatcattga atccctcaat tgggaaggaa cgtttgatga gttcttaact
      721 taccttagaa cggacccaca gttttatttt gaaacagaag aggaattact ccaagcatac
      781 ctggctacgt ctaagagaat tgatccacta ctgacacgat tgtttgatac tttacctagg
      841 atgccttatg gcattaaacc aattccaata gaatctgccc ctgatactac tacagcttat
      901 tatatgagtc ctgctgctga tggcactagg gcgggttatt attatgtgaa tttatatcga
      961 cctgaagtga gacctaaata tgaaattgaa gtattgtcag tccacgaagc tgtgccaggg
     1021 catcatttgc aaatagcctt ggcgatggaa ttagaaaaca tcccaaattt caggcgattt
     1081 tcaggataca ctgcttttgt agaaggttgg ggtttgtata gtgagagctt aggtgaagaa
     1141 ctagatctgt atcaagatcc ttattctaaa ttcggagcac tgacttatga aatgtggcgt
     1201 gcgattcgtt tggttgtgga tacaggaatg cattataaag gttggagcag agatgcagcg
     1261 attgattttt ttaaaactaa tgcagcaaaa actgaacaag atattgtcaa tgagattgac
     1321 cgctatttaa ttatgcccgg acaagcacta gcttataaaa ttggtcaatt gaagatattg
     1381 gaactgaaga acagagccat taaggaacta ggggaatcgt ttgacattaa ggcctttcac
     1441 gatctgatat tagggcaggg tgctcttcca ctgagcgttc tagaagagag ggttgatcag
     1501 tacttactag agctcaaata atatttcgaa attagtttgt agtagcaatt ctatctcttc
     1561 tgcggtatat cctgtgtctt gcatggcagt taaaaagcga tccaagtgac tctttgtata
     1621 acctgggaat tggggcccat ctgagccata aataacctta ccaatgagac cattctcttt
     1681 aatgattttt aggtattcag gtagaacttt ttcagcctta ctggctccca aagcacctgg
     1741 ttctaaatag acattgtcgt atctttttgc caaggtaatg gcagaatcca gataagtcag
     1801 tgaaacagta gcactatcgt atccagaatg accaagaata aattgagtgt ttggatatt
//
LOCUS       DASCTH010000083         7142 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000118526, whole genome shotgun sequence.
ACCESSION   DASCTH010000083 DASCTH010000000
VERSION     DASCTH010000083.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000118526"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            425..1411
                     /gene="ilvC"
                     /locus_tag="QGH35_01780"
     CDS             425..1411
                     /gene="ilvC"
                     /locus_tag="QGH35_01780"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013478234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HJL81296.1"
                     /translation="MKIFSKDDVNQNQLQSKKVTILGYGSQGRAHAMNLKDSGLNVVV
                     GLRQDGSSWSKAIADGLVVKTIEEAVSDSDLIAFLVPDMAQPQIYQSIESGIKNSATI
                     LFAHGFNIHYNQIEPREDLDVILIAPKSPGDLVRREYKQGKGVPCLMAVYKDNSGQAE
                     ADAMAYAHGIGGSRAGILSTTFAEETETDLFGEQAVLCGGCTELIIAGFDTLVEAGYQ
                     PEVAYFECMHELKLIVDLMYEGGIAKMHEFVSETAIYGDLSRGPRVINAETRERMKTI
                     LLEIQNSTFSNEWIEENKSGKENYNQMMADDLNHPIEKVGSELRKRMSWLNK"
     gene            1421..2956
                     /locus_tag="QGH35_01785"
     CDS             1421..2956
                     /locus_tag="QGH35_01785"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017931430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HJL81297.1"
                     /translation="MLKQSENKVLIFDTTLRDGEQAPGCSMTLREKLKIAHSLKELNV
                     NVIEAGFAAASPGDFEAVQAIASEIDGPTICTLARVNPKDIELASQALASNANGRIHV
                     FVATSEIHLEHKLNMARDEVMKAAFEGVKLAKSYCNDVEFSPEDASRTDHDFLIDIVN
                     AAIEAGATTINIPDTVGYTIPGEFRQLFDHLITNCINSEKIVFSVHCHDDLGLAVANS
                     LAALEAGARQVECTINGIGERAGNTSLEEICMAIKTRKECFEFETQIDTKKLYPTSRL
                     VSSITGMHVPRNKAIVGENAFAHEAGIHQHGMLRHASTYEIMKPQDVGIGRSNLVLGK
                     HSGRHAFLDRVNDLGFNLNQEELNEAFIEFKKLADRKKEIFDTDIEAIVMNSESGVAG
                     PWQLLDLETSSGKSWGASAEVKLEHNDGRILETSTQGIGPIEAVFKALEEITGYNMEL
                     TNFEIHSVSIGDDAQGEVTISIRYNDQDYRGHGVSTDIIESGALAYLEVINRIDRKNN
                     KHS"
     gene            2965..3894
                     /locus_tag="QGH35_01790"
     CDS             2965..3894
                     /locus_tag="QGH35_01790"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HJL81298.1"
                     /translation="MSLEPTKHIWFNGKLVPWEDANIHVLTYALHYGTAVFEGIRAYS
                     TNDGCKIFRLHEHTKRLLNSAKIYKIKIPYSQQELEDACRTIINSNDLTDGAYIRPIV
                     FKGYGDLGLHASQDDNIEVAIAAWEWGTYLGEDALNDGVDVCVSSWTRAAPNTIPVLA
                     KAAGNYLSGILITEEAHQNGFDEGIVLDVNGMVSEGAGENIFMVKDNTVYTPPIGSSI
                     LDGITRDSVMKLAESMGIEVKEIQIPREQLYIADELFFTGTAVEVTPVRSVDRITIGD
                     GHKGPVTAQIQKAFFGLFDGSTKDSWDWLEPIQ"
     gene            3902..5314
                     /gene="leuC"
                     /locus_tag="QGH35_01795"
     CDS             3902..5314
                     /gene="leuC"
                     /locus_tag="QGH35_01795"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HJL81299.1"
                     /translation="MAKTLFDKIWEQHVVVPETTNTPAILYIDLHLIHEVTSPQGFTL
                     LRENNLQMRAPQRILATMDHSTPTVPIHSLKDLDIVSAKGPADQIREMIKNCEDFGID
                     LFGLDSEQRGIVHVIGPELGATQPGKTIVCGDSHTSTHGALGALAFGIGSTEVGHVFA
                     TQCLLQRKPKTMAVHLNGKLNMGVSAKDIILKVINSIGVDGGTGCVIEYLGSAVPSLS
                     LEERMTICNMTIEGGARAGLFAPDDATFDYLYGKPMSPKGNDWDQAVTRWNDLQTDDD
                     AVFDKVINIDVDSLTPMITFGTNPGMAMPVDGAIPDHNGNKSVKQALDYMNFESGQSL
                     INHPINTVFIGSCTNSRISDLRDAAAVLKGRKVSENVRVLIAPGSQKVKAAAEAEGLD
                     KIFQEAGADWRESGCSMCIGINGDHLEKGELAVSTSNRNFEGRQGPGSRTILASPKTA
                     AASAIVGSVQDPRQLGVINE"
     gene            5307..5882
                     /gene="leuD"
                     /locus_tag="QGH35_01800"
     CDS             5307..5882
                     /gene="leuD"
                     /locus_tag="QGH35_01800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HJL81300.1"
                     /translation="MSKIIKFSARTVNLPNEDIDTDQIIPARFLAQASTFEDLGKDLF
                     ADWRYEKTGELKQDFILNQPEASEAEVLVTGNNFGCGSSREHAPWALVDFGFKAIIST
                     SFADIFRNNALKNGLIPIVVDRETHHWLLNHPATMVTIDLEAMELSLENGHAAPFPIE
                     PFSRYCLMSDINQLDYILRHNAEITAYENKE"
     gene            5882..6946
                     /gene="leuB"
                     /locus_tag="QGH35_01805"
     CDS             5882..6946
                     /gene="leuB"
                     /locus_tag="QGH35_01805"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HJL81301.1"
                     /translation="MKASITIMPGDGVGPEITREAVKVLQAVETIFDHDFLLKEVLFG
                     GIAIDETGSPYPEETQRSCKSSDAVLLGAVGGPKWSDPNMKVRPEKEGLLEMRAHLGV
                     YANIRPIKTYPQLIENSPIKNRYLDNIDMVFVRELTGGIYFGKKEKTESSASDICEYS
                     KTEIERITRVAANIAQDRRKKITSVDKANVMATSQLWRSTTTELIAEEYTDLELEHIF
                     IDAAAMHLLSRPADFDVILTDNLFGDILTDEAAILTGSIGMLPSACLGENSFGVFEPI
                     HGSAPDIANQGIANPCGAILSAAMLLQYSLNLKEEAQVIEQSVSGVIEDGILTKDLTE
                     SSYVSTEEVGTAVMEKILQT"
BASE COUNT         2317 a         1219 c         1572 g         2034 t
ORIGIN      
        1 ataactgcaa tttttgaagt tactctatga atactgttca tgtaatctcc tcaaatggtt
       61 gtaagtggta acaaaatggg aaacagtaat atcccccact accattaaaa attaaaagat
      121 gatcatatca tattgataaa atactccaaa gagtaaatcc gtacctttta gttataactt
      181 tgtcaaaaat agttgacaaa tttcttctga tcaataacta ttcggaatat ggatgataag
      241 ttgacaagtc taaatgattt aataatttct ctggatctaa ttctagaggg actactcctg
      301 ttagatttaa attactatgg ggctgaaata taaagttaaa aaagcattta attaaatata
      361 agccccgcta acaagcgggg ttttttttaa caattaaaaa aaatgaagta agaggataca
      421 aaaaatgaaa atattttcaa aagatgatgt taatcaaaat caactgcagt caaaaaaagt
      481 aacaatattg ggatatggca gtcaaggaag agcgcatgcc atgaatttaa aagatagtgg
      541 tttaaacgtt gtagtgggat tacgacaaga tggctctagc tggagtaagg ccatagcaga
      601 cggtttggtc gttaagacca tagaagaggc ggtgagcgat tctgatctaa tagcattttt
      661 agtcccagat atggcccaac ctcagatcta ccaatcaatt gagtctggga ttaaaaattc
      721 ggctactatt ctttttgctc atggttttaa tatccattac aaccagatag agccacgtga
      781 agaccttgat gtcattctca ttgccccaaa aagcccaggt gatttggtca gaagagaata
      841 taaacaaggc aaaggtgtgc cctgcttaat ggccgtctac aaagataatt ctggacaagc
      901 tgaagcggat gcaatggcat atgctcatgg cattggtggt tctagagctg gaattctttc
      961 taccactttt gctgaggaaa ccgaaaccga tttattcggt gaacaagcag ttttgtgcgg
     1021 gggttgtacg gaattgatta tcgctggttt tgacaccttg gttgaggctg gttatcaacc
     1081 ggaagtagcg tattttgaat gtatgcatga gttaaaatta attgttgatc ttatgtatga
     1141 gggtgggatt gctaaaatgc atgaatttgt cagtgaaacg gcaatttacg gtgatttgtc
     1201 cagagggccg agagtgatca atgcagagac aagagaaaga atgaaaacca ttcttttaga
     1261 aatacaaaat tcaacatttt caaatgaatg gattgaagaa aataagtcag gcaaagaaaa
     1321 ttacaatcaa atgatggctg atgatttaaa tcatcctatt gaaaaagttg gtagtgaact
     1381 tagaaaaaga atgtcttggt taaataaata ggattcacta atgctaaaac aatcagaaaa
     1441 taaggttttg atatttgata ctaccctgag agacggggaa caggcacctg ggtgcagcat
     1501 gactcttaga gagaagctta aaatcgccca cagcttaaaa gaattgaatg ttaatgtcat
     1561 tgaagctggc tttgccgcag cttctcctgg agattttgag gcggtacagg cgattgcatc
     1621 tgagattgat gggcctacta tctgcacact ggcacgtgtt aatcctaaag atattgaatt
     1681 agcatcgcaa gccctggcat caaatgccaa tgggcgaatt catgtttttg ttgcaactag
     1741 tgaaatccac ttagaacaca aacttaatat ggctcgtgat gaggtgatga aggcagcatt
     1801 tgaaggagta aaattagcaa aatcatattg taatgatgtt gaattttcac cggaagatgc
     1861 atcaagaaca gatcatgatt ttctcatcga tattgtcaac gctgcgattg aagctggtgc
     1921 cacaacaatt aatattccag acacggtcgg ctacacgatt cctggtgagt ttaggcaatt
     1981 atttgatcat ttaattacaa attgtataaa ttcagaaaaa attgttttca gcgttcattg
     2041 tcatgatgat ttaggattag ctgtggcaaa tagccttgca gcactggagg ctggtgctcg
     2101 gcaggttgaa tgcaccatca atgggatagg tgaacgagct ggcaatactt cgctagaaga
     2161 aatatgcatg gccattaaaa cccgtaaaga gtgttttgaa ttcgaaacac agattgatac
     2221 taaaaaactc tatccgacca gtagattagt atcatcgata accggcatgc atgttcctag
     2281 aaataaagct attgttggag aaaatgcttt tgcacatgaa gcagggattc atcagcatgg
     2341 catgctaaga catgcttcaa cttacgaaat catgaaacca caagatgttg gtataggtag
     2401 atccaatcta gtcctaggaa aacacagtgg tagacatgca tttctagata gagtcaatga
     2461 tctaggtttt aatttaaatc aagaggaact gaatgaggct tttatagaat ttaaaaaact
     2521 agctgacagg aagaaagaaa tttttgatac tgatatagaa gctattgtca tgaattctga
     2581 atccggtgtt gctggtccat ggcaactttt ggatcttgaa acatcatcgg gaaaaagctg
     2641 gggtgcaagt gcagaggtga aattggaaca caatgatggc agaattttag aaacttcaac
     2701 tcaaggcata gggcctatcg aagcagtttt caaagctcta gaagagatta ccggctataa
     2761 tatggagttg actaattttg aaatccatag cgttagtatt ggtgatgatg cacaaggtga
     2821 ggtgacaata tctattcgtt ataatgatca ggattatcgt ggacatggag ttagtacaga
     2881 tattatagaa tctggtgcgc tggcttatct agaggttatc aatcgtattg atagaaaaaa
     2941 caataaacac agttaggtga ttaaatgtca ttagaaccga caaaacacat atggtttaac
     3001 gggaagcttg ttccctggga agatgcaaat atccatgttc taacctatgc acttcattat
     3061 gggactgctg tctttgaagg cataagagcc tatagcacta acgatggttg taaaattttc
     3121 agacttcatg agcacactaa acgtttattg aactcggcaa agatttacaa gataaaaatc
     3181 ccctactctc aacaagaact cgaagacgca tgccgtacga ttataaattc taatgactta
     3241 actgacgggg cctatattag acccattgtg tttaaaggct acggcgattt aggtctgcat
     3301 gcatcacagg atgataatat cgaagttgcg attgcggctt gggaatgggg tacctatctg
     3361 ggcgaggatg ctcttaatga tggtgttgat gtgtgtgttt catcttggac tcgcgcagca
     3421 ccgaatacca tacctgtttt agccaaagcg gctggcaact atttatcggg gattcttatc
     3481 actgaggaag cacatcagaa tggttttgat gaagggatcg ttcttgatgt caatggcatg
     3541 gtcagcgaag gtgctggaga gaacatattt atggtaaaag ataatactgt ttatactcca
     3601 ccaatcggct cttctatttt agatgggatt actagagatt cagttatgaa acttgctgaa
     3661 tccatgggca tagaagttaa agagatacaa attccaagag aacaactata tatagcagat
     3721 gagcttttct tcaccggaac cgccgttgag gtgaccccag tgagaagtgt tgacagaatc
     3781 accatcggtg atggccacaa agggccagtg actgctcaaa ttcaaaaagc atttttcggt
     3841 ctatttgatg gttctacaaa agattcttgg gactggttag aaccgatcca ataagtcatc
     3901 catggctaaa acattatttg ataaaatctg ggaacagcac gtggttgttc cagaaacgac
     3961 caatactcca gcaatactct atattgatct acatttaatt catgaagtaa cctcccctca
     4021 gggctttaca ttactcaggg agaataatct gcagatgaga gctcctcaaa gaattcttgc
     4081 gaccatggat cactctacac caacagttcc tattcactcg ttaaaagatc tcgatatcgt
     4141 gtctgctaaa ggacctgcag atcaaattag agaaatgatt aagaactgtg aggattttgg
     4201 tatcgactta tttggattag acagtgaaca aagaggtatc gtccatgtca ttggaccaga
     4261 attgggagct acccaacctg ggaaaactat agtttgtggc gatagccata ccagtactca
     4321 cggggcattg ggtgctttag cttttggcat tgggtcaacc gaagttggtc atgtatttgc
     4381 aacccagtgc ttactccaga gaaagccaaa aacaatggca gtacatctaa atggtaagtt
     4441 aaatatgggg gtttcagcaa aagatatcat tttaaaggtc attaacagta ttggagtgga
     4501 tggaggtact gggtgtgtga ttgaatattt aggaagtgca gttccaagtt tatctcttga
     4561 agaacgaatg actatttgca atatgaccat tgaaggtggg gctagagctg gattatttgc
     4621 acccgatgat gcgacctttg attatttata tggcaagcct atgagtccaa aaggcaatga
     4681 ttgggatcaa gctgtaacac gatggaatga tttacaaaca gatgatgatg cagtctttga
     4741 taaggtgatt aatatcgatg ttgactcatt aacaccaatg attacttttg gaaccaatcc
     4801 tggcatggcg atgcctgttg atggagccat tccagatcat aatggcaaca aaagtgttaa
     4861 acaggcacta gattatatga attttgagtc gggtcagtca ttaatcaatc atccaataaa
     4921 tacagttttt attggcagtt gtaccaattc aaggatctct gacctacgtg atgcagcagc
     4981 tgtacttaaa ggaagaaaag tttctgagaa cgtcagagta ttaattgcac caggttcaca
     5041 aaaggtcaaa gctgctgcag aggctgaagg tttagataaa attttccaag aagccggagc
     5101 tgattggaga gagtctggtt gttcaatgtg cattggtatc aatggtgatc atcttgagaa
     5161 aggagagcta gctgttagca cgagcaatag gaactttgaa ggacgacaag gaccaggatc
     5221 cagaaccatc ttagcgagcc caaaaacagc tgcagcttct gcaatagtag gttcagttca
     5281 ggatccaagg caactcggtg ttattaatga gtaaaataat taaattttca gctcgaacgg
     5341 ttaatttgcc taacgaggat attgacacag accaaattat tcctgcaaga tttttagccc
     5401 aagcatccac ctttgaagac cttggtaaag atttatttgc tgattggaga tatgaaaaaa
     5461 caggagagtt aaaacaagat tttattctca accagcctga agcatcggag gcagaagttt
     5521 tggttaccgg aaataatttt ggttgtgggt catcaagaga acacgcacca tgggctttgg
     5581 tagattttgg ttttaaggcg attatcagca catcttttgc agatatcttt cgaaataatg
     5641 ccttgaagaa tggtttgatc ccaattgttg ttgacaggga aacacatcat tggttattga
     5701 atcatccggc tacaatggtc acaattgatt tggaagcaat ggaattatca ttagaaaatg
     5761 gacatgcagc tccctttcct attgagccgt tttcaagata ttgtttgatg agtgacatta
     5821 atcaactgga ctacatactg cgtcataatg cagaaataac tgcgtacgaa aataaagaat
     5881 aatgaaagca agcattacaa ttatgcccgg tgatggcgtt ggtccggaaa ttactcgtga
     5941 ggctgttaaa gtcctgcaag ccgttgaaac tatctttgat catgattttt tactcaaaga
     6001 ggttttattt ggcggcatag caattgatga gaccggatca ccctaccctg aggaaacaca
     6061 aaggtcttgt aaaagttctg atgcagtatt acttggcgct gttggcggac ctaagtggag
     6121 tgatcccaat atgaaagtca gaccggaaaa agaaggctta ttggaaatga gagctcattt
     6181 aggagtttat gcaaacataa ggccaattaa gacttatcct cagttgattg aaaactcacc
     6241 gattaaaaac cgttacttgg acaatataga tatggttttt gtcagagaac tgaccggtgg
     6301 gatttatttt ggtaagaagg aaaaaactga atcatccgct agtgatattt gtgaatactc
     6361 aaaaacagaa atcgaaagaa taacaagagt ggctgcaaat atagctcaag atagaagaaa
     6421 aaaaatcaca tctgtagata aagcaaacgt catggccacc tcccaactgt ggagaagtac
     6481 tacaactgaa ttaatagcag aggaatatac tgatcttgaa cttgaacaca tttttattga
     6541 tgcagcagca atgcatttat tgtcaagacc cgccgatttc gacgttatat taactgataa
     6601 tctttttggt gacattctaa ctgatgaggc agccatatta actggttcca ttggtatgtt
     6661 gccttctgct tgcttgggtg aaaatagttt tggtgttttt gaacccatcc atgggtcagc
     6721 tcctgatata gcgaatcaag ggattgccaa tccctgtggt gccatattat ctgctgcaat
     6781 gttgttgcaa tactcgctta acctgaaaga agaagcacaa gttatagaac aatcagtttc
     6841 tggggttatt gaggatggta tattgaccaa agatttaact gagagtagct atgtttctac
     6901 tgaggaagtg ggaacagcag tgatggaaaa gatactccag acttaggaca gtaaatcaag
     6961 caaccctaaa tttagttaga gttgcctgat aaacgacata aagatcattc cgcagaataa
     7021 atctgttcac acaattcata ggtatcgctt acgacttcat caatcattgt tcttgatttt
     7081 tcccatatgc gtccccaaga agcgcctgca aagtattcat cagcaatttg acctgattct
     7141 ct
//
LOCUS       DASCTH010000084         4134 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000120931, whole genome shotgun sequence.
ACCESSION   DASCTH010000084 DASCTH010000000
VERSION     DASCTH010000084.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000120931"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(140..215)
                     /locus_tag="QGH35_01810"
     tRNA            complement(140..215)
                     /locus_tag="QGH35_01810"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Asn,seq:gtt)
     gene            complement(270..1475)
                     /locus_tag="QGH35_01815"
     CDS             complement(270..1475)
                     /locus_tag="QGH35_01815"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010506548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HJL81302.1"
                     /translation="MIQKQKKQNNSSITRRINRIQPSATLTISAKAMELRAQGLDIIS
                     LSQGEPDFDTPEHIKKGVIQAINRGATKYTAVDGTPEFKEAIINKFSKDNHLIYQPEN
                     ILVSSGAKQTCYNLFQSVLEEGTEAIVVSPYWVSYPDMVILTGATPVILETTPQNNFK
                     LNTVDLRSLLNDQTRLLILNSPSNPTGFTYSKDQYQEIGEVLKDFPNVWIASDEIYEH
                     IYWGREKYYSFADACPDLFHRTITINGVSKAYAMTGWRIGYCGGPVEIIQAMKKIQGQ
                     STSNASSISQAASVSALNGSLDCVISMVREYKNRYHYLFNTLEKINGFKLQESSGAFY
                     TFPEVSGAINHLNLSDDIEFSKYLLEKAQVAVIPGSAFGAPGHIRISFATSMELLEEA
                     MDRIKRVVE"
     gene            1507..3543
                     /gene="uvrB"
                     /locus_tag="QGH35_01820"
     CDS             1507..3543
                     /gene="uvrB"
                     /locus_tag="QGH35_01820"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HJL81303.1"
                     /translation="MSEEYFKLVANYKPKGDQPNAIAGLIDGLNSGLSKQTLLGVTGS
                     GKTFTIANLINIQQRPTLILAPNKTLAAQLYGEMREYFPENKVEYFVSYYDYYQPEAY
                     VPSTDIYIEKDASVNAHIEQMRLSATKALIERQDTIIVATVSAIYGLGDPELYLSMVL
                     HLSIKDKIDQRAVLRRLAELQYQRNNIDFKQGTFRVLGDVIDIYPADSEKYAVRIELF
                     DNEIDGLSYFDPLTGELDRRVRQLTVFPKTHYVTPREVMLNATKKIKKELVIRLKELN
                     KHNKLVEAQRLEQRTMYDIEMIKELGFCSGIENYSRYLSGRKAGEPPPCLMDYLPNNA
                     LVVIDESHVTIPQLGGMYKGDHSRKCTLVEYGFRLPSALDNRPLMFEEFESVTPQIIY
                     VSATPREYEMKQSDAIIELVVRPTGLVDPVVEIRPVNNQVDDLLSEIRIRTSNKERVL
                     VTTLTKRMSEDLADYFNDQDVKVRYLHSDIDTVERSEILRDLRLGVFDVLVGINLLRE
                     GLDLPEVSLVAILDADKEGFLRSEGSLIQTMGRAARNINGKAIMYADRITGSMHRAIK
                     ETNRRRERQIQFNKDHKITPKTIVKNVQDVMDGARSRTIKERLKIPKSIQKTFDPKHP
                     EKLSKTIESLEIKMFEAAKNLEFEEAAKLRDEITLIKEFTFGVAYVHDQNLRAN"
     gene            3613..3912
                     /locus_tag="QGH35_01825"
     CDS             3613..3912
                     /locus_tag="QGH35_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81304.1"
                     /translation="MRIKLYIILGLLWCFTFHSVAVHANNHKIKGSYEYGEKIAKRSY
                     GSGCFGGCHSHSIDMFKGSSPEAVLERLEASWHIASLTDEDLRDVSVFLSIKANQ"
     gene            complement(3936..4007)
                     /locus_tag="QGH35_01830"
     tRNA            complement(3936..4007)
                     /locus_tag="QGH35_01830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3973..3975),aa:Val,seq:gac)
BASE COUNT         1344 a          714 c          796 g         1280 t
ORIGIN      
        1 aatcagtgtg ggtatcaaac tatcaaatta acatggcaat gccccacttg tagacattgg
       61 gaaacagcta cctcaataga ctttgtccat agtaatgatt gaatcgtaat tgatatatag
      121 cctgtgggaa atctagctct ggctccccgg gccggactcg aaccagcgac caattgatta
      181 acagtcaatc gctctaccaa ctgagctacc ggggagtaga gttagttcga agtctgcttt
      241 aactacatcc gatagtcaag gagaagaaat tactcaacca ctcgcttaat tctatccatg
      301 gcttcttcga gtaattccat gctcgtagca aaggatatcc taatatggcc tggtgctcca
      361 aaagcagaac ctggtataac cgctacctgt gctttctcaa gaaggtattt agagaattct
      421 atatcatccg ataaatttaa atggttaatg gcacctgata cttcaggaaa agtataaaat
      481 gcaccagagc tttcttgtag tttaaaacca tttatttttt caagagtgtt gaataaataa
      541 tggtacctgt tcttatattc tcgaaccata gatataacac agtcaagtga tccgttcagt
      601 gctgaaactg aagctgcttg cgatatcgat gatgcatttg atgtactttg accttgaatc
      661 tttttcatag cttggatgat ttctacaggg cctccacaat agccaattct ccagccagtc
      721 attgcatagg ctttagaaac accatttata gtgattgttc tatgaaacaa atctggacag
      781 gcatcagcaa aactataata tttctctcta ccccagtaaa tatgttcata tatttcatcg
      841 gatgcaatcc atacgtttgg gaaatctttc aatacttctc cgatctcttg atattggtct
      901 tttgagtatg tgaaccctgt tggattggaa ggtgaattga gtatcagtag cctggtttga
      961 tcattcagaa gggatctcag atcaactgta ttaagtttaa aattgttctg tggagttgtt
     1021 tctaaaatga ctggagttgc ccctgtaagg atgaccatat ctggatacga aacccaatat
     1081 ggactgacca cgatcgcctc tgtaccttct tcaagaactg attgaaataa gttataacaa
     1141 gtctgtttag caccagatga aacgaggata ttttctggtt gatatattaa atgattatct
     1201 tttgagaatt tattaattat cgcttcttta aactctggtg ttccatcaac tgctgtgtat
     1261 ttggttgcgc ctctgtttat tgcttgtatc acacctttct taatatgttc gggagtatca
     1321 aaatcaggct ctccttgact taaacttata atatcaagac cttgtgctct aagctccatt
     1381 gctttggctg aaatggttag ggttgctgat ggctgaatac gattgatacg cctagttatg
     1441 gatgaattat tctgtttttt ttgtttttgg atcataaaga tttttacaac gttataatat
     1501 caaattatgt cagaggaata cttcaaatta gttgctaact ataaaccaaa aggtgatcag
     1561 cccaatgcca ttgcaggact tattgatggt ctgaattctg gtttatcgaa acaaacactt
     1621 ttgggtgtaa caggatctgg taaaacattc acaattgcaa atttaattaa tatccagcaa
     1681 agaccaactc tgattcttgc ccccaataaa actcttgcag cacaattata cggggaaatg
     1741 agagaatatt ttccagagaa taaagttgag tattttgttt cttattatga ttactatcaa
     1801 cctgaagcat atgttccttc tactgatatt tatattgaga aagatgcatc cgttaacgct
     1861 catatagaac agatgagact atctgcaaca aaagcactga tcgaacgaca agatacaatt
     1921 attgttgcta ccgtctctgc aatttatgga ttaggggatc cagaactgta tttaagtatg
     1981 gttctgcatt tgtccatcaa agataaaatc gatcagagag ctgtgctaag gagattagca
     2041 gagcttcaat accaaagaaa taatattgat ttcaaacaag ggacatttag agttttgggt
     2101 gatgtaattg atatttatcc tgcggattct gaaaaatatg cggtcagaat tgagttattt
     2161 gataatgaaa tagatggtct ttcttacttt gatccgttaa caggagaatt agatagacga
     2221 gttcgtcaat taactgtgtt tccgaaaaca cattatgtga ctccaagaga agtcatgctc
     2281 aatgcaacaa agaagataaa aaaagaactt gtcatccgtc tcaaagaact gaataaacat
     2341 aacaagttag tcgaagctca aaggcttgaa caaagaacca tgtacgacat agaaatgatc
     2401 aaagagttgg gattttgttc aggaatagaa aattacagta gatatctttc gggaagaaaa
     2461 gctggagagc ctcccccttg tttaatggat tatctcccta ataatgcttt agtggtaata
     2521 gacgaaagtc atgtaacaat tcctcaattg ggtggaatgt ataaaggaga tcattccaga
     2581 aaatgtacac ttgtagaata tggatttaga cttccttctg ctctggataa tcgaccacta
     2641 atgtttgaag agtttgagtc agtcacacca caaataatct atgtctcagc aactcctaga
     2701 gaatatgaaa tgaagcagtc tgatgccatc atagaattag tggtacgacc cacaggtctt
     2761 gtagacccgg tagtcgaaat aagaccagtc aataaccagg tggatgatct tctttcagaa
     2821 ataagaataa gaacgagtaa taaagaaaga gtcttagtta caacattaac taagagaatg
     2881 tcagaagatc ttgctgatta ttttaatgat caggatgtaa aagttcgata cctacactct
     2941 gatatcgaca cagtggagcg ttctgagata ttaagagatt taagattagg agtttttgat
     3001 gttctagttg gaatcaatct tctcagagaa ggacttgatc tccctgaagt ttcattggtt
     3061 gctatcttgg atgctgataa agaggggttt ttgaggtctg aagggtcttt aattcaaact
     3121 atgggaaggg cggcaagaaa tatcaatggc aaagctatta tgtatgcaga ccgaataact
     3181 ggatccatgc atcgtgccat taaagaaacc aatagacgaa gggaacgaca gattcaattc
     3241 aacaaagatc ataaaattac accaaaaaca attgtaaaaa atgttcaaga tgttatggat
     3301 ggagcacgtt ctaggactat aaaagaacga ttaaaaatac ctaaatcaat tcaaaaaacc
     3361 tttgatccaa aacatcctga aaaactcagc aaaacaattg agagtttaga aattaagatg
     3421 tttgaagctg caaaaaatct tgaatttgag gaagccgcta aactcagaga tgaaatcacg
     3481 ctcatcaaag aattcacctt tggggttgct tatgttcatg atcaaaatct aagagctaat
     3541 taaataggat gcccattggc ttgtttttat gttttatata tgatttattg actttatgag
     3601 tagagtagaa aaatgagaat aaaactatac atcatcctgg gtctgttatg gtgctttacc
     3661 tttcattcag ttgctgttca tgcaaacaat cataaaatta aaggcagtta tgaatatgga
     3721 gaaaaaattg ctaaaagatc ttacgggagt ggttgttttg gaggatgcca ttctcattca
     3781 attgatatgt ttaaaggctc aagtcctgag gcagttttgg aaaggctaga agcatcttgg
     3841 catatcgcaa gccttacaga tgaagatctt agagatgtct ctgtatttct ttctatcaaa
     3901 gccaaccaat gattgataat ggaggagggg aagtctgggc gttgtaagat tcgaacttac
     3961 gaccttctcg atgtcaccga gacattctac cactgaacta aacgccccag acgttgaaac
     4021 gtattatcac tttttatctt tttaatatct actcaataga tttcttttac ctttatataa
     4081 ctacgcttga accttaaatt atttcctggg ctctattagg attaaatatt catt
//
LOCUS       DASCTH010000085         6249 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000122786, whole genome shotgun sequence.
ACCESSION   DASCTH010000085 DASCTH010000000
VERSION     DASCTH010000085.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000122786"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="QGH35_01835"
     CDS             complement(<1..1527)
                     /locus_tag="QGH35_01835"
                     /inference="COORDINATES: protein motif:HMM:NF039586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HJL81305.1"
                     /translation="MATFQIKQKITKRIIFLLLGLSAFSLLALMFASDNPEEFNRLQT
                     PILMVNTVSFFVLFVLVAVSGRQLVNDYKKNKLGAKLRLKMTLVFGMLSVIPIAVVFF
                     FATNFMNKGIDVWFDAEVETGLTNALMLGRSALDEQIQEGLFKSTNMSLKLTSIEEID
                     QQLHTLRESSLALELLVLNRELQIIGQSTQTLSGLNPRLPTKAEIIQVDSKQSWTNLD
                     ASSEGAYLIRVLVPILGLSSNEIPLYLQSIFSVDPALSMMAESVEQTYAKYGRLTFLR
                     IPLQYSYMFTLALVVLLALLLTIYGSIEFSKSLVEPIESLEEGTRSVAEGDLDIHIPR
                     AGKDEIGILVNSFNEMVKKLASAQTEANESRKIIEHERATLQAILSGLSAGVIVLDER
                     QNISLINPAASVILNVDTEQYTGCNVKIIIKTNDLFSQFISKTMSKIEHNKTWREKIT
                     LDLADSTREIMCSYKELSSSTNSAMLLLVFEDISDFLKAQQEAAWSDVAKHMAHEIKN
                     P"
     gene            complement(1509..2081)
                     /locus_tag="QGH35_01840"
     CDS             complement(1509..2081)
                     /locus_tag="QGH35_01840"
                     /inference="COORDINATES: protein motif:HMM:NF025692.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HJL81306.1"
                     /translation="MKNNINKIKGYMVVALVVFLFTTSIVYAQPVKLIKGESFLIEGV
                     YYSDINIEFSFDRAYLQALNSGLVFDIDLDFLIVNIKPWRVDQEIGQLSQNYTIKYNA
                     FTQRYTVLNTNTGRETSYPTIETTLSNLGTINKFPVLDDSLINIDESYQVFLSASIKA
                     QGVPQWIKTLTFWRDSLDFQTEQIQWPLFK"
     gene            complement(2158..3105)
                     /gene="fmt"
                     /locus_tag="QGH35_01845"
     CDS             complement(2158..3105)
                     /gene="fmt"
                     /locus_tag="QGH35_01845"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HJL81307.1"
                     /translation="MKAASIKIIIAGASAFSVPTIKRLNNLDYQIVAVMTQPDRAAGR
                     GLKQTKNPVKQFAEKVGLTLLDFESLDRENVFATIKNLSPDLLITIAYAGLIPQKILD
                     IPHCESINVHPSLLPLWRGASPIQSSILNGDTKTGISLIRMTSELDAGPVFAVAETFI
                     DPEENALALSSRLANMSARLITDNIVEIVNKNLLPNEQKHQKATYAPKLTKQDAIINW
                     TRPAPEILKKIKAFNPWPIAHSILEQKNFRIWDAKLSKQTNDTLKPGSIIKTSSNEIL
                     VTTGDGMLSLISVQLEGRKKMPALDFCKGFAYEGKTLGQ"
     gene            complement(3122..3655)
                     /gene="def"
                     /locus_tag="QGH35_01850"
     CDS             complement(3122..3655)
                     /gene="def"
                     /locus_tag="QGH35_01850"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HJL81308.1"
                     /translation="MKTLKVITEPDKRLRKKSKPVHDFDDQLIRFSKDMLATMYKEQG
                     IGLAAPQVNKPIRLIVLDISENRDEPLVFVNPVIDSHQGSVDSNEGCLSVPDIKADIK
                     RFESIMFRAESIRGKKISFAADGLLSVCIQHEIDHLDGKLFIDQISDVKLQRIRKKIK
                     KQKKAAAIEKQNNARII"
     gene            3840..4316
                     /locus_tag="QGH35_01855"
     CDS             3840..4316
                     /locus_tag="QGH35_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016264.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HJL81309.1"
                     /translation="MKEAFLDVLLYLFEYYSEEPGLSKSNETQIRNELVLAGFKKDAI
                     EHAMDWVDVFNKSPNKINIKKPKKYSVRILSDSEKSLINEECQNYITRLERFGLLPPL
                     KREVLIDKLTSIGFEPLDIEVVKALSILMLFQEPNIDVKLNTIEGDAFWESPSKLN"
     gene            4379..>6249
                     /gene="topA"
                     /locus_tag="QGH35_01860"
     CDS             4379..>6249
                     /gene="topA"
                     /locus_tag="QGH35_01860"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HJL81310.1"
                     /translation="MTKLVIVESPAKAKTIQQYLGGDYRVLATIGHIRELPKKDAIDP
                     ANNYAMTYITIPGKEEAIKKITDALKDVEEVILATDPDREGEAIAWHVSELLKTKHAK
                     KINGKEIKRAVFYEISKEAVKDAVSKPRDIALDLVESQETRRALDRHFGFTLSPLLWR
                     LFPSNNHSAGRVQSPALRMIVEREKEIEAFVPQEYWTVGALLQKEEKIDVKLTLYENE
                     PVKKFTFVSSALVESATTKLETGCKNGMRVASVVNKQIKRGPKAPFRTSVLMQQASAK
                     FSFTPKDTMRYAQSLFARGDKGDGLITYIRTDSIEIDKTKLPLIQQKVVALYGEDFLE
                     RRKFKNKKEAKNTQEAHGAITPVDINILPDSLKTQLSDNEFKLYNLIWERTIASQMKD
                     AIFERTTIEFVPNGLPDLASFVFSEQKLLFPGYLLATKEEVRNKKPPNIKEGDVVDFL
                     NLTKEQHFTEPPPRFNDASMIKALEEKGIGRPSTYAGILSRLLDRAYIISTQRRYEPS
                     DMGRLVSNFLNGSFADYINDEFTSKMENNLDAISNGSKSKKDVLDQFWSPLEEEVATA
                     GKKLTRRDVNPQRLLGTDPEVNKPIFARMTKNGPAVQRGDIDAGDDPEWRALKKGQKL
                     "
BASE COUNT         1973 a         1160 c         1206 g         1910 t
ORIGIN      
        1 tgggttttta atttcatgtg ccatgtgctt tgcaacatca ctccaagcag cctcttgctg
       61 tgcttttaga aaatcactga tgtcctcaaa aaccagcaat aacatagcac tatttgttga
      121 gctagataat tctttataac tacacataat ctctctagtg ctatctgcta aatcaagtgt
      181 aattttttct ctccaggttt tattgtgttc tattttagac atagtcttag agataaactg
      241 gctaaataag tcattagttt taataataat tttcacattg cagcctgtat attgttcggt
      301 atcaacgttc aaaatcacag aagccgctgg gttaattagg ctaatatttt gtctttcatc
      361 caacacgatg actccagcag acaagccgga aagtatagcc tgtagtgtgg ctctttcgtg
      421 ttcaataatt tttctactct cgttggcttc tgtttgagcg cttgcaagtt ttttgaccat
      481 ctcattaaaa gaattcacca atataccaat ttcatctttt ccggctcttg gtatatgaat
      541 gtctaaatca ccctctgcaa ctgaccttgt tccttcttcc agtgattcta tgggttctac
      601 taaactctta gaaaattcga tggagccgta tattgtaagc aacaatgcca acaaaacaac
      661 taaggctaga gtaaacatat aactatattg aagaggtatt ctaagaaagg ttagacgtcc
      721 gtattttgcg taggtttgct caactgattc agccatcatg ctcaacgctg ggtccacgct
      781 aaatatactc tggagatata atggtatctc gtttgaagaa agccccaata taggcaccaa
      841 tacccggatt aaatatgccc cttcagaaga agcatcaaga tttgtccatg attgtttact
      901 gtctacttgg ataatttctg ccttggtagg aagcctaggg tttaaaccgc ttaatgtttg
      961 agtgctttgt cctataatct gtagctctct atttaagacc aacagctcta aagccaaaga
     1021 gctctctcta agtgtatgaa gttgttggtc tatctcctca atacttgtga gcttgagtga
     1081 catatttgtt gatttaaata atccttcctg aatttgctca tcgagcgcag agcgccctag
     1141 cataagcgca ttagtaagcc ctgtttctac ttctgcatca aaccaaacat caattccttt
     1201 attcataaaa tttgttgcaa agaaaaaaac tactgcgatt ggaataacag agagcatccc
     1261 aaaaaccagg gtcattttta aacgtagttt ggcgcccagt ttgttttttt tataatcgtt
     1321 aaccagttgc ctgccgctca ctgctacaag cacaaacaac acaaagaatg agaccgtgtt
     1381 gaccattaaa ataggtgttt gcaatctatt aaattcttcc gggttgtcag atgcaaacat
     1441 aagagccaat aaagaaaagg cacttaatcc tagcagtaaa aaaatgattc tttttgttat
     1501 tttttgcttt atttgaaaag tggccattgg atttgttctg tttgaaaatc taaactatcg
     1561 cgccaaaagg ttagggtttt aatccactgt gggacccctt gggctttaat gctagccgat
     1621 aaaaaaacct gatagctctc atcaatatta atcaatgaat cgtccaacac aggaaattta
     1681 ttgatggtgc ccagattact cagggttgtt tcaatggttg ggtaagatgt ttctcttccg
     1741 gtgttggtgt ttaaaacagt gtacctctga gtaaaggcgt tgtatttgat ggtgtagttt
     1801 tgactcagtt gaccaatttc ttgatcaacc cgccaaggtt taatattgac aatgagaaaa
     1861 tcaaggtcaa tatcaaaaac caaaccagag tttaaagctt gaaggtaggc tctatcaaaa
     1921 gaaaattcta tattgatatc agaataataa accccttcta ttaaaaaaga ttcgcctttt
     1981 attagtttaa cgggctgtgc ataaacgatg cttgtggtaa atagaaaaac cactaacgcc
     2041 acaaccatat atcctttgat tttattaatg ttgtttttca tatcgggtta aatagtttat
     2101 tagaaaacca tctattatta ccagtatgtg tttaccaaga acacaggatt tgctagatca
     2161 ctggcctaga gtttttcctt cataagcaaa gcctttgcaa aaatccagtg ccggcatttt
     2221 ttttctacct tctagttgca cgctgattaa ggaaagcatt ccatcacctg tggtaactag
     2281 tatttcgttt gatgaagtct tgatgataga accaggctta agggtatcat tggtttgctt
     2341 gcttagttta gcgtcccaaa tacgaaagtt cttttgttct agtatagagt gcgcaattgg
     2401 ccaagggttg aatgctttga tttttttcaa aatctctggc gctggtcttg tccaattaat
     2461 aattgcgtct tgttttgtaa gttttggtgc atacgttgct ttttggtgtt tttgttcatt
     2521 aggaagtaaa tttttattaa ctatctctac aatattatct gtgattaacc ttgcagacat
     2581 attcgccaga cgacttgata aagccagcgc gttttcttct gggtctataa atgtttcagc
     2641 tacagcaaaa acaggaccag catcaagctc agaagtcatt ctaattaaac tgattcctgt
     2701 tttggtatcg ccgtttaaaa tagacgactg gataggggac gcacctcgcc ataagggcaa
     2761 caaagagggg tggacattta ttgattcgca atgtggaata tctagaattt tttgtgggat
     2821 taaaccagcg taagcaattg ttattaagag atcaggggac aggtttttaa ttgttgcaaa
     2881 aacattttct ctatctaatg actcaaaatc tagtagtgtt aatcctacct tttcagcaaa
     2941 ttgtttaact gggttcttgg tttgttttaa ccctcttcca gctgccctat caggttgggt
     3001 cataacggcg acaatttggt agtctaaatt attaagtctt ttaattgtcg gcacagaaaa
     3061 agctgatgcc cctgcaataa taattttaat agaggccgct ttcattcaca caatccctca
     3121 tttatattat tctcgcattg ttttgctttt ctattgcagc tgcttttttt tgttttttta
     3181 ttttttttct aatcctttgt agttttacat cagaaatttg atcgataaaa agcttgccgt
     3241 ctagatggtc tatttcgtgc tggatacaca cggacaaaag cccatcagcg gcaaaagaga
     3301 ttttctttcc acgtatagat tccgccctaa acataatact ttcaaagcgt ttgatgtctg
     3361 ctttgatatc tggaacagaa agacagccct cgttgctatc tacgctccct tgatgtgaat
     3421 cgataacagg attaacaaaa actaatggct cgtccctatt ttcagagata tctaaaacaa
     3481 tgagtcgaat gggtttgtta acttgcggag ccgcaagacc aatcccttgt tctttataca
     3541 tggtggccaa catatcttta gaaaatcgta ttaactggtc atcaaaatca tggacaggtt
     3601 ttgatttttt tctgagcctt ttgtctggct ctgtaattac ctttagtgtc ttcataacca
     3661 caacatacca ctgaaaatta ataaaagagt cacatgatag cacttagaag actagtctta
     3721 tcagctcttg agagcaatcg ttagaaaaga ataaaaaaat aaaaatgttt gacctattgc
     3781 ttcttttcgt acattctcaa cgaaaccaac cactatttcc ttgttatatg aatcataaaa
     3841 tgaaagaggc cttcctggat gtgctgcttt atttgtttga gtattattca gaagagcctg
     3901 gtttaagcaa aagcaacgaa acccagatta gaaacgagct tgtactggct ggttttaaaa
     3961 aagatgctat tgagcacgcg atggactggg ttgatgtgtt taacaaatca cccaacaaga
     4021 ttaatattaa aaaaccaaaa aaatactcag ttagaatttt aagcgacagt gaaaaatctt
     4081 tgattaatga agagtgtcaa aactacatta caagacttga aagatttggg ctgttaccac
     4141 ccttaaagag agaagtgctc attgataaac tgacaagtat tggttttgag cctttggata
     4201 ttgaggtggt aaaagccctg agtattttaa tgcttttcca agaaccaaat attgacgtga
     4261 aactaaatac catagaggga gacgcttttt gggaatcccc ctctaaactg aactaaataa
     4321 aaaaacaatg gactatatta tagccgcgca cgtcgcggct tgtttataga ataataaaat
     4381 gacaaagctt gttatcgttg agtcgcccgc taaagcaaag accatacaac aatacttggg
     4441 tggtgattat agagttttag ccactattgg gcatattagg gaattaccaa aaaaagatgc
     4501 tattgaccca gcaaacaatt atgcaatgac ttacatcacc atccctggaa aagaagaagc
     4561 cattaaaaaa ataactgacg cactaaaaga tgtagaggaa gttattctgg ctactgaccc
     4621 tgacagagaa ggtgaggcca tcgcctggca tgtctctgag cttttaaaaa caaaacacgc
     4681 taaaaaaatt aatggaaaag aaattaaaag agctgtgttt tatgaaatca gtaaagaggc
     4741 ggtcaaggat gccgtttcta aacctcggga catagccctg gatttggttg aatcacaaga
     4801 aacaagaagg gctttggata gacattttgg gtttacccta tcccctcttt tgtggcgact
     4861 ttttccaagc aacaatcact ctgcaggaag agtgcaaagt ccggcattgc ggatgattgt
     4921 tgaaagggaa aaagaaattg aagcgtttgt gcctcaagaa tattggacgg ttggcgccct
     4981 gttgcaaaaa gaagaaaaaa tagatgtaaa actaaccttg tacgaaaacg agcccgtaaa
     5041 aaaattcact tttgtgtcca gcgctcttgt tgagagcgct accacaaaac tggaaactgg
     5101 ttgcaaaaat ggcatgagag tagcttctgt tgtgaacaag caaataaagc gtggtccaaa
     5161 agcaccgttt agaacctcgg tgctaatgca acaagcatca gctaaattta gttttactcc
     5221 taaagatact atgagatatg cacaatcact gtttgcaagg ggagacaagg gtgatggatt
     5281 gattacatat ataaggactg actctattga aattgataaa actaaactgc ctttaattca
     5341 acaaaaagtt gttgctcttt acggagaaga ttttcttgaa agaagaaagt ttaaaaacaa
     5401 aaaggaggca aaaaatactc aagaagctca tggagctata acgccggttg atataaatat
     5461 attaccagac agcttaaaaa cacaactgag tgacaatgag tttaagcttt ataatttaat
     5521 ttgggaaaga actatagctt cccaaatgaa ggatgctatt tttgaacgaa cgactatcga
     5581 atttgtgccc aacggtttgc ccgacttagc ttcctttgtt ttttctgaac aaaaactgct
     5641 ctttccaggt tatcttctag caacaaaaga agaggtgcgt aacaaaaaac cgcccaatat
     5701 aaaggaaggg gatgttgttg attttctaaa cctgaccaaa gagcagcatt ttacggagcc
     5761 accaccaagg tttaatgatg caagcatgat aaaagccctt gaggaaaaag gcattggcag
     5821 gccctcaacc tatgcgggta tcttatctag gttgctagac agggcctata taatctcaac
     5881 ccaaagaagg tatgagcctt ctgatatggg cagactcgtt agcaacttct tgaatggaag
     5941 ctttgctgat tacatcaatg acgagttcac ttcaaaaatg gagaataatt tagatgctat
     6001 atctaatggt tccaaaagca aaaaagacgt cttggatcaa ttttggtcgc ctttagaaga
     6061 agaggtggca acagcaggaa aaaaattaac aaggagagac gtgaacccac aaagattgct
     6121 tggcacagac ccagaagtta acaagcctat ttttgctcgg atgacaaaaa acggccccgc
     6181 tgttcagagg ggggatatag atgccggtga tgatcccgaa tggagagctc ttaaaaaagg
     6241 acaaaagct
//
LOCUS       DASCTH010000086         1727 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000123282, whole genome shotgun sequence.
ACCESSION   DASCTH010000086 DASCTH010000000
VERSION     DASCTH010000086.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000123282"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="QGH35_01865"
     CDS             <1..402
                     /locus_tag="QGH35_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HJL81311.1"
                     /translation="LMGLLQGAFLAQYEVLREHGHSPSEAYNETIEEALQSLYPLVAE
                     NGMDWMYANCSTTAQRGALDWAPKFKDTLKPIIEECYQNVLSGEEARISIKTNSKADY
                     REQLEKELREINDQEMWQAGKQLRPLRPENI"
     gene            419..1528
                     /gene="leuB"
                     /locus_tag="QGH35_01870"
     CDS             419..1528
                     /gene="leuB"
                     /locus_tag="QGH35_01870"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HJL81312.1"
                     /translation="MKSYHLTLLPGDGIGPEIMDAAIKVLDLVGQQFNVQFDYEEALF
                     GGCSIDRNGVPLTDETIEQCRKSDAVVMGAVGGPIWDTQPHENKPEQGLLKLRASLGL
                     YANLRPATIYNALLDASSLKPTVIKNADIMVVRELTGGIYFGTPRGMDKGKGWNTLSY
                     TKEEVERIARTAFDLAQKRNNHVTSVHKANVLESSQFWREIVEEVHQDYSDVVLDEMY
                     VDNAAMQLVRDPKQFDVILTQNLFGDILSDISAMITGSLGMLPSASLGEEYAVYEPVH
                     GTAPEIAGQNKANPLAMIGSIAMMLDYTFGMPEAATMIYDAIGEVLEMGSRTEDIATP
                     NTNVVSTTEMRDLVLERCEGKSKTIIEMVRMSYEG"
     gene            1518..>1727
                     /locus_tag="QGH35_01875"
     CDS             1518..>1727
                     /locus_tag="QGH35_01875"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854916.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HJL81313.1"
                     /translation="MKDKIVIFDTTLRDGEQAPGASLNMTEKIEIAKQLNRLNVDVIE
                     AGFPVSSKVQFDSVQRIADAVDTVVA"
BASE COUNT          559 a          283 c          413 g          472 t
ORIGIN      
        1 cttatggggc tattacaagg ggccttcttg gctcagtatg aagtccttcg agaacatggt
       61 cattctccat cagaagccta caatgaaact attgaagaag cacttcaaag tttatatcca
      121 ttggtggctg aaaatggaat ggattggatg tatgcgaatt gttcaacaac tgcccagcgt
      181 ggtgcattgg attgggcacc aaagtttaag gatacattaa agccgattat tgaggagtgt
      241 taccaaaatg ttttgagcgg agaggaagcg agaatttcaa taaaaaccaa ttctaaggca
      301 gattatcgcg aacaattaga aaaagaactg agagaaataa atgatcagga aatgtggcaa
      361 gctggcaaac agcttcgccc actgagacct gaaaatattt aaataaaagg cactcgttat
      421 gaagtcttat catttaacac tattgcccgg tgatggcatt ggacctgaaa taatggatgc
      481 agccataaag gtgttggatt tagtgggcca gcaattcaat gttcaatttg attatgaaga
      541 agcgttgttt ggtggatgtt ccatcgatag aaatggtgtt ccattgacag atgaaacaat
      601 tgaacaatgt cgtaaatctg atgcagtagt catgggtgca gtgggtggac caatttggga
      661 tactcaacca catgagaata aaccggaaca gggattatta aagttacgag cttcgctggg
      721 tttatatgca aatctgcgac cagctacaat ttacaatgcg ttgttagatg cctcttcatt
      781 aaagccgaca gtaattaaaa atgctgatat tatggtggtc agagaattaa ctggtggaat
      841 ttactttggc acgccgagag gaatggataa aggcaaaggg tggaatacct tatcttatac
      901 gaaagaagag gtggaaagaa ttgctcggac tgcatttgac ctagcccaaa aacgaaataa
      961 tcatgttaca tctgttcata aagccaacgt attagaaagt tcacaattct ggcgtgaaat
     1021 agtggaagaa gtccatcaag attattcgga tgttgtgttg gatgaaatgt atgtcgataa
     1081 tgctgctatg cagttggttc gggatccaaa acagtttgac gttattttaa cccagaattt
     1141 gtttggagat atattgagtg atatttctgc aatgattaca ggcagtctcg gcatgcttcc
     1201 ttctgcaagt ttgggtgaag aatatgccgt ttatgaaccg gtacatggca ccgcgccaga
     1261 aatcgcaggt caaaataaag caaatccact ggccatgatt gggtctatag ccatgatgct
     1321 tgattataca tttggaatgc cagaagcggc aaccatgatc tatgatgcca ttggtgaagt
     1381 acttgaaatg ggttcccgaa ctgaggacat tgcgacaccg aatacaaatg tggttagtac
     1441 aacagaaatg cgcgatttag ttttggaaag atgtgaaggc aaatcaaaaa caattataga
     1501 aatggtcagg atgtcgtatg aaggataaaa tagtcatatt tgataccact ctccgtgatg
     1561 gtgaacaagc accaggcgca tcattaaata tgacagaaaa aattgaaatt gctaaacaat
     1621 taaacagact gaatgtggat gttattgaag ccgggtttcc tgtttcatct aaagtacagt
     1681 ttgattcggt tcaacgaatt gcggatgcag tggatacagt tgtggcc
//
LOCUS       DASCTH010000087         7184 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000125868, whole genome shotgun sequence.
ACCESSION   DASCTH010000087 DASCTH010000000
VERSION     DASCTH010000087.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000125868"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            126..1001
                     /locus_tag="QGH35_01880"
     CDS             126..1001
                     /locus_tag="QGH35_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81314.1"
                     /translation="MNLGRISTVTIGSHDLDSMVEAYRDYLGYRLTKTGKITKLEATH
                     WQAKNITNSEYVILQPEKSDDFSFRFIQQPNQPDYIPFKSEGWNAAELIVQNVDKLSE
                     ELIDSPFEIIGPPADLSFNKNIRAMQVIGPAKEILYLTEFKKKIPEFDSPVPRCFVDK
                     TFIVILAGKSLESMQIYFHDDFMIAKAPIIESRIRSISRVFDLPEETQYPAAALTLKD
                     QCFIELDELPKEGISRVCIDGYLPSGIAMVSFLCYKESKYINNLYPSSLPGYKSNFCS
                     MEQGSVEELIELIYC"
     gene            1115..1936
                     /locus_tag="QGH35_01885"
     CDS             1115..1936
                     /locus_tag="QGH35_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014035.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HJL81315.1"
                     /translation="METIINNSLIGLVLLTLVAGGIAGILAGLLGVGGGIVIVPLLYY
                     ILTFLNYDQSIIMHVAVGTSLFVIVPTSIRSAMQHKKHDAFDARIFKKWFVPMVAGTI
                     IGSIIANYASFKALTGLFACIALIVSLQMFLNRQKINRNIKIPKFLYLIFPCFIGLIS
                     VMMGIGGGTMSVPVMNHLGVEMRKAVGTSASFGTVIAIPGTVGFIVGGMGNPLLPPLS
                     FGYINIMAFSLIVPATLLTVPIGVNYAHQISQLKLRKLFASFLGITAIKMLSDLF"
     gene            complement(1933..3663)
                     /locus_tag="QGH35_01890"
     CDS             complement(1933..3663)
                     /locus_tag="QGH35_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81316.1"
                     /translation="MKIKLILFAFLFIFLSTDYISNCSVISVVNAQDAEEKQARRTKR
                     VGSMSEKVAKKLGEAQELIEAERIEEGTEILNGILLQKRISNYERAYVYRFFGYVEYL
                     REDYKKAINYYNKILNEEAVPEGLQSEARFTIAQLWFQLEEWEKAISAVDALLATEES
                     PRTDLYELKGIALYQLKKYKESIPVIQSAINLAESRNAFRIEQLQKNINSAADDNGIR
                     YLRREEEEKNEIDYFRFATQIETELKQKLLKTRSSEKKEEIQQAIDSAKRSASNLAIG
                     PTKENWWLLLRAAYYELENIPKVREVLERLLIEWSKKEYWTQLSAIYGQDELEEKQIS
                     AYATAYQEGFLDKSSEYVQITQLYLSKEVPYKAAVILQTGIDEGIVDLEEKNWRLLSQ
                     AWFLAQEDQMAIIALREAAKLSDEGELDVRLARSLANIADYKSCVNAAKTGILKGGLK
                     RNDESYITLGMCQFEEAMYEDAKNSFNLATEDATARREAALEECSVRENMTQEDFLIL
                     FDRQARWEKIGKELENKKLVCPLPSSYKSATNWQKFLEKEVERVTLLQNQIKNIQEQL
                     KSKESSTITF"
     gene            complement(3665..4342)
                     /locus_tag="QGH35_01895"
     CDS             complement(3665..4342)
                     /locus_tag="QGH35_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010378710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HJL81317.1"
                     /translation="MADIAQRNKRLAGFRSISKFLEQIEDPRKRLALSVLFSLIITFA
                     ILWLMQILIATGKGAITSKYEGRFVDFVRIEKDESLETKTVKPKKPPEPEEPPPDTPP
                     ELDEIDTNTETLNVGAINANVGVGAEIGGFNAAEGEYLPIVKVAPVYPSRAQSRGIEG
                     YCIVEYIVTSTGTTRNPKIVECTSSLFQSASLKAALKFKYKPRVINGTPIDVPGVQTQ
                     ITFELEK"
     gene            complement(4348..4755)
                     /locus_tag="QGH35_01900"
     CDS             complement(4348..4755)
                     /locus_tag="QGH35_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008845615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HJL81318.1"
                     /translation="MRKNLFQAVQEEETEINLTPMLDVVFIMLIFFIVTASFVKESGL
                     DVNRPDAPTAVKKENANILVAIGPNNEIWIARRRIDPRAVRANIERLHAENPEGSVVI
                     QADKKAFHETVVLVMDSARQAGVFNVSIAAEPD"
     gene            complement(4781..5299)
                     /locus_tag="QGH35_01905"
     CDS             complement(4781..5299)
                     /locus_tag="QGH35_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016955212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HJL81319.1"
                     /translation="MIQDLIISVRDFLEMGGDVLSMIAFVIFFMWILILERLAFFQSG
                     LKIQKSDIQSTWNARAQKKTWESHAIREKLISQFNMSANQYMAIIKTLVAVCPLLGLM
                     GTVTGMIEVFDVMAISGSGNARSMASGVSKATIPTMAGMVGALSGVFLITVLNQKINK
                     EIEELEDNLTSE"
     gene            complement(5333..6700)
                     /locus_tag="QGH35_01910"
     CDS             complement(5333..6700)
                     /locus_tag="QGH35_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008845617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HJL81320.1"
                     /translation="MKNLLIITSLFLAFSFSIHAQQATNIDQLLDNVEQGKILDSADN
                     RKRENEFRQKRSQQEQLLDDSVKQKEAEEVKSATLEVIFEDNDKELNELQNTLNERLG
                     ALKELFGVMQQVSGDARSRFENSLTNIQYPDRGQFLEDLAKKIGSNSKLPSIPEIERL
                     WYELQREMTESGKVIKFSTDVIDVEGGKNKTEVIRVGSFNIVANGKYLNYEPETGNVI
                     EIPRQPEGRRFTGSTADLFNASGEKVAFGLDPTLGGVLMSLVARPNLIERIQQGGIVG
                     YLIILLGLFGVGIAIERLIRLVDADRKVTAQLKSDVVSKDNALGRVLSVYDENKNTDT
                     ETLELKMAEAVFKETPELNKGLILIKVISVVAPLMGLLGTVTGMIKTFQAITLYGAGD
                     PKLMAGGISQALVTTVLGLTVAIPTTLLHTIVSGRSKRVSQIIQEQGAGIIASHSEQE
                     TSQSM"
     gene            complement(6700..>7184)
                     /locus_tag="QGH35_01915"
     CDS             complement(6700..>7184)
                     /locus_tag="QGH35_01915"
                     /inference="COORDINATES: protein motif:HMM:NF023358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HJL81321.1"
                     /translation="KSIVMVSIINRQIVPVMTRMIESLEQFISLDVPFLKEERTNRVK
                     NLKEMMVRQDVTVAEKYRKVTEAYQIENDYGRTIETYKDTLEVDGAILELDFLRVGRI
                     SLMYQSVDGKVSGVWNQKKQSWEDADNSRNQIKMGLRIAKKMVPPDLVILPVDSPEAI
                     "
BASE COUNT         1983 a         1404 c         1200 g         2597 t
ORIGIN      
        1 cctacttcaa gtattgaaac tttgttcata attcaattcc atccttcttt tgagatttaa
       61 atatttgtga ctcagtgtat acaaatattg tgatattctc tatcttttac aaaacaaggt
      121 ttgtcatgaa tttaggaaga atctcaaccg ttacaattgg ttcgcatgat ctagattcaa
      181 tggttgaagc ctatagagat tatttaggct accgtcttac caagactgga aaaattacaa
      241 aactggaagc cacccattgg caagctaaaa acataactaa ttcagagtac gttattttgc
      301 agccagaaaa atcagatgat ttttcattta ggtttatcca gcaacccaat caacctgact
      361 atattccttt taaaagtgaa ggatggaatg cagctgagct aattgtccag aacgtagata
      421 aactttcaga agaattaata gattctccat ttgaaataat tgggcctcca gcagacctat
      481 cttttaataa gaacataaga gcaatgcaag taatcggccc ggcaaaagaa atactttatt
      541 tgaccgagtt taaaaagaaa attcccgaat tcgatagccc tgttcctagg tgttttgttg
      601 ataagacctt tatcgtgatt ctggcaggaa aatctctaga atccatgcag atttattttc
      661 atgatgattt tatgatagct aaagccccaa taatagaatc aaggattcgt tccatttcaa
      721 gagtatttga ccttccagaa gaaactcaat atccagcagc tgctttaact ttgaaagacc
      781 aatgcttcat tgaacttgac gaattaccta aagagggtat cagcagagtt tgtattgatg
      841 gttaccttcc ttcgggtatt gcaatggtga gtttcctttg ctacaaggag tcaaagtaca
      901 tcaataattt gtatccatca tctttacctg gttataaatc taatttttgt tcaatggagc
      961 aaggcagcgt cgaagaattg attgaactta tttattgttg agacaatatc tactttattc
     1021 aatgaattac tatgaaaatt ttattgttta gaaatatctt ttttaatgta gaatctttta
     1081 ggtcaattta atattttatt tcttaatatt agttgtggaa acaataataa ataacagtct
     1141 tataggtttg gttttattaa ccttagttgc tggcggtatt gcaggtatat tggctggatt
     1201 attgggagtc ggtggcggga tcgttattgt ccccctgctc tattatattc ttacattcct
     1261 aaactatgat caatccatca taatgcatgt tgctgtaggt acttctctgt ttgttattgt
     1321 tcccacgagt atccgttctg ccatgcaaca taagaaacac gatgcatttg atgcacgtat
     1381 ttttaagaaa tggtttgttc caatggttgc cggtactatc attggatcaa ttattgctaa
     1441 ctatgcttct ttcaaagctc taacgggttt gtttgcatgt attgcactta tcgtttctct
     1501 gcaaatgttt ttaaacagac aaaaaattaa tagaaatatt aagataccta aatttttgta
     1561 tcttattttt ccgtgtttca ttggcttaat atcggtcatg atgggcatcg gcggaggcac
     1621 tatgagtgtt cctgtgatga atcatttggg tgttgaaatg cgaaaagctg ttggaacctc
     1681 agcttctttt ggaactgtaa ttgcaatacc aggaacggta ggatttatag ttggtgggat
     1741 gggaaatcca ttattgcctc ccctttcatt tggctatata aatatcatgg cattctctct
     1801 tattgttcct gcgacattat tgacagttcc aattggtgtt aactatgctc accaaatctc
     1861 acagttaaaa ttaagaaagt tatttgcttc gtttttaggt ataaccgcta taaaaatgtt
     1921 gagcgatcta ttttagaaag taatagttga agattctttg cttttcaatt gttcttgtat
     1981 atttttgatt tgattttgta acaaagtgac tcgttctact tctttttcta ggaatttttg
     2041 ccaattggtt gctgatttgt aagatgaagg caatggacaa accagttttt tattttcaag
     2101 ctctttgcca attttctccc atcttgcttg tctatcaaag aggattaaaa aatcttcttg
     2161 ggtcatattt tctctaactg agcattcttc taatgcggct tctcttcgtg ctgtagcatc
     2221 ttcggttgca agattaaaag agttttttgc atcctcatac atggcttctt caaattgaca
     2281 catgccaaga gtaatatagg attcatcgtt cctctttaga cctcctttaa ggattccagt
     2341 ttttgctgca ttaacacaac ttttataatc tgctatgttt gccaatgatc gagctaaacg
     2401 cacgtctaat tctccttcat cggatagttt tgcggcttct cttaaagcaa taatagccat
     2461 ttggtcctct tgggctagaa accatgcctg tgataataat ctccagtttt tttcttccag
     2521 atcaacaatc ccttcatcta tgcctgtttg taaaataaca gcagctttat agggaacctc
     2581 ttttgagaga tagagttgtg taatttgaac atactcactg cttttatcta gaaacccctc
     2641 ttgataggcg gtggcataag ctgatatttg tttttcttct aattcgtctt gaccatatat
     2701 agctgacagt tgagtccaat actccttctt tgaccactct ataagcagac gttccaagac
     2761 ctctctaact ttaggaatat tctccagttc ataataggct gccctgagca gcaaccacca
     2821 attttcctta gtaggtccta ttgctaaatt gcttgctgat cttttagcac tatcaatagc
     2881 ctgttggatt tcttcttttt tttcagaact tctagtcttt aacaattttt gttttagttc
     2941 cgtctcaatt tgagttgcga atctaaaata atcaatttca tttttttctt cctcttctct
     3001 tctgaggtat ctaataccat tatcatcagc agccgagtta atattttttt gtagctgttc
     3061 tattctgaag gcatttcttg attctgctag gttgattgct gattgaatta ctgggatgga
     3121 ctctttatat ttttttagtt gatatagtgc aattcctttt aactcataca gatccgttct
     3181 tgggctttct tcggtagcaa gtaaagcatc taccgcactt atggcttttt cccactcttc
     3241 tagttgaaac cacaattggg caattgtaaa tctcgcttct gattgcaatc cttccggaac
     3301 agcctcttcg tttaaaattt tattataata gttaattgct tttttataat cctctctaag
     3361 gtattctaca tagccaaaaa aacgataaac atatgctctt tcataattgg atattctttt
     3421 ttgtaaaaga attccattta aaatttcagt gccttcttct atgcgttctg cttctattaa
     3481 ttcctgagcc tcaccaagtt ttttggcaac cttctctgac attgatccaa ctctctttgt
     3541 tcttctagct tgtttttctt cagcatcttg agcattgaca acagatataa cagagcaatt
     3601 actgatgtaa tcagtggata agaatataaa taaaaaggca aataggatta actttatttt
     3661 catgttattt ttctaattca aatgtaattt gtgtttgcac tcctgggaca tcaataggtg
     3721 tgccattgat aaccctaggt ttgtatttaa atttcaatgc ggccttgaga gatgcgcttt
     3781 gaaataaact actagtacat tcaactattt ttggatttct tgtagtgcca gtactggtaa
     3841 cgatgtattc gactatgcaa tacccctcta tgccccttga ttgagctctg ctggggtaaa
     3901 caggggctac tttaacaatc ggtaaatatt caccttctgc tgcattaaaa cctcctattt
     3961 cagcacctac cccgacattg gcattaatag ctccaacatt tagagtttcc gtattggtgt
     4021 cgatttcatc taactctgga ggagtatccg gtggtggctc ttctggttca gggggttttt
     4081 ttggtttgac tgtttttgtt tccaaagatt catctttttc gatgcgaaca aaatctacaa
     4141 aacgtccttc gtattttgaa gtaattgcac ctttaccggt agcgataaga atttgcatta
     4201 accacagtat tgcaaaagtt attatgagtg aaaataaaac tgagagagcc aacctttttc
     4261 ttggatcttc tatctgttct agaaacttgg aaatcgacct aaagcctgcc aatcttttat
     4321 ttctttgagc aatatcagcc attgatttta gtccggctca gctgctattg agacattaaa
     4381 tacacctgct tgacgtgctg aatccattac cagaacaaca gtttcatgga aggctttttt
     4441 atctgcttga ataactactg aaccttctgg attttctgca tgcagtcttt ctatatttgc
     4501 tctcactgct ctgggatcaa tacgtcttcg tgcaatccaa atctcattgt ttggaccgat
     4561 tgctaccaaa atgttggcat tctctttttt aactgccgtt ggggcatcag gacgatttac
     4621 atccaaaccg gattctttaa caaatgatgc agtgacaata aaaaatatta acatgatgaa
     4681 tacaacatca agcataggtg tcaaattaat ttcggtttct tcttcctgta ctgcctgaaa
     4741 taaatttttt ctcatttttt agacccctgt gattattcca ttattctgaa gttagattat
     4801 cttccaattc ttcaatttcc ttatttatct tttgattgag cacagtaatc aaaaagactc
     4861 ctgaaagggc tcccaccata cctgccattg taggaatggt cgcttttgaa acacccgaag
     4921 ccattgaacg tgcattacct gagccagaaa ttgccattac atcaaaaacc tcaatcatac
     4981 ccgttaccgt acccatcagt cccaataaag gacagactgc gacaagagtt tttatgattg
     5041 ccatgtattg attagcagac atattaaatt gagagattag tttttctcta atagcatgag
     5101 actcccatgt tttcttttgt gctctagcat tccaggtgga ttgtatatct gatttttgta
     5161 tctttaatcc cgattgaaag aaagctagtc tttccagtat taatatccac ataaagaaaa
     5221 ttacgaaagc tatcatgctc aacacatcac ctcccatctc taggaaatca cggacagaga
     5281 ttataaggtc ttgaatcatc tcaactaata ctcattatta accagtctct gcttacattg
     5341 attggcttgt ctcttgttct gaatgagaag caataattcc ggcaccttgt tcttggatga
     5401 tttgtgaaac tcgtttgctt ctgccactga caatggtatg caagagcgtt gttggaattg
     5461 caaccgttag tccaagtaca gttgtaacca gtgcttgtga aataccacct gccattaatt
     5521 taggatcacc tgctccatat agtgtgattg cttggaatgt ttttatcata cctgtaacag
     5581 ttccaagtag acccataaga ggtgcaacga ctgagatcac ttttattaat ataagtcctt
     5641 tattaagttc aggagtttct ttgaatacag cctctgccat ttttaactct aatgtttctg
     5701 tgtcagtgtt tttattctca tcgtaaacac taagcactcg tcctaaagca ttatctttac
     5761 taacgacatc actttttagt tgtgcggtta cttttctatc agcgtcaacc aaacgaataa
     5821 gcctttcgat tgctatacca accccaaaaa gacccagtaa aataatcaga taaccgacta
     5881 taccgccttg ttgtatgcgc tctatcaaat ttggtcgtgc cacaagcgac attagaactc
     5941 cacctaaagt aggatcaagt ccaaaggcaa ctttttcacc tgaagcgtta aatagatcag
     6001 cggtactacc ggtaaatctt cttccttctg gttgccttgg tatttctata acattacctg
     6061 tttcaggttc atagttaaga tattttccat ttgcaacgat attgaaagaa cccactctaa
     6121 tgacttcagt tttgtttttg cctccttcta catcgatgac atcagttgaa aatttgatca
     6181 cttttcctga ttctgtcatc tctctctgaa gctcgtacca caacctttca atttctggaa
     6241 tacttggtaa tttggagttg cttcctattt tctttgctaa gtcttctaaa aattggccac
     6301 gatctggata ttgaatattt gttaaagagt tttcaaatcg acttcttgca tctcctgaaa
     6361 cctgttgcat aacgccaaat agttctttta gtgcccctaa tcgttcattg agggtattct
     6421 ggagttcgtt cagctctttg tcattatctt caaagattac ctccagtgtc gcacttttta
     6481 cttcttccgc ttccttttgt ttgacggaat catcgagtaa ctgttcctgt tgtgatcttt
     6541 tttgtcgaaa ctcgttttcc ctttttctat tgtctgcact atctagaatt tttccttgtt
     6601 caacattgtc tagcaactga tctatattcg tcgcttgttg agcgtgtatt gaaaaagaaa
     6661 atgctaaaaa taggcttgta attatcaata agtttttcat tatattgcct ccggagagtc
     6721 aacaggtaga atcactaaat caggaggaac cattttttta gctattctta agcccatttt
     6781 tatttggttg cgggagttgt ctgcatcttc ccaggactgt ttcttttgat tccaaacacc
     6841 tgaaactttt ccatcaacag attgatacat aagagaaatt cttcctaccc ttaagaaatc
     6901 gagttctaga attgcgccat caacttcaag ggtgtcttta tacgtttcaa tggttcgtcc
     6961 ataatcattt tcaatttgat aggcttccgt cactttccta tacttttcag ctacagttac
     7021 atcttgtctg accatcattt ctttaaggtt ttttacacga tttgttctct cttcttttaa
     7081 aaaaggaaca tcaagtgata tgaattgctc aagtgattca atcattctgg tcatcacagg
     7141 aacaatttgt ctgttgatta ttgaaaccat gacaatagat tttt
//
LOCUS       DASCTH010000088         2773 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000126368, whole genome shotgun sequence.
ACCESSION   DASCTH010000088 DASCTH010000000
VERSION     DASCTH010000088.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000126368"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            54..1088
                     /locus_tag="QGH35_01920"
     CDS             54..1088
                     /locus_tag="QGH35_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007625395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HJL81322.1"
                     /translation="MKSKQKNYLFYIEHDYSYEILRPLQKEIQKQGDRVCWFLVGKQI
                     NNHLSEAERELKTVEEVIEYNPQACFVPGNTIPNFIPGLKVQVFHSLEWKKKTTFRIR
                     NCFDLYCTHGPSTTNTFNVLKQKHQHFDVIETGWPKTDALFEAIPLNFPEARGPIILY
                     APTFSPKLTSAAELHDSINKLSKQQSWFWIIKFHPKMDPKLVDQYREIEGKNLKIFET
                     DSIAPLLKSAELMVSDTSSVIGEFALLQKPTITLNNAMPDDYIINISNHTELESAINT
                     VLTGKTAIYEEVAAYAKQLHPYYDGQSSRRVLQAVTNISEKNLNYLKPKPRNYFRHIK
                     MRMKLNYWKG"
     gene            complement(1085..1618)
                     /locus_tag="QGH35_01925"
     CDS             complement(1085..1618)
                     /locus_tag="QGH35_01925"
                     /inference="COORDINATES: protein motif:HMM:NF024698.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJL81323.1"
                     /translation="MAVIKTERLTIKSPELDDVGAMVALINNWEITKWLSNVPYPYLQ
                     SDGIGFVKRSKQKHEIGSNFNYLVFLQDTLVGGVGLSLQENGIYNLGYWVGKQYWGQG
                     IATEASYALLAFGFDNLRQTKIQAGYFDGNDASAHVLKKLGFVSLGRTKLFSKSQNQV
                     LCDHMMYLEKCGFENLD"
     gene            complement(1605..2696)
                     /locus_tag="QGH35_01930"
     CDS             complement(1605..2696)
                     /locus_tag="QGH35_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HJL81324.1"
                     /translation="MESNKNSLTPDSICVIRLSALGDCCHALPAVQTLRDNLPNCRLT
                     WIIGKTEYQLMDGLEDIEFIVIDKSNLIRSLIKLNSKLKQRKFDVLLHMHASMRANIV
                     SYFVRANRKIGFDKNRARDLQQLFCNESIAPKKNQHVTDGFFEFLQHIGITQQTPRWN
                     LPIPNSAIEHANRQLDNTKITCVISPCSSQRYGDANRNWSLENYITIINHLIQKYDIQ
                     VILTGGQTIIESSYGEQLTEYFQNRIVNLIGKTSVKQLVGIINEADFVLCQDSAPAHI
                     STATNTPVIGLHAFTNPLRSGPYFCQEWVVNAYPEAVKKFLHKDVSEVRWSQKIKHPD
                     AMNLITTKVVIEKIDQMLLMEIEKNNGCY"
BASE COUNT          873 a          529 c          487 g          884 t
ORIGIN      
        1 tgtgtaatcg taacaattgc tatttgtgat ccagttctga ggaaatcaat cttttgaaga
       61 gcaagcaaaa aaactatttg ttttatatag aacatgatta ttcttatgag atcttaagac
      121 cgcttcaaaa ggagattcaa aaacaaggag accgtgtttg ttggtttttg gtaggtaaac
      181 aaatcaacaa tcatctttct gaggccgagc gtgagttgaa aacagttgag gaggttattg
      241 agtataatcc tcaagcttgc tttgtccctg gcaatacaat ccctaatttt attcctggtt
      301 taaaagtgca agtattccac agcctagaat ggaagaaaaa aacgacgttt agaatcagga
      361 attgttttga cctctattgc acacatggtc ctagcaccac taatacattc aatgttctaa
      421 agcaaaagca ccagcacttt gatgtcatag aaacaggatg gccaaaaaca gatgctttat
      481 ttgaagccat tcctctcaat tttcccgaag caaggggacc aataatattg tatgcaccta
      541 cattctcacc aaaattgact tcagctgcag aacttcatga ttcaataaat aaactctcta
      601 aacaacaatc gtggttctgg atcattaaat tccatccaaa aatggacccc aaattggtag
      661 atcaataccg agaaattgaa ggcaagaatt taaaaatttt cgaaacagat tcaattgccc
      721 cattgttaaa atcagcagag ttgatggttt cagatacttc ttcagtcatc ggtgagtttg
      781 ccttgctaca aaaacctacc ataactttaa ataatgcaat gccagatgac tacatcataa
      841 atatttctaa tcacacagaa ttggaatcgg ccattaatac cgttctcact ggtaaaacag
      901 caatctatga agaggttgca gcgtatgcta agcaattaca tccttattat gatgggcaat
      961 catcaagaag agttttgcaa gcagtgacca atatcagtga aaagaatttg aactatctta
     1021 aaccaaaacc taggaattat tttagacaca ttaagatgag aatgaaatta aactactgga
     1081 agggctagtc aagattttca aaaccacact tttctagata catcatatga tcacacaaga
     1141 cttgattctg tgatttagaa aaaagcttcg ttctgccaag gctaacaaac cctaattttt
     1201 ttaaaacatg agctgaggca tcgttgccat cgaaataacc ggcttggatt tttgtttgtc
     1261 ttaagttgtc aaaaccaaat gcaagcagtg cataacttgc ttccgttgca atgccttgac
     1321 cccagtactg cttacccacc cagtaaccca aattataaat tccattttcc tgaagagaaa
     1381 gaccaacccc acctacgaga gtatcttgaa gaaaaaccaa ataattaaaa tttgatccta
     1441 tttcatgctt ttgctttgat ctctttacaa aaccaatgcc atcgctctgg aggtaggggt
     1501 atggaacgtt tgagagccat tttgtgattt cccaattatt aatcaaggca accattgcac
     1561 ccacatcatc gagttctgga gattttattg ttagcctctc tgttttaata acagccatta
     1621 tttttttcaa tttccatcaa tagcatttga tcaatctttt ctatgactac cttggttgtg
     1681 attagattca ttgcatctgg atgtttgatt ttctgactcc acctgacttc actgacatct
     1741 ttatgaagaa attttttaac tgcctcggga taagcattga cgacccactc ttgacaaaag
     1801 tatggaccgc ttctcaaagg attggtaaat gcgtgcagcc caataacagg agtatttgtc
     1861 gctgttgaaa tatgtgcagg tgctgaatct tggcaaagga caaaatctgc ttcattgata
     1921 attcctacta attgtttaac cgaagttttt ccaattaggt ttacaattct attttgaaag
     1981 tattctgtta gttgttcacc ataacttgat tcaattattg tttgcccacc tgtaagaatg
     2041 acctgaatgt cgtatttttg aatcagatga ttaatgattg ttatgtaatt ttccaaagac
     2101 cagtttctgt tggcatcacc ataccgttga ctgctgcaag gactgatgac gcaagttatt
     2161 tttgtgttat ctagttgtcg attggcatgt tcaatagccg agtttggaat gggtagattc
     2221 caacggggcg tttgttgggt aataccaatg tgttgcagaa actcaaaaaa cccatccgtg
     2281 acgtgctgat ttttctttgg tgcaattgat tcgttgcaga ataactgttg taaatctcta
     2341 gcacgatttt tgtcaaaacc aatctttcta tttgctctta cgaaatagct aactatattt
     2401 gctctcatag aagcatgcat atgcaataag acgtcaaatt ttctttgttt taatttagaa
     2461 ttgagtttta taagagatct aataaggttt gatttatcaa taacaataaa ctctatgtcc
     2521 tcaagcccat ccatcagttg gtattctgtt ttgccgatga tccaagtcaa tctacagttt
     2581 ggaaggttat cacgcagtgt ctgcaccgcc ggtaatgcat ggcagcaatc gccgagagct
     2641 gataatctga ttacacaaat actatctgga gttaaactgt tcttattgga ttccatttta
     2701 tggctaagca acttataaaa atcaatcaat ctattatcgt atatgatgat cgtttaatca
     2761 gccaaccgga tga
//
LOCUS       DASCTH010000089         2081 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000126843, whole genome shotgun sequence.
ACCESSION   DASCTH010000089 DASCTH010000000
VERSION     DASCTH010000089.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000126843"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="QGH35_01935"
     CDS             complement(<1..1109)
                     /locus_tag="QGH35_01935"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HJL81325.1"
                     /translation="MYSYNEFDKNFVRQRVNEFKGQVQRRIDGVLTEDEFLPLRLQNG
                     LYMQIHSYMLRVAIPYGTLNSKQMRQLALIAEKYDKGYGHFTTRQNIQYNWIKLSDVA
                     DVLSDLAEVDMHAIQTSGNCIRNVTSDHFAGAAVDEIEDPRPVAELLRQWSTGHPEYA
                     FLPRKFKIAVTGSSHDRVAIAFHDIGLCTVRNDDGEIGYKIYIGGGLGRMPRIGRVLS
                     EFIPKIQLLNYIDSIIHIYNRFGRRDNKHRARIKILVDELGLNEARRQIYEYYNSIKN
                     ESRTPFKDIIKFIMDGFSPPDFSTAKEHNLEKDFLNSLSLKNWAETNLTPHRDSNHVI
                     VSVSLKKPGATPGDATAKQMRVLADLAEQYAYDELR"
     gene            complement(1119..1409)
                     /locus_tag="QGH35_01940"
     CDS             complement(1119..1409)
                     /locus_tag="QGH35_01940"
                     /inference="COORDINATES: protein motif:HMM:NF022464.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HJL81326.1"
                     /translation="MRKILTANYLLSGKVIYLTSEKKWIEYLENAAVFDNQEDADNAL
                     TFADTQINKLQSAYLIDVELTSNGKPKPISNQELIRATGPTNYFHGKQIEKN"
     gene            complement(1411..>2081)
                     /locus_tag="QGH35_01945"
     CDS             complement(1411..>2081)
                     /locus_tag="QGH35_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005983493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HJL81327.1"
                     /translation="SGWSQNDINILLISKAKTGQKVVRLKSGDAGVFSRLDEEITALD
                     QAGLPFEVIPGITTAAAAAADMGVSLTRRGRNKQVRLLTGHDIDGFSDHDWKSLALDG
                     AVAAIYMGVKTISHLQNKLLRHGALSDTPITVAQNVGRKNAIWVASSISTLVNDCRHH
                     KITGPAILMLGLHPHIQKTSLISKKLQNSKDVFLYKDLSINEVLPQLVIKNKKSATTD
                     KKFL"
BASE COUNT          615 a          411 c          358 g          697 t
ORIGIN      
        1 cgcagctcat cataagcata ttgttcagct aaatctgcca aaactctcat ttgtttagct
       61 gttgcatcac caggggtagc tcctggtttt ttcaaggaaa cagacactat tacatgattt
      121 gaatcgcgat gaggcgtaag atttgtttcc gcccaattct tcagtgataa agagtttaaa
      181 aaatccttct ccaaattatg ttcttttgca gtagaaaaat caggtggaga aaacccatcc
      241 atgataaatt taataatatc tttgaaaggc gttcgagatt cgtttttgat ggaattataa
      301 tactcgtaaa tctgacgtct tgcttcatta agaccaagtt catcaaccag aatttttatt
      361 cgagctctgt gtttattgtc acgtcgtcca aaacgattat agatatgtat aatggaatct
      421 atatagttca ataattgtat ttttggtatg aattccgata acacacgacc aatacgcggc
      481 attctaccta atccacctcc aatataaatc ttgtatccta tttctccatc atcatttctc
      541 acagtacaaa ggccaatatc atgaaatgca atggcgactc tatcgtgtga tgagccagtg
      601 acagcaattt taaacttacg tggtagaaat gcatactccg ggtgtccagt tgaccattga
      661 cgtagcaatt cggctacagg tctcggatcc tcaatttcgt ctacggctgc acctgcaaaa
      721 tgatctgatg ttacattgcg aatacaatta ccagatgtct gaatagcatg catatcaact
      781 tcagctaaat cagacaacac atcagctaca tcggacagtt ttatccaatt atattgaata
      841 ttttggcgag ttgtaaaatg accatatcct ttgtcgtatt tctcggcgat taatgctagt
      901 tgacgcattt gtttactgtt tagagtgcca tatggaattg caacccttaa catatagcta
      961 tggatttgca tatatagacc attttgaaga cgaagtggta agaattcatc ttctgtcaaa
     1021 acaccgtcaa ttctcctttg tacctggcct ttaaattcat tcacacgctg gcgcacaaaa
     1081 tttttatcaa actcgttata tgagtacatt ttattatgtc agttcttttc tatttgcttg
     1141 ccatgaaagt agtttgtggg accagttgca cggatcaatt cttgattgga tatgggtttt
     1201 ggtttaccgt ttgatgttag ctccacatca attaaatacg cactttgtag tttgtttatt
     1261 tgagtatctg caaatgttag agcattatca gcatcttctt gattgtcaaa tacagcggca
     1321 ttttctaaat attcaatcca ttttttttcc gaagtcaaat agatcacttt tccactaagt
     1381 aaatagtttg cggttaaaat ttttctcatt ctacaaaaac ttcttatctg ttgttgcaga
     1441 ttttttattt ttaattacca actgtggtaa aacttcatta atagacaagt ctttgtacaa
     1501 aaaaacatct ttagaatttt gtaatttctt agagataaga gatgtctttt gaatatgtgg
     1561 gtgtaatcct aacatcaata ttgctggacc tgtaattttg tgatgcctac aatcattcac
     1621 aagtgttgat atgctagaag ccacccaaat agcatttttt cttcctacat tctgagcaac
     1681 tgtaatcggc gtgtcgctta atgcaccatg tctgagcaac ttgttttgta gatgggaaat
     1741 tgttttcacg cccatataaa tggccgccac agcaccatcc aaagcaagag acttccaatc
     1801 atggtctgaa aatccatcaa tatcatgacc agttaataaa cgtacctgtt tattgcgtcc
     1861 acgacgcgtt aatgagacgc ccatatcagc agctgctgct gctgcggttg taatacctgg
     1921 tatgacttca aagggtaaac cagcttgatc aagggcagta atttcttcat caagacggct
     1981 aaaaactcca gcatcaccag atttcagacg aacaaccttt tgtccagttt tagctttgga
     2041 tatcagaaga atatttatat cattttgaga ccaaccagat c
//
LOCUS       DASCTH010000090         4325 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000127680, whole genome shotgun sequence.
ACCESSION   DASCTH010000090 DASCTH010000000
VERSION     DASCTH010000090.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000127680"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="QGH35_01950"
     CDS             complement(<1..1316)
                     /locus_tag="QGH35_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013122.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HJL81328.1"
                     /translation="MNNQWYQEPKDMLTERGLAGLDLETTGINRNIEYERPTSAAILE
                     STDNVENPDIVIDNKCRLPYHVLPDAGALRITNINPMELMNEELSLHQLIGKIVDYFL
                     SKPNKILATYNGATYDLIILRHSFFSSLYNPYILSSAFEDRIHIDLYKIAQSIYCFAP
                     KSIAFFKDATGKVVLKQEMLAKENGIDPGNSHAALDDVKALLKLANLFEQQAPEIFFS
                     AIASGNKSRAVKLMTEQLYFTYGEVKYKERLAVKRTPTFICEDPTYSNRMIFFDLCFN
                     PDDYLYMTAEEISTLINKKNSPLFSIKSNSSPVILHPSISDKNNLNEEKATHRASIVQ
                     ASNGFKQNVYLACDIISKKRTPWTMLSFPESQIYAQFIDKSDRLLCDAFLKNININDR
                     LEIVNQLNDDRLIDFAKRILAFEHQDCGPKLIMEYQEFEAMRLLNEG"
     gene            1444..1578
                     /locus_tag="QGH35_01955"
     CDS             1444..1578
                     /locus_tag="QGH35_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81329.1"
                     /translation="MMSIISCGQQGDLYLPEDPSKEQTQSTVLKSPSLIPAEQIIINE
                     "
     gene            1571..4258
                     /gene="polA"
                     /locus_tag="QGH35_01960"
     CDS             1571..4258
                     /gene="polA"
                     /locus_tag="QGH35_01960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015005011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HJL81330.1"
                     /translation="MNDIKSLLLVDGSSYLYRAFHALPGLTSPTGQPTGAIYGVLTML
                     QKLIKAEKPDLVGIIFDAPGKTFRHDLFPDYKANREKTPEDLVVQIEPLLTAIQNLGL
                     PLIRIQGVEADDVIGTLARQAENNNIQTLIATGDKDFAQLVSERIKLIDTMKNITTDQ
                     SGVLDKFGLRPEQIIDYLALAGDPSDNIPGIPKVGPKTAAKWLQQYTDLNGVIKHAEE
                     IVGKVGEALRTSIPDLALYQKLATIDCNVELEHTIHDLVIGRAQEEALFEQLNTLGLH
                     RLIKQFMISKPETKTQKINNYHGILTKKEFNTLLSLLDKSDIAAIDTETTSLNYLQAE
                     IVGISFAITAKEAYYIPLLHNYDEAPTQLDRAYVLSKLKPWLENSNYKKIGHNLKYDS
                     HIFANHGITLNGMAFDTMLESYVLNSTATRHNLNAVAKRYLNQDTISYEEVAGKGAKQ
                     ISFNKVSLEDAIPYAAEDADVCFRLHHALYPKLQNIDALIELYSNIESPLVAILQQVE
                     RNGVLIDKLKLEKQSDQFAAKLNELEEQAYHLVGSEFNLSSPKQLQEILFDQMGLPVL
                     KKTPKGQPSTAEGVLQELAHDFPIAQTILEHRTIAKLKTTYTDKLPLLINKETQRVHT
                     SYHQAVTSTGRLSSSDPNLQNIPIRSAAGRQIRQAFIAPKGWKILAADYSQIELRIMA
                     HISKDRGLLDAFEKELDIHQATAAEIFDTDLEVITKEQRRSAKAINFGLMYGMSAFGL
                     SRQLSISRQDAEQYIELYFARYPRVKDYMESAVRQARESGFVETVFGRRLYLPDIDAK
                     HYQRRQYAERSAINAPMQGTAADIIKKAMIELQDQLEDSSKEAKMIMQVHDELVLEVQ
                     DNAVEAISSLVSQVMSQAADLEVALKVDVGVGNTWDEAH"
BASE COUNT         1379 a          828 c          844 g         1274 t
ORIGIN      
        1 ccctcattta gcagtctcat agcttcaaat tcttgatact ccattattaa tttaggacca
       61 caatcctggt gctcaaaggc cagaatcctt ttagcaaaat caatcagccg atcatcgttt
      121 aactgattga caatttccaa gcgatcattg atattgatgt ttttaagaaa agcgtcacac
      181 aataatcggt cagacttatc aataaactgt gcatagattt gggattctgg aaaactaagc
      241 atagtccaag gggtcctttt tttactgata atgtcacacg ctaaataaac attttgtttg
      301 aatccattgc ttgcttgtac tatagaagct ctatgtgtag ccttttcttc attcaggtta
      361 ttcttatcac tgatgcttgg atgcaatatc actggactgc tgtttgactt gatggaaaaa
      421 agaggtgaat tttttttatt gatcaaagta gaaatttctt ctgctgtcat atataggtaa
      481 tcatcagggt taaagcaaag atcaaaaaaa atcatccgat tggaataagt gggatcttca
      541 caaataaatg ttggcgttcg tttaactgcc aaacgttctt tatacttcac ctcaccataa
      601 gtgaaataca attgttcagt catcaatttt actgctctgg atttgttacc tgaagcaatt
      661 gcactaaaaa aaatctctgg cgcctgttgc tcgaataaat tggctagttt tagcaaagct
      721 ttgacatcgt ccaatgctgc atgggaatta cctggatcaa tgccgttttc ttttgctaac
      781 atttcttgtt tgagcactac tttgccagtt gcgtctttga aaaaagcaat gcttttaggt
      841 gcaaagcaat aaatagactg tgctatttta tatagatcaa tgtgaatgcg atcttcgaac
      901 gcgctggata atatatacgg gttatacagg gatgaaaaaa aagaatgcct aagaataatt
      961 aggtcataag tagcaccgtt gtaggtggcc aatattttat ttggtttgct gagaaaatag
     1021 tcgacaattt ttccaatcag ttgatgcaat gataattctt cattcatgag ctccattgga
     1081 ttgatattgg tgattctgag agcaccggca tctggtaaaa cgtgatacgg caatcggcat
     1141 ttgttgtcta tgactatatc tggattttcg acattatctg tggactctaa aatagcagca
     1201 gaagttggtc gttcgtattc gatattacga ttgatccctg tagtctctaa atccagacca
     1261 gccaagcctc tctcagtcag catgtcttta ggttcttggt accattgatt gttcatatct
     1321 tattaaccaa ttatttttat gagtccagag atgataaaat aaacactcag agctattcaa
     1381 aagttattac cagatatgtt atcaaaaaat ttttttataa aaggaggctt ttctctgatt
     1441 ctaatgatgt ctatcatatc ttgtggccaa caaggcgacc tatacctgcc tgaagatcct
     1501 tcaaaagaac aaacccaatc aactgtttta aaatcaccct cgttgattcc agctgaacaa
     1561 atcattatta atgaatgaca taaagtcact attgttagta gatggttctt cttacctata
     1621 ccgtgctttt cacgctttgc ccggactcac atcgcctaca ggtcagccaa caggtgcgat
     1681 atatggtgtc ttaaccatgt tgcaaaaatt gataaaggct gagaagccag atttagtggg
     1741 tattatattt gatgcaccag ggaaaacttt tagacacgac ttgtttccag attacaaagc
     1801 caatcgtgag aaaacccctg aggatcttgt tgttcaaatt gaaccacttc taacagctat
     1861 ccaaaatctt ggtctgccgt taattcgtat acagggcgtt gaagcagatg atgttattgg
     1921 aacattggct agacaggcag aaaataacaa tattcaaaca ttaattgcaa caggcgataa
     1981 agattttgca cagttggttt cagaaaggat caagttgatt gataccatga aaaatatcac
     2041 cacagaccag tcaggtgtac tggataaatt tggcttgcgg cctgagcaaa taattgatta
     2101 cctcgccctt gctggggatc cttcagataa tattcctggt attcccaaag tgggtccaaa
     2161 aacagctgct aaatggttgc aacagtatac agacctaaat ggggtcatca agcacgctga
     2221 agagattgtc ggaaaggtgg gtgaagcgct tagaacttca atacccgatt tggcgttgta
     2281 tcaaaaactc gccaccattg attgcaatgt tgagcttgag cacactatcc atgatttggt
     2341 cattggtcgt gctcaagaag aagcgttatt tgagcaatta aacactcttg gtctccaccg
     2401 cttaataaaa caatttatga tttcaaagcc agaaacaaag acgcagaaga tcaataatta
     2461 tcatggcatc ttaaccaaaa aagaatttaa tacattattg agtttattgg acaagtccga
     2521 tattgctgct attgatactg aaacaacaag cctaaattac ttgcaggctg aaattgttgg
     2581 tatctctttt gcaataactg ccaaggaggc ttattacatt cctttattac acaactatga
     2641 tgaggcacct actcaattgg acagagctta tgtgctgagc aaattaaaac cttggttaga
     2701 aaattccaat tacaaaaaaa ttggccataa tcttaaatac gacagtcata tttttgcaaa
     2761 ccatggaatt acattaaatg gaatggcttt cgataccatg ttggaatcct atgtgctcaa
     2821 tagcacagca accagacaca acttaaatgc agtagcaaaa cgctatttaa atcaagacac
     2881 aatcagttat gaagaagtcg ctggaaaggg ggcaaagcaa attagtttta ataaggttag
     2941 ccttgaagat gcaatacctt atgcagcaga agatgccgat gtgtgttttc gcttacatca
     3001 tgcgctgtac ccaaagctgc aaaatataga tgcattaata gagctttata gcaatattga
     3061 atcccctttg gttgcaatac tgcagcaggt ggaacgcaat ggcgtcttga tcgataaact
     3121 aaaactagaa aaacaaagcg atcaatttgc agccaagctc aatgagctcg aagaacaagc
     3181 ctatcatttg gttggatctg agtttaatct aagctcaccg aagcaactac aggagatttt
     3241 atttgatcaa atgggtcttc cggtattaaa aaaaacacca aaaggtcagc catcaaccgc
     3301 agaaggtgtg ttacaggagt tagcacatga ttttcccatc gcgcaaacta ttctagaaca
     3361 ccgaaccatt gcaaagttaa aaaccaccta taccgataag ttaccgcttc tcatcaacaa
     3421 agaaacacag agggtccaca cctcttatca tcaagcggtt acttcaacag gtagactgtc
     3481 ttcttcggat ccaaatttac aaaatatacc cattagatcg gcagcaggaa ggcaaattag
     3541 acaagcattt attgctccca agggctggaa aatattggca gcagattatt cccaaatcga
     3601 acttagaatt atggcccata tttctaaaga tcgggggtta ttggatgctt tcgagaaaga
     3661 actagatatc catcaggcaa ctgctgctga aatttttgat actgacctag aagtcattac
     3721 aaaagaacaa agacgatctg cgaaagccat caattttgga ttgatgtatg gaatgtcagc
     3781 attcggactt tctcgtcagt tgagcatctc acgccaggat gctgaacaat acattgaatt
     3841 gtattttgct cgatacccaa gagtaaaaga ctatatggag agtgcggtcc gccaggcaag
     3901 ggaatctggt tttgttgaaa ctgtttttgg cagaaggctt tatttaccgg atattgatgc
     3961 aaaacactac caacgcagac aatatgctga acggagcgca attaacgcac ctatgcaggg
     4021 aacggcagcg gatattataa aaaaagcaat gattgagctt caagatcaat tagaagattc
     4081 ttccaaagaa gccaaaatga ttatgcaagt ccatgatgag ctggtgctag aagttcaaga
     4141 caatgccgtt gaggcgatca gctctctcgt ttcacaagta atgtcgcagg ctgctgattt
     4201 agaagtggct ttaaaggtag atgttggtgt tggcaacacc tgggacgaag cgcattaggg
     4261 agcctcattt ttttaacatt tcaatcaacc tattttgtgc gatttctaac cccattttct
     4321 tggtt
//
LOCUS       DASCTH010000091         4868 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000129205, whole genome shotgun sequence.
ACCESSION   DASCTH010000091 DASCTH010000000
VERSION     DASCTH010000091.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000129205"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="QGH35_01965"
     CDS             complement(<1..902)
                     /locus_tag="QGH35_01965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HJL81331.1"
                     /translation="MIPTDTLSKYLRRSIPILFLFIWNVHAVVFVPKAPTIPVKSYIL
                     MEANTGKIIAKYEENKKIQPASITKLMTAYSVFEALENEQISLNDLVRISQRASSIGG
                     STMFLDPSMKVSVEELLQGMIIISGNDASIALAEHTGGSEDAFVEFMNVYAESLGLKN
                     TQYKNSTGLDDEGHYSSAIDIALLANEIMKKYPQYFQWYSQPSFTFDKAKDPLTNEPI
                     IQYNRNKLLQRNSSVDGMKTGYTSSAGYCLVATANKHNMRLIAVVTGANSDVERTDSA
                     AALLNYGFRFFETTTIVDSKTSYA"
     gene            complement(994..1815)
                     /locus_tag="QGH35_01970"
     CDS             complement(994..1815)
                     /locus_tag="QGH35_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HJL81332.1"
                     /translation="MNSRSEKISKYTLPIIFIVLTSNCSYVPNVVKQQTKSSQISVLQ
                     NDCTIEREKGYVYKVRGKKYKVLRSARGYKKRGVASWYGPNFDGKSTACGETYDMLEY
                     TAAHKTLPLPSYLRVRNLKNGASVIVKVNDRGPFKDNRIIDLSYAAAKGIGMIDDGTA
                     FVELRSITRQEAARSMYYENKSSIAKEKIAAITQLSNSNIYLQIGAFKTKSNARKLKK
                     QIEEKDIDSVYMERVWTKIGILYRVRIGPIKGLNRYDEIVKSLISLSLDITLVTN"
     gene            complement(1812..2804)
                     /gene="mltB"
                     /locus_tag="QGH35_01975"
     CDS             complement(1812..2804)
                     /gene="mltB"
                     /locus_tag="QGH35_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HJL81333.1"
                     /translation="MNYKKTIINILLLSMIQLIICPSVLQAKESNEAIKLELIKELVT
                     DHGFDEQYITAVFEKATYLPKIINLISKPAEKTKTWNEYRRIFVTPARIAAGVIFAKQ
                     HEQLLLKAAQETGVPVDIILGILGVETYFGRIQGNFKVIDSLYTLVTGYPPRSAFFKE
                     QLINLFYLAREQGIAIEEIKGSYAGAMGASQFIPSSYRAYAVDGDNDSMIDLFENWSD
                     IIMSIANYLQQNGWRRNENIISQTSLDDEQLKTFASKTLKPQYTIETLNDNGITFESN
                     LNEDSPAQVILLEGDSEKNYVGFHNFYVITTYNRNVMYALSVIEVGRSIQSKIQ"
     gene            complement(2801..3919)
                     /gene="rodA"
                     /locus_tag="QGH35_01980"
     CDS             complement(2801..3919)
                     /gene="rodA"
                     /locus_tag="QGH35_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HJL81334.1"
                     /translation="MVVENNISWNHPTEGILYKFHIDPSLLLAVMLIFIISLIAIYSA
                     SNSNMDVLINQSIRIIIAIISMVTIAQFSPLTYERIGFWLYLLCVALLVMVLLFGETI
                     NGATRWLNIGINFQPSELMKIAMPLMIAGYIARDKIPPSNQSIIFSLLIVLIPTELIL
                     LEPDLGTAVLIAFSGLVIIFLSGIRKRYLAIAMAAFFASLPLIWSSMHPFQKNRVLTF
                     LNPENDPLGAGYHIIQSKIAIGSGGIFGKGWLSGSQGQLDFLPERTTDFIFSVIAEEF
                     GFLGIIPLLSIYLFIIGRGIIIAINSQNLFSRLLASSISLTFFVYVFVNIAMTTGILP
                     IVGIPLPLISAGGTSMVTIMLGLGMLMSIQTHRRLVEK"
     gene            complement(3919..>4868)
                     /locus_tag="QGH35_01985"
     CDS             complement(3919..>4868)
                     /locus_tag="QGH35_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HJL81335.1"
                     /translation="GISSSEYRSLQTNKNKPLFNRAIAGQYPPGSTIKPIIALAGLQQ
                     GFIDPTKLEPCDGAFYLPNYSRPFNDWREHGPVNVKEAIQASCDVFFYKTAINIGITS
                     TSNFLKKFNLGSLTGIDISYEKSGVVPNPEWKKTIKNQNWYPGDTVNNGIGQGDMLAT
                     PLQLAIATTIIANKGKAYQPHLVKGIKNTQTKTIEPVPLSVLTDITDINNDHWNIIHQ
                     GMSAVINQRGGTAFGIFENNYPLAGKTGSSQLYSIEKTKGKKIPDNLKDHGLFIGFSP
                     LDNPKIALAIIVENGVSGRTAAAPIAKEILDVFLDREKP"
BASE COUNT         1449 a          896 c          835 g         1688 t
ORIGIN      
        1 tcggcatacg atgtttttga atcaactatg gttgttgttt caaagaatct aaagccatag
       61 tttaataaag cagctgctga atctgttcgt tcaacatctg aatttgcacc ggtcaccaca
      121 gcaatgagtc gcatgttatg tttattagcg gttgcaacta gacaataacc tgctgaggat
      181 gtataccctg ttttcattcc atccaccgaa ctatttcttt gcagtagctt gtttcgattg
      241 tactgaatga ttggttcatt cgttaatggg tcctttgctt tatcgaatgt aaaagatggt
      301 tgagaatacc attgaaaata ttgaggatac tttttcatga tctcattggc taaaagagca
      361 atatcaatgg cagaggaata gtgtccttca tcatcaagtc ctgttgaatt tttatactga
      421 gtatttttta gaccaagact ttcagcataa acattcatga actcaacaaa tgcatcttca
      481 gatcctccag tatgttctgc aagggctatg ctggcatcat taccagaaat aataatcatg
      541 ccttgtagta actcctcaac gctgactttc attgagggat ctaaaaacat agtagaacca
      601 ccaattgaag aggctctttg actaatacga actagatcat tgagactaat ttgttcattc
      661 tctagagctt caaaaacgga ataagccgtc attaatttag tgatactcgc tggttgaatt
      721 ttcttatttt cttcgtactt tgcaatgatt tttcctgtgt ttgcttccat taagatatag
      781 cttttcacag ggattgtagg agctttgggc acaaacacca cggcatgaac attccaaatg
      841 aataaaaata agattggaat agatcttctc agatattttg aaagtgtatc tgtgggtatc
      901 atctggttaa gaatagtttt tatcaagttc aatattcaat gttgcaaacc atatcataaa
      961 cgaaaaaata tgagtattga gctttatcat taattaattt gtaaccaagg taatatccag
     1021 tgacagtgat attagtgact ttactatttc atcgtagcgg tttaaacctt ttataggtcc
     1081 tattcggacg cgatacaata ttccaatttt agtccatact ctttccatat agacactatc
     1141 aatatctttt tcttctattt gcttttttaa ttttctagca ttacttttgg ttttgaatgc
     1201 tcctatttga aggtaaatat tactgttact tagttgagtt atagcagcta ttttttcctt
     1261 tgcgatgcta cttttatttt cataatacat acttctagct gcttcttgtc gtgtgatact
     1321 tctcaattct acgaaagcag ttccatcgtc aatcatgcca attccttttg ctgctgcata
     1381 agataagtca attattctat tatctttaaa aggacctcta tcattaactt taacgattac
     1441 agacgctcca ttttttaaat ttctaactct taaatatgag ggaagcggaa gagttttatg
     1501 tgcggcggta tactccaaca tatcataagt ttcaccacat gccgtgcttt taccatcgaa
     1561 atttggtcca taccaagatg caactcctct ctttttatag cctctggcag atcttagaac
     1621 cttatatttt ttgcccctga ccttatatac atatcctttc tctctttcaa ttgtacaatc
     1681 attctgtaag accgatatct ggctagattt tgtctgttgt tttacaacat ttggtacgta
     1741 actacagtta ctagtcaata caataaagat gatgggtaga gtgtatttac taattttttc
     1801 acttctggag ttcattgaat tttggattga atacttctac caacttctat cacagacaat
     1861 gcatacatta cgttcctatt ataggttgta atgacataga aattatgaaa gccaacatag
     1921 tttttttcac tatctccttc taatagtatg acttgtgcag gtgaatcctc gttgaggtta
     1981 gattcaaaag ttatgccatt gtcatttaat gtctcaatgg tgtactgggg tttcagtgtt
     2041 ttcgaagcaa atgtttttag ttgttcatcg tccaagcttg tttgagaaat tatattttcg
     2101 tttctacgcc aaccattttg ctgtaaataa ttggcaatgc tcataatgat gtcactccag
     2161 ttttcaaaaa gatcaatcat gctatcgtta tctccgtcga cagcataagc tctgtagcta
     2221 gaaggtataa attgtgaggc tcccatagcc ccggcgtaag aacctttaat ttcttcaata
     2281 gcaatgcctt gttcacgtgc taggtaaaag agattgatta attgctcttt aaaaaatgca
     2341 cttctaggag gatatcctgt aaccagtgtg tagagagaat ctatgacttt aaaattacct
     2401 tgtattcgac caaagtatgt ttccactcct aggattccta atattatatc taccggcacc
     2461 cctgtttctt gggcagcttt taatagcaat tgttcgtgtt gttttgcaaa tataacacca
     2521 gccgctattc tggcaggtgt tacaaagatt cttctgtatt cattccatgt ttttgtcttt
     2581 tctgctggct tactgataag attgattatc tttggtagat acgtggcttt ttcaaaaact
     2641 gctgtaatgt actgctcatc aaaaccatga tctgttacca attctttaat caattctagc
     2701 ttgattgcct cgttggattc ttttgcttgt aaaacactcg gacagataat caattggatc
     2761 attgataaca agaggatatt aataatcgtt tttttgtagt tcacttttct accaatcttc
     2821 tgtgagtttg gatcgacatc aacattccta aacccagcat tatagttacc attgatgttc
     2881 cccctgcact gattaacggt aggggaatac caacaattgg caagatacca gttgtcattg
     2941 caatatttac aaagacatag acgaaaaaag tcaaactaat gctggatgca agcagtctgg
     3001 agaaaagatt ttgagaattg attgcaataa tgatccccct tccaataata aataagtata
     3061 tgctaagcag agggatgatt cccagaaaac caaactcctc tgcaattaca gagaatataa
     3121 aatctgttgt tctctcaggt aagaaatcca attgtccttg actaccactg agccaacctt
     3181 ttccaaatat tccacctgat ccaatggcaa tttttgattg gattatatga tatccagcac
     3241 ccaaagggtc gttttcaggg tttaaaaagg ttaacacacg atttttctgg aaaggatgca
     3301 tactggacca aatgagtggt aatgaagcaa aaaatgctgc catagcaata gccaagtatc
     3361 tttttctaat gccagataaa aaaatgataa ccaggcctga aaatgcaatg agaaccgctg
     3421 ttccaagatc tggttcaagc aatataagtt cagttggtat cagtactatc aatagtgaaa
     3481 aaataattga ttgattactg ggaggaatct tatctctagc aatatatcca gcaatcatta
     3541 aaggcatcgc aattttcatt aactctgaag gttgaaaatt aattccaatg tttaaccatc
     3601 tagtagcacc atttatagtt tcgccgaata gtaaaaccat cactaacaag gcaacacata
     3661 ataaatacag ccaaaaccct attctttcat aagttaaggg actgaattgg gcaatcgtca
     3721 ccatactaat aatcgcaata attattctaa ttgattggtt gattagcaca tccatattgc
     3781 tgttggatgc actatagatg gcgattaaac tgattataaa aattaacata accgccaata
     3841 ataatgatgg atcaatatga aatttgtaca gaatgccctc agttggatgg ttccaggaga
     3901 tattattttc tacaaccatt acggtttttc tctatctaga aaaacgtcaa gaatttcttt
     3961 tgctataggt gctgctgctg tccgaccact tacaccattt tcaactataa ttgccagagc
     4021 aatttttgga ttatccaatg gggaaaaacc aataaaaagc ccatgatctt taaggttatc
     4081 ggggattttt ttgccttttg ttttttcaat gctgtaaagt tgagaacttc ctgtttttcc
     4141 agcaagagga tagttatttt cgaatatgcc aaaggcagtg ccaccccttt gattaattac
     4201 cgcactcatg ccttgatgga tgatattcca atgatcattg ttaatatctg ttatatcagt
     4261 aagtacactg agtggtacag gttcaatagt ttttgtttga gtgttcttaa tacctttcac
     4321 tagatgaggt tgataggctt tgcctttatt tgcaataatg gtcgttgcta ttgctagctg
     4381 caagggagtt gctagcatat ccccttgtcc aataccgttg ttgacagtat ctcctggata
     4441 ccaattttgg ttttttatag tttttttcca ctcaggatta ggtacaacac cagatttttc
     4501 atagctaata tctattccgg tgagactgcc taaattaaat ttctttaaaa aattactggt
     4561 tgaagttatt cctatgttga ttgcagtttt ataaaaaaaa acatcacaag atgcttggat
     4621 tgcttctttg acattgactg gaccatgctc tctccagtca ttaaaaggtc ttgaataatt
     4681 aggtaaataa aaagcaccat cacatggttc aagtttagtc ggatcaataa atccttgttg
     4741 tagccctgca agggctataa ttggtttgat tgttgaaccg ggaggatatt gcccggcaat
     4801 tgctctattg aataatggct tgtttttatt tgtttgtaag gatctatatt ccgaactact
     4861 gatgcctc
//
LOCUS       DASCTH010000092         1601 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000129267, whole genome shotgun sequence.
ACCESSION   DASCTH010000092 DASCTH010000000
VERSION     DASCTH010000092.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000129267"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="QGH35_01990"
     CDS             complement(<1..1221)
                     /locus_tag="QGH35_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJL81336.1"
                     /translation="MKERKFWGWGYQDQVLSNEEDAAIESLIAAHFSLDEVPTLPIPL
                     AEDIDLPTPRVKIPQTLAKVLSQDHLERLNHTYGKSFPDLARAMLKEFPHPPDLIAFP
                     EDQEDIVNILDWANQNNIAVIPYGGGSSVCGGVETYVGDDYSGVISLDLRNLDQVLEI
                     DTTSRAARIQAGIFGPALEDELRKSDLTLRHFPQSFEHSTLGGWIATRAGGHFATLYT
                     HIDDFVESTTMVTPTGVVESRRLPGSGAGPSPDRIVIGSEGILGVITEAWMRLQNRPI
                     FRSSCSVEFDNYQKALNATRLISQSGLYPANCRLLDANEALLNRAGDGSKHILILSFE
                     SADHELDSWLDRALEIASSEGGSYKKPQEKEPGAHRRGASGTWRNSFIRAPFNRDAAV
                     RRGIIWDTFETSITW"
     gene            complement(1230..>1601)
                     /locus_tag="QGH35_01995"
     CDS             complement(1230..>1601)
                     /locus_tag="QGH35_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012792.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJL81337.1"
                     /translation="QEDDFDRAYDFIGDRLMNEESIIFASSDKPPKLSGFVQLYPSYC
                     SVSTIPILILYDLYVDQEKRGTGVGRILMNQAKDYAKENGYKRLELSTAKDNFIGQSL
                     YQSLGYELDEEFLHYSLENFD"
BASE COUNT          468 a          353 c          277 g          503 t
ORIGIN      
        1 ccatgtgatt gaggtttcaa aagtatccca aattatgcct ctacgaacag ctgcatcacg
       61 attaaaaggt gccctaataa aactgtttct ccatgtgccc gaagctcctc tccgatgtgc
      121 tccgggttcc ttttcttgag gttttttata actccctccc tcagaagagg caatctccaa
      181 agctcgatct aaccaactat caagttcatg gtctgctgat tcgaaagaca atatcaatat
      241 atgttttgat ccatcaccag ctctatttaa taaagcttca tttgcatcca aaagcctgca
      301 attggcagga tacaatcccg attgactgat caggcgtgtt gcattaagtg ctttttgata
      361 attatcaaat tccacggaac aagaagatct aaagatcggt ctattctgaa gcctcatcca
      421 agcttcagtt atcaccccta atatgccttc ggagccaatg actattctat ccggactagg
      481 accggctcca gagcctggca atcgtcttga ttcaacaact ccagttggag ttaccatagt
      541 tgttgattca acaaaatcgt ctatgtgtgt gtaaagcgtt gcaaaatgac ctcctgctct
      601 tgtggctatc caaccaccta aagttgaatg ctcaaaactt tgaggaaaat gccttaaggt
      661 taaatcagac tttcttagtt cgtcttccaa agcaggtcca aatataccag cttggattcg
      721 agctgctctg cttgtcgtat ctatctctaa aacctgatcc agatttctta aatccaaaga
      781 aattacaccg gagtaatcat ctcccacata tgtttcaacg ccaccacaaa cactcgagcc
      841 acccccatag ggtattacag ctatattgtt ttggtttgcc cagtctagaa tattaacaat
      901 atcttcttga tcctctggaa atgcgattaa atcaggtgga tgaggaaatt ctttaagcat
      961 ggctctggca agatctggaa aagacttacc ataggtatga tttaaccttt caagatgatc
     1021 ttgggataaa acttttgcca aagtctgtgg gatttttact ctaggtgtag gtaaatcaat
     1081 atcttcagcc agaggaattg gtaaagttgg cacttcatct agtgagaaat gtgcggctat
     1141 aagactttca attgcagcgt cttcttcatt ggataagact tgatcttgat agccccaacc
     1201 ccaaaatttt ctttctttca ttatttcttc taatcaaaat tctctaggct ataatgcaaa
     1261 aattcttcat ctagttcata acctaatgac tgatataaag attgaccaat aaagttatct
     1321 tttgctgtag atagttctaa tcttttatag ccattttctt ttgcgtaatc ttttgcctga
     1381 ttcatcagta ttcttcccac gcccgttcct ctcttttctt gatccacata gagatcatag
     1441 aggatcagaa tcgggattgt actaaccgaa caataagaag ggtagagctg aacaaaacca
     1501 cttagtttcg gaggtttatc actagatgca aaaataatag attcttcatt catgagtcta
     1561 tcaccgataa agtcataggc tcgatcaaaa tcatcttctt g
//
LOCUS       DASCTH010000093         2321 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000131360, whole genome shotgun sequence.
ACCESSION   DASCTH010000093 DASCTH010000000
VERSION     DASCTH010000093.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000131360"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="QGH35_02000"
     CDS             <1..235
                     /locus_tag="QGH35_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019669869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrogen fixation protein NifS"
                     /protein_id="HJL81338.1"
                     /translation="IRCATVTFLPLKKDFNEVVDTLAKENLMVGVGDFYGVRPLNEMK
                     IDLDPGTIRLSFLHYNTMEEIEHLIAGLKVALG"
     gene            complement(346..756)
                     /locus_tag="QGH35_02005"
     CDS             complement(346..756)
                     /locus_tag="QGH35_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81339.1"
                     /translation="MNIKIKNLLLGMMMVLIGTFFQSNVLAHADHDKDAIIIKMADIM
                     ISLEHVPTSNEKKKIQSVIDSDSSTEQEKIIANAIMNIQHQTTAEDQQKLQKIIDGTA
                     PTSTVSTLASIVQSFSHGISKTDKRKLQTIKFKG"
     gene            complement(778..1998)
                     /locus_tag="QGH35_02010"
     CDS             complement(778..1998)
                     /locus_tag="QGH35_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HJL81340.1"
                     /translation="MFRAITIISLFFHISVVFGHGAVQRESELKDSEIRLFVADNMDG
                     SVIVVDFPDGKIINRVSTSPSIMILAANKDNNYIYAMRGRDTDQDLTSIISTGFDPET
                     GKFRKPHVIRTIETDTPGGPRDDKMATVGGYDAILTEGDAEIVVLMSDLIDGYSELQT
                     KTYKIAAPDHYHYLEGETYLYIGHLFKGFVQVLDRVSGEEVGRIKGCPMLHGMTEDEE
                     SGRLFFSCARDVMVVGTLGEEKAKQISRIPYPENTGRGCAAFLKGEGRVQWCYTEGII
                     PRLYRVDLAQEPYQFTTLSVESSIQQNSTDDGKYLMVLSKQGVLYLYDGQDGDLIKTI
                     KVSEPFLGDWFEDVGKAILPDIESHNGIAYITLTHEGRMAEIDLEQGEVKRYVDLGGF
                     PTRIVLLAKDTLVQ"
     gene            complement(2014..>2321)
                     /locus_tag="QGH35_02015"
     CDS             complement(2014..>2321)
                     /locus_tag="QGH35_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019232121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HJL81341.1"
                     /translation="LVVEPTVESGLEDIIKSMGTGLMVTSLIGHGVNIVNGDYSRGAT
                     GFWVENGEIQYPVSEITIAGNLRDMLNNILLVGKDLNSESSIICGTILLDDLMVAGY"
BASE COUNT          669 a          527 c          402 g          723 t
ORIGIN      
        1 tattcgatgt gcaacagtta ctttcctacc attaaaaaaa gattttaatg aggttgttga
       61 caccttagct aaggaaaatt taatggttgg tgtaggtgac ttttatggag taaggccttt
      121 gaatgaaatg aagatcgatc ttgacccagg tacgattcga ctatcatttt tacactacaa
      181 cacaatggaa gaaattgaac atttaatcgc agggcttaaa gtagcattgg ggtaaattcc
      241 aaaggcattg acaagttaac ttctgcaaat tgaaaagtcc aacatcaatt tacattcaaa
      301 cagtcaagaa atcacaaact gttaatactg gtggatggtt ggttcttaac ccttgaattt
      361 tatggtctgc agttttcttt tatcggtttt tgagattccg tgggaaaagc tctgaacaat
      421 gctggctaag gtactgactg tagaagtcgg ggctgtacca tcaatgatct tctgcaattt
      481 ctgttgatct tcggctgtag tttgatgctg gatgttcatg atggcattgg caatgatttt
      541 ctcctgctcc gtagatgaat cagagtctat caccgattgt atttttttct tttcattaga
      601 agtgggaaca tgctcaagac tgatcataat atctgccatt tttataataa tagcgtcttt
      661 atcatggtct gcgtgagcta acacatttga ctggaaaaaa gttccgatta acaccatcat
      721 catacctaac aataaattct taattttaat attcataacg cttccctgtt acttacatta
      781 ttgaaccaaa gtatccttag caaggagcac tatccttgtt ggaaaaccac ccagatcaac
      841 atatctttta acttctcctt gttccaaatc aatttcagcc attcttccct catgggtcag
      901 agtaatatac gcaataccat tgtgtgattc gatatcgggg agaatcgctt tacccacatc
      961 ttcgaaccaa tccccaagaa atggctctga gaccttaatg gtcttaatta aatccccatc
     1021 ttgaccatca tacaaataca atacgccctg ttttgacaat accatcaggt acttaccatc
     1081 gtcggttgag ttttgttgga ttgaagattc aacagacaaa gtcgtgaatt gataaggctc
     1141 ctgggctaga tccactcgat agagtctggg tatgatgcct tccgtataac accattgcac
     1201 tcgcccttct ccctttaaga atgctgcaca ccctcttcct gtattttcag gatagggaat
     1261 tctagagatc tgttttgctt tttcctcacc caatgttcca acgaccataa catcccgagc
     1321 acaagagaaa aacaaccttc cagattcctc atcttcggtc atgccatgca acattggaca
     1381 gcctttaatc cgtccaactt cttcgcctga tactcggtca agcacttgca caaaaccttt
     1441 aaaaaggtgt ccaatataga ggtaggtctc tccctctaaa taatggtaat gatcaggggc
     1501 tgctatcttg taagtttttg tttgtagctc ggagtaacca tctatcaaat cagacattaa
     1561 caccactatt tctgcatcgc cttcagtcaa aatggcatca taaccgccta ctgtcgccat
     1621 tttatcgtct cttggacctc ccggagtatc ggtctcaatg gttctaataa cgtggggttt
     1681 tctgaacttc cctgtctctg gatcaaatcc tgtacttata atactggtaa gatcttgatc
     1741 agtatcccga ccccgcattg cataaatgta attattgtct ttatttgcag ccagaatcat
     1801 gattgatgga gaggttgata ctctgttgat gattttccca tctggaaaat cgacgacgat
     1861 gactgatccg tccatattat ctgccacaaa gagacgtatt tcactgtcct ttaattccga
     1921 ttctctttgg acagccccat gtccgaaaac cacactgatg tgaaaaaaaa ggcttatgat
     1981 tgttatagct ctaaacactt atcaaactcc taactaataa cccgcgacca tcagatcgtc
     2041 aagcaatata gtgccacaaa ttatggaact ctcactattt aaatccttcc ctaccaataa
     2101 aatattattc aacatatctc ttagatttcc agcaatggta atttcgctaa ccgggtattg
     2161 aatctcaccg ttctctaccc aaaaaccggt agctccacgt gaataatccc cgttcacgat
     2221 attgacgcca tgaccaatta ggctggtaac catcaagcca gttcccatgg atttaattat
     2281 atcctctaag ccgctctcta cagttggctc aaccaccaaa t
//
LOCUS       DASCTH010000094         5553 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000131594, whole genome shotgun sequence.
ACCESSION   DASCTH010000094 DASCTH010000000
VERSION     DASCTH010000094.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000131594"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="QGH35_02020"
     CDS             complement(<1..208)
                     /locus_tag="QGH35_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014617.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81342.1"
                     /translation="MDLQTRELRQIVQLDVESVGFELLDLELSYGPRSTKITVYIDHK
                     NGIDIKDCVTVSKLISMTLNQDIDL"
     gene            complement(259..335)
                     /locus_tag="QGH35_02025"
     tRNA            complement(259..335)
                     /locus_tag="QGH35_02025"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Met,seq:cat)
     gene            complement(367..1800)
                     /locus_tag="QGH35_02030"
     CDS             complement(367..1800)
                     /locus_tag="QGH35_02030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HJL81343.1"
                     /translation="MMLEQIKLLMPEIIIATGICTMVLSSSIKIISSKLISQIIAAVT
                     LMAATASAIFLLPSESQYLFDYQVIIDPLTSGLRISSYVIVLIVIFGTDVNEKINQNE
                     LFLIELFATLGISIICSAANLLIIYLGLEILALSLYTLVASNKHSIESTEAGMKFFIL
                     GAIASAVFLYGISLIYGTYGTLNIHEINSIDSTNTLETQIALGFLLCGIAFKFGAIPF
                     HTWVPDAYQGASTSAALFLSTAPKIAAFALLYRILIDGFMAISETWTIMIQVLGILSL
                     ILGNLIAISQTNMKRLLGYSAIGHVGFIFLGISTGTKNGLESSLFYVLIYVLMMITAF
                     MSLEVLSSKKHKVELISDLKGLNNSHPWFALIMLFVMFSMIGIPPFAGFFAKWSILTS
                     LIDANMIYTAIIAIVMSVVAAFYYLRVVWYIYFEKTELSISQIGSSSLQQITVSCVGL
                     FLLFLGLMPKPLLDFCTKIISSKLLID"
     gene            complement(1808..3310)
                     /locus_tag="QGH35_02035"
     CDS             complement(1808..3310)
                     /locus_tag="QGH35_02035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HJL81344.1"
                     /translation="MNILSTIIFLPIIAALFIILLGRTKVRSGYNLALLVSFVVFALS
                     LYIGIGFDPGLNQFQFIEQVNWISSFNIQYHLGIDGISYPLILLTTLLTPLVILTSKT
                     SPKDKYHQYLASFLILEGLMIGVFTSLDGLLFYIFWESMLIPMFIIIGIWGGNNRVYA
                     TVKFFLYTFLGSIFLLIAIIYLYFQTGGYAITDFHTIPLTMNEQVFIFLAFFLAFAVK
                     IPMWPVHTWLPDAHVEAPTGGSVILAAVLLKMGAYGFLRFSLPITPDASAYFSPFILV
                     LSLIAIVYIGLVAIAQMDMKKLIAYSSIAHMGFVTIGMFVIFGLDNSLIHTEIKSLAM
                     TGAIVQMISHGLISAALFLCIGVMYDRLHTRQISDYGGVINSMPKFAALMVFFGLANA
                     ALPGTSGFVGEFIVILASFQINPWVAVFAATTLIIGAVYTLWLIKRVIYGQTSNQKVE
                     GMTEMTTIEAIPLVILALLVLLLGFWPAPLIEIMSTSVENLVNHIAHSKL"
     gene            complement(3332..5269)
                     /gene="nuoL"
                     /locus_tag="QGH35_02040"
     CDS             complement(3332..5269)
                     /gene="nuoL"
                     /locus_tag="QGH35_02040"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HJL81345.1"
                     /translation="MLELSLIALTSPLIGGLLSGLIGPTLGKWFTNGVTILSVLISFL
                     ISCFILFEIILDPSLIINQDIYTWLSMGNFHFTAGILLDHLTVSMMAVVTFVSLVVHI
                     YTIGYMRHDSGYHRFFCYISLFTFSMLTLVISNNFLQLFFGWEAVGVMSYLLIGFWYT
                     KESAIKANFKAFIVNRVGDMGFLLGIALIFSIIGSLDYQTVFTSNTILLERNIVMTSN
                     ITTSALTLACLLLFIGAMAKSAQIPLHVWLPDSMEGPTPISALIHAATMVTAGVFMIA
                     RMSPLFELSSTALSVVLIIGAITALLTGLLGIVEFDIKRIIAYSTLSQLGYMVVAAGV
                     SAYSASIFHLMTHAFFKALLFLAAGSVIIALHHEQDIRNMGGLRRFMPYTYVATLIGS
                     LSLIGFPGFSGFFSKDALIEAVAHSNVVGAEFAYYAVMIGVFVTALYTFRLLFRVFHG
                     QLRFKGEKIEESPMVIIIPLMLLSIPSMIFAWPTVGDFVYGNFFADSIRQMPSDELFG
                     YNFTDPMSFLIHTLSGTVVYLSLFGVLTAWFFYIYKPDLPEKFLQFLKPIHTLLWNKY
                     YFDHFYEKVIAKSVASLGSFFWNFGDVKIIDDTLVNGTANTIAKLSAFTRRIQTGYIY
                     HYALMMMIGLSFFLAWAIIRS"
     gene            complement(5272..5550)
                     /gene="nuoK"
                     /locus_tag="QGH35_02045"
     CDS             complement(5272..5550)
                     /gene="nuoK"
                     /locus_tag="QGH35_02045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HJL81346.1"
                     /translation="MAALLFAIGAMGIIINKKNIIIVMMSIELMLLAVNFNFIAFSYF
                     SNDLTGQIFVFFMLAIAAAEAAIGLAILVTLYRNKNTISVREFKQLKE"
BASE COUNT         2001 a         1018 c          977 g         1557 t
ORIGIN      
        1 cgagatctat atcctgatta agagtcatgg aaatcaactt actgactgtt acacagtctt
       61 taatatctat gccattctta tggtctatat acactgtaat ttttgtagat cttggcccat
      121 aactcaattc caaatctaat agttcaaagc caaccgattc aacatctaat tggactattt
      181 gtcttagctc tcttgtttgt aaatccatat ttagtctcat aaaaataaag ccccagatac
      241 ggggcctgac caaaaaattg gtagcggggg taggatttga acctacgacc ttcgggttat
      301 gagcccgacg agctgccaga ctgctccacc ccgcatctgt aggtgaatga tactaactgt
      361 ttatagctaa tctatcaata gcttagatga aataatttta gtacaaaaat ccaataaagg
      421 tttgggcatt agtccaagaa aaagaaggaa caaacccaca caagacactg tgatttgttg
      481 taaagagctt gatccaattt gcgatatgct gagttctgtt ttttcaaagt agatatacca
      541 aacaactctg aggtaataaa aagcagccac gacagacatg acaatggcta tgattgcggt
      601 ataaatcata ttggcatcaa tcagtgatgt caaaatactc cactttgcaa aaaatcctgc
      661 aaaaggtgga attccgatca tactaaacat aacaaatagc ataatcaaag caaaccatgg
      721 gtgtgagtta ttgagacctt ttagatcaga gattaattct actttgtgtt tttttgaaga
      781 taatacttct aaagacataa atgcagtaat catcatcagt acataaatca aaacgtaaaa
      841 taatgaactc tccagaccat tttttgttcc cgttgatatg cctaaaaaaa taaacccaac
      901 atgaccaatg gctgaataac ccaataacct tttcatatta gtctggctga ttgcaattaa
      961 attacccaga atcaatgaga gaatacctag aacttggatc attatggtcc atgtttctga
     1021 aatggccatg aatccatcaa tcaaaattct atacaacagt gcgaatgctg caattttagg
     1081 tgctgtggat aaaaataagg cagctgatgt tgaggcaccc tgatacgcat caggtaccca
     1141 ggtatgaaaa ggaatagcac caaatttaaa ggcaatacca cataacagaa aacctagagc
     1201 aatttgtgtt tcaagtgtat tggttgaatc aatgctattg atttcatgaa tattgagagt
     1261 tccataagta ccatagatca gagaaattcc atataaaaat actgccgatg caatcgcacc
     1321 taatataaag aacttcatgc ctgcttctgt tgactcaata gaatgtttat tagaagccac
     1381 cagtgtatag agggaaagtg ctaagatttc taaacctaaa tatatgatta ataagttggc
     1441 agcagagcat atgattgaaa tgccaagtgt tgcaaacagt tctattaaga agagttcgtt
     1501 ttggttgatt ttttcattaa catcagttcc aaatattaca ataagaacaa taacatatga
     1561 ggatatcctc aagccagatg ttaaaggatc aatgataact tggtagtcaa ataagtattg
     1621 ggattcactt ggaagtagaa aaattgcact tgcagttgca gccatcagag ttacggcagc
     1681 gatgatttgg gatatcaatt tacttgaaat gatttttata ctactactta gaaccattgt
     1741 gcaaattcca gtggctataa taatttcagg catcagtaat tttatttgct ctaacatcat
     1801 ctcttttcta caactttgaa tgggctatat gattaactaa attttctaca ctggttgaca
     1861 ttatctcaat caatggtgca ggccaaaaac ccaataataa aactaatagt gcaagaatca
     1921 ccagaggtat ggcttcaatt gttgtcattt ctgtcattcc ttcaaccttt tgattactgg
     1981 tttgaccata gatcactctt tttatcagcc acaaagtata aactgctcct ataatcaatg
     2041 tagttgctgc aaaaacagca acccatggat taatttgaaa gcttgcaaga atgacaataa
     2101 attcgccaac aaaacctgaa gttccaggta gagcagcatt ggccagccca aaaaatacca
     2161 ttaaggcagc aaattttggc atcgaattga tcacccctcc ataatccgaa atctgtctag
     2221 tatgtaaccg atcgtacatg actccaatac atagaaatag tgctgctgaa attaatccgt
     2281 gagaaatcat ttgaacaatt gcaccggtca ttgccagact ttttatttct gtatgtatca
     2341 atgaattatc taaaccgaat atgacaaaca taccaatagt cacaaaaccc atatgtgcaa
     2401 tagaggaata ggctatgagc tttttcatat ccatttgtgc gatcgctaca agaccgatat
     2461 agacgattgc aattaaagat aaaacaagaa tgaatggtga aaaatatgca gatgcatctg
     2521 gtgtaatcgg cagactaaac cttaggaatc cataggcacc catttttagt aaaacggctg
     2581 ctaaaatcac ggatccccca gtaggagctt caacatgggc atcgggtaac catgtatgga
     2641 ctggccacat tggaattttt actgcaaaag caagaaaaaa tgctagaaat ataaatactt
     2701 gttcattcat ggttaacggt attgtgtgaa aatccgttat tgcataacca cccgtttgaa
     2761 aataaagata aattattgca atcagcaaaa agattgaacc aagaaatgta tatagaaaga
     2821 attttacggt tgcgtaaaca cggttgttgc ctccccatat accaatgata atgaacatag
     2881 gaatgagcat ggattcccaa aaaatataaa ataataatcc atctaaggag gtaaatactc
     2941 cgatcatcaa cccttctaga attaaaaaag aggcaagata ttgatggtat ttatctttgg
     3001 gagaggtttt tgaagttaag ataaccagtg gcgtcaatag agtggtcaaa agaattaaag
     3061 ggtatgatat tccatcaatg cccaaatggt attggatatt gaaactactg atccaattaa
     3121 cctgttcaat gaattgaaat tgattgagac caggatcaaa accaatgccg atataaagag
     3181 aaagagcaaa taccacaaac gacacgagca aggctaaatt ataaccgctc ctaactttag
     3241 ttctacccaa tagaataatg aaaagtgcag caataatggg taagaatatg attgtggaaa
     3301 gtatgttcat ttaatcatct tctagatttc cttatgatct tatgattgcc caagcgagga
     3361 aaaaagaaag tccaatcatc atcatcaatg catagtggta tatataaccc gtttggattc
     3421 ttcttgtgaa tgctgacaat ttagcaattg tgttggctgt gccattgacc aaagtatcat
     3481 caattatttt aacatcgcca aaattccaaa aaaatgaacc taggctggca acagattttg
     3541 caattacttt ttcgtagaaa tgatcaaaat agtatttatt ccataatagc gtgtgtatgg
     3601 gtttcaagaa ttgtaaaaat ttctcaggta aatccggttt gtaaatatag aaaaaccaag
     3661 cagtcaatac tccaaaaaga gaaagataaa ccacagtgcc tgaaagggtg tgaattaaga
     3721 agctcattgg gtcagtgaaa ttataaccaa ataattcatc actcggcatt tgacgaatgg
     3781 aatcagcaaa gaaattaccg taaacaaaat cacctacagt tggccaagca aatatcatcg
     3841 aagggattga gagcaacatc aatggaataa taatgaccat tggcgattct tcaatctttt
     3901 cacctttgaa acgtaattga ccatgaaaaa ctctaaataa aagtctaaat gtataaaggg
     3961 cggtgacaaa aactccaatc ataactgcat aataggcaaa ttcagcacct actacatttg
     4021 aatgagcaac agcctctatc aaagcatctt ttgagaaaaa tcctgaaaaa ccaggaaaac
     4081 caattaatga tagcgatcca attaaagtag ccacatatgt atatggcatg aaacgcctca
     4141 agcctcccat atttcgaata tcctgttcat ggtgcaaggc tatgatcacc gatccggctg
     4201 ctaaaaataa taaagcttta aagaaagcat gagtcatgag atgaaatata ctggcagaat
     4261 aggcagacac acctgccgca actaccatat atcccagttg ggataatgtt gaataggcaa
     4321 taattctttt gatatcaaat tccacaattc ctaataatcc agtcaacaat gccgttattg
     4381 ctcctatgat tagaacaaca gataacgcag tggatgataa ttcaaataaa ggagacattc
     4441 tggcaatcat aaaaacgcca gctgtaacca tggttgctgc atgaatcaat gctgagatcg
     4501 gtgtcggtcc ttccattgaa tcaggtaacc atacgtgcag tggtatttgg gcagattttg
     4561 ccattgcacc tataaataat aacaaacatg ctagtgttaa tgctgaggtg gttatgtttg
     4621 atgtcatcac aatgtttctt tccaataaga ttgtattgga ggtaaatact gtttggtaat
     4681 ccagggatcc aatgattgaa aatatcaagg caattccaag caaaaaaccc atgtcgccta
     4741 ctctgtttac aataaaggcc ttaaagtttg ctttaatcgc actttctttc gtataccaga
     4801 atccaattaa tagataagac ataacgccga ctgcttccca accaaagaat aattgcagaa
     4861 aattattaga tatcactaaa gtcagcattg agaatgtaaa taatgagatg tagcagaaaa
     4921 aacgatgata tccagaatcg tgacgcatat accctatggt gtaaatgtga actaccagtg
     4981 aaacaaaagt aacgaccgcc atcatactga ctgttaaatg atccaatagg attcctgcag
     5041 taaaatggaa attacccata gaaagccaag tataaatatc ctgattgata atcaaacttg
     5101 gatctaaaat aatttcaaac aatatgaaac aggaaatcaa aaaagagatg aggacgctca
     5161 gaatcgttac cccattggta aaccatttgc ctaatgtagg accaatcaat cctgataaca
     5221 ggccccctat caggggcgac gtgagagcaa ttaaactcag ttcaagcatc cctactcctt
     5281 taattgttta aattctcgaa cactgatggt gtttttattt ctataaagag tgacgagaat
     5341 cgctaaacca attgctgcct cagctgctgc aattgctaac ataaaaaata caaatatttg
     5401 accggtgaga tcattgctga aataggaaaa agctataaag ttaaaattta ctgccaatag
     5461 cattaattct atggacatca ttacaatgat gatgttcttt ttattgataa tgatacccat
     5521 tgcaccaatt gcgaaaagca gagcagccaa agt
//
LOCUS       DASCTH010000095         2952 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000132249, whole genome shotgun sequence.
ACCESSION   DASCTH010000095 DASCTH010000000
VERSION     DASCTH010000095.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000132249"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="QGH35_02050"
     CDS             <1..236
                     /locus_tag="QGH35_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81347.1"
                     /translation="ELKNDKKEGTWEFYYDNGQLQAVEKYSGGRPVGIWKYYGEDGRF
                     LKRVNQLMGDHSLGTIAPNGVDNLDCKMDYGFC"
     gene            complement(355..1287)
                     /locus_tag="QGH35_02055"
     CDS             complement(355..1287)
                     /locus_tag="QGH35_02055"
                     /inference="COORDINATES: protein motif:HMM:NF025587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HJL81348.1"
                     /translation="MSDLIKTVSFDLWDKDPEQFAEKLGQNHKSTGFCAIKNHPINKE
                     LVDSCVAMFADFFAQSEELKMKYFDEDMGGARGYTPYKIETPQGGEHADLKEFWQMGR
                     ELPENHPYKQFMFDNVYVSEIPEFKDKMQQLFKAFDLFAKQLMQAIAIYLDLNQFYFD
                     SAIDNGNSILRVIHYPPIESEDPGERSGAHKDINLFTLLIGGSEPGLEILLNGQWIPV
                     HIERDVLLCNVEEMLERFTNNRIPALIHRVTKGPTKITDSARFAIPFFVHPNPDWLIE
                     TLPSCIDAEHPDLYPESILTDDFLQQRLYEIKLL"
     gene            complement(1444..2085)
                     /locus_tag="QGH35_02060"
     CDS             complement(1444..2085)
                     /locus_tag="QGH35_02060"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HJL81349.1"
                     /translation="MEAKRAELVEILRHQGIKNFKVLDCINKIPRHLYVDESYLHLSY
                     RNHPLPIGNEQTISQPYIVALMTETLLGDSNTEIDGLQKVLEIGTGSGYQTAVLASLY
                     ERVYSIERIHALMNKAKKINQSLGLENVIYQNSDGWLGWPEEAPFDGIMVTATCESVP
                     DILIEQLKVGANLIAPIGQKSSQILYRINKTGSGYNQDPLCAVSFVPMLKGFC"
     gene            complement(2108..2692)
                     /locus_tag="QGH35_02065"
     CDS             complement(2108..2692)
                     /locus_tag="QGH35_02065"
                     /inference="COORDINATES: protein motif:HMM:NF025033.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJL81350.1"
                     /translation="MSLNIWYKNVLKNPAEIAALFPCSAETGMLMTAHLEGMDEGYVL
                     ELGSGMGALTECILQRQVPEEKLILIDKNKDFAGYLSQKYKDANVIASDALDAFDLLQ
                     KMQIYEVTHIVCSLPLIIIPQDRQLALLDAAFKLLQPQGCFSTLTYFVRCPISQKILD
                     QYNKRSKLFGFTWKNLPPAYIYQIKEKQNIGSVI"
BASE COUNT          843 a          561 c          598 g          950 t
ORIGIN      
        1 gagagcttaa gaatgataag aaagagggca catgggaatt ctattacgat aacgggcagt
       61 tacaagcagt agagaagtat agtggtggca gacctgtggg tatttggaaa tactatggag
      121 aagacggtag attcttaaag agggtcaatc aactgatggg tgatcactcg ttaggaacaa
      181 tcgcacctaa tggcgtagat aacttggatt gcaaaatgga ctatgggttc tgctagacta
      241 ggtttgaata gtctttgggc atgaaatttt tttttgctaa caccaattca ccacaaacct
      301 cacctaacaa caaatgacgg agagactttc tcgccggtac cgataaaact gtctttataa
      361 aagttttatc tcgtataaac gctgttgaag aaaatcatcc gttagaatgc tttccggata
      421 gagatcaggg tgttctgcat caatacaaga tggcaaggtc tcgatgagcc agtctggatt
      481 ggggtgaaca aagaatggta tggcaaagcg agcactgtca gttatttttg taggaccctt
      541 cgtgactcga tgtattaatg ctggtatacg attatttgtg aatctttcta gcatttcttc
      601 aacgttacaa agaagcacat ctcgctctat gtgtactggt atccattgac cattgagcag
      661 aatctctaac cctggttcgc taccacctat aagaagagta aataaattta tatctttgtg
      721 ggcaccagat cgttctccag gatcttccga ttctatggga ggataatgaa taactctgag
      781 gatgctattg ccgttatcta tggctgaatc aaaataaaat tgattcagat ccagatatat
      841 agctatggct tgcatgagtt gcttggcaaa cagatcaaat gccttgaata actgttgcat
      901 cttatctttg aattcaggga tttcagagac atagacatta tcaaacataa attgcttata
      961 aggatgattc tcaggcagct ccctgcccat ttgccagaac tctttcaaat cagcatgttc
     1021 tcctccttgt ggggtttcta ttttataggg tgtataacct ctagctcctc ccatatcttc
     1081 gtcaaaatac ttcattttca gttcttcaga ttgggcaaag aaatcagcga acatcgcaac
     1141 acaactatcc actagttcct tattgatggg gtgattttta atagcgcaga atcctgtgga
     1201 tttatggttt tggcctagtt tctctgcaaa ttgttctgga tctttatccc agagatcaaa
     1261 actgactgtt ttaattaagt ccgacatgtt tgcctcctta taatttagtg gaatttgatt
     1321 cctcagggta gacttcagtc ggttcaaaca cctttttttc attatattgc agtttaacgc
     1381 tttgggcgtt accgatgcct tgatctatat caattatttg atatgaatct caccaagagt
     1441 tttttagcaa aaacctttga gcatagggac aaaggataca gcacagagtg gatcctgatt
     1501 ataaccactc cccgttttgt taattcgata gagtatttgt gaagattttt ggccaatggg
     1561 agcgattaaa tttgcaccga ctttaagctg ttctataagt atgtcaggga ctgattcaca
     1621 ggttgctgtt accatgatgc catcgaaggg agcttcttca ggccagccca accacccatc
     1681 tgaattctgg tagataacat tctctaaacc caaggattga ttgatttttt tggctttgtt
     1741 catcagggca tgaatcctct cgatgctgta cactctttca tataatgagg ctagtaccgc
     1801 tgtttgatat ccagaaccgg taccaatttc tagtactttc tgaagaccgt caatttctgt
     1861 attgctatct ccaagcaatg tttctgtcat cagggcaacg atataaggct gggagattgt
     1921 ttgctcatta ccgataggta atggatggtt tcggtaagat agatgcaagt aggactcatc
     1981 tacataaaga tgccttggga ttttattaat gcagtcgagt accttaaaat ttttgatgcc
     2041 ttgatgtctt aggatctcta cgagttctgc tcttttagcc tccataaata aaagtttggg
     2101 ttagctatta aatgacactt ccaatgttct gtttttcttt tatttgataa atgtatgctg
     2161 gaggcaaatt tttccatgtg aatccaaata gcttagaacg tttattgtat tgatctaaaa
     2221 tcttttgtga aattgggcaa cgaacaaaat aagtaagggt ggagaaacaa ccctgtggtt
     2281 gtaatagttt gaatgcggca tctaagagag ctaactgacg gtcttgcggt atgatgatta
     2341 atggcaggct acaaacaatg tgtgtaacct cgtagatctg catcttttga agtaaatcaa
     2401 atgcatctaa ggcatcgctc gcgatgacat tagcatcttt gtatttttga ctgaggtaac
     2461 cggcaaagtc tttattctta tcaatcagta ttaatttctc ttcagggact tgtctttgca
     2521 ggatacattc tgtgagagct cccatgcccg aaccgagttc caaaacataa ccttcatcca
     2581 tgccttcaag gtgtgctgtc atgagcatgc ctgtttcagc actacaagga aacaaggctg
     2641 cgatttcagc tgggttcttc aacacatttt tgtaccaaat atttaaggac ataaatctaa
     2701 gatcttcatc atttgactaa ttatttaaaa actatatcac aactaccaat cctatccatg
     2761 atatgggcaa ggctattgag ctcaaaagat tattatttta atttcttttg cttgagttca
     2821 tccgcaacaa aactgattga ccaattatgg cctaaacgtc tgcctttgta ggtatctggt
     2881 aatagagtta cttcgcttcc ctttaggtca atgacctgag gatcagattc gagattattg
     2941 aataccccat cg
//
LOCUS       DASCTH010000096         3693 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000134437, whole genome shotgun sequence.
ACCESSION   DASCTH010000096 DASCTH010000000
VERSION     DASCTH010000096.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000134437"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="QGH35_02070"
     CDS             complement(<1..940)
                     /locus_tag="QGH35_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81351.1"
                     /translation="MNHTKEKMFYGWYVAIGCTLIYFFTNAMTLFVPETLFPRLMEEF
                     GSNEAEISKTVIITLLFASLFAPLAGILIDKFGPLRIMRIGIIIMALSFSFYGLADSV
                     SALYRLHFFLAIGLVFCGLVVNVIIVSNWFVKHRGKVVGMLVAGSSLGGAILPLLISP
                     IVNHPDWGWRWGYGLLSIFFWLFAVIPAYTFIKETPESMGLSPDGLDQSIPVQTIAGV
                     SLWTAIKSRALWCLGIGSACLWFSIQGLNSQITIFFEQEANFSPQQAVFLFSILFWFS
                     FIGKFTFGAISDKIPKRKVLLLASIILFIGSLLLFDI"
     gene            1043..2383
                     /locus_tag="QGH35_02075"
     CDS             1043..2383
                     /locus_tag="QGH35_02075"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020533669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HJL81352.1"
                     /translation="MKNDAFAIINVNIVNEDLISESDVIIRDGRISKIGSNLSVPNDL
                     EVIDAKGKYLLPGLIDDQVHFREPGLTHKGGIASESAAAVAGGVTSFMDMPNVDPATT
                     TRALLEEKYALASGKAYANYAFYLGATNSNIDEIKQLKIGDACGLKVFMGASTGNLLV
                     NDSTALDNIFAECPILIATHCEDTPTIERNLAKAMNQYPAGKIPVSEHPNIRSVEACY
                     KSSFKAVQYAKKHDSKLHILHLTTEKEMGLFSAGDCITKKITAEVCVHHLYFDDSYYD
                     LMGSKIVCNPSIKTKKDCHSLRAAVRSGKIDVIATDHAPHTLEEKEKPYPDHPAGLPL
                     IQHSLLILLDLYLQKQFSLETIVDKTSHALAKIYGIKDRGFIREGYWADLVLVDCSKT
                     TTVTPKNIFYDCGWSPFEGHVFKSLIDKTFVNGKLVYNDGQLAKKSTGQRIEFV"
     gene            2564..>3693
                     /locus_tag="QGH35_02080"
     CDS             2564..>3693
                     /locus_tag="QGH35_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJL81353.1"
                     /translation="MQPSNTKDPQYYHKVVDCQWGCPAHTNIPEYIRLIAQGQYTEAY
                     LLNRESNVFPGILGRVCDRPCEPVCRRARVDVDHLRWEEPVAICRLKRVAGDLKDNFK
                     DFLPPVPGKKNGKKIVCIGAGCASLTVANDLLPLGYEIDVFESLPKMGGLIRSNIPKF
                     RLPPNIIDEEFDVIVERGINLHLNHPIESMKELLEEHYDAIFVGTGAPHGRDLDLPGR
                     HDSDQIHIGIAWLESVAFEHIDQVGKRVIILGGGNTAMDCCRTSLRLGGKDVKVIYRG
                     SRDRMKSSDWELEPALEENVELILNHTPEEYLVEDGQLVGVKFAHLQWSTDSDGRLSS
                     EKIDEIIIPCDTVILAIGQENAFPWVERDIGLEFDQWEVPIV"
BASE COUNT         1227 a          669 c          750 g         1047 t
ORIGIN      
        1 cgatatcaaa tagcaataaa gagcctataa ataggattat tgaggctagt agaagcactt
       61 ttctttttgg aattttgtcg ctgattgctc caaatgtaaa ttttccaatg aagctaaacc
      121 aaaaaaggat tgaaaataag aataccgctt gttgaggtga aaaatttgct tcttgttcaa
      181 aaaaaatagt gatctgactg ttaagacctt gtatggaaaa ccacagacaa gcagaaccaa
      241 tcccaagaca ccataaggct ctggatttta tggcggtcca caagctaaca cctgctattg
      301 tctgtaccgg tatagattga tccaaaccgt ctggtgagag tcccatgctt tcaggagttt
      361 cttttatgaa cgtataagcg ggaatgactg caaataacca aaagaaaata ctcagtaatc
      421 catatcccca tcgccatccc caatctgggt gattgacaat tggggaaatc aataagggta
      481 aaatcgctcc accaaggctt gatccagcta ctaacattcc cactactttt cctcggtgtt
      541 tcacgaacca attagaaaca ataattacat taaccaccaa tccacagaaa accaagccaa
      601 tcgctagaaa aaaatgcaac cgatataaag ctgacaccga gtcagctaaa ccataaaaac
      661 tgaaggacag agccatgata ataataccta tcctcatgat tcttaatggg ccgaatttat
      721 cgattagaat accagccaaa ggagcaaaca aagatgcaaa aagtaaggta atgatcactg
      781 ttttactgat ttctgcttcg ttagatccaa actcctccat gagtcttgga aatagtgttt
      841 ccgggacaaa aagtgtcatg gcattggtaa agaaataaat tagagtacaa ccaatggcaa
      901 cataccatcc atagaacatt ttttctttgg tgtgattcat cttgatcttt ccttggactt
      961 gttcaataac gacacttatc acctagatga taacatcgat ctaagtgtcc ttgatgttta
     1021 aacgattgct ataatggtta ctatgaaaaa tgatgctttt gcaataatca atgtcaacat
     1081 tgtcaatgaa gatttgattt cagaaagtga tgtaatcatt agagacggcc ggataagcaa
     1141 aatcggatcg aatcttagtg tccccaacga cttagaagtc attgatgcga aaggtaaata
     1201 tcttttacca gggctgattg atgatcaagt tcatttccgt gaaccaggtt taacgcataa
     1261 agggggaata gcatcagaat cggccgcagc agtcgctggt ggcgtgacca gttttatgga
     1321 tatgcccaac gttgatcctg caacaacaac cagagctctc ttggaagaga aatatgcatt
     1381 ggcgtctggt aaagcttatg ccaattatgc cttttatcta ggtgctacta attccaatat
     1441 tgatgaaatt aaacaattaa agatcggaga tgcatgtgga ctaaaagtat tcatgggtgc
     1501 atccacagga aatttactgg tcaatgattc aaccgctcta gataatattt ttgccgaatg
     1561 cccaatattg atcgcaacac attgtgaaga tactccaacc attgaaagaa atttagcgaa
     1621 agcaatgaat caatacccag ctggcaaaat accagtctct gaacacccca atatccgctc
     1681 tgttgaagcg tgttataagt cttcgttcaa agcagtgcaa tacgctaaaa agcatgatag
     1741 taaattacat attctgcatc tcaccacgga aaaagaaatg ggtttatttt cggcaggaga
     1801 ttgtataaca aaaaaaataa cggccgaagt gtgtgttcac catttgtact ttgacgatag
     1861 ttattacgat ctaatgggct ctaagatcgt gtgtaatcct tcaattaaaa ccaaaaaaga
     1921 ttgtcatagt ctaagagcgg cagtgcggtc tggaaaaatt gacgtcatcg caacagacca
     1981 tgctcctcat acactggaag aaaaagaaaa accatatcct gaccatcccg ctggattgcc
     2041 tctcattcaa cattctttgc ttattctttt agatttgtat cttcaaaaac agttcagctt
     2101 agaaacaatt gttgataaaa ccagtcatgc attggcaaaa atttatggaa taaaggatcg
     2161 cggatttatt agagaaggat actgggctga tctggttcta gtcgattgta gcaaaacaac
     2221 aaccgttaca cccaaaaaca ttttttatga ttgcggctgg tcaccgtttg aaggacatgt
     2281 atttaagtca ttgatagata aaacatttgt taacggaaaa ttggtttata acgatggaca
     2341 attggccaag aaatccactg gacaaagaat tgaatttgtt tgatgttggt aaaccttcta
     2401 aaacggtaaa ataacgtttt gtttctacac gtgatatgtt aattttcaac tattctttga
     2461 attggtgttg aaactagaaa caaaaaaaac tggaaattat tagggttaaa tttcagaaac
     2521 acaatagctt ctatagaata acgtcataaa gttgtcataa atcatgcaac ctagcaacac
     2581 aaaagatcct cagtattacc ataaagtggt tgattgccag tggggttgtc cagcacatac
     2641 aaacattcct gaatacataa gattgattgc acaagggcaa tatacagaag cctatttatt
     2701 aaatcgcgaa tccaatgttt ttcctggaat attgggccga gtttgtgata gaccttgtga
     2761 acctgtttgc agaagagcca gagtggatgt agaccattta agatgggaag agccagtagc
     2821 aatttgtcga ttaaaacgtg tggcggggga cctcaaagat aattttaaag acttcctacc
     2881 acccgtgccg ggtaagaaaa acggtaaaaa aattgtatgt attggagcag gttgtgcttc
     2941 attaaccgtt gccaatgact tactcccttt aggctatgag atagacgtgt ttgagtctct
     3001 acctaaaatg ggaggcttaa tcaggagtaa tattccaaaa tttaggctgc caccaaatat
     3061 tatcgatgaa gaatttgatg tcattgttga gcgtggaatc aatctccatt taaaccatcc
     3121 tatagaaagt atgaaagagt tactggagga acactatgat gccatttttg ttggtaccgg
     3181 tgctccacat ggcagagatt tagatttacc tggtagacat gatagtgatc aaatccatat
     3241 tggcatagct tggttagaat cagtagcttt tgaacatata gatcaggtgg gtaaaagggt
     3301 cattatttta ggcggcggca atactgcgat ggattgttgt agaacctctc tcaggttagg
     3361 tggcaaagac gtcaaagtga tttatcgtgg cagtagagat cgaatgaaat cttcggattg
     3421 ggaattagaa cccgcgttgg aagaaaatgt cgagttaatc ttgaatcata ccccggaaga
     3481 gtatttggtt gaagatggtc aattagtggg tgtgaaattt gcccatcttc aatggtctac
     3541 tgattctgat ggcaggttga gctctgaaaa aatagatgag attatcattc cttgtgacac
     3601 agttatcctt gccattggcc aagaaaatgc attcccatgg gtagaaagag atatcggttt
     3661 agagtttgat caatgggaag ttccaattgt taa
//
LOCUS       DASCTH010000097         2239 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000135922, whole genome shotgun sequence.
ACCESSION   DASCTH010000097 DASCTH010000000
VERSION     DASCTH010000097.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000135922"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="QGH35_02085"
     CDS             <1..1414
                     /locus_tag="QGH35_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81354.1"
                     /translation="IIDRIEIIKAPRTSLYGSNAIGGVINIITNQTFQSGYQIGYKMG
                     SDQTSMLDLSGQFTAEKLQGGLHFNQYRTDGFPAKQGSDINSGHNNDSLNAFLNFTED
                     DWFIHSNIWFSSGTTEYLDFFQSPVSQDFMNLSGSIDINKTMSELWTSNLNIGFTTDD
                     LDQNDLVDFNDSRKLFFEWQNNFQVNKNHQIVMGTYWADESFEANNYGLIVDTNKQSK
                     ALYLEDIIALGRHRFLAAARLSNKEEIDDKATWNLEYGYTINPMLRLFINSGTAIRNP
                     SGFDLYGFGGNQELTPEESKNVGLGFVLTPTNNISIEFSAFNNQIEDLIAFDYSDFKL
                     YNIEQTRTRGIESRLQWLVNNWNISFNTKLQNPKNNSTGEVLLRRAKKTLSLGLRRSF
                     GALDVNLNLFESGSRTDFGGVKLDGYTLANMTLQYHFNEAWLMRTVVQNITDETYVLA
                     DGYNTAKRKVFLEVVYQPEY"
     gene            1477..2028
                     /locus_tag="QGH35_02090"
     CDS             1477..2028
                     /locus_tag="QGH35_02090"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HJL81355.1"
                     /translation="MGNRLTKIFTRKGDDGLTDLGNNERVPKYNQQVEVFGTLDELNA
                     AIGIVITCTEIPLTIESFLAEVQQNIFNICGEIAAPQFKSIDQNNVVAVENFINDLNE
                     TLPPLKDFVLPGGNSHSAHCHLARAICRRAERELCKLADEKEINQESFKYLNRLSDAL
                     FVIARTFAKQDNGEEIIWKHPKV"
     gene            2049..>2239
                     /locus_tag="QGH35_02095"
     CDS             2049..>2239
                     /locus_tag="QGH35_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylene tetrahydromethanopterin
                     reductase"
                     /protein_id="HJL81356.1"
                     /translation="MDIEINLEPDLHPDKFAELAVAAEKYGVRAIWSSSYHQNWDPFL
                     SLVPAIQKTSKILLGPLAI"
BASE COUNT          761 a          388 c          429 g          661 t
ORIGIN      
        1 tatcattgac cgaatagaaa ttatcaaagc cccaagaacc agtttgtacg gatcaaatgc
       61 cattggtgga gtcattaata tcattaccaa tcaaacattc cagtctggtt atcaaatcgg
      121 ttataaaatg ggttcagacc aaaccagtat gttggatctt agtggtcaat tcacagctga
      181 gaaacttcaa ggaggattac acttcaatca atatagaact gatggctttc ctgcaaaaca
      241 gggaagtgat attaatagcg gtcataataa tgattccctt aatgcttttt taaatttcac
      301 cgaagacgac tggttcatcc attctaatat ttggttcagt tcaggtacca cagaatatct
      361 agacttcttt caaagccctg taagtcaaga ttttatgaat ctgtcaggtt ctattgatat
      421 caataaaaca atgagtgaac tatggacatc aaatttgaat attggcttca cgacggatga
      481 tttagatcaa aatgatttgg ttgatttcaa tgacagtaga aaactatttt ttgagtggca
      541 aaacaatttt caagtcaata aaaatcatca aattgtaatg ggtacatatt gggcagatga
      601 atcatttgaa gcaaacaatt acggactgat agtcgatacc aacaaacaat caaaagcctt
      661 gtatcttgaa gatataattg ctctcggcag acaccgattt cttgcggcag ccagactaag
      721 taacaaagaa gaaattgacg ataaagcaac ttggaatctc gaatatggct atacgattaa
      781 cccaatgtta agacttttta tcaattctgg aacagccata agaaaccctt ccggctttga
      841 tctatatggt tttggtggaa atcaggaact cactccagag gaatctaaga atgttggatt
      901 aggttttgtt ctaactccaa ccaataatat aagcattgaa ttctcagctt ttaacaatca
      961 aatagaagat ctcattgctt ttgattattc tgacttcaaa ctctataaca tagaacaaac
     1021 aagaactcgg ggtattgaga gtcgtctcca atggctggtg aataattgga atatttcttt
     1081 caatacaaaa ttgcaaaatc caaaaaataa ttcgaccggt gaagttcttt taaggagagc
     1141 taaaaaaaca ctctccctag gtctaaggcg ctcctttggt gcgctggatg ttaatctgaa
     1201 tctttttgag agtggctcta gaacagattt tggtggagtt aaacttgatg gttatactct
     1261 ggctaatatg accttacaat atcatttcaa tgaagcctgg ttaatgagga cagtagtcca
     1321 aaatatcaca gatgagacct acgtattggc tgatggttat aacacggcga aaagaaaagt
     1381 tttcctagaa gttgtttatc agcctgagta ttaattttat tgcctcaatt gcattataaa
     1441 agataagata aggacgtagc attacaagga acaggtgtgg gcaatcggct aacaaaaatt
     1501 tttaccagaa aaggcgacga tggtttaaca gatcttggta acaatgagcg cgttccaaaa
     1561 tacaatcaac aagttgaagt ctttggcaca ctggatgagt taaatgctgc cattggtatt
     1621 gtcatcactt gcactgaaat acccttaacc atagaatcgt ttcttgctga agttcagcaa
     1681 aatattttca atatttgtgg agaaatagca gctcctcaat ttaaaagtat tgatcaaaac
     1741 aatgttgtgg ctgtcgaaaa ttttattaat gacctcaatg aaacactgcc tcccttaaaa
     1801 gattttgttc tacctggcgg gaacagccat tctgcacatt gccatctagc aagggcaata
     1861 tgtcgacgag ctgagagaga gttgtgcaaa cttgcagatg aaaaagaaat taatcaagag
     1921 tcgttcaaat acctaaatag attatcagat gctttatttg tcattgccag gaccttcgca
     1981 aaacaagaca atggggaaga gatcatttgg aagcacccaa aggtttaaat atttagagct
     2041 agattattat ggatatagaa ataaacttag agccagattt gcatcctgat aagtttgctg
     2101 aacttgccgt agctgcagaa aaatatgggg tgcgagctat ttggtcatca agttaccacc
     2161 aaaactggga tccttttttg agtttagtgc cagcaataca aaaaactagt aaaattctct
     2221 taggtccatt ggctataag
//
LOCUS       DASCTH010000098         1563 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000137541, whole genome shotgun sequence.
ACCESSION   DASCTH010000098 DASCTH010000000
VERSION     DASCTH010000098.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000137541"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="QGH35_02100"
     CDS             <1..810
                     /locus_tag="QGH35_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014081.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81357.1"
                     /translation="PGGGGDIDVPGYTGNVPAHIRQGVTQGVENFRNRAETAVDGGAD
                     VLKMIASGAVLAFGSLPGSPEMTPEEIAAVVAVGHKAGIKVTAHAHGAQSVKEAILAG
                     VDSIEHASLIDDEGIQLAKEHNVALSMDIYNGDYINIAGKEENWPEEFLRKNRETTAE
                     QRNGFTKAHRAGVRIVYGTDAGVYPHGDNAKQFVYMVRQGMTSIEAIQAATINAADVM
                     GWGNQVGQINPGFFADIIALRDNPISNIQALENIDVVIKGGQLYKLDGKTF"
     gene            884..>1563
                     /locus_tag="QGH35_02105"
     CDS             884..>1563
                     /locus_tag="QGH35_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HJL81358.1"
                     /translation="MAETEIQVPEVALTGIPFDISIADASNANLCNYSIEINKLAYQP
                     DDCMDSNSVNFSNIVIEEKGSYSISIVVDESEIATKEINVLWGWLSVLPPLFTILMAL
                     ISKSVIPSLFLGIWIGSFSILGLNITVLGQSFLDIIARYIKNSLANPDHAAIIIFTLM
                     IGGLVGIISKNGGMLGVVNSLTRFTTNAKRGQLITSFLGIAIFFDDYANTLVVGNTMR
                     KITDKLKIS"
BASE COUNT          470 a          291 c          359 g          443 t
ORIGIN      
        1 ccaggtggcg gtggtgatat cgatgttcct ggttatacag gcaacgttcc agctcacatt
       61 cgacaaggtg taacacaggg agtggaaaat tttaggaaca gagcggaaac agctgtcgat
      121 ggaggggcag atgtgttgaa gatgatcgca tctggtgctg tgttagcttt tggtagtctt
      181 cctggcagtc cagaaatgac tcctgaagag attgcagcgg tcgttgcagt gggtcacaaa
      241 gcaggtatta aggtgactgc ccacgctcat ggagctcagt ctgtcaaaga agccattttg
      301 gctggtgttg attcaattga acatgcatca ctcatagacg atgaaggcat ccaactggcg
      361 aaagaacata atgtcgcctt gtcgatggat atttacaacg gtgactacat taatatcgct
      421 ggtaaggagg agaactggcc tgaagaattt ttaagaaaaa acagagaaac aacagcagaa
      481 caaagaaatg gttttaccaa agcacatcgg gcaggagtaa gaatcgttta tggaaccgat
      541 gctggagtgt atccacacgg tgataatgcc aaacaatttg tttacatggt cagacaaggc
      601 atgacctcaa ttgaggccat ccaagcagcg accataaatg cagcggatgt gatgggttgg
      661 ggtaatcaag ttggccaaat aaatccaggg ttttttgcag atatcatcgc tctgcgagat
      721 aatccaatca gcaatattca agctttggag aatattgatg ttgtcattaa aggaggacag
      781 ctttataaac tagatggcaa aactttttaa tcacccaact atgaacatga tacataagaa
      841 attgctttcc atattattgc tattggtttg ctcattgggt gcaatggctg agactgaaat
      901 acaagtccct gaggttgcac tgacaggaat tccttttgat atttcaatag cagatgcctc
      961 aaatgccaat ctctgcaatt attcgataga aattaataag cttgcctatc aacccgatga
     1021 ttgcatggat tccaatagtg ttaacttcag caatattgtc atagaagaaa agggctcata
     1081 ctctattagt atcgtggtcg atgagtcaga gatagctaca aaggaaatca acgttttatg
     1141 gggatggtta tctgttttac ccccattatt taccattctg atggctctca tctccaagag
     1201 cgtgattcca tctttgtttt tagggatatg gattggatcg ttctctatcc ttggtcttaa
     1261 tatcaccgtg ttgggacaaa gttttctcga tattatcgca aggtacatca agaactcact
     1321 ggcaaaccca gatcatgcag cgataataat tttcactttg atgattggag gactggtcgg
     1381 aatcatatca aagaatgggg gcatgcttgg agttgtcaat tccctaacgc ggtttaccac
     1441 caatgcaaaa cgtggtcaat taataacttc ttttctgggc atagctatat tttttgatga
     1501 ttatgccaat accttagttg ttggcaatac catgagaaaa ataaccgaca aacttaagat
     1561 ttc
//
LOCUS       DASCTH010000099         2245 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000137887, whole genome shotgun sequence.
ACCESSION   DASCTH010000099 DASCTH010000000
VERSION     DASCTH010000099.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000137887"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="QGH35_02110"
     CDS             <1..505
                     /locus_tag="QGH35_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HJL81359.1"
                     /translation="QPGWFPLWVTDFPMFQMDDKEKKWSAEHHPFTAPKEMTSKELVS
                     NPGDAMSLGYDLILNGHEIGGGSIRIHSLEMQKAVFEVIGIDLQEAQEKFGFFLEALK
                     FGCPPHGGIAFGIDRLVMLMTDSDSIRDVIAFPKTQTANCLLTNAPSAPGKNQLKELG
                     LSTVSRK"
     gene            630..1490
                     /gene="cyoE"
                     /locus_tag="QGH35_02115"
     CDS             630..1490
                     /gene="cyoE"
                     /locus_tag="QGH35_02115"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HJL81360.1"
                     /translation="MRLIYLAAKVRLGFLIMACALAGIAVSPSSALSNVQIIVLALAV
                     LLSSSITGAFNQLYERDIDAVMLRTQHRPFVTGQFLPSNAWKLWLFFLSLIAIMIAYL
                     AANMEAALFLFAGSFTYAVIYTVWLKRKTWMNIVIGGFAGSFAVLVGSAATGSTFAPA
                     PLILAVILFLWTPPHFWALAIICKEDYSKASIPMLPLVASELVTVQAIFWHTLILVLL
                     SFSLMFYGMHWVYLLFAVTGGGYFVYTSYQLAVKKTPHWARKTFYASIIHLGLLLIGT
                     MIDAIIFVNV"
     gene            1513..>2245
                     /locus_tag="QGH35_02120"
     CDS             1513..>2245
                     /locus_tag="QGH35_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HJL81361.1"
                     /translation="MKTTIIFLVIVSFFSIAHGSRNVIQFVDKSLQSGVADPAINSSG
                     PTFVDYDNDGDIDIYVATEYHGINHGNRLFENQGSGTYIDVAQLRGVHNETGLGRGAA
                     WADYDNDGDIDMVISNMPPSGRSKHTPTTLYKNMLIETHQPNFKDVTREANLMRQGNV
                     DDQKIGGIGNTGAGVAWGDYDNDGDLDLFWKCAEYDVDNALFRNNGDGSFTDVSSEMG
                     IGILDLVVEANSQGSPNWTDVDQDGW"
BASE COUNT          637 a          399 c          487 g          722 t
ORIGIN      
        1 tcagcctgga tggtttcctt tatgggtaac cgattttcct atgtttcaaa tggatgataa
       61 ggaaaagaaa tggtcagctg aacaccatcc ctttacagca ccaaaagaga tgacatcaaa
      121 agagttagtc agcaaccctg gtgatgctat gtcccttggg tatgatttga tattaaatgg
      181 tcatgagatt ggaggaggat caattagaat tcattcactg gaaatgcaaa aagcagtctt
      241 tgaagtgatt ggcattgatt tacaagaggc acaagagaaa tttggatttt ttcttgaggc
      301 attaaaattt ggttgcccac cacacggcgg aatagcgttt ggtattgacc gattggtgat
      361 gttgatgact gattcagata gcattagaga cgttatagcc tttccaaaaa cccagacggc
      421 aaattgtttg ctaactaatg ctcccagcgc cccaggaaaa aatcagttaa aggaattggg
      481 tctctcaact gtttctagga aatagttgtc tttgtttaaa tttttttatc atttttattt
      541 tgattgcttt tagtcaataa taagcaccga tttcgaacaa aatcaccatt gaaaaaaaca
      601 ttgtgataac tactaatttt gtaaagaata tgaggctcat ctaccttgca gcaaaggtca
      661 gattgggttt tctgattatg gcttgtgctc tagctggtat tgccgtctca ccttcatcag
      721 cgttgtccaa tgtacagatt attgttcttg cactagcggt tttattatct tcctccataa
      781 ctggtgcttt taatcagctg tatgaaagag atattgatgc tgtaatgttg agaacccaac
      841 atagaccctt tgtcactggt caatttttac catccaatgc ttggaaactc tggctgtttt
      901 tcttaagtct aattgccata atgattgcct atctggcagc caatatggaa gctgcattat
      961 ttctttttgc cggctcattt acctatgcag tgatctatac ggtatggttg aaacgaaaga
     1021 cctggatgaa cattgttatc ggaggttttg caggtagttt tgcagttctt gttggatctg
     1081 cagctacagg atccactttt gcgccggcac cattaatcct agctgtgatt ttgttccttt
     1141 ggactccgcc ccacttttgg gcattggcaa ttatttgtaa agaagattac agcaaagcat
     1201 cgatacctat gctgccatta gttgcttccg agttggttac tgtacaggct attttttggc
     1261 acaccctgat tctagtttta ctgtctttca gcctgatgtt ttatggaatg cattgggttt
     1321 atttattgtt tgctgtaacc ggtgggggat attttgtcta caccagttat caattagcag
     1381 taaaaaagac accacattgg gctcgtaaaa cattttatgc atccatcatt catttgggct
     1441 tacttttaat tggtacaatg attgatgcta ttatatttgt gaatgtatag aagataatgc
     1501 ccttgcagat ttatgaaaac aacaattatt tttttagtga tcgtatcttt tttttcaatc
     1561 gcacatggat ctagaaatgt tattcaattt gtcgataaaa gcctacaaag tggagttgct
     1621 gatccagcca ttaatagctc aggcccaacc tttgtagatt acgataatga tggcgacatc
     1681 gatatttatg ttgccactga atatcatggg atcaatcacg gaaatcgatt atttgagaat
     1741 cagggatcag ggacatatat tgatgtagcc caattgagag gagttcacaa tgaaaccggg
     1801 ctaggacgag gcgctgcttg ggctgattat gataatgatg gcgatataga tatggtgatc
     1861 tccaatatgc ctccttccgg tagatcaaag cacaccccca ccaccttgta taaaaatatg
     1921 ttaatcgaga cccatcaacc caattttaag gatgtgactc gagaagcgaa tttaatgcga
     1981 caaggtaatg tcgatgatca aaaaattgga ggtataggca atacaggggc aggcgttgcc
     2041 tggggtgatt atgataatga tggcgatctt gatttgttct ggaaatgtgc cgaatatgat
     2101 gttgacaatg cattgttcag aaataatgga gatggaagtt tcacagatgt tagctcagag
     2161 atgggtatcg gcattttaga tctagtagta gaggcaaatt cacaaggatc acccaattgg
     2221 acggatgtcg accaagatgg ctggc
//
LOCUS       DASCTH010000100         1664 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000138362, whole genome shotgun sequence.
ACCESSION   DASCTH010000100 DASCTH010000000
VERSION     DASCTH010000100.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000138362"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(79..1059)
                     /locus_tag="QGH35_02125"
     CDS             complement(79..1059)
                     /locus_tag="QGH35_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HJL81362.1"
                     /translation="MKAVWYESIGEAEQVLNYGDLEDPQINDGEVLVKLNTSGVNPSD
                     VKIRAGARGELQFPRIIPHSDGGGIIVEVGEEVSEDRIGERVWIWNGAFGRAFGTCAE
                     LIVLPSSQAVAMPENVSFETAACLGIPASTAYYGIFADGPVKDQTILITGGAGAVGYL
                     GIQLAKWSGAHVISTVSGDDKATVAEAAGADLVVNYKTDNVIKAVNDFTKGHGVDRIL
                     EVEFGGNLSVSEHVIKNNGVIAAYGSVTEANPSVPFYNLMFKSVKLNTYLIYIVADSD
                     RANINQGINAALSDNALTPIIAKSFQLSETVNAHQSVEEGSIIGNVVINI"
     gene            complement(1056..>1664)
                     /locus_tag="QGH35_02130"
     CDS             complement(1056..>1664)
                     /locus_tag="QGH35_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81363.1"
                     /translation="VVDFFRDINSNLGGLDALINNAGISGPTANLEDVEFSDWENTLS
                     VNLNSAFLCSKEAIPMIRNSGGGSIINIGSSSSFFGTPLRSAYSASKWALIGLTKTWA
                     MELGQDNIRVNAICPGSVNGPRIEKVIKQEASERNTDPTMIKEAYLNQTSLRTFIDPE
                     EITGMVKYLLSPIASKISGQAMSIDGHTESLSQIRTKEDHNE"
BASE COUNT          455 a          373 c          316 g          520 t
ORIGIN      
        1 tcagatagtg ttgctttcat aggagtaaga aagagaatta gagtgatgag ggttcgacct
       61 atttgtttca ttattttttc aaatattgat cacaacattg ccaataatgg aaccttcttc
      121 cactgattga tgggcattaa cagtctcaga caactggaag gatttagcaa taatgggagt
      181 gagtgcatta tcactaaggg cagcattaat cccttgattg atattagccc tatcactgtc
      241 tgcaacgata tagataagat aggtatttaa tttcacactt ttgaacatca agttatagaa
      301 aggtacagag gggttagctt cagtaacaga gccataagcc gcaatcacac cattgttttt
      361 tatcacgtgc tcactgacag ataaatttcc accgaattca acctcaagta ttctgtcgac
      421 tccatgacct tttgtgaaat cattgaccgc cttaataaca ttatctgttt tgtagttcac
      481 cactagatct gcaccagcag cttcagctac ggtcgcttta tcatcaccac tgactgtaga
      541 gataacatgt gctcctgacc attttgcaag ttgtatgcct agataaccta cagctccggc
      601 acctccagtt attaatatag tttgatcttt tactggacca tcagcaaaga taccgtaata
      661 ggcagttgat gctggtatgc ctaagcaagc agcggtttca aatgaaacat tctcaggcat
      721 agcaaccgct tgcgaggagg gaagcacgat cagttcagca caggtcccaa aagctcttcc
      781 aaaagcaccg ttccagatcc atactcgttc accaatccta tcttcactga cctcttcacc
      841 aacttctaca ataataccgc caccatccga atgagggatg attcggggaa attgcaattc
      901 tcctcttgct ccagctctaa tctttacatc agaaggatta accccggatg tattcaattt
      961 gactaatact tcgccatcgt taatctgagg gtcttccaag tctccataat ttaggacttg
     1021 ttctgcttcc ccaatagact cataccaaac tgctttcact cgttatggtc ctctttggtt
     1081 ctaatttggc ttaagctttc tgtgtgtcca tctattgaca ttgcttgtcc tgaaatcttg
     1141 gatgcgatgg gtgacagtag atattttacc atcccagtaa tttcttctgg atcaatgaag
     1201 gttctcagtg aagtttggtt taaataggct tccttgatca tagtcggatc tgtatttctt
     1261 tcactggctt cttgtttgat taccttttca attctcgggc cattaaccga tccggggcaa
     1321 attgcattga cacggatgtt gtcttgaccc aattccatcg cccatgtttt tgtcaatcct
     1381 attaatgccc actttgatgc cgaataagca gatcgtaatg gggtgccaaa gaaggatgaa
     1441 cttgatccga tattgatgat cgaacctcca cctgagtttc gaatcatggg tatagcttct
     1501 ttgctacaca gaaatgcact gttgagattg actgataagg tattttccca atcagaaaac
     1561 tctacatctt cgagatttgc tgtgggtcct gatatgccag cattattgat caatgcatca
     1621 aggcctccga ggttactgtt gatgtctctg aaaaaatcaa ctac
//
LOCUS       DASCTH010000101         2773 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000142693, whole genome shotgun sequence.
ACCESSION   DASCTH010000101 DASCTH010000000
VERSION     DASCTH010000101.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000142693"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="QGH35_02135"
     CDS             complement(<1..1016)
                     /locus_tag="QGH35_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJL81364.1"
                     /translation="MKIKDIFLFSSYSVISQRMRSFLTSLGIAVGVVCVIFLTSLGQG
                     LQEYIVGQFTQFGTNIIGISPGKEQTMGMPLGVFGTVRPLSFEDAEALERLPIIETVV
                     PVSGGAGEIESADRMRRSMVVGTGSDYDIIVGSEDNMVGQYLPDDNPKTPRAYAVLGP
                     TMKEELFGTKNPLGLTVRVNSVRFRVVGVLPSKGDFLGMDLDDAIYIPIAKFNSMFNR
                     DGFQEIDLIHKDNVTADEAVASIRRLMISRHGGDDITITTQADMLESLGEIMKWLKFT
                     VAAFGGISLLVGGVGIFTIMTVAVNERTSEIGVLRAIGASRRRIQDVFLLESIFLAIL
                     GAILG"
     gene            complement(1018..2166)
                     /locus_tag="QGH35_02140"
     CDS             complement(1018..2166)
                     /locus_tag="QGH35_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011771932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJL81365.1"
                     /translation="MLLIATSIGVMAVILLTAVGEGARDYITGEFKDMGTNLLIVLPG
                     KNETTGGGSPVDLAGVAPRDLTVEDAISLRQIEAINRIAPIVVGETPAQFNGLERSVP
                     IIGTTPSFLAVRQWEMESGNFLPGVTLDRSPPVCVIGKEVESELFGKNNALGQWLRVG
                     DRRCRVTGIIGTEGRAIGIDVQKIVVMPVSLATALFNRPSLFRIIAEARNSWEMDHIK
                     KEIIRIIKKRHQGKEDITVIRQDAVMKSFDNIFNVLTMALGSIASISLFVAGILIMNV
                     MLVAVSQRQSEIGLLKAIGAKNKQINYLFITEAIILSGSGAILGLILGHTTTQVLKLA
                     FPTFNFLPPLWASFVSLIVALLSGIIFGLLPARRASKLDPVIALSGNK"
     gene            complement(2235..>2773)
                     /locus_tag="QGH35_02145"
     CDS             complement(2235..>2773)
                     /locus_tag="QGH35_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015280365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJL81366.1"
                     /translation="LLNILGLLDTPTSGNYVLSGVSTSKMNDDEMAHIRSTKIGFIFQ
                     SFHLIPRLTAAENVEMPMVLSGLNLKERKEKVDNALARVSLTDRSDHRPEQLSGGQRQ
                     RVAIARSIVMEPDVLLADEPTGNLDSTSSKEIIKLIEELNKGGLTLLVVTHDIEMGSR
                     SNRLIELLDGKIVKDKKN"
BASE COUNT          853 a          632 c          482 g          806 t
ORIGIN      
        1 cctaagatgg ctcctaagat agctagaaaa atagattcca ataagaacac atcttgaatt
       61 ctcctgcgtg aagcaccgat ggctcttaac actccaattt ctgaggttct ttcattgaca
      121 gcaactgtca tgattgtgaa tataccgact ccacctacta agagtgaaat tccaccaaaa
      181 gcagcaactg taaatttcaa ccatttcata atttcaccta aggattccaa catatccgct
      241 tgagtggtaa tggtaatgtc atcgccccca tgacggctaa tcattaatct tcttattgaa
      301 gccacagctt catctgctgt tacattatcc ttatgaatga gatcaatctc ttggaatcca
      361 tccctattga acattgaatt aaatttagct atgggtatat aaatagcatc gtcaagatcc
      421 atacctagaa aatcaccttt tgatggcaac acccccacca cacggaatct tacagagttc
      481 actctaactg ttaaccctaa tgggtttttt gtaccgaaca attcctcttt cattgtaggg
      541 ccaagaacgg cataagctct tggtgttttt gggttatcat caggcaagta ttgaccgacc
      601 atattatcct ctgagccaac aataatatcg taatcagagc cggtgcctac aaccattgat
      661 ctcctcatgc gatcagcgct ttcaatttca ccagcaccac cggaaactgg aactactgtt
      721 tcaataattg gtagtcgttc aagcgcttct gcgtcttcaa acgataacgg tctgacagta
      781 ccgaaaactc ccaaaggcat acccattgtt tgctctttgc caggtgatat gccaatgata
      841 ttagtaccaa actgagtaaa ttgtccaaca atatactctt gtaagccttg acccaaactc
      901 gttaagaaaa tcacacaaac aaccccaact gcaataccaa gagaggttag gaagctcctc
      961 attctctgag agattacaga gtaagaagaa aagagaaaaa tatctttaat tttcatatta
     1021 cttattccct gataaagcga tcacaggatc tagcttagaa gctcttctcg caggaagtaa
     1081 gccaaaaata atccctgata gcaaagccac aatgagagaa acaaatgatg cccatagtgg
     1141 cggtaaaaaa ttaaaagtag gaaaagccag ttttagaact tgtgtggtgg tgtgtcccaa
     1201 gatcagccca agaatagctc ctgatccaga tagaataata gcttccgtaa taaataaata
     1261 attaatctgc ttatttttag caccaatagc cttaagcaga ccaatctctg attgtcgttg
     1321 agagacagca actaacatca cattcataat aagaatccca gccacaaata agctaataga
     1381 ggcaatagaa cctaaagcca tggtcaagac attaaagata ttatcaaaag atttcatcac
     1441 agcatcttgc ctgatgaccg taatatcttc cttgccctga tgacgctttt ttatgattct
     1501 aatgatttct ttcttgatat gatccatctc ccaagaattt cttgcctctg ctatgattct
     1561 gaataacgag ggtcggttga acagtgcggt ggctaaactc acaggcataa ctacaatttt
     1621 ttgtacatcg atcccaatcg ctcttccttc agtgccgata ataccagtaa cacgacaacg
     1681 tctatcacct actcttaacc attgacctag agcattgttt ttaccaaaga gttcagattc
     1741 aacttctttg ccaatcacac acaccggagg ggatcgatcc agagtaacac caggtaaaaa
     1801 attaccgctc tccatttccc attgtctgac tgctaaaaaa gagggggttg tgcctatgat
     1861 tgggacagat ctctccaagc cattaaattg cgccggtgtt tctcctacga caattggtgc
     1921 aattctattg attgcttcaa tttgtcttaa actaatcgcg tcttcaacgg tcaaatctct
     1981 tggcgcaaca ccagctagat caacaggtga gcctcctcct gtggtctcat ttttccctgg
     2041 tagaacaata agcagattgg tgcccatatc cttgaattcc ccagtgatgt agtctctggc
     2101 cccttctcca acagctgtta aaagaatgac agccataacc ccaatggagg ttgcaatgag
     2161 taacattaat gttcgcaatg ggtatcgtat cagagtgcta aacgctaaaa ataatagatc
     2221 ataaaaacgc ataattaatt ctttttatct ttaacgattt ttccatcaag gagttcaatc
     2281 aatctattag agcgtgagcc catctcaata tcatgggtta cgaccagaag agttaaaccg
     2341 cccttattca gctcctctat tagtttgata atttctttac tggatgtgga atcaagattt
     2401 cctgttggtt catcagctaa taacacatca ggctccataa caattgatct ggctatggct
     2461 acacgttgtc tttgtccgcc tgaaagctgt tcaggcctgt gatctgatct gtcagtgagg
     2521 ctcaccctgg ccagagcatt gtcaaccttc tcctttcttt cttttaagtt tagacctgat
     2581 aagaccatag gcatttcaac attctccgcg gctgtcaacc ttggtattaa atggaaagat
     2641 tggaaaataa atccaatttt tgttgatcgg atatgagcca tctcatcatc attcattttt
     2701 gatgtactaa caccagacaa aacataatta cctgaggttg gtgtatccaa taatccaagt
     2761 atattaagta gtg
//
LOCUS       DASCTH010000102         2620 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000144302, whole genome shotgun sequence.
ACCESSION   DASCTH010000102 DASCTH010000000
VERSION     DASCTH010000102.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000144302"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="QGH35_02150"
     CDS             <1..873
                     /locus_tag="QGH35_02150"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HJL81367.1"
                     /translation="ASKHSSVSLTIINEKEMKRLKMGALLSVTAGSVEPAKLIVLKYS
                     GGISSQQPIAIVGKGITFDTGGISLKPSNKMDEMKYDMCGGAAAIGILQAVAELKLPI
                     NVISIVPACENLPSGNATKPGDIVTSMSGQTIEVLNTDAEGRLILADALTYSHRFKPK
                     LIIDMATLTGACIVALGHHLSGLMSNSDDLANKLLAAGEAAGDGTWRLPLGKKYMDQL
                     KSNFADIGNISGGGGGAGTVTAACFLQKFVKDIEWAHLDIAGVAWLEGNKKGATGRPV
                     ALMSEFLIQEANKS"
     gene            880..1323
                     /locus_tag="QGH35_02155"
     CDS             880..1323
                     /locus_tag="QGH35_02155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HJL81368.1"
                     /translation="MIKQKPDVLFHHFQSTSNQDYLLYVCKLVEKGFKENFKPIYIQT
                     KNHKQAEQLDKLLWTFRQESFIPHAILNDSETLTAPVQIGWHKNSLDAAEAIINLSEN
                     IPNANISTKKIHEIVDEDGILKEKARERWKHYKTEGFDLKVHHIK"
     gene            1339..>2620
                     /locus_tag="QGH35_02160"
     CDS             1339..>2620
                     /locus_tag="QGH35_02160"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HJL81369.1"
                     /translation="MKKVYNPQSIETKIYQSWEQGGYFSPSNKGEAYCIMIPPPNVTG
                     TLHMGHAFQDTIMDTLIRYYRMKGMNTLWQVGTDHAGIATQMVVERTLEKEGLSRLEI
                     GRENFTKKVWEWKQRSGDTISNQLRRMGASVDWQRERFTMDEGLSEAVTEVFVRLYEE
                     GLIYRGKRLVNWDPTLLTALSDLEVRQTEELGKLWHLKYPISGSADYLIVATTRPETM
                     LGDTAVAVHPKDNRYKKYIGKDIELPLCSRKIPIIADDHVDQEFGSGCVKVTPGHDFN
                     DFEIGKKHNLPIINIFTANAKLNDSVPKDLIDIERFKAREVIIEKLKSLDLLEKVDDY
                     KLTIPRGDRSGSILEPFLTNQWFVKTKPLAEPAIAAVKNGELKFVPENWSKTYFEWMN
                     NIQDWCISRQLWWGHRIPAWYDTNNNVYVGRSEKE"
BASE COUNT          910 a          453 c          546 g          711 t
ORIGIN      
        1 gcatccaaac attcatcggt gtctttaaca atcatcaatg aaaaagaaat gaaacggcta
       61 aaaatgggag ccttattgtc agtcaccgct ggatctgtgg agcctgccaa attaatagta
      121 ctcaaataca gtggggggat atcatctcaa caacccattg caatcgtcgg taaaggtatt
      181 acatttgata cgggtggaat ttcacttaaa ccatcaaaca agatggatga aatgaaatat
      241 gacatgtgcg gtggagctgc agcaatagga attcttcaag cagttgcaga acttaaactg
      301 ccgataaacg ttataagtat tgtcccggct tgtgaaaatt tacccagtgg caacgcaaca
      361 aaaccagggg atattgttac ctcaatgtca ggacaaacta ttgaagtact caatactgat
      421 gctgaaggca ggcttattct cgctgatgca ttgacttatt ctcatcgctt caaaccaaaa
      481 ctgattattg atatggcgac tttaactgga gcttgtatag ttgcacttgg tcatcatctc
      541 tctggcttga tgagtaattc agatgattta gctaataagt tactggctgc aggagaagct
      601 gcaggagatg ggacatggag attaccatta ggcaaaaagt acatggatca attaaaaagt
      661 aattttgccg atattggtaa tattagtggc ggtggtggtg gagcaggtac agttactgcg
      721 gcttgttttc tccaaaaatt cgtgaaagat atcgaatggg cacatcttga tattgcagga
      781 gttgcttggc ttgaaggtaa taagaaggga gccacaggca gaccagttgc cttaatgtct
      841 gaattcctga ttcaggaagc aaataaatcc tagcttagga tgattaaaca aaaaccagat
      901 gttttatttc atcatttcca atccactagc aatcaagatt atttacttta tgtatgcaaa
      961 ttagttgaaa aaggattcaa agaaaatttt aaacccattt acatacaaac aaagaatcac
     1021 aaacaagcag aacaattgga taaattactt tggacttttc gacaggaaag ttttatacct
     1081 catgcaattc taaatgattc tgaaaccttg actgctccgg tgcaaatagg ctggcataaa
     1141 aattcattag atgctgctga agccatcatc aacttgagtg aaaacattcc caatgccaat
     1201 atttcaacaa aaaaaataca cgagattgtt gatgaagatg gaatcttaaa agagaaagct
     1261 agagaacgat ggaaacacta taaaaccgaa ggatttgatt taaaagtgca tcacattaag
     1321 tgatcaagat aataagcagt gaaaaaagta tacaatcccc aatccattga gaccaagatt
     1381 tatcaatctt gggaacaagg aggttatttc tctccgagta ataaaggaga agcatattgc
     1441 atcatgattc ccccaccaaa tgttactgga acgcttcaca tgggacatgc ttttcaagac
     1501 actattatgg atacgttaat aagatattat cgaatgaaag gcatgaacac tctttggcaa
     1561 gttggcacag atcatgctgg aattgcaacc caaatggtcg tggaaagaac acttgaaaaa
     1621 gaaggtttat ctcgtttgga aataggtcgt gaaaatttta ctaaaaaagt ttgggaatgg
     1681 aaacaacgct cgggagatac aatctcgaat caattgagaa gaatgggtgc atcggttgat
     1741 tggcaaagag aacgctttac tatggatgaa ggtctttctg aagccgtgac tgaagtcttt
     1801 gtgagactat atgaagaagg attgatctat cgaggtaaac gattggttaa ttgggatcca
     1861 acactgctta cagctttatc agatcttgaa gtaagacaaa ccgaagaact tgggaagcta
     1921 tggcatctta aatacccaat ttcgggctca gctgactatc ttatagttgc aaccacgaga
     1981 cccgaaacca tgcttggtga tacagcagtg gctgtccatc caaaagacaa tcgctacaaa
     2041 aaatacattg gcaaagacat agaactacca ctttgttcaa ggaagatacc gataatcgct
     2101 gatgatcatg tcgatcaaga atttggaagc ggctgtgtta aagtcacacc aggacatgac
     2161 tttaatgact ttgaaattgg caaaaaacac aacttgccta tcatcaacat tttcacagca
     2221 aacgcaaaat taaacgacag cgtaccaaaa gatttaattg atatagagcg ttttaaagca
     2281 cgtgaagtca ttattgaaaa attaaaatcg cttgatttat tggaaaaagt tgatgattac
     2341 aaactgacca ttcctagggg agatagaagc ggttctatac tagaaccgtt tttaactaat
     2401 caatggtttg taaaaactaa acctttggct gagcctgcaa ttgctgctgt aaaaaatgga
     2461 gaattaaaat tcgttcccga gaattggtca aaaacatatt tcgagtggat gaataatatt
     2521 caagattggt gtatcagtcg tcaattgtgg tgggggcatc gtattccagc ttggtatgac
     2581 actaataaca atgtttatgt tggcagatcc gaaaaagaag
//
LOCUS       DASCTH010000103         3865 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000146648, whole genome shotgun sequence.
ACCESSION   DASCTH010000103 DASCTH010000000
VERSION     DASCTH010000103.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000146648"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="QGH35_02165"
     CDS             <1..462
                     /locus_tag="QGH35_02165"
                     /inference="COORDINATES: protein motif:HMM:NF039476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HJL81370.1"
                     /translation="ESTWEHLKMYFWPGLIYALIQYTYTRDIANNYWLAKLISLIATP
                     LGIIISFYGYLSIALPVYGTGFFVMDLMTMVVGLSCGSFCAYKLMMKSSIHSVPKNYV
                     LSGYLILTSMFSILTYFPPKLFLFENYFGYEYKGEYGILNNYEPYRVFRKN"
     gene            complement(464..1273)
                     /locus_tag="QGH35_02170"
     CDS             complement(464..1273)
                     /locus_tag="QGH35_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HJL81371.1"
                     /translation="MDRKDKEKQILIELLALSAVEHLSFEDEQKLAQLLLHYPEYTKD
                     DFAKTAALSQIGFYLMDDTANETLPSEIRNKILKTYDSTSDEKNTLPGFNFNSLFKTL
                     IKPAYAWSITVMLTIGLSYSMITFKTYENNYRYLPLKRMVLKNTAKDLMNHPWYGKTS
                     DFETIQGHVIWSHDKQKGFITITGMPINDSSLNQYQIWIVDPIKYLNPVDGGVFDITT
                     SEKEVIIPINPKLPIFNARAFAITLEQPGGVVVSSQPLLLTAPKERPKQKI"
     gene            complement(1266..1898)
                     /locus_tag="QGH35_02175"
     CDS             complement(1266..1898)
                     /locus_tag="QGH35_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019265.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HJL81372.1"
                     /translation="MELIINNLSIKDTAESGMANTNPKANENLLAKVASGNPEAFEEL
                     INKYGNLIWSIARRYLSNQNEAEDAVQEIFVALWQSAGRFDPNKASEITFISMITRRR
                     LIDNLRKNNKHKVLKSIEDSLSDDVFSQKSNLEENAEISLVIRALKKLDIQDQELLSL
                     SIYQGYSHLEIAKLLNLPLGTVKTKIRRNLMKIRDDIKRINTKVKHLYHG"
     gene            complement(2016..2690)
                     /locus_tag="QGH35_02180"
     CDS             complement(2016..2690)
                     /locus_tag="QGH35_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HJL81373.1"
                     /translation="MEKTILIVEDELKLANILVEYLQKEGFKTKHIDIGTRVIPWIKK
                     HNPDLILLDLMLPAMNGKEICQEIRTFSQLPIIMITAMVDEIDRLIGLEMGADDYICK
                     PFSPKEVVARVKAVLRRVDPEYIKNNLSNGFEIDRERFSITLDGIKLDLTPVEFRLLA
                     MFVEHSGRVYNRDQILNMIFDDGRIVLDRTVDTHIKNLRHKLKVINPKTDYIRSLYGV
                     GYSFEN"
     gene            complement(2694..>3865)
                     /locus_tag="QGH35_02185"
     CDS             complement(2694..>3865)
                     /locus_tag="QGH35_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJL81374.1"
                     /translation="IMTSGNTSRGREFTRYRFTFGQPKKNKNLLRGRLSLYDAKDQLL
                     IGFKTKEVNQRAIRTVPIYFESNQVGTLALLAPPGLIERIDMQFAEAQQSTLYGAAFI
                     ALIFAGLISLLFTMNFTGPIRRLALATKELIGGEFKTRVKIKNKDEMGRLSRDFNILA
                     KTLDKNSESQKQWIADISHELRTPLAILKSEIESIHDGIRNFDAESLISLSNEVERLN
                     SLVEDLYELALSDIGAMKYKMADTNLSDLLSQTLTGYTDRCVQNELQLNTSIDDSFEI
                     IGDEKRLGQLFSNLLENSLRYTDAPGTIEVAIFEESSNVVVTFSDSAPSVDQESMDKI
                     FERLFRKDSSRSRENGGAGIGLSICKAIVEAHSGTINAQSSALGGLFIEIKFNKK"
BASE COUNT         1124 a          698 c          673 g         1370 t
ORIGIN      
        1 gaaagtacat gggagcatct aaaaatgtat ttttggcctg gattaatcta tgcactcatc
       61 cagtatactt atacgagaga cattgctaat aattactggc tagcaaaatt aatttcactg
      121 atagcaacac cacttggaat tattatctct ttttatgggt atctttccat agcccttcca
      181 gtttatggaa caggtttttt tgtaatggat ttaatgacaa tggttgttgg tctttcttgt
      241 ggttctttct gtgcctataa gttaatgatg aaatcatcca ttcattcagt tccaaaaaat
      301 tatgttcttt ctggatactt gattttgact tcgatgttct caatattgac atattttccg
      361 cctaaattat ttctttttga gaattatttt ggttatgaat acaaaggtga gtacggaatc
      421 ctgaataact acgagcctta tcgcgttttt agaaaaaact agattaaatt ttctgcttag
      481 ggcgttcttt tggagcagtc aatagcagtg gctgggatga aacaacaaca ccaccaggtt
      541 gttctagcgt aattgcaaaa gctctggcat tgaagattgg taacttcgga ttgataggaa
      601 tgatcacttc tttctcggat gtggttatat caaaaacacc cccgtcaacg ggattaagat
      661 acttgatggg atcaacaatc caaatttggt attgatttag ggatgaatca ttgattggca
      721 ttcccgttat tgtaataaat cctttttgtt tatcatgact ccagatgaca tgaccttgaa
      781 tggtttcaaa gtctgatgtt tttccatacc aaggatgatt cattaagtct tttgcagtgt
      841 ttttcagaac cattcttttt aagggcaagt agcgataatt attttcatat gttttaaagg
      901 taatcattga ataggacaaa ccaatcgtta acatcaccgt tatactccaa gcatatgctg
      961 gttttattag agttttgaat aagctattaa aattaaagcc tggaagagta tttttctcat
     1021 ccgatgttga atcataagtc tttaggattt tatttctgat ttcactcggt aaagtttcat
     1081 tggcggtatc atccatcagg tagaaaccta tttgtgacaa tgcagcggtt ttagcaaagt
     1141 catcctttgt atattctgga tagtgtaata ataattgagc caatttttgc tcatcttcaa
     1201 atgataaatg ctctactgct gacagtgcta atagttctat caatatttgt ttttctttgt
     1261 cttttctatc catgataaag atgtttaact ttcgtattta tgcgtttgat gtcgtctcta
     1321 attttcatta aattcctgcg aatcttagtc ttgacagtac ccagtggtag attaagtaat
     1381 ttagctattt ctaagtgcga gtatccttgg taaattgaaa gagagagtaa ttcttggtct
     1441 tgtatgtcga gttttttcaa agctctaata actagagaga tttcggcatt ttcttctaga
     1501 tttgattttt ggctaaaaac atcatcactt aaagaatctt caatcgattt taaaactttg
     1561 tgtttattat tcttccttaa attatcaatt aatctacgcc ttgttatcat tgaaataaaa
     1621 gtaatttctg atgctttgtt tgggtcaaaa cgaccagcag attgccataa tgcaacaaat
     1681 atttcttgaa cagcatcttc agcttcattt tgattggata agtatcgtct ggcaatagac
     1741 caaattaagt tgccatactt attaattaac tcttcaaatg cttcaggatt accactagcc
     1801 actttggcta acaggttttc gttggctttt ggattagtgt ttgccattcc actctcagca
     1861 gtatctttga tagacaggtt gtttataatc agttccaaca aaggcaccac ttagttcttt
     1921 gatagtaatt agacatctaa ttaacatatt tcggtcatcc attagaacta gattcaagtt
     1981 tatgctcaca aatagtcaaa atagagcaaa tttaatcagt tttcaaaact ataaccaaca
     2041 ccatacaatg accggatgta atcagtcttg gggttaatta ctttgagctt gtgtcgtaga
     2101 ttcttaatat gagtgtctac tgttcgatcc agaacgatac gcccatcgtc aaaaatcata
     2161 tttaggattt gatccctatt gtaaactcgt cctgagtgct caacaaacat ggcaagtaat
     2221 ctaaattcaa ctggagttag atctagcttt attccatcaa gcgtgattga gaatcgttct
     2281 ctatcgattt caaacccatt cgataggtta tttttgatat attctggatc gactcgtctt
     2341 aatacagcct tgactcttgc cactacttcc ttggggctaa atggtttgca gatataatca
     2401 tctgcaccca tctcaagtcc gatgagacgg tcaatttcat caaccatagc tgtgatcata
     2461 ataataggta attgggagaa tgttctaatt tcctgacaaa tctctttacc attcatagcg
     2521 ggcagcatta aatccaataa aattaaatcg ggattatgtt tcttaatcca tggaatgact
     2581 cgtgtgccga tatcaatgtg ttttgtttta aagccttctt tttgtaaata ctcaaccagt
     2641 atatttgcca gcttcaattc atcttcaaca attaagatcg ttttttccat cacttatttc
     2701 ttgttgaatt taatttcaat gaatagacca cccaaagcag aggattgagc attgattgtg
     2761 ccagaatggg cttccacaat tgctttgcaa atagataagc ctataccagc gccaccgttt
     2821 tccctagaac gggatgaatc ttttctgaag agccgttcaa aaatcttatc catagattct
     2881 tgatctacac ttggagcaga atctgaaaag gtaacgacaa cattgctgct ttcttcaaag
     2941 attgcaacct caatagttcc tggtgcatcg gtataacgta gagaattttc tagtaagttt
     3001 gaaaacaact gacccaatct tttttcatct ccaatgattt caaatgaatc atcaattgaa
     3061 gtgtttaact gaagttcatt ttgcacacat ctatctgtat atcctgtgag ggtttgtgat
     3121 aaaagatcag aaaggtttgt atcagccatc ttatatttca tggcaccaat gtcagagagc
     3181 gccaattcat agagatcttc tactagagaa tttaggcgtt ccacttcatt ggataacgag
     3241 atcagtgatt cagcatcaaa attcctaatt ccatcatgaa tggactctat ttcacttttt
     3301 aatattgcca agggagttct aagttcatga gaaatatcag caatccattg cttttgactt
     3361 tctgagttct tatccaatgt ttttgcaaga atattaaaat ctctggataa tcttcccatt
     3421 tcatctttat ttttgatttt gactcttgtt ttaaattccc caccaatgag ttcttttgtt
     3481 gctaaggcta gccttctgat aggaccagta aaattcatcg tgaaaagcag cgaaatcagt
     3541 cctgcaaaaa ttaatgcaat gaaggcagct ccatatagag ttgattgttg tgcttctgca
     3601 aattgcatgt caatgcgttc aattagcccg ggaggtgcta gcagagcaag tgtcccaact
     3661 tggttacttt caaagtaaat agggaccgtt cttatcgcac gctgatttac ttctttggtc
     3721 ttgaaaccga taagcagttg atccttggca tcgtagagac taagccttcc tctgagaaga
     3781 tttttatttt ttttaggctg tccaaaggtg aaacggtatc tagtaaactc tcttcctctg
     3841 gaagtgttgc ctgatgtcat gatta
//
LOCUS       DASCTH010000104         2915 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000146957, whole genome shotgun sequence.
ACCESSION   DASCTH010000104 DASCTH010000000
VERSION     DASCTH010000104.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000146957"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="QGH35_02190"
     CDS             <1..1232
                     /locus_tag="QGH35_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81375.1"
                     /translation="QADLKRQQIDFITKFDIGEMAGGPVGMVVGAEYSHLSYAVLNDP
                     ASNALIIAGTAGGDDIAAERSRKSAFFELGMPVLDNLNISVAGRYDDYSSPGIGSNFS
                     PQLTIAYKPIDWILLRGTYGEGFRAPAMDDLYGNQSESFPSGIDIVGCAAGVSVCTAT
                     QYRALYGGNPELMPEDSEHYTLGFVISPMPELDLAFGFWHTEYANLITTSSINREFAA
                     EAAGLTNYVYRYGAGEEGPAGTVKYISLQYNNFAGVEAEGLDFEINYNIDTENAGTFE
                     IGLIAAKYTKYIVQTYPESPKDEFQGEMGLPDLRINPHVYWGKGDWNAALTGYYLSGQ
                     DEDYGGANYSVGSHMEMSLQIGYQLPWDASFTVGVINIGNVEPEQNSDWYGWEPFDFT
                     LYSTWGRTVYLRYDQDL"
     gene            complement(1284..2039)
                     /locus_tag="QGH35_02195"
     CDS             complement(1284..2039)
                     /locus_tag="QGH35_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJL81376.1"
                     /translation="MESTECRTLIEKNQCMEAIRVCKIEAEQGDAKAQTLLGFLYSGT
                     SNGDFRKNYKESFRWISLAAEQDFTKAQLAMAEMYMRGEVVPENAKKAKIWYEKAAEK
                     GNLDGINGLARLYRYGTGVRKDYEKAFELYQQAALQNHTRSQVGVGLSYRDAKGVKKN
                     VIKAYAWLSLAAGRMDDRALKDLQKRWENEREEMDKTIPQCKYVLRTEKMAEIFAVSY
                     AKRALFDLKQRMGLKQIKKAKDLMVQLRAEISP"
     gene            2199..>2915
                     /locus_tag="QGH35_02200"
     CDS             2199..>2915
                     /locus_tag="QGH35_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81377.1"
                     /translation="MRFLILVLIVFAHSNSFAVKQFPVEHFFQDPQMLNPQLSPDGDY
                     LAALLPFNINEETQKVCKQRTKKLIETFKKQGYGDVSNNPELLKQLKQRFIQPDKGVH
                     LCDRSRRNIVVIWLSDPNPDCQQGNFGRCERVRVTQLRSQNVSGFFWANNERIIFETG
                     GDQLNGISGYADTLGLYAVNKDGSKDKQLVKPADADGNVVPLNLLKDDPDHILVMRND
                     RKRYLYDIYKMNVFTGNFFRE"
BASE COUNT          843 a          573 c          583 g          916 t
ORIGIN      
        1 atcaagctga cttaaaacga cagcaaattg acttcattac caaatttgat attggtgaaa
       61 tggctggtgg tcctgttggt atggttgttg gtgcagaata ttcacactta tcatacgcag
      121 tactgaatga tccagcatcc aatgcattga tcattgcagg aacagctgga ggtgatgata
      181 tcgctgcaga aagaagcaga aaatctgcat tcttcgaact tggtatgcct gtcctagata
      241 atttaaatat cagcgttgct ggtcgttatg acgactatag ttctccgggt attggtagca
      301 acttctcacc acaattaacc atagcttaca aacctattga ctggattttg ttgagaggca
      361 cctatggtga agggttccgt gctccagcaa tggatgactt atatggtaat cagtctgaaa
      421 gtttcccaag tggtattgat attgttggct gcgctgccgg cgtctcagtt tgtactgcca
      481 cgcagtatcg tgccctctat ggaggtaacc cagaactgat gccagaggat tctgaacact
      541 acacacttgg ttttgttatt agtccaatgc ctgaattaga cttagcattt ggcttctggc
      601 acaccgagta cgcaaacttg attactacat ctagtattaa tcgtgagttt gcagctgaag
      661 cagctggttt gacaaactac gtttatcgtt atggagccgg tgaagaaggt ccagcaggaa
      721 ctgttaaata catttctttg caatacaata actttgctgg tgttgaagct gaaggtcttg
      781 actttgaaat caattataat attgatactg aaaatgctgg cacatttgaa attggactga
      841 tagcggcaaa atacaccaag tatattgtcc aaacctatcc tgaaagccct aaagatgagt
      901 ttcagggaga gatgggatta ccggatcttc gaattaaccc tcatgtttat tggggtaaag
      961 gtgactggaa tgccgcatta acgggttatt atctttcagg tcaggatgag gactacggtg
     1021 gtgctaatta ttctgtaggc agccacatgg aaatgagtct tcaaatcgga tatcaactcc
     1081 catgggatgc atcattcaca gtcggtgtta ttaacattgg caatgtggaa cctgaacaga
     1141 actctgattg gtatggctgg gaaccatttg atttcaccct gtatagcacc tggggtagaa
     1201 cggtttacct tcgatacgat caagatctct aatttattag agtagttcga tcataagagc
     1261 gggcatattg cccgctcttt tttttatggg ctaatttctg cacgtagctg gaccattaaa
     1321 tctttggctt ttttgatttg cttgagaccc attctctgtt tcaaatcgaa caaagctctt
     1381 ttggcataac tgaccgcaaa tatttcagcc attttctcgg ttcgtaaaac atatttacac
     1441 tgaggaattg ttttatccat ctcttctctc tcattttccc agcgtttttg tagatctttc
     1501 agcgcccggt catccatcct acccgcagct aatgacagcc aagcataggc tttgatgaca
     1561 tttttcttca ctccttttgc atccctatag gataatccga ctccaacttg agatcttgtg
     1621 tgattctgca gtgctgcttg ctgataaagc tcaaaagctt tttcatagtc ttttctaact
     1681 ccagtgccat aacgatacaa tcttgctaaa ccattgatac catctaagtt tcctttttca
     1741 gctgcttttt cataccagat tttagccttt ttggcattct caggaacaac ttctcctctc
     1801 atatacattt ctgccatagc tagttgagct ttagtgaaat cttgttcagc agcgagacta
     1861 atccagcgaa acgactcctt ataatttttt cgaaaatcac catttgatgt tccagaatat
     1921 aaaaatccaa ggagtgtttg ggccttggca tcaccttgtt ccgcttcaat cttgcaaact
     1981 ctgatggcct ccatgcactg attcttctcg atcaaggttc tgcattcggt tgactccatt
     2041 tctgcaaatg ctggtgataa taaaaaaact aatattaaaa atattatctt agataaatgt
     2101 agcatgggta aatgttaact aattttcttt gaagcaacta gatctttaag aaaattttag
     2161 atatggtttt acatcttaca aacacatagg ggttttaaat gaggttttta attttagtgt
     2221 tgattgtttt tgctcactca aattcatttg ctgttaagca atttccagtg gagcattttt
     2281 tccaagaccc acagatgctc aatcctcagt tatctccaga tggggactat ctagcagcac
     2341 tgctaccttt taacatcaac gaagaaactc aaaaagtctg caaacagaga acaaaaaaat
     2401 taatagagac attcaaaaaa caaggctatg gagatgtatc taataatcca gaattactga
     2461 aacaattaaa acaaagattt atacaacccg ataaaggggt ccatctctgt gatcgcagca
     2521 gacgcaacat tgtagtgatt tggctgagtg atccaaaccc ggattgtcaa caaggcaact
     2581 ttggacgatg cgaacgggtg cgtgtgactc agctcagaag tcaaaacgtt tctggttttt
     2641 tttgggccaa caatgaaaga attatttttg aaactggtgg agatcaactt aatggaatct
     2701 caggttatgc agacactctt ggtctttatg cagttaacaa agatggtagc aaagacaagc
     2761 aactggtgaa accagcagat gctgatggga atgttgttcc attaaatctt ctcaaagatg
     2821 atccagatca catcttagta atgagaaatg atcggaaaag atacctttat gacatttata
     2881 aaatgaatgt ttttaccgga aatttttttc gagag
//
LOCUS       DASCTH010000105         2786 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000152700, whole genome shotgun sequence.
ACCESSION   DASCTH010000105 DASCTH010000000
VERSION     DASCTH010000105.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000152700"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="QGH35_02205"
     CDS             complement(<1..516)
                     /locus_tag="QGH35_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81378.1"
                     /translation="MNLITTQEYKTYKKIEHNKDDNQLNVLVSAVSALVKTYTGNSII
                     DYAVANKVENFDIHDNYTSELFLTESPLTSVGLLQERDSIANSYTTLTENTDYYVDVE
                     HDRVYRINGDAGIKYFPKGFAAVKVTYRAGYEICPEDLKLAIYDLITYYLKEEYKGRR
                     SMAGATLQNEAS"
     gene            complement(567..2579)
                     /locus_tag="QGH35_02210"
     CDS             complement(567..2579)
                     /locus_tag="QGH35_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016471.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HJL81379.1"
                     /translation="MKKIFNLTSNFKAVSENEDGSVSIKGYASTKDQDRAGDIINPDA
                     WSKGGIGDYENNPIILFNHDYHNPIGKTTELGVDDIGLKIKGKISQSAGKIRDLIKEG
                     VLGAFSVGFRVKDADYIEETDGYRIKDAELFEISVVSVPANQAATFSVAKAFDSDDEY
                     QDWKSNFVKPGQSINIDSPEATVKQTVLKETKMSDTKENFDLEEFAKEVAQKTAATIA
                     MQHAEQKAKAEAEAQEAAENEAAAKVEIQEKKAQVESIVKAGTEGAERLVSDLEGRVD
                     ENYSNLEQVVEELKDELKTKSEEIMNIRESKRTFSNKGEKKGFYNEQDINDAWLLGKA
                     LGKPIEDTDYGKSVMEKQNAHSGVGVSSADFEQEVSTNVERDIQNQLIIAPMFSEIAM
                     NAATMILPVLPDAGYAEFQSNQTASGSSPHGNLAERGDTYGSAYGGIDLTERSLTTKK
                     LISQSYLGNETEEDAIMPVLPLIRESMIRSHARSIESGLLVGNLADGPFGTSGACFDG
                     LISLASADSDKTQSTTAFASESLTAANLLAARKNMGKYGVNAADVVYIVNQTEWFNLM
                     DDAEFQDVNLVGESLASKVKGSVGTVYGSPVVLCDEFATPAVGKYYAVAVNPKMFIKG
                     RLRGMTIESDYEVANQRRVLVASQRIGFIDIIDAATSKWALQYKAS"
     gene            complement(2580..>2786)
                     /locus_tag="QGH35_02215"
     CDS             complement(2580..>2786)
                     /locus_tag="QGH35_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81380.1"
                     /translation="AQDDAPHRARAIRKLTGSWARMIQIIKVNFPEEYEKATKPAPAP
                     KPSAKPKFKAKPKIKANSAGKKGK"
BASE COUNT          746 a          581 c          454 g         1005 t
ORIGIN      
        1 cgacgcttca ttctgaagtg tagcacctgc catcgatctt ctacccttat attcttcttt
       61 aagataataa gtaataagat catagattgc cagttttaaa tcttcaggac aaatttcgta
      121 acctgcgcga tacgtgactt tgacagctgc aaagcctttt ggaaaatatt taatacctgc
      181 atcaccattt attctataaa ctcgatcatg ctccacatcc acataatagt cagtattttc
      241 agttaaagtc gtatagctat tagctattga atctctttcc tgtaatagtc caacactagt
      301 aagtggtgat tctgttaaaa ataattctga ggtataatta tcatgaatat caaagttctc
      361 tactttatta gctacagcat agtctattat tgaatttcca gtatatgttt tgactaatgc
      421 acttacagca gaaactaaaa catttaattg attatcatct ttattatgct ctatcttttt
      481 gtaagttttg tactcttgag tagtaattaa attcatttat ttttcctaaa aatctgggag
      541 gcaataacct cccagattac ccagctttaa gatgctttgt attgtagtgc ccacttagaa
      601 gtagctgcat cgattatatc gataaagcca attctttgag acgcaacaag tactcggcgt
      661 tggttagcaa cttcatagtc gctttcaatt gtcattcctc ttagacgtcc tttaatgaac
      721 atcttagggt tgactgctac agcgtagtat ttacctacag caggggttgc gaattcatcg
      781 catagaacaa ctggagaacc ataaaccgtt ccaactgatc ctttaacttt agaagctaaa
      841 ctttcgccaa ctaagttgac atcttgaaat tcagcgtcgt ccattaaatt gaaccactca
      901 gtttggttga caatataaac aacatctgct gcgttaacac catatttgcc catattctta
      961 cgtgctgcta atagattagc tgctgtaagg gattcacttg caaatgcagt tgttgattga
     1021 gtcttatcac tatccgctga tgcgagagaa ataagtccat caaaacatgc accacttgtt
     1081 ccaaatggtc cgtctgcgag gtttccaact aaaagtccgc tttcgataga tctagcgtgt
     1141 gaacgaatca ttgattcacg aattaatgga agaacaggca taattgcatc ttcttcagtt
     1201 tcgttaccaa gatatgattg tgaaatcaat ttcttagttg tcaatgatct ttctgttaaa
     1261 tctataccac cataggctga tccataagta tcgcctcttt ctgccaagtt tccatgtggg
     1321 ctagagcccg atgctgtttg gttactttga aattctgcgt aacctgcatc tggaaggaca
     1381 ggaaggatca tggtagctgc attcattgca atttcgctaa acataggagc gataatcaat
     1441 tgattttgaa tatctcgttc tacgtttgtt gaaacttctt gctcgaaatc ggcagatgaa
     1501 acacctacac ctgagtgtgc attctgtttt tccataacgg atttaccgta atcggtatct
     1561 tcgataggtt tacctaatgc tttacccaag agccatgcat cattaatatc ttgttcatta
     1621 taaaagccct ttttctctcc tttattggag aaagttcttt tagattcacg aatattcatg
     1681 atttcttctg acttggtttt caattcatct tttagctctt ctacaacttg ttctaggttt
     1741 gaatagtttt cgtcaacacg tccttctaga tcagatacta atcgttctgc tccttcagtt
     1801 cctgctttaa caatactttc tacctgtgct ttcttttctt gaatttctac tttagcagcg
     1861 gcttcgtttt ctgcggcttc ttgagcttca gcttccgctt tagctttttg ctctgcatgt
     1921 tgcatagcaa tagttgcagc cgttttttgt gccacttctt tagcaaactc ttcaagatcg
     1981 aagttttctt ttgtatcaga cattttcgtt tccttgagta cagtctgttt gactgttgct
     2041 tctggtgagt ctatattaat agattgacca ggtttgacaa agttagattt ccaatcctga
     2101 tattcatcat cagaatcgaa agcctttgcc acagagaagg tcgctgcttg attagcgggt
     2161 actgatacca cactaatttc aaacaattct gcgtccttga ttctatagcc gtcggtttcc
     2221 tctatataat cagcatcctt gactcggaaa ccaacgctaa aagctccaag aacaccttct
     2281 ttaattaaat ctctaatttt tccagctgat tgagatattt tcccttttat ctttaatcca
     2341 atatcatcaa cacctaactc agttgtttta ccgataggat tatgataatc atggttaaaa
     2401 aggattatag gattattttc ataatcccct attccacctt tcgaccaagc atcaggattt
     2461 ataatatctc cagctctatc ttgatcttta gtgctagcgt aaccttttat acttacgcta
     2521 ccatcttcgt tttcgcttac tgctttaaaa ttagatgtta gattaaaaat ttttttcatt
     2581 tatttcccct tttttcctgc tgaattagcc ttaatttttg gttttgcctt aaattttggc
     2641 ttagcagaag gcttaggtgc tggagcaggc ttagtagcct tctcgtattc ctctggaaaa
     2701 ttcaccttaa ttatttgtat cattctagcc catgaacctg ttaactttct aattgctctt
     2761 gctctatgtg gagcatcatc ttgtgc
//
LOCUS       DASCTH010000106         2331 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000155566, whole genome shotgun sequence.
ACCESSION   DASCTH010000106 DASCTH010000000
VERSION     DASCTH010000106.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000155566"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="QGH35_02220"
     CDS             complement(<1..578)
                     /locus_tag="QGH35_02220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81381.1"
                     /translation="MKTVVITGSTRGIGLGLAREFLKRGHQVVVSGTSKRSVERALGQ
                     LDGLGEMIGQPCQVQDYESVQSLWNKGFEHFGKIDIWINNAGVSTTKTILDRLQLDEI
                     HNTVDTNLIGSIFCTKVAAKEMLKQGFGQIYMFEGFGSNGQLQPGITVYGSTKRALRY
                     FTAAVANEYQDTPLIIGSLSPGIVATDLLLFSS"
     gene            complement(618..1784)
                     /locus_tag="QGH35_02225"
     CDS             complement(618..1784)
                     /locus_tag="QGH35_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81382.1"
                     /translation="MAIIFLVTFIGMMGFGIILPLFPFYAERLGAGPEVITLTMAAFS
                     LGQFIAAPIWGQLSDSIGRKQTLVITLFGSGISYLILAYAPNLEVVILSRILSGLMAG
                     NIAIAFAYVSDITTDENRSAGLGKVSAGLGLGFMTGPAIGGLLAGNNVENANYLLTAL
                     SAAGINFIAMFAALFFLKESLDDANRKPFEGLSKIIELDTFQPSKIILPLVICGLIFY
                     TSMSIMESIFPLWANDIFIFGPSKIGAVFFILGFIAVIIQGGLIGPLTSLFGEKKLIQ
                     IAALFLMLGLGLIGSAANTIVLWSGLLFFGLGTSMFNPSISSLISKSANPQERGKFLG
                     QYQSACSMGRVVGPSISGVIYSNFIPAAPFYLGLIIVIPVIVLIANFTLRKIVK"
     gene            complement(1819..>2331)
                     /locus_tag="QGH35_02230"
     CDS             complement(1819..>2331)
                     /locus_tag="QGH35_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009783471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81383.1"
                     /translation="KVNPPIRPREDVEYLWESLLDNKIDWVCSDHACCKHEDKVDIEN
                     PGDIWLAKSGFGGTEYLMSALVSEGSKRGLSFNKMAELTSYNPARRYGLRNKGDIDIG
                     LDADLVLVDPKETWTIDSEKSESGQGYTPFEGQELNSRVTTTFLRGTRIYDNHEIIGQ
                     PSGQYLKRPY"
BASE COUNT          709 a          521 c          445 g          656 t
ORIGIN      
        1 gaagagaaga gcagcagatc agtggcaaca atgccaggag acaatgaccc aatgatcaag
       61 ggggtatctt ggtattcatt tgcaactgca gcagtgaagt aacgcagagc tctttttgtc
      121 gacccataga ccgttatacc cggttgtaat tgtccattac tgccaaatcc ctcaaacatg
      181 tatatctgcc caaatccttg tttcaacatt tctttagcgg ctacttttgt acaaaaaata
      241 ctgccaatta aattggtatc aacagtgtta tgaatttcat ctaattgcaa ccgatctaga
      301 attgtttttg tggtggagac acctgcattg ttaatccaaa tgtcaatttt gccgaaatgc
      361 tcaaaccctt tattccagag tgattgcact gattcataat cctgtacctg gcacggttga
      421 ccgatcattt ctccaagacc atcgagttgt ccaagcgccc gctctacaga gcgtttactg
      481 gttccggaga caactacttg gtggcctcgt tttaaaaatt ctctagccaa tccaagtcca
      541 atgcctcgag tgctgcctgt gatcacaacc gttttcattt tagattccat tcaatgttaa
      601 ttatgatctt attttattca cttaactatc tttcttaaag tgaaattggc aattaagacg
      661 atgaccggta taacaatgat aagacctaaa tagaacgggg cggctggaat aaaattacta
      721 tagatcactc ctgatattga aggtccaaca acacgaccca tggaacaagc ggattgatat
      781 tgacctagaa atttacccct ttcctgtgga ttagcagatt tagagatcag acttgatata
      841 cttggattga acatcgatgt tccaaggcca aagaataaca gtccactcca aagtacaata
      901 gtattcgctg cactgccaat taaacccagt cccagcatta gaaacagtgc tgctatttgg
      961 atcagttttt tctcgccaaa taatgacgtc aatggtccaa ttagcccacc ttgaataatg
     1021 acagcaataa aaccgagtat aaaaaatacg gctccaattt tactgggacc gaaaataaaa
     1081 atgtcgttgg cccacagagg aaaaatagat tccatgatgg acatcgaggt atagaagatc
     1141 agaccacaga tcacaagggg caagattatt ttagaaggct gaaatgtgtc taactctatg
     1201 atctttgaca atccttcaaa tggttttcta ttggcatcat ccaaactttc tttgaggaaa
     1261 aatagagcag caaacatggc gataaaattg atgcctgcag cggataatgc tgtcagtaaa
     1321 taatttgcat tttcaacatt attaccggct agcagcccac caattgctgg tccagtcata
     1381 aatccaagac ccagtcctgc actgactttg cccaacccgg ctgagcggtt ttcatcggtg
     1441 gtgatatcac tgacataagc aaacgcaatt gcaatatttc ctgccatcag accagagaga
     1501 atccgactca ggatcaccac ctccaagtta ggcgcatagg caaggattag gtatgaaatc
     1561 ccagagccaa acaaggtgat gactaaagtt tgcttcctac caatggaatc actcaattgc
     1621 ccccaaatag gggcagcaat aaactgaccg agagaaaaag ctgccattgt gagagtaata
     1681 acttctggac cggcgcccaa gcgttctgca tagaagggaa ataaaggcaa aatgattcca
     1741 aagcccatca tcccaataaa tgtcactaaa aaaattatag ccatttgctg tatgaatttt
     1801 tgttattgaa tgtaactctt agtatggtct ttttaggtat tgtcccgatg gttgaccgat
     1861 aatctcatga ttatcataaa ttcgggtccc ccgaaggaac gttgtagtga ctctcgagtt
     1921 cagttcttga ccttcaaaag gagtataacc ttggccggat tcggactttt cagaatcaat
     1981 ggtccatgtc tctttagggt ctactaaaac caaatctgca tcgagcccaa tgtctatatc
     2041 gcctttatta cgaagaccat atcgtctagc ggggttatag cttgttaatt ctgccatttt
     2101 attgaaagac aaacctctct ttgagccttc actaacgagg gctgacatta aatactctgt
     2161 gcctccaaag cctgattttg ccaaccagat atcacctgga ttttcaatat caaccttgtc
     2221 ttcgtgtttg caacaagcat gatcgctgca aacccagtct attttgttat caagtaaact
     2281 ctcccagaga tattcgacat cctctctcgg cctaatcggc ggattgacct t
//
LOCUS       DASCTH010000107         2794 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000156696, whole genome shotgun sequence.
ACCESSION   DASCTH010000107 DASCTH010000000
VERSION     DASCTH010000107.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000156696"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /gene="parC"
                     /locus_tag="QGH35_02235"
     CDS             complement(<1..1236)
                     /gene="parC"
                     /locus_tag="QGH35_02235"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HJL81384.1"
                     /translation="MGDQKELDFKNTSSASGVGIQPLREFAEKAYLDYSMYVILDRAL
                     PFIGDGLKPVQRRIIYAMSELGLSAKSKPKKSARTVGDVLGKYHPHGDTACYEAMVSM
                     AQNFSYRYPMIIGQGNWGSYDDPKSFAAMRYTEAKLSAYTRLLLSELDQGTVDWKLNF
                     DGTLNEPVYLPAKLPNLILNGVTGIAVGMSTDIPPHNIGEIATLLDKLITDPNQSIGQ
                     LLRSKKFQGPDFPSGGEIITPMSEIKNIYKTGLGTIKIRACYRKEKGNIIIDELPYQV
                     SGNKLIEQIATQMKDKKLPMVSDINDESDHETPIRVVITPKSSRVDHNKLMSHLFATT
                     DLEKTIRVNMNLIGLNGKPKVYDLKNLLLEWLEFRYQTVKKRLECRLSEVEERLHILE
                     GLMIVYLNLDAVIRIVREED"
     gene            complement(1236..>2794)
                     /locus_tag="QGH35_02240"
     CDS             complement(1236..>2794)
                     /locus_tag="QGH35_02240"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013713823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HJL81385.1"
                     /translation="LHGVGVSVVNALSKNLEAWIKRNGHEYNLSFKNGEKISELEVVG
                     NVGKANTGTAIRFWPNSKYFHSNQINVNDLKHNLRAKAVLCPGLKIKLFIEEEDEKLE
                     WLYQGGVNEYLNEELNGAECIPSKPFYGTSRSGKEEIEWSLTWPIDGTIGIQESYVNL
                     IPTMQGGTHVAGFRAGLTDAIRDFAEYRNLLPRGIKLSPEDVWYGISFILSVRLEDPQ
                     FSGQRKEKLSSRSATSYVSGVIKDAFTLLLNQYSEAGDQITARAIENAQQRIKRNKKV
                     KRKKPTGGPMLPGKLADCSSDEPSLCELFIVEGDSAGGSAKQARDRKIQAILPLRGKI
                     LNSWEVDESKLLSSQEISNIALALGVHPGSDNLDNIRYHKICILADADSDGQHIATLI
                     CALFVKHFPELIRAGYIYVAMPPLFRIDVGKEVFYALDENEREELLKTIQKKKIKGKL
                     TVTRFKGLGEMSPTQLRETTMKQTTRRLVQLTIDAEDGTNEMLDMLMSKKRYPDRRKW
                     LENKGNLAEI"
BASE COUNT          750 a          626 c          478 g          940 t
ORIGIN      
        1 gtcttcttct ctcacgatac gtattactgc atcaaggttg agataaacaa tcatcaatcc
       61 ttcgagaata tgaaggcgtt cttcgacttc gcttaagcgg cattcaagtc tttttttaac
      121 cgtctgatat ctgaattcaa gccattctag aagcaaattt tttaaatcgt acacctttgg
      181 tttgccattc aatccaatta aattcatatt gactcgaatt gttttttcca aatccgtggt
      241 tgcaaataaa tggctcatca gtttgttgtg atctaccctt gaagatttag gtgttatcac
      301 cactctgatt ggtgtttcat gatcagattc atcattgatg tcgctcacca ttggtaattt
      361 tttatctttc atttgggtgg caatttgctc gatgagctta ttgccagaaa cttggtatgg
      421 caactcatca ataataatat tgcccttctc ttttctgtaa caagccctga tttttattgt
      481 tcccaagcct gttttataaa tattttttat ttcactcata ggagtaataa tttcgccacc
      541 agaaggaaaa tcaggtccct gaaatttttt actcctgagt aattggccta tagattgatt
      601 tgggtctgta attaatttat ccaaaagtgt ggcaatttct ccgatgttgt gtggaggaat
      661 atcggttgac attccaaccg caataccagt cactccattg agtatcaagt ttggtagttt
      721 ggctggaaga taaactggtt cattgagtgt tccatcaaaa ttaagtttcc aatcgactgt
      781 gccttgatct agttcagata atagaagcct tgtataggca cttaattttg cctcggtata
      841 ccgcatggct gcaaacgatt taggatcatc gtacgatccc caattcccct gtccaataat
      901 cataggataa cggtatgaaa agttttgagc catactgacc atggcctcat aacaggcagt
      961 atctccatgt gggtggtatt tacccaaaac atcccccact gttcttgctg attttttagg
     1021 tttagatttt gcagataagc ccagttctga catagcataa ataatccttc tttgaaccgg
     1081 tttcagacca tcaccgataa acggtaaagc acgatctaat atcacataca tggaataatc
     1141 caaatacgct ttttcagcaa attccctaag gggttgaata cctacaccgg atgcactaga
     1201 tgtattctta aaatccaatt ccttttggtc acccattata tttctgccaa gttgccctta
     1261 ttctcaagcc atttacgtcg atcgggataa cgcttcttag acattagcat atccagcatc
     1321 tcattagtac catcttctgc atcgatcgtt aattggacaa gccttctggt tgtctgtttc
     1381 attgttgttt cacgtaattg agtggggctc atctctccca atcctttaaa tcttgttacg
     1441 gttaatttgc cctttatttt tttcttctga attgttttta gtaactcttc cctttcattc
     1501 tcatctagtg cataaaatac ttctttccca acgtctattc taaacagcgg tggcatcgcc
     1561 acatagatat agccggctcg aatcagttct ggaaaatgtt tcacaaataa agcacaaatt
     1621 aaagtagcga tgtgctgtcc atctgaatcc gcatctgcaa ggatacaaat cttatgataa
     1681 cggatattgt caagattatc tgatcctgga tgaacaccta aggctagggc aatattgctg
     1741 atttcctggg aggataataa cttcgattca tccacttccc aactgttcaa tattttccct
     1801 ctcagtggca gaattgcttg aatttttcga tcacgtgcct gtttggctga gccaccagct
     1861 gaatcacctt ctacaataaa cagttcacac aaactcggtt catcgctcga acaatcagct
     1921 aatttaccag gtaacattgg accgcctgta ggttttttcc tcttaacttt tttgtttctc
     1981 ttaattcgtt gttgagcgtt ctctatggca cgtgctgtga tctgatcacc agcctcagaa
     2041 tactgattta acaacaatgt aaaagcatcc ttgatgacac ctgatacata agaagttgca
     2101 ctccttgagc tcagcttttc tttcctctgc ccagaaaatt ggggatcctc cagtcgtaca
     2161 gagagaataa aactaattcc ataccaaaca tcttcaggag acaacttgat ccctcttgga
     2221 agaagattcc gatactctgc aaaatccctg attgcatcgg tcaacccagc cctgaatcca
     2281 gcaacgtgag ttccgccttg catcgtagga atcaagttta catatgattc ctgaattcct
     2341 attgtgccat ctatgggcca tgtcagactc cattcaatct cttctttgcc cgatctgctg
     2401 gtaccataaa aaggcttaga aggtatgcat tctgcgccgt ttagttcttc attgagatat
     2461 tcattgactc cgccctgata aagccattca agtttttcat cttcctcttc aatgaatagt
     2521 ttaattttta atcccggaca aagaacggcc ttggctctta aattatgctt taaatcattg
     2581 acattaattt gattggaatg gaaatatttt gaattcggcc aaaatcttat ggcagttccc
     2641 gtattcgctt taccaacatt cccaaccact tccaattctg atattttttc accatttttg
     2701 aaactgaggt tatattcatg tccatttcgc ttaatccagg cttcaagatt ttttgaaagt
     2761 gcgttgacta cggacacgcc caccccatgt agcc
//
LOCUS       DASCTH010000108        11804 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000156982, whole genome shotgun sequence.
ACCESSION   DASCTH010000108 DASCTH010000000
VERSION     DASCTH010000108.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000156982"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="QGH35_02245"
     CDS             <1..657
                     /locus_tag="QGH35_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013069.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HJL81386.1"
                     /translation="TTNVVLQTVRKEIAPDIEKRFNVQIDYKGKAEEQAEAFADMSLA
                     LIITFASIYIILAWLFSSYTTPFLVMSVVPFGLIGAIIGHYLMGFNLGMFSLLALLGL
                     TGVLVNDSIILVTTIKRFIADGLDLSVAVLEGTRERLRPVILTTLTTILGLTPILFER
                     SLQAQLVQPLAITFVFGMLFSPFLVLFYLPAVMGIAESFKNNISSLVQQIKPGKRQYS
                     "
     gene            697..1455
                     /locus_tag="QGH35_02250"
     CDS             697..1455
                     /locus_tag="QGH35_02250"
                     /inference="COORDINATES: protein motif:HMM:NF019342.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HJL81387.1"
                     /translation="MSSKNTPVVGITTCHRLNDQHYFHVAGEKYISAVNNFSNCAGIL
                     VPAILQTSINESILDTLDGFLLTGSLSNVHPKRYNEEIIDSNLRLDETRDACVFSLIH
                     SIIERKMPLLAICRGFQEMNIAFGGTLYQDLERDSKYRGHDFDRDEPTDIQYGISHDV
                     AFEHNGLLQRITGAQTAQVNSLHTQGVKQLGDGLSVEATSQDGLIEAFTVNDADGFNL
                     SVQWHPEWQPNTSSISTALFEYFGQACKEFAEQS"
     gene            1452..2789
                     /locus_tag="QGH35_02255"
     CDS             1452..2789
                     /locus_tag="QGH35_02255"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HJL81388.1"
                     /translation="MKQQEIIDWFKQHDIEDIEVIVPDMAGVARGKMLPAEKFLEQPA
                     MRLPESVFVQTVTGEYLHEPLINPADRDILVKPDMDTLRVLPWAKDSTATVINDCYYE
                     DGSAVDISPRQVLRNILKVYQEKNMVPMVAPELEFYLVKRNTDPTVPLEPPIGRSGRQ
                     ETVRQPFSIDALDEFEPVIEDIYQYADSMGIKIDTLEHEMGTAQLEINFEHGEAMQLC
                     DHVIMFKRIVREAAMRHNIYATFMSKPMQAEPGSAMHWHISILDIGSNNNIFSNDEDE
                     ETELFKHFIGGLQTYIPEAILFNAPYVNSYRRFTRWQNAPINIYWGYDNRTTGLRVPS
                     SEPENRRIENRIAGADVNPYLSIAASLACGYLGAQEEIKPSAPEGSNAYNLPFTLPKN
                     LSNAIEMLEHSEAMQMLFGERFLQIYLEIKQYEYQKYFASISPWEREFLLLNV"
     gene            2792..4156
                     /locus_tag="QGH35_02260"
     CDS             2792..4156
                     /locus_tag="QGH35_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HJL81389.1"
                     /translation="MTKPIDQLKNLDRNHYLHPFTDHHLLSKKGTRIITKGEGIYIWD
                     AEGNKILDAMSGLWCVNVGYGRTELIEAAYEQMQELPYYNSFFHCAHPPAIELGELLS
                     QISPGDLNHVFYTGSGSEANDTIVRMVRRYWQLLDKPDKQIILARNNAYHGSTVAAAS
                     LGGMTGMHAQGGLPIPNIHHIAQPYWYGEGADLDPDSFGIKVAQELQLAIDQFGIDNI
                     AAFIAEPIQGAGGVIIPPETYWPAIQSICDDHEILLIADEVITGFGRLGEWFGSKVYQ
                     IKPDLIPFAKGVTSGYLPLGGVLVNDKVADTIISKGGEFTHGFTYSGHPAACAVAIAN
                     IKVLHQDNIIDQVRQETAPYFNKLWQTLADHPIVGEARAKGLVAAIEICKDKSKRERF
                     DIDPSAGSLCRDFAIKNGIVMRAVGDTMIVAPPLIISIEQIDELISKVGDTLDETLAA
                     VGAI"
     gene            4180..5466
                     /locus_tag="QGH35_02265"
     CDS             4180..5466
                     /locus_tag="QGH35_02265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJL81390.1"
                     /translation="MENKPDHPNSYYVATAKELREYPQLSESIEADVCIIGGGFTGLL
                     AALNLAEKGYQVVLLEACRIGWGASGRNGGQVGSGYNKKIPELERHYGTSLAKDLWNL
                     TEDAKSIVKDRITQHDIDCDLSFGNVTVSNDQRDSESDREYVDKLNRDYNYNEIRYLD
                     EAEVHEMFHSPLFKGGGSLDLGGMHLHPLNFMLGLAKAATKAGVQIFEQSRVIQYTKL
                     KPSVIRTEQGQVMAKHIVLACNAYLEKLEKRIAGKMMPINNFILATEPLEESEARFIN
                     RDNVCAHDKKFHVHYFRMSKDHRLIFGGGENYTTRIPKDLKAYVRKTMLNVFPSLESK
                     IIDYAWSGSVAVTVNRMPHLGRLKPNVFFAHGFSGHGIALASLAGTLIAEAIDGTVSR
                     FDVFSKIKIPTFPGGTMLRWPGFYLGMIYSSIRDRL"
     gene            5463..6560
                     /locus_tag="QGH35_02270"
     CDS             5463..6560
                     /locus_tag="QGH35_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003849278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HJL81391.1"
                     /translation="MIRLKLLAIKISLFLIIFSPFSHAQEVRVYNWSDYIDDSIIENF
                     EKETGIDVIYDVFDSNEVLEGKLLAGNTGYDIVAPSIEYLGRQIVAGVFQKLDRDQLP
                     NYQNLDTEMLALLSNMDPGNKHGIPYMWGTTGIGYNKQKAIEIMGGNYPMNSFDIVFK
                     PEIISKFEDCGVAFLDAPSETFKAALYYVGLDPNTKNPDDYRGESFKVLKSVRPYIKY
                     FHSSKYINDLANGDLCLVFGWSGDILQASDRARESGNGIEIGYMIPVEGAQMWFDMMA
                     IPKDAPNPENAHRFLNYILQPEVMAIISNKIKFANANIKSKPLINKEITNNPSIYPPD
                     EILNKLFVAEIATPKVDRTMTRQWINIKTDR"
     gene            6570..8279
                     /locus_tag="QGH35_02275"
     CDS             6570..8279
                     /locus_tag="QGH35_02275"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81392.1"
                     /translation="MNIKNIIIIVALSQTILSSCERFVDQEHADSVFLDGAIYTFDQN
                     LSTIEAVAVRGGMVIARGTNEEINEWIGENTEVYPLDGKMMIPAFHDAHAHPIWSGID
                     QLKCPVFNLPSIPAIQQAVKECLNSKMTKSTGWVAGAGYNLGLFPAGNADKSLLDAVS
                     LEVPIFLDGEDGHSGWANSKALELAGISADTPNPELGIIEKDPVTGEPTGTLREPAAI
                     SLIKKIIPGDDLELFDDGLLFAQDLAHSFGITSIIAASVGEQHLAAYKRLADQGGLDL
                     RMTTSIEYGNTGFTLGPGGFEEVFSKRQQYQHPRINVDNIKIFIDGVLEGQTAALLSP
                     YLNTGSIGELNIQQAELDEAVTRFDREGIQVHMHAIGDQGVRTGLNAFEAALVVNGPT
                     DNRHHIAHLQLISEADIPRFAELKVIANFQPVWGYPELYITEINLPEVGPERVNRMYP
                     IASVHRAGGMVVGGSDWAVSTMNPLVAIETAIRREDPENLIKGVLNEDERMNLTEMLK
                     AYTINSAYLMHQEHLTGSIEVGKAADLIILEKNLYTIPVDEISEVKVLQTMIEGKTVY
                     TAN"
     gene            8282..10264
                     /locus_tag="QGH35_02280"
     CDS             8282..10264
                     /locus_tag="QGH35_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013739.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HJL81393.1"
                     /translation="MTHNNDGITRRDFLNGVSLGIAGSLAPIDLLYTADTPLVYYPPT
                     LTGIRGNHPGSFDYAHKLAFGGSDFINEVEDLGKSYDLIVVGGGISGLSAAHFFKERT
                     EKIPKVLILDNHDDFGGHAKRNEFVVNNRMMLAYGGSQSIESPRYYEPVSKKLLTDLG
                     IDFEKFYTAYDFDYFKNRKLDTGFYFNKATYGENRIVKNVPSFRYDLTYKESIKPDNI
                     QRVASQMPISDQSKEEFIRLFLDQTDFFPELSLEDKYYLLDNISYEDYLRKYHKVGEE
                     VIGLFHSLVWGLWGVGNDSIPAAGCWGDGLPGFSGLGFMDNEDSIDEINDQENLMYEN
                     ETFDESIRDYMSKNELSNEPYIFHFPDGNATIARLLVRKLIPNSIPGNNMEDIVTAKA
                     DYSMLDKPGQDTNIRLNSTVVSVINKKNGVEIIYAKQGALYKVHAKQCILACYHGTIP
                     ELCPQLPEKQKEALKYNVKVPMVWVQVAMKNWHMLANKGVHTLQCPNCFYNSFYVDFP
                     VSLGDYQFPQTFEDPVVFMMQHVPTRPNQGYTNREQFRLGRHEILTMSYQDYEDKLFD
                     QLRGMFGSDFDEDDVAAITVNRWAHGYSYEYNNLFDAEFFGGELPDDLDDERYPHVIG
                     RKPFGNITIANSDSLGSAYVDAAISQADRAVRELFD"
     gene            complement(10271..>11804)
                     /locus_tag="QGH35_02285"
     CDS             complement(10271..>11804)
                     /locus_tag="QGH35_02285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HJL81394.1"
                     /translation="DADGNVVPLNLLKDDPDHILVMRNDRKRYLYDIYKMNVFTGNFF
                     REISPPGPVVSWGADHEGVVRLAAMQDENSLSYETQIIYRENEDSEWREVDRFEGLQN
                     GWGSLGFTEDNKKIYITSNLGRDTYSIGLFDPETGDIEDLFENEGTDVTRLGFSDDGE
                     AITIVYNDINTKPKRHFINEKWKNIIENLNVSFGVDFVGITSMSDDQQKMILSIADDR
                     NQGDYYLYDRGKNTITYLGSVRPWIDPKTMAEMKPISFTARDGETVHGFLSVPVDSNG
                     KNLPLIINPHGGPFGIQDQWRFNGETQFFVNRGYAVMQINFRGSGGYGKRFERIGFKR
                     WGLEMQDDISDGVYWAVEQGIADPEHVCIYGASYGGYATMAGITLTPELYRCAVNYVG
                     IWDIKMLYEQTGRWTDRAKRWFHNHVLDPKKDLDQLKKTSPNFHLDKIKAPLFIVHGR
                     RDYNVRVEQAETLMDALDEKNIPYEVLIKKEEGHGFTLEENKIELYSRMEEFFEKNLA
                     KY"
BASE COUNT         3572 a         2258 c         2551 g         3423 t
ORIGIN      
        1 accactaatg tggttttgca aacagtcaga aaagaaattg ctccagacat tgagaaacgt
       61 ttcaatgtac aaatcgatta caaagggaaa gctgaagaac aagctgaagc ctttgctgat
      121 atgagcttag ctttgatcat tacatttgca tcgatataca ttattctggc ttggttgttt
      181 tctagttaca caaccccgtt cttggtgatg tcagttgtgc cttttggctt aatcggagcc
      241 attattgggc attatctgat ggggtttaat ctgggtatgt ttagtttgct agcactttta
      301 ggtttaactg gtgtattggt aaatgattcg atcatattgg tgacgaccat taagcggttt
      361 attgccgatg gactcgattt aagtgtagca gtgttagagg ggacaagaga gagactgaga
      421 ccagttattt taaccacctt gacaacaatt ctggggttaa ctccaatact ttttgagcga
      481 agtttgcaag ctcaattggt tcagcccttg gccataacct ttgtttttgg aatgttattt
      541 tctccttttt tggtgctatt ttatcttcct gcagtgatgg gtattgcgga gagttttaaa
      601 aataatatca gctcgctagt acagcaaatc aaacctggta aaagacaata tagttagcca
      661 tatctttctt gcatcagtag aataaatgat cattctatga gctcaaaaaa tacacctgta
      721 gtaggcatta caacctgtca tcgtttaaac gatcaacatt attttcatgt ggcgggagaa
      781 aaatacatat cagcggtcaa taatttttct aattgtgcag gaattctggt gcctgcaatc
      841 ttacaaacga gcattaatga gagtattcta gatacattgg atggtttttt gcttactggc
      901 agtttgtcta atgttcatcc aaaaaggtat aacgaagaga tcattgattc gaacctaaga
      961 ctcgatgaaa ctagggatgc atgtgtgttt agtctcattc attcaatcat tgagagaaaa
     1021 atgcctttat tagccatttg ccgtggtttc caagagatga acatcgcatt tggtggcaca
     1081 ctatatcaag atcttgaacg cgattctaaa tatagaggcc atgattttga tagagacgaa
     1141 cccacggata tccaatatgg aatcagtcat gatgtagcat tcgagcataa cggtttgttg
     1201 caaaggataa caggagctca aaccgcccaa gtcaattcgc ttcataccca aggtgtcaaa
     1261 caattgggag atgggttgag tgttgaagcc acgagccaag atggcttgat tgaagcgttc
     1321 actgtcaatg atgctgatgg gtttaactta tccgttcagt ggcacccaga atggcaaccc
     1381 aacacaagtt ctatttcaac tgctttattt gaatattttg gacaagcgtg caaagaattt
     1441 gccgagcaat catgaaacaa caagaaatta ttgattggtt taagcagcat gatattgaag
     1501 acattgaggt gatagtgccg gatatggcag gtgtggcacg tggtaaaatg ctgcctgctg
     1561 agaaatttct agaacaacca gcgatgcgcc tgcctgaatc ggtctttgtc caaacggtaa
     1621 caggtgaata tctccatgaa ccccttatta acccagcaga cagagatatt cttgtcaaac
     1681 ctgacatgga tacgttaaga gttttgccgt gggcaaaaga ttccacagca actgtcatta
     1741 atgactgcta ttacgaagat ggctcagctg ttgatatctc accaagacaa gttttgagaa
     1801 atatactaaa agtgtatcag gaaaaaaata tggtgcccat ggtggctcca gagctggaat
     1861 tttatttagt caaacgcaac actgatccaa ctgttccatt ggagccgccc atcggtcgtt
     1921 caggaaggca agaaacggtt cgccaaccgt ttagtattga tgcgcttgat gaatttgaac
     1981 cggtgattga agacatttat cagtatgcag atagcatggg gattaagatt gataccctgg
     2041 aacatgaaat gggaacggct cagttggaga tcaattttga acatggtgaa gctatgcaac
     2101 tttgtgatca tgtgattatg tttaaacgga tcgtgagaga ggctgcaatg agacacaata
     2161 tctatgcaac ttttatgtct aagcccatgc aggcagaacc tggaagcgcg atgcactggc
     2221 acatttcaat tctagatatc ggatccaata ataatatttt ctcaaatgat gaagacgaag
     2281 agacggaact gtttaaacac ttcataggtg gtttgcaaac ctatattcca gaagcaatac
     2341 tgtttaatgc accctatgtt aattcttacc ggcgttttac gcgttggcag aatgctccta
     2401 ttaatatcta ctggggctat gataaccgaa ccacgggact cagggttcct tcatctgaac
     2461 cggagaatcg tagaattgaa aataggattg ctggagcaga cgtgaaccct tacctatcga
     2521 ttgcagcttc tttggcatgc gggtatctgg gagcacaaga ggagataaaa cctagtgcgc
     2581 cagagggctc taacgcctat aatctaccct ttacacttcc aaaaaatttg tccaatgcta
     2641 tagaaatgct ggaacacagc gaagcaatgc aaatgttatt tggagagcgt tttttacaga
     2701 tttatttaga gatcaaacaa tatgaatatc aaaaatattt tgcaagcatt agcccttggg
     2761 aacgagagtt tttattatta aacgtgtaat catgactaaa cccattgacc aattaaaaaa
     2821 tctggatcgt aatcattatt tgcatccatt tacagatcat catcttctgt ccaaaaaagg
     2881 cactagaatc attaccaaag gcgaagggat atatatatgg gacgcagagg gcaacaaaat
     2941 tttggatgca atgtctggat tatggtgtgt gaatgtcggt tatggcagaa ccgaactgat
     3001 tgaagctgcc tatgagcaaa tgcaagaact accctactat aatagttttt tccactgtgc
     3061 tcatccacct gccattgaac ttggagagct attgagccaa atttcacccg gtgatttaaa
     3121 tcatgttttc tataccggat cagggtctga ggccaacgat accatcgtga gaatggttcg
     3181 cagatattgg caactactgg acaaacccga taaacaaatt attcttgcca gaaacaatgc
     3241 ttatcatggc agtacagttg ctgctgcaag tcttggcggt atgacaggaa tgcatgcaca
     3301 gggtgggtta cccattccaa atatacatca tattgcccaa ccctattggt atggagaagg
     3361 ggcggatctt gatccagact cctttggaat caaggttgcc caagagctac aattagccat
     3421 cgatcagttt gggattgata atattgcagc atttatagct gagcccatcc agggagccgg
     3481 tggtgttatt attcctccag aaacttattg gcctgcaatc cagagtatat gcgatgatca
     3541 tgaaatttta ttgatcgccg atgaagtgat aaccggcttc ggtcgattgg gagaatggtt
     3601 tggcagcaaa gtctaccaga ttaagccaga tctcattcca tttgccaaag gagtcacttc
     3661 gggatatctc cctttagggg gcgtattagt gaacgacaaa gtggctgata ccattatctc
     3721 caaagggggt gaatttaccc atgggtttac ttattctgga catcctgccg cctgtgctgt
     3781 agccattgca aacataaaag tcttacatca agacaacatc attgatcaag tcagacaaga
     3841 gacggctcct tatttcaata aactgtggca aacacttgca gaccatccaa ttgttggcga
     3901 agccagagcg aaaggactag ttgctgcaat tgaaatctgc aaagataaat cgaagcgaga
     3961 acgatttgat attgacccaa gtgcaggttc gctgtgtaga gattttgcta taaagaatgg
     4021 cattgttatg cgtgctgtag gggacaccat gattgtggct cctcctttga ttatcagcat
     4081 agagcagatt gatgaattga ttagcaaagt gggcgacacg ctggatgaaa ccctggcagc
     4141 agttggagca atataactct aatttttggt ctacaatcaa tggaaaataa gccagatcat
     4201 cctaattcct actatgttgc aacagcaaaa gaattaaggg aatatcctca attaagcgaa
     4261 tctattgaag cggatgtatg catcattggg ggaggtttta caggactgct tgcagccctt
     4321 aatctagccg aaaaaggtta tcaggtggtc ttgttagagg cttgtagaat tggctgggga
     4381 gcatccggta gaaatggtgg tcaggtgggt agcggctata ataaaaaaat acccgaactt
     4441 gagaggcact atggaacatc ccttgctaaa gatctctgga atcttactga agacgcaaaa
     4501 tcgattgtca aagacagaat cacacaacat gatattgatt gcgatctatc ttttggaaat
     4561 gtaaccgttt ctaacgacca aagagattcg gaatcagaca gagagtatgt agataaatta
     4621 aatcgtgact acaactataa tgaaatcaga tatcttgatg aagcagaagt ccatgagatg
     4681 tttcacagtc cactttttaa aggaggtgga tcgctggatc tgggtggcat gcatctacac
     4741 cctctgaatt tcatgctcgg tctagccaag gctgcgacca aagcgggcgt acagattttt
     4801 gaacaatctc gcgtgatcca atacacaaaa ctcaagcctt cggtcatccg aacagaacaa
     4861 ggtcaggtta tggccaagca tattgttctg gcatgtaatg cttatctgga aaaattagaa
     4921 aaaagaattg ccggaaagat gatgccaatc aataatttta tactggccac agaaccactt
     4981 gaagaatcag aggcacggtt tatcaatagg gataatgttt gtgctcatga taagaaattt
     5041 catgtgcact attttagaat gtccaaagat catcgtctca tatttggtgg cggtgaaaat
     5101 tatacgacaa ggattccaaa ggatttaaaa gcttatgtac gcaaaactat gctcaatgta
     5161 tttcccagtc tagagagtaa aatcatagat tatgcctgga gtggttctgt tgcggtgacg
     5221 gtcaacagaa tgcctcattt aggtcgttta aagcccaatg ttttttttgc acacgggttt
     5281 tcaggacatg gaatcgccct agcctcatta gctggcactt tgattgcaga ggcaatcgat
     5341 ggaacagtaa gtcgctttga tgtattcagt aaaattaaaa taccaacctt tcctgggggc
     5401 actatgctga ggtggcctgg cttctatctg ggtatgatat attcttcgat acgggataga
     5461 ttatgattag attaaaactc ttagcaatta aaatttcttt atttttaata attttctcac
     5521 ctttcagtca tgctcaagaa gtaagagtct acaattggtc tgattacatt gatgactcca
     5581 tcattgaaaa ctttgagaaa gaaactggca ttgatgtgat ctacgatgtc tttgacagca
     5641 atgaggtttt agaaggtaaa ttactggctg gaaatacagg ttatgacatt gtagccccca
     5701 gtattgagta tttgggcaga caaattgttg caggtgtctt tcagaaacta gatcgagatc
     5761 aattgccaaa ttatcagaat ctggatacag aaatgcttgc attactatct aatatggacc
     5821 caggaaacaa gcacggaatt ccatatatgt ggggcaccac aggcattggt tacaacaaac
     5881 aaaaagcgat agaaatcatg ggaggaaatt atcccatgaa ctcatttgat atcgttttta
     5941 agccagaaat tatcagtaag tttgaagatt gcggtgttgc cttcctggat gcaccaagcg
     6001 agacatttaa agcagcactg tactatgttg gtctcgatcc caatacaaaa aatccagatg
     6061 actacagagg tgaatcattc aaggtgttga aaagtgtccg cccttatata aaatattttc
     6121 attcttcaaa atatatcaac gatcttgcaa atggagatct ttgtcttgtg tttggatggt
     6181 cgggagatat tttacaggca tcagaccgtg cgagagaatc tggtaatggc atagagatcg
     6241 gatatatgat tcctgtcgag ggagctcaaa tgtggtttga tatgatggcc attccaaaag
     6301 atgcgcctaa tcctgaaaat gcacatcgtt ttctcaatta cattctacaa cccgaggtca
     6361 tggctattat tagcaataaa attaaattcg caaatgcaaa tattaaatct aagccattga
     6421 ttaataaaga aataacaaac aatccaagta tttacccacc tgatgaaata ctgaataaat
     6481 tgtttgtcgc tgaaattgca actcccaagg tagatcggac catgaccaga cagtggatta
     6541 atataaaaac agataggtaa aaaagatcca tgaatattaa gaacattatt atcatcgttg
     6601 cactttctca aacaattctt tcatcgtgtg aaagatttgt agatcaggag catgctgact
     6661 cagttttttt ggatggagca atctatacat ttgaccagaa tctttcaacc attgaagcag
     6721 ttgcagtcag aggcggaatg gttattgcta gaggaactaa tgaagaaatc aatgaatgga
     6781 ttggtgagaa cactgaagtt tatccattgg atggaaaaat gatgatacct gcttttcatg
     6841 atgctcatgc acatccaatc tggtctggta ttgatcagct caaatgccct gtttttaatt
     6901 taccaagcat cccagccatt caacaagcag tgaaagagtg tcttaatagt aagatgacta
     6961 agagtacagg atgggttgca ggtgctgggt ataatcttgg gttatttcca gcgggcaacg
     7021 cagataaatc tcttttggat gctgtcagtt tagaggtccc catattcttg gacggtgaag
     7081 atggtcattc aggttgggca aattctaaag cacttgaatt ggcaggcatt agtgcagaca
     7141 ctccgaaccc agaattggga attattgaaa aagatcccgt tactggagag ccaaccggaa
     7201 cactcagaga gccggcagca ataagcctta ttaagaaaat tatacctgga gatgatttag
     7261 agttatttga tgatggactt ttatttgctc aagatttagc acacagtttt ggtattacct
     7321 caataatagc tgcaagtgtg ggagagcaac atttggctgc gtacaagaga ctggcagatc
     7381 aagggggtct tgatttaaga atgaccacga gcattgaata tgggaacact ggatttaccc
     7441 taggacctgg gggatttgaa gaggtatttt ccaagcggca gcaataccaa catccgagaa
     7501 ttaatgtaga caatatcaaa atttttatcg atggggttct agagggtcaa acagcggcat
     7561 tactgtcgcc ttatttgaac acaggtagca ttggagagtt gaatatacaa caggcggagc
     7621 tggatgaggc agtaacgcga tttgaccgag agggcattca agtgcacatg catgcgattg
     7681 gtgatcaggg tgttcgcact ggcctgaatg catttgaagc agcgcttgtt gtcaacggtc
     7741 caactgataa tcgccatcat attgcacatc tgcaattaat cagtgaagca gatattccta
     7801 gatttgcaga gttgaaggtc attgcaaact tccaaccggt ctggggctat cctgagttat
     7861 acataacgga aattaactta cctgaagttg gacctgagag agtcaatcgg atgtacccca
     7921 ttgctagcgt tcatcgtgct ggtggcatgg ttgtgggagg cagtgattgg gcagtttcca
     7981 ccatgaatcc tttggttgca attgaaacag ccattagaag agaagatcct gaaaatctca
     8041 tcaagggagt tctaaatgaa gatgagagga tgaatcttac tgagatgctc aaagcgtata
     8101 caattaattc tgcatatcta atgcatcaag aacatctcac cggatccata gaagtgggca
     8161 aagcagcaga cctgattatt ttagaaaaga atctgtacac catcccggtt gatgaaatta
     8221 gcgaagtgaa agtattacaa accatgatcg agggcaaaac agtttatact gccaattaat
     8281 catgactcat aacaatgatg gcattaccag aagggatttt ttaaacgggg tcagccttgg
     8341 gattgcaggt tcgctagctc cgattgattt gctgtacaca gcagatactc cattagtata
     8401 ttatccacca actctgactg gcatcagagg caatcatccg ggttcttttg attacgccca
     8461 caaacttgct tttggaggta gtgattttat taatgaggta gaagatttgg gtaagagcta
     8521 tgatttgatt gtggtaggtg ggggtatttc aggactgtct gctgctcatt tttttaaaga
     8581 gcgtaccgaa aaaattccga aagttctcat actggataac catgatgatt ttggaggtca
     8641 tgccaaacgt aacgaatttg ttgtgaataa ccggatgatg cttgcctatg ggggcagtca
     8701 gtctatagag tcaccgagat attacgagcc agtttcaaaa aaattgttaa cagatcttgg
     8761 tatagatttt gaaaaatttt atacggcata cgattttgac tatttcaaga atagaaagct
     8821 tgatacaggc ttttatttta acaaagcaac ctatggagag aatcggatcg ttaaaaatgt
     8881 acccagtttt agatacgatc ttacttataa agaatccata aagcctgata atattcaaag
     8941 agttgcatct cagatgccaa tttcagatca atccaaagaa gaatttatta ggctattttt
     9001 agatcagact gattttttcc ctgaattaag tctcgaggat aaatattatt tgttagacaa
     9061 catcagttac gaagattatt taaggaaata ccataaagtt ggagaggagg taattggtct
     9121 ttttcatagt ctagtatggg gtctatgggg agtaggtaat gattccattc ctgctgctgg
     9181 ttgctggggt gacggattgc caggtttttc cgggttaggt tttatggata acgaagattc
     9241 aatcgatgag atcaatgatc aggaaaatct catgtacgaa aacgaaactt tcgatgagag
     9301 cattcgtgac tacatgagta aaaacgaatt atccaatgag ccatatattt ttcactttcc
     9361 cgatggcaat gcaacaattg cacgattact ggtgcggaaa ctcattccca attcaatacc
     9421 tgggaacaac atggaagata ttgttactgc taaagctgac tattctatgt tggataagcc
     9481 tggacaagac actaacatcc gtttaaacag taccgtggta tcagtcatca acaaaaaaaa
     9541 tggagttgaa ataatttatg caaaacaagg agcactatat aaggttcatg ccaagcaatg
     9601 cattcttgct tgttatcatg gcaccattcc cgagctttgc cctcaactac cagagaaaca
     9661 gaaggaggcc cttaaataca atgtaaaagt gcccatggtt tgggtgcaag tggcgatgaa
     9721 gaattggcac atgcttgcaa acaaaggagt ccataccctg cagtgtccaa attgttttta
     9781 taatagcttc tatgttgatt tcccagtgag cctcggagac tatcaatttc cgcagacttt
     9841 tgaagatcca gtagtattca tgatgcaaca tgtacctact cgaccaaacc agggctatac
     9901 taacagagag caattccgtc tcggtcgtca cgagatctta acaatgagtt atcaagatta
     9961 tgaagacaaa ttatttgatc aactgagggg tatgtttggt tcagactttg atgaagatga
    10021 cgttgcagcc atcaccgtca accgatgggc gcatggctat tcctatgagt acaacaattt
    10081 atttgatgca gagttctttg gcggtgagct gccagacgat ctggacgatg agcgctaccc
    10141 acacgtgatt ggcagaaaac catttggcaa tattaccatt gccaacagtg actcgttagg
    10201 ctctgcctat gtagatgctg ctatcagcca agctgatcga gcagtacggg aattatttga
    10261 ctaatctgat ctagtatttt gctaggtttt tttcgaagaa ctcctccatg cgactgtaga
    10321 gttcaatttt attttcctct agcgtgaatc catgtccctc ttctttttta atcaagactt
    10381 catagggtat gtttttctca tccaatgcat ccatcaaagt ttcagcctgt tcaactctaa
    10441 cgttatagtc tcgtctgcca tgaactatga atagtggcgc cttgatttta tccaggtgaa
    10501 agtttgggga ggttttcttt aactgatcaa ggtccttttt tggatcgagg acatgattat
    10561 gaaaccatct tttcgctcta tcagtccatc taccggtttg ctcgtacagc atctttatat
    10621 cccagattcc aacataatta accgcgcatc gataaagctc cggtgtcaat gtaatccccg
    10681 ccatggtggc ataacctcca tagcttgcac cataaatgca tacatgctct ggatccgcaa
    10741 taccttgttc cactgcccaa taaacaccat cactgatatc atcctgcatt tccagacccc
    10801 atcgcttgaa tccgattctt tcaaagcgtt taccgtagcc tcctgagccc ctgaaattaa
    10861 tctgcatgac tgcatagcct ctgttcacaa aaaattgtgt ttcaccgttg aacctccatt
    10921 gatcctggat gccaaaaggc ccaccatgtg ggttaataat cagtggtaga ttcttgccat
    10981 tgctatcaac aggcacgctt aaaaaaccat ggactgtttc cccgtccctg gctgtgaatg
    11041 agatgggttt catttctgcc atggttttag gatcaatcca gggtcttact gaacccaaat
    11101 aggtaatcgt attcttgcct cggtcataga ggtaatagtc accttgattt ctatcatcag
    11161 caattgagag gatcattttt tgttggtcat cgctcatgga tgtgatccca acaaaatcaa
    11221 cgccaaatga aacattgagg ttctcaataa tatttttcca tttttcatta atgaaatgcc
    11281 gttttggctt ggtgtttata tcgttataga caatggtgat ggcctcccca tcgtcgctga
    11341 agcctaaccg agtaacatct gtgccttcat tttcaaatag gtcctcaatg tcgccagtct
    11401 caggatcaaa taaaccaata gaataggtat cacgacccag atttgaagta atgtatattt
    11461 ttttattgtc ttctgtaaaa ccaagcgagc cccaaccatt ttgtaaccct tcaaaacgat
    11521 caacctctcg ccattccgag tcctcatttt ctctatagat aatctgggtt tcatagctga
    11581 gagagttttc atcctgcatt gcagctaatc ggactactcc ctcatgatct gctccccatg
    11641 atacaactgg cccaggaggt gagatctctc gaaaaaaatt tccggtaaaa acattcattt
    11701 tataaatgtc ataaaggtat cttttccgat catttctcat tactaagatg tgatctggat
    11761 catctttgag aagatttaat ggaacaacat tcccatcagc atct
//
LOCUS       DASCTH010000109         2275 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000157651, whole genome shotgun sequence.
ACCESSION   DASCTH010000109 DASCTH010000000
VERSION     DASCTH010000109.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000157651"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="QGH35_02290"
     CDS             <1..204
                     /locus_tag="QGH35_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013024.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HJL81395.1"
                     /translation="RLNDLAKVKADVVDLDALKNANIVSRSTENIKVIATGEINKAIT
                     IHGLNVTPRAKEAIINAGGKVMD"
     gene            207..1550
                     /gene="secY"
                     /locus_tag="QGH35_02295"
     CDS             207..1550
                     /gene="secY"
                     /locus_tag="QGH35_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018994260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HJL81396.1"
                     /translation="MARKNIMSPSSSLGEMTRFSELRQRLFFLVGALVVYRIGTYIPV
                     PGIDPNAMQNFFSDQSGTILRMFNMFSGGALERLSIFALGIMPYISCSIIMQMASVTV
                     PSLKQLKKEGETGRKKITQITRYGTVVLSAFQSVGASVALQNSGVVVSPGFNFVFTAC
                     ITLVTGTMFLMWLGEQITERGLGNGISMIILASIISGLPSAIGGTLELANTGEISPAL
                     VLILLMLILAVTTFVVFIERGQRRIPVNYAKRQQGRKLYQSQSTHLPFKINMSGVIPP
                     IFASSIILFPATIASWFGTSEGFDWLQNIASQLSPGQPIYIFVYSSLIMFFCFFYTAL
                     VFDSKETAENLKRSGAFLPGIRPGHQTAQYVDKVLGRLTFWGGLYIMLVCLIPEFMIL
                     YFRVPFYFGGTSLLIIVVVSMDFMAQLQALMMSQQYEGLLKKANLKGYGKSGQVR"
     gene            1561..1674
                     /gene="rpmJ"
                     /locus_tag="QGH35_02300"
     CDS             1561..1674
                     /gene="rpmJ"
                     /locus_tag="QGH35_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HJL81397.1"
                     /translation="MKVRASVKKICRNCKVIRRNGVVRVICKNPRHKQRQG"
     gene            1682..2038
                     /gene="rpsM"
                     /locus_tag="QGH35_02305"
     CDS             1682..2038
                     /gene="rpsM"
                     /locus_tag="QGH35_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HJL81398.1"
                     /translation="MARIAGVNIPDQKHVSTSLTYIYGIGRSQAKTICTKLNILPDTN
                     VSDLSEPELEALRIEITNLTVEGDLRREITTNIKRLLDLGCYRGIRHRRGLPVRGQKS
                     KTNARTRKGPKKPMRA"
     gene            2056..>2275
                     /gene="rpsK"
                     /locus_tag="QGH35_02310"
     CDS             2056..>2275
                     /gene="rpsK"
                     /locus_tag="QGH35_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HJL81399.1"
                     /translation="MAKNPKKKIKKNVNDGIAHIHATFNNTIVTITDRAGNALSWATA
                     GGSGFRGSRKSTPFAAQVAAEKAGKAAQE"
BASE COUNT          763 a          363 c          484 g          665 t
ORIGIN      
        1 cgtcttaatg atttagcaaa agtgaaagca gatgttgtgg atttagatgc attgaagaat
       61 gcaaatattg tttccagatc tacagaaaat atcaaagtga ttgcaaccgg tgaaattaat
      121 aaagcaataa cgatacatgg actaaatgta acccctagag caaaagaagc aattataaat
      181 gctggcggta aggtgatgga ttagaaatgg ctagaaaaaa tataatgagc ccatcatctt
      241 cattaggaga aatgacaaga tttagtgaat tacgtcagcg tctttttttc ctagtgggtg
      301 ctttggtggt ttataggata ggaacatata ttcctgtgcc tggtattgat cctaatgcta
      361 tgcagaattt tttcagtgat cagtctggaa caatcctcag aatgttcaat atgttttcag
      421 gaggtgcctt ggagcgacta agtatctttg cattaggaat tatgccctat atttcctgtt
      481 ccatcattat gcaaatggca agcgtaactg ttccttcact gaaacaatta aaaaaagaag
      541 gcgagacggg cagaaagaaa attacacaaa ttacaagata tgggactgtt gttttatcag
      601 catttcaatc agttggggca tcagttgctc ttcagaattc aggtgtagtt gtttctccgg
      661 gatttaattt tgtttttaca gcttgcatta ctttggttac tggaacgatg tttctaatgt
      721 ggttgggtga acagattaca gaaagaggtc ttggtaatgg tatttcaatg attattttgg
      781 ccagtataat ctctggactt ccctccgcaa ttggaggaac acttgaattg gcgaatacag
      841 gtgaaatatc tccagcattg gtcttaattt tactcatgtt aattcttgca gtaacgactt
      901 ttgttgtctt tatagaaaga ggacagagga gaatacctgt caattatgca aaaagacaac
      961 aaggtagaaa attgtatcaa agtcaatcga cacacctgcc ttttaagatc aatatgtctg
     1021 gtgttattcc accaatattt gcctccagta tcatcttatt tccagcaact attgctagtt
     1081 ggtttggaac atcagaaggt tttgattggt tacaaaatat tgcttctcaa ttatcaccag
     1141 gacaaccaat ttatattttc gtgtattcat cactgattat gtttttctgt tttttttaca
     1201 cagctttggt ttttgactca aaagaaacag cagagaattt aaagcgatca ggcgcatttt
     1261 tgccaggaat tagacctgga catcaaacag cacaatatgt ggataaagtg ctaggaaggt
     1321 taacattttg gggtggattg tatatcatgc tcgtctgcct aataccagaa tttatgattc
     1381 tctactttag agtcccattt tattttggtg gaacgtcatt attaattatt gtagttgttt
     1441 cgatggattt tatggcacaa ctgcaagcat taatgatgtc acagcagtat gagggtctat
     1501 taaaaaaagc gaatctcaaa ggatatggaa aaagtggaca agtaaggtaa tgaagcagtt
     1561 atgaaagtaa gagcatcagt aaagaaaatt tgtcgaaact gcaaagtgat tcgaagaaat
     1621 ggagttgtta gggtgatctg taaaaaccca cgacataaac aaagacaagg ataaatctat
     1681 tatggcacgg attgcaggag taaatattcc agatcaaaaa catgtcagta cttcattgac
     1741 atatatttat ggtattggcc gatcccaagc taaaaccata tgcactaaat tgaatatatt
     1801 acctgatacc aatgtgagtg atttatctga gccggaattg gaagcgctga ggatagaaat
     1861 aacaaactta actgtagagg gtgatcttag gcgggaaatc acaacgaaca taaagcgttt
     1921 attggattta ggatgttacc gcggtatcag acatcgaaga ggcctgcctg ttcgtggaca
     1981 aaaaagtaaa acaaatgcta gaacaaggaa aggccctaaa aagccgatga gggcataaaa
     2041 aaaggaaacc gattaatggc aaaaaaccca aagaaaaaaa taaaaaagaa cgttaacgat
     2101 ggcattgctc atattcatgc aacatttaat aataccatcg tgacaattac agatagagca
     2161 ggtaacgctt tatcttgggc aacagcagga ggctcaggct ttagaggctc aaggaagagc
     2221 actccatttg ctgctcaagt tgcagcagaa aaggcaggga aagctgcaca ggaat
//
LOCUS       DASCTH010000110         3539 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000158121, whole genome shotgun sequence.
ACCESSION   DASCTH010000110 DASCTH010000000
VERSION     DASCTH010000110.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000158121"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="QGH35_02315"
     CDS             complement(<1..246)
                     /locus_tag="QGH35_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81400.1"
                     /translation="MHFIPKKQIGLYDSAFEKESCGVGFIAHIKGQASRKIISDAAII
                     LENMDHRGARGAEHNTGDGAGILTGLPDAFLRRIAQKE"
     gene            538..2220
                     /locus_tag="QGH35_02320"
     CDS             538..2220
                     /locus_tag="QGH35_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HJL81401.1"
                     /translation="MNDLNFWLNAIGSILWHENVLYALLITGLILTVWSGFSQYRALT
                     HGVKVIRGDYDKPDDPGAITHFQALSTALSATVGLGNIGGVALAISLGGPGAIFWMWV
                     VGFLGMAVKITEVSLSMLHRDTSDPENPNGGPMWVMSNNLIGRSKIIRTLGMGIAGTF
                     SVALIINTFIAGNYFQAWNVGELTEQYFGIPSIVTGVILAVIVGAVIIGGIKRIGEVT
                     SKLVPFMLTLYVLSCLLVLAFNFNQIPSMFALIFNAAFSPLEASNAFIGGAVGSAFML
                     GMQRALFSNEAGQGSSPVAHSAAKTNEPIREGVVAGLEPFVDTIVVCTMSALVILISG
                     VWNRPAAIFINEAAFTPVSENEWQLSTDDSFYNQLSADQNVFVVVSGDLNSQSGNTLS
                     KITGEVIYDNEKKTVTNIKWQIFTTEKKPQLLDKGLYIDYVGAVLTAKAFDKAFERNW
                     PNLELGKWLVTLASWLFAISTMISWSYYGEKGVVFLMGQKSVLPYRIIFCLIIVFATL
                     GFIKTNEELNSLSVIGTGVCLVANLPIIWLFSHQAMREYKQYIRKLKAGDFL"
     gene            2249..3073
                     /locus_tag="QGH35_02325"
     CDS             2249..3073
                     /locus_tag="QGH35_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013756.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HJL81402.1"
                     /translation="MKKEIKIRPFWYSDVWIWPKLITIITTLDKKGVVNAGPYSHIMQ
                     YDVMTKNPRLIVGFRQDSHSFVNICDTKEFVVNCPNVDGLDDMMETAKFYPEGINELE
                     FTKFTQIPSRKVKPPSLKECPQIAECTVDEIVVLEKSTAIVIGTIEAIIVDEELISMN
                     REERIRAMDLPIGMGDEKRQDYYHTTLGKLTMHRLGQPKNAMAGADAVTNIPWDKEAM
                     KMLMNVPPPIRKMVIKNTEEFTKEKGATTVTSELFTELAKEVGMDPEVMERFNSGG"
     gene            3079..>3539
                     /locus_tag="QGH35_02330"
     CDS             3079..>3539
                     /locus_tag="QGH35_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJL81403.1"
                     /translation="MPIEINNHDLFKEQAYINGQWVDANDDSTVDVVNPVNQEVIGHV
                     PNCGQEETNTAINAAAEAQKKWKQYPAKEKASILRSLFDLICTHQEDLARIITYEQGK
                     SLTEATGEIAYSASFIEWFSEEAKRIYGDVIPGHLHDRRTVVIKQPIGVVA"
BASE COUNT         1072 a          631 c          748 g         1088 t
ORIGIN      
        1 ctccttttga gcaattctcc gtagaaaggc atccggcagt ccggttagga tgccagcacc
       61 atcccctgta ttatgttccg caccccttgc tcctcgatga tccatatttt caagaattat
      121 agctgcatca gatataatct tgcggcttgc ttggccttta atatgggcta taaaaccgac
      181 tccacagctt tctttttcaa aagcagaatc atataaacca atctgttttt ttggtataaa
      241 atgcataatt tttctcagca gctttgaggt tgctgataaa tgattactaa aaataatctt
      301 gatgttttgt tatagatact ctagacgcag aaagagatca gaaatcatca tttaaatcat
      361 tttatcataa aatcttattt atcatttgat taatagagga attatcattt agcaattatt
      421 gaaaaatttt tcttacctct tgtttttact atacagatct ttcatttata ctcattgggt
      481 ctcttgggtt attgttggat tcaacctaag aacatttatc ataaacaaag gggtgatatg
      541 aacgatctaa atttttggtt gaacgctatc ggtagtattt tatggcatga aaatgttctc
      601 tacgctttgt tgatcacagg gttgattctg acagtttgga gtggtttctc acaatatcgt
      661 gctttaactc atggcgttaa ggtaatccgt ggcgattacg acaaacctga cgacccaggt
      721 gctattacac acttccaagc tctttcaaca gcgttatccg ctacagtggg ccttggaaat
      781 attggcggtg tagcccttgc gatttcattg ggtggtcctg gagccatttt ctggatgtgg
      841 gttgtaggct tcttagggat ggctgtgaag ataacagagg tttcactttc aatgttacac
      901 agagacactt cagaccctga aaaccccaat ggtggcccaa tgtgggtaat gtctaacaac
      961 ctcatcggtc gatcaaaaat aataaggact cttggaatgg gtattgctgg tactttttct
     1021 gtcgctttga ttattaatac gtttattgca ggtaattatt ttcaagcatg gaatgttggg
     1081 gaattgaccg agcaatattt tggaattcct tctattgtga ccggtgtaat actcgccgtt
     1141 atagtaggag cagttatcat tggaggaatc aagcgtattg gtgaagtgac atcaaaactg
     1201 gtccccttca tgttaactct ttatgtgctc tcttgtttat tggttttggc gttcaatttt
     1261 aatcaaatcc cttccatgtt tgctttgatt tttaacgcag cattctctcc actggaggca
     1321 agcaatgcat tcattggagg ggcagttggt tctgctttta tgcttggcat gcaaagagct
     1381 ttgttttcaa acgaagcagg ccagggatct tctccagtcg cacattctgc tgccaaaacc
     1441 aatgaaccaa tccgagaagg agtggttgca ggtctagaac cttttgttga cacaatcgtt
     1501 gtttgcacaa tgagtgcttt ggtcatttta attagtggag tctggaatcg ccctgctgca
     1561 atttttatca atgaagcagc atttacccct gtttctgaaa atgaatggca actctcgacg
     1621 gatgattctt tttacaatca attatctgca gatcaaaatg tttttgtggt ggtgtctggt
     1681 gatctaaatt cacaatctgg aaacacctta agcaagataa ctggtgaagt catttatgac
     1741 aatgagaaaa agaccgtaac caacatcaaa tggcagatat tcacaacaga gaaaaaacct
     1801 caactattag ataagggatt gtatatagac tatgtcgggg cagtgctgac ggctaaagca
     1861 tttgacaaag cgtttgaaag aaactggcct aatttagagc tgggaaaatg gttggttact
     1921 ctagcttcct ggttgtttgc aatatccacg atgatttcat ggagttatta tggagaaaag
     1981 ggtgttgtgt tcttaatggg tcaaaaaagt gttctgccat acaggattat tttttgcctg
     2041 atcattgtgt tcgctacact tgggtttatt aaaactaatg aggaactcaa tagtctttca
     2101 gtaataggta caggcgtatg tcttgtagct aatcttccta taatctggtt gtttagccac
     2161 caggctatga gggaatataa acaatatatt cgtaagctta aagctggaga ttttttataa
     2221 ataagccttg aaaaatgaga ttaaaaccat gaaaaaagaa ataaaaattc gacctttttg
     2281 gtattcagat gtttggatct ggccaaagtt gattaccatt attacaactc tcgataagaa
     2341 aggtgttgtc aatgctggtc cttattctca cattatgcaa tacgatgtga tgactaaaaa
     2401 tccaagatta atagtggggt ttagacaaga ttctcattca tttgtaaata tttgtgacac
     2461 caaagagttt gttgttaatt gccccaatgt tgacggtcta gacgacatga tggaaaccgc
     2521 aaaattttac cccgagggaa tcaatgagct agagtttact aagtttactc aaatcccttc
     2581 acgaaaagta aaaccaccaa gtttgaaaga atgcccacaa attgcagaat gtactgtgga
     2641 tgagattgtt gtactagaaa aaagtactgc tattgtgatt ggcactattg aagcaatcat
     2701 tgtagatgaa gagttgattt caatgaacag agaagagcgc atacgcgcaa tggatttacc
     2761 cataggaatg ggtgatgaga aacgtcagga ttactaccat acaactttag ggaagctaac
     2821 aatgcacaga ttgggtcagc ctaaaaatgc tatggccgga gctgatgcgg tgaccaatat
     2881 accttgggac aaagaagcga tgaaaatgct tatgaatgtg ccgccaccga taagaaaaat
     2941 ggtcattaaa aatacagagg aattcaccaa agaaaaaggg gcaacaacag ttacctctga
     3001 gcttttcacc gagttagcta aagaagtggg tatggatcca gaagtgatgg aaagatttaa
     3061 cagcggaggt taaatttttt gcctattgaa ataaacaatc acgacttatt caaagaacaa
     3121 gcctacatta atggtcaatg ggttgatgcc aatgatgatt cgaccgtgga tgttgtcaat
     3181 ccagtaaacc aagaagttat cggtcatgtg cccaattgtg gccaagaaga aactaacact
     3241 gctatcaatg ctgctgccga agcacaaaaa aaatggaaac agtatcctgc aaaagaaaaa
     3301 gcctcaattc tgaggtcttt gtttgatctg atctgcactc accaggaaga tttagcaaga
     3361 atcataactt atgaacaggg caaatctttg accgaagcaa ccggtgaaat tgcatattca
     3421 gcctcattta ttgaatggtt ttcagaagag gctaaacgta tttatggaga tgtgattccc
     3481 ggccatcttc acgacagaag aactgttgtg atcaagcagc ctattggtgt tgtggcttg
//
LOCUS       DASCTH010000111         1527 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000158722, whole genome shotgun sequence.
ACCESSION   DASCTH010000111 DASCTH010000000
VERSION     DASCTH010000111.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000158722"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1527
                     /gene="feoB"
                     /locus_tag="QGH35_02335"
     CDS             <1..>1527
                     /gene="feoB"
                     /locus_tag="QGH35_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HJL81404.1"
                     /translation="IVVLTMWDVAKKIGTTIDLEKLSQLLKTRVIPIEGHTGEGLSEL
                     LEAIDQMISEPSVASSSNHFPEIDEAVEYFSETLDPENILSRTEIMRALIDQNGYAEQ
                     RLESKLNLVSNQQVEDIRHRFFDQESLFIKESRSRYQTINAIVKQVEQHNPIHRTWVE
                     KITNFIDQPVIGLVLFIAVLALVFQAVFSWATPMMEGIDILASKSSLWLKSTLEEGWF
                     RSLLTDGVIAGVGSVIIFLPQILILFCFIIILEDSGYLSRTAFIVEKIMRNFGLSGKS
                     VIPLISSFACAVPGIMATRVIPEPKARLITMLSAPFMTCSARLPVYALLIGAFIPDAY
                     YMNEWINLKGLVLLGLYVLGIFGGVLTALIMKTIMPAEPSPNFIIELFPYRTPNLKSM
                     FIKLYDRALIFLKRAGTVIFSVAILIWALAYFPRPIEIKNQFDIERDLVAVVADAKEK
                     SAQLVQINQEEKAAYLERSWLGQMGKSIAPVFKPLGWDWKVTAAFIGGLPAREVVIAA
                     L"
BASE COUNT          448 a          295 c          322 g          462 t
ORIGIN      
        1 gatcgttgta ctcaccatgt gggatgttgc aaaaaaaata ggaaccacta ttgatctaga
       61 aaaactctcg cagttattaa aaactagggt aatcccaata gagggtcata ccggtgaagg
      121 cttatcagag cttttggaag ccatcgacca aatgatttca gagccatcag ttgcaagttc
      181 gagtaatcat tttcctgaga ttgacgaggc agtcgaatat ttcagcgaaa ctctagaccc
      241 tgaaaatata ctctccagaa cagaaattat gcgagcattg attgatcaaa acggctatgc
      301 agagcaacga ctagagagta aattaaacct agtatcaaac caacaagtgg aagatattcg
      361 acatcgcttt tttgaccaag aatccttatt tataaaggaa tccagatcca gataccaaac
      421 gattaatgcc attgtcaaac aagttgaaca acacaatcct attcatcgca cttgggtaga
      481 aaaaataact aactttattg accaacctgt gatcggcctg gtgctattta ttgcggtgct
      541 ggctctggta tttcaggcag tattctcatg ggcaacccca atgatggaag gcattgacat
      601 actggcatcc aagtcgagtc tatggctaaa atctactctt gaagaaggct ggtttcgaag
      661 tttattgacc gatggagtta tagccggtgt tggtagtgtg ataatttttc tacctcaaat
      721 tctaattctt ttttgcttca tcatcatttt agaagactca gggtatttgt cgcgaaccgc
      781 tttcattgtg gaaaaaatta tgcggaattt tggactatct ggaaaatcag tcatccctct
      841 gatttccagt tttgcatgtg ctgttcctgg aatcatggca acaagggtga tcccggaacc
      901 taaagctaga ttaataacta tgctatccgc accctttatg acgtgctctg caagattacc
      961 agtgtatgcc cttttgattg gggcttttat acccgatgca tactatatga atgagtggat
     1021 taacttaaag ggattggtgc tactgggtct ttatgtcctg ggcatctttg gtggtgtttt
     1081 aacggccttg ataatgaaaa caatcatgcc tgcagaacct tcacctaatt ttattattga
     1141 gttatttccc taccgaactc caaatcttaa atcgatgttc attaagctgt atgatcgcgc
     1201 attaattttc cttaaacgtg ctggaacggt tatattttca gttgcaatcc tgatatgggc
     1261 tttagcttat tttccaagac ccatagaaat taaaaatcaa tttgatattg aacgtgattt
     1321 agtcgctgtg gtggcagatg ctaaagaaaa atctgctcag ttggtccaga ttaatcaaga
     1381 ggaaaaagct gcctatttgg aacgcagttg gttgggtcaa atgggcaaat ctatcgcacc
     1441 tgttttcaaa cccttgggct gggactggaa agttacagct gcatttattg gaggcttgcc
     1501 agcaagagaa gttgtgatcg cagctct
//
LOCUS       DASCTH010000112         2506 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000159513, whole genome shotgun sequence.
ACCESSION   DASCTH010000112 DASCTH010000000
VERSION     DASCTH010000112.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000159513"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="QGH35_02340"
     CDS             complement(<1..506)
                     /locus_tag="QGH35_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81405.1"
                     /translation="MLNNISSSSALKLGIAFSILFSGFIWLADWLWFNEPILLPDHTD
                     IGFWYAWQLKNPDIFSRSSAWILYLMHQGLIWLYIYKAQQSQPKYISGLHWFNISALL
                     TNALFIVLHLIQTHIWYDGLAQDVLEQSAQWSVIVLLFAVLMMENQRRGLFLGHSIDF
                     VTQASKTV"
     gene            631..1074
                     /locus_tag="QGH35_02345"
     CDS             631..1074
                     /locus_tag="QGH35_02345"
                     /inference="COORDINATES: protein motif:HMM:NF016955.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HJL81406.1"
                     /translation="MSRFFKFFDSSKSTDQIEKVVDPTQLATVVLMIEMARADFHQED
                     LEDQKIIDLLEHHFDLSHEDAVALFTRGQKKADDSVSLHEFTRTLHTNLSQADKEEVI
                     GLLWQLALVDDKLDRYEDYLVRKVADLLYVSNTVVLRIRHQVTAK"
     gene            complement(1061..2116)
                     /locus_tag="QGH35_02350"
     CDS             complement(1061..2116)
                     /locus_tag="QGH35_02350"
                     /inference="COORDINATES: protein motif:HMM:NF019375.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HJL81407.1"
                     /translation="MNDNPRGTALLYSGGLLTDLHAKTSHGLLRYSKRFEILGIIDHK
                     YAGRMSGEFVSNCQRDLPIFSDITKAIKELETKPEFLVIGIAFHGGQLPETHRNSVNQ
                     ALKAGINVVSGLHQVLGEDPEFAIRAKESGSIIYDIRKPRKVEDLSFWTGKVLSLKTP
                     RIAVLGTDCALGKRTTCQFLLQACDESNIKTELIYTGQTGFLQGFKHGFLFDATLNDF
                     VTGELEKAIMECDEISRPELMLIEGQSSLRNPSGPCGSELLLAGDVNGVILVHAIDRD
                     YFEGFEELKLKLPTIQEEIKLISNYGKKTIAVCINSDNKNLDTQALADQLHLPVVNPI
                     FESVSPIIEVIQKTILQ"
     gene            complement(2109..>2506)
                     /locus_tag="QGH35_02355"
     CDS             complement(2109..>2506)
                     /locus_tag="QGH35_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HJL81408.1"
                     /translation="KEMLNFPQELRDICVADESLHSPDDAYQLTKGMKPFGVFNIKLM
                     KCGGIYPALEIARIAENEGIELMWGCMDESKISITAALHAALASPATKYLDLDGFFDL
                     GWDLVSGGYICEHGVMQTNNKPGLGLHHE"
BASE COUNT          757 a          496 c          479 g          774 t
ORIGIN      
        1 cttacagtct ttgaagcttg cgtcacaaag tcaatcgaat gacctagaaa tagtcctctt
       61 ctttgattct ccatcatcaa gacggcaaac aaaagaacta taacggacca ctgggcagat
      121 tgttctagca catcttgagc aaggccgtca taccaaatgt gtgtttggat taaatgaaga
      181 acaataaata aagcattagt taataacgca gaaatattaa accagtgcaa acccgagata
      241 tatttaggct gactttgttg tgctttgtat atataaagcc agattaggcc ttgatgcatg
      301 aggtagagta tccaggcact agatctagag aatatatcag gattttttaa ttgccacgca
      361 taccaaaatc caatatcggt atgatctggc aacaagatag gctcattgaa ccatagccag
      421 tcagctaacc atatgaaccc tgagaatagg atactgaagg cgatacctag tttgagtgca
      481 gatgaggaag aaatattatt taacatacaa gaatctcatt cataatgctt ctgaattgta
      541 agataaacgc gatgcatatc aaacagttcg tttatagttt atgctttggt ttcaataatt
      601 ttgtttatgg aaagattata attaaatgtg atgtcacggt tttttaaatt ttttgactca
      661 tccaagagca ctgatcagat tgagaaggta gtcgatccga ctcaacttgc taccgtggtt
      721 ctcatgattg aaatggccag agctgatttt caccaagaag atcttgagga tcaaaagatc
      781 atagatcttt tagagcatca ttttgatctc agtcatgagg atgcagtggc cctatttacc
      841 cgaggtcaaa aaaaagccga tgattcggtt tctttgcatg aatttactcg cactttacac
      901 accaatctat ctcaggcaga taaggaagag gtcattggtc tattatggca actagctctg
      961 gtggatgaca aattggatcg ttacgaagac tacttggtaa gaaaagttgc agatcttttg
     1021 tatgtgtcaa acacggttgt attgagaata cgacatcagg ttactgcaaa atagtttttt
     1081 gtataacttc aatgattgga gaaacgcttt caaagattgg gttaacgacg ggcaaatgta
     1141 attgatctgc cagggcttga gtgtcaagat ttttgttatc ggagttaata caaacggcaa
     1201 tagttttttt cccatagtta gagatcagtt ttatttcttc ttgaatagtc ggtagcttta
     1261 acttgagttc ttcaaaacct tcgaagtaat ctcgatcaat agcatgaaca agaattacac
     1321 cattcacgtc ccctgcaagc aaaagttcag atccacaagg tcccgaaggg tttcttaagg
     1381 aggattggcc ttcgatcagc attaattctg gtcgactgat ttcatcacac tccataattg
     1441 ctttttctaa ttcacccgtg acaaagtcat tcagtgttgc atcaaaaaga aaaccatgtt
     1501 taaaaccctg caaaaacccc gtttgtcctg tatagataag ttctgttttt atgttagatt
     1561 catcacatgc ttgaagtaaa aactgacagg tagtcctttt gccaagggca cagtcggttc
     1621 ctaagacagc aattcttggt gttttcaagc tcaatacttt cccagtccag aaagataaat
     1681 cttctacctt tctaggtttg cgaatatcat agatgatgct tccagactct ttggcacgta
     1741 ttgcaaattc aggatcttca cctaaaactt ggtgtaagcc agaaaccaca tttatacctg
     1801 ctttcagggc ttgatttact gagtttctgt gagtttcagg tagttgacct ccatgaaaag
     1861 caataccgat gactagaaat tcaggctttg tttctaattc tttaatggct ttagtgatgt
     1921 cgctaaaaat tggtaggtca cgctggcagt tagaaacaaa ttccccagac atccggccag
     1981 catatttatg gtcaatgatg cccaggatct cgaagcgttt tgagtatcgc agcagtccat
     2041 gtgatgtttt tgcatggaga tcagtcaaca acccaccgct atagagtaaa gcagtgcctc
     2101 ttggattgtc attcatgatg aagtcccaaa cctggtttat tattcgtttg catcacccca
     2161 tgttcgcaaa tatagccacc agataccaaa tcccaaccta aatcaaaaaa cccatctaag
     2221 tctaaatatt ttgttgcagg actggccaat gccgcatgaa gcgctgctgt aatactaatt
     2281 ttactttcat ccatgcatcc ccacattaat tctatgcctt cattttctgc aattcttgct
     2341 atttctaatg ctggatagat accaccacac ttcatcagtt tgatgttgaa tacaccgaat
     2401 ggcttcatac cttttgttag ctggtacgca tcatctggag agtgcaggct ttcatcagct
     2461 acacaaatat ccctaagttc ttgtggaaaa tttagcattt ctttga
//
LOCUS       DASCTH010000113         2220 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000159838, whole genome shotgun sequence.
ACCESSION   DASCTH010000113 DASCTH010000000
VERSION     DASCTH010000113.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000159838"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(59..655)
                     /locus_tag="QGH35_02360"
     CDS             complement(59..655)
                     /locus_tag="QGH35_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020398137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HJL81409.1"
                     /translation="MITIREFFKDYKRIVILSIVVIILATIFSNIFIAIRNFFEAGGP
                     VLWGILFVTIIMWTLIVERFWFFNYVLPQRITDISHEWQLRKINRTWSSNMIRNELIS
                     QVSVETHRFILTIETMMQILPLLGLLGTVVGMIGVFDVMTFFGTGNARLMASGVSQAT
                     IPTMAGLVAALSGLYIANLLKRTADDQVHKLADLLIRK"
     gene            complement(683..2092)
                     /locus_tag="QGH35_02365"
     CDS             complement(683..2092)
                     /locus_tag="QGH35_02365"
                     /inference="COORDINATES: protein motif:HMM:NF013760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HJL81410.1"
                     /translation="MNSIINRLIFSLILLLIIGSQSYAQEELMGEEATDSVLSEKLKT
                     IETMEQLLDSVRVDGRQTGAVNDRREQKFLDARDQQQELLDQAMARLDREEQRSVRLQ
                     NEFETNEKILEDIGETLRIRLGNFGELFGVMRQVAGEVIATVRNSIVSLQYPNREHAI
                     IGLVESTGVPSIEQMSKLTVVMLEEMTQAGRVDRFQKQVVLPGGSTADAEIVRVGVFN
                     TITEDFFLKFIPDTTSLQVLARQPARRYRSMADDLFNLEKGYTTMAVDPSRGQILGLL
                     IQAPGLWERIRQGGLVGYFIIFIGLLGAVLSVWRLRILREEGRAINLQLATDKISTDN
                     PLGRILSVYDDNETIDPESLELKIDEAILREVPQLEKYHSIVKVFAAIAPLLGLLGTV
                     VGMIVTFQALTLFGTGDPKLMAGGISQALVTTMLGLIVAIPLVFLHSILTSWSGTLIE
                     ILEEQSAGLIARHAEKIKS"
     gene            complement(2089..2211)
                     /locus_tag="QGH35_02370"
     CDS             complement(2089..2211)
                     /locus_tag="QGH35_02370"
                     /inference="COORDINATES: protein motif:HMM:NF023358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HJL81411.1"
                     /translation="MWEELPRKYRNYVTQGLRVARKQVTPNLLELPIEAPGVVK"
BASE COUNT          646 a          499 c          370 g          705 t
ORIGIN      
        1 ggtgtgatat tgatttcact ttcatctttc tttcttttat attttctctt cattttgact
       61 attttctaat caaaagatct gccaatttat ggacttgatc atctgctgtt cttttcagaa
      121 gatttgcgat atataagcct gataatgcag caactaaacc tgccattgta ggaattgttg
      181 cctgagaaac accactagcc attaatctag cattgccggt tccgaaaaat gtcataacat
      241 caaaaacacc aatcatgcca acgacagttc ccaacaaacc tagaagaggc aagatttgca
      301 tcattgtttc tattgttaaa atgaatctgt gtgtctcgac tgagacttgc gagattaatt
      361 catttctgat catgttcgaa gaccaagtcc tattgatttt tctcaattgc cattcatgac
      421 taatgtcagt gattctttgg ggtagaacat agttaaagaa ccagaatctt tctacgatga
      481 gcgtccacat aataatggtg acgaacagaa taccccaaag cactggacca cctgcttcaa
      541 aaaaatttcg gatagcaata aagatattac tgaaaatggt tgcaagaatt atgactacaa
      601 tactgaggat tacgatcctt ttgtagtctt taaaaaactc tcgaattgta atcataaaat
      661 tgctcaaaac actcatcact atttatgatt ttattttttc agcatgtctg gcaatgaggc
      721 ctgcactttg ttcctctaga atttcaatta aagtaccgct ccagctggtc aaaatgctat
      781 gcaggaaaac caaaggtatt gcaacaatca atcctaacat tgtagtaacc agcgcttgtg
      841 aaattcctcc ggccatcaat tttggatcgc cggtaccaaa taacgtcagt gcctgaaagg
      901 ttacaatcat tccgacaaca gttccgagca aacctaacaa aggcgcaata gcagcaaaaa
      961 cttttacaat actgtgatat ttctccaatt ggggcacttc gcggagaata gcttcatcaa
     1021 tcttgagctc caacgattct ggatctattg tttcattgtc atcatagaca gaaagaatcc
     1081 ttcctaaagg attgtctgtg gagattttat cggtagctaa ttgcagattg atagcccgtc
     1141 cctcttcacg aagaatacgt aaacgccaca ccgacaaaac cgctccgagt agaccgataa
     1201 aaataataaa ataaccaact aatccacctt gtctgattct ttcccaaaga ccaggagcct
     1261 gaattaataa gcctagaatt tgccctctac taggatcgac agccatggtg gtatatcctt
     1321 tttctaaatt aaataaatca tccgccatag atcgatatcg tctagcaggc tgtctagcta
     1381 atacttgcaa gcttgttgtg tcagggataa atttaagaaa aaagtcctca gtaattgtgt
     1441 taaatactcc tactcgaaca atttctgcat cggcagttga tccacctggt aataccactt
     1501 gtttttgaaa tcgatccacc cttcccgctt gggtcatctc ctcaagcatg actacggtta
     1561 gcttgctcat ttgctcaatc gagggcactc ctgttgattc cactaatcca atgattgcat
     1621 gttctctgtt ggggtattgt aatgaaacta ttgaatttct taccgttgca attacttctc
     1681 cagccacttg cctcataaca ccaaataatt caccaaaatt accgagtctg attctcaatg
     1741 tttcaccaat atcttccaga atcttctcat ttgtttcaaa ttcattctga agcctaacac
     1801 ttctttgttc ctcccgatcc aatcgcgcca ttgcctgatc taagagttct tgttgttgat
     1861 ctcttgcatc caagaacttt tgttctcttc gatcgttaac ggctccggtt tgtcgaccat
     1921 ctacacgtac gctatcgagt aattgttcca tagtttcaat ggttttcagt ttctcgctta
     1981 atacagaatc agtggcctcc tcgcccatta gctcttcttg agcatatgat tgtgaaccta
     2041 tgattagcaa taaaatcaaa gagaaaataa gtctgtttat tatgctgttc atttcacgac
     2101 tcccggtgct tcaatcggta attccagcaa gttgggtgtc acttgctttc tagctactct
     2161 gagcccttga gtcacataat tacgatattt ccttggtaat tcttcccaca aatgagtttc
//
LOCUS       DASCTH010000114         1536 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000163698, whole genome shotgun sequence.
ACCESSION   DASCTH010000114 DASCTH010000000
VERSION     DASCTH010000114.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000163698"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="QGH35_02375"
     CDS             <1..913
                     /locus_tag="QGH35_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006534654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HJL81412.1"
                     /translation="TILEHRTIAKLKNTYTDKLPLLINKETQRVHTSYHQAVTSTGRL
                     SSSDPNLQNIPIRSAEGRQIRQAFIAPEGWKILAADYSQIELRIMAHISQDRGLLDAF
                     DQGLDIHQATAAEIFDTELEVISKEQRRSAKAINFGLMYGMSAFGLSRQLSIPRRNAE
                     QYIELYFARYPRVKDYMQSTVHQARKSGFVETVFGRRLYLPDIDTKHYQRRQYAERSA
                     INAPMQGTAADIIKKAMIDLQDQLENHAKEAKMVMQVHDELVLEVRENAVQKITALVS
                     QVMSQAANLEVALKVDVGVGDTWDEAH"
     gene            complement(920..>1536)
                     /gene="yihA"
                     /locus_tag="QGH35_02380"
     CDS             complement(920..>1536)
                     /gene="yihA"
                     /locus_tag="QGH35_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HJL81413.1"
                     /translation="FYINHTIRTVMLGFENIKFIKSANQVSQFPEDSGNEIAFVGRSN
                     AGKSTVLNVIFNRKNIAKTSKTPGRTQLVNFFKLDEDSCIVDLPGYGFANVSREKRNQ
                     WDRLLSSYFKTRAALTGVFLVVDGRRGITDLDRVFIDFYLPLEKSLHVLINKSDKLNK
                     SEQVLVLEQFKSYLGEVATIQLFSGTKKIGVEIAQNRLIEMLKK"
BASE COUNT          488 a          317 c          296 g          435 t
ORIGIN      
        1 aactattcta gaacaccgaa ccattgcaaa gctaaaaaac acctataccg ataagttacc
       61 gcttcttatc aacaaagaaa cacaaagagt ccacacctcc tatcatcaag cggttacctc
      121 aacaggtaga ctgtcttcct cggatccaaa tttacaaaat atacccatta gatcggcaga
      181 aggaaggcaa attagacaag cttttattgc tcccgagggg tggaaaatat tggcagcaga
      241 ttattctcaa atcgaactta gaattatggc ccatatttct caagatcggg ggttattgga
      301 cgcttttgat caaggactag atatccatca ggcaaccgct gctgagatct ttgatactga
      361 gctagaggtc atttcaaaag aacaaagacg atcagcgaaa gcaattaatt ttggtttgat
      421 gtatggaatg tcggcattcg gactttctcg tcagttgagc atcccacgcc ggaatgctga
      481 acaatatatt gaactatatt ttgctcgcta cccaagagtc aaagactata tgcagagcac
      541 agttcatcag gcgaggaaat ctggttttgt tgaaaccgtt tttggcagaa gactttattt
      601 accggatatc gatacaaaac actaccaacg cagacagtat gcggaacgaa gcgcaattaa
      661 cgcccccatg cagggaacag cagcggatat aataaaaaaa gcaatgattg atctacaaga
      721 tcaattagag aatcatgcca aagaagccaa aatggttatg caagtgcatg atgaactggt
      781 gttagaagtt cgagagaatg ccgttcaaaa aattactgct ctcgtttcac aagtgatgtc
      841 gcaagctgct aatttggaag tggctttgaa ggtggatgtt ggtgttggtg acacctggga
      901 cgaagcgcat tagaaggact tattttttta acatttcaat caacctattt tgtgcgattt
      961 ctacccctat tttcttggtt ccagagaaaa gctgtatggt agcaacctca cccaaataag
     1021 atttaaactg ctcaagtacc aggacttgtt ctgatttatt cagcttatct gatttattga
     1081 tcaatacatg cagcgatttt tccagaggca aataaaagtc aataaaaacc ctatctagat
     1141 cagtaatccc acgtctacca tccactacta aaaacacgcc tgtgagcgca gctctggttt
     1201 taaaatagct agatagtaac cggtcccatt ggttccgttt ttcccttgac acattcgcaa
     1261 agccataacc gggaagatcg acgatacaag aatcctcgtc cagtttaaaa aaattaacca
     1321 attgtgttcg acctggtgtt ttacttgttt tcgcaatatt ttttcgattg aagatgacat
     1381 tcaacactgt tgatttaccg gcattagatc gtcccacaaa tgcaatttca ttcccagaat
     1441 cttcaggaaa ttgcgacact tgattggcgc ttttaataaa ttttatattt tcgaatccca
     1501 acattactgt ccgtattgta tgattaatgt aaaatg
//
LOCUS       DASCTH010000115         1617 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000163760, whole genome shotgun sequence.
ACCESSION   DASCTH010000115 DASCTH010000000
VERSION     DASCTH010000115.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000163760"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="QGH35_02385"
     CDS             complement(<1..370)
                     /locus_tag="QGH35_02385"
                     /inference="COORDINATES: protein motif:HMM:NF023834.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HJL81414.1"
                     /translation="MSQYQAPIKDMLFVLDQLIGLDNITKLDGCEDINIDFVESVLTG
                     AAEIATEVIAPTNVDGDRYGVSLKNGIVEVPPGYQEAYDKYIQGGWSGLGFKPEFGGQ
                     GLPSTIAIPVNEIVMAANFSW"
     gene            611..>1617
                     /locus_tag="QGH35_02390"
     CDS             611..>1617
                     /locus_tag="QGH35_02390"
                     /inference="COORDINATES: protein motif:HMM:NF014126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HJL81415.1"
                     /translation="MSLCIPLIITPELTNQILNKAYDYVATNLGPTYMVMGISTLIFL
                     LILALSTYGNLVIGNEHEQPEYGLFGWSSMLFCSGIGASLVLWGTTEWVYYYLEPPFN
                     AEPESIEAIAWATSYGIFHWGITGWALYCLPAVAMAYAYHVRDYGTLRTSTACQSILG
                     NKASGVGGRIIDLIYMVALLGVLAGGLGFTTPTISANVTEFFGIEESLTVTISVLFIC
                     LLIFATSVHFGIERGIQKLSKFCVYLALFLLVYILIFGPTSFIVKTSLDSLIFTTKHF
                     IEMNIGPAPFGKSDFSKTWTVFYWAWFIGLGPFIGIFVARISRGRTLRTMIFGVLGFG
                     T"
BASE COUNT          479 a          294 c          306 g          538 t
ORIGIN      
        1 accaggaaaa attagcagcc attacgattt cattgactgg tatggcgatt gttgaaggca
       61 aaccctgacc accgaactca ggtttaaatc ccaaaccact ccaaccaccc tgaatatact
      121 tgtcataagc ttcttgatac ccaggcggga cttcaacgat gccattcttt aacgatacac
      181 cataacgatc tccatcaaca ttggtgggtg caatcacttc tgtagcaatc tcagcggctc
      241 cagttaaaac cgattcaacg aagtctatat tgatgtcttc acaaccatca agtttcgtaa
      301 tattatctag accaatcaat tgatcgagaa caaataacat atcctttatt ggtgcttggt
      361 actgactcat ataataacgc taagctcttt ttttaaacca taattagaaa ctcagtataa
      421 tacttagatt actgaataca ggacaaaaga aatacaaaat taaaattctc actttttttc
      481 agataagatt acaatttcat tacttttcat ctagattaaa tgtgttctaa aaaaggggag
      541 caatcaaatc attataaaag gcaaagaaaa tattgattgg tttatttttt ttactacagt
      601 cttaattata ttgagtcttt gtattccatt aattataact cctgaattaa ctaatcaaat
      661 ccttaacaag gcttacgatt acgtggctac caatttgggt ccgacctata tggtaatggg
      721 aattagcact ctcatattct tattaatact agctctaagc acatatggaa atttagttat
      781 tggtaatgaa cacgaacaac ccgaatatgg tctatttggt tggtcatcaa tgttattttg
      841 tagtggcatt ggagcttctc ttgttttatg gggaacgaca gaatgggtgt attactatct
      901 tgagccccct ttcaatgctg agcctgaatc gatcgaggct atagcctggg caacttcgta
      961 tggaattttc cattggggaa ttacgggctg ggctttgtat tgcctaccag cagtagccat
     1021 ggcttatgcg tatcatgttc gagattatgg gactttgaga accagcactg cttgccaatc
     1081 aatcctaggc aataaagcca gtggtgtggg tggaagaatt atagatttaa tctatatggt
     1141 agcacttttg ggggtattag ctggaggtct aggattcacc acgcccacaa tcagtgcaaa
     1201 cgttactgaa ttttttggaa tagaagaaag tttgactgtc acaatttccg tattatttat
     1261 ctgcttgtta atttttgcta ccagcgtcca ttttggcatc gaacgaggaa ttcagaaatt
     1321 aagcaaattc tgtgtctacc tggcattgtt tttgttggtt tatattttaa tttttggacc
     1381 gacaagtttc atcgtaaaaa cttctttaga cagcttaatt tttacgacga agcattttat
     1441 tgaaatgaac attggtcctg ccccttttgg taaatcagac ttcagtaaga cttggacggt
     1501 attttattgg gcctggttta ttggtttagg tccttttata ggtatttttg tggccagaat
     1561 atcgagaggt agaacattaa gaacgatgat tttcggtgtt cttggatttg gcacaat
//
LOCUS       DASCTH010000116         2851 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000164565, whole genome shotgun sequence.
ACCESSION   DASCTH010000116 DASCTH010000000
VERSION     DASCTH010000116.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000164565"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /gene="accC"
                     /locus_tag="QGH35_02395"
     CDS             <1..1281
                     /gene="accC"
                     /locus_tag="QGH35_02395"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HJL81416.1"
                     /translation="CSELNIKTVAIHSTSDSNQKHVLLADESVCIGPPESVNSYLNIP
                     SIISAAEVTDAMAIHPGYGFLSENSDFAEQVEKSGYVFIGPKPETIKLMGNKLNAIKV
                     AKKSGINTILGSHEALGDDSAAVLKMADKIGYPILVKAAAGGGGKGMRVAHTKANLQH
                     TIDLTRSEARAVFGDDTLYLERLLEEPRHIEFQMLADNYGNVVCLGDRDCSMQRNHQK
                     IIEEAPAPGIPLRQRTKMIQKCIKLCKQIGYRSAGTLEFLYENDEFYFIEMNTRLQVE
                     HPVTEMITGIDVVKEQIRIAMGFPLPFKQENITERGHAIECRINAEDARTFIPSPGKI
                     SLWHPPGGPGIRVDSHIYTGYEVPPFYDSMIAKIIAHANTRKSAIDRMRIALSEMVIE
                     GIKTNQNLHFEIMQHNAFKKGSTDIHYLEKRLGL"
     gene            1401..1718
                     /locus_tag="QGH35_02400"
     CDS             1401..1718
                     /locus_tag="QGH35_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJL81417.1"
                     /translation="MTKRAQKKPTSPISKNNSQSSQSLQTTQINGSLKNSTTIALEQY
                     FKMLNGHTPCNLYKLVIEEVELPLMKAVMDYTKQNQTQAAAILGINRSTLRKKLKAFD
                     LIQ"
     gene            1735..>2851
                     /gene="purH"
                     /locus_tag="QGH35_02405"
     CDS             1735..>2851
                     /gene="purH"
                     /locus_tag="QGH35_02405"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HJL81418.1"
                     /translation="MTANQPIKIQNALISVSDKTGLDELAKQLIAYQINLISTGGTAT
                     YLREHQCEVTDVSTITGFPEMMDGRVKTLHPLIYAGLLNRPNVDEDILEEYSITNIDL
                     VIVNLYPFERIVSTDSCNEAMAIENIDIGGPSMIRATAKNYHTKTILTDPDDYETLKT
                     ELTNNQGCVSFTLRKQLALKAFGYTASYDAAIWNYFSEKKNDTLPQTLPIGLTKKTNL
                     RYGENPHQRAAIYQPREAGLNTLVGSKLYQGKELSYNNLLDADAAMGCVQSFDEPACV
                     IVKHVNPCGVAMGNDLHEAYNKSFLADPESAFGGVIAVNRNVDSELIKNIINNQFLEV
                     LIAPSFDSDALKALERKQNIRVIEMDNCEKNTIDYSIQ"
BASE COUNT          992 a          536 c          549 g          774 t
ORIGIN      
        1 tgttctgagc tcaatattaa aaccgttgct attcattcca cttcagatag taatcaaaag
       61 catgtcctgc tcgcagatga atccgtctgt attggacctc cagaatcagt caatagttac
      121 ttgaatatac cgtctatcat tagtgcagca gaagtgaccg atgccatggc tattcatcca
      181 ggctacggtt ttttatctga aaatagtgat tttgctgagc aggttgaaaa aagtggttat
      241 gtttttattg gtcctaaacc tgaaaccata aaattgatgg ggaataaact aaatgccata
      301 aaagttgcta agaaatcagg cataaatacc atacttggtt ctcatgaagc gttgggagat
      361 gattcggcag cagtgctaaa aatggcagac aaaataggct atccgattct agttaaagca
      421 gccgctggtg gagggggtaa aggaatgcga gtagctcata ctaaagccaa tctgcaacat
      481 acaattgatc tcacaagatc agaagcccgt gcagtttttg gtgatgacac gctctatctg
      541 gaaaggttac ttgaagagcc aaggcacatt gaatttcaga tgcttgcaga taactatgga
      601 aatgtcgttt gtttaggaga cagggattgt tctatgcaac gaaaccatca aaaaataata
      661 gaagaggctc ccgcacctgg catccccctg agacaacgta cgaaaatgat tcaaaagtgt
      721 ataaaacttt gtaagcaaat tggatacaga agtgctggga cactggaatt tttatatgag
      781 aatgatgaat tctactttat tgagatgaat acaagattgc aagtagaaca tccggtgaca
      841 gaaatgatca caggtattga cgtggttaaa gaacagatac ggatcgcgat gggttttcca
      901 ttgccattta aacaagaaaa tataactgag agaggtcacg ccattgaatg cagaatcaat
      961 gcagaagatg ccagaacttt tataccatcc cctggaaaaa tttcattgtg gcacccacca
     1021 ggcggccctg gtattagagt ggatagccat atttatacgg gatacgaagt acctcctttt
     1081 tatgattcca tgattgcaaa aataattgcc catgcaaata ccagaaaatc ggcaattgac
     1141 cgcatgagaa tagccttatc tgaaatggtg atagagggta ttaaaacaaa ccaaaatcta
     1201 cattttgaaa tcatgcaaca taatgccttt aaaaaaggta gtacagatat tcattatttg
     1261 gaaaaacgac taggattgta aaattcttca caaacaattg cgatcttctg gtcactccat
     1321 gttatagttt ctttcactta aatttttaag ttacccagaa tctgaatctg gcaagaatat
     1381 gcaacagggg agttgtcttt gtgacaaaac gagctcaaaa aaaaccaaca tcaccaatat
     1441 caaaaaataa ttctcaatct agtcaatctt tacaaacaac acagattaat ggctcgctta
     1501 aaaactcaac aacaatagca ttagaacaat actttaaaat gctcaatggc cacactcctt
     1561 gtaatttata caaattagtt attgaagaag ttgaactgcc tttgatgaaa gcagtgatgg
     1621 actatactaa acaaaatcaa acacaagcag cagcaatact gggtattaat cgatcaacat
     1681 taagaaaaaa attaaaagca tttgatctca ttcagtgagc attttcttct ccatatgacg
     1741 gctaatcaac cgataaaaat acagaatgca ctgatcagtg tctcggataa aacaggcttg
     1801 gatgaacttg ccaaacaatt gattgcttat caaataaatt tgatctctac aggcgggaca
     1861 gctacttacc taagagaaca tcaatgtgaa gtaaccgatg ttagcactat aacaggcttc
     1921 ccagaaatga tggatggcag ggtcaaaaca ctacatcctc tcatctatgc tggtctcctc
     1981 aatagaccta acgttgatga agatattctg gaggaatact caatcacaaa catagattta
     2041 gtcattgtca atctttaccc atttgaaaga attgtttcaa cggatagttg taacgaagca
     2101 atggctattg agaacattga tattggtggc cccagtatga tcagagctac tgctaaaaat
     2161 tatcacacca aaaccatcct tactgatcca gatgactatg aaacactaaa gaccgaactt
     2221 accaacaacc agggttgtgt ttcattcact ctgagaaaac aattggcctt aaaagcattt
     2281 ggatacacag catcttacga tgctgccatt tggaattatt tttctgagaa aaaaaacgac
     2341 actctacctc aaacattacc tattggatta acgaaaaaaa caaacctgag atacggggag
     2401 aatccacatc aacgtgctgc aatatatcaa ccaagagaag ccggactcaa cacattggtt
     2461 ggatccaaac tgtatcaagg caaagaactg tcgtacaata acttattgga tgctgatgct
     2521 gcaatgggct gtgtacagag tttcgatgag ccagcctgtg tgattgtcaa acatgtcaat
     2581 ccttgtggag ttgcaatggg caatgatctt catgaggctt acaacaaatc atttcttgca
     2641 gatcccgaat cagcttttgg aggtgtcatc gcagtgaatc gaaatgttga tagtgaattg
     2701 ataaaaaaca taatcaacaa tcaattcttg gaggtcttaa ttgcgccttc atttgactct
     2761 gatgctctca aagctttgga acgaaaacaa aatattaggg tgattgagat ggataattgt
     2821 gagaaaaata ccattgatta ttctatccag a
//
LOCUS       DASCTH010000117         5802 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000164844, whole genome shotgun sequence.
ACCESSION   DASCTH010000117 DASCTH010000000
VERSION     DASCTH010000117.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000164844"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="QGH35_02410"
     CDS             complement(<1..229)
                     /locus_tag="QGH35_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81419.1"
                     /translation="MKQLDNNYGIITSVHNKTGMVELSNGSREHFLTKNNKIKPVVGD
                     YVLYDPLDEETFLITEILESNNQLMRLNTKRQ"
     gene            281..829
                     /gene="orn"
                     /locus_tag="QGH35_02415"
     CDS             281..829
                     /gene="orn"
                     /locus_tag="QGH35_02415"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HJL81420.1"
                     /translation="MIKQSQRLIWIDLEMTGLDTQTDSIIEIATVVTEGDLEVIAEGP
                     NLAIHQSETALEQMDNWNQNQHSKSGLLERVRRSNINTQEAEELTLEFLQKITEKGQS
                     PMCGNSICQDRRFMARLMPNLEAHFSYRNLDVTSLKIISHLWAPEVARSHSKSSKHLA
                     REDIFDSIYELKHYKNHLLNLD"
     gene            complement(834..1211)
                     /locus_tag="QGH35_02420"
     CDS             complement(834..1211)
                     /locus_tag="QGH35_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HJL81421.1"
                     /translation="MTFAYVCIFIAMLLPMYWAGMAKKESKIPFDNNAPRDHIAQLKG
                     KAKSAYCIEQNSYESFPPFAIAVVVAHLTGGVQLTVDILAGIYVASRIIFGLCYLQGK
                     GTMRSITFMVGLVCIVALFFVGG"
     gene            1334..1849
                     /locus_tag="QGH35_02425"
     CDS             1334..1849
                     /locus_tag="QGH35_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81422.1"
                     /translation="MEKRLKLFATEKIKEVKPDLLIDDLYSVYEQNYNILYLLLSKPL
                     AQYTGSYFSTKTTIELILTSNLGQREIQINMNILSVYTVDIEMHYQKNDSEKISYLFN
                     LRARLFLDAKILEVKRVDLNPNSLHRNENSTMLEFDDSKPEHGVQSNLTKSLIVNKWF
                     KKLINDRYKLK"
     gene            1854..3236
                     /locus_tag="QGH35_02430"
     CDS             1854..3236
                     /locus_tag="QGH35_02430"
                     /inference="COORDINATES: protein motif:HMM:NF017760.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81423.1"
                     /translation="MEKPNPLSNPVFRNLFIAQVIALVGTGLSTVGLSLLAYNLAGDN
                     AGRVLGIALAYKMIAYVFFAPLAGAIVHRFDRKPFLISLDIIRALLVLLMPFVSQLWH
                     IYALIFLLNLFSAGFKPVFQAVIPDILEDEEQYGKALSYSRLAYDLENLLSPSFAAIA
                     LFFFSYSSLFVGNSMAFICSAAIIVVTMMPIAKPAQRTGSLIDEVSFGIKSYFKTPRL
                     RTLLILYFAVACSSAMLIVNTIIYVKEYLGGSDSDLAIFFAFSGFGSMLAALSYPKIS
                     KHIQDKIIIQSGTLLMSIGLVLMSTMPSYFYALPCWFLVGIGWSLVQTPSGKIVNISA
                     SPSDRTSYFSAQFALSHLCWLITYPITGFLVLYYGFSFSALFLSCIVLMCFIFSILFW
                     PNEQDSNLTHSHESKFHTHDHNHKDQHHQHSHDDDNLEETHGHQHEHQNISHKHRFVI
                     DLHHQEWPKG"
     gene            complement(3215..5275)
                     /locus_tag="QGH35_02435"
     CDS             complement(3215..5275)
                     /locus_tag="QGH35_02435"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HJL81424.1"
                     /translation="MIGVLIISFPIQHLQSTDETIPEVLISKPEDSHLLQLVTALTEQ
                     AHYSKKNVTNELSPFILVNFLEELDPSKMYFTKSDFHYFQRYRYQIDDALKQGKLEPI
                     FDIFRIYRLRVQQRLEFCMNFILKKKDFNTDDYYDFDRNIKDWSNNEIGLKSLWQGKV
                     QNDLLMIILAGQTQTEAVQILNKRYSRFVKTVNSYKKHDVINIFLNAYVQTLDPHSIY
                     FDPLQAEEYEIQMSLSYQGIGARLNMENELVNVVDVIPGSPASKDGRLKSGDKITGIL
                     DQKNKSLIDVIGWNLNEVVKLIRGPKGSEVTLQIMPLDSNLESPYLLTLKRDEVQLED
                     QAASSVIEELQMNDRTYSIGIITIPSFYQDFRAKKRNEETFRSTSEDVKEIVDNLMEI
                     GIDALLIDLRGNGGGLLTEATALTGLFIDDGPVVQLKDSRNKIEIIDDPISGSIYKGP
                     LAVMVDRFSASASEIFAGAIQDYSRGIVIGQQTFGKGTVQSIYPLDRFSRFKSKNGFG
                     QLTLTIGKFYRISGSGTQNRGVVPDIELPSFINKDRFGEETKKNTLPWDRITSLKYDH
                     DLSLDKSIALLQDKYSQRSKNNLPLKFLKEDIDSIKIQNQNTRLSLNLDIRINEREQI
                     KIANQERRTKRLEQLGYADNEDFDDFVSKTLLNEVLKMMVDLIDYNDPKPDDSQPNQP
                     FGHS"
     gene            complement(5326..>5802)
                     /locus_tag="QGH35_02440"
     CDS             complement(5326..>5802)
                     /locus_tag="QGH35_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81425.1"
                     /translation="INKSLSKKQNSFNVLPTEDFQNMLYPWLEPRTAPQTPEDLPQLL
                     SRVLVRETMETHNVKYLINISGETKTNSSSGALSCAAGPGGGGCFGLAWWDDTSSYSA
                     SVWDIAQESSVGSVSATVSGTSVIPALIIPIPIIARTKSSACNGLVTQLLKFFSTS"
BASE COUNT         1753 a         1049 c         1013 g         1987 t
ORIGIN      
        1 cctgcctttt agtatttaat cgcatcaatt gattgtttga ttctaagatt tcggtaatca
       61 aaaacgtttc ctcatccagc gggtcgtaga gcacataatc tccaacaact ggttttattt
      121 tattattttt ggtaagaaaa tgttcccgag aaccattgga taattcgacc atacccgttt
      181 tgttgtgaac ggatgtaatg attccataat tattgtccaa ctgtttcata gataatcttt
      241 agtgattata caatcattac tttgttaata tgttcttgtt atgattaaac aatcacaaag
      301 actgatctgg attgatcttg aaatgactgg cttggatact caaacagatt ccattattga
      361 gatcgccact gttgttactg aaggggatct tgaagtcata gcagagggtc caaatttagc
      421 tatccatcaa agtgaaactg ctttagaaca aatggataac tggaatcaaa accaacattc
      481 taaatctggg ttattagaaa gagtaagacg cagcaacatc aatacccaag aggcagaaga
      541 actgactttg gaattcttac aaaaaataac tgagaaaggg cagtccccaa tgtgtggcaa
      601 cagtatctgt caagatcgga gattcatggc tcggcttatg ccaaatctgg aagctcattt
      661 tagttataga aatttagacg ttacttcctt aaaaatcatt tctcatttat gggcaccgga
      721 agttgcccga tcccatagca aatcatccaa acatctcgcc agagaggata ttttcgactc
      781 catttatgaa ctaaaacatt ataagaatca tttgttgaac ctagactgat cgactaaccg
      841 ccaacaaaaa acaaagcaac aatacaaact aaaccaacca tgaatgtgat tgacctcatt
      901 gttcctttgc cttgtaagta acacaatcca aatattattc ttgatgctac atatatgccg
      961 gccagaatat caacggtcag ttgtactcct cctgttaaat gtgcaacaac tacagcaatt
     1021 gcgaaagggg ggaaagattc atagctgttt tgctcaatac aataggcgct ttttgctttt
     1081 cctttgagtt gagcaatgtg gtctctcggt gcattattat caaatggaat cttggattct
     1141 tttttagcca tacccgccca atacataggt agcaacatag ctatgaaaat acaaacgtag
     1201 gcaaatgtca ttgtgattct cctatttcag tagattttta aattttagtt tttaaattca
     1261 tagtttaatt gatttataat tccatttaac agaaaagtag cattagtcct gaaactttgt
     1321 gagagcgggg aaagtggaaa agcgtttaaa attatttgca acagaaaaga tcaaagaggt
     1381 taaaccagat ctgttaattg atgatctgta ttcagtctac gagcaaaact acaacattct
     1441 gtatttgctg ctatcaaaac cattagcaca atacacagga tcgtatttca gcacaaaaac
     1501 aactattgag ttgatactga cctcaaattt aggacaacga gaaatccaga taaacatgaa
     1561 tatattatct gtctacacgg tggatataga gatgcattat caaaagaatg attcagagaa
     1621 aattagttat ctctttaact taagagccag gcttttttta gacgcaaaaa ttctggaagt
     1681 gaagcgtgtt gacttaaatc caaacagtct acataggaat gagaattcca ctatgttgga
     1741 atttgatgac tctaaacctg agcatggtgt tcagagtaat ttaacaaaga gcttgattgt
     1801 caataaatgg tttaaaaaat taatcaatga cagatacaag ttgaaataaa aagatggaaa
     1861 aacctaatcc acttagcaat cctgtcttta gaaacctgtt cattgcacaa gtgattgcac
     1921 tggttggaac tggactttca accgttggtt tatcattatt agcctacaat ttagctggag
     1981 acaatgcagg tcgagttcta ggtattgcgc tagcctataa aatgatcgct tatgtttttt
     2041 ttgcaccgct tgctggtgcc atcgttcatc gttttgatag aaagcccttt ttaatttctt
     2101 tggatatcat cagagcatta ctggtgctat tgatgccttt tgtttcacag ttgtggcata
     2161 tctacgccct aattttttta ttaaacttat tttctgcagg atttaaacct gtttttcaag
     2221 ccgtcatacc cgatattctt gaagatgaag aacagtatgg aaaagcactt tcatattcaa
     2281 ggcttgctta tgatttagaa aacttgctca gcccatcttt tgctgcaatc gctttattct
     2341 tcttctcata ttctagttta tttgtgggta actcaatggc ttttatttgt tcagcagcca
     2401 ttattgtagt cacaatgatg cctattgcta aacccgctca gagaaccgga tcactcatag
     2461 atgaggtcag ttttggtatt aaatcctatt ttaaaacacc tcgtttaagg acactcttaa
     2521 tcctttattt tgcagtagct tgttcgagtg caatgttgat tgtaaatacg atcatctatg
     2581 tcaaggaata cttaggtggc agcgatagtg atttggcaat attttttgct ttctctggtt
     2641 ttggatcgat gttagcagcg ttgtcctatc caaaaatatc aaaacacatc caagataaaa
     2701 taattatcca atcaggaact ctattgatgt cgattggctt ggttttaatg tctacaatgc
     2761 catcttattt ttatgcttta ccatgttggt tcttagttgg aatagggtgg tcgcttgtgc
     2821 aaacgccatc tggtaaaatc gtcaacatct ctgcgtcccc atcagataga acatcttatt
     2881 tttctgctca atttgcactg tctcacttgt gttggctgat tacttacccc atcactggtt
     2941 tcctggtttt gtattatgga tttagtttta gtgcactatt tttaagttgt atcgttttga
     3001 tgtgctttat ttttagtatt ctattctggc ctaacgagca agatagtaat ttaacacata
     3061 gtcatgaatc aaaattccat acgcatgatc ataatcacaa ggatcaacat catcaacatt
     3121 cacatgatga cgataatctg gaagaaaccc atggtcacca acatgagcac caaaacattt
     3181 ctcataagca tagatttgtc attgatttac accatcagga atggccgaaa ggttgatttg
     3241 gctgactatc atctggcttt ggatcattat aatctattag atccaccatc atctttaaaa
     3301 cctcgttgag taatgttttt gaaacaaaat catcaaagtc ttcattgtct gcataaccaa
     3361 gttgttccag tctttttgta cgtctttctt ggttagctat ttttatctgt tctctttcgt
     3421 ttatacgaat atcaaggttt aaggatagtc tggtattttg attttgaatt ttaatactat
     3481 caatatcttc tttaaggaat ttaagtggta agttattttt agatctttgt gaatatttat
     3541 cttgtaaaag ggcaatagat ttatctaagg ataaatcatg atcatacttt agagatgtta
     3601 tccgatccca gggaagcgta tttttcttag tttcttctcc aaatcgatct ttattaataa
     3661 aggacggtaa ttctatatct ggaacaacgc ctcgattttg agtgccgctc ccagatattc
     3721 tataaaattt tccaatcgtt aaggtcaatt gaccaaaacc attttttgac ttaaatctag
     3781 aaaaacgatc aagagggtag atactttgta ctgtaccttt accaaaggtt tgttgtccta
     3841 tcacaatgcc tctagaataa tcttgaattg ctcctgcaaa tatttcagaa gccgatgcac
     3901 tgaagcgatc caccataaca gctaaaggtc ctttatatat ggaacctgaa atcggatcat
     3961 caatgatttc aattttattt cttgaatctt tgagctgaac aacaggtcca tcatcaataa
     4021 acagtccagt taatgctgtt gcttcagtca gtaatccacc tccgttacct ctcaggtcta
     4081 tcagcaaggc atcaatacca atctccatta ggttatcaac aatttcttta acatcttctg
     4141 aagtgcttct aaacgtttct tcgtttcttt tttttgccct aaagtcttga tagaagcttg
     4201 gaattgtaat tatgccaatg ctatatgttc tatcgttcat ctgaagttct tcaataacac
     4261 ttgaggctgc ttggtcttct aattgaactt cgtctctttt aagcgtcaat aggtagggtg
     4321 attctaagtt ggaatctaaa ggcatgattt gaagtgttac ctcgcttcct ttaggtcctc
     4381 gaattagttt aacaacttcg ttcaaattcc agccaataac atctatgagt gatttatttt
     4441 tttgatctag aattccagta attttatccc ctgatttcaa acgaccatct ttagaagctg
     4501 gacttcctgg aattacatca acaacattaa ctagctcgtt ttccatgttt agtcgtgcac
     4561 caatgccttg gtaggatagg ctcatttgta tttcatattc ttcagcttgt aaggggtcaa
     4621 agtaaattga atgtggatct agagtttgaa cataggcatt cagaaaaata ttaatgacat
     4681 cgtgtttttt ataagagttg accgtcttaa caaatctact gtatcttttg ttcagaattt
     4741 gtacagcttc tgtttgtgtt tgacctgcaa gaataatcat caataggtca ttttgtactt
     4801 ttccttgcca tagtgatttt agtccgatct cattatttga ccaatctttt atattacgat
     4861 caaagtcata ataatcatct gtattaaagt cttttttctt caaaatgaaa ttcatacaaa
     4921 attccaatcg ttgttggaca cgcagcctgt agattctaaa aatatcaaaa atcggttcca
     4981 acttcccttg tttaagtgcg tcatcaattt gatatcgata tcgttgaaaa taatgaaagt
     5041 ctgattttgt aaaatacatc tttgaagggt caagttcttc tagaaaatta actagaataa
     5101 agggtgaaag ttcattggta acattctttt ttgagtaatg agcttgctca gttaaagctg
     5161 taaccaattg caacaaatgg ctgtcttcag gttttgaaat cagaacttct ggaatagttt
     5221 catctgttga ttgaaggtgc tgaataggga acgaaatgat gagtactccg atcaaaaggt
     5281 agattatttt tttttgacta aaaatgattg attcgttaca aacgtttaac tagttgaaaa
     5341 gaattttaac aattgtgtta ccagtccatt acacgctgaa gattttgttc ttgctatgat
     5401 cggaatcgga atgatcaatg caggaataac cgatgttcca ctgacggttg cactgacact
     5461 tcctacagat gattcttgtg ctatgtccca cactgatgct gagtatgagg atgtatcatc
     5521 ccaccaagct aatccaaaac agccaccacc acctggacca gctgcacatg atagggctcc
     5581 agaacttgag ttggttttag tttcaccgct gatattaatt aaatatttaa cattatgtgt
     5641 ttccattgtt tctcgcacta aaactctact caataattga ggtaagtctt ctggtgtttg
     5701 tggggcagtt cgcggttcaa gccatgggta aagcatattt tggaaatctt ctgttgggag
     5761 aacgttaaag ctattttgtt tttttgaaag agatttatta at
//
LOCUS       DASCTH010000118         6925 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000165159, whole genome shotgun sequence.
ACCESSION   DASCTH010000118 DASCTH010000000
VERSION     DASCTH010000118.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000165159"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="QGH35_02445"
     CDS             <1..500
                     /locus_tag="QGH35_02445"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJL81426.1"
                     /translation="YMVPSGGFILNNEMDDFSAKPFTPNRYGLVHGEANSIHPNKRML
                     SSMTPTFIESDAGTAILGTPGGSKIITMVLLSILNWTNGGTANEMNSIKRYHHQYLPD
                     TIQYEKGAFSERVVDELTDMGHKLKEIRDYGNMQVITIQKDNGKVETSSDPRALIEDS
                     GIDYY"
     gene            complement(501..1322)
                     /gene="mutM"
                     /locus_tag="QGH35_02450"
     CDS             complement(501..1322)
                     /gene="mutM"
                     /locus_tag="QGH35_02450"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HJL81427.1"
                     /translation="MPELPEVEVTKKSLEDCLKGRSIKSIDIREKRLRWNLNQTDLNN
                     LIGQKIQTLTRRGKYILINTEPGSALLHLGMSGSIGILEIDQPLKKHDHFDLIIDSNH
                     IIRFNDPRRFGSFIWAGKKPEKHKLLSKLGPEPLENKSLGSYLHSLSKNRKTSIKAFI
                     MNAQVVVGVGNIYANEALFISGIHPKRKASRVSKKRYMQLASAIQETLTKAIAMGGTT
                     LRDFSYSYRGKKIGYFKQELFTYGREGSQCKNCSRPIKQITLSGRSSFYCSGCQH"
     gene            complement(1361..1516)
                     /gene="rpmG"
                     /locus_tag="QGH35_02455"
     CDS             complement(1361..1516)
                     /gene="rpmG"
                     /locus_tag="QGH35_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014714411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HJL81428.1"
                     /translation="MREKIKLVSSAKTGHYYTTTKNKKEHPDKMETKKFDPTIRKYVL
                     YKESKIK"
     gene            complement(1517..1753)
                     /gene="rpmB"
                     /locus_tag="QGH35_02460"
     CDS             complement(1517..1753)
                     /gene="rpmB"
                     /locus_tag="QGH35_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010630182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HJL81429.1"
                     /translation="MSRICQVTGKKTISGNNVSHANNRNRRKFLPNLHMHRIWVESQN
                     RFVKLKLSKKGLRTIDKLGIDHVLENINKYGETE"
     gene            1940..2398
                     /gene="dut"
                     /locus_tag="QGH35_02465"
     CDS             1940..2398
                     /gene="dut"
                     /locus_tag="QGH35_02465"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HJL81430.1"
                     /translation="MKKKVDIKVLNQRISKDFGVPQYETEGSAGIDLRACIDSPINLT
                     PGQTELIPSGIAIHINDPTLAAVILPRSGLGHKQGLVLGNLVGLIDSDYQGQVFISCW
                     NRGNQLILIEPGLRLAQMVFVPIKQVDFRVVEEFDMTARGEGGFGHTGKD"
     gene            complement(2395..3048)
                     /gene="pyrE"
                     /locus_tag="QGH35_02470"
     CDS             complement(2395..3048)
                     /gene="pyrE"
                     /locus_tag="QGH35_02470"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005273475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HJL81431.1"
                     /translation="MENNKQEFIRFALTKGVLKFGEFKLKSGRISPYFFDLGLFNSGQ
                     SIDCLGRFYAAALMASTLEYDMLFGPAYKGISITTVLSSALARDYKIDIPFAFNRKET
                     KDHGEKGLIIGAPIEGRVLIVDDVITAGTAVSECARLITDLGGTLAGILISLDRQERG
                     RENLSAVAEVSVKLSVPVIAIANLNDLIDLLESENTAIKQWLPKIKKYRDRYGVGVD"
     gene            3158..3931
                     /locus_tag="QGH35_02475"
     CDS             3158..3931
                     /locus_tag="QGH35_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HJL81432.1"
                     /translation="MRIITVNVNGIRAAARKGFFEWLPKQGADIVCLQEIRAQLDDIQ
                     SETFWPEQYHCFYFPAEKKGYSGVAIFSKKKPSSILEGLSSQEFDSEGRYLECVYDDF
                     TIASVYFPSGSSGDIRQQMKYRFLDVFEDKLKDKLQSRKPYIFCGDVNIVHKEIDIKN
                     WKSNQKNSGCLPEERAWLDKIFDQLCFVDSFRVVNQNADQYTWWSNRGRAWEKNVGWR
                     IDYQITTQNFKNCVNKSFIYRKKRFSDHAPLVIDYDYPF"
     gene            3934..5196
                     /locus_tag="QGH35_02480"
     CDS             3934..5196
                     /locus_tag="QGH35_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018015272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81433.1"
                     /translation="MINKQQHGLGIYLKPEVIRMVFLGFSAGLPYPLLFATLTVWLAS
                     VDIGIAEVSMFGWVGVIYAMKFLWAPMLDSLSIPFLSKTLGQRRAWMLLMQCCILIGL
                     FYMSFFKPQTNLSWLAYLAIIIAFASATQDIAIDAYRIEIISNRFQGAMAASYQLGYR
                     ISVLLSGAGALYIAEFYSWKLAYQTMALFMLVGVATVLIISEPKEQKSSQYSIHWFKE
                     SVIHPFSEFFTRNGYWSIMLLLFIALYRVSDLMLNIVANPFYIDIGFKLSEIASITKV
                     FGFIVTIIGAFVGGLSVAKYGISKPLIVSSILLAGTNLFFLLLESLGADLRILILTIS
                     ADNFALGFSGSVFIAFLSSLTNRNYTASQYALFSSLMFLPGITLSGFSGQFIESVGWS
                     DFFLFAAAMGLPAIVFAVIIVKKGWQEQ"
     gene            complement(5202..6059)
                     /locus_tag="QGH35_02485"
     CDS             complement(5202..6059)
                     /locus_tag="QGH35_02485"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81434.1"
                     /translation="MILITGATGNTGSAAVQELSSRNIPFRALIRNKDKQSQITDLGG
                     EVIIGAIEDDGALNQAMKGVQKTLVLLPNSEQQLFLEKKIVDTALAANVQHIVKISSM
                     EAVPEATSPIPKLHMQSENYIKHSGMSWTMIKPNFFMQNLLGSGKTIVEQDKFFLPMG
                     EGKTGMIHTRDIGMVIAKVLSEDGHAGQNYEVTGPEILSFHDVAEIFSRTLGKKVDYI
                     NVPIDEYKKTLSQFLTNQWHLDSVIDLFKGIAAGGIEDKTNTFQDLMGKSPCSLEQFI
                     QEYSFVFNA"
     gene            complement(6061..>6925)
                     /locus_tag="QGH35_02490"
     CDS             complement(6061..>6925)
                     /locus_tag="QGH35_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013044.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HJL81435.1"
                     /translation="YPILLIKDKNNKINGFHNVCRHRASKVLQQNRGNCQSMICPYHG
                     WNYGLDGNLKNPPNFFGGKDFNYKKHSLFPIRVSEKYGLIFVNFSLKSQSLNDWLGPF
                     NKVINDYYDNDYIFHNELKFDVHANWKTYVDNYQEGYHIPLVHPQLNRDVVWEDYTIE
                     NTELCSIHSVPARLGSNQPGSFGWHFPNFIFNVYGRGIVFQRIEPIQAKWCRVVYNLF
                     RPSSVSFDDFESSEGKYQLDVSLEDQQLVPEIQRNLQAGIYQSGPLSSKYESGVAHFH
                     QLLMKKITKGE"
BASE COUNT         2080 a         1229 c         1234 g         2382 t
ORIGIN      
        1 gatatatggt tccatcaggt ggctttatat taaacaatga gatggatgat ttttctgcta
       61 agccgtttac tccaaatcgc tatggcttgg ttcatggcga agcaaattca attcacccaa
      121 acaaacgaat gctctccagt atgacaccta catttatcga atctgatgct ggaactgcca
      181 tcttaggcac gccaggaggc tcaaagatta taaccatggt tcttttgtcc attctcaatt
      241 ggactaatgg tgggacagcc aatgaaatga actcgatcaa acgatatcat catcagtatt
      301 tgccggacac aattcaatat gaaaaaggcg ctttttccga acgtgttgtt gatgaattga
      361 ctgatatggg ccacaagcta aaagaaattc gtgattatgg aaatatgcaa gtgattacca
      421 tacaaaaaga taatggcaag gtagaaacct catctgaccc gagagctttg atagaagata
      481 gtggtattga ttactattaa ttaatgttga caaccagaac agtaaaaaga gcttctacca
      541 ctaagtgtta tctgcttaat tggcctagaa caattcttac attgagatcc ttcacgacca
      601 taagtaaata actcttgttt aaaataaccg atcttctttc ctctatatga ataagaaaaa
      661 tctcttagtg tagtcccacc cattgctata gcttttgtta gggtctcttg aatcgcagat
      721 gctaattgca tatatctttt tttgctgact ctgcttgctt tgcgtttagg atgaatacca
      781 ctaataaata atgcttcatt tgcataaata ttgccaacac caacaactac ttgtgcattc
      841 attatgaagg cttttattga ggtttttcga ttcttgctta agctgtgcaa ataggatccc
      901 aaagacttat tttccaaagg ttcaggtcca agtttactta atagtttatg tttttcaggc
      961 ttctttcctg cccatataaa actaccaaat cttcttgggt cgttaaaccg tataatgtgg
     1021 ttgctatcta taatgagatc aaaatggtca tgttttttta aaggctgatc aatctccagt
     1081 atgccaatac ttcctgacat gcctaagtgt agtagtgcac taccaggttc ggtattgatt
     1141 aagatatatt tccctcgtct tgtaagagtt tgaatttttt gtcctataag gttatttaaa
     1201 tcagtttgat ttaaattcca tcgaagtctt ttttctctaa tatcaatgga ttttatagat
     1261 cgccctttaa ggcaatcttc tagggatttt tttgttactt caacctcagg taattcaggc
     1321 atattttata aattatgcct acatgtttta tggaatgaaa ttatttaatt tttgactctt
     1381 tgtaaagaac gtattttcta attgtagggt cgaacttctt tgtctccatt ttatctggat
     1441 gttctttttt gttctttgtt gtagtgtagt aatgcccagt ttttgctgag gatactagct
     1501 ttattttctc acgcatttat tcagtttctc catatttatt aatgttttcc aaaacgtgat
     1561 ctattcccag tttatcaatg gtcctcagac cttttttcga tagttttaac ttaacaaatc
     1621 tgttttgact ctcaacccag atccgatgca tatgtaaatt gggcaaaaat ttacgtcgat
     1681 ttctgttatt agcatgagaa acattgttcc cacttatcgt tttttttcca gtgacttgac
     1741 aaattctaga cattttttgc tcttttttct tgctagttat ctgatcaatt atagcaaatt
     1801 gttttagtga tcgagtttta taccaagaca aatccaacat agcaagctct gagcataaaa
     1861 atattcataa gagcccttat aagatgtagt atttagttct aacctattgt agtttctcat
     1921 tcaaatagag aattaatgta tgaaaaagaa agtagatata aaggttctta atcagagaat
     1981 tagtaaagat tttggagtgc ctcagtatga gaccgaagga tctgcaggca tagatttaag
     2041 agcttgtata gactcaccga ttaacttaac tccagggcaa actgaattga tacccagtgg
     2101 tattgcgatc catattaatg atccaacttt agctgcagta atattgccaa ggtccgggtt
     2161 aggccataaa caaggcttag ttttaggtaa cttagtcggt ctaattgatt cggactacca
     2221 aggccaagta tttatttcat gttggaacag agggaatcaa ttgattctaa ttgagcccgg
     2281 cctcaggtta gctcagatgg tatttgtacc gataaaacaa gttgatttta gagtggtaga
     2341 agaatttgat atgaccgctc gtggcgaagg aggctttggt catactggca aagattaatc
     2401 aacacctaca ccatagcgat ccctgtattt ttttatttta ggaagccatt gttttattgc
     2461 agtattttcc gattctaaaa gatcaattaa atcatttaag ttcgcaatag caataacagg
     2521 cacgcttaat tttacagata cttcagcaac agcagaaagg ttttctctac ctctttcttg
     2581 tctgtccaat gatattaaaa tacccgctaa tgtaccacca aggtcagtaa tcaatctagc
     2641 gcattcagaa accgcagtcc ctgcggtaat cacatcatca acaattaaca ctctaccttc
     2701 gataggagct cctattatca aacctttttc accatggtct tttgtttctt ttctattgaa
     2761 ggcaaatggg atgtctattt tataatctct tgctaaagct gaagacaaca cagttgtaat
     2821 tgaaattcct ttataagcag gaccaaacag catgtcatac tccagcgtac ttgccattaa
     2881 agctgcagca taaaaccttc ccaaacaatc tatagattga cctgaattaa acagccctaa
     2941 gtcaaaaaaa taaggactaa ttcggcctga tttaagttta aattctccaa attttaaaac
     3001 acctttagtt aaagcaaatc ggataaattc ttgtttgtta ttttccatgc ggtataaaat
     3061 tatctggtag ttgcatagtc tatcatctca tttcatttgt gtagcaatga gatttgtgaa
     3121 aactttttta ttaacattta ggcaactgga ggatgacata agaataatta ctgtaaatgt
     3181 gaatggaatc agagcagctg cgagaaaagg tttttttgaa tggttgccaa aacaaggcgc
     3241 tgatattgta tgtcttcaag aaatcagagc tcaactggac gatatccaaa gtgaaacatt
     3301 ctggccagag cagtaccatt gtttttattt tccagctgag aagaagggat acagtggggt
     3361 agctattttt tcaaaaaaga agcccagcag tattcttgaa gggttaagtt cacaagagtt
     3421 tgattctgaa ggtcgctatc tagaatgtgt ttatgacgat tttacaatag catcagttta
     3481 ttttccttca ggctcttctg gtgatatcag gcaacaaatg aagtataggt ttttagatgt
     3541 ttttgaagac aaactaaaag acaagcttca atccagaaag ccctatattt tttgtgggga
     3601 tgtgaatatc gttcataaag aaatagatat taaaaattgg aagtctaatc agaagaactc
     3661 aggttgtttg ccagaagaaa gagcatggct tgataaaata tttgatcaat tatgctttgt
     3721 tgattcattc cgggtagtca atcaaaatgc tgaccaatat acatggtggt ctaacagagg
     3781 aagggcttgg gaaaagaatg tgggatggag gattgattat caaattacaa ctcaaaattt
     3841 taaaaactgc gtgaacaaat cctttatcta tagaaaaaaa cgtttttcag atcatgcacc
     3901 attggtgatt gattatgatt accctttcta aaaatgataa ataaacaaca acacggtctt
     3961 gggatttatt taaaaccaga ggttattaga atggttttct taggattctc tgcaggttta
     4021 ccataccctt tgctttttgc aacattgaca gtctggttag cctcggtgga cataggaatt
     4081 gcagaagtaa gcatgtttgg atgggttggt gttatctatg caatgaaatt tttatgggct
     4141 ccaatgcttg atagtctttc catacctttt ttatcgaaaa ctttagggca aagacgagca
     4201 tggatgttgt tgatgcagtg ttgcattcta atcggtttgt tttatatgtc tttttttaaa
     4261 ccccaaacta accttagttg gttggcttat cttgcaatca ttattgcttt tgcatcagcc
     4321 acacaagata ttgcaataga tgcttatcga attgagatta ttagtaaccg attccaagga
     4381 gcaatggcag caagctatca actgggctat agaatctctg tattgttgtc aggagctggt
     4441 gcattatata ttgctgaatt ttattcttgg aaactggcct atcaaactat ggcattattt
     4501 atgttagttg gcgtcgcaac tgttttaatc atttctgagc ctaaggagca aaaatcaagt
     4561 caatactcaa tccattggtt taaggaatca gtcattcatc ctttttcaga attttttacc
     4621 agaaatggtt actggtccat tatgcttttg ttgtttattg ccttgtatcg tgtcagcgat
     4681 ttaatgctca atatagtggc caaccctttt tatattgata ttggctttaa actcagtgaa
     4741 attgcgtcaa ttacaaaagt gtttggtttt attgttacaa ttattggtgc atttgttgga
     4801 ggtctgtccg ttgccaagta tggtatcagc aaaccattga tagtaagcag tatattactt
     4861 gcaggaacca atttattctt tttattacta gaaagcttag gagcagactt aaggattcta
     4921 atattaacaa tcagtgctga taattttgca ttagggtttt ctggctctgt ttttattgcc
     4981 tttctatcca gcttaaccaa taggaattat actgcttcac agtatgcgct cttttcttca
     5041 ctgatgtttt tacctggtat cactttaagt ggcttttcag gacaatttat tgaatctgtg
     5101 ggctggtcag attttttctt gtttgccgct gccatgggcc tgcctgcaat tgtatttgct
     5161 gtgataattg ttaagaaagg atggcaagaa cagtaaaaaa attaagcatt aaaaacaaat
     5221 gaatattctt gaataaactg ttctaaagag cacggcgatt ttcccattaa atcctgaaaa
     5281 gtattagtct tgtcctcgat accacctgct gcgatacctt tgaaaaggtc aatcacagaa
     5341 tccaaatgcc actggtttgt taagaactga gacaatgtct ttttatattc gtctataggc
     5401 acgttgatat aatcaacttt cttaccaagc gttctgctga atatctcagc aacatcatga
     5461 aaggataaaa tttctggtcc tgtaacttca taattttgtc ctgcatgacc atcttccgaa
     5521 agcactttag caatcaccat tccaatatct cttgtatgaa tcattccagt tttaccttca
     5581 cccataggca agaaaaattt atcctgctca acaattgttt taccggaacc taataagttt
     5641 tgcataaaga aatttggttt aatcattgtc cagctcattc cagaatgttt tatgtaattt
     5701 tctgattgca tatgaagttt aggaattggg ctagttgcct caggcacagc ttccatggag
     5761 gatattttta caatatgttg aacgttggct gcaagtgcag tatcgactat tttcttttct
     5821 agaaagagtt gttgttcgga attgggtaac aataccaagg ttttttgaac tcctttcatt
     5881 gcttgattta aagccccatc atcttctata gcgccaataa taacttcacc acctaggtca
     5941 gttatttggc tttgtttatc cttattccta attaatgccc taaaaggaat atttcgactg
     6001 gacagttctt gaacagctgc tgaacctgta ttgccagtcg ctccagttat taagatcata
     6061 ctattctcct ttggtaattt ttttcatcaa taattgatgg aaatgtgcaa cgcccgattc
     6121 gtatttagag cttaaaggac ccgattgata tattcctgcc tgtaaatttc tttggatttc
     6181 gggtacaagc tgctgatctt ctaaactcac atcaagttga tacttcccct cactagattc
     6241 aaaatcatcg aatgagacac ttgatggtct aaataaatta taaaccaccc tacaccactt
     6301 tgcttgaatg ggttcaattc tttgaaacac gattcccctt ccgtatacat taaaaataaa
     6361 gttgggaaag tgccaaccaa aacttccagg ttgattgcta ccaagtctag ctggaacaga
     6421 atgaatgctg cataattctg tattttctat ggtgtaatct tcccaaacaa catccctgtt
     6481 taattgtggg tgtactaaag ggatatgata accctcctga tagttgtcaa cataggtttt
     6541 ccagttggca tgaacatcaa atttaagctc attatgaaaa atataatcat tgtcatagta
     6601 gtcattaatt accttgttaa agggtcctag ccagtcattt agagattgcg attttaaact
     6661 aaaattcaca aagattaacc catacttttc tgagactcta attggaaaaa gtgaatgttt
     6721 tttataatta aaatctttac caccaaaaaa attgggagga ttttttaagt tgccatccaa
     6781 gccataattc catccatggt aaggacatat catgctttga cagttacctc tattttgttg
     6841 taaaacctta gatgcccggt gtctgcagac attatgaaat ccattgattt tattgttttt
     6901 gtctttaatt aacagaatag gataa
//
LOCUS       DASCTH010000119         2047 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000168687, whole genome shotgun sequence.
ACCESSION   DASCTH010000119 DASCTH010000000
VERSION     DASCTH010000119.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000168687"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            179..1249
                     /locus_tag="QGH35_02495"
     CDS             179..1249
                     /locus_tag="QGH35_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJL81436.1"
                     /translation="MDIEINLEPDLHPDEFAELAVAAEEYGVRAIWSSSYHQNWDPFL
                     SLVPAIQKTSKILLGPLAISPFEMHPLKMANAVLTLNELSNGRAMIAVGAGGGVLGAM
                     NYKCPQGNCHGLKPFRIIRAVREAVEILHLAAGKKFNLKYEGEIFTIPRPFMQGFTWA
                     KSPPPKIYTCSTEPQMLRLGAQCADALQMSDVALPMLDEAMDNIKIGLERRETPTEDF
                     RVGNFWAWHIKDDKEASYKEARRELIYRGSLLPPFSLQHFLSKDEAQIVIDNWNSGAN
                     NEFIKAYISRSGEIKNVPETLVSKLVAALSSAGDMNDIDRELERFEVFKKAGLTDLVI
                     RLFDEPMKGLKMIGERVLPALR"
     gene            1354..>2047
                     /locus_tag="QGH35_02500"
     CDS             1354..>2047
                     /locus_tag="QGH35_02500"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HJL81437.1"
                     /translation="MKHYRKQRFLCDFIKKNQETMILVGDTAHHLTRVLRLKTGDKII
                     VFDQSGYEWDAKISNKKRKSVEIELLDRHKPDVESPVNITLLQSISRSHRMDLVMQKA
                     TELGVNSIVPMLTKNMVVKLNAKNTQKKMNHWEKITISAAEQSGRVHLPELLEPRELS
                     EDLLTEHKKEASFFLDTTGSENFSGNVIKNISIAIGPEGGFNRDEKNMAEKSGYRIIK
                     TGPRLLRTETAPI"
BASE COUNT          690 a          343 c          442 g          572 t
ORIGIN      
        1 ctgagagaga gttgtgcaaa cttgcagatg aaaaagaaat taatcaagag tcgttcaaat
       61 acctaaatag attatcagat gctttgtttg tcattgccag gaccttcgca aaacaagaca
      121 atggggaaga gatcatttgg aagcacccaa aggtttaaat atttagaggt agattattat
      181 ggatatagaa ataaacttag agccagattt gcatcctgat gagtttgctg aacttgccgt
      241 agctgcagaa gaatatggag tgcgggctat ttggtcatca agttaccacc agaactggga
      301 tccttttttg agtttagtgc cagcaataca aaaaactagt aaaattctct taggtccatt
      361 ggctataagt ccttttgaaa tgcacccgtt aaagatggca aacgctgtct tgactctcaa
      421 tgagttatcc aatggcaggg ctatgattgc tgttggtgct ggcggtggtg ttttaggggc
      481 aatgaactat aagtgtcccc aagggaactg ccatgggcta aaaccttttc gtataattag
      541 ggcagttagg gaagcagttg agattttaca tcttgctgca ggcaagaagt tcaatctaaa
      601 atacgaaggc gaaattttca ccattcccag acctttcatg caagggttta cctgggccaa
      661 gtcacctcct cctaaaattt atacctgttc aacagaacca caaatgttgc ggttgggcgc
      721 tcaatgtgct gatgctttac aaatgagtga cgtcgccctg ccaatgttag atgaagccat
      781 ggataatatt aagattggtc tggaacgcag agagactccc actgaggatt ttagagtggg
      841 aaacttttgg gcttggcaca tcaaagatga taaagaagca tcttataaag aagccagaag
      901 ggaattgatt tatcgtggct cattactacc tcctttctca ctccaacatt tcctcagcaa
      961 agacgaagca caaatcgtga ttgataactg gaactctggt gcaaataatg aatttattaa
     1021 ggcttatatt agtcgttccg gtgaaattaa aaatgttcct gaaacgcttg tttctaaatt
     1081 ggttgcggca ctcagttcgg cgggagatat gaatgatata gatcgagaat tagaacgctt
     1141 tgaggtattt aaaaaagcgg gtctcactga tctagtcatc cgattgtttg atgagcctat
     1201 gaaaggactg aaaatgattg gagaaagagt actgccagca ttacgataaa cctaatactc
     1261 ccaattgata ggagtaaaag gaaaatttta aatacagttg tcattaccat gttaatgtct
     1321 gtatagatat taaccagatt gaaattatta aagatgaagc attatcgaaa acaacgcttt
     1381 ctgtgcgatt tcattaaaaa aaatcaagag acaatgatct tagtaggtga tacggctcat
     1441 catttaacgc gcgtcttgcg tttaaaaact ggggataaaa tcattgtttt tgatcaaagt
     1501 ggctatgaat gggatgctaa gattagtaac aaaaaaagaa aatctgttga aatagaattg
     1561 ttggatcgtc ataagcctga tgttgagtct cctgtcaata taacgctttt acaatcaatc
     1621 tccaggagcc atcgaatgga tctagtgatg caaaaagcaa ctgaattggg ggtcaatagt
     1681 attgtgccta tgttaactaa aaacatggtt gtcaaattaa atgctaaaaa tactcagaaa
     1741 aaaatgaatc attgggaaaa aataaccatt agtgctgcag aacaatcagg aagagtacac
     1801 ttgccggaac tattggaacc cagagaatta tctgaagatt tgttgactga acataaaaag
     1861 gaagcatcat tttttctcga caccaccggg tcagaaaact ttagtggaaa tgtaataaaa
     1921 aatatttcaa tcgccatagg tccagaagga ggttttaatc gtgatgagaa aaatatggca
     1981 gaaaaatctg gttatagaat tattaaaact ggacccagac tattgagaac agagactgca
     2041 cccatta
//
LOCUS       DASCTH010000120         6497 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000168938, whole genome shotgun sequence.
ACCESSION   DASCTH010000120 DASCTH010000000
VERSION     DASCTH010000120.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6497)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6497)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6497
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000168938"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="QGH35_02505"
     CDS             complement(<1..2003)
                     /locus_tag="QGH35_02505"
                     /inference="COORDINATES: protein motif:HMM:NF039587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HJL81438.1"
                     /translation="MTQKIKQNSFLSLLELLFFVFLGFNPIHAEIVIDGELSEAEWNQ
                     AQVIEDFVVTRPFSLEAPTYKTKVLIYSDLEGIYVGFVNQQPMETRDRRRHNRDMLKQ
                     DYDRNIVVIDFDNKGNSAYMLGVSLSESLIDFTITNESETDGDWDGEWFAKTSESEEN
                     WYSEFFIPWTMVSMNQQKGPNRKIGISTSRLIQHEAKYVAFPQASPLRKKFLSLLHTI
                     EVVQSNPRRLDFYPYLVSKGNFVDDDMSYQAGSEIFFANGSGGELSVTLNPDFGQVES
                     DNIVINYSARETFYSDKRPFFTQSQSLFEISNEWYPGFELYSIIHTRRIGARPDYDCS
                     KYGSDSGGSDEDELQCLSSQEMNNDIDVALKYTQLGDKTDFGFFGAFEHDEDFSEGKD
                     FFAVRTSHRAGNHKIGHMLTHVDRSAIDRDATVNAFDYQFTPNDIIKFDSVLMFSDSN
                     QEKSGFGSRNAVVFEHNKKWRSGAEFYYYNDKLNIDDMGYLYRNNLFSGGANVSYTRT
                     DFTKDSPVLSRSYSWDFWSENNADGETIDNSIVLFFMQQFKDTSSIHLNIFTNSEGID
                     DDVTRGSLSAPFLESGKGLATDFNYNTRIIGKWQADLNVYAQKKDRLSFTERTRRVST
                     GLRYFPSDNLRFYLQLSHTKVNNWFIWRSDNHYATYQSRNTTI"
     gene            complement(2007..2981)
                     /locus_tag="QGH35_02510"
     CDS             complement(2007..2981)
                     /locus_tag="QGH35_02510"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HJL81439.1"
                     /translation="MTEIDKVMYVFPGQGSQYPGMGQDLYHSFETAKVIFEQANDILN
                     YDLVKIAFTDPDDQIGLTRYTQPILLTHQMACFRVFQELTSSKITPVLAAGHSLGEYT
                     ALIVAGVLSFEDGLKLVSKRGELMGEYGQGSMLALPLMGDEAQLIAEDHGCQVATMNL
                     PQQTVVGGKDSDIEKLESTFAELYPRKRAVKLDTEGAFHTNLMKTAALAFKETLERAS
                     FAPTTMGVVSNFTAELHAEDGSATKNLLFQQLFKPVNWVGCLKTSVSFGIQTIIEFGG
                     GIGKELEPENKRPNLESIIKKNFRALEYPIDYYPAINLKTVEESARSF"
     gene            3134..4033
                     /locus_tag="QGH35_02515"
     CDS             3134..4033
                     /locus_tag="QGH35_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81440.1"
                     /translation="MSHVKEVFYKPPYVGILAFLIVFLTQGLGHTLMVLVEKTLGADY
                     QYQGAFYLGILGGILLFIGMKKENEVPATWLGYFAGLFLWTGWVEFSFVFYAEYLEVE
                     QILPDGSLNRFPEYLLMQSSIGVLLTTLLYFLLNRETKCNFFKWIQRNFKLSTGKPTP
                     GYPRNFAAITALETIYVIWFFYIILLLLYEDAFIGAYHPITYAFFVLNTTWAVYLLWR
                     LFKFWKVTTAIRYAIPTAIIAYSSYEVIGKWGLLVEFWVYPQEYLTEILVVLGTVIMI
                     TLIAVFTPTGFKQKLLNQNKENN"
     gene            4145..5254
                     /locus_tag="QGH35_02520"
     CDS             4145..5254
                     /locus_tag="QGH35_02520"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HJL81441.1"
                     /translation="MFINFWYPAETSGNLTDQPIRKKMLGQNFVLFRDQSGKPRCLSD
                     VCIHRGGSLAGGKIKGDCVECPYHGWQFDGEGICHRIPSLGKNAKIPKRAKIDSYPTK
                     EIYGLIFCFLGDLPEEERCPILEVKEYEMEGWRTTWQSLQFDIDYKRSIENGIDAAHN
                     EFVHPSHGFSGENEDYKAPEIDITSTEWGTGLFSKMFAPPLKEKKMQKASGRKENALI
                     EAGTGHHGISTLWTYIHPTPEVFIHQYAFETPIDETQTNFYLITTRNFLTDPKDDDRV
                     IERNQVVAFQDRDVLLGVKPLLTPNESNNEFFVPIDKPIKLYRELLKKWEEKGWRIDT
                     ETVRENKLSHAYAIPSPARKKAKGWVIDSVPLLKS"
     gene            5357..6469
                     /locus_tag="QGH35_02525"
     CDS             5357..6469
                     /locus_tag="QGH35_02525"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HJL81442.1"
                     /translation="MYINFWYPVCLTNELEDKPIKRKILGVEFVAFRDQKGIAHCLSN
                     VCVHRGGSLGDGKCLNNAVACPYHGWQFDGDGKCITVPSIGYNEKPPARAKVDSYPVI
                     EKYDIIFAFLGDLSEEERPGLYEIEEYEAEGWRANETITIDIDYNYERSIENGLDPAH
                     NEFVHPTHGFSGVKNDYKVNPTKISQTQWGANFSQVFDAPPLDKKTYGETARDESGEL
                     EVLGGYHGPNALITQIHTKKETGWFHQYFFEKPIDEFHTQIFFVNMRNWLMEESMDAM
                     VTERNLAIAKEDITILNVIQPVVTPPTMNKEILVPSDAVVGEYRKTLAGFKSKGFKID
                     TKKLKEEEHQTAYSVPCPARNTEKNWVLDPIPLLKN"
BASE COUNT         1968 a         1311 c         1261 g         1957 t
ORIGIN      
        1 ttaatcgtgg tattccttga ttgataagta gcatagtggt tgtcgctcct ccagataaac
       61 cagttattaa ctttagtatg ggatagttgc aaatagaatc ttaaattgtc ggaaggaaaa
      121 taacgtagtc ctgtcgatac acgtctggta cgctcagtaa aagaaaggcg gtcctttttt
      181 tgtgcataca catttaaatc tgcttgccat tttccaataa ttctcgtgtt gtagttaaag
      241 tcagtagcca gtcctttgcc actttctaga aatggtgcgg ataaactgcc acgcgttaca
      301 tcatcgtcta ttccctcact attagtaaat atgtttaaat ggatgcttga tgtgtctttg
      361 aattgctgca tgaaaaacaa tacaatagaa ttgtcaatgg tttctccatc tgcattgttt
      421 tcactccaaa aatcccaact atagcttcga ctcagaaccg gcgaatcttt ggtgaaatcg
      481 gttctggtat agctcacatt tgcccctcca gagaacaaat tgtttcgata caaatatccc
      541 atgtcgtcaa tattgagttt gtcattgtaa taatagaact cggcaccgct tcgccatttt
      601 ttgttgtgct caaaaacaac agcgtttctt gagccaaatc cactcttttc ctgatttgaa
      661 tcagagaaca taagaacact gtcaaatttt atgatgtcgt ttggggtaaa ttgataatca
      721 aatgcattga cagtggcatc ccggtcaata gcacttcgat caacatgggt caacatgtgg
      781 ccgattttgt ggtttccagc tctgtgtgag gttctaactg cgaaaaagtc cttaccctca
      841 ctaaaatcct catcatgctc aaaagcaccg aagaagccga aatctgtttt atcgcctaat
      901 tgagtatatt tgagcgccac atcaatatcg ttgttcattt cttggctgct taaacattgc
      961 aactcgtcct catcactgcc acctgagtca gaaccatact ttgagcaatc ataatctgga
     1021 cgagcaccta ttcttctggt gtgaatgatc gaatatagtt caaaaccagg ataccattca
     1081 ttggaaattt caaacaggga ctggctttga gtaaaaaatg gccttttatc agaataaaat
     1141 gtctctcgtg cagaatagtt aatgactatg ttgtcgcttt caacctgacc gaagtcaggg
     1201 ttgagagtca cgctaagttc accaccgcta ccattggcaa aaaatatttc ggaacccgcc
     1261 tgataggaca tatcgtcatc cacaaaattg cccttactta caagataggg gtaaaaatca
     1321 agtcgtctcg ggttggactg aacaacttca atggtgtgca acaaggagag aaattttttc
     1381 ctcagaggtg atgcctgcgg aaaagcaacg tattttgcct catgttgaat cagcctactg
     1441 gttgaaatac cgatttttcg atttggacct ttttgctgat tcatcgatac catggtccaa
     1501 ggaatgaaga actccgagta ccagttttct tctgattctg atgttttagc aaaccattcc
     1561 ccatcccaat ccccatcagt ttcactttca ttagtgatag tgaaatctat taacgattcg
     1621 cttaaggata cgcccaacat ataggcactg ttgcctttgt tatcaaaatc gatgacgacg
     1681 atattccggt cgtagtcttg tttgagcatg tcgcgattgt gtcttctccg atccctggtc
     1741 tccatgggtt gctgattaac aaagccaacg taaatgcctt ccaggtctga atagatcaaa
     1801 acctttgttt tgtatgtggg agcttctaga ctaaaaggtc tagtcacaac aaaatcttcg
     1861 attacttgtg cttgattcca ctcagcctcg ctgagttctc catcaatcac tatttctgca
     1921 tgtataggat taaaaccaag aaaaacaaaa aacaataact ccaaaagaga tagaaaagaa
     1981 ttttgtttta ttttctgcgt cacctgctaa aaagatcgtg ccgactcttc aacggtcttt
     2041 aaattaattg ccggatagta gtcgataggg tattcaagag ctctaaaatt cttcttaata
     2101 atcgactcaa gattgggtct tttgttctct ggttcaagct ccttaccaat gcctcctcca
     2161 aactctatga tcgtttggat accaaatgag acggaagtct ttaaacaacc cacccagttt
     2221 accggcttaa acaattgttg gaacaataga ttcttagtag ccgatccatc ttccgcgtgt
     2281 agttcagcag tgaagttgga gactacgccc atagtagttg gagcaaaact agctctctcc
     2341 aaggtttctt taaatgccaa tgctgctgtt ttcattaaat tcgtatgaaa agcgccttca
     2401 gtatcgagtt ttacagctct ttttcttgga taaagttcgg caaaagtaga ttccaatttt
     2461 tcaatatcag agtctttgcc tcctactacg gtctgctgcg gcagattcat agttgcaacc
     2521 tgacagccgt gatcttccgc aatcaattga gcctcatccc ccatcaaggg cagcgctagc
     2581 atagaaccct gaccgtattc acccatcaat tcacctcgtt tgctgaccaa cttcagaccg
     2641 tcttcgaaag ataaaacccc ggcaacaatt aatgctgtgt attctcccaa actgtgaccg
     2701 gcagctaaaa caggtgtaat ctttgagctc gttaactcct gaaataccct gaaacaagcc
     2761 atctgatgtg ttaagagtat cggttgggtg taacgagtga gcccaatctg atcatctgga
     2821 tctgtaaatg caattttaac tagatcatag tttaaaatat catttgcctg ttcaaagatt
     2881 acctttgccg tttcaaaact atggtacaag tcttgtccca tccctggata ctgcgatcct
     2941 tgtccaggaa aaacatacat aactttatct atctcagtca tgatttctcg atattataat
     3001 aggctgaatt ttatcatcat ttcaaaatat agaatgcatt gtattcgaac tttcctataa
     3061 tctatatatt aagaattcac caatgtatca acaaccaatt aaaatgtgac ctaaccggtg
     3121 ttagaatagg acgatgagcc acgttaaaga agtattttat aaaccgcctt atgtaggcat
     3181 attggccttt ctaatagtat ttctaactca gggtttggga cacactctga tggtgcttgt
     3241 cgaaaaaact cttggcgccg actaccaata ccaaggggca ttctatcttg gaatattggg
     3301 aggaattctc ctgtttatag ggatgaaaaa agaaaatgaa gtgccagcaa cttggttggg
     3361 ctattttgca ggtctttttc tgtggacagg ctgggttgag ttttcctttg ttttctatgc
     3421 agaatatctt gaagttgaac aaattcttcc cgatggcagt ctcaatagat ttccagagta
     3481 cttattgatg cagtcctcga ttggggttct attaacaacc ttgctctact ttttattgaa
     3541 tcgagaaaca aaatgcaatt tcttcaaatg gattcagcga aattttaaac tatcaacagg
     3601 aaaaccaact cccggctatc caagaaactt tgcagccata accgcactcg aaacgatata
     3661 cgtaatttgg tttttctaca taattctttt gctgctttat gaagatgcat ttattggtgc
     3721 ttatcatccc attacctacg ccttctttgt tctaaatacc acttgggctg tttatctatt
     3781 gtggagatta tttaaatttt ggaaagtcac taccgctatc aggtacgcca ttccgaccgc
     3841 aatcatcgca tacagttcct atgaagtgat tggcaaatgg ggattattgg tagaattctg
     3901 ggtatacccc caggaatact taactgagat tctagttgtt ttaggaactg tgattatgat
     3961 caccctaatt gcggtcttta cacctacagg atttaaacag aaactgctca atcagaacaa
     4021 agaaaataat taatttagtg aaaaccgatt attttggtaa tccttgttaa ttgatctata
     4081 tcattgttga attgtaattt cttagatagg ttagattgct aaaatttgaa taggggactt
     4141 cattatgttt atcaatttct ggtatccagc tgaaaccagt ggcaatctca ctgaccaacc
     4201 cattcgaaag aaaatgcttg ggcagaactt tgtcttgttt agggatcaat ctggtaaacc
     4261 aagatgtttg agcgatgtat gtattcatcg gggaggatcc ttagctggtg gcaaaataaa
     4321 aggcgactgc gtggagtgcc cttatcatgg ctggcaattt gatggtgaag gaatatgcca
     4381 tcgaatccct tcattgggaa aaaatgcaaa aataccaaaa agagctaaaa ttgattccta
     4441 cccgaccaaa gaaatttatg gtctaatctt ctgttttctc ggcgaccttc cagaagaaga
     4501 aaggtgtccc attcttgagg tcaaagaata tgaaatggag ggatggagaa caacgtggca
     4561 atctctgcaa tttgatattg attataaacg atccattgag aatggtatcg atgccgcaca
     4621 caacgagttt gtccatccta gccatggttt ttcaggtgaa aatgaggact ataaagctcc
     4681 tgagattgat atcacatcga ccgagtgggg aactggatta ttcagtaaaa tgtttgcccc
     4741 tcctttgaaa gagaaaaaaa tgcagaaagc ttcgggccgc aaagagaatg cactcattga
     4801 agccggaacg ggtcatcatg gcatatcaac gctttggact tacattcatc caacacctga
     4861 ggtttttatt catcaatatg cgttcgaaac accaatcgat gagacccaaa ctaatttcta
     4921 tctcattacc accagaaatt ttctaactga tcccaaagat gatgatcgag tgattgaacg
     4981 caatcaagtg gttgcttttc aagaccgaga tgttctgtta ggggtcaagc cactattgac
     5041 gcccaatgaa agtaacaatg aattttttgt tcccattgac aaacccatta aactctatag
     5101 agagttgttg aaaaaatggg aagagaaagg atggagaatc gatactgaaa ccgttagaga
     5161 aaataaatta tcacatgctt acgccattcc cagtccagct agaaaaaaag ccaagggttg
     5221 ggtcatagat tctgttccac ttttgaaatc atagtcacaa ctcactatgc ttgttacaaa
     5281 taattactta tccggcttaa attattagcc tgatacgaat ataatcaatc tggatcagat
     5341 taatagggga aaataaatgt atatcaattt ttggtatccc gtttgcttaa ccaatgaact
     5401 ggaagataaa ccgatcaagc gaaaaatact tggtgttgag tttgttgctt tcagggacca
     5461 gaaaggcata gcccactgtc ttagtaacgt ctgtgttcat cgtggaggtt ctctaggtga
     5521 tggaaaatgc ttaaataacg cagtcgcctg tccataccat ggttggcaat ttgatggcga
     5581 tggaaaatgt attaccgtgc catctattgg ttacaacgaa aaacctccag caagagcgaa
     5641 ggttgatagt tatccagtga ttgaaaaata tgacatcatt tttgcctttc tgggtgatct
     5701 ttccgaggaa gaaagaccag gactctatga aattgaggaa tatgaagctg agggatggcg
     5761 tgctaatgag actattacca tcgatattga ttataattat gaacgctcca ttgaaaatgg
     5821 attggatccg gcacacaatg aatttgtcca tccaacccat ggattctcag gtgtaaaaaa
     5881 cgattacaaa gtcaacccta ctaaaatatc tcaaactcaa tggggagcaa atttttctca
     5941 agtctttgat gctcctcctc tggataagaa aacctatggt gagactgcaa gagatgagtc
     6001 tggtgaactc gaagttttag gcggatacca tggtcctaat gctttgatta cacaaattca
     6061 taccaaaaaa gaaactggtt ggtttcatca atattttttc gaaaaaccaa ttgatgagtt
     6121 tcacactcag atattttttg tcaacatgcg taactggctt atggaggagt ctatggatgc
     6181 gatggttaca gagagaaacc ttgctattgc caaagaagac attacgattt taaatgtcat
     6241 tcaacccgtt gttacccctc caaccatgaa caaagagata ttggttccat cagatgctgt
     6301 tgttggcgaa taccgaaaaa cactggcagg attcaaaagc aaaggattta agattgatac
     6361 aaaaaaactg aaagaagagg aacatcaaac cgcatattct gttccttgcc cagctagaaa
     6421 cactgagaaa aattgggtat tagatcctat tcctctgctg aagaactaaa tcaacagtta
     6481 cacctttggc tctggaa
//
LOCUS       DASCTH010000121         5628 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000169088, whole genome shotgun sequence.
ACCESSION   DASCTH010000121 DASCTH010000000
VERSION     DASCTH010000121.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000169088"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="QGH35_02530"
     CDS             complement(<1..410)
                     /locus_tag="QGH35_02530"
                     /inference="COORDINATES: protein motif:HMM:NF028500.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HJL81443.1"
                     /translation="MSADLLDLKGVGPALTTKLEKLNVYSQSDLLFLLPIRYEDRTTI
                     KKIGTLVAGEKALIEGRVLLSNVLYRGRRMLVCQLSDDTGIVALRFFYFSRQQAKKLK
                     RDAIIRCFGQTRKAQAGLEIIHPEYQIIDPENPAP"
     gene            complement(427..825)
                     /locus_tag="QGH35_02535"
     CDS             complement(427..825)
                     /locus_tag="QGH35_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HJL81444.1"
                     /translation="MKKHLHTKNSPAAIGPYSQAVLVDNLVFLSGQIPLNNKGKMVEG
                     TVEQQAQQIFTNIEAILVEAGGTLNDLVKLTVFLTSLDDFEVINKIMMLRLTRPYPAR
                     SCVEVSALPKGSSVEVEAIARISLHTKLHT"
     gene            complement(848..3001)
                     /locus_tag="QGH35_02540"
     CDS             complement(848..3001)
                     /locus_tag="QGH35_02540"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HJL81445.1"
                     /translation="MEEFADKAAAMMSKKLPKLMRQLHDNLHYLGQSDQEFVKKAYVF
                     ANIAHEEQLRRSGEPYINHPIAVAKTLADLRLDKESIAAGLLHDVLEDSTINPDLIVK
                     EFGQNVLNLVDGVSKLDQLEYSENESTQAESFRKMMLAMIEDIRVILIKLSDRLHNIQ
                     TLDALDKNKQQKIARETLDIYAPIANRLGIYHMKTALEVDAFKHAYPFRQRILGNALK
                     RRTGHQKKILRTIASRFRKNLDEARIDAEIQSRKKDLYSIYKKMKNRKLPFDQIIDVY
                     GIRIIVEQVEECYQVLGLVHQIYKPIMDKFNDYIAIPRINGYQSLHTSLLGPDGTPIE
                     VQIRTKAMNRVAESGIAAHWKYKTEEKDAVPPQIRAREWLATIEEIQGATHPEEFLES
                     IKVDLYPDKVYVFTPKGKILRLPINSTCVDFAYAVHTDVGNSCIGAKLDRQQVPLRTI
                     VQSGQTVEILTSKQSNPDPSWLNFIATAKARLNIRAYLKNLNVAETIALGKRLFNHAL
                     TILGKKQRNISRKQLKNFLNDLGLKDSKDLYESIGTGDKNPILMAQLLLGESESDDTM
                     KEDNPLYIKGTEGVAVSFPKCCTPIPGDTVIGHLSKQRGLVVHRRNCRHVSSFIKDTS
                     RWIGVEWSERLEHSLSTDINVNIIHKPGSLAEVASTIADKGCNVEQVTILDEHEDETI
                     DLLFSVQVKSRLELAEVMRAIKKIKNVIKITRRLH"
     gene            complement(3156..3416)
                     /gene="rpoZ"
                     /locus_tag="QGH35_02545"
     CDS             complement(3156..3416)
                     /gene="rpoZ"
                     /locus_tag="QGH35_02545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HJL81446.1"
                     /translation="MARVTVEDCLEHVDSVFDLVKLASKRARRIADGADTLLEDDEND
                     KPVVLALREISEGLMDNPEFHTSENALEKEIAEELARSLNEE"
     gene            complement(3471..4085)
                     /gene="gmk"
                     /locus_tag="QGH35_02550"
     CDS             complement(3471..4085)
                     /gene="gmk"
                     /locus_tag="QGH35_02550"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HJL81447.1"
                     /translation="MVVSAPSGTGKTTIVKKLISQRANFVASTSYTTRAKRANETDGV
                     DYVFIDQQLFTEMINKNLFVEHAKVFDHYYGTPKKEISQAQKKGQTVFLEIDWQGAAS
                     IRKTNPDCMSIFIVPPSKQELKKRLTNRATDSADQIVHRYKLAVQDIEQYIHFDCVII
                     NKDIDSTCLEIMGLLDSSQNIKHNISNEALTIIESFIGPQIETN"
     gene            4180..4767
                     /gene="rdgB"
                     /locus_tag="QGH35_02555"
     CDS             4180..4767
                     /gene="rdgB"
                     /locus_tag="QGH35_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011219718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HJL81448.1"
                     /translation="MKTIVLASNNANKAKEIQMLLGDQVEIIMQQDLNIESIPETGKT
                     FEENACLKATTAYKKAKLPVIGDDSGLEVDCLNGQPGIHSARFAGDGASDKDNITKLL
                     DQMTGVPLEQRTARFKCVIALIGLEELDKVNFFHGEWNGFIAEQVTGHNGFGYDPVFV
                     DPGTQLTAAQLDQGLKNKLSHRGQAIDAVKNYLSF"
     gene            4768..>5628
                     /gene="hemW"
                     /locus_tag="QGH35_02560"
     CDS             4768..>5628
                     /gene="hemW"
                     /locus_tag="QGH35_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HJL81449.1"
                     /translation="MHNAATHPTSLYVHFPWCQSKCPYCDFNSYKLNKRLPASEYVST
                     LIVDLKKTLTFSNSSLELKSIYFGGGTPNLFPPDAFKTLLDNIHEECNFTDDIEITLE
                     MNPGIGQPDNLERYLALGINRLSVGAQTFSKKILNNIGRLHNENQTIQLYKQAKKTGF
                     ENINLDLMFGLPGQTSSQFDHDLRQLVDLSPEHISFYELTIEAGTQYYLKKPDLPDHE
                     IIWSMHDQGKNVFEQAGYQQYEISAFSKPGFQCVHNLNYWLYGDFVAIGAGAHGKYTG
                     PTRDIFRYVKK"
BASE COUNT         1579 a         1118 c         1119 g         1812 t
ORIGIN      
        1 ggtgctgggt tttctggatc tatgatctga tactccgggt gaatgatttc caacccagcc
       61 tgtgcttttc tggtttggcc gaaacaacgg atgatcgcat ctcttttgag ttttttggct
      121 tgttgtctag agaagtaaaa aaaacgtaaa gcaacaatac cagtatcatc ggaaagctga
      181 caaactagca tcctacgccc acggtagagc acattgctga gcaatacccg cccctcaatt
      241 aatgcttttt cgccggcaac aagagtgccg atttttttta tagtggttct atcttcatag
      301 cggataggta gtaaaaacag caaatccgac tggctgtaga catttagttt ttcaagcttt
      361 gtagttagag cagggccaac cccttttaaa tctaataagt cggcgctcat ctcggaggta
      421 tcaacatcaa gtgtgtagtt tggtgtgtaa gctgatccgc gctatagctt caacctcaac
      481 gctagaaccc ttgggcaggg cactgacttc tacacaggag cgtgcaggat acggccttgt
      541 caaacgcaac atcatgattt tattaatgac ttcaaagtca tcaaggctgg ttaagaaaac
      601 agttagtttt actaaatcat tgagtgtgcc tccggcttcc acaagaatgg cttctatgtt
      661 tgtaaaaatc tgttgggctt gttgttcaac cgtgccttct accatttttc ctttattgtt
      721 gagaggaatt tgtcctgata gaaagaccag attgtcgact aaaacagcct gggagtaagg
      781 acctattgct gcaggtgaat tttttgtatg caagtgtttt ttcatcaagg ttggccgtta
      841 atttttttta atgcagccgc ctagtaattt ttatgacatt ttttattttt ttaattgctc
      901 tcatcacttc cgccaactct aaacggcttt ttacctggac cgagaataat aaatcaatgg
      961 tctcgtcttc atgctcatca aggattgtta cttgctcaac attgcagcct ttgtcggcaa
     1021 tggtgctcgc cacttctgcc agcgagcctg gtttgtgtat gatatttacg tttatgtcgg
     1081 tagaaagact atgttctaag cgctcgctcc actcaacacc aatccagcga ctggtatctt
     1141 taataaacga agatacatgt ctgcagtttc ttctatgcac taccaaccct cgttgtttgc
     1201 ttagatggcc aataacggta tcgccaggta tgggcgtgca acattttggg aacgatactg
     1261 caactccctc ggtgcctttg atgtataaag gattgtcttc tttcatggta tcgtcgcttt
     1321 cagactcacc cagtagaagt tgagccatca gtattgggtt tttgtcgcca gtgccaatgc
     1381 tttcataaag atctttgctg tctttgagcc caaggtcatt tagaaagttc ttaagttgtt
     1441 ttcttgaaat gttccgttgt tttttaccca atattgttaa agcgtgatta aacagccttt
     1501 ttcccaaggc aatggtttca gcaacgttta aattcttcag ataggctctt atgttcagcc
     1561 ttgcttttgc agtcgcgatg aaatttaacc agctgggatc ggggttggac tgtttactgg
     1621 ttaaaatctc gacggtctga ccactttgaa caatagttct aagaggcact tgttggcgat
     1681 caagtttagc accaatacaa ctgttgccaa catccgtgtg gacagcgtat gcaaaatcaa
     1741 cacaagtgga attgatcggg agcctaagaa tttttccttt aggtgtgaag acataaacct
     1801 tgtctgggta aagatcaacc ttgatacttt ccaaaaattc ttcaggatga gttgctccct
     1861 ggatttcttc aatggttgcc agccattctc ttgctctgat ctgtggagga acagcatctt
     1921 tctcttctgt tttgtatttc caatgtgctg caataccgct ttcagccacc ctgttcatgg
     1981 cctttgttct gatttgaacc tcaataggtg taccgtcggg ccccaacaaa ctagtgtgta
     2041 gtgattggta tccgttaatc cttggtatgg cgatgtagtc attaaactta tccataatcg
     2101 gtttgtatat ttgatgaact aatcccagta cctgataaca ctcctctact tgctctacaa
     2161 taatcctgat gccatacaca tcaataattt gatcaaaagg gagttttcta ttcttcattt
     2221 ttttatagat gctataaaga tctttttttc tgctttgaat ttcagcatca atacgagcct
     2281 cgtctaagtt ttttcgaaac cttgaagcga ttgttcgtag tatttttttc tggtggccgg
     2341 ttctgcgttt taatgcgtta ccaagaattc tttgcctaaa agggtaggca tgcttgaagg
     2401 cgtctacttc cagagcggtt ttcatatgat aaatacctaa acggtttgcg atcggggcat
     2461 aaatatccag tgtttccctt gcgatttttt gttgtttgtt tttatccaag gcatcaaggg
     2521 tttgaatatt atgcagccta tctgagagtt taattaaaat gacgcgtatg tcttcaatca
     2581 tggcaagcat catttttcta aaactttctg cttgtgtgct ttcattctct gagtactcta
     2641 attgatctaa cttactcaca ccatcaacta aatttagtac attttgacca aactccttaa
     2701 caataaggtc agggttgatc gtgctgtctt ccaagacatc atgcagcaag ccagcggcaa
     2761 tggattcttt atctaaacga agatctgcca aggttttggc taccgcaata gggtggttga
     2821 tgtagggctc gccagatctt ctcagttgct cttcgtgagc aatgtttgca aacacgtaag
     2881 cttttttcac aaactcctga tcactctgtc ccagataatg caaattatcg tggagctgcc
     2941 tcattaattt aggcagtttt ttgctcatca ttgctgctgc tttatctgca aactcttcca
     3001 taacacacta agatcattta tctattgact attctacgca atgagaggga tgaaaggtat
     3061 ggatctatca gatggaagca attatcagtg aataaaatca gaccggctgc attattgcag
     3121 ctgtttaatt ttttataaaa gaaataaaaa agaaactatt cttcgttcaa tgaccttgct
     3181 aactcttcag caatttcttt ttctagtgcg ttctcagagg tatgaaattc aggattgtcc
     3241 attaagcctt ctgaaatttc tctcaatgct aaaacaacgg gcttgtcgtt ttcatcatct
     3301 tcaagcaatg tatcagctcc gtcggctatc cttcttgctc ttttggaagc aagtttgacc
     3361 aaatcaaaaa cactatcaac gtgctcaaga caatcttcta ctgtaactct agccataata
     3421 agtaagaaaa attaatacgg gagacaagac tacccagatc cgcggatatg tcaattagtt
     3481 tctatttgag gtccaataaa gctttcaatg atagtcagag cctcgttgct gatgttgtgt
     3541 tttatgtttt gagaggagtc aagtaaaccc ataatttcta ggcaggttga gtcaatgtct
     3601 ttattgataa tgacacaatc aaaatgaata tattgctcaa tatcctgaac agctaattta
     3661 taccgatgta caatttgatc ggctgagtcg gtggctctgt tggtcagtct tttttttaat
     3721 tcttgcttgg agggagggac gataaaaata gacatacaat ctggattggt tttacgaata
     3781 gatgctgcac cctgccagtc aatttcaaga aaaactgttt ggcctttttt ttgtgcttgt
     3841 gagatttctt tttttggagt gccgtaataa tgatcgaata cttttgcatg ctcaacaaac
     3901 agattcttat tgatcatttc tgtaaatagt tgttgatcta taaaaacata atcaacaccg
     3961 tctgtttcgt tggctctttt tgctctggtt gtataagagg tggaggctac aaaattggct
     4021 cgttgagaaa taagtttttt cacaatagtt gttttacccg ttccagaagg agcagagaca
     4081 accaccagat ttatgttatt gtttgccaaa ataacctgtt aaaactatat aatcactcac
     4141 tgtaacaaac ctaatttttt taatcttaat cttgtgcgta taaaaaccat agtgttggca
     4201 tcaaacaatg ccaataaagc caaagaaata caaatgctgc tgggtgatca ggttgaaatt
     4261 attatgcaac aagatttgaa catagagtcc atacccgaaa caggtaaaac ctttgaagaa
     4321 aatgcctgct taaaagcaac aacggcctac aagaaagcca aactacctgt gattggagat
     4381 gattctggct tagaggtaga ttgcctaaac ggccagcctg gaattcattc tgctcggttt
     4441 gctggagacg gcgcttcaga taaagacaac atcactaaat tattagacca gatgacaggc
     4501 gttccgcttg aacaaagaac cgcacggttt aaatgtgtca ttgctttgat tggcctggaa
     4561 gagcttgata aagtcaattt ttttcatggt gagtggaatg gttttattgc tgagcaggta
     4621 accggacata atggttttgg ttatgatcct gtgtttgttg atccaggcac ccaattaaca
     4681 gcggctcaac ttgatcaggg tttaaaaaac aaattaagcc atagagggca agccatagat
     4741 gctgtaaaga attacttgag tttttaattg cacaacgctg ctacacatcc cacgtcatta
     4801 tatgtccact ttccgtggtg ccaatctaaa tgcccctatt gtgattttaa ttcatacaaa
     4861 ttaaacaaaa gactacctgc tagtgaatat gtatctacgc tgattgttga tttaaaaaaa
     4921 acccttacat tttctaatag ctcactcgag cttaaatcta tatattttgg cggtggcact
     4981 cctaatctat ttccgccaga tgcgtttaaa actttattag acaacatcca cgaagaatgt
     5041 aattttactg atgatatcga aatcactttg gagatgaacc ctggcatcgg ccaacctgat
     5101 aatctggaaa gatatttagc tttgggtatt aatcgcttgt ctgttggcgc acagacattt
     5161 tcaaaaaaaa tactcaacaa tattggcaga ctgcataacg agaaccaaac aatccaattg
     5221 tataagcagg ctaaaaagac agggtttgag aacattaatt tagatctgat gtttggtttg
     5281 ccaggacaaa ccagtagtca gtttgaccat gacctcaggc aattagttga cttaagtcca
     5341 gagcatattt cattttatga gttaaccata gaggcgggga cccagtatta ccttaaaaaa
     5401 cctgatctcc ctgatcatga aataatatgg agcatgcatg accagggaaa aaatgtattt
     5461 gaacaagcag gctatcaaca atacgagatt tctgcgtttt ctaagccagg ttttcagtgt
     5521 gtacacaatc tcaattactg gttgtatggt gattttgtgg ctattggcgc aggggctcac
     5581 gggaaatata cagggccaac aagggacatc ttcaggtacg tgaaaaaa
//
LOCUS       DASCTH010000122         1891 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000170758, whole genome shotgun sequence.
ACCESSION   DASCTH010000122 DASCTH010000000
VERSION     DASCTH010000122.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000170758"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="QGH35_02565"
     CDS             complement(<1..1601)
                     /locus_tag="QGH35_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013069.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HJL81450.1"
                     /translation="MMDSNYNQPNQSKINQEVVDSNKGLINFFVSHKVAGNLLMALLI
                     LFGVYGLSQLKRQIMPDFGLEIVNINIEWMGASAEDIEANIIEAIEPEIRFINQVDKV
                     DATAYEGKGIISIHFAQDTSMSSALSDVQSAVARITTFPQDMEKPIISEIVQKDEVCR
                     IDISGPFSEKTLKYFARQMRDDLINMGLANVEFEGDRDPEIWIEVPANNLKELKLSIE
                     DISTRVSASSIDLPSGSTNSGGISTLIRSEQLAKTPQELRNIEVVSMETGEKLYLKDI
                     ANIFETYQENSDYQINSEGRSIGLTVFRTRGADSLVSHEIVEEYMDELLLHYPQSLKV
                     VVFDVFANAVRERLFMLLKNGALGLALVLLVLFIFLSGRVAFWVAIGMPAAYLAALGA
                     MSVLGMSLDMVSMFALTMGIGIVVDDAIVVSEHTTTLHRRGIPYLDAARRAAQTMFAP
                     VLAASLTTIAAFLPVLMLGAEVGNIIAPIPIALSLIILGSLIECFLIMPSHLKTSLKR
                     MERLDKKEPRKFDVVFNHFRDHYVIP"
     gene            complement(1601..>1891)
                     /locus_tag="QGH35_02570"
     CDS             complement(1601..>1891)
                     /locus_tag="QGH35_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81451.1"
                     /translation="DESEFSLIRPGAFVRLSVTDKTYNQVISVPNTAVYDDEYIFIIL
                     NDRLVKRLVVVMGYDGTNTLIKADSTNEIKVGDLIMVNQLREAGEGVKVDIL"
BASE COUNT          567 a          415 c          343 g          566 t
ORIGIN      
        1 ggaatgacat agtgatccct aaaatgattg aatacaacat caaactttct gggttctttt
       61 ttgtctaatc gttccattct ttttaacgat gtttttaaat gtgatggcat gattaaaaag
      121 cattcaataa gacttcccaa aataatcaag gacaatgcta ttggaatggg tgcaataata
      181 tttccaactt cggccccgag cattaaaacg ggtaaaaaag cagcaatagt ggtaagagat
      241 gctgctaaga caggagcaaa catagtctgt gccgctcgcc tggcagcatc taagtaaggg
      301 atacctctac gatgcaaagt ggttgtatgc tcagaaacta caatggcatc gtcgacaaca
      361 ataccaatac ccatcgttaa tgcgaacatg ctgaccatat ccagagacat accaagtact
      421 gacatggctc ctagtgctgc aagataagct gccggcatgc caatggcaac ccaaaaagct
      481 acccgaccac tgagaaaaat gaataatacc aataacacaa gtgccaaacc cagggctcca
      541 ttcttaagca gcataaaaag gcgctccctg acagcattcg caaacacatc gaaaaccacg
      601 acctttagac tttgtgggta atgcaataac agttcatcca tgtattcttc gacaatttca
      661 tgcgaaacca gagaatccgc accccgtgta cgaaaaactg tcaagccaat ggatcggcct
      721 tcgctgttga tctgataatc agagttttct tggtatgttt caaatatatt ggcaatatcc
      781 ttcaggtaca atttttcacc ggtttccata gacaccactt caatgttcct cagttcctgt
      841 ggggttttgg caagttgttc gcttctgatt agagtcgaga ttcctcctga gttggtgctt
      901 cctgatggca agtcgatact tgatgctgaa acacgggtag aaatatcttc tatagatagc
      961 ttcaattctt tgagattatt agcagggact tcaatccata tttctggatc cctatcacct
     1021 tcaaattcta catttgcaag gcccatatta atcaaatcat ctcgcatctg tctggcaaaa
     1081 tatttcagtg ttttttccga gaatggaccc gatatatcaa tgcgacagac ttcatctttc
     1141 tggacgattt cagaaataat cggtttttcc atatcctggg gaaatgttgt gatcctggca
     1201 acggctgatt gcacatcgga taacgctgat gacatggatg tatcctgcgc aaaatggatt
     1261 gatatgattc ctttgccttc atatgcggtt gcatctactt tatcgacttg attaataaat
     1321 cgaatctccg gttctattgc ttcgataata ttggcttcaa tatcctctgc actggctccc
     1381 atccattcaa tattaatgtt tacgatttcc aatccaaaat cgggcattat ctggcgtttt
     1441 aattggctta aaccatagac tccaaagaga attaacaatg ccattaatag atttccagca
     1501 accttatgac tgacaaaaaa attgatcaag cccttgttgg agtctacaac ttcctgattg
     1561 atctttgatt gatttggctg gttgtaatta ctatccatca ttataaaata tcgaccttca
     1621 ctccttctcc tgcctctcga agttgattca ccatgatcag atcaccaact ttgatctcgt
     1681 tggtcgaatc agctttaata agtgtgttgg ttccgtcata ccccatcact actaccaatc
     1741 gttttactaa gcgatcattg agaataataa aaatatactc atcatcataa acggcagtat
     1801 ttggaacaga aataacttga ttgtaagtct tgtcggtaac tgacaatcta acaaaggctc
     1861 ctggtcttat tagagaaaat tctgattcat c
//
LOCUS       DASCTH010000123         2364 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000171136, whole genome shotgun sequence.
ACCESSION   DASCTH010000123 DASCTH010000000
VERSION     DASCTH010000123.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000171136"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            41..1123
                     /gene="prfA"
                     /locus_tag="QGH35_02575"
     CDS             41..1123
                     /gene="prfA"
                     /locus_tag="QGH35_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HJL81452.1"
                     /translation="MKESVINKLKQIVEQYRSIEKQLSDEETQKNNTLMIKLSKEYSR
                     LGPLVDLFGRYQLLIEERNDASEMIGEKDDELKTLIQQELQNIDQQLLDLEEQINPLL
                     LLADPRDQNNIFLEIRAGTGGQEAAIFVGDVFRMYSRYCEQREWSVDILNQNESGQGG
                     YKEIIAKIIGKGAYSQLKYESGTHRVQRVPKTESQGRVHTSACTVAVIAELDDLDEIE
                     LNPSDIRVDTYRASGAGGQHVNKTDSAVRLAHIPSGIVVECQDNRSQHKNKAAAMTLL
                     KAKIFDMEQEKLSQQQAKERKMLIGSGDRSERIRTYNFPQGRITDHRINLTLYNLAQV
                     MEGELDTLIQSLIQENAAKQMASVET"
     gene            1130..1996
                     /gene="prmC"
                     /locus_tag="QGH35_02580"
     CDS             1130..1996
                     /gene="prmC"
                     /locus_tag="QGH35_02580"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HJL81453.1"
                     /translation="MTINNISSSMLLEKGETALKQTSSTPRLDSEILLANILKMERIP
                     FYSKEIFPNRKEVQAYNKDIKKRAKGMPIAYLIGSKEFWSLNMQVNKHTLIPRPETEH
                     LVELALSHLVDLKKDYNILELGTGCGAIAIALAHERPHAKIYATDISVEAIRSARDNA
                     KCLTVENIQFICSNWFEAIRATKFDLIVSNPPYINEKNINDYDSLSLSYEPKIALFAD
                     NHGLNCVNTIIGNSIKYLEKDGWLMIEHGYDQAHHCRQFMKSVLLQNIHTNNDLSGKQ
                     RVTEGQKTKLTL"
     gene            1993..>2364
                     /locus_tag="QGH35_02585"
     CDS             1993..>2364
                     /locus_tag="QGH35_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013094.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HJL81454.1"
                     /translation="MTPLQQEDLVRYSRHLSLDNFTNSSQLKINQSSILIVGIGGLGT
                     ITALYLANSGVGNIILNDFDSVDLSNLPRQILFNKEDINKNKADVAVEKLRAFNTAIT
                     LRSLNNKLSETELSKVISNVDI"
BASE COUNT          883 a          386 c          426 g          669 t
ORIGIN      
        1 attccatacc ttttgaattt tttttatttt tggaaacaca atgaaagagt ctgtcatcaa
       61 taagttaaag caaatagtag aacaatatcg ttctattgaa aaacaattat cagatgaaga
      121 gacacaaaaa aacaatacat taatgataaa actgtccaag gaatattctc gtctcggtcc
      181 tttggttgat ttgtttggca gatatcaact cctcattgag gaaagaaatg atgcatctga
      241 gatgattgga gagaaagatg atgaattaaa aacattaatt caacaggaac tacaaaatat
      301 tgatcaacaa ctattagatt tagaggaaca aattaaccca ttgctactat tggcagatcc
      361 aagagaccag aataatattt tcttggaaat aagagcagga acaggaggtc aagaagctgc
      421 tatttttgtt ggtgatgttt ttagaatgta ttccagatac tgtgaacaaa gagagtggtc
      481 tgttgatatt ctaaatcaaa atgaaagtgg tcaaggtggt tataaagaaa ttattgcaaa
      541 aattattgga aaaggtgctt attctcaact taaatatgaa tcaggaacac accgggttca
      601 gcgggttccc aaaaccgaat cacaaggtag agttcacaca tccgcttgta ctgtagctgt
      661 catagcagaa ttagatgatt tggatgaaat tgaactgaat cctagtgata ttagagtcga
      721 tacgtataga gcatctgggg caggaggcca gcatgttaat aaaactgatt ctgcagtccg
      781 tttggctcac attccaagtg gtattgttgt tgaatgccaa gataatcgat cacaacacaa
      841 aaataaagca gcagctatga ctctacttaa agcaaaaata tttgatatgg aacaagaaaa
      901 attatctcaa caacaagcaa aagaaagaaa aatgttaatt ggcagtggcg atcgatctga
      961 gagaatcaga acgtataact ttccacaagg taggattacc gatcacagaa taaatttaac
     1021 gctctataat cttgcacagg tcatggaagg tgaacttgat actctcattc aatcattgat
     1081 ccaagaaaat gctgccaaac aaatggcatc agtggaaact taacttgtaa tgacaataaa
     1141 taatatttca agctccatgt tgctcgaaaa aggtgaaaca gctctaaaac agactagttc
     1201 aacacctcgc ttagattcag aaatactttt ggcaaatatt ttgaaaatgg aaagaattcc
     1261 tttctattcc aaagaaattt ttcctaacag aaaagaggtc caagcatata acaaagatat
     1321 aaagaagaga gccaaaggta tgcccatagc ctatttgata ggtagtaaag aattctggtc
     1381 tctcaacatg caagtcaaca agcatacttt aatcccgaga ccagaaaccg agcatcttgt
     1441 tgaattggca ttgtcacact tggttgattt aaaaaaggat tacaacattc tcgaacttgg
     1501 aaccgggtgt ggtgcaattg ccattgccct ggctcatgaa agaccccatg caaagattta
     1561 tgccacagat atttcagtag aagcaataag atctgctaga gacaatgcaa aatgcttaac
     1621 tgtagaaaat atccaattta tatgcagcaa ttggttcgag gccattagag cgactaaatt
     1681 cgacttaatc gtctcaaatc caccttatat taatgagaaa aacattaatg actatgactc
     1741 cctttcatta tcatatgagc caaaaatagc attatttgca gataaccatg ggttaaattg
     1801 tgtgaataca atcattggca atagtattaa atatctggaa aaggatggct ggctaatgat
     1861 tgaacatggc tatgatcagg ctcatcattg tagacaattc atgaaatcgg tcttgttgca
     1921 aaatattcat acaaacaatg acttatctgg aaaacaaaga gtgacagaag gtcaaaaaac
     1981 aaagttaaca ttatgactcc attacaacag gaagatcttg tcagatatag cagacattta
     2041 agtcttgata actttacgaa ctcatcgcaa ctaaaaataa atcaatcatc tattctaatt
     2101 gttggaatag gtggtcttgg aactatcact gctctctatt tagcaaatag tggtgtaggc
     2161 aatattattc tcaatgattt tgattctgtt gatctttcaa atttaccaag acagattctg
     2221 tttaataaag aagatattaa taaaaataaa gcagatgttg cagttgaaaa attaagagca
     2281 tttaatactg caattacact acgttcactc aataacaaac tcagtgaaac tgaattatca
     2341 aaagtaatat caaatgtcga tatt
//
LOCUS       DASCTH010000124         2489 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000172783, whole genome shotgun sequence.
ACCESSION   DASCTH010000124 DASCTH010000000
VERSION     DASCTH010000124.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000172783"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(147..1211)
                     /gene="tsaD"
                     /locus_tag="QGH35_02590"
     CDS             complement(147..1211)
                     /gene="tsaD"
                     /locus_tag="QGH35_02590"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HJL81455.1"
                     /translation="MLKSKKNLCILGIETSCDETAVALMDTMKGLVAHQVFSQVSLHN
                     KYGGVVPELASRDHIKKLLPLIDDLIKSQDFKLENLDAIAYTAGPGLAGALLVGSVIA
                     NGLAFSLEIPAIPVHHMEAHLLAPLIEGRHSEMPFIALLVSGGHTLLVQVNERGSYTL
                     LGETLDDAAGEAFDKGAKLLGLGYPGGPAIAELAQSGDSGVYPFPRLMMKEKHNLNFS
                     FSGLKTALMYKVKDLTNKELLTDKTKADLAVAYEKAIVDSLVQKTELAVNLTGIQSVV
                     IAGGVGANKKLRNSMRQKLAEKDVNVSYPSVEFCTDNAAMIALTGLFNYQKGMHNIDY
                     KIEVNPRWPIDKIESTISRH"
     gene            1335..1550
                     /gene="rpsU"
                     /locus_tag="QGH35_02595"
     CDS             1335..1550
                     /gene="rpsU"
                     /locus_tag="QGH35_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HJL81456.1"
                     /translation="MPIVTVKDNEYFDYALRRFKRSCEKAGIINDYRKREFYEKPTQA
                     RKRKKAAAVKREKKRTYRERNRARRLY"
     gene            1563..2009
                     /locus_tag="QGH35_02600"
     CDS             1563..2009
                     /locus_tag="QGH35_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020977.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HJL81457.1"
                     /translation="MPLLNQIQEDVKSALRNQERLKLTTLRMLVAAIQQKEIDTRETI
                     TEDGILAIIEKQVQLRKEAAEQYQAADRLELFEKENEEANILKLYLPEKLDDQAMVTI
                     IENTIASVGAKTIQDMGQVMSALKNQSQGRIDMKLASTIVRNLLVK"
     gene            2109..>2489
                     /locus_tag="QGH35_02605"
     CDS             2109..>2489
                     /locus_tag="QGH35_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020032492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HJL81458.1"
                     /translation="MSDLIPQDFIDDLVQRIDIIEVISNRIEIKKAGKEYKGLCPFHT
                     EKTPSFTVSQEKGFYHCFGCGAHGTALGFLMDYENHTFIEVIEELSKIAGVEIPKTKQ
                     DRAKNKKNKSLQELTAEIMGQFIQN"
BASE COUNT          842 a          481 c          456 g          710 t
ORIGIN      
        1 ctttatagaa tcagtctctg aggctttatt aacatccgtt tccatttcaa gatcaattga
       61 aattatttga gggttccttt tttcccactc ccaaatacca atgacagcct ttattttaag
      121 atcggttaag ataattttat ccatcattaa tgccgtgaaa ttgttgattc aatcttatcg
      181 attggccaac gcgggtttac ctctattttg taatcgatat tgtgcatgcc tttctggtaa
      241 ttaaataagc ctgtcaaagc aatcatggca gcattatcag tgcaaaactc tacgctagga
      301 taggacacat tgacatcttt ttctgccaac ttttgcctca tactattgcg taacttttta
      361 tttgccccaa ctccacccgc tataacaacg gattgaatac ctgtaaggtt tacagctaat
      421 tctgttttct ggaccaggct atccacaata gctttttcat aggcaacagc aagatcagct
      481 tttgttttgt cggtaagaag ctccttgttt gttaagtcct taaccttata cattaaggca
      541 gtttttaagc cgctaaagct aaaatttaga ttgtgtttct ctttcatcat cagtctcgga
      601 aaaggataga ctcctgaatc accggattga gccagttctg ctatcgcagg acctcctgga
      661 taaccaagac ctagcaattt tgccccttta tcgaaagcct cacctgcggc atcatctaga
      721 gtttcaccta aaagtgtgta agagcctctt tcattgacct gtaccaacaa tgtatgcccg
      781 ccagaaacca acaaagcgat aaatggcatc tcagaatgtc taccctcaat cagaggagcc
      841 aataaatgag cttccatatg atggacgggg atagcaggga tctccaacga gaaagccagc
      901 ccattagcta tcacggaacc tacaagtaag gcgccggcaa gacctggtcc tgctgtataa
      961 gcgatagcat ctaaattttc taatttgaaa tcttgagact ttataaggtc atcgatgaga
     1021 ggcaatagtt tcttaatatg gtctctggaa gccagttctg gcacaacacc tccatatttg
     1081 ttatgcagag aaacttggct aaacacctga tgagcaacga gccctttcat ggtatccatg
     1141 agggccacag ctgtttcatc acaactggtt tcaatgccca aaatacacag atttttctta
     1201 gatttaagca tggttaacag tctttgttat tacactaaat ttctttgcat tttagtgaac
     1261 taaacggtat atttcagcgt tcgtttaagg gattaattat atattgaatg tattgacata
     1321 aaaggaatag tttcatgcca attgttacag taaaagataa tgagtatttt gactacgcac
     1381 tcagaagatt caagcgatct tgtgaaaagg caggcattat caatgattat cgaaaacgag
     1441 aattctatga aaagccaacg caggcaagaa aaagaaaaaa ggctgcagcg gttaaaagag
     1501 aaaaaaagag aacttatcgc gagcgcaatc gagcccgtcg cctgtattga acaacatcat
     1561 aattgccatt acttaatcaa attcaagaag atgtaaagag tgcgcttcga aatcaggagc
     1621 ggctaaaact cacaacatta agaatgctag tggctgctat ccaacaaaaa gagatcgata
     1681 caagagagac aattaccgaa gatggaatac tggcgattat tgaaaaacaa gttcaattac
     1741 gaaaggaagc agcagaacaa tatcaagcag cagatcggtt agagcttttt gaaaaagaaa
     1801 atgaagaagc caatatctta aaattatatt tacctgagaa gcttgatgat caagcaatgg
     1861 ttacaataat tgaaaacacc attgcgagtg ttggtgccaa aacaatacag gatatgggac
     1921 aagtaatgtc agcactaaag aatcaatcac aaggaagaat tgatatgaaa ctagccagta
     1981 ccatagtcag aaatcttctt gtaaaataat caacaattaa ccctctcaag taatttttac
     2041 tttttatctc tttctgcata gactaattct ctacgtaaaa atagagaaca tttaattatc
     2101 ctttttatat gtcagatctt atcccacaag attttataga tgaccttgtc caaaggattg
     2161 acatcattga agtgattagt aatcgaatcg aaataaagaa agcaggcaaa gaatataaag
     2221 gactatgccc ttttcatact gaaaaaacac catcattcac agtcagccag gagaaaggtt
     2281 tttaccattg ctttggctgt ggtgctcatg gtactgcact aggtttcttg atggattatg
     2341 aaaatcatac gtttattgag gtcatagaag aactctcaaa aatagcaggc gtagagattc
     2401 caaaaactaa acaagataga gccaagaata aaaaaaataa aagcttacaa gaattgactg
     2461 ctgaaattat gggccagttt attcaaaac
//
LOCUS       DASCTH010000125         1758 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000173250, whole genome shotgun sequence.
ACCESSION   DASCTH010000125 DASCTH010000000
VERSION     DASCTH010000125.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000173250"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(371..>1758)
                     /locus_tag="QGH35_02610"
     CDS             complement(371..>1758)
                     /locus_tag="QGH35_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016289.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HJL81459.1"
                     /translation="LIEGLLGDTPIEEDLKELCDVIGGRPTGSRANSLSIDWGLKKFQ
                     EAGVSASKEAFTMPGLWLENESTAQIRGAAEFEPRVVALTFSKGTHRTGLRGKLVDGG
                     HGTIDDFARLGENARGNFVIIETDELVDIDGLFKEYADAVLIEDLAYEAGVKGLVYMS
                     SRPMRLLYRHNASRSFENTLPMVVMAREDAKRCLRILRNDESLALLVKINVRKGGAYK
                     SYNVIGEIKGSERPEEVVIIGAHLDSWGLGTGANDNGCNIAMMIDIARQMIKLDLKPK
                     RTIRFALWNGEELGLYGSMAYTEQYESQLDNHVMAMSVDIGSGPITGFFTGGRPDVLN
                     VTNKIMDPLTDLGMFTNIDIPLVGTDNFDFMMHGVGNLIANHEPANYAPNYHAESDTY
                     DKVNLRALKHNSAIVAAVTLGYANDLNIDLPRQSKEEIDKLVESTDLEQQMKSMMGIW
                     YQWIDGKRGRK"
BASE COUNT          495 a          402 c          285 g          576 t
ORIGIN      
        1 tactctaata tttgtttttt tatcatcact attacactct gaaatattat gttgttcaca
       61 ttcaaattca gtgctatcgc atgtcaatga aaaaattgtt acaaataata cgaagaaaat
      121 atacttgact ctattggact tcattctata tccttatttt gtttttatat gagtaatgta
      181 tctgatatta cacttttatg gggggcaacg ttggtatatt ttaaattata gtacaaacaa
      241 aaagcccccg ttaggagact atttgagcca gaggcgtttc atttactgcc catctttttc
      301 atgtgaaatg agcccttttt ttgattaaga attatatatt aatattagct acccattgat
      361 aaagacgatc ttattttcta cccctttttc catctatcca ttggtaccat attcccatca
      421 tagatttcat ctgttgctca aggtcagtag attcaactag tttatcaatt tcttcctttg
      481 actgccgcgg aagatcaata ttgaggtcgt tggcatatcc aagtgtgacc gctgctacga
      541 tggccgaatt gtgctttaat gcccgcaagt tgactttatc gtatgtatcc gattcagcat
      601 gataattagg cgcataattt gcgggctcat gatttgcaat tagattccct accccatgca
      661 tcatgaaatc aaaattatct gtccctacaa gaggaatatc aatatttgta aacatgccta
      721 gatctgttaa aggatccatg atcttgtttg taacatttaa gacatctggg cgaccaccag
      781 taaaaaaccc ggtaatcggg ccactaccaa tatctactga cattgccatg acatgattat
      841 caagctgtga ttcatattgc tcggtatagg ccattgatcc atacagtcca agttcttcgc
      901 cattccaaag ggcaaatctg atcgttcgtt taggctttaa gtccagtttt atcatttggc
      961 gtgctatatc aatcatcatt gcgatattgc agccattatc gtttgcgccc gttccaagtc
     1021 cccaagaatc caaatgtgca ccaattatga ctacttcttc agggcgttca gaacccttga
     1081 tttccccaat aacattataa gatttataag caccaccctt tctcacgttt atttttacca
     1141 aaagagccaa agattcatcg ttacgcaaaa ttctcaaaca tcttttagcg tcttctctgg
     1201 ccatgactac cattggtaaa gtattctcga agctccttga tgcgttgtgc cgataaagca
     1261 gccgcatggg gcgagaagac atataaacca aaccttttac accggcctca tatgccaaat
     1321 cttcaatcag tactgcatca gcatattctt taaaaagacc atctatatca accagctcat
     1381 ccgtttcaat aatgacgaag ttccctcttg cattttcacc cagtcttgca aaatcatcga
     1441 ttgttccgtg cccaccatct acaagttttc ctcttaaccc agtacggtga gttccttttg
     1501 aaaaggtaag ggcaacaact ctaggttcaa attctgcagc acctctaatt tgggcagtgg
     1561 attcattttc aagccatagt cccggcatcg tgaaggcctc tttgctggca cttacaccag
     1621 cctcttggaa tttttttaat ccccagtcta tggaaagaga attggctctt gacccagttg
     1681 gccgaccccc gataacatca caaagttctt ttaaatcttc ttctattgga gtatcaccca
     1741 acaatccttc tatcaatt
//
LOCUS       DASCTH010000126        15795 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000173543, whole genome shotgun sequence.
ACCESSION   DASCTH010000126 DASCTH010000000
VERSION     DASCTH010000126.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15795)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15795)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15795
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000173543"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /gene="rpsK"
                     /locus_tag="QGH35_02615"
     CDS             <1..300
                     /gene="rpsK"
                     /locus_tag="QGH35_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HJL81460.1"
                     /translation="IVTITDRAGNALSWATAGGSGFRGSRKSTPFAAQVAAEKAGKAA
                     QEFGLKNLDVKVKGPGPGRDSALRALNAIGFSINSIEDVTPIPHNGCRPPKQRRV"
     gene            313..936
                     /gene="rpsD"
                     /locus_tag="QGH35_02620"
     CDS             313..936
                     /gene="rpsD"
                     /locus_tag="QGH35_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HJL81461.1"
                     /translation="MARYLGPKCKLSRREGTDLFLKSGIKPIENKCKLNTVPGSMVGS
                     RRDRLSDYGLQLREKQKLRRMYGVLEKQFKNYYKKAAKIKGSTGENLLKLLECRLDNM
                     VYRMGFAITRAEARQLVSHKAILVNNSVVNIPSYQVSEDDEISISEKAKGQFRIKNAL
                     DISGQLGICDWLSVDEKKFSGTVKSIPDREDVLPDINENLVVEFYSK"
     gene            954..1988
                     /gene="rpoA"
                     /locus_tag="QGH35_02625"
     CDS             954..1988
                     /gene="rpoA"
                     /locus_tag="QGH35_02625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HJL81462.1"
                     /translation="MEEYGEVEVQEIEKEEIVLDLLNPRVIKLESQHELQSRVSIEPF
                     ERGFGYTLGNALRRVLLSSLPGYAITEVQIDGVLHEYTTIDGVQEDVVEILLNLKNVA
                     INLNARDEAEFVISKKGPAVITAGDIQVDHDALVVNPEHHIATISSKKEINMQLKVQK
                     GTGYRPVTIDAKQDDDNIVGLLKLDASFGPIKKVSYRVESARVENRTDLDKLIVDLET
                     DGTIEPESAIKYAGAILQNQLSIFVDLTRVQEQQKAEEEVLIDPIMLRPVDELELTVR
                     SANCLKAENANYIGDLVQKKEVELLRTPNLGKKSLNEIKEVLASHGLSLGMELEIWPP
                     KELVTDEDNQ"
     gene            2027..2416
                     /gene="rplQ"
                     /locus_tag="QGH35_02630"
     CDS             2027..2416
                     /gene="rplQ"
                     /locus_tag="QGH35_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HJL81463.1"
                     /translation="MRHKKTGRRLGRKSDHRKAMFRNMAVSLIVHESISTTLPKAKEL
                     RRTIEPLITLAKQDGVSKRRRAFDKLRDKAAVGKLFDEIGPRFKERTGGYLRILKKGM
                     RSGDSAQMALVQLTDYEIEFSDSLTDD"
     gene            2579..3580
                     /gene="trpS"
                     /locus_tag="QGH35_02635"
     CDS             2579..3580
                     /gene="trpS"
                     /locus_tag="QGH35_02635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013819783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HJL81464.1"
                     /translation="MIQKTILTGITTTGTPHLGNYAGVIRQAIDASFKDEHRSFYFLA
                     DYHALIKNYDPIKIKQSCLEVAATWLALGLDFDRTFFYRQSDIPEIPELMWILNTFTA
                     KGLMNRAHAYKSAVQANKESDTKDPDKGISVALYSYPVLMAADILMFKSTHVPVGQDQ
                     KQHLEMTRDIAQRFNHNYGDIFVIPEVVIERDASVLPGLDGRKMSKSYNNSIPLFLPS
                     GELRKLIMKIKTNSLEPGEPKTTKNCPLFDIFQGVATPEATSKFKNKLEQGLAWGEAK
                     EELFLYIEDLFSGPREEYFKLMNDPKHVDKLLKSGAAKARELSQPFLREIRAAIGID"
     gene            complement(3593..6409)
                     /gene="uvrA"
                     /locus_tag="QGH35_02640"
     CDS             complement(3593..6409)
                     /gene="uvrA"
                     /locus_tag="QGH35_02640"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010324078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HJL81465.1"
                     /translation="MQHIRIKGAKTHNLKNLDLELPRNKIIILTGLSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLSMMEKPDVDLIEGLSPAISIEQKTTSHNPRSTVGTVT
                     EIYDYLRLLYARTGIPKCPEHGIELGIQNISEMVDHVLQIKEGSKLVLLSPIVNDRKG
                     EHVELIKTIKAKGFIRVRINQKIYDIDDLPTIDSKKKNTIEVVVDRFTVKPDIALRLT
                     ESFETALEMADGVAKIVNQEKDHPMLLFSDRFACPVCGYSIQELEPRSFSFNNPAGAC
                     QSCDGLGVEQFFSPHLVIKNPKLSISEGAIRGWDRRNIYYFYLIKSLSKHFGFNIDTP
                     YESYNETVQNTLLYGSGDEKIQFYYPGRRGRKISRKHVFEGVIPNLIRRYKETESKAV
                     KDALSKFMSVKSCLNCHGTRLNEAARHVFIKNKTIADITKMNIDEALTFFQELNIKGT
                     RGEIASNIIKEIQERLLFLSNVGLNYLNLERSAETLSGGESQRIRLASQIGSGLVGVM
                     YILDEPTIGLHQRDNERLIATLTKLRDLGNTVIVVEHDEAAIRSGDYIVDLGPGAGNA
                     GGEVVAQGNLECLLKEPKSLTGQYLKKEKSIPMPSKRLAPAKNKIIKIKNAQKNNLKA
                     LNVDIPLNLFTCVTGVSGSGKSTLINGTLQPLIASTLEKKPCNHLSKTFIEEPVNIHK
                     MIDISQGPIGRTPRSNPATYTGLFTPVRELFSSTQESRSRGYKPGRFSFNVKGGRCET
                     CQGGGVIKVEMNFLADIYVTCDACKGKRFNRETLEIMYKNKNIFDVLEMSVSDAYRFF
                     SNIPRIKNKLATLDEVGLGYIKLGQSAVTLSGGEAQRIKLSKELSKRGNSGTLYILDE
                     PTTGLHFHDIKQLLKVLIKLRNEGNTVVVIEHNLDVIKTADWIIDLGPEGGDHGGEII
                     VEGSPETVAKNKLSYTGQYLKQVL"
     gene            6615..7046
                     /gene="ssb"
                     /locus_tag="QGH35_02645"
     CDS             6615..7046
                     /gene="ssb"
                     /locus_tag="QGH35_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015444870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HJL81466.1"
                     /translation="MARGINKVIVLGNLGADPDARLMPNGNAVTNISVATSESWIDKE
                     SGDRQERTEWHRVVFFGRLAEIASEYLKKGSQVYIEGKLRTRKWEDKEGNERWTTEII
                     ANEMQMLGERMTSSASTDNFETPKQSANNASSDDFDDDIPF"
     gene            7074..8393
                     /gene="miaB"
                     /locus_tag="QGH35_02650"
     CDS             7074..8393
                     /gene="miaB"
                     /locus_tag="QGH35_02650"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HJL81467.1"
                     /translation="MSGKIFIKSFGCQMNEYDASKMADIMCHDQDMKLTDDPSKADLL
                     LLNTCSVREKPQEKVFSQLGRWRAYKKNNPNIIIGVGGCVATQEGESIIKRAPYVDMV
                     FGPQTLHRLPKMYDEVIASRHSVVDISFPKLEKFNFFPKTNQIKPSAFVSIMEGCSKL
                     CSFCVVPYTRGLEVCRFPEQVMEEIQGLASEGVREVNLLGQNVNAYRTKDIQGNKIKL
                     SNLIEQVSTIIGIDRIRFTTSHPIQFSEDLIQSFANPKVANFLHLPVQSGSNKILQLM
                     QRKHSVEAYIDKIERLRDLRPDISVSSDFIVGFPGETEDDFEQTLTLIDTIGFDQSFS
                     FIFSPRPNTSAASMEDTIGHDIKLARLQTLQAKITEHAMAISRKMIGAKERVLVEKQS
                     KKIKTQMTGRTENNRWVNFDGHQDLIGQFVDLIITEALPNSLRGRLT"
     gene            8421..9365
                     /locus_tag="QGH35_02655"
     CDS             8421..9365
                     /locus_tag="QGH35_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HJL81468.1"
                     /translation="MNHKEFEIDSTDNNNLVNLLGVFDKNLRVIEDFLSIEIKNRGNI
                     FLLSGQREDLIKGEEIIRHLYKAASNENLSERKIHLLLRKYMGENKTGQHSENSSIIK
                     TPKLEIRTQSGNQLNYVKSIRANDLSFGIGPAGTGKTFLAVALAVEALQDHFKRIILV
                     RPAVEAGESLGFLPGDLSQKVDPYLRPMYDALYEMLGFDQVHHLIERRAIELAPLAFM
                     RGRSLNESFVILDEAQNTTIEQMKMFLTRMGFGSRFVINGDITQIDLPVDKISGLKHV
                     LNVLSEIKSIGFTNFNYSDVVRHSLVQDIVEAYENHHE"
     gene            9390..9881
                     /gene="ybeY"
                     /locus_tag="QGH35_02660"
     CDS             9390..9881
                     /gene="ybeY"
                     /locus_tag="QGH35_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HJL81469.1"
                     /translation="MNQLDRQRVSVLIQNKTNHVLPLEANIIEECINLSVQASQEKLE
                     YPLAVNIRIVDHNEGLILNKKYRKRNQPTNVLTFEGNLDQERNLDIELPFLGDIVICL
                     PVVIQESRDQHKNLSAHFMHMLIHGFLHLLGFDHKKKDDETIMQSIENKVIEQSDIPI
                     WSK"
     gene            9888..10697
                     /locus_tag="QGH35_02665"
     CDS             9888..10697
                     /locus_tag="QGH35_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015436.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJL81470.1"
                     /translation="MNLWIKKLISLVKDKESNLESFFRMINSSQWNEFLGQDEIRMIQ
                     GVIEVSELQVRDIMIPRSNMVVLDIDLSIKDLLSQIARSGHSRFPVIGENKDEVVGIL
                     LAKDVLRFSTESDNNDFEITNFLRNPTFIPESKRLKTLLNEFRKSRNHMAIVVDEYGR
                     IAGLLTIEDVLEQIVGDIEDEYDAEDLPMIREKSAGTFEVDALIRIEDFNEYFETQYL
                     DEDFDTLGGMIMKNLGRLPHRGERISIEPMSFRIVKSDNRQVHLIEVRGIE"
     gene            10690..12180
                     /gene="lnt"
                     /locus_tag="QGH35_02670"
     CDS             10690..12180
                     /gene="lnt"
                     /locus_tag="QGH35_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HJL81471.1"
                     /translation="MNRHHVLSCIAGALFLLAFEPFDVPMLSIISIGYLFYLSINQPP
                     KIVAQCFFCFALVLFSSGLHWLYISIHTVSGAPIWLTVLLIFLLSSLMALYYYLTGLL
                     MSKIYQASQSKSLILLSIGPTLWVLMEWARGWLLSGFPWFSVGYSQINTFLSNWAPIG
                     GIYMVSWICAISSGLLVLIYLGAKQTSTKALFMLLVLLCTSWGVGVIQWTEPKSDKLN
                     VSLVQGAIEQEKKWLPSEFRNTLDLYKKTLQSEDQLDVVIWPEVAIPAISKNVKPYLN
                     EISTILEKNSIQLLLLGVLTSNEANGSVRNSMMAIGSSDVVYHKRHLVPFGEYFPVPN
                     FIRSWLKRMGLPNRDIESGASIQDMPQLNEVFIAPTICYEDIFGSEQLGYFPEANVLV
                     NITNNAWFGKSLAAEQHFQMSRMRAIETGRYLLRSTNTGVTAIVDPHGRIQKRSEPFT
                     FSILTGTFVPMSGVTPYTKYGDSLVLFLIILSLIFGYYWGIRRPKE"
     gene            12246..14705
                     /gene="leuS"
                     /locus_tag="QGH35_02675"
     CDS             12246..14705
                     /gene="leuS"
                     /locus_tag="QGH35_02675"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HJL81472.1"
                     /translation="MADKPYNPKEIESGVQAFWRENRVYEVSEDSNLDKFYCLCMFPY
                     PSGRLHMGHVRNYTIGDVISRYQRMLGKNVLQPMGWDAFGLPAEGAAIKNNMPPAQWT
                     RDNIEYMKHQLNLLGFGYDWTRELTTCDPNYYHWEQWMFSRLYEKGIAYKDNAIVNWD
                     PVENTVLANEQVIDGKGWRSGAKIERREMPQWFLKITHYCEELLSGLDNLDWPEPVKI
                     MQRNWIGRSEGVEIIFNIVDSEQTLTTYTTRPDTLCGATYMALAPEHPLVQSMSETNQ
                     DIKEFLEQTKLQAVSEEALEKMEKIGIPLGRNAINPINGNEIPIWAANFVLMSYGTGA
                     IMSVPAHDQRDYDFAKKYGLSIQPVIIPADPSIEHDFAKEAFQLPGQLINSEQYDGID
                     SIEAIAAITSELVKQKRGKKVVNYRLRDWLISRQRYWGAPIPMLSDENGKIFPECDQN
                     LPVVLPENVSFTGVQSPIKSMKEFTESVDPTSKKIHFRETDTFDTFMESSWYYARFCC
                     PDNDQKMLDERAKYWLPVDLYIGGIEHAILHLLYARFYHKLLRDEGLVEGDEPFKRLL
                     TQGMVLNGGVKMSKSMNNTVDPEEMINNYGADTVRFFMMFTAPPEQSLEWSDKAINGA
                     FRYLKKLWNIVHTRIDDLLDTEDIHQNDALNDTQKELRRRTHKTIAKVSDDIGRRYTF
                     NTAIAAIMELTNYVSSFKIETECDRKVIKEALESIVLLLSPIVPHICSYLWTELGHKN
                     AIIYENWPTFEKDLMIDEIHEIVIQINGKLKARINVMADIDEEALTKLALSEAKITQA
                     IGQKEIKKTIIVPGKLINIVI"
     gene            14708..15193
                     /gene="lptE"
                     /locus_tag="QGH35_02680"
     CDS             14708..15193
                     /gene="lptE"
                     /locus_tag="QGH35_02680"
                     /inference="COORDINATES: protein motif:HMM:NF016290.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HJL81473.1"
                     /translation="MLGKRLILILLSWSLFGCGFQLQDSSGLVAKLSTVYIDTSDPYT
                     IFYRSIKHHMAKNGIVVTNNKNGSDYVLSILADQSGQRVLSVSASNTPREYEVYYLVK
                     WSFSKDREVLIQPVTNKKIQDYGFDQTQLLGKSIESRVVKESLAKDIVQLIFYKIDQS
                     L"
     gene            15198..15782
                     /locus_tag="QGH35_02685"
     CDS             15198..15782
                     /locus_tag="QGH35_02685"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HJL81474.1"
                     /translation="MARLHFNYSTMNAGKSTALLQTNYGYLEKGMKTILYTSAKDTRY
                     GSSEIVSRIGIKSKSKTFKTEDNLFEQISIAHAKCAIDCVLIDEAQFLTKEQVAQLGR
                     VVDELDITVLAYGIRTDFQGELFEGSQYLLAWSDHLNEIRTVCHCGRKATMIVRVNEQ
                     GEIVREGEQIEIGGNERYLSLCRLHFDQGVTGKV"
BASE COUNT         5245 a         2531 c         3140 g         4879 t
ORIGIN      
        1 atcgtgacaa ttacagatag agcaggtaac gctttatctt gggcaacagc aggaggctca
       61 ggctttagag gctcaaggaa gagcactcca tttgctgctc aagttgcagc agaaaaggca
      121 gggaaagctg cacaggaatt tgggttaaaa aatcttgatg ttaaagtgaa aggtcctggc
      181 cctggaagag actcagcact gcgtgctttg aatgcaatag gttttagcat taacagcatt
      241 gaggatgtta cacctattcc tcacaatggg tgcagacctc caaaacaaag aagagtctaa
      301 ggaggattga taatggcaag atacttagga ccaaaatgta aactatctag aagagaggga
      361 actgatctct ttcttaaaag tgggataaaa ccaatagaaa ataaatgcaa gcttaatact
      421 gttcctggct caatggtagg atcaaggagg gacagattat ctgactatgg gcttcagtta
      481 agagaaaagc aaaaactgag aagaatgtac ggtgttttag agaaacagtt taaaaattat
      541 tataaaaaag cagcgaagat caaaggttca actggtgaaa atcttttaaa gttgttggag
      601 tgtcgtttag ataatatggt ctatagaatg ggatttgcaa taacgagagc tgaagcaaga
      661 caattggtga gccataaagc aattttggtc aataattcag tagtcaatat cccgtcatat
      721 caagtttcag aggatgatga aatatcaatt tctgagaagg cgaaagggca atttagaatc
      781 aagaatgcat tagatatttc aggacaattg ggtatttgtg attggttgtc agtggatgaa
      841 aagaaatttt ctgggacggt aaaatcaatc cctgacaggg aagatgtttt acctgatatt
      901 aatgagaatt tagttgttga attttactcc aaataaatag gagagttaca aatatggaag
      961 agtatggaga agtagaagta caagagatcg aaaaagaaga aatcgtttta gatttactca
     1021 acccaagagt gatcaaactc gagtcacagc atgaacttca gtctagggtt tcaatcgaac
     1081 cttttgaaag aggttttggg tatactttgg gtaatgcctt gagaagagta ttactatcca
     1141 gtttgcctgg ttatgcaatt acagaggtac aaatagatgg agtacttcat gaatatacaa
     1201 ccattgatgg agttcaagaa gatgttgtgg agattcttct gaatttaaaa aatgttgcaa
     1261 ttaatctaaa tgctcgcgac gaagccgaat ttgttatcag taaaaaaggt cctgctgtaa
     1321 taacagcagg tgatattcaa gttgatcatg atgccttagt tgttaatcct gaacatcaca
     1381 ttgctacgat tagttcaaaa aaagaaataa atatgcaatt gaaagttcaa aagggaaccg
     1441 gttatagacc agttactatc gatgcaaagc aagatgacga taatatagtg ggtcttttaa
     1501 agcttgatgc atcttttggt ccaatcaaga aggtaagcta cagggtagaa tcagcccgtg
     1561 tagaaaatag aacagattta gataaattga ttgttgactt agaaactgat ggaacaatag
     1621 aaccagagag tgcaataaaa tatgcaggag ctattcttca aaatcaactg agtatttttg
     1681 ttgacttgac tagggtacag gaacaacaaa aagcagaaga agaagtgctg atagatccta
     1741 tcatgttaag accagtcgat gaattagagc taacagttcg atctgcaaat tgtctcaaag
     1801 ctgaaaatgc aaactatatt ggagacctgg ttcaaaaaaa agaggtggag ttattaagaa
     1861 ctcctaattt aggtaaaaaa tccctgaatg aaattaagga agttttagcc agtcacggct
     1921 tgtcgttggg aatggagttg gaaatttggc ctcccaagga gttagtaaca gatgaagata
     1981 accaatagta atagaactat cgaattaaca aagatctaaa aaaaagatgc ggcataagaa
     2041 aactggaaga agattaggta gaaagagcga ccaccgtaaa gccatgtttc gcaacatggc
     2101 tgtttctctt attgtgcatg agtcaatatc aacaacattg cccaaagcta aagaattgag
     2161 aagaacaatt gaaccactaa ttacactagc aaaacaagat ggtgtttcta agagaagaag
     2221 agcttttgat aaactccgag ataaagcagc agtgggtaaa ctctttgatg aaataggtcc
     2281 aagattcaaa gaaagaacag gtggttattt gagaatactt aaaaaaggga tgagatcagg
     2341 tgactctgct caaatggctt tagttcaatt aactgattat gagattgagt tcagtgatag
     2401 tttgacagac gattagttat ctttcctact tgcaattgat ttttttctaa ccttcctatt
     2461 cttcttgttt tgtagagcaa caagaatata ttttatctaa tcacatagtt aactagttta
     2521 ttattctgtg tattattgta ttggagattc tcaacaataa agataataaa ataaaagcat
     2581 gatccaaaaa accatactga cgggaataac tacaacggga acaccacatt taggaaacta
     2641 tgctggcgtt attaggcagg ctattgatgc gagcttcaaa gatgagcatc gatcttttta
     2701 tttcttagca gattatcatg ccttgataaa aaattatgat ccaataaaaa ttaagcaatc
     2761 ttgtcttgaa gttgcagcta cttggctagc attaggattg gattttgata gaacattttt
     2821 ttatcgtcaa tctgatattc ctgaaattcc agagcttatg tggattttaa acacatttac
     2881 agctaaaggc ttgatgaaca gagcccatgc atataaatcg gcagtccaag ccaataaaga
     2941 atctgataca aaagatcctg ataaaggaat ttcagtggct ttatactcat atccagtatt
     3001 gatggctgct gatatcctaa tgtttaaatc aacgcatgtc ccagttggac aagatcaaaa
     3061 acaacaccta gaaatgacac gcgatatagc tcaacgattc aatcataatt atggagatat
     3121 atttgtaatt cctgaggtag tgattgaaag agatgcatct gttttaccag gattagatgg
     3181 tagaaaaatg agtaagagtt ataacaactc tataccactc tttctgccga gtggagaact
     3241 tagaaagtta attatgaaaa taaaaacaaa ttctttagaa cctggcgaac ctaaaacaac
     3301 aaaaaattgc ccattgtttg atatttttca aggcgtagcc acgcctgaag ccacatctaa
     3361 atttaaaaat aagctagaac aaggtttagc atggggtgag gcaaaagagg aattatttct
     3421 ttatatagag gatcttttct caggtcctag agaagaatat tttaagttaa tgaatgatcc
     3481 taaacatgta gataaactat taaagtctgg ggccgcaaag gctagagagt tatcacaacc
     3541 ttttctcaga gagattagag ctgcgatagg tatagattga ttagtttgac actcaaagaa
     3601 cctgtttaag gtactgacca gtataggaca gtttattttt tgcaacggtc tccggtgatc
     3661 cttctactat aatttcacca ccatgatctc caccttcagg gccaagatca attatccaat
     3721 cagcagtttt tataacatcc aagttatgtt caattaccac cacggtatta ccctcattgc
     3781 gtaattttat caatacctta agtagttgtt taatatcatg gaaatgaaga ccagttgtag
     3841 gttcatctag tatatataaa gtgccactat tacccctttt agaaagctct ttggagagct
     3901 tgattctttg tgcttctccc cctgaaaggg taacagcact ttgcccaagt tttatgtaac
     3961 caagacccac ttcatcaagt gttgccagtt tatttttaat tcttggaata ttggaaaaga
     4021 atctataagc atcactgaca ctcatttcaa gaacatcaaa aatattttta tttttgtaca
     4081 ttatttcaag cgtttctcta ttaaatcgtt tgcctttaca tgcatcacag gtcacgtaaa
     4141 tatctgctag aaaattcatt tcaactttaa tgacaccacc accctggcat gtttcgcacc
     4201 ttccaccttt aacattaaaa ctaaaacgtc ccggtttata tcctcttgat ctagattctt
     4261 gagtagagga gaacaattct ctgacgggtg taaataaacc agtataggtt gcaggattag
     4321 atcttggtgt tcttcctata gggccttggc ttatatctat cattttgtga atattaactg
     4381 gttcctcaat gaaagtttta gacaggtgat tgcagggttt cttttccaat gttgatgcta
     4441 ttaatggttg aagagtacca tttattaaag tagattttcc tgaaccagaa actcctgtaa
     4501 cacaggtgaa taaattcaat ggaatatcta catttagagc ttttaaatta ttcttctgag
     4561 cattttttat tttaataatt ttatttttgg caggagctaa tcttttggag ggcatcggaa
     4621 tagatttttc tttttttaaa tactggccag tcaatgattt tggttctttt agaagacact
     4681 ctaaattgcc ttgagccaca acctcaccac ctgcatttcc tgcaccagga cccaaatcaa
     4741 caatataatc acctgatctg attgctgctt catcatgttc aacaacaata acagtatttc
     4801 ctaaatctct aagttttgta agggtagcaa ttaatctttc attatctctt tgatggagac
     4861 caatagtagg ttcatcgagg atatacatta ctcctaccaa acccgatcca atttgacttg
     4921 ctagcctgat tctttgagat tccccacctg atagtgtctc agcactcctt tctagattaa
     4981 gataatttaa tccaacattt gataagaaca ataagcgctc ttgaatctcc ttgataatat
     5041 tggatgcaat ttcacctcga gttcctttaa tatttaattc ttgaaagaag gtcaaagctt
     5101 cgtcgatatt catttttgtt atatcggcta tggttttatt tttaataaaa acatgtctgg
     5161 ctgcttcatt taatcttgtt ccatgacaat ttaagcagct ttttacactc ataaattttg
     5221 ataaagcgtc tttgaccgct ttagattctg tttctttgta tcttcttatt aaattgggta
     5281 taacgccttc aaaaacatgc tttcttgata tttttctacc tcttcttcca ggataataga
     5341 actgaatttt ttcatctcca cttccatata aaagagtatt ttgaacggtt tcattatagg
     5401 attcatatgg tgtgtcaata ttgaaaccaa aatgttttga caagctttta atcaaataaa
     5461 aataataaat gtttcttcta tcccaacccc ttatagcacc ttctgaaata cttagtttag
     5521 gattctttat gacaagatgt ggactaaaaa attgttcaac tccaagacca tcacagcttt
     5581 gacaagctcc tgcagggtta ttaaatgaga atgacctagg ttctagttct tgaatggaat
     5641 agccacagac gggacaagca aatcgatcag aaaataacaa cattggatga tctttctctt
     5701 ggttaacaat cttggcaacg ccatctgcca tttccagagc tgtttcaaaa gattcagtta
     5761 atcgaagagc aatatcaggt ttaactgtaa atctatcaac aacaacttca attgtatttt
     5821 tcttcttcga atcaatagtt ggtaaatcat cgatatcgta tattttttgg ttaattctga
     5881 cccttatgaa acccttagct tttattgttt tgattaattc aacgtgttct ccctttctgt
     5941 cattaacgat gggtgataat agaactaatt ttgatccttc ttttatttgt aatacgtgat
     6001 caaccatctc actgatattt tgtatgccta actcgatacc gtgttcggga cactttggaa
     6061 tgccggttct cgcataaagt aagcgaagat aatcatatat ctcagtgacc gtaccaactg
     6121 ttgatcttgg attgtgagaa gtcgtctttt gttctataga aattgcaggt gaaagtcctt
     6181 cgatcaaatc aacatctggt ttttccatca ttgataggaa ttgtcttgca tatgctgaca
     6241 aagattcaac ataacgtctt tgaccttcag cgtaaattgt atcaaatgcc aatgaagact
     6301 taccagaacc agaaaggccc gtcaggataa taattttgtt tctaggcaac tctaggtcta
     6361 aattttttaa attatgtgtt tttgcccctt tgattctaat atgttgcatc tttacactta
     6421 taataatttg taccaaacat tcaatgataa atcttttcaa gctgaagagc caaacctaac
     6481 ttaaggtttg agagacaata gatacattgt gttcaggata gttatttttt tatcgatctt
     6541 aagcacaatc ttttagcttt ttgtttacaa taaccattgg aataatgaat ctttagatga
     6601 ggtaaggaaa taatatggct agaggaatta ataaggttat tgttttaggt aatttgggtg
     6661 ctgatcctga cgcccgttta atgcctaatg gaaatgcagt tacaaatatt agtgttgcta
     6721 ccagcgaatc atggattgat aaagaatctg gagatcgcca agagagaacc gaatggcaca
     6781 gggttgtttt ttttggacga ctggcagaaa tcgccagcga atatctaaaa aaaggctctc
     6841 aagtttatat agagggcaaa ctcagaacta gaaaatggga agataaagag ggaaatgaac
     6901 gttggacaac tgaaataata gccaatgaaa tgcagatgct tggtgaacgc atgacttcct
     6961 ctgccagtac tgataatttt gagacaccta aacaaagtgc caataatgct tcatcagatg
     7021 attttgatga cgatattcct ttttaatact accagtaatt cagcttatta aatgtgtctg
     7081 gaaagatatt tataaaatct tttggctgtc agatgaatga atacgatgcc agtaagatgg
     7141 ccgatatcat gtgtcatgat caagatatga aactcaccga tgatccatca aaggcagatc
     7201 tattattgtt gaatacatgt tcagtaaggg agaaaccaca agaaaaagtg ttctcacaac
     7261 ttggtagatg gcgtgcgtac aaaaaaaata atcccaatat tataatcggt gtgggtggat
     7321 gtgttgcaac tcaagagggt gaatcaatta ttaaaagagc accctatgtt gatatggtct
     7381 tcggaccaca aacactgcac cgcctaccaa aaatgtacga tgaagtaatt gcatcgagac
     7441 attcagttgt tgatattagt tttccaaaat tagagaaatt taattttttt ccaaaaacaa
     7501 atcaaatcaa accttcagct tttgtttcga ttatggaagg atgtagtaaa ttatgctcct
     7561 tttgtgttgt tccttatacg agaggtttgg aagtgtgtcg gtttccagag caggtgatgg
     7621 aagagatcca aggtttagct tctgaaggag tcagagaagt gaatcttttg ggtcaaaatg
     7681 tgaatgccta taggacaaaa gatatacaag gcaataaaat aaaattatct aatctgattg
     7741 agcaagtaag tacaataata gggattgatc gtatccgttt taccacatct catccgatac
     7801 aatttagcga agatttgatt caatcatttg ctaatccaaa agtggctaat tttcttcatc
     7861 ttccagtaca aagtggatcc aataaaatat tacaattaat gcaaaggaag catagcgtcg
     7921 aagcttatat tgataaaatt gaacgactaa gagatcttag acctgatatt agtgtttcct
     7981 cagattttat tgttggattt ccaggtgaga ccgaagacga ttttgagcaa actctaacat
     8041 tgattgatac tataggtttt gatcaatcat tcagttttat ttttagtcca agacctaata
     8101 catctgctgc aagcatggaa gatacaattg gccatgatat aaaacttgcc agactccaaa
     8161 cattacaagc caaaataact gaacatgcaa tggcaatcag tagaaaaatg attggagcta
     8221 aagagagagt cctagtagaa aagcaatcta agaaaatcaa aacgcaaatg actggtagaa
     8281 cagaaaataa tcgctgggtt aactttgacg gtcatcaaga tttaattgga cagtttgttg
     8341 atctcatcat aactgaggca ctacctaatt ctcttagagg tagattaact taataataat
     8401 tttatatagg attttttgac ttgaaccata aagaatttga aatagattca acagataata
     8461 ataatcttgt aaatttgcta ggtgtgtttg ataaaaactt acgagtgatc gaagattttt
     8521 tgtccataga aatcaagaat aggggcaata ttttcttgtt gtcaggtcaa agagaagatt
     8581 taataaaagg tgaggaaatc attaggcatt tatataaagc tgcttctaat gaaaatctat
     8641 cagagaggaa aatacattta ttgttgagaa agtatatggg agagaataaa actggccaac
     8701 atagtgagaa tagctcaatt attaaaacac ctaagctgga aatcagaacc caaagtggta
     8761 atcaactaaa ctacgttaag tctattagag ctaatgattt gtcttttgga ataggaccgg
     8821 ctggaactgg taaaactttt cttgctgttg ctttagcagt tgaggcactg caagaccatt
     8881 ttaaaaggat aattcttgtt agacctgctg ttgaagcagg tgaaagcctt ggttttctac
     8941 caggtgattt gtctcaaaag gtggatcctt atcttagacc aatgtatgac gccttatatg
     9001 aaatgctagg ttttgatcaa gtgcatcatt tgatcgaaag aagagcaatt gaattggctc
     9061 cactagcttt tatgcggggt agatcgttga atgagagttt tgtaatttta gatgaagcac
     9121 aaaatactac gattgaacaa atgaaaatgt ttctaacccg tatgggcttt gggtctagat
     9181 ttgtaattaa tggtgatatc acccaaattg atctgccagt agataaaatc tccggactga
     9241 agcatgtatt aaatgttttg agtgaaatca agagtatcgg ttttaccaat tttaattatt
     9301 ctgatgtagt gagacacagt cttgttcaag atattgtgga agcttacgag aatcatcatg
     9361 aataggctct cttataggtt taattttcta tgaatcaact tgatcgtcaa cgagtaagtg
     9421 tactgatcca aaataaaacc aaccatgtcc ttcctttgga agctaatatt atagaggaat
     9481 gtattaactt gagtgttcaa gcttcacaag aaaaactaga atatccattg gctgtcaata
     9541 ttaggattgt tgatcacaac gaaggattga tattgaataa gaagtataga aaaaggaatc
     9601 aacctacaaa tgttttgaca tttgaaggta atttggatca agagagaaat ttagatattg
     9661 aactcccttt tctaggtgat attgttattt gcttgcctgt ggtgattcaa gaatctagag
     9721 accaacataa gaatcttagt gcacacttta tgcatatgct gatacatgga tttttacact
     9781 tgttgggttt tgatcataaa aaaaaggacg atgaaacaat tatgcagagt attgaaaata
     9841 aagttataga acaatcagat atcccgattt ggagtaaata atctcgaatg aatttatgga
     9901 ttaaaaaatt gatttctcta gttaaagaca aggaaagtaa tcttgaatca ttttttagga
     9961 tgattaattc cagccaatgg aatgagttct tagggcaaga tgaaattaga atgattcaag
    10021 gtgttattga agtttctgaa cttcaagtga gagacataat gattccccgt tccaatatgg
    10081 tcgttttgga tattgattta tctatcaaag atcttttgag ccaaatagca cgatcaggtc
    10141 attctaggtt tccagtcatt ggtgaaaata aagatgaagt tgttgggata cttctagcaa
    10201 aagatgtatt gagattttca acggaaagtg acaacaatga ttttgagata actaattttc
    10261 ttaggaaccc aacttttatt ccagaaagca aaagattgaa aaccttactc aatgagttta
    10321 gaaagagtag aaatcatatg gctattgtgg tggatgaata tggaagaata gcaggactat
    10381 tgactattga agatgttcta gagcaaattg taggagatat tgaagacgaa tacgatgctg
    10441 aagatctgcc gatgatacga gagaaaagcg ctggtacatt tgaggttgat gccttgattc
    10501 gaattgaaga ttttaacgaa tattttgaaa cccagtattt ggacgaagac tttgataccc
    10561 tcggaggaat gattatgaaa aatctaggaa gacttcctca cagaggtgaa cgtatatcaa
    10621 ttgagccaat gagctttaga atagttaaat cagataacag gcaagtgcat ctgatagaag
    10681 tgagaggcat tgaatagaca tcacgtattg tcctgtatag caggagcatt atttttgttg
    10741 gcttttgaac cttttgatgt gcctatgcta tcaattattt ctattggcta tttattttat
    10801 ttgtcaataa atcaacctcc aaaaattgta gctcaatgtt ttttttgttt tgctttagtg
    10861 ttattttcat cgggactcca ttggctatac ataagcattc atactgttag tggcgcacct
    10921 atttggttga cagtattgtt aatttttttg ctgtcgtcat tgatggctct gtattattac
    10981 cttacaggtt tattgatgag taagatttat caggcaagcc aatccaaatc actaatctta
    11041 cttagcatag ggcctacgct gtgggtttta atggaatggg ctaggggttg gttgttatca
    11101 ggatttcctt ggttcagtgt gggttatagt cagatcaata cttttctttc taattgggca
    11161 cctataggtg gcatttatat ggtgagttgg atctgtgcaa tatcttctgg cctgttagtt
    11221 ctcatttatt taggcgccaa gcaaaccagc actaaggctc tgtttatgtt gctggttctt
    11281 ctatgcactt catggggagt gggagtaatt caatggaccg aaccaaagag tgataagctc
    11341 aatgttagtt tagttcaagg agcaattgag caagagaaaa agtggctgcc cagtgaattt
    11401 agaaatacac tagatttata caaaaaaact cttcaatctg aggatcagtt agatgtggta
    11461 atctggcctg aggttgcaat tccagctatt tcaaaaaatg ttaaacccta ccttaatgaa
    11521 ataagcacaa tcttagaaaa gaatagtatt cagctattgt tattaggcgt tcttacctct
    11581 aacgaggcaa acggcagtgt tagaaattca atgatggcaa ttggctcaag cgatgtggtt
    11641 tatcataaaa ggcaccttgt gccttttgga gagtattttc cggttccaaa ttttattaga
    11701 tcttggctca aaagaatggg tcttcccaac agagatatag aatcaggcgc aagcatacag
    11761 gatatgcccc aattaaatga ggtctttatt gctccgacga tttgttatga ggatattttt
    11821 ggttcagagc aattaggtta tttccctgaa gctaatgttt tggtaaatat tactaataat
    11881 gcttggtttg gaaaatccct tgcagcagag caacattttc aaatgtcaag gatgagagcc
    11941 atagaaacgg gtcgttatct tctacgatct accaatacag gtgtcacagc gattgttgat
    12001 cctcatgggc gtattcaaaa acgctctgaa ccttttacat tttcaatcct aacgggaaca
    12061 tttgtgccta tgagcggtgt aactccttac acgaagtatg gagactcatt agtattattt
    12121 cttatcatat tgagtttaat tttcggctat tattggggta taagaagacc caaagagtaa
    12181 tacagtttcc actaattact atcccgatgt atgcttgata actgtttttt taaatgaaat
    12241 taaacatggc agacaaacca tacaatccaa aagaaataga atccggagtt caagctttct
    12301 ggagagaaaa tcgtgtttac gaagttagtg aagactctaa tttagataaa ttctattgtt
    12361 tatgtatgtt tccttatccg agcggaagac tgcatatggg tcatgtacgg aattacacca
    12421 taggtgatgt tatctcaaga tatcagagaa tgttggggaa gaatgtattg caaccaatgg
    12481 gttgggatgc ttttgggttg cccgcagaag gagctgcaat taagaataat atgcccccag
    12541 ctcaatggac cagagacaat attgaataca tgaaacacca attaaacctg cttggatttg
    12601 gttacgattg gactagagaa ctaaccactt gcgatccaaa ttattatcat tgggaacaat
    12661 ggatgttctc ccgtttatat gaaaaaggta ttgcctataa agataatgcc atagtcaact
    12721 gggatcctgt tgagaatacg gtattggcaa atgaacaagt gatcgatggc aaaggatgga
    12781 gatcgggtgc aaagattgaa cgacgagaaa tgcctcaatg gtttttaaaa attacccatt
    12841 actgtgaaga attgctttca ggtttagaca atcttgattg gccagaaccc gttaaaatta
    12901 tgcaaaggaa ctggataggt agatccgaag gggttgaaat tattttcaac attgttgatt
    12961 cagaacaaac attaacaacc tacactacga gacctgatac cttatgcggt gcaacttata
    13021 tggctttggc acccgagcat cctcttgtgc agtcgatgtc agagactaat caggatataa
    13081 aagaattttt agagcaaaca aaactccagg ccgtttctga ggaggctctg gagaaaatgg
    13141 aaaaaatagg gattcctttg ggacgaaatg caatcaatcc aatcaatggt aatgaaatcc
    13201 caatatgggc ggctaacttt gtcttaatga gttatggaac tggagcaatt atgtctgttc
    13261 ctgcgcacga tcaaagagat tatgattttg ccaaaaaata tggcttatct atccaacctg
    13321 taatcattcc agcagatccg agtattgagc atgattttgc aaaagaagct tttcagttac
    13381 ctggtcagtt aatcaattcc gaacagtatg atggaataga ctccatagaa gccatagcag
    13441 caatcacttc cgagttagtg aaacaaaaga gagggaaaaa agtggttaat taccgcttaa
    13501 gggactggtt gatttcaagg caaagatatt ggggagcacc catcccgatg ctatcagatg
    13561 aaaacgggaa aattttccct gaatgtgatc agaatttacc agttgtactt cctgaaaatg
    13621 tttcgtttac aggtgttcag tcgccaatta aatcaatgaa agaattcaca gagtcggttg
    13681 atcccacatc aaaaaaaatc catttcagag agactgatac gtttgatact tttatggaat
    13741 cttcttggta ttatgcacga ttttgctgtc cagataatga tcagaaaatg ttagatgaaa
    13801 gagcgaaata ttggttaccc gttgacttat atataggcgg tattgagcat gctattcttc
    13861 atctgcttta tgctcggttt taccataagc ttttaagaga tgaaggtttg gttgaaggag
    13921 acgagccttt taaacgttta ttgactcaag gcatggtact gaatggcggt gtcaagatgt
    13981 cgaaatccat gaataacact gttgatccag aagagatgat taataattat ggtgccgata
    14041 ctgtcagatt ttttatgatg tttaccgcac ctcctgagca gtcattagaa tggtcagaca
    14101 aggcgatcaa tggagctttt cgttatttga aaaaattatg gaatattgtt cataccagaa
    14161 tagacgatct tttagataca gaagacattc atcaaaatga tgctttaaat gacacacaaa
    14221 aagaacttag aaggcgcact cacaaaacta tagccaaggt gagtgatgat ataggtagaa
    14281 ggtatacttt taatactgca atagccgcaa ttatggaatt aactaattac gtatcgagct
    14341 ttaaaataga aacagaatgc gatcgaaagg tcatcaagga agctttagaa tcgattgtat
    14401 tattactctc acccattgtg ccccacattt gcagttattt atggactgag cttggtcata
    14461 agaatgccat catttatgaa aattggccta catttgaaaa agatctaatg attgatgaaa
    14521 tacacgaaat tgtcattcaa attaatggca aattgaaagc acgcatcaat gttatggcag
    14581 acattgatga agaggcgctt acaaagttag cgcttagtga ggcaaagata acccaggcta
    14641 taggacaaaa agaaataaag aaaactataa tcgtgccagg gaagctaatt aatattgtta
    14701 tttgatcatg ctaggtaaac gactcatact cattttgtta tcttggtcgc tttttggctg
    14761 tggcttccag ttacaggata gcagcggttt agtagcaaaa ttaagcaccg tatatataga
    14821 tacttccgat ccatatacta ttttttatcg atcgattaaa catcatatgg caaagaatgg
    14881 tatcgttgtg acaaataata aaaacggttc agattatgta ttatcgattc tagcggatca
    14941 atctggacaa agagttttat ctgtatcagc aagcaacaca ccgagagagt acgaagttta
    15001 ttatttggtt aaatggtctt tcagtaaaga tagagaagtt cttatacagc ctgtgactaa
    15061 caaaaaaata caagattatg gctttgatca gacacaatta ttgggaaaat caattgaaag
    15121 tcgtgttgtg aaagagtctt tggccaaaga tattgttcaa ttgattttct ataaaataga
    15181 tcaatctttg taaaatcatg gctcggctac attttaatta ctcaaccatg aatgcaggaa
    15241 agtcgactgc actgttgcaa actaattatg gctatcttga aaaggggatg aaaacgattc
    15301 tgtacacctc cgctaaagac actcgatatg gcagttcaga aattgtttca agaataggaa
    15361 taaaaagtaa atcaaagaca ttcaagacag aagataattt atttgagcaa atatcaatag
    15421 cacatgcaaa gtgtgcaata gattgtgttt taattgatga agctcagttt ttaacaaaag
    15481 aacaggtggc ccaattgggt cgtgtcgttg atgagctgga tattaccgtt cttgcttacg
    15541 gcataagaac agacttccaa ggtgaattat ttgaaggtag tcaatatttg ttggcttggt
    15601 ctgatcatct caatgaaatt agaaccgttt gtcactgtgg acgtaaggca accatgatag
    15661 ttcgtgtgaa tgagcaggga gagattgttc gagaaggaga acaaatagag ataggaggca
    15721 atgagcgata tttatctttg tgtcgactcc attttgacca aggagttacg ggtaaagtct
    15781 aagagattgc tccga
//
LOCUS       DASCTH010000127         1785 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000174707, whole genome shotgun sequence.
ACCESSION   DASCTH010000127 DASCTH010000000
VERSION     DASCTH010000127.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000174707"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1785)
                     /locus_tag="QGH35_02690"
     CDS             complement(<1..>1785)
                     /locus_tag="QGH35_02690"
                     /inference="COORDINATES: protein motif:HMM:NF017591.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HJL81475.1"
                     /translation="NDHIGVVETSNHQAFLVVNHETIDYDNFETYLETFPMVMGYSLP
                     DEVFDSIEGDFIWNFPEMSETNPAKAMIKTIAMEASDDMGISVISVERKSNGDWVRTY
                     SDQDRRISITQALKDPAKLSKSSGPARAIFIKENKIGFDDGLGDKCVGTYWNCSGTTT
                     PWGTVISAEERLDSHVYEPVKADGSSFPPTTIPFHPKTANGLGSIFELAANKYGWAVE
                     VDPANKSDFGTKHTMLGRYRHEAFAINCKENKPLAIFSGCDRKGGHIYKFISTDDVVD
                     CKSKSNSKLLEKGVLHVAKFAADGTGYWIALTPDTDIDPILPSGVIGNTVSLPNPDRV
                     EGGVKKYQKDEDVRADYQTLGSKLGDLYQGNDKTELQGAILIDAHYAANAVGATGCPR
                     PEDCEFDEKKGAVYYALTAITDGSSDSPTKEIFARDDYKEKEANLADSQKDHYRPGMI
                     IKIIDDENGDPESLTFQWTTLLMGGEPSDDKAGWVSPDNLEIDGKGNLWMVTDISTET
                     LNVSVVNRAEVSRNAMRGIHGNNSAWFIPTSGEFVGQSLPFAMGPTESELCGLKFSAD
                     QKTLFLTPQHPGLLNGMRKNMAYEEREF"
BASE COUNT          484 a          433 c          315 g          553 t
ORIGIN      
        1 taaattctct ttcctcataa gccatatttt ttctcatccc atttaataat ccagggtgtt
       61 gtggggtgag aaaaagggtt ttttgatctg cactaaattt taatccacat aattctgatt
      121 ccgtaggtcc catagcgaaa ggtaaagatt gacccacaaa ctcaccagaa gttgggatga
      181 accaagcact gttattacca tgaatccctc gcattgcatt tcgtgatacc tcagctctat
      241 taaccaccga tacattcaga gtctctgtag aaatatcggt caccatccat agattgcctt
      301 tcccatcgat ctcaagatta tctggactaa cccaacccgc cttgtcatcg gatggctcac
      361 cccccatcaa taatgttgtc cattgaaaag tcaaagattc gggatcacca ttctcatcat
      421 caataatttt tataatcatt ccaggtcgat agtgatcctt ttgactatct gccaaattgg
      481 cttccttttc tttataatcg tctcgggcaa aaatctcttt agtggggctg tctgaggatc
      541 catcggttat tgcagtaagc gcataataga cggcaccttt tttctcatca aactcacaat
      601 cttctggtct gggacaccct gttgcaccaa ccgcattagc agcatagtgt gcatcgatta
      661 atattgcgcc ctgcagctca gttttgtcgt taccctggta caaatcacct aatttagaac
      721 ccagagtttg ataatcagct ctgacatctt catctttttg atatttcttc actccacctt
      781 ctacccggtc tggatttgga agagaaactg tattaccaat cacaccactg ggtagtattg
      841 gatcaatatc agtatctggt gtcagcgcga tccagtatcc tgttccatca gctgcaaact
      901 tggctacatg caatactcct ttttccagta actttgaatt acttttagac ttgcaatcga
      961 caacatcgtc tgtgctgatg aatttataaa tgtgaccccc tttgcgatca cagcctgaga
     1021 atatagccaa tggtttgttc tccttacaat tgattgcgaa agcttcgtga cgataacgtc
     1081 ccaacatggt gtgcttggta ccaaaatcac ttttgttagc tggatccacc tcaacagccc
     1141 aaccatactt attggctgcc aattcaaaaa tacttcccaa accattggcc gtttttggat
     1201 gaaagggaat ggtcgttggt ggaaatgaac taccatcagc tttaaccggt tcataaacat
     1261 gactgtccaa tcgttcctct gcgctgatca ccgtccccca aggagtggtt gtaccagaac
     1321 agttccaata ggttccaacg catttatcac ccaaaccatc atcaaaacca attttatttt
     1381 ctttaatgaa aatagcccta gcaggaccac tagatttgga taatttggca ggatctttaa
     1441 gtgcctgggt gattgaaatt cttctatctt gatccgaata agttctaacc caatcaccat
     1501 tgctctttct ttcaacagaa ataaccgaga ttcccatatc gtctgacgct tccatggcaa
     1561 tggttttgat cattgcctta gcaggatttg tttcactcat ctcgggaaag ttccatatga
     1621 agtctccctc gattgaatca aacacttcat ccggtaagct atagcccatc accattggga
     1681 aagtctccaa ataggtctca aaattgtcgt aatcaatggt ctcatggttg acgactaaga
     1741 acgcttgatg attgcttgtc tcaacgactc cgatgtggtc attat
//
LOCUS       DASCTH010000128         2181 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000176757, whole genome shotgun sequence.
ACCESSION   DASCTH010000128 DASCTH010000000
VERSION     DASCTH010000128.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000176757"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="QGH35_02695"
     CDS             <1..192
                     /locus_tag="QGH35_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81476.1"
                     /translation="NYIGDFSFQKIERRSEKRPSFAKPFCNNLARSSYMNNMGMAGSF
                     WGPTSMSVLRLQCLCRQCS"
     gene            complement(243..641)
                     /locus_tag="QGH35_02700"
     CDS             complement(243..641)
                     /locus_tag="QGH35_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81477.1"
                     /translation="MKNLLLGIIAILFSFNGWAEQSNPCHLYYPNIPGELVLENDKLV
                     VQRFIIQPGQWEGIHSHPGNQVYIHIKGGEWSGRIGGKSEYSGEIDEDGSVGWMDAIP
                     LSAGHDSGNTGDEPIDIIYVTLKTDCMDGN"
     gene            complement(700..790)
                     /locus_tag="QGH35_02705"
     tRNA            complement(700..790)
                     /locus_tag="QGH35_02705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(754..756),aa:Ser,seq:gga)
     gene            complement(859..1803)
                     /locus_tag="QGH35_02710"
     CDS             complement(859..1803)
                     /locus_tag="QGH35_02710"
                     /inference="COORDINATES: protein motif:HMM:NF014715.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HJL81478.1"
                     /translation="MQPGGNNLLVIDLGGTNIRAAFGSKDGKGLEHIQKIKLDRIEHF
                     YEILDQLISTRKTGVDHVVISVAGPKNGNYITMTNRDWKISAEELKAKFNLNSCDLLN
                     DWEAIAYSLPCLQDQDLNSIKVGEYVAINDGPKIIIGPGTGLGAAMYSKINDYEYVNA
                     TEIGNTQTSVSHYLNVFGIKQTDDFTILEDVLSGPGLTKLYNILHDESITAEEILRRL
                     NEVNDSRSIELINNYIKCFAKLSSEMALTYNCVGGIFLAGSLMRGIEDYFDTQVFTEN
                     FIGERKSIHQEFLRKIPVFLVKKEFTPLYGSQNYFLNH"
     gene            1982..>2181
                     /locus_tag="QGH35_02715"
     CDS             1982..>2181
                     /locus_tag="QGH35_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HJL81479.1"
                     /translation="MIELYYAPTPNGWKITIMLEECSLPYKIIPVNLREGDQFKPEFL
                     AISPNNRMPAIVDHNHGQKGDS"
BASE COUNT          677 a          438 c          371 g          695 t
ORIGIN      
        1 aactacattg gtgattttag ctttcagaaa atcgaaaggc gttccgaaaa acgcccttct
       61 tttgctaaac ccttttgtaa taatttagcg aggtcatcat acatgaataa catgggaatg
      121 gcaggttctt tctggggacc aacgagtatg agtgttttgc gattgcaatg tctttgcaga
      181 cagtgcagtt aaaaaagctt tgtcctatcc taaatactga acctgaactg gttctggttt
      241 agctaattgc catccatgca atctgttttc agtgttacat aaattatatc aataggctca
      301 tctccagtat tgccagaatc gtgtccagca ctgagtggga ttgcatccat ccagcctaca
      361 gacccatcct catcaatctc tcctgaatac tcagattttc caccgattcg gccagaccac
      421 tccccaccct tgatgtgtat gtaaacttga ttaccaggat gactgtgaat gccttcccac
      481 tgtccaggtt ggattatgaa cctctgaaca acgagtttgt cattttccag cactagttca
      541 ccaggaatat tgggataata aagatgacag ggatttgact gttcagccca accattaaag
      601 ctgaagagta tggcaataat gccaagtagt aggtttttca tttgtttctc ctggttaatt
      661 tctgtgagtc aatggtagta gtggtttcag agcttttaat ggcggagaga gagggattcg
      721 aaccctcgaa gggctattaa cccttacacg ctttccaagc gtgcgcaatc gaccactctg
      781 ccatctctcc tttcttaatg ctgattaagt ttcaaaccag cagtatttcc agaacatata
      841 gcaagtaagt ctatattgtc aatggtttaa aaaataattc tgactaccat aaagtggtgt
      901 gaattctttt tttactaaaa agacagggat ttttcgtaag aattcttgat gaattgattt
      961 tctttcaccg ataaagttct cagtaaatac ctgagtatca aaatagtctt caatacctcg
     1021 catcaaagaa ccagctaaaa atataccacc aacacaatta taggttagtg ccatctccga
     1081 gctaagcttg gcaaaacact taatataatt attaatcagt tcaatagatc gagaatcatt
     1141 aacttcgttt aagcgcctca gtatttcttc agcagtgata ctttcatcat gaagaatatt
     1201 gtataacttg gttagacctg gacctgaaag cacatcttct agaatggtaa aatcatccgt
     1261 ttgttttatg ccaaatacat ttagataatg actcactgaa gtttgggtgt taccaatttc
     1321 cgtggcgttc acatattcat agtcattaat tttagaatac atagccgcac ccaaccctgt
     1381 acctggacca ataataattt taggtccatc gtttatagct acatactctc cgactttgat
     1441 agagttaaga tcttgatctt gaagacacgg cagtgaataa gcaatggcct cccaatcatt
     1501 tagtaaatca caactgttta agttaaattt agcttttagc tcttctgctg atattttcca
     1561 gtcacggttg gtcatagtaa tataattacc attttttggc ccagcaactg aaatgactac
     1621 atgatcaaca ccggtttttc ttgttgagat caactgatct aaaatttcat agaaatgctc
     1681 tatacgatcc agtttaattt tttggatatg ttctaaacct ttaccatctt ttgagccaaa
     1741 cgcagctcgg atgttagttc ctccaagatc aattactagg agattattac caccaggttg
     1801 cataaaatac tactaaaatc atgacgacta taactgaaac aatattaaag aatgatgaag
     1861 ttctaaaaga tacttcatct aaaaagactt ttcaaatcta tcgagattca ccaattgttg
     1921 actgacatga aaatgtaatt tcttctttat tttaatgcaa attctaatag gataatcaat
     1981 tatgattgaa ctttattatg cccctactcc caatggttgg aaaattacca tcatgttaga
     2041 agaatgttca cttccctata aaattattcc agtcaattta agagaaggtg atcaattcaa
     2101 gccagagttc ctagctataa gtcccaacaa tcgaatgcca gcgatcgtag atcataatca
     2161 tggtcaaaaa ggtgactcta t
//
LOCUS       DASCTH010000129         3845 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000177578, whole genome shotgun sequence.
ACCESSION   DASCTH010000129 DASCTH010000000
VERSION     DASCTH010000129.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000177578"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="QGH35_02720"
     CDS             <1..1002
                     /locus_tag="QGH35_02720"
                     /inference="COORDINATES: protein motif:HMM:NF020129.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HJL81480.1"
                     /translation="QMGTSGEPTQGSGAAAILIESNPKIAEVHTNISGSASNYRLIDF
                     RKPIHHRAKNSHKHSDLNLELPIYNGKYSATCYIDETINALADMCQKRNLNTAQHLRQ
                     IPALFMHRPFHRMPINAFTIIYLYALSIGNSDDLDELSGYANIADVNHHDLFQELQQR
                     PNISEYPLIGKINYEPLPLTQIVAKTCQKEKNFQDLILHKLDFGSELTKEMGNIYSGS
                     VFGWLAAGLEDAIANNIDWTHKEALLFGYGSGDAAEVIPISFVDDWQTAASKVKFSQS
                     FGDCYNLSYSQYTDLRSDKKLNGIDHKLNKQFVVKKIGTEERDDFQDAGIEYYEYLN"
     gene            1120..3219
                     /locus_tag="QGH35_02725"
     CDS             1120..3219
                     /locus_tag="QGH35_02725"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008304489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HJL81481.1"
                     /translation="MKNCTTLLFVTAFILTSCQADYNKPVEIAAVDAERIINADREPQ
                     NWLAHGRTYDEQRFSPLTTINADSISKLGLAWYLDLGTNRGLEATPIVVDGVIYVSGA
                     WNIIYAIDARSGKLLWQHDPETSRAWVAEYAVHDAVSRGIAVWDGKVIAATLDGRLLA
                     LDATNGELLWSVLTIDRSKPYTITGAPRIVKDRVIIGNSGADYGVRGYLSAYDVSNGE
                     LDWRFYTVPGDPLKPQESAVLERVVKTWTGDEWYKTGGGGTVWNSMAFDPEMNLLYFG
                     VGNGTPWSRELRSPGGGDNLFLSSIVAVNPDNGEYVWHYQTTPGESWNFSAVESIILT
                     ELEIEGKLRKVLIQAPKNGFFYVLDRATGELLSAQQYIEQNWASHVDMATGRPVEYPF
                     ARYKNQGRLIVPGPSGGHSWYPMSYSPHTKLAYIPALSVPGYYNHQEAYAHQPGFQNK
                     GIEEGVSDFRALNPQGERQKIRFGMRLLAWDPQTQTERWRVEHTDLPGGTLAMTGNLV
                     FQGLGSGEFAAYAADTGTKLWSFEAGTTIMAGPVTYAVDGEQYVAVLAGRGGGAGLVG
                     GPTGVHWNGLVNENRLLAFKLGGLTKIPSARKLVRHVSIPDELRSTTEDKALIGRGQK
                     IYEQYCYMCHGLGVFSGGVIPDLRYANSQTFANWEAIVIGGSRSAQGMRSFEGALSAE
                     DALAIRAYVLRQAVKLQ"
     gene            3254..>3845
                     /locus_tag="QGH35_02730"
     CDS             3254..>3845
                     /locus_tag="QGH35_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018050757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81482.1"
                     /translation="MKKSISIIQEMNQFQKLMQEWRRDIHQHPELAFNENRTAELVAK
                     TLESFGVEVFTGIAKTGVVGKLSSGNSDNAIGLRADMDALPMDEDNTFDYRSQNDGVF
                     HGCGHDGHTAMLLGAAKYLSSTKEFDGTIYFIFQPAEEAAGGAPEMIKDGLFENYKMS
                     SVYGMHNMPGMPVGMFGIRSGHMFAAFAPFDIEIRGI"
BASE COUNT         1121 a          762 c          926 g         1036 t
ORIGIN      
        1 caaatgggta cgagtggtga gccaactcaa ggttcaggtg cagcagctat cttgattgag
       61 tctaatccta aaattgctga agtacatact aatatatctg ggagtgcttc taattaccgt
      121 ctcattgatt tcaggaaacc catccatcac cgggcaaaaa actcacacaa gcattcagat
      181 ctcaatctgg agctaccaat atataatggt aaatattcag caacttgtta cattgatgaa
      241 accattaatg cattggctga tatgtgtcaa aagaggaatc ttaatacagc acagcatctg
      301 agacaaatac cggctttatt tatgcacagg ccttttcata gaatgcctat caacgctttc
      361 acaataattt atttgtacgc actatctata ggcaatagcg atgatttaga tgaattatca
      421 gggtatgcaa acattgcaga cgttaatcac catgaccttt tccaagagct gcaacaaagg
      481 cccaacatta gtgaataccc cttgattgga aaaattaatt atgaaccatt acctttgaca
      541 caaatagttg caaaaacctg ccagaaagag aaaaacttcc aggatctaat tttacacaaa
      601 ttagactttg gttcagagct caccaaagaa atgggcaata tttattcagg ttctgttttt
      661 ggctggctgg cagctgggtt agaagatgca attgctaata atatcgactg gacacataaa
      721 gaagctcttt tgtttggtta tggaagtggt gatgctgctg aagtcatacc catttctttt
      781 gttgatgact ggcaaacagc agcatcgaag gtgaaatttt ctcaatcttt tggtgattgc
      841 tataatcttt catatagtca atatacagat ttaagatctg acaaaaaact gaatggcatc
      901 gatcacaagt taaataaaca atttgtagtg aaaaaaattg gaaccgaaga aagagatgat
      961 tttcaagatg ctggaattga atattacgaa tatcttaatt gattaatcag tcagattgga
     1021 tttaacaagc atgacaaaat ccacttcaat catccaagaa atgaatcaat ttcaaaatcc
     1081 gatccgttga ctagtgtcaa tttattgtat agtcgaaaca tgaaaaactg caccacctta
     1141 ctgtttgtta cagcgttcat cctgactagc tgtcaggctg actacaacaa acctgtagag
     1201 attgcggctg ttgatgcaga gcgaatcatc aacgcagaca gagagccgca gaattggctc
     1261 gcacatggtc gcacctacga tgagcagcga ttcagtccac tcactactat caatgcagat
     1321 agcatcagca aacttgggct ggcctggtac ctagatctag gtaccaaccg tggtcttgag
     1381 gctacaccta tcgttgttga tggtgttatt tatgtcagtg gtgcatggaa tattatttat
     1441 gccatcgatg cacgatcagg gaaactctta tggcaacatg atccagagac ttcgcgggcc
     1501 tgggtggctg agtacgctgt tcatgatgcc gtcagccgag ggatagctgt atgggacggt
     1561 aaggttattg cagccacact tgatggtcga ctgttagcgc ttgatgccac aaacggtgaa
     1621 cttctctggt cagtattaac tattgatcgc agtaagccat acaccattac aggggcacca
     1681 cggattgtaa aagaccgggt aattatcggt aactctggtg ctgactatgg cgtacgtggt
     1741 tacttgtctg cttatgatgt aagtaatggt gagcttgact ggcgttttta tacagtaccc
     1801 ggtgatcccc tgaagccaca ggaaagtgca gtactcgaga gagtggtaaa aacatggacg
     1861 ggtgacgaat ggtacaagac tggtggaggc ggaacggtct ggaactcaat ggccttcgat
     1921 ccagagatga acctgttgta ctttggtgtt ggtaacggca caccatggag tcgggaatta
     1981 cgcagccctg gcggtggaga caacttattt ttatcgtcca ttgtggcggt taacccggat
     2041 aacggtgagt atgtatggca ttatcagacg acaccaggag agtcttggaa tttctctgcg
     2101 gtggaatcaa taatccttac tgaacttgag attgagggca agctacgcaa ggttttgatt
     2161 caggcaccta aaaacggttt cttttatgtg ctcgatcgcg ccactggcga actactttca
     2221 gctcagcaat acatcgaaca aaactgggcc agtcatgtag acatggctac cgggcggcca
     2281 gtggaatatc catttgcacg ttacaaaaac cagggtcgct taatcgtgcc aggtccctca
     2341 ggcggacata gttggtatcc aatgagctac agtccacaca caaagctggc atatattcct
     2401 gctctgagtg tgccgggtta ttacaatcac caggaagcat atgcccatca gcctggtttt
     2461 caaaataaag gtatcgaaga gggtgtttcc gatttccgcg cactcaatcc gcagggtgag
     2521 cggcaaaaaa ttcgctttgg tatgcgattg ttagcttggg atccacaaac ccagacagag
     2581 cgctggcggg ttgaacacac agatctacct ggtggcacgt tggccatgac cggtaacctg
     2641 gtatttcaag gtttggggag tggtgagttt gcagcgtatg ctgctgacac gggtaccaag
     2701 ctgtggagct ttgaggccgg cacgactatc atggcgggac cagtcaccta tgcagtagat
     2761 ggggaacaat atgtggcagt gttggcaggt cgtggtggtg gtgctgggct tgttggcgga
     2821 ccaacgggcg tacactggaa tggtttggtt aacgaaaatc gcttgcttgc atttaagctt
     2881 ggaggactta ccaaaattcc gtctgcacga aaactagttc gacatgtgag tatcccggat
     2941 gagttgcgat ctacaacaga agataaggca cttattggtc gcggacagaa aatctacgag
     3001 caatactgct atatgtgtca tggtctcggt gtgttttcag gcggtgtgat ccctgattta
     3061 cgctatgcga actcgcagac gtttgcgaac tgggaagcaa tcgtaattgg tggtagccgt
     3121 tctgcccaag gcatgcgctc atttgagggc gcattaagtg cagaagatgc actggcgatc
     3181 cgtgcttatg tactgcggca agctgtaaaa ttacaatgaa aggagcagtg cccatatttt
     3241 ggaataaact aatatgaaaa aatctatatc aatcattcaa gaaatgaatc aatttcaaaa
     3301 gctgatgcaa gaatggcgtc gagatataca tcaacaccca gaattagcat ttaatgagaa
     3361 tcgaactgca gagttggtgg ctaaaaccct agaatctttt ggagttgagg tatttactgg
     3421 aatagctaaa acaggagtag ttggcaaact aagtagtgga aactcagaca atgctattgg
     3481 tctcagggcc gatatggatg cattacctat ggatgaggac aatacctttg actatcgttc
     3541 ccaaaatgat ggtgttttcc atggttgcgg tcacgatggc catacagcaa tgttgctggg
     3601 tgcagcaaaa tatttatctt caacaaaaga gtttgatgga acaatttatt tcatttttca
     3661 accagcagaa gaagcagcag gcggggcacc tgaaatgatc aaggacggac tttttgagaa
     3721 ttataaaatg tcttctgtat acggaatgca taacatgcca ggaatgcctg ttggaatgtt
     3781 tggtattcgc tcaggccata tgtttgcagc atttgctcca tttgatatag aaataagagg
     3841 aattg
//
LOCUS       DASCTH010000130        10943 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000177952, whole genome shotgun sequence.
ACCESSION   DASCTH010000130 DASCTH010000000
VERSION     DASCTH010000130.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000177952"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="QGH35_02735"
     CDS             <1..1050
                     /locus_tag="QGH35_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81483.1"
                     /translation="YGTTEYFIYGSYIYYSYANEEALFGQIDYDLGDWTVSVGLRSYD
                     LEDGYKTSEYGMFYIDYDTYDTTGEGCDGDEPTGVTCNVEGGKEDGTRPKVTLSYEVN
                     DDLTLFAVSSAGYRQGGNNAALPYYCIAETVGFKRRYTSDEAKNKEIGIKSRGGNFNI
                     NATFFWVDWQDIQVVIRPACGWSFTYNGGEAETKGLEIDGSFNINDNLTLDFAGSFIS
                     AKITKDIASLGATAGDRLPNVTEDQFSIGLSYTFNLFSKPTFARFDLNYYGDAFATFA
                     EDPADMSPSYSQLNFNIGMAIDDTSKLQLSVSNLTDNRSEAFRFSTEAPSYRARNYLQ
                     WIPPRTVSVAYSKSF"
     gene            complement(1096..2358)
                     /locus_tag="QGH35_02740"
     CDS             complement(1096..2358)
                     /locus_tag="QGH35_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019529744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81484.1"
                     /translation="MRKLPLVLALFIQFTITDLFASSIILQPKNILDVDDGTLYQSQI
                     LIENGIIVEIGKNLSKRNNNIELLELAELTLVPGLMDSHVHLTGNTELKGYESIGESS
                     YLSTIYGVINAKKTLLSGFTTVRNVGAGNFADVALKQAINRNAILGPTMFVSGPALGI
                     TGGHCDSNILPFDYKYTSQGVADGPWEVRKKVRLNRKYGADLIKFCGTGGVMSKNTDV
                     NAKQYTLEEMQAIVDEAHNHGMKVATHAHGLIGIKTAIIAGVDSIEHASFIDDETIEL
                     AKKRGTFLSMDIYVSDYILGEGAAKGILEESLEKERNVGRYQRQNFQKAVKANAKITF
                     GTDAGIYPHGTNARQFKYMVEWGMTPLQAIQAATINTAELFGLSNTGMIKKNYEADII
                     GVKGNPLKDITLLENVSFVMKDGIVVKP"
     gene            complement(2446..3384)
                     /locus_tag="QGH35_02745"
     CDS             complement(2446..3384)
                     /locus_tag="QGH35_02745"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HJL81485.1"
                     /translation="MRILFLIIGVLLTASCSTQNLNNPRNENLVNTGKEYLSSKNYDT
                     RGNSQVRFLVLHYTSSNWQRSLQLLRLPEYEVSSHYLIPETNDDSYRKSELDIYQLVD
                     ETDRAWHAGKSRWEDGENINDQSIGIELVNRSKCYYQENNDKLDYKNDYLCIFPDFDP
                     LQIQLLIKLTQDILQRYPAITPTRIIGHADIQPKNKSDPGPQFPWFTLYNNGIGAWYD
                     NETVHKFWLRYSQQPIPSPLVVQCAFGIYGYGIEVTGVHDEQTFDIIRAFQMHFTPWQ
                     TNGEIDIVTVSTLWALLEKYFPESFDEKGELFCRNN"
     gene            complement(3394..3780)
                     /locus_tag="QGH35_02750"
     CDS             complement(3394..3780)
                     /locus_tag="QGH35_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012790.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HJL81486.1"
                     /translation="MRKLFITLMILLLCHNSLMAHVESIDVLELQRKLESSTETIFLL
                     DVRSKQEYQSGHIKNSINIPHDVLIKDINLIGEYRNEPLVVYCRSGVRAQLVIDKLLD
                     NDFNHIIDINGDMLAWIESDLPIEKD"
     gene            complement(3793..4434)
                     /locus_tag="QGH35_02755"
     CDS             complement(3793..4434)
                     /locus_tag="QGH35_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HJL81487.1"
                     /translation="MSQIFLIISILIPVIFAITFHEVAHGWVAKQYGDRTAEMQGRLT
                     LNPIAHIDPIGTLLLPGLLLYLGGLVFGWAKPVPINPVNLNNPKKDMFFVAIAGPIAN
                     LMMTIFWVILLYLFKSIFNDGNSSIMIFLNTMCETGIFINLILMIFNLIPVPPLDGGR
                     ILRSIVNNQFGRMLDSIEPFGLFIVIGMLFIGLLEPIFLFVRTLTITLSSIGF"
     gene            complement(4445..5710)
                     /locus_tag="QGH35_02760"
     CDS             complement(4445..5710)
                     /locus_tag="QGH35_02760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010159583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HJL81488.1"
                     /translation="MNSSKPFDIQDVNLITLAIPIFFILILCEYLYGVIAKNNTYRLN
                     DAFSSISLGMISRLPVILNLGILWGLVYNFIVKDFNLGIFQNDHWTTWAVAFLLYDLS
                     YYWMHRLSHEIKILWASHVVHHQSEEYNLSTALRQTGTGFLFTSIFYIPMILLGIQLK
                     VFITVAAINLIYQFWVHTEHIKKMGLIELIFVTPSHHRVHHAQNPEYVDTNHGGVFII
                     WDKLFGTFVKERSDLKPIYGTVQPLRSWNPIWANFEIFHQMFKDAIYTKKITDKIKLW
                     FSATGWRPDDVVKRFPSQVNDLTAFKKYDPAITKEIRIFAFLQFLIINVISTAIIFTL
                     NEQTYAEKLVFAIILILSVTSISMLLENIKGSYGLEFARSIVVVVLFFKNILEPDLLA
                     SQIILVQGLFNIIYITFLIPIRRQIQMND"
     gene            complement(5736..6755)
                     /locus_tag="QGH35_02765"
     CDS             complement(5736..6755)
                     /locus_tag="QGH35_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81489.1"
                     /translation="MKKLISLAIFILVQNNGYAINIQQAYQIPNIAESAEAYYAPDSI
                     HIIAQVQDETAQEPGPGKAGGALTYIFTDQGTDIFRVNDHGQDACSFFFPDMSKIVWT
                     STRDNMDMPLGNWSDDSDYPQGSELYISNLDGSNIKRLTNNEYYEAEVSVSPDGEWIV
                     FGRQIQGNMDLWKMRSDGSEEQQITFTEDLQEGAPFYLPDSKTILYRAWRNSEKNIHP
                     TPMTVFTIHHDGTHRTQRTFDDDMNWAPYPAPDGKHYVFVRIVEGFNWEIFLGDLSGG
                     EPTRLTYNPSFDGFPALSPDGTKLIFSRSTGERFMAGLYTFVMDVSSLNLGPENYQGI
                     PDYSP"
     gene            complement(6776..7948)
                     /gene="ubiM"
                     /locus_tag="QGH35_02770"
     CDS             complement(6776..7948)
                     /gene="ubiM"
                     /locus_tag="QGH35_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HJL81490.1"
                     /translation="MKYDIAVMGGGPAGLSFAQALSDTTLKIAVIEKQSSKVLSMPPD
                     DGREIALTHHSKKILVNLGIWDVIDKKIISLIKEAKVLNGTSPYSLHFNHQDTIENTL
                     GYLVPNNLIRAAAYKKVKESSNIKVMSDVVVESFTTDMNSVQLQLSNQKKLEADLLIA
                     ADGRLTNTRRQMGIPTEVKDFGKTCIVCKMKHDKPHNHLAYECFHYGRTLAVLPMAGH
                     YSSIVITISSEKAESIMQMDDQDFSQDIASRFNHRFGEMQLEGERHAYPLIAVHANYF
                     HSTRFALIGDASVGMHPVTAHGFNLGLRGAQVLADLIKDALSRNLDIASVNVLKQYNQ
                     KHQRNTRPLYHGTNLIVDLYNSNKISAKVIRRTALRFGNRFTPIKKIIMNQLTEAH"
     gene            8068..8529
                     /locus_tag="QGH35_02775"
     CDS             8068..8529
                     /locus_tag="QGH35_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HJL81491.1"
                     /translation="MNIKKTIEPYIEIQGGLIPALHAIQNDAGYLDIQSIKTIADGFN
                     ISTAEVIDVITYYDDFRQEKAGQFIIKVCQAESCQSLDSRGLLNSLKSHFDLEIDTTA
                     KDKSVTLKEVFCLGNCALSPSVSINDSVIGRATTDKVLLHLKDCKDNLDND"
     gene            8522..10042
                     /locus_tag="QGH35_02780"
     CDS             8522..10042
                     /locus_tag="QGH35_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HJL81492.1"
                     /translation="MTDLTRIFIPQETSACSVGANSIASVITTLATENNEIEIIRNGS
                     WGAFWLEPFIEIESQNKRRGLSYVDKDVSQFSSIEEFLTDFHDNEPFDIQELDFIKNQ
                     NRLVFSRIGYCNPLNLDEYIKFKGYKGLERAFEIGQTDTINQIRLSGLTGRGGAAFPT
                     GIKLQTVHDQESEVKYIVCNADEGDGGTFADRLIMESDPFSVIEGMTIAAYSIGATKG
                     YIYLRSEYPLAKYFLSQGIQIARSRNLLGKSILGTSFDFDIELRIGAGSYVCGEETAM
                     LESIEGRRGLVRSKPPLPAIEGLFKKPTLINNVITLSTMTTILAEGCEIFSEHGAGKS
                     VGTMPFQLSGNIKQGGLIETPFSITLEELIYDFGQGTLNNKSIQAVQIGGPLGTYLPV
                     NLFNTALTFEALHELNGSLGHGGVIVFDETTDMILQAKFAMEFCQKESCGKCTPCRIG
                     SGIGMSLIDKLNKRNAGISDRVLLEELCETMEISSLCAMGSMTPNPVRSIMKHFLK"
     gene            10052..>10943
                     /locus_tag="QGH35_02785"
     CDS             10052..>10943
                     /locus_tag="QGH35_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020406447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HJL81493.1"
                     /translation="MTTPKNIDHGTPVVQSENMISAKVDGMEIRVPEGTSIMRAASLV
                     GIQIPKLCASDNLHAFGSCRLCIVDYKNRKETGYPSSCTTPIVKGMEVTTHNEKLHYL
                     RKNILEMYISEHPLDCNSCSANGDCDLQEQAKNHDLLLSDNTLQPSKYGSASCDLMSK
                     SIDESNPYFSFDPNKCIVCSKCIRACNEIQGTYALSLYSRSNKTQIKPGLAENFLNSE
                     CVSCGACVQTCPTGSLIEKTIIEKGVPEKSINTTCGYCGVGCSFKAEIKNNELIRMTP
                     QKDGKANHGHSCIKGRFAWGY"
BASE COUNT         3473 a         1982 c         2018 g         3470 t
ORIGIN      
        1 tacggaacta cagaatactt catctatgga agttatatct attactcata tgctaatgaa
       61 gaggcactct tcggccaaat agactatgat cttggtgatt ggaccgtatc agttggactg
      121 aggtcatatg atctggaaga cggttataaa acctcagaat atggtatgtt ctacattgat
      181 tatgatacct atgatactac tggcgaagga tgtgatggag acgagcctac cggtgtgact
      241 tgtaacgtgg aaggcggtaa agaagatggt accagaccta aagttactct cagttacgaa
      301 gttaatgatg acctgactct attcgcagtt tcttcagcag gttacagaca gggcggcaac
      361 aacgctgcat tgccttatta ctgtattgct gaaactgtcg gattcaaaag acgctacact
      421 tcagatgagg cgaaaaataa agagattggt atcaaatcca gaggcggtaa ctttaatatt
      481 aatgcaacat tcttttgggt ggattggcag gatattcaag ttgtaataag accggcttgt
      541 gggtggagct ttacctataa tggaggcgag gctgaaacca aaggtcttga gattgatggt
      601 tctttcaata tcaatgataa tttaacactt gattttgccg ggagttttat ttcagcaaag
      661 atcacgaaag acattgcatc cttgggagca actgctggtg atagattgcc taatgttaca
      721 gaagatcaat tcagtattgg actatcttat acatttaatt tgttttcaaa accaacattt
      781 gcacgctttg atttgaacta ctatggtgat gcttttgcca catttgcaga agatcctgca
      841 gatatgtcac caagttattc ccagctgaac ttcaatattg gaatggctat agatgatact
      901 tctaaattgc aactatcggt cagcaatctc accgataata gaagtgaagc ttttagattc
      961 tcaacagaag cgccgagtta cagagctagg aattatctac aatggattcc tcctagaact
     1021 gtatcggttg cctatagtaa gagcttttag ttctaaagca atactaataa aaagcccggg
     1081 tcaaccgggc tttttttatg gttttactac aataccatct ttcataacaa aggagacatt
     1141 ttctagtagt gtgatatctt tgaggggatt tcctttcaca ccaataatgt cagcttcata
     1201 gttttttttg atcattcctg tattagataa tccaaagagt tcggcagtat tgatggttgc
     1261 tgcttgaata gcttgcaagg gtgtcattcc ccattccacc atatatttaa attgtcgggc
     1321 atttgtgcca tgtggataaa tcccagcatc ggtgccaaat gtgatcttgg cattagcttt
     1381 aactgccttt tgaaagttct gtctttgata tctaccaaca tttctctctt tctcaagtga
     1441 ttcttcaagg atccccttgg cagctccttc gccaagaatg taatcagaga cataaatatc
     1501 cattgataag aacgtacctc tcttctttgc taattcaatg gtctcatcat ctatgaaact
     1561 tgcatgttct attgaatcaa caccagcaat gatggcggtt ttaataccaa tcaaaccatg
     1621 tgcatgtgta gccaccttca tgccatgatt atgtgcttca tccacaatag cctgcatttc
     1681 ttctagtgtg tattgtttag cattcacgtc agtattttta gacatgacac caccggtgcc
     1741 acaaaattta atcagatctg ctccatactt tcgatttaac cttacttttt ttctaacttc
     1801 ccatggacca tcagctacac cctgtgaagt atatttgtaa tcaaagggta gaatattgct
     1861 atcacaatga ccgccagtaa ttcctaatgc cggtcctgaa acaaacattg taggtcccaa
     1921 tatggcattg cgattaattg cttgttttaa agcaacgtcg gcaaaattcc cagcccccac
     1981 atttctaaca gtagtaaatc ctgataaaag tgtctttttt gcattaatga caccgtatat
     2041 ggttgagaga taagaagatt caccaattga ctcataacct ttgagttcgg tgttaccagt
     2101 tagatggaca tgggagtcca ttaatccagg cacgagagtt aactctgcaa gctccaagag
     2161 ctcaatatta ttgtttcttt tagataagtt ttttccaatt tcaacgatga ttccattttc
     2221 aatcagtatc tgggattggt atagagtgcc atcatcaaca tctaaaatat ttttcggttg
     2281 caaaatgatg gaacttgcga acagatcagt aattgtaaac tgaatgaaaa gtgcaagcac
     2341 taagggtagt tttctcatat tattctccgg aaaaagatca aaactagtct atttgtataa
     2401 ctgaaacatt gatataggtc aaacccatag tttgtcacta aaaaattaat tgtttcgaca
     2461 aaatagttct cccttttcat cgaatgattc gggaaaatat ttttctaata atgcccagag
     2521 ggtcgaaact gtaacaatat cgatctcgcc gttggtttgc cacggagtaa aatgcatttg
     2581 gaacgcacga atgatatcaa atgtttgctc atcatgaaca ccagtgactt caataccata
     2641 gccataaatg ccaaaagcac actggactac caaaggggaa ggtattggtt gttggctata
     2701 tcgcagccag aacttgtgga cagtttcatt gtcataccac gccccgatgc cattgttgta
     2761 taaagtaaac catggaaatt ggggtccggg atctgattta ttctttggct gaatatcagc
     2821 gtgcccgatg attcttgtgg gtgtgattgc cgggtatcgc tggagtatgt cttgggtaag
     2881 tttaatcagg agttggatct gtaatgggtc aaaatctgga aaaatacaca aatagtcatt
     2941 tttataatca agcttgtcat tgttttcctg ataataacat ttgcttctgt taaccagttc
     3001 tatgcctatc gactgatcat tgatattttc tccatcttcc cacctacttt ttcccgcatg
     3061 ccaagcacgg tctgtttcat cgactaattg ataaatatct aattctgatt ttctgtaaga
     3121 atcgtcatta gtttcaggta taagataatg tgaactgacc tcatattcgg gcaatctgag
     3181 aagctgcaat gacctttgcc aattagaaga tgtgtaatgg agtaccaaga atcttacttg
     3241 gctgttgcct ctggtatcat agtttttaga tgataagtat tctttcccag tatttactaa
     3301 attttcattt ctgggattat ttaggttttg tgttgaacaa ctagcggtta acaaaactcc
     3361 tataatcagg aacagtattc tcatacaact aaattaatcc ttctctattg gcaaatctga
     3421 ctctatccat gccaacatat ctccatttat gtctattata tgattgaagt cattatccaa
     3481 aagtttatca atcactaatt gtgctctaac accacttcta caataaacaa ccaaaggctc
     3541 atttctatac tcaccaataa gattaatatc tttgattaat acatcatggg gaatgttaat
     3601 tgaatttttt atatgacctg attgatattc ttgttttgat cttacatcca gaagaaaaat
     3661 tgtttcagtc gaactctcaa gctttctttg aagttctaaa acatcgattg attcaacatg
     3721 agccatcagt gaattatggc ataacaacaa aatcattagg gtgataaata gttttctcat
     3781 actttgaaac agttaaaagc caatactact aagagttatg gttagagttc tgacaaatag
     3841 aaatataggt tctaataaac caataaacaa catgccgatg acgataaaaa gcccaaaagg
     3901 ttctatggag tctagcattc tgccaaactg attattgaca atacttctaa gaattctccc
     3961 cccatccaga ggtggcactg gaattaaatt aaaaatcatc aagattaaat ttatgaaaat
     4021 accggtttca cacattgtat ttaaaaaaat cataatactt gaattcccat cgttaaaaat
     4081 tgatttaaac aaatacaaca aaattaccca aaatattgtc atcatcaaat tggcaattgg
     4141 tcctgcaatt gctacaaaga acatatcttt ttttggatta tttaaattca ctgggttaat
     4201 aggcacaggt ttggcccaac caaagactaa accacccaga tacaataaaa gacctggaag
     4261 taacaaggtg ccaatcggat caatatgtgc aattggattt aaggttagcc tgccttgcat
     4321 ttctgcggta cgatctccgt attgttttgc aacccaacca tgagccacct cgtggaacgt
     4381 aatggcaaag attactggaa tcaaaattga gataattaga aaaatttgag acatgttaat
     4441 ggtctcagtc gttcatttga atctgtcttc taattgggat tagaaacgta atgtaaatga
     4501 tattaaataa tccctggaca agtattattt gagaggccag aagatctggc tccaaaatat
     4561 ttttgaaaaa tagaacaact acgacaatgg aacgggcaaa ttctaaacca taagagccct
     4621 taatattctc taacaacatt gatattgaag tcactgagag aattaaaata atggcaaaaa
     4681 ctaatttttc tgcatacgtt tgttcattta aggtaaatat tattgcagtt gaaatgacat
     4741 tgattataag aaattgaaga aaagcgaata ttctgatctc tttggtaata gctggatcat
     4801 attttttaaa tgctgtcaga tcattaacct gactagggaa tctcttgacc acatcatctg
     4861 gacgccaacc tgttgcagaa aaccagagct taattttatc cgttatcttt tttgtataaa
     4921 ttgcatcttt aaacatctgg tggaatattt caaagtttgc ccaaatagga ttccaacttc
     4981 tcaatggctg cacggttcca tatataggtt ttagatcaga tcgctctttt acaaaagttc
     5041 caaataattt atcccatatg ataaaaacgc caccatgatt ggtgtcgacg tattcaggat
     5101 tctgtgcatg atgcactctg tgatgggagg gcgttacaaa tattaactct ataagaccca
     5161 tttttttaat atgttcagta tgcacccaaa attgataaat taaattgatg gcggctaccg
     5221 ttatgaatac ctttaattgg atgcctagaa gaatcattgg gatatagaaa attgatgtga
     5281 ataaaaatcc tgtaccggtt tgtcttagag cagttgatag attgtattcc tcactttgat
     5341 gatggaccac atgtgatgcc caaagaattt ttatttcatg agacagtctg tgcatccaat
     5401 aataggatag atcataaagt aagaaggcaa cggcccatgt agtccaatga tcgttttgaa
     5461 aaattcctaa attaaaatct ttcactataa agttataaac gaggccccat agaatcccta
     5521 aattcaatat aacaggtagc ctactaatca tgcctagtga aatacttgaa aatgcatcgt
     5581 ttagcctgta ggtattgttt tttgcaatta ccccatataa atattcacag agaattaaaa
     5641 tgaagaaaat gggaattgca agtgtaatta aattaacgtc ttgaatatcg aacggttttg
     5701 aactattcat ggtccatctt atctagtatt gatcattatg gtgaataatc tggaatacct
     5761 tgataatttt ctggccctag gtttagagat gaaacgtcca tgacaaaagt ataaaggcca
     5821 gccatgaatc tttcccctgt gcttctactg aaaatcaatt tcgtcccatc aggtgaaagg
     5881 gcagggaatc catcaaagct tggattatag gttaaccgag tgggctcacc ccctgagaga
     5941 tcgcctaaga atatctccca attgaaacct tcaacaattc ttacaaaaac atagtgttta
     6001 ccatccggtg ctggataggg tgcccagttc atatcgtcat caaaggttcg ttgagttctg
     6061 tgtgtcccat catggtgtat cgtaaagact gtcatcggtg ttgggtggat atttttctca
     6121 ctatttcgcc aagcacgata taggattgtt ttactatctg gcaaataaaa aggagctcct
     6181 tcttgtaaat cttctgtgaa agtaatctgc tgttcttctg atccatctga cctcatcttc
     6241 caaagatcca tatttccctg tatctgtctc ccaaaaacaa tccattcacc atctggtgaa
     6301 acagagacct ctgcttcata atattcattg tttgttaatc ttttaatatt gctaccatca
     6361 agattagata tatataactc tgatccttga ggataatcgc tgtcatcaga ccagtttcct
     6421 aatggcatat ccatgttatc tcgtgttgag gtccatacga tcttactcat atcaggaaaa
     6481 aagaatgaac aggcatcttg cccgtggtca ttgactctaa atatatctgt gccttgatct
     6541 gtaaatatat atgttaatgc accacccgct ttcccaggtc caggctcttg ggcagtctca
     6601 tcttggacct gagctataat atggatacta tcaggagcat aataggcttc cgccgactca
     6661 gctatattag gtatttgata agcctgttgt atgtttattg catagccatt attttgaact
     6721 aaaataaaga tagccaatga gatcagtttt ttcatcggat tgattttttt gttaattagt
     6781 gggcttcagt caattgattc attattattt ttttgattgg cgtaaatcta ttgccaaacc
     6841 tcaaggcagt ccttcggatc actttagctg aaattttatt actgttatag agatcaacaa
     6901 ttagattggt cccatggtat aagggcctag tgttgcgttg gtgtttttga ttgtattgtt
     6961 ttaaaacatt tactgatgca atatcaagat ttctagatag agcatcttta atgagatcag
     7021 caagtacttg tgctcctctc aagcctaaat taaaaccatg tgccgtaaca ggatgcatcc
     7081 caacggatgc atcaccaatc aaagcgaatc tggttgaatg aaagtaattt gcatgaactg
     7141 caattaatgg ataggcatgc ctttcacctt ctaactgcat ttcaccaaac ctgtgattaa
     7201 aacgggatgc tatatcttga ctaaaatctt gatcatccat ttgcatgata gattctgctt
     7261 tctcagatga gattgtgatg acaatcgatg aataatgtcc agccatcggt aagactgcta
     7321 gggtacgacc ataatgaaag cattcatatg ccaagtggtt atgaggtttg tcatgtttca
     7381 ttttgcaaac aatacatgtt ttcccaaaat ctttgacctc tgtcggtatc cccatctgtc
     7441 ttctggtatt tgttaaccga ccatctgcag caatcagtaa atctgcttcg agtttctttt
     7501 gattgctcag ctgtaattgc actgaattca tatcagtagt aaaggactca acaacaacat
     7561 cactcatgac tttaatgttg ctagattctt ttactttttt ataagccgct gccctgatca
     7621 ggttgtttgg aactaggtag cctagggtat tttcaattgt atcctgatgg ttgaaatgaa
     7681 gcgagtaggg cgatgttcca ttcaagactt ttgcttcttt aatcagcgaa ataattttct
     7741 tatcaatgac gtcccaaata cctaaattga ccaagatttt ttttgaatgg tgagttaatg
     7801 ctatctctct accatcatct ggaggcattg ataaaacttt gcttgattgt ttttcaatca
     7861 cagcaatttt caaagtagta tcggataagg cttgtgcaaa acttaaacca gcaggacctc
     7921 cacccatgac tgcaatatca tatttcataa ctagtaccca tttattctaa tgccttaacc
     7981 actgtaagtt attataatat caatatttaa ttatgataaa tatagttttc cagtgaatga
     8041 aaaataataa caacagaggt taatatattg aatatcaaaa aaactattga accctatatc
     8101 gaaattcaag gtggcttgat accagcatta catgcaattc aaaatgatgc tggttatctc
     8161 gatatccaaa gtattaaaac tatcgctgat ggctttaata tttcaactgc agaagtcata
     8221 gatgtcatta catactatga tgattttaga caagaaaaag ctgggcaatt tattatcaaa
     8281 gtatgtcagg ctgaatcctg tcagagtctt gatagtagag gtctattgaa ttctttgaaa
     8341 tcacattttg accttgagat agatacaaca gcgaaagata agtcagtgac attaaaggaa
     8401 gttttttgtc ttggtaattg tgctttaagt ccttctgtat caattaatga tagtgtcatc
     8461 ggtagagcaa cgactgataa agtccttctt cacctaaaag attgtaaaga taatctagat
     8521 aatgactgat ttaacaagaa ttttcattcc acaagagacc tcagcttgtt ctgtaggtgc
     8581 aaactccatt gccagtgtaa tcactacatt ggcaacagaa aataatgaga tagaaatcat
     8641 tagaaatggg tcatggggtg cgttttggct agaaccattt atagagattg aaagccaaaa
     8701 taaacgaagg ggcttatcct atgttgataa agatgtttct caatttagtt caattgaaga
     8761 atttttaact gattttcatg acaacgaacc ttttgatata caagagcttg atttcataaa
     8821 aaaccaaaac cgattggttt tctcaagaat tggatactgt aatccactga atcttgatga
     8881 gtatattaaa ttcaaaggtt acaaaggctt ggaaagagca tttgagattg gacaaacaga
     8941 cactatcaat caaatcagat tatctggcct tacaggtcgt ggaggtgctg cgtttccaac
     9001 aggcataaaa ttacaaactg ttcacgatca agaatctgaa gttaaatata tagtctgtaa
     9061 tgccgatgaa ggtgatgggg gaacatttgc tgatcgactc attatggagt cagatccctt
     9121 ctcggttatt gaaggaatga caatcgctgc ttatagtatt ggagcgacaa aaggatatat
     9181 ttatcttagg tctgaatacc cattggcaaa atattttcta agccaaggaa ttcaaattgc
     9241 tcggtctcga aacctgcttg gaaagagcat actgggaact agttttgatt ttgatattga
     9301 gcttagaatt ggagcaggtt cctatgtttg tggagaagag acagccatgc tggagagtat
     9361 agaaggtaga aggggcttgg ttagatcaaa acctccattg ccagctattg agggtttatt
     9421 taaaaaacct acactgatta ataacgtcat aacattatca acaatgacca caatattggc
     9481 agagggttgt gaaatttttt cagaacatgg tgctggaaaa tcagttggaa caatgccatt
     9541 tcaactcagt ggaaacataa aacaaggtgg tttaattgaa acacccttca gtattacttt
     9601 agaagaactt atttatgatt tcggccaagg aactctaaat aataaatcca ttcaagctgt
     9661 acaaattgga ggacctttgg gcacttattt gcctgttaat ttattcaata cagctttaac
     9721 ttttgaagca ctacacgagc taaatggctc attgggtcac ggtggagtaa tcgtatttga
     9781 tgaaacaacc gatatgatct tgcaagctaa atttgcaatg gaattttgtc aaaaagaatc
     9841 ttgtggcaaa tgtactccat gtagaattgg ttcaggaatt ggtatgtcat taatagataa
     9901 acttaacaaa aggaatgctg ggattagtga tagagtatta cttgaagaat tgtgtgaaac
     9961 aatggaaata tcatcattgt gtgctatggg tagtatgact ccaaatccag tgcgctctat
    10021 aatgaaacac tttcttaaat aaaaatatca aatgactact cctaaaaaca ttgatcacgg
    10081 aacaccggta gtgcaatcag agaatatgat ctcggctaaa gttgacggaa tggaaattag
    10141 agttcctgaa ggaacatcca ttatgagagc ggcctcactt gttggaattc aaattcctaa
    10201 attatgtgca tcagacaatt tacatgcatt tggttcgtgc cgcctttgca ttgttgacta
    10261 caagaaccgt aaagaaactg gatacccttc ctcctgtaca acaccaattg taaaaggtat
    10321 ggaagtcacc acacataatg aaaaactaca ttatttgaga aaaaatatac tggaaatgta
    10381 tatctcagaa catcctctag attgtaactc atgttctgca aatggtgatt gtgatcttca
    10441 agagcaagcc aaaaatcatg atcttttatt atcagataat acccttcagc caagtaaata
    10501 tggttctgcc tcttgtgacc taatgtcaaa gagcattgat gaatcaaatc catatttcag
    10561 ttttgaccct aataaatgca tcgtttgctc gaaatgtata agggcatgta acgaaattca
    10621 gggaacctat gctctttcac tttactcacg cagcaataaa actcaaatta aaccaggact
    10681 tgcagaaaat tttttgaatt ctgaatgtgt atcgtgtggt gcttgtgtac aaacatgtcc
    10741 aacaggaagc ttaatagaga aaactattat tgaaaaagga gtgcctgaaa aatcaataaa
    10801 tactacttgt ggttactgtg gagtgggttg ttcatttaaa gctgaaataa aaaataatga
    10861 gctcataaga atgactcccc aaaaagatgg caaagcaaat catgggcaca gctgtattaa
    10921 aggcaggttt gcttggggct ata
//
LOCUS       DASCTH010000131         1792 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000179103, whole genome shotgun sequence.
ACCESSION   DASCTH010000131 DASCTH010000000
VERSION     DASCTH010000131.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000179103"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="QGH35_02790"
     CDS             complement(<1..1571)
                     /locus_tag="QGH35_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81494.1"
                     /translation="MTDNNLKSNLKLNPVHLAVLAALYPSYQAMAQDLALEEIVVTAT
                     KRSLNIQDIASTVQAIPEESLKLMGAKSMEDFARFMPGVNVVSGVTSSTVVFRGAITG
                     SGYIASSSGSVYLDELPLTQVGSQPNVRLVDIARVEALSGPQGTLYGSDAQAGTMRII
                     TNKPVMGETSSVFDGEYRMNPEGDPSYRGSFVFNFPLKDNLALRMSLFNDEDGGYIDN
                     IYGHTADNSFGDPWPQAGKAGGHGTLDNAASVDSDVNFARVKGGRVALKWEMDDSWSA
                     TVSSMHQETSQGSGNYYDIYRGDLNLVSFHDDWYKEDWQAHAFNVEGDLGFAQLVASV
                     SYHERQGGGVWDITNYAHYWSHNYCSDSYYTQDYAPYYYANPETGYISWYPVYCQGTG
                     IDSDFYSSYQGRSGNDKLAAEIRLSGQSDRFDWLVGMYYEDAEDWWTAPFAIPTNGGK
                     VKLGESTFQSSFAAAAYRFAGIDYPSATSHWYSESATEWDQKAIFGELTWHANDKIDV
                     TFGARSFDRTNTQWY"
BASE COUNT          523 a          412 c          350 g          507 t
ORIGIN      
        1 atataccatt gggtattggt gcgatcaaaa ctccgtgcac caaaagtgac gtcaatctta
       61 tcattcgcat gccaagtcaa ttcaccaaat atagcttttt gatcccactc agttgcactc
      121 tcggaatacc aatgcgaggt tgcacttggg tagtctattc cagcaaatct ataggcagct
      181 gctgcgaatg aagactgaaa agtggattct cccaatttaa ccttgccgcc attggtgggg
      241 attgcaaacg gtgctgtcca ccaatcttcc gcatcttcat agtacattcc cacgagccag
      301 tcaaatctat cgctctggcc gctgagacga atttctgcag ccaatttatc gttaccgctc
      361 ctaccttgat atgaggaata aaaatcagaa tcaatgcccg tgccctgaca atagacagga
      421 taccaagaga tgtatccggt ttcagggttt gcataataat atggggcata gtcttgggtg
      481 tagtacgaat cagaacaata gttgtgtgac cagtaatgag cgtaattggt aatgtcccac
      541 acaccacctc cttgtctttc atgataagac actgatgcta ccagttgggc aaaaccaaga
      601 tcgccttcaa cattaaacgc atgggcctgc caatcttcct tgtaccaatc atcatgaaag
      661 gaaacaaggt ttagatcacc cctatatata tcatagtagt tgcctgatcc ttgtgaggtc
      721 tcctgatgca ttgaagagac ggtagctgac cagctgtcat ccatctccca tttgagtgca
      781 accctgccac ctttgaccct tgcaaaatta acatcgctat caacagaagc agcattatcc
      841 agtgttccat gcccaccggc tttgcctgct tgaggccatg gatctccgaa actgttatcg
      901 gcagtgtgtc cataaatatt gtctatataa ccaccgtctt catcattgaa aagtgacatc
      961 cgtaaagcaa gattgtcttt aaggggaaag ttaaagacaa aggacccgcg ataactagga
     1021 tcgccttctg gattcattct gtactcacca tcaaaaacgg aactcgtttc tcccatcacc
     1081 ggtttattgg tgatgatgcg catggttcca gcctgtgcat ccgaaccgta gagagttcct
     1141 tgtggtccag acaatgcttc aacacgtgcg atgtcaacca aacgcacatt cggctgtgag
     1201 cctacttgtg ttagtggtag ttcatccaaa tagactgagc ctgatgagga agcaatgtaa
     1261 ccagaaccgg tgatggctcc acgaaatacc acggtactac tggtaacccc agagaccaca
     1321 ttaactcctg gcataaaacg ggcaaaatcc tccatggatt tagcacccat taatttcaag
     1381 ctttcctctg gaatagcttg cacagttgag gcaatatctt gaatatttaa actacgtttt
     1441 gtcgcagtaa caacaatctc ttccagtgct agatcttgag ccattgcttg atagctggga
     1501 tatagagctg ccagtacagc aagatggact ggatttagtt ttagatttga cttcaaatta
     1561 ttatctgtca ttggtttccc ccaaagttta gatcaactat taaagttgta ttagaaccac
     1621 catacaatat agctttttta tagctttaac aatagctaaa tcgattttca gaaggtcttt
     1681 ttttcacata gatttttgta ttttcattca atatattgaa taagttaaca ctctaatgtt
     1741 gagcatccag cattgagtta aaagaaaaag caaaaaataa tcaatcaatg at
//
LOCUS       DASCTH010000132         5534 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000180557, whole genome shotgun sequence.
ACCESSION   DASCTH010000132 DASCTH010000000
VERSION     DASCTH010000132.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000180557"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="QGH35_02795"
     CDS             <1..537
                     /locus_tag="QGH35_02795"
                     /inference="COORDINATES: protein motif:HMM:NF019284.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HJL81495.1"
                     /translation="NYKDGLKDGLVEIFHWEKGQLIRRENWKQGREDGVWEEFNKETG
                     QLTNRGNFVDGKQQDFEWFNETGQLEIRGYFKDDEQDGLWEYFAENGQIAYQGTFKNG
                     KEDGLWEGYYENGQLLYTGNMKNGEADGLFEWFHENGQLQRRGEHKNGKLDGLWEYFD
                     RDGSLTETIEFKNGLKKR"
     gene            865..1740
                     /locus_tag="QGH35_02800"
     CDS             865..1740
                     /locus_tag="QGH35_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007235519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81496.1"
                     /translation="MSIDLKVVMKNKAINQWRLFWLISIPMSIVIVIAMIGADMSTGA
                     GVSTMIGFSVRWAVPFIFLVVAASSLQTLFPGPFPMWLLRNRKYIGICFAVAMAWQGL
                     FIFIMSYFFREYYFADVYFFRDELEGSIGYIFLPAMVLTSFEFGRKHLSSKQWKLLHK
                     SGIYFLWAYPFSTYWWSFSYYYGNPEPLDYVYYSFGFLAFALRIAAWGKQRQQAIQSG
                     TPVVFKVLGSAIIAFGLFVFASGRYWREPVTAFLTTPKWSADLELWLPFWPYEPFLSL
                     FIIGLGTMLVTKNRA"
     gene            complement(1818..3845)
                     /locus_tag="QGH35_02805"
     CDS             complement(1818..3845)
                     /locus_tag="QGH35_02805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HJL81497.1"
                     /translation="MKGLSMEIFDRNKKPFYLSVVFLSLFLAHGSFALQSQHEKIKLE
                     DINQIKDIGSIDVSKDADLIAYTVSSVDQKNDEYVTDIWMLNFKKDRDKLIVRSAYSP
                     KFSPDSNYVAYLAPGRGKYSDYQQLWFFNTNTSTKRQITKIESSITDFEWAPDGSRIV
                     VVINIEPDREDEGETQKPYVIDRYQFKQDYNDFLDESRQHLFLIEIATRELTQLTDGS
                     SNELFPSWSPDGDRIAYVTKIGDIDRHNNWDIYVLKLDSNENPIQLTTHLGADSQGSR
                     PQWSPDGKQIAYLYGGDPKLLWYALTELAIIDLDTHTSSIITKQLDRNTSAPQWSEDG
                     SSIFFVLEDNMKSQLAKYSFQDQKINTITSTNYYLSGWSKNYAVRNGKIGLILSDLNT
                     PDEIFFFDGKNLQQKSHQNSEFLSKKIISPTETISFKTDDGEEIFGMMVKPHDFDPSK
                     KYPLIIRMHGGPVGQYGLHFRYDWQLFAANDYIVMAMNPRGSSGRGLDFQKIIFADWG
                     NVDADDVSALADYAISLGYIDPQRLGLGGWSYGGMLTNYVIAKDSRFKAATSGAGISN
                     LLSGFGDDHYIREYILELGTPWDHTEDWLRVSNPFFNAGNIKTPTLFLVGEKDFNVPL
                     IGSEQMYQALKQLNIPTQLIIYPDENHSFSTPSYNEDVLKRYLDWYSKYLQ"
     gene            complement(3852..5366)
                     /locus_tag="QGH35_02810"
     CDS             complement(3852..5366)
                     /locus_tag="QGH35_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HJL81498.1"
                     /translation="MQENKTDKLSFSSILVAIICITLPIALKPRESEVFIRQIYLETV
                     NIFGSSYMVLGIITLVFLLILSLSKYGTFILGGENAKPEFKNFSWGSMLFCSGIGGGI
                     LYWSGVEWAYYVSQPPFGIEPFSEEAYHLSSAYGIFHWGLGGWALYCLPAIAIAVPFY
                     HFRLGSLRLSSGIRSSQQNPIENSIIGRIIDFIFVIVVIGASGGTMGMYIPVISAGIA
                     ELFDVPHDVMLDLGMLFVCTCLFAFSVFKGIKKGIRVISNINVVMALIFLMCILLLGS
                     FQEIINLTIEAIKSLIKNFSSMSTLGIKEKSNFADNWTIFYWAWWIALGPQVGLFIAR
                     VSKGRTLRELILGMLFLGSLGCFLFFAIIGNYAINLEINNQLMVTEILANEGHRIAAT
                     QVLLSLPFGEVFLFTYCIIVIIFIATSYDSVSYLLAYHVKRTSSEVNEPSKKMRLFWA
                     VVLAILPASLFLVNSHRAAMDVILLMSPPLLILFPIFAISIIRTLKNHYSDSNQ"
BASE COUNT         1732 a         1032 c         1105 g         1665 t
ORIGIN      
        1 aattacaaag acggacttaa agatggattg gtagaaatct tccactggga gaaaggtcaa
       61 ttaataagaa gagagaattg gaagcaaggg agagaagacg gtgtttggga agaatttaac
      121 aaagaaactg gtcaattaac aaatagagga aattttgtag atggaaaaca acaagatttc
      181 gagtggttta atgaaactgg tcaattagaa attagagggt actttaaaga cgacgaacaa
      241 gacggtctct gggaatattt tgctgaaaac ggtcaaatag catatcaagg aacattcaag
      301 aacggtaaag aagatggtct ttgggagggt tattacgaaa acggtcaatt attgtataca
      361 ggaaacatga aaaatggaga agcagacggt ctttttgagt ggttccatga aaacggtcaa
      421 ctacaaagaa gaggagagca taaaaacgga aaactagacg gtctctggga gtattttgat
      481 agagacggaa gtctaactga aaccatagaa ttcaaaaacg gattaaaaaa acgataaacg
      541 tttattcatt ctaattatta ttgcttacgt ttggcaaaat gcgactggat gagaaatgat
      601 aaccccattc caacaactaa aatcgcaacg gatatctttc cacgatgagc ggtttcactt
      661 gtccagattt caccgtattc aaaccagtaa agtacataca tagaaatcag ccaaggcgta
      721 aaggcaatag cgatttcaag tacgtgacga gtccgtattt tcaaggaatt taacatgtta
      781 aaaaaatact accatgaaat gtagccatcg tatacgatga gacgatgagt tcaagttctc
      841 tgggtaagca aatgatagga atatatgagt attgacctga aggtagtaat gaaaaacaaa
      901 gccattaacc agtggagatt gttctggctt atctctattc caatgtccat tgttatagtc
      961 atcgccatga tcggggccga tatgtccacc ggagctggtg tatcgacaat gatcggcttt
     1021 tccgtacgct gggcagtgcc tttcatattc ctagtagtcg ctgcatcttc tttgcagaca
     1081 ctgtttcctg ggccgtttcc aatgtggttg ctgcgtaacc gtaaatatat tgggatatgc
     1141 tttgcagtcg caatggcgtg gcaaggcttg ttcattttta taatgtcgta ttttttccgg
     1201 gagtactact tcgcagacgt ctattttttc cgcgatgagc ttgaagggag catcggctat
     1261 atcttccttc cagccatggt actgacatca tttgaatttg gccgcaagca cttaagttca
     1321 aagcagtgga agttgctgca caagagcggt atctatttcc tctgggccta tccctttagc
     1381 acctattggt ggagcttttc ctactactat ggaaatccgg aaccgcttga ctatgtatac
     1441 tactcgtttg gattcctcgc attcgcactg agaattgcgg cctggggcaa gcagcgtcag
     1501 caagcgatac aaagcggtac gcctgtcgta ttcaaggtat tgggcagcgc cattatcgcc
     1561 ttcggtcttt ttgtattcgc ttccggtcgt tattggcgag agcctgttac tgcttttctg
     1621 accacaccga agtggtctgc ggatcttgaa ctgtggctgc cgttttggcc ttatgaacct
     1681 tttttatcac tcttcatcat cgggctgggg accatgctgg taaccaagaa tagggcttaa
     1741 tgtggatgga ttataggaaa gaataatact ttaactaatc ctaaaatcat tcaatcccga
     1801 tcttgagttc tagcagatca ctgtaagtat ttgctatacc aatccagata tcttttaaga
     1861 acatcttcgt tatagctggg agtggaaaag gagtgatttt catcaggata aataattaat
     1921 tgtgtgggaa tgtttaattg ttttaaggct tggtacattt gttcagagcc aatcaatgga
     1981 acgttgaagt ctttttcacc cacaagaaat aaggttggag ttttaatatt tcctgcatta
     2041 aaaaaaggat tggaaacccg aagccagtcc tcggtatgat cccaaggggt tcctaattca
     2101 agaatgtatt ctctgatata gtgatcatct ccaaaaccac tcaataagtt actgatgccc
     2161 gcaccagaag tagcagcctt gaacctagaa tcttttgcaa tcacatagtt tgttaacatc
     2221 ccgccataac tccaaccccc aagacccaat ctttgcgggt caatatagcc tagtgatatc
     2281 gcataatctg ctaatgcact gacgtcatca gcatcaacat ttccccaatc ggcaaaaatt
     2341 attttttgga aatccaagcc tctgcctgaa ctaccccttg gattcatagc catgactatg
     2401 tagtcattgg ctgcaaagag ttgccagtca taacgaaaat gcaagccata ttgaccaaca
     2461 ggaccgccat gcattctaat aataagtggg tatttttttg aaggatcaaa atcatgaggt
     2521 ttgaccatca ttccaaatat ttcttcccca tcatcagttt taaatgaaat cgtttcagtt
     2581 ggtgagataa tttttttcga taaaaattct gaattctgat gagacttttg ttggagattc
     2641 ttcccatcaa agaaaaatat ttcatcaggt gtatttaaat ccgaaagaat taatccaatt
     2701 ttcccatttc tgaccgcata gttcttagac caaccagaaa gataataatt ggtagaggta
     2761 atagtattga tcttttgatc ttggaaggaa tattttgcca gttgactttt catattatct
     2821 tcaagtacaa aaaatattga tgagccatct tcagaccatt gaggagcaga tgtatttcga
     2881 tcaagttgtt tggtaattat tgaactagta tgagtgtcta aatctataat tgctagttct
     2941 gttagagcat accaaaggag ttttggatca cctccataaa gataggcaat ctgtttccca
     3001 tctggactcc attgagggcg tgagccctgt gaatctgctc caaggtgagt cgttaattga
     3061 atagggtttt catttgaatc tagtttgagt acataaatat cccaattgtt gtgacggtca
     3121 atatcaccga tttttgtgac ataggcaatg cgatctccat cgggagacca agatggaaat
     3181 agttcattac tggagccatc tgtgagttga gttagttctc tagtggctat ttcaattaag
     3241 aacaaatgct gtctcgattc atccaggaag tcattataat cttgtttgaa ttgataccga
     3301 tctattacat agggtttttg ggtttctccc tcatcctcac gatcaggttc tatattaatg
     3361 accacaacaa ttctactgcc atctggagcc cattcgaaat cggtaatact tgattcgatc
     3421 tttgttattt gtctttttgt cgaagtatta gtattgaaaa accagagttg ttggtagtct
     3481 gagtattttc ctctgccggg agcaaggtag gcaacatagt tgctgtcggg actaaattta
     3541 ggggaatatg cggatcgcac aatcaattta tccctatctt ttttgaaatt gagcatccag
     3601 atatcggtaa catattcatc gttcttctga tcaacgctcg atactgtata ggcaattaag
     3661 tcagcatctt ttgaaacatc aatcgatcct atatccttga tctgattgat gtcttctaac
     3721 ttaatctttt catgctgact ttgtagtgca aaagacccat gggctaagaa aagcgaaaga
     3781 aatactaccg ataggtagaa tggttttttg tttcggtcaa aaatttccat agagagccct
     3841 ttcattttaa tttattgatt gctgtcagaa taatggtttt taagtgttct gattatagat
     3901 atagcaaaaa ttgggaatag aataagcagt ggtggagaca tcagtagaat aacatccatt
     3961 gctgcacgat gactattaac caaaaacaaa gaggctggaa gtattgctag aacaacggcc
     4021 caaaacagtc tcattttttt gctgggttca ttgacttcac tcgaggttct tttgacatgg
     4081 taggcaagta ggtaggaaac agaatcataa gatgtggcaa taaatattat tacaatgatg
     4141 caataggtga aaagaaatac ttcaccaaat ggtaacgata gtaagacttg agttgctgca
     4201 attctgtgcc cttcatttgc caaaatttca gttaccatca gttggttatt gatctcaaga
     4261 ttgattgcgt agttgcctat tattgcaaag aataagaagc atcctaagct acccagaaaa
     4321 agcataccta aaatcagttc ccttagcgtt cttccttttg agactcttgc aatgaataaa
     4381 cctacttggg ggcccaatgc aatccaccaa gcccaataga aaattgtcca attatcagca
     4441 aagtttgatt tctctttaat cccgagtgta ctcatacttg agaaattttt aatcagtgat
     4501 ttgatggctt caattgtaag attaataatt tcctgaaaag agcccaaaag taggatacac
     4561 attagaaata ttaaggccat gacgacattg atattactga ttacacggat gccctttttt
     4621 atacctttaa agacgctgaa tgcaaacagg catgtgcata caaaaagcat acctaaatca
     4681 agcattacgt catggggaac atcaaacaat tctgctatac cagcactgat aaccgggata
     4741 tacataccca ttgttcctcc ggatgctcca atgacaacaa tgacaaagat gaaatcaata
     4801 atgcgaccaa tgattgagtt ttctattgga ttttgttggg atgatcttat tccagaactg
     4861 agtctcaaag atcctaatct aaaatgataa aaaggaaccg caatggcgat cgcaggaagg
     4921 cagtataaag cccaaccacc tagaccccaa tgaaatattc cataagccga agacagatgg
     4981 taagcctcct ctgagaaggg ttcaatacca aatggaggtt gactgacgta ataagcccat
     5041 tcgaccccag accaatagag aatacctcca ccaatccctg aacaaaatag cattgatccc
     5101 caactaaaat tcttaaattc tggcttggca ttttctccac ctaggataaa tgttccatac
     5161 tttgataatg aaagtatgag cagaaaaacc aaagttatga tccccaatac catatatgaa
     5221 gacccaaaga tattgacagt ctctaaataa atctgtcgaa tgaaaacttc agactctctt
     5281 ggtttgaggg ctattggtaa ggtaatgcaa ataattgcaa caaggatgct tgagaaactt
     5341 aatttatctg ttttattttc ttgcaaagag ttacctattt acctattaag aatttatcga
     5401 tgtctttgtc gtttgaacaa taactttgag tgtttctgat ttagccattt cggctgtcag
     5461 aaagaattca gcagtcatga tcaatataaa tagtataaaa aaaattaata gtgtgattat
     5521 ggtttctctc attt
//
LOCUS       DASCTH010000133         1943 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000181134, whole genome shotgun sequence.
ACCESSION   DASCTH010000133 DASCTH010000000
VERSION     DASCTH010000133.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000181134"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="QGH35_02815"
     CDS             <1..494
                     /locus_tag="QGH35_02815"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HJL81499.1"
                     /translation="YPNLSHNMKGWLTEASLLTIRLKNCAKHFELRVLNEGYRNVSSL
                     LNDLEISDRVFIREVMLLSDGVPMILGQTSIPEKTLRRHRWLSKLGNGALGEKIMGLS
                     NVQRSDFNFLFCKGNSPILKKHGIDCKTTEEDGLWMRRSSFSIQNQPLWVAEIFLPKI
                     KEL"
     gene            508..1380
                     /gene="ubiA"
                     /locus_tag="QGH35_02820"
     CDS             508..1380
                     /gene="ubiA"
                     /locus_tag="QGH35_02820"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019613757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HJL81500.1"
                     /translation="MTSVEKKLFSSANQYIQIMRLDRPIGIYLLLWPTLWGLWLASRG
                     LPDIRILVIFILGVVVMRSAGCVINDIADRDIDPLVERTKQRPLATGAIALHQAWLLF
                     LLLVVVAFMLVLQLNFFTVLLSGGGLLLTIVYPFCKRFLSAPQLVLGLAFSWSVPMAF
                     AATDQDIKLLVYVLWGSVVFWILIYDTFYAMADREDDLALNLNSTAILFGSNDKVMIG
                     LLQVAMLLCLIVVGLLFELGWYYYLSLVISCCLFLRQHYLIRNRKPKDCFQAFLENNY
                     VGGVIFVGVVLSFF"
     gene            1537..>1943
                     /locus_tag="QGH35_02825"
     CDS             1537..>1943
                     /locus_tag="QGH35_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase"
                     /protein_id="HJL81501.1"
                     /translation="MAFVIIAAVIVVGTVLFNLVTPWWFTPLASNWGSLDQTIIISLW
                     ITGVAFVLVSAFILYCVVKFRYSEDRRSSYEPENQKLELQLTVITTIGVIILLAPGLV
                     AWQSYINLPENGLQVEGVGQQWTWSFRFPGEDGV"
BASE COUNT          491 a          340 c          431 g          681 t
ORIGIN      
        1 cctatcctaa tctaagtcac aatatgaaag gctggctaac cgaagcctct ctactgacaa
       61 tcagattaaa aaactgtgct aaacattttg agttaagggt tttaaatgaa ggctatagaa
      121 acgtgtcctc actattgaat gatttagaaa tatcggacag ggtttttatt cgtgaagtta
      181 tgttgttgtc agatggtgtg cccatgattc tagggcaaac atccatccca gaaaaaactt
      241 tacgtcgcca ccgctggttg tctaagttgg gcaatggtgc gttgggagaa aaaattatgg
      301 gtctttccaa cgtccaaaga agtgatttta attttttatt ttgtaaaggc aattctccta
      361 tactaaagaa acatgggatc gattgtaaaa caactgaaga ggatggcctg tggatgagaa
      421 gatcaagttt ttcaattcaa aatcagcctt tgtgggttgc tgaaatattt ttaccaaaaa
      481 ttaaagagct ctaacacatc ttttcttatg accagtgttg aaaaaaaact atttagctct
      541 gccaatcaat acatccaaat catgcgtttg gaccgaccga ttggtatcta tctcttgttg
      601 tggcctacgc tctgggggtt atggctggcc tcgcgtggtc ttcctgatat acgcatatta
      661 gtaattttta tcttaggggt cgtggtaatg cgatcagccg gttgtgtaat caatgatatt
      721 gccgacaggg atattgatcc tttagtggaa agaacaaaac aaaggccttt agccaccggt
      781 gccattgcct tgcatcaagc ttggttgttg tttttgttgt tggtggttgt ggcttttatg
      841 ttagtcctac agctcaattt ttttaccgtt ttattgtctg gtggaggact attattaacc
      901 atagtttacc ccttttgtaa gcgtttctta tcagcgccac aattggtgct tgggctggca
      961 tttagctgga gcgttcctat ggcttttgct gcaacagacc aggatataaa gcttctcgtt
     1021 tatgtcttgt ggggatcggt ggtgttctgg attttaatat acgatacatt ttatgccatg
     1081 gcagatcggg aggacgactt ggcactcaac cttaactcga ctgctatttt gtttggctcg
     1141 aatgacaaag taatgattgg attgctgcaa gtggcgatgt tgttatgtct gatagttgtc
     1201 gggttattgt ttgagcttgg ttggtattat tacctttcat tggtcatttc ttgctgtttg
     1261 tttttaaggc agcattattt gatcagaaac cgtaagccca aggattgctt tcaagcgttt
     1321 ttggaaaata attatgtagg tggggttatt tttgtgggtg ttgtattgtc ttttttctaa
     1381 tcttatattc ttgatcacag ctgctggtca atcaatgtat tcataaattt gtctcaatcg
     1441 aggattgaat tctgtctttt tattaatcat aatgcattgc gtttatttat attttcataa
     1501 catcgtattt ctgaatttta caaaaaatag ggatttatgg cttttgtaat cattgctgct
     1561 gttattgttg taggaactgt tttatttaat ctagtcactc cgtggtggtt tactccttta
     1621 gcatcaaatt ggggcagcct ggaccaaacc attattatta gtttatggat caccggggtt
     1681 gcctttgttt tagtctctgc ttttattctc tattgcgtgg tcaaattccg gtatagcgaa
     1741 gacagaaggt cgagttacga gcctgaaaat caaaaactgg agcttcagct caccgtcatt
     1801 actacgatcg gcgttatcat actcctagca cccggactag tcgcttggca aagttatatt
     1861 aatcttccag aaaacgggct ccaagttgag ggtgtcggac aacagtggac ttggtctttc
     1921 cgctttccag gagaagatgg tgt
//
LOCUS       DASCTH010000134         6860 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000181960, whole genome shotgun sequence.
ACCESSION   DASCTH010000134 DASCTH010000000
VERSION     DASCTH010000134.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000181960"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(287..679)
                     /locus_tag="QGH35_02830"
     CDS             complement(287..679)
                     /locus_tag="QGH35_02830"
                     /inference="COORDINATES: protein motif:HMM:NF015853.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HJL81502.1"
                     /translation="MNKAICNLLFILFISISSSIAQGEFTSEQEALYQHIIQEVRCLV
                     CQNQSIAESNADLAKDLRIQIHNQINEGKTELEIKEFLLNRYGEFVLYEPSFNKKTLL
                     LWFSPLILLLLIFGWYRKIKSPLIDTRE"
     gene            complement(676..1200)
                     /locus_tag="QGH35_02835"
     CDS             complement(676..1200)
                     /locus_tag="QGH35_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HJL81503.1"
                     /translation="MIRKYLPLLIFLAFFFLLFKGLYQGQSNIPSPLIDKPMPAFNLP
                     TLGTDQLITEQDIIGQISLINIWATWCPGCLEEHEFLVQLSNSPQAIPIVGINWKDET
                     ELAQLWLKESGNPYSEVLVDSIGKTAIDFGVYGAPETFLIDEEGVIKYKHIGPLSTQI
                     WNREILPVINKMQQ"
     gene            complement(1193..3172)
                     /locus_tag="QGH35_02840"
     CDS             complement(1193..3172)
                     /locus_tag="QGH35_02840"
                     /inference="COORDINATES: protein motif:HMM:NF007691.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HJL81504.1"
                     /translation="MIPELGQLLLVIAMVAAFIQSFLGMGLTKISTDQRDRLMSRIVT
                     IHTVGLFLAMVCLGYSFVSDDFSVLYVALNSNTNLPILYKIAAIWGAHEGSFLLWLFL
                     LSMWSFLLAFSKKHNSQYLDLKITALGILGLISFSFVLFIIFTSNPFEVLPNPPIQGR
                     SLNPLLQDPALVIHPPMLYAGYVGLAVPFALALSSLMNSKKNHSQFHWASIARVWTIM
                     AWVFLTGGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLTTTALLHSLIVTERQKLF
                     NGFSILLAIISFSQSLLGTFLVRSGILISVHSFASDPSRGIFILWLLGLFTGGALLLM
                     AMKYKKEKPVKIDILSRASLLLLNNVFLCTASGLILIGTLYPLALEIFDAGKISVGAP
                     YFNFVFLIPFLPLLFFIGLGAFTSWKFSNTKIMISKLKWILVASILFGIGCTVFFYGY
                     AGFLTLIGIILAGWTILSGLMPLIERIIMFSTRLVMISSMNTLENAMKILPMSLAHIG
                     LGLTVLGITVTSSYGLTQDKSLRFGQSTQVGDYIFQLNDIKESVGPNYFSIIAEIQIS
                     SKDSDITVIYPEKRIYDRNSPAMTEAGIESNIARDLFVALGENVGDQTWSLHLQYKPL
                     LRLIWIGPFVMIIGGLFRVYLTKPLRRRKLVQQDD"
     gene            complement(3195..3614)
                     /gene="ccmE"
                     /locus_tag="QGH35_02845"
     CDS             complement(3195..3614)
                     /gene="ccmE"
                     /locus_tag="QGH35_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014590169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HJL81505.1"
                     /translation="MLPRHRRILGLIVVLLGVGLASFFVLKSFQQNLLYYFTPIQVQS
                     GEAPIDREFRMGGLVQPGSVRRAEGSVAVEFFLTDGNVFIIVKYNGILPDLFREGQGI
                     IATGRLDTEGVFIAREVLAKHDENYMPPEIHKIKETT"
     gene            complement(3790..4542)
                     /gene="ccmC"
                     /locus_tag="QGH35_02850"
     CDS             complement(3790..4542)
                     /gene="ccmC"
                     /locus_tag="QGH35_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HJL81506.1"
                     /translation="MNLRKIYKLGSPPYFFKLAILLEPWFLGCALLLISIGMYGGLFI
                     APPDYQMGDAYRIIYFHVPSAYLSMMAYVAMAMGSVVGLVWRSNVAFSFANSCAPVGA
                     SFTFLALITGMLWGKPMWGSAWVWDARLTSELILLFLYLAYILLYLSIPDRSKADRSA
                     SLFAIVGLVNIPIIHYSVVWWNTLHQASTLSRLAKPAIASEMLWPLMIMILGFSLFFF
                     GIIMRRTAAEIMNREKKTRWIGSIFSEENDNV"
     gene            complement(4558..5226)
                     /gene="ccmB"
                     /locus_tag="QGH35_02855"
     CDS             complement(4558..5226)
                     /gene="ccmB"
                     /locus_tag="QGH35_02855"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HJL81507.1"
                     /translation="MIDVFRIIFLRDLKVSLRRWSDVFISLIFFILVCLIFPLTAGDD
                     QILLLKIIGPVMLISALLATLLSMQSLFKPDVSNGSLEQLVISSNSLAYVVCIKIFCF
                     WLVTVLPLLFVSGFIAASYYVDQQSILIVILTLLVSTPTFVNIAALAAALTVGVRHSG
                     ALMALIIIPLTLPAMIFGSKAIEYSILGFDPTAPLYFLIGMMIFTSTLGPFVTAAAIR
                     ISID"
     gene            complement(5223..5843)
                     /gene="ccmA"
                     /locus_tag="QGH35_02860"
     CDS             complement(5223..5843)
                     /gene="ccmA"
                     /locus_tag="QGH35_02860"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HJL81508.1"
                     /translation="MIKHNLKSECLELFRNDDLIFSDVSFDLSPGQHLIVNGSNGSGK
                     TSLLRTICGLTVPSGGLLYWDGIESQTIDSNFYSYLAYLGHKNGLLAELTGKENLEYT
                     LGISATEKDQYRIIEGFNLTEFMDQLVVKLSHGQQRKIALSKIVQSGKILWILDEPFA
                     NLDGKGTAFLISEIQSHLNQGGMLISTENTDQPIIDSDINFNLDAP"
     gene            complement(5881..>6860)
                     /locus_tag="QGH35_02865"
     CDS             complement(5881..>6860)
                     /locus_tag="QGH35_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017655669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HJL81509.1"
                     /translation="DQGLIFTERGKPSPCLGEVIVKIKAASVNYRDFLIAHGFYNPNL
                     SLPLIPLSDGAGEVVEVGNDVKSFKPGDRVCSMMFQNWHNYSQGWSKSRSISTGCDAA
                     GVLTEYAVLPESALVKMPDNLNFAEASTLPCAAVTAWACLQTADIQKGDTVVLMGTGG
                     VSIFGLQFAKALGANVIITSSSDEKLKRAEVLGADGLINYKENTDWGRQAFELSGGAK
                     LVIEIGGAGTLVQSMEALGVGGHICSIGVIKGGNIEINLFQIMFKNIHLHGITVGPRE
                     IQQQMNEFIAQHDIHPVIDREFDFEDSPGAIMGIKAGNHFGKIVINMDI"
BASE COUNT         2306 a         1435 c         1169 g         1950 t
ORIGIN      
        1 ttattaggtt tcccggagat taggtcaaag aaaaactgca cgagaacaat aatataaaga
       61 agaaacctac atacgccaaa agcaaacaag aacaaaagaa cgtagagtcc ttttatcaat
      121 cgatccttga ctggataggt ttcttccatt aactactcat tcagtaaaac tgacacaaaa
      181 tactattagt tgacttaatt tacaagggtc atatcactgt ctctttatac attattgttg
      241 ctggaataaa tgtgtatgta ttatggagtt ttatatcgtg attaatttat tctctggtat
      301 caatcaatgg cgattttatc ttacgatacc acccaaatat tagcagcaaa agaatcagtg
      361 gagaaaacca caatagtaat gttttcttat taaaagacgg ctcatataaa acaaattccc
      421 cataacgatt caacaaaaat tccttgattt caagttcggt cttaccctca tttatctggt
      481 tgtgtatttg aattcttaag tctttagcca gatctgcatt agattctgcg atcgattgat
      541 tctggcatac cagacaccta acttcttgaa tgatatgttg ataaagagct tcttgctcag
      601 atgtgaattc accctgggcg attgatgatg aaatgcttat gaaaagaata aataataaat
      661 tacagatagc tttgttcatt gttgcatttt gttaatgact ggtaagattt ctcgattcca
      721 gatctgggtt gataaaggcc ctatatgttt gtattttata acgccctcct cgtctatcaa
      781 aaaggtctca ggcgcaccat agacaccaaa atcgatagct gtttttccta ttgaatcaac
      841 caatacttca gaatatgggt ttcctgattc tttaagccat aactgtgcaa gttctgtttc
      901 atctttccaa tttataccca cgattggaat ggcctgtggg gagttgctaa gctgtactaa
      961 aaactcgtgt tcctctaaac aacccggaca ccaagtagcc cagatgttga ttaatgatat
     1021 ttgacctatt atgtcttgtt cagttattaa ttgatctgtc cccagagtag gtagattaaa
     1081 ggcaggcatg ggtttatcga ttaatggtga gggaatattg ctttgccctt ggtacaaacc
     1141 tttaaacaat aggaaaaaaa atgctagaaa aatcaatagt ggcaagtact tcctaatcat
     1201 cctgctgcac caattttctt cttctaagtg gtttggtcaa ataaactcta aacaaaccac
     1261 ctataatcat tacaaagggc ccgatccaga ttaatcttaa cagtggtttg tattgtaaat
     1321 gcaagctcca agtctgatca ccaacattct cgcccaaagc aacaaacaga tcccttgcaa
     1381 tatttgattc aattccggcc tcggtcattg cgggtgaatt tcgatcataa atccttttct
     1441 caggataaat aaccgtgata tcactatctt ttgaggatat ttgaatttca gcaataatag
     1501 aaaagtaatt tggtcctaca ctttccttaa tatcattcag ttgaaagata tagtcgccta
     1561 cttgcgttga ttgaccaaaa cgaagactct tgtcttgggt aagtccatac gaactggtaa
     1621 ctgtaatacc caagacagtt aaacccaaac caatatgagc tagagacata ggtaaaattt
     1681 tcatagcatt ttctaaagta ttcatacttg aaatcatgac gagtcttgtt gaaaacatta
     1741 ttattcgctc gatcaaaggc attaacccac tcagaatcgt ccatccggct aaaatgatac
     1801 cgattaaagt caaaaaacca gcatagccat agaaaaaaac tgtgcatcca ataccgaaaa
     1861 gaatggatgc aaccaaaatc catttaagtt tagagatcat tattttagtg ttggaaaatt
     1921 tccatgaggt gaatgcacct aaaccaataa agaacaacaa aggtaaaaaa ggaattaaaa
     1981 aaacaaaatt aaaataaggt gctccaactg atatctttcc agcatcaaat atttcaagag
     2041 ccaaaggata gagtgttccg atgagtatca agcctgatgc ggtacataga aagacattat
     2101 ttaacaacaa taatgatgct ctggagagta tgtcaatttt cactggtttt tcctttttat
     2161 atttcattgc cataagcaac aaagcaccac ctgtaaataa acccagtagc caaagtataa
     2221 aaatacctct acttggatca gaggcaaaag aatgtactga gatcaatatg ccggaacgaa
     2281 ctaaaaatgt acccaataaa ctctgtgaaa aggaaatgat cgctaacaag atactaaacc
     2341 cattaaacag tttttgccgt tcggtgacga taagtgaatg caataaagcg gttgtggtta
     2401 gccaaggcat taaggatgca ttttcaactg gatcccagaa ccaccatcct ccccatccca
     2461 gttcataata agcccaccaa ctccccaata cgattccccc ggttaaaaat acccaagcca
     2521 taatcgtcca gaccctagca atgcttgccc aatggaattg ggagtggttt ttctttgagt
     2581 tcattaatga agacaatgcg agagcaaaag gaacggctaa acccacgtaa ccggcgtaga
     2641 gcatcggtgg gtgaataacc agtgcaggat cttgtaaaag tggattcaaa cttctccctt
     2701 ggataggtgg attaggtaac acctcaaaag gattagatgt gaaaatgatg aaaagaacaa
     2761 agctaaagct tattaaacct aaaataccca atgcagttat tttaagatct aaatattgag
     2821 aattatgttt ctttgaaaaa gccaataaaa aagaccacat ggagagtaag aacaaccaca
     2881 ataagaaaga gccttcatga gctccccaaa tcgctgcaat cttgtagaga atgggtagat
     2941 ttgtattaga attaagtgct acatacaaaa cagaaaaatc atcagatacg aaactatatc
     3001 caagacacac catggccaag aataatccga cagtatgaat ggtcacgatt ctgctcatta
     3061 atctatcgcg ttgatctgta cttattttag taagacccat tcccagaaat gattgaatga
     3121 atgctgcaac cattgcgatc accagcaata attgtcccag ctcaggtatc atctatgatt
     3181 gttccttaga ataattaggt tgtttctttg attttatgaa tttcaggtgg catatagttt
     3241 tcatcatgct ttgccaatac ttctctggct ataaaaacgc cttcagtatc caatcttcct
     3301 gttgcgatta taccctgccc ttccctaaat aaatcaggca atattccatt gtatttaact
     3361 attatgaaaa cattaccatc cgttaagaaa aactcgacag caactgaacc ttctgctctt
     3421 ctaacactac cgggttgaac caatcccccc attctaaatt ctctatcgat tggtgcttct
     3481 cctgattgaa cttgaatggg agtaaaatag tagagaagat tctgctgaaa ggacttcaat
     3541 acaaaaaaag aagccaaccc aactcccaat agaacaacga tcaaccccag tattctccta
     3601 tgtcttggta gcatttttta gatctatccc tggctttttt taatgaatga ttttacttcc
     3661 aacaacctgg atctaaatcg tttgcgagcc gtcagccaat taagcaagat aacaagaaca
     3721 gagactagaa cactacccca aacataaaat tcatagggtt ccccattaac accattcatg
     3781 gaaaaaatct cagacattgt cattctcttc actgaagatg gaccctatcc accttgtttt
     3841 cttttctcgg ttcattatct cagcagctgt tcttctcatt ataattccaa agaaaaaaag
     3901 tgaaaagccc agaatcataa tcattagagg ccacaacatt tcagatgcaa tggcaggctt
     3961 ggctaatctt gaaagagtag atgcttgatg aagagtattc caccaaacaa ctgaatagtg
     4021 aataatggga atattcacca gtcccacgat tgcaaacaag gaagctgatc gatcggcttt
     4081 acttctatca ggaatagata gatataataa aatataagca agatagagaa ataatagaat
     4141 caattcagac gttaatctcg catcccatac ccaagcagaa ccccacatag gcttacccca
     4201 aagcataccc gttataaggg ctaaaaaagt aaaagatgct ccaactggtg cacaactgtt
     4261 tgcaaatgaa aaagcaacat tggatcgcca gaccagacca accactgaac ccattgccat
     4321 tgcaacgtat gccatcattg acaaatatgc actcggcaca tgaaagtata tgattctgta
     4381 agcatcaccc atttggtaat caggtggtgc tatgaatagc cctccataca ttccaattga
     4441 tatcaaaagc aaagcacatc ctaaaaacca aggctctagt aaaatcgcaa gtttaaagaa
     4501 ataaggtggg gaaccgagtt tgtatatttt tcttagattc atgagttgcc aaccattcta
     4561 atcgattgag attctaattg ccgctgccgt tacaaatggc cccaaagtac tggtaaaaat
     4621 catcattcca atcaaaaaat agagcggtgc tgtaggatcg aaccctaaaa ttgaatactc
     4681 aatagctttg gaaccgaaaa tcatagctgg gagtgttaag ggaataatga tcagtgccat
     4741 tagagcacca gaatgacgaa caccaaccgt taatgcagca gccagtgcag caatattcac
     4801 aaacgttgga gtgctgacta aaagagttag tatgacaatc aatattgact gctggtcaac
     4861 ataataactc gcagcaataa accctgagac aaacaataaa ggcaatacag tcactagcca
     4921 aaaacaaaat atttttatgc aaactacata cgctagtgag ttagaactga taactaattg
     4981 ttcaagtgac ccatttgaaa catctggctt gaaaagactc tgcatggata atagagttgc
     5041 caaaagagcc gatattagca tcactggccc gataatcttc aacaacaata tttgatcatc
     5101 gcctgcggtt aaggggaata ttaaacacac aagaataaaa aaaatcagac tgataaaaac
     5161 atcgctccat cttctcagtg acacttttaa atctcttaaa aaaataattc tgaatacatc
     5221 aatcatgggg catccaaatt aaaattaata tccgaatcaa tgatcggctg gtcggtgttt
     5281 tctgttgaga tcaacatgcc tccttgattc agatgggatt gtatttcaga aattaagaat
     5341 gccgtcccct tgccatcaag atttgcgaat ggctcatcta aaatccaaag tatttttcct
     5401 gattggacaa tttttgatag ggcaattttt ctttgttgtc catgcgagag cttgactact
     5461 agctgatcca tgaattcagt taaattaaat ccttctatga ttctatactg gtctttttca
     5521 gtagcactga tgccaagtgt atactctaga ttctccttcc cagttaattc agccaacaga
     5581 ccgtttttat ggccaagata agcaagatag ctgtagaaat tactatcaat ggtttggctt
     5641 tctataccat cccagtacag caaaccaccg gagggcactg ttaaaccaca aatggttctc
     5701 aacagactgg ttttgccact gccattggag ccatttacaa ttaggtgttg tcctggggac
     5761 agatcaaaag atacatccga aaatatcaag tcgtcatttc gaaataattc aaggcattct
     5821 gatttaagat tatgctttat caaagatcct ctccttgtct aagtcatgct gatttagaaa
     5881 ttatatatcc atattaatga ctatttttcc aaagtgattg ccggctttaa ttcccataat
     5941 cgcaccagga ctgtcctcaa aatcaaattc ccgatcaatg acaggatgaa tgtcgtgctg
     6001 ggcaataaac tcattcattt gttgttgtat ttctctagga cctactgtga tcccgtgtaa
     6061 atgaatattt ttaaacataa tttgaaacag attaatttct atgttccctc ccttgattac
     6121 accaatggag caaatatgac caccaacgcc caaggcttcc atactttgta ctaatgttcc
     6181 ggctccacct atttcaatga ccaacttggc accaccagac aattcgaagg cttgcctacc
     6241 ccagtcagta ttttctttgt aattaatcaa cccatcagca cctaaaacct cagcccgttt
     6301 caatttttca tcactgctgg aagtaataat tacattggca ccaagggcct tagcaaattg
     6361 caatccaaaa attgagacgc cacccgttcc cataagtaca acggtgtcac ctttttggat
     6421 atcagctgtc tgtaaacaag cccaagctgt cactgcggca caaggaagag tggatgcttc
     6481 tgcaaaattc aaattatcag gcattttaac taaagcagat tcaggtagaa ctgcatactc
     6541 ggttagcact cctgctgcat cacaaccggt agatatggat cttgatttcg accaaccctg
     6601 gctatagtta tgccaatttt gaaacatcat cgagcatact ctgtcacccg gcttaaaact
     6661 tttcacatca ttaccaactt ctacaacttc accagctcca tccgacagag gaattaacgg
     6721 tagtgaaaga tttggattgt aaaaaccgtg agcgatcaaa aaatccctgt aattaaccga
     6781 agctgcttta atctttacaa tcacttcacc cagacagggg ctaggttttc ctctctccgt
     6841 aaaaattaaa ccttgatcgc
//
LOCUS       DASCTH010000135         3013 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000182778, whole genome shotgun sequence.
ACCESSION   DASCTH010000135 DASCTH010000000
VERSION     DASCTH010000135.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000182778"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="QGH35_02870"
     CDS             complement(<1..365)
                     /locus_tag="QGH35_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009024781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HJL81510.1"
                     /translation="MHSPNKVHLVIVNSAKGSIESDNEEFISLVLSAGAVINEITYFY
                     ARRFFRRSLIGKGKIREIAQQIIIKDSDLVIFNNSLTASQERNLELDLNCRVIDRTRL
                     ILDIFAQRAVTNAGKLQVE"
     gene            complement(392..1321)
                     /gene="miaA"
                     /locus_tag="QGH35_02875"
     CDS             complement(392..1321)
                     /gene="miaA"
                     /locus_tag="QGH35_02875"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HJL81511.1"
                     /translation="MNNSKIIILKGPTASGKTDLAITLYDKHPLEIISVDSVMVYKGL
                     NIGSAKPGPKDLKLYPHHLIDIREPTASYSVGEFHKDVKGLIESIIEKGKTPILVGGT
                     MMYFRVLQQGLSTLPSANQEMRHQIDLEATEKGWLNLHKELQMIDPKSAQRIKPSDKQ
                     RIQRAIEVYRITGKTISSFYQEQKQPLNYQCLDISLFPNDRASLHKRIEERFNAMLKK
                     GLISEVENLLSIDLLTSSHQSMKSVGYKQICEFIENKCSLEEAKSNAIFASRRLAKKQ
                     LTWLRSKQKSFFVDCFDPQFANIIEDKVSQFLV"
     gene            complement(1340..2365)
                     /locus_tag="QGH35_02880"
     CDS             complement(1340..2365)
                     /locus_tag="QGH35_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJL81512.1"
                     /translation="MDIDIILEPNLPPSQVKELGIIAEEYGIRALWTSNYFSHWDGFL
                     SLVPLAESTKKLVFGPLAVSPFEMHPLKIVNALLTLNEMSNGRTQIAICAGEGNVTAM
                     DLLPPKKIVLAVRESIEILLAACHNQLQEPYEGEIFRVKLPCDYSWAKSTRPTIYGTA
                     YKHMMMRMEGRVADGVYIGAIPPEIIDSAIENIKKGINRREKPNQHIHINSFWGWHIK
                     KDKKNAYRESRRELAWRARKLDPELLSLFFNPDECKLIVNNFQCFVDAYFDRSGNVKN
                     IPEELSNKLCEVFTSTGGLEDIDREIERIKKFEKAGLTQLSLRLHDDPMEALQIIGEN
                     VVPVFSQ"
     gene            complement(2380..>3013)
                     /locus_tag="QGH35_02885"
     CDS             complement(2380..>3013)
                     /locus_tag="QGH35_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HJL81513.1"
                     /translation="KRLAHLINAEPDMTAILTRNDDSFPCPNNNRSCGQKTSLNERLE
                     IAEKAGAVLFISIHANSFRDQRIRGAEIYALPDSAKFEKDENWKVMYHKKPSKSRIKL
                     IDVTSGKQIKQVTANEVIAFDQSLQISDNIIQELRKVIRVRKSTRQAAFIVLGSNKMA
                     SVLIETSYLTNPEDEKLLISPVNQQKIAEAILKGVKKYTREGRQQFTKQK"
BASE COUNT          847 a          586 c          501 g         1079 t
ORIGIN      
        1 aattcaacct ggagtttgcc agcattggtt accgctcttt gtgcaaagat atcaagaatc
       61 aatcttgttc tgtcaattac tctgcaatta agatctaatt ccagattcct ttcttgagat
      121 gcagttaagg agttattaaa gatgactaaa tcactatctt ttataattat ctgttgtgca
      181 atttctctaa tcttaccctt gccaattaga cttcgacgaa aaaagcgacg agcataaaaa
      241 taggtaattt cattaatgac tgctcccgct gaaagaacga gtgaaatgaa ttcctcatta
      301 tcagattcaa ttgagccttt tgctgaatta acaattacta agtgaacttt gtttggcgaa
      361 tgcatttgtc gtaaacataa ttttataaaa gttagactaa gaattgagac actttgtcct
      421 ctataatatt tgcaaactgc ggatcgaaac agtctacaaa aaaactcttt tgtttagatc
      481 taagccatgt aagttgtttt tttgccaacc ttcttgaagc aaatattgca tttgatttag
      541 cttcctcaag cgaacattta ttttcaatga actcacagat ttgtttatag ccaacagact
      601 tcatcgactg atgagatgag gtaagcaaat ctatggataa taagttctcc acctcagaga
      661 tgagcccttt ttttaacatg gcattaaatc gttcctcaat gcgtttgtgc aagcttgctc
      721 tatcattggg aaataaggaa atatcgaggc attgataatt cagtggttgt ttttgttcct
      781 gataaaaact ggatattgtt tttcctgtga tcctatagac ttctattgct cgttgaatcc
      841 tttgtttgtc tgatggtttt attctctgag cagacttagg gtcaatcatt tgcagctctt
      901 tatgtaaatt taaccaacct ttctcggtcg cttctagatc gatttgatgt cgcatttctt
      961 gattggcact cggtaatgtt gacaatcctt gctgcaggac tctgaaatac atcatggttc
     1021 ctcctactaa gatcggtgtt tttcccttct caataatgga ctcgatcaaa cccttaacat
     1081 ctttgtggaa ttcaccaact gaataacttg cagtgggctc acgtatatca atgagatggt
     1141 gagggtataa ttttagatct ttagggcctg gtttggctga cccaatattc aatcccttgt
     1201 ataccataac ggaatcaaca ctgataattt ccaaaggatg cttatcatac aatgtgattg
     1261 caagatcagt ttttccactt gcagttgggc cttttagaat aataatttta ctattattca
     1321 aaataaagaa agcttctatt cattgactaa atacaggcac cacattttcc ccaataattt
     1381 gtaatgcttc cataggatca tcgtgcaaac ggagtgagag ttgagtcaat cctgcttttt
     1441 caaatttctt aattctttct atttccctat cgatatcctc aagaccgccc gttgaagtaa
     1501 acacttcaca aagcttattt gataattcct ctggaatatt ttttacgttt ccagaacggt
     1561 caaaataagc atctacaaaa cattgaaaat tattgactat taatttgcat tcatcagggt
     1621 tgaaaaaaag gctgagcaat tcaggatcaa gttttcttgc acgccaagcc aactctcttc
     1681 tagattcacg ataagcattt ttcttatctt ttttgatatg ccatccccaa aaactattga
     1741 tgtgtatgtg ttgattaggt ttttcccttc tattgattcc tttttttata ttctcaatgg
     1801 ctgaatcaat aatttcaggt ggtattgctc cgatgtatac tccatcggca actcgccctt
     1861 ccattctcat catcatgtgt ttgtaggctg tgccatatat ggttggcctt gttgatttcg
     1921 cccaactgta atcacaagga agtttaaccc taaatatctc accctcataa ggttcttgta
     1981 attgattatg gcaagctgca agaaggattt ctatcgattc tctaacagct aaaactattt
     2041 tttttggtgg caataagtcc atcgcagtaa catttccttc tcctgcacag atcgcaattt
     2101 gtgttcggcc attgctcatt tcgtttaagg ttagtaatgc attgacaatt tttaatgggt
     2161 gcatttcaaa tggactgact gctagaggac caaatactaa tttttttgtt gactctgcca
     2221 aaggcactag gcttaaaaac ccatcccagt gagaaaaata gttagaagtc cataatgctc
     2281 gaatgccata ctcttctgca atgattccta gctccttaac ttgtgatgga ggtaagttgg
     2341 gttctaatat aatatcaata tccatagtta ctctttactc tatttctgtt tggtaaattg
     2401 ttgtctgcct tcacgggtat atttcttaac tccttttagg atagcttctg caattttttg
     2461 ttgattgact gggctgatta ataatttttc atcctctgga ttagttaaat aacttgtttc
     2521 tatcaacact gaagccattt tatttgaacc caaaactatg aatgctgcct gccttgtaga
     2581 ttttctcact ctaattactt ttctaagttc ctgtattata ttatcactga tctgcaagct
     2641 ctgatcgaat gcaatcactt cattggcagt cacctgttta atttgttttc cagaagtcac
     2701 atcgattaat ttgattctag attttgacgg ctttttatgg tacatcactt tccaattttc
     2761 atctttttca aacttggcag agtctggcaa tgcataaatc tcagctccac gaattctttg
     2821 atctctaaaa gaatttgcat gaattgaaat aaacagtaca gcacccgcct tttctgctat
     2881 ctctaaacgc tcatttaatg aggttttctg tccacaagat ctattgttat tcgggcatgg
     2941 aaaagaatca tcgtttctag tcaaaatagc agtcatgtct ggttcagcat tgattaagtg
     3001 tgctaaacgt ttt
//
LOCUS       DASCTH010000136         6940 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000185513, whole genome shotgun sequence.
ACCESSION   DASCTH010000136 DASCTH010000000
VERSION     DASCTH010000136.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000185513"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="QGH35_02890"
     CDS             <1..583
                     /locus_tag="QGH35_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024498.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HJL81514.1"
                     /translation="LMKEILIKTVITCILLFSNVLLSQDGIDIKDSEASQTNEFEEYK
                     KQVIASLFGALDEQQMVIFSPERFQHTVTVFTDIDCGFCRKFHNEIDQVMEQGIRIRY
                     LFFPRTGPFSESWEKAEQVWCSQDQHSAMTKAKKGQNLTVRVCPNTPVEKHFNIGQLL
                     QVQGTPTIISDKGEIIVGYLPAEALKARLESNL"
     gene            complement(585..908)
                     /gene="fdxA"
                     /locus_tag="QGH35_02895"
     CDS             complement(585..908)
                     /gene="fdxA"
                     /locus_tag="QGH35_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HJL81515.1"
                     /translation="MTWVVGEQCINCKLGDCVEVCPVDCFHEGPNMLVIDPDECIDCA
                     LCEPECPVDAIFADDEIPEGQEKFLELNKELSQIWPVISEAPIPPDDADDWRDVKDKL
                     KYLEK"
     gene            complement(977..1504)
                     /locus_tag="QGH35_02900"
     CDS             complement(977..1504)
                     /locus_tag="QGH35_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HJL81516.1"
                     /translation="MMKYCSQCGGETKLEIPQDDDRPRFICSQCGFIHYQNPRLVVGS
                     IPVHEDQVLLCLRAIEPRKNYWTLPAGFLENGESISDGAIRETEEEALITPNLGEIIA
                     IVDVVYAEQVHVFFRAHLDNLDFGPGRESLDVKLFKLTEIPWEEIAFKTVKIALKAQI
                     NKASNDSIPIYETIR"
     gene            complement(1522..1605)
                     /locus_tag="QGH35_02905"
     tRNA            complement(1522..1605)
                     /locus_tag="QGH35_02905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1569..1571),aa:Leu,seq:caa)
     gene            1713..2744
                     /gene="queA"
                     /locus_tag="QGH35_02910"
     CDS             1713..2744
                     /gene="queA"
                     /locus_tag="QGH35_02910"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HJL81517.1"
                     /translation="MKLTDYNFDLPEELIAQQPNIKRISSRLLHVKGYVVKDRVFSEL
                     PELLKKNDLLIVNNTKVMSSRLFGKKITGGKVEILIERILDTTMALIQTKSSKRPLLG
                     DIILLDNEVSIEIIGIEQDFLRGKFSEDINCVLDEIGHVPLPPYISRLPNINDKKRYQ
                     TVYATKSGAVAAPTAGLHFSKSLLNAIQNKGIRIGTITLHVGSGTFMPLRAEQLKTKK
                     LHREQTVVGEDLCREIEHTLSIGGRIIAVGTTVTRALETAALSGRIKPFAGETDIFIY
                     PGFQFRIVDAMITNFHLPKSSLIILVSAFIGREEVLKAYQHAVNQHYQFYSYGDAMFL
                     ERSNSKRCK"
     gene            2741..3850
                     /gene="tgt"
                     /locus_tag="QGH35_02915"
     CDS             2741..3850
                     /gene="tgt"
                     /locus_tag="QGH35_02915"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HJL81518.1"
                     /translation="MNFEILYQDSMARCGQLSFLRGEIHTPVFMPVGTYGTVKAMTPD
                     ELLSLGAEIILGNTYHLMIRPGEEVIALHNGLHKFMGWEKPILSDSGGFQIWSLSKKT
                     KVHDDFVEFDSPINGDIIRLTPEKAMKVQSILGSDIQMILDECTHYPANKIDAQSSML
                     RSAQWAKTSFDAFEKYKKNDDTAIFGIIQGGMYPDLREDSLDKMVEIDFDGLAIGGLS
                     VGETEQERRDVLASLVPKMPKHLPRYLMGLGTPIDIVKAVSVGIDMFDCVIPTRHARN
                     GQIYTSQGLLRIKNAQYQKDTRPLDANCSCYTCQNYSRSYIRHLANCNEILGSRLATT
                     HNLFFYLDLMRQIRDSIQKNEFKKFAEAFIQGYQQ"
     gene            3898..4224
                     /gene="yajC"
                     /locus_tag="QGH35_02920"
     CDS             3898..4224
                     /gene="yajC"
                     /locus_tag="QGH35_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HJL81519.1"
                     /translation="MNIFIENAWAQSGESGGDPLLGFLPLIIIFVVFYFLLIRPQSKK
                     TKEHKEMIASLVVGNEVITAGGILGKIIEINEQYVHLEISKGVTVKVQRHTVGTLMPK
                     GTIKKG"
     gene            4242..6077
                     /gene="secD"
                     /locus_tag="QGH35_02925"
     CDS             4242..6077
                     /gene="secD"
                     /locus_tag="QGH35_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007041237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HJL81520.1"
                     /translation="MNQYPLWKNLMVLAVLILGIWFALPNFYGEDPAIVISKENGQVF
                     SASEVGEIEEYLQEQSSRYTSINQKSNQILIRFDQVEEQLFASETMRPYLGRTATVAL
                     SFAPRTPNWINAVGLQPMSLGLDLRGGVHFKFQVDLGAAIEQRLNQYIADINKALIDK
                     RIRRSVTKQGNVINIEFSENNDLSVARTLIREIDPLLQITKVTDTILQVQMTEDQLKE
                     RRDFAIQQNILTMRNRVNELGVAEPIVQRQGLDRIVVQLPGVQDPTQAERILGATATL
                     EFRMVCENESAFDAEAKGRAPIGCELFADRGGNPVLLKKKTIVSGNQLIDASQTFSEG
                     RPAVSIRLDSKGSREMLETTKRNLNKPMAVLFIQQRQETKRQNGELITSSVLDKEVIN
                     IATIRGVFSNQFQITGVSVFEAKDLAILLRAGALAAPIFKVEEKTVGPSLGQDNINRG
                     FQAMLIGLALVLVFMIIYYKGFGLIANIGLLINLTLVMALLSLLQASLTLPGIAGIVL
                     TVGMAVDANVLIFERIREEIRIGNSPQASITAGYQKAFGTIADANITTLIAALVLFTF
                     GTGPIKGFAVTLTLGIITSMFTAIIASRAIVNLVYGQRTISRLRI"
     gene            6084..>6940
                     /gene="secF"
                     /locus_tag="QGH35_02930"
     CDS             6084..>6940
                     /gene="secF"
                     /locus_tag="QGH35_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HJL81521.1"
                     /translation="MHKKEYNFLSIRYVAMAFSILLLLISLGSLMTKGLNKGIDFTGG
                     FLIEVSYQQPPTISQVRSELAVGGFPDAIVQNFGGSKDLMIRILPREGADNKVVGKQI
                     IEILDNSSNKAELRGSEFIDPQVGEELTNDGALAMIFALIMIMIYIIIRFQWKFSIGA
                     VAALIHDVLITIGVFSFFQISFDLAVLAAILAVVGYSLNDTIVIFDRIRENFRLIRKM
                     ETLSILNKSVNQTISRTIITSSTTLLVLLALYLIGGKSLEGFSLALIIGVIVGTYSSI
                     YVATTSIFL"
BASE COUNT         2185 a         1248 c         1398 g         2109 t
ORIGIN      
        1 gcttatgaaa gagattctta taaaaacagt tataacttgt attctgttat tcagtaacgt
       61 cctattgtcc caagatggaa ttgacatcaa agattctgaa gcttctcaaa ctaatgaatt
      121 tgaagagtat aaaaaacaag ttatcgcctc attatttggt gcgcttgatg agcaacaaat
      181 ggtcattttt tcaccagaaa gatttcaaca cactgttaca gtttttaccg atattgattg
      241 tggattttgt aggaaattcc ataatgaaat tgatcaagtt atggaacagg gtattcgtat
      301 tcgttatctt ttttttccaa gaactggtcc ctttagcgag agttgggaga aagcagaaca
      361 agtttggtgt agccaagatc aacacagtgc tatgactaaa gcaaaaaaag gtcaaaatct
      421 aacagttaga gtttgcccta atactccggt agaaaaacac tttaatatcg gccaactatt
      481 acaagttcag ggtaccccaa caattatctc tgataaaggc gagatcattg taggttattt
      541 accagcagag gctttgaagg caagactaga atccaacctt taatttattt ctcaagatat
      601 ttgagtttat ctttcacatc ccgccaatcg tctgcatcat caggtggaat tggggcttca
      661 gaaatgaccg gccagatttg agataattct ttgtttaatt ccaagaattt ttcttgcccc
      721 tctgggattt catcatcggc aaaaattgca tcaacaggac attctggttc acacagggca
      781 cagtctatgc attcatccgg gtctataacc aacatattgg gaccttcatg aaaacagtca
      841 acaggacaaa cttctacgca atcacctaat ttacaattaa tgcattgttc accaactacc
      901 caagtcataa taatctccag atttaaaaaa ttttaaaaat catgccacaa acattgaatc
      961 atttaaagtg gattactcat ctgatagttt catagatggg aatactatca ttgctcgctt
     1021 tattgatctg tgctttgagt gcaattttta ctgtcttaaa tgcaatttct tcccaaggta
     1081 tttcagtcag cttaaacaat tttacatcca aagattctct acctggacca aaatctaaat
     1141 tatcaagatg ggctctgaag aacacatgaa cttgctctgc ataaacaacg tctacaattg
     1201 caatgatctc tccaagattt ggcgtgatta acgcttcttc ttctgtttct cttatagcac
     1261 catctgaaat actttctcca ttttcaagaa atcccgcagg cagggtccag taatttttcc
     1321 ttggttctat ggcccgaaga cataaaagta cttggtcttc atgaaccgga atagagccaa
     1381 caactagcct aggattttgg tagtgaataa agccacattg gctgcaaata aatcttggcc
     1441 tatcatcatc ttggggtatt tcaagcttag tttcgccacc acattgtgaa caatatttca
     1501 tcatatattt ctaccaataa ctgccgggag agagactcga actctcacgg gtttaacccc
     1561 accggatttt gagtccggcg cgtctaccaa ttccgccatc ccggcttaat attagaaaga
     1621 gaataccttg gcaaaaattt acagtccaca gtctttctaa attttttaat cgatattatt
     1681 ttctaatcga ataattcagt agaatactca aaatgaaact cacagattat aattttgatc
     1741 ttcctgaaga actgattgct cagcaaccaa atataaaaag aatttctagc aggttgcttc
     1801 atgttaaagg ttatgtagtc aaggatagag ttttttctga gctcccggaa ttactcaaaa
     1861 aaaatgactt gttgattgta aataacacca aagtcatgtc ttctcgctta tttggaaaaa
     1921 aaataaccgg tggcaaggta gaaatcttaa ttgaaagaat tttagatact actatggcct
     1981 tgattcaaac aaagtccagt aaacgtccac tgctcggaga tatcatcctt ttagacaacg
     2041 aagtttcaat agaaattata gggattgaac aagattttct taggggaaaa ttttcagaag
     2101 atatcaattg tgttcttgat gaaatcggac atgttccgct gccaccttat atcagtaggc
     2161 tacccaatat aaatgataaa aagagatacc aaacggtcta tgcaacaaaa tcaggagctg
     2221 tagccgcacc cactgctgga ctgcacttta gtaaaagtct gctcaatgca atccagaaca
     2281 aaggaattcg tattggtaca attacccttc acgttggcag tggcacgttt atgccattac
     2341 gtgcagaaca acttaaaact aagaagttgc atagagaaca aactgtcgta ggggaagatc
     2401 tttgtcggga aatagaacac acattatcta ttggtggaag aattattgct gtgggcacaa
     2461 cggtgactcg tgctctggag acagcggctt tatccggcag gatcaaacct tttgctggag
     2521 agacagacat cttcatctat ccaggatttc aatttagaat tgtggatgca atgattacta
     2581 attttcattt acctaaatcg tctttgatca tactagtttc cgcatttatc ggaagagaag
     2641 aagtattaaa agcatatcaa catgccgtca atcagcacta tcaattttat agctatggtg
     2701 atgcaatgtt tctagaaaga tccaattcta agaggtgtaa atgaatttcg agattttata
     2761 tcaagattca atggctcgat gtggtcagtt gtctttctta agaggggaaa ttcacacccc
     2821 tgttttcatg ccagttggta catatggcac tgtcaaggca atgacaccag acgaattatt
     2881 gagtttaggt gcagagatta ttttaggaaa cacctatcat ttaatgatta gacctggaga
     2941 ggaagtgatt gcattgcata acggtttaca taaattcatg ggatgggaga aacccatatt
     3001 gtctgattct ggcggctttc aaatctggag tctttcaaaa aaaaccaaag tccatgacga
     3061 ttttgtagaa tttgattctc ccatcaatgg ggatatcatt cgattaacgc ctgaaaaagc
     3121 aatgaaagtt caaagtattc ttggcagtga tattcaaatg attcttgatg aatgtactca
     3181 ttatccagca aataaaatcg atgctcagtc ttcaatgttg cgttctgcac aatgggcgaa
     3241 aaccagtttt gatgcgtttg aaaagtataa aaaaaatgat gacactgcaa tttttggaat
     3301 cattcagggg ggcatgtatc ctgacttaag ggaagatagt ttggataaaa tggttgaaat
     3361 tgactttgat ggtttggcca tcgggggatt atccgttggg gaaacagaac aggaaagaag
     3421 agatgttttg gcatcattgg ttccaaaaat gcctaaacat cttcctagat atttgatggg
     3481 tcttggcact cctattgata tcgtcaaagc cgtatctgta ggcatagata tgtttgattg
     3541 tgttattccc acacggcatg cacgtaacgg tcaaatttat acttcacagg gtcttttgag
     3601 aattaagaat gctcaatatc agaaagatac tcgtcccctg gatgctaatt gctcttgtta
     3661 tacatgtcag aattactcgc gttcatacat tcgacatctg gcaaattgca atgaaatact
     3721 gggttcgcgg cttgcaacaa cacataattt gtttttttat ctcgacttaa tgaggcaaat
     3781 acgagattct atccaaaaaa atgagtttaa aaaatttgca gaagctttca ttcaaggtta
     3841 tcagcagtaa ttgtttatgt gtcataattg cacaattatt ttttacgaag gagcagtatg
     3901 aatattttta ttgaaaacgc ttgggcccag tctggtgaat ctggaggaga tccgttattg
     3961 ggatttttgc ccttgattat tattttcgtg gtgttctatt tcttactgat cagaccacag
     4021 tcaaaaaaga cgaaagaaca caaagagatg attgcaagct tggttgttgg caacgaagta
     4081 ataaccgcag gaggcatact tggaaaaatt attgaaatca atgagcaata tgttcatttg
     4141 gaaatttcta agggggtgac agtaaaagta caacgtcaca cggttggaac tttgatgccc
     4201 aaaggcacca tcaaaaaagg ataaaaaagg atacgagttc tatgaaccaa tacccattat
     4261 ggaaaaatct aatggtctta gcggtcctga tcctagggat ttggtttgca ctgccaaatt
     4321 tttatgggga agacccagcg atcgtaattt ccaaagaaaa cggacaggtt ttcagtgcat
     4381 cagaagtagg ggagatcgaa gaatatttgc aagaacaaag tagtcgttat acatcaatca
     4441 atcaaaaatc taaccagatc ctgattcgct ttgatcaggt tgaggaacaa ttgtttgcga
     4501 gtgaaaccat gaggccctac ttaggcagaa ctgcaacagt tgccctctca tttgcgcctc
     4561 gaacaccgaa ttggatcaat gctgtcgggc tccaacccat gagtttaggt cttgatctta
     4621 gaggaggcgt tcatttcaaa ttccaagttg atttgggtgc agcgattgag caacgtttga
     4681 atcaatatat cgcagacatt aacaaagctt taattgataa gagaataaga aggagcgtga
     4741 ctaaacaagg caatgtcatc aatattgaat tttcagaaaa taatgattta tcagttgcaa
     4801 gaacattgat cagggaaatt gaccccttac tacaaattac gaaagtaaca gataccattc
     4861 ttcaagttca aatgactgag gatcaattga aggaaaggcg tgattttgct atccagcaga
     4921 atattttaac catgagaaac agagtgaatg agttgggagt cgcagaaccc attgtgcaaa
     4981 gacaaggcct tgaccgaatt gttgttcaac tgcctggagt tcaagatcca actcaagcag
     5041 aaagaattct tggagcaaca gcaacacttg aatttagaat ggtttgtgag aatgaaagtg
     5101 cctttgatgc agaagcgaaa ggcagagcgc caattggttg tgaattgttt gcagaccgag
     5161 ggggtaatcc agttttactg aaaaagaaaa caatagtatc tgggaatcaa ttaattgatg
     5221 cttcgcagac cttttcagaa ggaaggcccg ctgtttccat cagactcgat tctaaaggat
     5281 caagagaaat gctggagacc acaaaaagaa acttgaacaa gcccatggca gttttattca
     5341 ttcaacaaag acaagaaacc aaacgacaaa atggggaact gattacttcg tcagtgctgg
     5401 ataaggaagt tattaatatc gcaaccatac gtggcgtgtt tagtaatcaa tttcaaatta
     5461 caggtgtttc tgtttttgaa gcaaaagatt tggccatatt acttcgagca ggggctttag
     5521 cagctcctat ttttaaggtt gaagaaaaaa cggtagggcc cagtctgggt caagataata
     5581 tcaatagggg attccaggcc atgttgatag gtttggctct agttcttgtt tttatgatca
     5641 tttattacaa aggttttggt ctgattgcca atatcggact gttaatcaat cttaccttag
     5701 tcatggcctt attatctctg ttacaggcat ctttaacact gccaggtata gcgggcattg
     5761 tcttaaccgt gggtatggcg gtagatgcta atgttttgat tttcgagaga atcagggaag
     5821 aaatacgcat tggtaattct ccgcaagcga gtatcacagc aggatatcaa aaagcatttg
     5881 gcactattgc ggatgctaat atcacaacct tgattgcagc acttgttttg tttacttttg
     5941 ggacgggacc cattaaggga tttgcggtta cactgacatt ggggattatc acttcaatgt
     6001 ttacggcaat catagcttca agagcaattg taaatttggt ctatgggcag agaacaatta
     6061 gccgattaag gatctagatg agtatgcata agaaagaata taattttctc tcaatacgct
     6121 atgtagcaat ggcattttcc attctgttgt tgctgatttc tttgggttcc ttaatgacaa
     6181 aaggtttaaa taaaggcatt gatttcacag gaggtttttt gatcgaggtg tcctatcaac
     6241 aaccgccaac aattagccaa gtgagaagtg agcttgccgt tggaggattt ccagacgcca
     6301 tagttcagaa ttttggcggt tccaaagatt tgatgattag gattctaccc agagaaggtg
     6361 ctgataataa agttgtcggc aaacagataa tagaaatatt ggataattca tcgaataagg
     6421 ctgaattaag aggatcagaa tttattgatc ctcaagttgg tgaagaatta actaatgatg
     6481 gtgcgttagc catgattttt gctctcatca tgatcatgat ctacattatt attaggtttc
     6541 agtggaagtt ttccatcggc gctgtcgctg ctttaattca tgatgttctt attaccatag
     6601 gagtgttctc attctttcaa atctcttttg atctggctgt tttagcagcc attttagctg
     6661 tggtaggcta ttcattgaat gacacaatcg tgatttttga tcgaattcga gaaaattttc
     6721 gtttaataag aaaaatggaa acactttcaa tccttaacaa atcggttaac caaaccatca
     6781 gtcgaacaat catcaccagt agcaccacat tgttggtgtt acttgcttta tatcttatag
     6841 gtggcaagtc tttagaagga ttttctctgg ctttgatcat tggtgttatt gttggaactt
     6901 actcgtccat ctacgttgcc acaacctcta tatttttatt
//
LOCUS       DASCTH010000137         6023 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000186826, whole genome shotgun sequence.
ACCESSION   DASCTH010000137 DASCTH010000000
VERSION     DASCTH010000137.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000186826"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="QGH35_02935"
     CDS             complement(<1..953)
                     /locus_tag="QGH35_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015155.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HJL81522.1"
                     /translation="MQQIVNNCLKAIVKYPKTVLMILALIGGFFSTWLDDFQLDASSD
                     SLLLEDDLDLRYYRNIKARYGDDEFLVVTYQPQGDLFSQESIDNLEYLRDELNALDSI
                     ESIVSMLDVPLLKSPPQNLSDLEEDVPNLLSASTDRDLAKKELLTSKLFQNLLISSDG
                     NTTAMQLKMVIDENLESLIDARDILREKRLVEPLDENEENKLAILSSQIREQRAVYRL
                     NNVENIADIRAVLDRYRGDNKIFLGGTPMITVDMIDYIANDIEIFGISLLAFLIIALL
                     YIFKRPRWMMISMACCFLGLIVMTGFIGFVGWPVTVVSANFV"
     gene            1087..1653
                     /gene="dcd"
                     /locus_tag="QGH35_02940"
     CDS             1087..1653
                     /gene="dcd"
                     /locus_tag="QGH35_02940"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HJL81523.1"
                     /translation="MAIKSDQWIKKMAEGHSMIEPFVEHQVRHNNGNDLISYGTSSYG
                     YDVRCANEFKIFTNINSAIVDPKSFDDTSFVDLNSDICVIPPNSFALARTIEFFRIPR
                     NILTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPAKIYANEGVAQMIFL
                     ESDEVCQESYKDRKGKYQGQLGVTLAKT"
     gene            complement(1654..2313)
                     /gene="purN"
                     /locus_tag="QGH35_02945"
     CDS             complement(1654..2313)
                     /gene="purN"
                     /locus_tag="QGH35_02945"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011467376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HJL81524.1"
                     /translation="MKKAGMDSRISTVVLISGSGTNLQAIIDEVDQHQLPIKITLVVS
                     DNPNAYGLKRAEKANIKTQIIDYGSYPDKFVFNKIVVQRIKESNPELIVLAGYMRILS
                     DEFCHQFKGKILNIHPSLLPKYQGLNTYDRVLESTDEYHGSTVHFVIPELDSGPIVLQ
                     YRTKIKPHDNPGSLAKRVQQGEYIIYPMAIKMFAEGVLRMIDNQAILNGKPITKARTI
                     E"
     gene            complement(2310..3341)
                     /gene="purM"
                     /locus_tag="QGH35_02950"
     CDS             complement(2310..3341)
                     /gene="purM"
                     /locus_tag="QGH35_02950"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HJL81525.1"
                     /translation="MIKDKSLTYADSGVDIETGNEVVSVIKPLAQDTYSDSVLSNIGG
                     FGGLFELHVKDYKRPILVSSTDGVGTKLKLAQILGKEHLIGIDLVAMCANDVIVQGAK
                     PLFFLDYYATSKLEVAMASRVIQGIAQGCKESNMALIGGETAEMPGIYQSGDFDLAGF
                     CVGVVDFENIIDGSSVEVGNMVIGMSSSGPHSNGYSLIRKIIDIQQSNLESRLKDATL
                     GDALIEPTTIYVKPILNIIKKFPINGMAHITGGGFKENIERILPNHCNAFIDTSSWTQ
                     PDVFNWIQNEGKISEDEMFKTFNCGIGMVVIIKKNYASEIMETFLKLGYQSTQIGYIE
                     NGTGQVMVK"
     gene            3464..4024
                     /locus_tag="QGH35_02955"
     CDS             3464..4024
                     /locus_tag="QGH35_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013250.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HJL81526.1"
                     /translation="MNTSNIPNLICLVRIALVVPIVQFMVQTEFLLSLLLIAIAGLSD
                     ILDGYLAKRNNWRTKLGTLLDPVADKILLVSLFITLFRMELIPFWLTLIVILRDVMIV
                     FGLGLYRYFVDIPEMNPTRISKFNTFTQLAFVLFVIAYQIIPFPFEYLSMLFGALVSV
                     TSIVSGLDYWISWSILAKKKIYQNGD"
     gene            4025..4705
                     /gene="hda"
                     /locus_tag="QGH35_02960"
     CDS             4025..4705
                     /gene="hda"
                     /locus_tag="QGH35_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HJL81527.1"
                     /translation="MEQLLLPVSISEHMVFDSFYPGPNIEIYQSIKIDSQKLLWIAGA
                     KGSGKTHLLQAALHQCFSVSKKGLYIPMRDFKEFNPEILDNLDQLDLICIDDIECVLG
                     KRIWEEKLMGLYEKLQTTKAQLVVGSFESPKGTHFYLPDLASRFAMFMVHKLELLAES
                     ETISAIQLHANSRGFDLPDDCAKYLIRRVQRDVSSLVEIIQLLDYESLSKKRILTIPF
                     IKSILNLG"
     gene            4763..5107
                     /locus_tag="QGH35_02965"
     CDS             4763..5107
                     /locus_tag="QGH35_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81528.1"
                     /translation="MFKSFITMNHSISGPIKILSVALVSTLLLSCATLPPLQEMSNAR
                     QTIAAAKEFSPNAATDKKILEAERLLERAERRIEVTLYEQARLDALRARNEAIEYLEQ
                     AIAQRAISSSPD"
     gene            complement(5121..>6023)
                     /locus_tag="QGH35_02970"
     CDS             complement(5121..>6023)
                     /locus_tag="QGH35_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012796.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HJL81529.1"
                     /translation="DIVRNELKTYRQLPINYYQIQTKFRDEIRPRFGVMRAREFLMKD
                     AYSFHMDQKSLDQEYENMGRTYDTIFTRIGLDYRRVRAGSGEIGGSLSHEYVVLADSG
                     EDKIGYCDEEEFAANVEMIDGDTAPNGGQLSYARGIEVGHIFQLGDKYSKHMNCVVSN
                     EKGNSTHPLMGCYGIGVSRIVAAAIEQNNDQSGIIWPDSITPFQISIISLNQNLEDEV
                     SKKAYEIYQQLLEYGLEVILDDRDERAGVKFADADLIGIPKQLVLSKKGLKSQGIEFK
                     ARGEKSNTSIPFAKLPAFLEKFSS"
BASE COUNT         1834 a         1136 c         1037 g         2016 t
ORIGIN      
        1 acaaaattag ccgatacaac agttaccggc caacccacaa atccaataaa tccagtcata
       61 acaatgagtc ctaaaaagca acaggccatt gagatcatca tccatcttgg gcgtttaaaa
      121 atatagagta gggcaataat taaaaatgct agtagactga tgccaaatat ctcaatatcg
      181 tttgctatgt agtcaatcat gtcgactgtg atcatgggag ttccaccaag aaagatctta
      241 ttgtcacccc tgtaacgatc tagaacggct ctaatgtcag caatattttc aacattgttt
      301 agtctgtaga cagcacgttg ttctctgatt tgactagata aaatagctaa cttattttct
      361 tcattttcat ccaaaggttc tacaagacgt ttttctcgaa gtatatcgcg agcatcaatg
      421 agtgattcta ggttttcatc tatgaccatt ttcaattgca tagctgtagt atttccatcc
      481 gaactaatca gtagattttg aaataatttg ctagtcaaaa gctccttttt ggctaaatca
      541 cgatctgtgc ttgcactcaa cagattagga acatcctcct ccagatctga tagattttgg
      601 ggaggggatt ttaaaagcgg aacgtcgagc atacttacta tagattcgat tgagtctaga
      661 gcattcagtt catctctgag atactctaag ttatcaatgc tttcttgaga aaaaagatcc
      721 ccttgaggct gataggtcac tactagaaat tcatcatcac catatcttgc ttttatattt
      781 cggtagtacc ttaaatctaa gtcatcttct aggagcaagg aatcagaaga tgcatccaat
      841 tggaaatcat ctaaccaagt agagaaaaat cctccaatca aagctaaaat catcaatacc
      901 gtcttggggt atttaactat ggctttaaga caattattta ctatttgttg cattgctgtt
      961 cttttattat ccaactaaaa gttaaatcaa tcgataaact ctagtttgac tctaaatttt
     1021 agtgcatcat acgtatttag tcaggaaata atattctttt ttattttacg gataaaaaat
     1081 caaaaaatgg caatcaaatc agaccaatgg ataaaaaaaa tggctgaagg tcatagcatg
     1141 attgaacctt ttgttgaaca ccaagtcaga cataataacg gaaatgattt gatttcctat
     1201 gggacttcaa gttatggcta tgatgtacga tgcgcaaatg aatttaaaat ttttaccaat
     1261 attaattctg caatcgttga tcctaaatct tttgacgata ctagctttgt cgatctgaat
     1321 tccgatatct gtgttattcc acccaattca ttcgcactgg ccagaaccat tgaatttttc
     1381 agaattccaa gaaatatctt gactatatgt ctgggtaaat ctacctatgc tcggtgtggc
     1441 attatcgtta atgtcacacc tttggagcct gaatgggaag gtcatgtgac ccttgagttt
     1501 tcaaatacca cacccttgcc ggcaaaaatc tatgccaatg aaggggttgc tcagatgatt
     1561 tttcttgaat ccgatgaagt ttgccaagaa tcctacaagg atagaaaagg gaagtatcaa
     1621 ggtcaattag gggttacttt agctaaaact taatcactct atcgttctag ctttagtgat
     1681 tggcttgcca tttaaaatgg cctgattatc aatcattctt aagacccctt cagcaaacat
     1741 tttaattgcc attgggtaaa tgatgtattc tccttgttga accctctttg ctaatgagcc
     1801 agggttatca tggggtttaa ttttagttct gtattgaagg acaattggtc ctgaatctaa
     1861 ttcaggtatg acaaagtgta cagttgaacc gtggtactcg tcagtagatt ctagaactct
     1921 atcatatgta tttagacctt gatatttcgg taatagtgaa ggatgaatat tcaaaatttt
     1981 tcctttaaat tgatgacaaa actcatctga gagaatcctc atatagcccg ctaaaacgat
     2041 taactctgga ttactttctt taattctttg tactacaatt ttgttaaaaa caaatttgtc
     2101 aggataacta ccgtagtcaa taatttgagt cttaatgtta gctttttctg ctcgttttaa
     2161 gccataagca ttgggattat cagagacgac caaagtaatt tttatgggca attgatgttg
     2221 gtcgacttcg tcaataattg cttgtaaatt tgtccctgat cctgaaatca aaacaacagt
     2281 cgatattctt gagtccattc cagctttctt catttaacca ttacttgccc cgtaccattt
     2341 tcaatgtaac caatctgagt tgattgataa cccaatttta aaaatgtttc cataatttct
     2401 gatgcataat tttttttgat gatgactacc atcccaattc cacaattgaa tgttttgaac
     2461 atttcatctt cacttatttt tccttcgttc tgtatccagt taaaaacatc aggttgagtc
     2521 cagctacttg tatcaataaa tgcattgcag tgattaggaa gaatacgttc tatattctct
     2581 ttaaaaccac cgcctgtaat gtgggccatt ccattgatcg ggaatttttt aattatattg
     2641 agaattggct tgacataaat agtcgtcggt tcaatgagtg catcacccag tgttgcatcc
     2701 ttgagtctac tttccagatt ggattgttga atatcaataa tttttcgaat caaagaatat
     2761 ccattagaat gtggtccaga tgaagacatt ccaataacca tattgcctac ttcaacgctg
     2821 ctgccatcga tgatgttttc aaaatcaaca acgcctacac aaaacccagc caaatcaaag
     2881 tcaccggatt gatagatgcc aggcatttct gctgtttctc caccaattaa tgccatgtta
     2941 gattctttac aaccctgagc tatgccttga ataactcttg aagccattgc aacttccaat
     3001 ttagaggttg cataatagtc aagaaagaag agtggttttg ctccttggac aatgacatca
     3061 ttagcacaca tagcaaccaa atcaatacca atcaaatgct ctttacccaa tatttgagct
     3121 aattttagct tggttcctac cccgtctgta cttgaaacta gtattgggcg tttataatct
     3181 tttacgtgga gctcaaataa tcccccaaac ccaccaatat tagataatac agaatcgcta
     3241 tatgtatctt gtgcaagagg tttaataact gacaccactt cgtttccagt ttctatgtct
     3301 actccggaat ctgcgtaagt tagtgacttg tccttaatca ttacacaaca tcagtttttg
     3361 gatcgaaaat atatttttag agctataata aaacgcaatt gtatactaac atcaacaaaa
     3421 taattattct tatgactcgt tctaaatcag tatggagttt tagttgaata cttcaaatat
     3481 tccaaatctg atttgtcttg tacgcattgc gcttgttgtg ccaattgttc agttcatggt
     3541 ccagactgaa tttttgttat cactcttatt aattgcgatt gcaggtcttt ctgatatttt
     3601 ggatggctac cttgctaaaa gaaataattg gcggaccaag ttgggaacct tgctggatcc
     3661 agtagcagat aaaatacttt tggtttcact cttcataact ctttttagaa tggagttgat
     3721 acccttttgg cttaccttga tcgttattct acgagatgtc atgattgttt ttggcttagg
     3781 attgtatcga tattttgtag atattcctga gatgaatcca actcgtatca gtaagtttaa
     3841 tacttttaca caactggcat ttgttttgtt tgttattgct taccaaatta tcccatttcc
     3901 ttttgaatat ctctcaatgc tctttggagc cttggtttct gtaacttcta ttgttagcgg
     3961 tttagattat tggattagtt ggagtattct ggcaaaaaag aaaatttacc aaaatgggga
     4021 ttaagtggag cagttattac ttccagtatc aatctctgaa catatggttt ttgatagttt
     4081 ctatcctggg ccaaatattg aaatatacca atcaataaaa atagattccc agaaattatt
     4141 atggattgct ggtgctaaag gatccgggaa aacccatttg ttacaagcag cactccatca
     4201 atgcttttct gtttcaaaaa aaggattgta tattccaatg cgtgatttta aagagtttaa
     4261 tccagaaatt ttagataatc tagatcaact cgatctcata tgtattgacg atattgagtg
     4321 tgttctaggg aagagaatct gggaagaaaa actgatgggt ctttatgaaa aactacaaac
     4381 aacgaaagca caattggttg ttggatcctt tgagtcaccg aaaggtactc atttttattt
     4441 acctgatctt gcttctcggt ttgctatgtt tatggttcat aaactggagt tacttgctga
     4501 gtctgaaaca atatctgcaa ttcagttaca tgcaaattcc agaggatttg acctgcctga
     4561 tgattgtgca aaatatctaa tcagacgtgt tcaaagagat gtgagttcat tagttgaaat
     4621 aattcagcta ctggattatg aatcgctatc aaaaaaaaga atattgacta ttccgtttat
     4681 aaaaagcatt ttaaatcttg gataaaaaag caaatattga atcacgcctt gacctgcata
     4741 tgaggtcttg ataggtttag ggatgttcaa gtcttttatt accatgaatc attcaatatc
     4801 aggtcctata aaaattctaa gcgttgcctt ggtttcaact ttacttttat cttgtgcaac
     4861 tttaccacct ttacaggaaa tgagtaatgc caggcagact attgcggcag caaaagaatt
     4921 cagtcctaat gcggctactg ataaaaaaat cttggaagct gaacgtttgc ttgaacgagc
     4981 agaaagacgg attgaggtga ctttatatga acaggccaga ctcgatgcac ttcgagctcg
     5041 aaatgaagca attgaatacc ttgaacaagc aattgcacaa agagctatat cttcttcacc
     5101 cgattaaaga aaataggact ttaagagcta aatttttcaa gaaaagcagg aagctttgca
     5161 aaaggtatac tggtgttact cttttctcct ctggctttaa attctatgcc ttgactcttc
     5221 agacctttct tgcttaaaac cagttgtttt gggatcccta ttaaatccgc atcggcaaat
     5281 tttactccag ctctctcatc cctatcgtca agtattactt cgagaccata ttctaaaagt
     5341 tgctgataaa tttcatatgc ttttttcgag acttcatctt ccaaattttg attcaatgat
     5401 atgatagaaa tttggaaagg ggttatagaa tcaggccaaa taatcccgga ttggtcatta
     5461 ttttgctcaa tagcagcagc aactatccta gagacgccga ttccataaca acccattaat
     5521 ggatgtgtag agttaccttt ttcattggaa accacgcaat tcatatgttt gctgtattta
     5581 tcacctagtt ggaaaatatg gccaacttcg atgcctcgtg cgtaggaaag ttgtcctcca
     5641 ttgggagctg tgtccccatc gatcatttca acattggcag caaactcttc ttcatcacaa
     5701 tatccgatct tgtcttctcc agagtcagct agaacaacat attcatgtga aagagaacca
     5761 ccaatttctc cgcttccagc ccgaactcta cgataatcca atccaatcct agtgaaaata
     5821 gtatcgtagg ttctacccat attttcatat tcttggtcaa gcgatttctg atccatatga
     5881 aatgagtaag catctttcat gagaaactct ctggctctca ttacgccgaa tctaggtctt
     5941 atttcatctc taaacttagt ttgaatttga tagtaattga ttggcagttg gcggtatgtt
     6001 tttagttcat ttctaacaat gtc
//
LOCUS       DASCTH010000138         4883 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000187739, whole genome shotgun sequence.
ACCESSION   DASCTH010000138 DASCTH010000000
VERSION     DASCTH010000138.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000187739"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="QGH35_02975"
     CDS             complement(<1..824)
                     /locus_tag="QGH35_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HJL81530.1"
                     /translation="MNIIYWIVLLATFAGIHFLGSRTELKLWARVVIALVLGVIIGFI
                     FGDLAQTSKWIGDLFVRLIRMLVIPLIFTSLVAGVVSMGDPKRLGSIGLKTIGLYLIT
                     TLFAIIIGLSLGSVFQPGQGMDLTGVEPFTSGSTSTSVTDQLFGIIPENPISSLANGQ
                     VLPVIFFSILLGIGIIMGGEKTKSLGDVFSSAAEAVLKIAYLVMQLAPYGVLSLIAWV
                     SGTLGMEALQTMLKLTTLLYCGCILHMLLVYGGALKLIIRLPLIRFFRGIADAQAVA"
     gene            complement(871..2589)
                     /gene="ggt"
                     /locus_tag="QGH35_02980"
     CDS             complement(871..2589)
                     /gene="ggt"
                     /locus_tag="QGH35_02980"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJL81531.1"
                     /translation="MFYRFLFVLIISFNAWGQYSYIDYPSPYHPTLSQRGMVVSQNID
                     SSKIGIEILNKGGNAIDAAVAVGFSLAVTLPRAGNLGGAGFMLVYLKEKDEIYAIDYL
                     GSSPKHATTRDLFGVELPKGYKKADRDLVRYGYKASTIPGTVAGLIEAHSNFGRLPLK
                     EVLQPAIEQAKNGIKVSYDLHMALTDSSQLKNDPESRKIFFEGGSAMKENTILKRPDL
                     AQTLERISEEGAKGFYQGETAKKIVEAMKKNKGYISLEDLQNYKPKFSDPILTVYRGN
                     RIYAPRPPSGGAIVVLDALNILENFNLAKYKSNSSATYHLLAEALRRGHVDRSRYIGD
                     PDFSDVPVGKIISKKRARELAKTISFKSATSTKSLSPKFYFEESKDTTHFSIIDEDGN
                     AVSNTYTLGYSFGSGVTIPGTGILMNNHMNNFAYRYGDKSVRGRAASLANRFDSAKRP
                     MTTMTPVIAFNNKGKLMLITGSPGGVNIPAAVLRVITGVIDFGLHIGEATMLPRISKS
                     WPEEDLDYESTASSDVMAILEKIGHKTESSNTMGSTQSIHIKDGLNQGYADLRRPNAG
                     VAVQSD"
     gene            complement(2738..>4883)
                     /locus_tag="QGH35_02985"
     CDS             complement(2738..>4883)
                     /locus_tag="QGH35_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81532.1"
                     /translation="SSEHGSSHPYNHVFTDSNGEFEVFPLGDPRCNNRSSQNGEVFDT
                     GYGTCIAQDGNGVERFNLWGDTDYRSELKRENVFIFINHELDNGNEAFTEIGFYESAS
                     NLTRHPSYAFSSSKHRVGPDNYWLNQMTLADGTALFAGKELYIDNYRYAEIYRKVDVK
                     KRTHRFLQGLRGSSGDWDWETAFLTSKATSDDVTGNRLSNNLIKAALWDSTPAAYNPF
                     SAGVDSNLDQAVIDVYRKGSSELRMLDFKASNNEIADLPAGPLGLLVGFEYRYEKVVD
                     DRDPRLDGTIIYTDYEGDTYPLVSDVLNSSPTGDVSGEHRVKSIFTELQIPIAATANA
                     QIALRYEDSTDVESAMVGKFAVGWNITDWLLFRGSVSTAYRAPNIIQVNEKIVVRTGT
                     RNDYSFYRLQQANGISTSSSTLDSRYSMQRQASGAENLKSEESDNTSVGFVITPVDGL
                     VITADYWTIEKENTIGLFGRNNHTVNDMMLRWANGTNNCDTFVGNPAVVREAPDSGDI
                     EDGYFATAGVCPFGVIKYVADEYLNLATRTIEGYDIGIYYDVDTDFGDFGIRYIGSYI
                     DKFEQVPGGEFSAMATQQASGAIPADIPIDGFGDLLGTNGNYDEKHRLRLSWNKGPWG
                     TTLTGLRKGSFYQSSLTLSDGTRFVIPSMTVMDLTVDYRFNLKNSAARIRFAVKNLAD
                     ERAPTADRYYGFFADAHQDLGRNFYVDFRVSL"
BASE COUNT         1435 a         1093 c          912 g         1443 t
ORIGIN      
        1 gcaacagcct gggcatctgc aatgcctcta aaaaatctga tcaaaggtaa tcgaataatc
       61 aatttcaagg cacctccata gacaaggagc atatggagaa tacaaccaca ataaagaagg
      121 gtggttagtt taagcatggt ttgcaaagct tccataccca gtgtacctga cacccaagcg
      181 attaagctca aaacaccata gggtgctagt tgcataacga ggtaggcaat ttttagcaca
      241 gcttctgctg ctgaagaaaa aacatccccc agagattttg ttttttctcc acccattatg
      301 attcctatac ccaataaaat agaaaaaaag ataactggta atacctgccc attcgctaat
      361 gaggaaatcg gattctctgg aatgatccca aacaattgat cggtaacact tgttgacgta
      421 cttcctgacg taaagggctc aacaccggta agatccatac cctgccctgg ttgaaaaact
      481 gaacctaagc tcaagccgat aattattgca aacagtgtgg taattaaata caaaccaatg
      541 gtcttgagtc caatggaacc caatcgcttt gggtctccca tggaaactac accggccacc
      601 aaagaggtaa atatcaaggg aatcaccaac attctaataa ggcggacaaa taaatcgcca
      661 atccattttg atgtttgggc tagatctcca aaaataaatc caatgattac accaagaacg
      721 agagcaataa ctactcgtgc ccatagtttc aattccgttc tagatcccaa aaagtgaata
      781 cccgcaaagg ttgcaagtaa aacgatccaa taaataatat tcattcccat agcaagacta
      841 ttctacagta aatactgagt gactagttta ttaatcactt tgtacagcta ctccagcatt
      901 gggtcttctc aagtctgcat agccttgatt caggccatct ttaatatgga tgctttgggt
      961 gctccccatg gtattggagg actccgtttt atgaccaatt ttttctaaaa tagccatcac
     1021 atctgagctg gcagtcgact cgtaatctaa atcctcttct ggccagcttt tgcttattct
     1081 tggcaacatc gtggcttccc ctatatggag accaaaatca atcactccgg taatcaccct
     1141 cagcacagca gcaggtatat tgactccgcc aggtgagcct gtgattaaca ttaacttgcc
     1201 tttgttgtta aaagcaataa ccggtgtcat cgtggtcatc ggtcttttag cagagtcaaa
     1261 tctgttggcc agactggctg ctctgcccct taccgattta tccccatagc gataagcaaa
     1321 attattcata tgattgttca tcaaaatccc agtaccagga atggttacac cggatccaaa
     1381 cgaataaccc aaggtgtaag tattggacac tgcatttccg tcttcatcaa taatggaaaa
     1441 atgggtagta tccttgcttt cctcaaaata aaattttgga gaaagtgact tagtggaagt
     1501 agcggactta aaactgattg tttttgccaa ttctcttgct ctttttttcg aaatgatctt
     1561 ccctactgga acatcagaga aatcaggatc accgatatac cttgagcggt ctacatgccc
     1621 tcttctgagc gcttctgcca agagatggta agttgcagaa gagtttgatt tatatttagc
     1681 cagattaaag ttttcaagaa tattgagtgc atccagaacg actattgcac ctcctgatgg
     1741 agggcgtggg gcataaatac gatttcccct atacaccgtt aggataggat cagagaattt
     1801 tggtttataa ttttgcagat cttccaatga aatataacct ttattttttt tcatggcttc
     1861 cacgatcttt ttagccgtct ccccttgata gaaacccttg gctccctctt ctgatattct
     1921 ttccaatgtt tgagctaaat cgggtctttt gagtattgtg ttttctttca ttgcagaccc
     1981 cccctcaaaa aatatttttc tcgattcagg atcatttttg agctgtgatg aatctgtcag
     2041 tgccatatgc agatcatagg aaaccttaat gccattcttg gcttgttcaa tggcaggctg
     2101 tagtacttcc tttaggggta aacgaccaaa attagaatgg gcttcaatca gaccggcgac
     2161 ggtgcctgga atggtggatg ctttatatcc ataacgaaca agatctctat cagctttttt
     2221 gtaacccttt ggtaattcaa caccaaataa atctctggta gtggcatgct tgggtgatga
     2281 tccaagataa tctattgcat aaatttcatc tttttctttc agatagacca acataaaccc
     2341 agcgccccct aaattgccag ctctgggcaa tgttactgcc aatgaaaatc caacagccac
     2401 cgctgcatcg attgcattac ctcctttatt gaggatctct attccaatct tggaagaatc
     2461 gatattttgt gaaacaacca tgcctctttg acttaatgtc ggatgatagg gtgacgggta
     2521 gtcaatatag gaatactgac cccacgcatt gaaagaaata attagaacaa ataaaaatct
     2581 ataaaacatc aattcctacc aataataact gaactggaag tgtaacttgc tctttttctc
     2641 ttttctacca gttgtctcta aagctcactt cgtcaatggc atttatacta taaaaaaaag
     2701 ggagcctaag ctcccttttt ttgttttgct aaactactta gagagatacc ctgaagtcta
     2761 cgtaaaagtt tcttcctaag tcttggtgag catctgcaaa aaagccatag tatctgtctg
     2821 cagttggtgc tctctcatcg gctaaattct taacagcaaa tcttatcctt gctgcagaat
     2881 tcttcaaatt aaaacggtaa tcgacggtaa gatccattac tgtcatggaa ggaatcacaa
     2941 aacgagtacc atcagaaagc gtgaggctac tctggtaaaa agaacctttt ctcaaaccag
     3001 taagtgtagt gccccatgga cctttgttcc atgaaagtcg tagtctgtgt ttctcgtcgt
     3061 aattgccatt agttccaagc aagtcaccaa acccatcgat tggtatatct gctggaattg
     3121 cacctgatgc ttgttgggtt gccatcgcag agaattctcc tccaggaact tgctcgaact
     3181 tatctatgta gctgccaata tagcgaattc caaaatcacc gaaatcagta tcaacgtcat
     3241 agtaaatccc gatgtcgtat ccttctatgg ttcttgtggc taagtttaga tactcatcag
     3301 caacatactt aataacacca aacggacata cgccagcagt agcaaaatag ccatcttcaa
     3361 tatcacctga atcaggtgct tctctgacaa ccgctgggtt gccaacaaaa gtgtcacaat
     3421 tgttcgtacc gtttgcccat ctcaacatca tgtcattcac cgtatggtta tttctgccaa
     3481 acaaacctat agtgttttct ttttcaatag tccaataatc agctgtgatt accaagccat
     3541 caacaggagt aataacaaat ccaacggagg tattatccga ctcttcagac ttaagatttt
     3601 cagctcctga tgcttgtctc tgcatggaat aacgagaatc caaagttgag ctggatgtgg
     3661 atatcccatt tgcttgttgt aatcgataga agctgtaatc atttctggtt ccagttctca
     3721 caactatttt ttcattgacc tgaattatgt ttggagccct gtaggctgtt gaaactgaac
     3781 ctctgaataa caaccaatca gtgatattcc acccgacagc aaattttcca accatagcgc
     3841 tttccacgtc tgtggaatct tcgtatctta gagcaatctg tgcattggcg gttgctgcta
     3901 taggtatttg taattcagta aaaatggatt ttacacggtg ttcacccgaa acatcacctg
     3961 taggacttga gttcaaaaca tctgaaacca ggggataggt atcaccttcg taatctgtgt
     4021 aaatgatggt tccatcgagt ctgggatccc tatcatccac aaccttttcg tatctatact
     4081 caaaacctac taatagaccc agaggaccag caggaaggtc tgcgatttca ttatttgaag
     4141 ctttaaaatc tagcattcta agctcactcg aaccttttct atagacatca ataaccgctt
     4201 gatcaagatt tgaatccaca ccagcactaa aagggttata ggcagctggg gtagaatccc
     4261 agagtgctgc tttgattaga ttgtttgaca atctattgcc agtaacatca tctgaggtcg
     4321 ctttggatgt caaaaaagct gtttcccaat cccagtctcc actcgaacct ctgaggccct
     4381 gtaaaaacct gtgggtgcgt ttcttgacat caacttttct atagatttcg gcatagcggt
     4441 agttatcaat atacaactcc tttccagcaa aaagtgccgt accgtcagca agtgtcattt
     4501 gattcagcca ataattatca ggaccgactc tgtgctttga ggaagagaaa gcatatgagg
     4561 gatgccgagt tagatttgaa gcagactcat agaatccaat ctcagtgaat gcttcgttgc
     4621 cgttgtcaag ttcatgattg atgaagataa aaacgttttc cctttttagt tctgatctgt
     4681 aatccgtatc tccccatagg ttgaaccgtt ctacaccatt gccatcttgt gcaatacaag
     4741 taccataacc ggtgtcaaat acttccccat tctggcttga acgattgttg caacgtggat
     4801 ctccgagtgg aaaaacttcg aactcaccat tcgaatcggt aaaaacatga ttgtatggat
     4861 gactgctacc gtgttcactg ctt
//
LOCUS       DASCTH010000139         1744 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000190281, whole genome shotgun sequence.
ACCESSION   DASCTH010000139 DASCTH010000000
VERSION     DASCTH010000139.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000190281"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /gene="purD"
                     /locus_tag="QGH35_02990"
     CDS             <1..968
                     /gene="purD"
                     /locus_tag="QGH35_02990"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HJL81533.1"
                     /translation="KSFMQKYRIPTATYQSFTDPHLAKEFLKNSTYPMVIKANGLAAG
                     KGVCIVNNFTMAEKSVDEIMTDHKFGNAGATIVVEEFLEGEEVSFIAMVDGVNILPLA
                     TSQDHKAIKDGDTGPYTGGMGAYSPVPFVDADLQRKIMDQIMLPTVHGMINEGIPYRG
                     FLYAGLMISDQNEPKVLEFNCRFGDPETQPIMMRLSSDLVELIQACFDETIESFPIKW
                     DKRTALGVIMASLGYPEKYETNMEINGLDGTDLADPDLKIFHSGTTIINDQIVTNGGR
                     VLCVTALGRDAKQAQKVAYDAVNKITWNGVYYRRDIGDKAIKIMN"
     gene            complement(965..>1744)
                     /gene="rho"
                     /locus_tag="QGH35_02995"
     CDS             complement(965..>1744)
                     /gene="rho"
                     /locus_tag="QGH35_02995"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HJL81534.1"
                     /translation="RIIDLSAPIGKGQRGLIVSPPKAGKTILLQNIASSITANYPDVH
                     LIVLLIDERPEEVTDMQRTVRGEVVSSTFDEPASRHVQVADMVIEKAKRIAEHKQDVV
                     ILLDSITRLARAYNTVAPSSGKVLTGGVDANALQKPKRFFGAARNIEEGGSLTIIATA
                     LVDTGSRMDEVIFEEFKGTGNMEIHLDRRISEKRVYPAIHINRSGTRREDLLMDPDEL
                     QKMWVLRKVVHDMDEIAAIEFLINKMKDTKTNAEFFESMKR"
BASE COUNT          517 a          361 c          339 g          527 t
ORIGIN      
        1 caaaaagttt tatgcaaaaa tacaggatcc cgacagcaac ttatcaatcc tttacagacc
       61 cccatttggc aaaagaattc ttaaaaaact ccacctatcc aatggtaatc aaagcaaatg
      121 gcttagctgc aggcaaaggg gtatgtattg taaacaattt tacaatggca gaaaaaagtg
      181 ttgatgagat catgacagat cataaatttg gaaacgcagg agcaacgatt gttgttgaag
      241 aattcttgga aggtgaggaa gtaagcttca ttgccatggt agatggggta aatatcctac
      301 ctctagctac ctcacaggat cataaggcaa tcaaggatgg agacactggt ccatataccg
      361 gtggaatggg agcttattcc ccagtccctt ttgttgatgc tgatcttcag agaaaaatta
      421 tggatcagat catgctgcca actgttcatg gtatgatcaa tgaaggcatt ccatacagag
      481 ggtttctcta tgctggtcta atgatttcag atcaaaatga gcctaaagta ctggagttta
      541 attgtcgctt tggcgatcct gaaacacaac caattatgat gcgactctca tcggatttgg
      601 ttgaactcat tcaagcttgt tttgatgaga ccattgaatc gtttcctatt aaatgggata
      661 agcgaactgc tctgggtgtt atcatggcca gtctaggcta tcctgagaaa tatgaaacta
      721 acatggaaat taatggctta gatggaacgg atctagcaga tcctgattta aaaatcttcc
      781 actcaggaac aacaatcatt aatgatcaaa ttgttactaa tggcggcagg gtactgtgtg
      841 ttactgctct aggtagagat gctaaacaag cacaaaaggt tgcttacgat gcagttaata
      901 agattacttg gaatggtgta tattatcgaa gagatatagg agataaggca atcaagataa
      961 tgaattagcg tttcatcgat tcaaaaaatt cagcattggt cttggtatcc ttcatcttat
     1021 tgatcaagaa ttcaatggct gctatctcat ccatatcgtg tactactttt cttaacaccc
     1081 acattttctg cagttcatca ggatccatca ataaatcctc cctacgcgtt cctgaacgat
     1141 tgatgtgaat cgcagggtaa acccttttct ctgaaattct tctatccaaa tgaatttcca
     1201 tattgcctgt gcctttaaat tcctcaaaaa tgacttcgtc cattcttgat cccgtatcaa
     1261 ccaatgctgt ggcaataatt gtcaaactac caccttcttc aatatttctc gctgctccaa
     1321 aaaacctttt tggcttttgc agagcattag catctacccc tcctgtcaag actttacctg
     1381 acgagggtgc aaccgtatta taggctcttg ctagccttgt tatggagtct agtaaaataa
     1441 ccacatcttg tttgtgttca gcaatacgct ttgccttctc aatcaccata tcagctactt
     1501 gaacgtgacg cgatgctggt tcatcaaagg tactggaaac cacctctcct ctaaccgttc
     1561 tttgcatgtc ggtcacttct tctggtcttt catcaatcaa taaaacaatc agatgcacat
     1621 ctgggtagtt agctgtaatg gaggatgcaa tattttgaag caagattgtc ttaccagcct
     1681 ttggtgggga tacaattagc cctctctgcc cttttccaat tggcgcagat agatcaatga
     1741 ttct
//
LOCUS       DASCTH010000140         2511 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000191772, whole genome shotgun sequence.
ACCESSION   DASCTH010000140 DASCTH010000000
VERSION     DASCTH010000140.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000191772"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="QGH35_03000"
     CDS             complement(<1..209)
                     /locus_tag="QGH35_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81535.1"
                     /translation="MKNEQQKIENAKEFVENNFPWWLAELLEEANGSAKLGICPPLVD
                     HLDINYLIDVMDREPNLEKVIGNIIT"
     gene            607..682
                     /locus_tag="QGH35_03005"
     tRNA            607..682
                     /locus_tag="QGH35_03005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:640..642,aa:Gly,seq:gcc)
     gene            715..1149
                     /locus_tag="QGH35_03010"
     CDS             715..1149
                     /locus_tag="QGH35_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020029247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81536.1"
                     /translation="MRYLFLIPILFMNLAFAEIYEDYDLEENVIELTVIAVEPNYVDN
                     YLVNLKKTWVRSADIQKELGHIVDYGVYTSNVANSPNVWISVTYENMAAMQPSEEKYN
                     QVNKIIEERFSESEEEYDAISKGYEEIRKMVDYQIINKVIFK"
     gene            1230..>2511
                     /locus_tag="QGH35_03015"
     CDS             1230..>2511
                     /locus_tag="QGH35_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HJL81537.1"
                     /translation="MKLSKSSIVIIVALGIFLIYMFGQSESSLEIVDFSMDKPEAPTT
                     SIASNENRNPYYGDLHVHTKYSFDAYVFGITATPDDAYRYAQGEGIKHPLGYEMKLRE
                     PLDFYAVTDHGIYMGMMEAYADTSTKISQNSWAIPIHNINSPENLNDGSFATRANLFS
                     SLIRGIIINPYPYWHPKVIRAWFTKNTALALKSFDYDVHKSAWADVARAAEEHNDPGK
                     FTSFIGYEFTSSTNVEGGNLHRNVIFNSSKAPIRPWSRIDSINPEDLWTWMDKLRDKG
                     VDSLAMPHNSNGSNGQMFEMETFRSYPIDTIYSEKRMRNEPVVEITQVKGTSETHPLL
                     SPDDEWADFAIMDKRIGSRPPTYSMAPGGYVRDAYLRGLSLSWTRQGNPYKFGLIGSS
                     DSHTGAGAYDENNYWSKVGLLDGTAEARGAVPLTA"
BASE COUNT          796 a          437 c          512 g          766 t
ORIGIN      
        1 gtaatgatat taccaataac tttctcgaga tttggttctc tatccataac atctattaag
       61 tagttaatgt ctagatgatc gacaagtggt ggacagatcc ctaactttgc agatccgtta
      121 gcctcttcta atagttctgc taaccaccat gggaagttgt tctccacgaa ctctttagca
      181 ttctcaatct tttgttgttc atttttcata attattctcc tttgttgaaa tcggcacttt
      241 tagttatatt ccgagtgaat gttttttgtg tcgttcactt ctgctgacga ccaacaaaac
      301 atgaacgagg agaactacta aaccttgtaa agtttttaaa gtaatctggt ttaagacagg
      361 ttgataacct aaaatcagaa caaaatgtga gtggtctata taatctatgg agtaggggct
      421 aaggcgacct gcgtaggtgc aagagcaata aaatttctta tgttgataac aaattaattt
      481 ctgtatactt ttcatagaat agatacacga tttactttgt ttttacatca gaatgtgact
      541 tatccgtgac ttaagttagc acgcataagt agttaatttt ctgaaagata gtgtagataa
      601 aggtttgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
      661 tcgaatctcg tttcccgctc cagtttttta aaaaagaaaa tataggagaa aactatgaga
      721 tatttatttt taattccaat tcttttcatg aatctggcat ttgctgaaat ctatgaagat
      781 tatgatttag aagaaaatgt aattgaactg actgtaattg cagttgaacc aaattatgtt
      841 gataattact tggtcaatct caagaaaact tgggttagaa gtgcagatat ccagaaagaa
      901 ttgggccata ttgttgacta tggtgtttac acatcaaatg ttgctaatag cccaaatgta
      961 tggatttcag taacttatga gaatatggca gcaatgcaac caagcgaaga aaaatataac
     1021 caggtcaaca aaatcatcga agaacgcttt agtgaatctg aagaagaata cgatgcaatt
     1081 tcgaaaggat acgaagaaat tagaaaaatg gtagattacc aaatcatcaa taaagttatt
     1141 ttcaaataaa tctttttgaa tcagagggct tctgcaaagg agcctctcta aaattatata
     1201 gcttggcaaa aaatttgtag gaggaataga tgaaactcag taaatcttca attgttatta
     1261 tagttgctct tggcattttc ttaatatata tgtttggtca aagtgagtcc tcactagaga
     1321 tagtggattt ttctatggat aagcccgagg caccaaccac ctctattgcc tctaatgaga
     1381 atagaaaccc atattatggg gatcttcatg tgcacacaaa atactctttt gatgcctatg
     1441 tcttcgggat cacagcgaca ccagatgatg cgtatcgtta tgctcaaggt gaaggtatta
     1501 agcacccttt gggttatgaa atgaagctac gtgaaccctt ggatttttac gctgtgacag
     1561 accatggtat ttatatggga atgatggaag cctatgctga tacctccacc aaaatatctc
     1621 aaaactcctg ggccatacct attcacaata ttaatagtcc agagaatctt aatgatgggt
     1681 cctttgctac gagagctaat ctatttagct cactgatacg tggaatcatc attaatccat
     1741 atccatattg gcatcccaag gtaataagag cctggtttac taaaaatact gcactggccc
     1801 tcaaatcgtt tgattatgac gtgcataaat ctgcttgggc tgatgttgcc agagctgcag
     1861 aagaacataa tgatccagga aaatttactt catttattgg ctatgaattc acttcttcaa
     1921 ctaatgttga gggtgggaac cttcacagga atgtcatctt taattcttcg aaagccccga
     1981 ttagaccttg gtcaagaatt gattctatca accctgaaga cttgtggact tggatggata
     2041 aattaaggga caagggagta gattctttgg ccatgccaca caatagcaat ggctccaatg
     2101 gtcagatgtt tgaaatggag acatttagaa gttatccaat agacactatc tactctgaaa
     2161 aaagaatgag aaatgaacca gttgttgaaa ttacccaagt caaaggaacc tcggaaactc
     2221 acccattact ttcgcctgat gacgagtggg cagactttgc aattatggat aaaagaattg
     2281 gcagtaggcc tccaacatac agcatggctc caggtggtta tgtgagagat gcttatttaa
     2341 gaggtctaag tctgtcgtgg acacgccaag gaaatcctta taagtttggc ctcataggtt
     2401 caagtgattc tcatactgga gcaggggcat atgatgagaa taattattgg tctaaagtag
     2461 gtctgctgga tggcactgct gaggcaagag gggccgtacc tttaactgca g
//
LOCUS       DASCTH010000141         2092 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000192883, whole genome shotgun sequence.
ACCESSION   DASCTH010000141 DASCTH010000000
VERSION     DASCTH010000141.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000192883"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="QGH35_03020"
     CDS             <1..339
                     /locus_tag="QGH35_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HJL81538.1"
                     /translation="VIEHTQIIGKLGILEWIFVTPSAHRVHHGYSPKYLDKNYGNLLI
                     IWDRIFGTYVEEDEPVLFGLSNNVNTFNPLKITLFSWVSYIQDFKESQSITDKILSFW
                     GTPEWSPRTQ"
     gene            complement(366..1319)
                     /gene="cysK"
                     /locus_tag="QGH35_03025"
     CDS             complement(366..1319)
                     /gene="cysK"
                     /locus_tag="QGH35_03025"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HJL81539.1"
                     /translation="MIYNNIIETIGNTPIVRINSMGPENQNIYVKCEAFNPLASVKDR
                     LAIAIIADAEASGELKPGQTVVEATSGNTGISLAMVCAAKGYPFVATMVETFSIERRK
                     IMKALGAKVILTPAAERGTGMVLLAEKLAKKHGWFLARQFENPANPAYHRNTTAPEIL
                     RDFGDKDLNYFVSGWGTGGTITGVGEVLKAARPNIKIIGAEPETASLLSGKEWQPHKI
                     QGWTPDFVPKVLDPQHIDQIVTVNEDVSIEMAKELASKEGIFCGISAGATMQAALQIA
                     HDAPDNSTFCVMLPDTGERYFSSPLFADINEDSDDEWLATQ"
     gene            1479..>2092
                     /locus_tag="QGH35_03030"
     CDS             1479..>2092
                     /locus_tag="QGH35_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HJL81540.1"
                     /translation="MKHDARKYCHIKGPFTLKNGGVLESPIIAFETWGTLNASKDNGV
                     LIFTGLSPSAHAASSKNDPSKGWWEKMIGPNKPIDTERYFVICANSLGSCFGSTGPAS
                     IDPVTNKTFGLTFPKLALEDIGCATRKILDQLEINSLQAIIGPSMGGMTALGFLVENP
                     DICKQLLLISTASTSRPFSIALRSLQRLMITSDPKWKSGNYDLN"
BASE COUNT          600 a          441 c          402 g          649 t
ORIGIN      
        1 gtcattgaac acactcaaat cataggcaaa ttaggcatat tggaatggat ttttgtcaca
       61 ccatcagcac atcgagtaca tcatggatat agtccaaaat atttggataa aaattacggc
      121 aatttattga tcatatggga ccgaatattt ggtacctacg ttgaggagga tgagccagtt
      181 ttgtttggct tgagcaataa tgtaaatact ttcaacccac taaaaattac actttttagc
      241 tgggtttcat atatccaaga cttcaaagaa tcacaaagca ttactgataa aattctctcc
      301 ttctggggca cgccagaatg gagtcctcga actcagtaaa tcaaaatcat cgtctcaata
      361 aatagttatt gtgtagcaag ccattcatca tcactgtctt cattaatatc tgcaaacaat
      421 ggtgaggaaa aataacgctc tcctgtatcc ggcaacatga cacagaatgt actattgtct
      481 ggcgcgtcat gagcaatctg caaagctgct tgcatagtgg caccagctga gatgccacag
      541 aaaatgcctt ccttgcttgc aagttctttg gccatttcaa tggatacatc ctcgttaacg
      601 gtgactattt ggtcaatgtg ttgtgggtct aagaccttag gaacaaagtc aggcgtccat
      661 ccctgaatct tgtgtggctg ccactccttg ccagatagta aagaggccgt ttctggttct
      721 gctccaatga tttttatatt gggtcgtgcg gcttttaata cttcgcccac accggtgata
      781 gtaccgcctg taccccatcc actaacaaaa taattgaggt ctttatcacc aaaatctctt
      841 aatatttccg gcgctgtggt atttcggtga taagccggat tagctgggtt ctcgaattgt
      901 ctggccaaaa accagccatg ctttttagcg agcttttcag cgagtagtac cattccggta
      961 ccgcgttctg cggccggtgt aagaatcact tttgcaccaa gagctttcat tatctttctt
     1021 cgttctatag agaatgtttc taccattgta gccacgaaag gataaccctt agcagcacaa
     1081 accatagcta gtgagatacc tgtattacct gaagttgcct cgacaacagt ctgccctggt
     1141 tttaattcac cgcttgcttc ggcatctgca ataatagcta tggccaagcg atccttgacc
     1201 gaagcaagag ggttgaacgc ttcgcattta acataaatat tttgattttc tggccccatc
     1261 gagttaattc ttactatcgg tgtattacca atggtttcaa taatattgtt atagatcata
     1321 aaacaccttc tgctattaat tttgttggat tcataaaata ttcggttgct cattctactg
     1381 ataaagtccg tatttactta cactttttag atctaaatat ataactatat gttggatcat
     1441 aacttcaact gataaaggat aatgtatgca aatttactat gaagcatgat gcaagaaaat
     1501 attgtcatat aaagggtcct tttactctta aaaatggagg tgttttagaa tcacccataa
     1561 tcgctttcga aacctggggg actctcaacg cttccaaaga taatggtgtc ctgattttta
     1621 caggcttatc tccctctgcg cacgctgcct catccaaaaa tgacccttcc aaaggctggt
     1681 gggagaagat gattggacct aataaaccga tcgacacaga aagatatttt gtcatttgtg
     1741 ccaattcctt gggcagctgt tttggatcca ccggtccggc cagcatcgat cctgtaacta
     1801 ataaaacctt tggattaacc tttcccaaat tggctttaga ggatattgga tgtgctacaa
     1861 gaaaaattct agaccagttg gaaattaatt ccttacaagc gattataggt ccttcaatgg
     1921 gaggcatgac tgcattgggt tttctagtag aaaatcccga tatttgtaag caattattat
     1981 tgatttcaac tgctagcaca tccagaccct tttctatcgc attgcgatcc ttgcaacgat
     2041 tgatgattac ctctgatcct aaatggaaat ctggtaatta tgatttgaat ga
//
LOCUS       DASCTH010000142         2428 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000194114, whole genome shotgun sequence.
ACCESSION   DASCTH010000142 DASCTH010000000
VERSION     DASCTH010000142.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000194114"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="QGH35_03035"
     CDS             <1..767
                     /locus_tag="QGH35_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81541.1"
                     /translation="VAVLMRTVKEPSRKDKIIIRGQDGQQTDEISVKQTIDFLLARKK
                     TYGWLFLSMACSVLIGYAFLSWMPAMYIRTYNIDIGTIALWQGLVFLIGGPLGATMSG
                     VFGDKLFNKGDNAAHVKLFAYCFIIMAVSASLVPLMPTYQLATLMFLPQIITAAAQTA
                     LAPIAIINITPNQIRGQVTAIYYFVISMTGYTLGPTSVGLLNDYFFKDESMIRYSMTM
                     VSITVGIIGIIAGFKCIKHYRKHITESQTGNYAESH"
     gene            complement(773..1984)
                     /locus_tag="QGH35_03040"
     CDS             complement(773..1984)
                     /locus_tag="QGH35_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HJL81542.1"
                     /translation="MIDSNTGKMFSQDNLPLKGIKVTEFTHMIMGPAVGAILADLGAE
                     VIRIEPVTGDKTRLLPGSGIGFFTSFNRNKRSIALDIKSDQGKDIALKLIQESDVLIE
                     NFRPGTMERLGFGYEDLKPLNNRLIYCSAKGFLQGPYENRTALDEIAQMMGGLAHMTG
                     PIGQPLRAGSSVIDIMGGMFGVIGILAACEQLKTTGRGQKITSALFENVAYLMTQHMA
                     QYAITGEAVPPMPVRKSVWAIYEVFQTKGDEQVFVGVVSDAQWQSFCKAFDLLELLEN
                     SDLNENAGRVEHRDLIIPIITETFKQYTKLELMQKLEETGLPFAPISKPEDLFEDPHL
                     NASDGLVAIDLPEGGQVKLPTLPIEMDGNRFGVRYQPSQPGEHTREILRELDFDDEII
                     DQMVNESVVSE"
     gene            2144..>2428
                     /locus_tag="QGH35_03045"
     CDS             2144..>2428
                     /locus_tag="QGH35_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81543.1"
                     /translation="MPSSISTIAIGVENLNSALDLWVDTFDLELVKKITGLDENLATL
                     WGIDPDNIRTQALVRTPGVEIGQLHLVEFKTPNDPVRKDANSTDLGPKNLD"
BASE COUNT          745 a          537 c          448 g          698 t
ORIGIN      
        1 ttgttgcagt gctgatgaga accgttaaag aaccttcaag aaaagacaaa ataataatca
       61 ggggacaaga cggtcagcag accgatgaaa tttccgtaaa acaaacgatt gattttcttt
      121 tagcaagaaa gaaaacttat ggatggttgt ttctatcgat ggcatgttca gttctgattg
      181 gatatgcatt cttgtcatgg atgcctgcaa tgtacatcag aacatataac atagacatcg
      241 gcacaattgc actttggcaa ggattggtgt ttcttatcgg tggaccactt ggtgcaacca
      301 tgagtggtgt atttggagat aaactattta acaagggtga taatgctgca cacgttaaat
      361 tgttcgcgta ttgcttcatc ataatggccg tcagcgcctc actagtgccc ctgatgccta
      421 cttatcagtt agcaactctg atgtttcttc ctcaaataat tactgcagct gcccagacag
      481 ctcttgcccc cattgcaata atcaacatca ctcctaatca aatcagagga caagtcaccg
      541 ccatctatta ctttgtcatc agcatgacag gttacacact agggcctact agtgtgggac
      601 tactaaacga ttactttttc aaagatgaaa gcatgattcg ctattccatg accatggtat
      661 caatcacagt cgggatcatt ggcattatcg caggatttaa atgcatcaaa cattaccgaa
      721 aacatataac cgaaagtcaa acaggaaatt atgccgaatc acactaatgc ggttattctg
      781 acacaaccga ttcattcacc atctgatcaa ttatctcatc gtcaaaatca agctctctca
      841 aaatctctct ggtgtgctct ccaggttgtg aaggttgata cctcacccca aatcgattgc
      901 catccatttc tatcggcaaa gttggtagtt taacttgccc tccttcggga agatcgattg
      961 caaccaaacc atcagatgca ttcaaatgcg gatcttcgaa aaggtcttct ggcttggata
     1021 tcggtgcaaa cggtaatcct gtttcttcca atttctgcat caactccaat ttggtatatt
     1081 gtttgaatgt ttcagtgatg atggggatga tgagatctct atgctccact ctgccggcat
     1141 tttcattcaa atcagaattc tctaacaatt ccagtaaatc aaaagcctta caaaatgatt
     1201 gccactgtgc atcgctgaca accccaacaa acacttgctc atcccccttg gtttgaaaga
     1261 cctcataaat agcccaaacg gatttacgaa ccggcatcgg tggaaccgct tctccagtaa
     1321 tggcatattg agccatatgt tgtgtcatca aatacgccac attttcaaat aaagcactgg
     1381 ttattttttg gcctctacca gtagttttta gttgttcgca agcagctaaa ataccaatga
     1441 caccaaacat accacccata atgtcaatca ccgatgaacc agctctcaaa ggctggccaa
     1501 tgggtccggt catatgagca agcccaccca tcatttgagc aatttcatcc aaggctgttc
     1561 tattttcata agggccctgg agaaatcctt tggctgagca ataaatcaac ctgttgttta
     1621 aaggtttcag atcctcgtag ccgaatccaa gtctttccat ggtgccaggt ctgaaattct
     1681 cgatcagtac atctgattcc tgaatcagtt ttaatgcaat atccttgcct tggtctgatt
     1741 ttatatccag agcaatagat cttttatttc gattaaaaga agtaaaaaag ccaatcccag
     1801 aaccaggcaa taatctcgtc ttatctccag taaccggttc aattctgata acttcagcac
     1861 ccaaatcagc aagaatagca cctaccgcag gtcccataat catatgggtg aactctgtaa
     1921 cttttattcc tttaagcggt agattatctt gcgaaaacat tttcccagta tttgaatcaa
     1981 tcattcatta gattgcttat tctacctaca atagcagtat ttttcatcag agaccatgct
     2041 agcaaaacga gggataaact ggttaaacat agatctagat caaggattgt gatgaagatc
     2101 tgtgatacgt tgatttaaat taaaaataaa agatagaacc cttatgccca gttcaatttc
     2161 aaccatcgca ataggagtag agaacctcaa ttcagcactt gatctttggg ttgatacgtt
     2221 tgatctagaa ttggtaaaga aaataactgg tttagatgaa aacttagcaa cactatgggg
     2281 gatagatccc gataatataa gaactcaagc tttagtcaga acgcctggag tcgaaattgg
     2341 tcaacttcac ttagtggaat ttaagactcc caatgatccc gtcagaaaag atgccaatag
     2401 caccgatttg ggtcccaaga atctggac
//
LOCUS       DASCTH010000143         1610 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000196172, whole genome shotgun sequence.
ACCESSION   DASCTH010000143 DASCTH010000000
VERSION     DASCTH010000143.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000196172"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="QGH35_03050"
     CDS             <1..951
                     /locus_tag="QGH35_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006993824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL81544.1"
                     /translation="WASEVFNCNAPDTGNMEVLDRYGTEAQKQQWLEPLLKGEIRSGY
                     AMTEPAVASSDATNIETPIQLDGDEYVINGRKWWTSGAMNEACKLLILMGKTDPENSN
                     RHIQQSMILVPMDTPGVEVVRPLTVFGYLEAPFGHPEVSFTNVRVPKENIILGEGRGF
                     EIAQGRLGPGRIHHCMRLIGLAQRALELMCIRANNRVAFDQLLSAQGSVRESIAMSAS
                     EIEQARLLTLMTADQMDRYGNKETAALISMIKVVAPRMACQVLDRAIQIHGGAGVSGD
                     FFLAAAYAVARTLRLADGPDEVHLAFTAKKILKEHAPLKD"
     gene            955..>1610
                     /locus_tag="QGH35_03055"
     CDS             955..>1610
                     /locus_tag="QGH35_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011866658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HJL81545.1"
                     /translation="MNATAHSVDEARLSEYLEQHVPEFYGPVQSTKFKTGQSNPTFLL
                     EAASKKFVLRKKPPGKLLPSAHAVDREYKVLKALDNTDIPVPEVFHLCEDETIIGSIF
                     YLMEYVDGRIFWDPTLPEMSNQERQEIYEASNKVLASLHQIDIEKVGLSDFGKPGSYF
                     ERQTHRWIKQYRAAETTHYDEVESLIQWLGENMPKDDGMVSIVHGDYRLYNMMFDHKQ
                     T"
BASE COUNT          498 a          328 c          379 g          405 t
ORIGIN      
        1 tgggcatctg aagtttttaa ctgcaatgca cccgataccg gcaacatgga agtgctggat
       61 cgttatggca cagaggcaca aaaacagcaa tggttggagc ccttgttgaa aggagaaatt
      121 cgctcaggct atgccatgac agaacccgct gttgcttcat cggatgcaac caacatcgaa
      181 acaccgattc agctcgatgg agatgaatat gtcatcaatg gaagaaaatg gtggacctca
      241 ggagcaatga atgaggcctg taagttattg atattaatgg gtaagacaga tcctgaaaat
      301 agcaatcgtc acatccaaca atcaatgatt ttagttccca tggacacccc tggggttgaa
      361 gtcgtcaggc cgttaacggt ttttggttac ttggaggcac cctttggaca tcctgaagtc
      421 agttttacca atgtcagggt accgaaagaa aatattattc ttggagaagg aagaggtttt
      481 gaaattgcac aagggcgatt gggtcctggt cgcatccacc attgtatgcg tttaattggt
      541 ctggcccaac gtgcattaga attaatgtgt attcgagcaa acaacagagt tgcttttgac
      601 caactattaa gtgctcaggg ctctgttagg gaaagcatcg cgatgtctgc cagtgaaata
      661 gaacaagcgc gccttttaac attaatgact gcggaccaaa tggatcgtta tggcaataaa
      721 gaaactgctg ctctgatcag tatgattaag gtagttgcac caagaatggc ttgtcaagtc
      781 cttgatcgag ccattcaaat acatggtggg gctggtgtat caggagattt cttcctggct
      841 gcagcttatg cggtcgcacg cacgctcaga ctagcagatg ggccggatga agtacattta
      901 gccttcacag caaagaaaat actgaaggaa cacgcacccc ttaaagatta gtaaatgaat
      961 gccactgctc acagtgtcga tgaagctaga ctgagcgaat accttgaaca gcacgttcct
     1021 gaattctatg gaccggtcca atcaacaaaa ttcaaaaccg gacaatccaa ccccacattt
     1081 ttattggaag cggcaagcaa gaagtttgtc ttgcgtaaaa aaccacctgg caagctgttg
     1141 ccgtccgccc atgcggtaga tagagaatac aaggtcttaa aagcactgga taataccgat
     1201 attccagttc cagaggtttt tcacctatgt gaagatgaaa ccattattgg tagcattttt
     1261 tatctcatgg agtatgtcga tggcagaatt ttctgggatc caacgctccc tgaaatgagc
     1321 aaccaagaac gacaagaaat ctatgaagca agcaacaaag tactagcttc attgcatcag
     1381 attgatattg agaaagttgg tttatctgac tttgggaaac caggaagtta ttttgaacgc
     1441 cagacacaca gatggatcaa acaatacagg gcagcagaaa caactcacta tgatgaagta
     1501 gaatcactga ttcaatggtt aggggaaaat atgcccaagg atgatgggat ggtgtctatt
     1561 gtccatggtg attatcgtct ctacaacatg atgtttgacc acaaacaaac
//
LOCUS       DASCTH010000144         3434 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000199827, whole genome shotgun sequence.
ACCESSION   DASCTH010000144 DASCTH010000000
VERSION     DASCTH010000144.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000199827"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="QGH35_03060"
     CDS             complement(<1..275)
                     /locus_tag="QGH35_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HJL81546.1"
                     /translation="MYINFWYPVLLSKELKDAPIRVRMLGQNFVAFRDSKGKANTLSD
                     ICPHRGGALSGGIIKENCIQCPYHGWRFDGEGHCHRIPSLGKDAKIPV"
     gene            467..1072
                     /locus_tag="QGH35_03065"
     CDS             467..1072
                     /locus_tag="QGH35_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81547.1"
                     /translation="MEENRKPNNTDNKKDGPPVSAMKPENLAPPTGMEGREADLATAM
                     QVLVKTIKMQMPYPHDTNDALLKAHLNTIQFAKNKGLLEELVAHDLETMKPLLERSKA
                     MIEKTGNKELALIMNFERTGCHFQMILETKNEPGKRTFIFPFKKVLNAAKRLGQFDLT
                     EEEILESWWKPRYLNYAQAMGVEFKISDLDKNGTVTVALVD"
     gene            complement(1099..1770)
                     /locus_tag="QGH35_03070"
     CDS             complement(1099..1770)
                     /locus_tag="QGH35_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HJL81548.1"
                     /translation="MIPTHIQKAVSSKNRPASDQERDSLRKPGEVLRFFDVKEGDKIG
                     ELNAGRGYFSSIAAHAVGENGMVIAHTSPMSVKRWKGNPIEKRLEEFPQSNLIPVVGD
                     MESPNFPGHLDKIFNIMTYHDAVWTGVDRDRMNTSIFNTLRDAGIYGILDHHARPGQG
                     KDDCQEIHRIEKEFVIDEILRAGFILDKESNLFENPEDDLTSMVFEKGIRDQTSRFLL
                     CFKKS"
     gene            1875..3155
                     /locus_tag="QGH35_03075"
     CDS             1875..3155
                     /locus_tag="QGH35_03075"
                     /inference="COORDINATES: protein motif:HMM:NF018004.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HJL81549.1"
                     /translation="MRKIKQMTEIKKNNLVKAFKALRPIPEKVLEVIAPPYDVINSDE
                     ARILAAHKPLSFLHISRPEIDLPVGTSYHNPEVYQQGLKNFKNLIDKEILIEEDIDSI
                     YVYEISLGTHQQTGIGCIASLDAYEHNLIKKHEHTTPKKEQDRCNNITTLNAQTGPVL
                     LAHKKNSDLKDMLSEITIHKLPLYDVEANDGSRHKIWRLSNTDTLQSILALLNDMDAL
                     FIADGHHRSAAAALVRKNKKAANNNHNGTENYNYVLVVAFPEDEMNILDYNRVVKSAN
                     GHSKKSLLQAADQFFDIETMASTFKSSQPNQFGMYFQGTWYKLELKPTHKKAIDPVKS
                     LDVSLLHDYLLEPILGIQDERIDQRIDFVGGIRGIEALQERVDSGEMAIGFSLYPTPM
                     NALISVAEANQIMPPKSTWFEPKLLDGLLTHLIG"
     gene            3248..>3434
                     /locus_tag="QGH35_03080"
     CDS             3248..>3434
                     /locus_tag="QGH35_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81550.1"
                     /translation="MDAETIKEKANSADENITFTDDACEALTQVPDFAMDMAINHMVN
                     AAKDQGVDTIDPEFLNAN"
BASE COUNT         1170 a          688 c          586 g          990 t
ORIGIN      
        1 actggaattt ttgcatcttt acccaatgat ggaattcggt ggcaatgtcc ttcaccatca
       61 aatcgccaac cgtggtatgg acattgaatg caattctctt tgatgatgcc cccagacagt
      121 gctccacctc gatgaggaca gatatcactt agagtgttcg ctttaccttt cgaatccctg
      181 aatgctacaa agttttgtcc cagcattctt acacgaatgg gagcatcttt taattccttg
      241 ctcaggagta ctggatacca aaaatttata tacattctaa ttgtttatca tcgttataca
      301 ttcatattag aagaattgac tattgcaata atattaataa aaatctttac actgggagat
      361 caaatattat atcgaattta tgattttacg tcctgtctga ttcatcagac ataggctata
      421 attgcataga tttttttaac attgaaagat acttaggatt tgaattatgg aagaaaatag
      481 aaaaccaaat aacacagata acaaaaaaga tggacctcct gtgagtgcta tgaaaccaga
      541 aaatttagct ccaccaacag gaatggaagg aagggaggca gatttagcta cagcgatgca
      601 agtactggta aaaacaataa aaatgcaaat gccctaccct catgatacaa atgatgcctt
      661 gctgaaggca cacctaaata ctattcagtt tgcaaaaaat aaaggacttc tagaagagct
      721 ggtagcacac gatcttgaaa ccatgaaacc tttgcttgaa cgatccaaag ctatgataga
      781 aaaaaccggc aataaagaac tggcattgat tatgaatttc gaacgcacag gttgtcattt
      841 ccagatgata ttggaaacaa agaatgaacc ggggaaacgg acatttattt ttccttttaa
      901 aaaagtattg aatgcagcta aaagattggg tcaattcgat ctcactgaag aagagattct
      961 agaaagctgg tggaagcccc gttatcttaa ttatgctcag gctatgggag tggaatttaa
     1021 aatctctgat ctggataaaa acggcactgt tacagttgca ttggtagatt gaatcaataa
     1081 tctgttggtt gactagcact aagacttctt gaaacacaac aggaatcgac tggtttgatc
     1141 cctgatcccc ttttcaaaaa ccattgaagt taaatcatct tctgggttct caaaaagatt
     1201 ggattctttg tccaaaataa acccagccct cagaatctca tcaataacaa attctttttc
     1261 aatgcgatga atctcttgac aatcatcttt accttgaccg ggtctggcat gatgatccaa
     1321 tattccataa atgccagcat ctcttaaggt attaaaaata cttgtattca tgcgatccct
     1381 atcgacccct gtccagactg catcatgata ggtcatgata ttaaatatct tatccaagtg
     1441 acctgggaaa ttaggcgact ccatgtctcc aacaactgga attagattgg attgtggaaa
     1501 ttcttcgagt cttttttcaa ttggattgcc tttccagcgt ttaacactca ttggcgacgt
     1561 atgagcaata accattccat tttctcccac tgcatgtgct gcaattgatg aaaaataacc
     1621 tcttccagca ttgagttctc caatcttgtc tccttccttt acatcaaaga accttagaac
     1681 ctcgcctggt tttcttaatg aatctctttc ctgatcactt gcaggtctat tcttgctgct
     1741 gactgccttt tgaatatggg tcggtatcac aaaaattact ctccatgatt ttttaaaagt
     1801 gacttacaat tatcgaatat aatccacctt tatacataat aattttaacc tatagtaatg
     1861 agtactaatt ttttttgaga aaaattaaac aaatgactga gataaaaaaa aataatctag
     1921 ttaaagcatt taaagcacta cgaccaatac ctgaaaaagt attagaagtc attgctccac
     1981 cctatgatgt tataaactca gatgaagcta ggatattagc tgcacataaa ccactgagct
     2041 tcctccatat ttcaaggcct gaaattgatt tacccgttgg tacttcatat cacaatccgg
     2101 aagtctacca acagggttta aaaaatttca aaaaccttat cgataaagaa attctaatag
     2161 aagaagacat agattcgatc tacgtttatg aaatttcgct tggcacccat caacagaccg
     2221 gtattggttg tatcgcatca ttggatgcct acgaacataa cctaatcaaa aaacatgagc
     2281 acaccacacc aaaaaaagaa caggatagat gcaataatat taccaccctt aatgctcaaa
     2341 caggtcctgt attgctagca cacaaaaaga acagtgactt aaaagacatg ctctcagaaa
     2401 tcactattca taaactcccc ctttatgacg tagaggcaaa tgatggatct aggcataaga
     2461 tttggcggtt atcaaacacc gatacacttc aatcaattct agccctgtta aacgatatgg
     2521 acgccttatt tatagccgat ggtcatcatc gttcagcagc tgctgcactg gttagaaaaa
     2581 acaagaaagc agcaaataac aaccacaatg gcacagaaaa ctataattat gttctcgtgg
     2641 tcgcatttcc tgaagatgaa atgaacattc tagactacaa tcgtgtcgtt aaaagtgcca
     2701 acggtcattc aaaaaaatct ttattacaag cggctgatca attttttgat attgaaacaa
     2761 tggcttcgac ctttaaatct agccagccca atcaattcgg tatgtatttt caagggactt
     2821 ggtataaatt ggaactcaag ccaacacata aaaaagcaat agatccagta aaatcactag
     2881 atgtttccct tctgcatgat tacctcctag aaccaatatt aggaatccag gacgaaagaa
     2941 ttgatcaaag aattgatttt gtaggtggga tcagggggat tgaagcactc caagaaagag
     3001 tggattctgg agaaatggca attggtttct ctttgtatcc aactccaatg aacgctctca
     3061 tctctgttgc tgaagcaaat caaatcatgc caccaaaatc tacctggttt gagccaaaat
     3121 tactagatgg tttgctcaca cacctcattg gttaaactca atcacctcaa caattattct
     3181 tcacgtttga atcaaaattt gacacaatta accactaata cttactttat ctaaaaaagg
     3241 aaaaatcatg gatgcagaaa caattaaaga aaaagcaaat agtgcggatg aaaacattac
     3301 atttactgac gatgcttgcg aagcattgac acaggtccca gattttgcaa tggatatggc
     3361 cattaaccat atggtcaatg ctgccaaaga ccaaggtgtt gacacaatcg atcctgaatt
     3421 cttgaatgcc aata
//
LOCUS       DASCTH010000145         3894 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000202742, whole genome shotgun sequence.
ACCESSION   DASCTH010000145 DASCTH010000000
VERSION     DASCTH010000145.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000202742"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="QGH35_03085"
     CDS             complement(<1..546)
                     /locus_tag="QGH35_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJL81551.1"
                     /translation="MNDSTNSLSLEGLVFEAIEVNIDGHILSITLNRPEKRNAINTVM
                     GNEITYALSYASQESNVRVVIIQAKGDVFCAGGDLGGMLGKEQQTTSTVPKLGDTSNV
                     SLLIRNLCKPLICKIQGPAYAGALLMICNATHAIAADHVEFATPEIKRGIWPFMVMAG
                     LFRVMPKRQGLDFIMRGETIDA"
     gene            complement(547..2319)
                     /locus_tag="QGH35_03090"
     CDS             complement(547..2319)
                     /locus_tag="QGH35_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006370727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HJL81552.1"
                     /translation="MAKDSIRIANCSGYYGDKLSAAKEMVEGGPIDVLTGDYLAELTM
                     AILYSQKLQRGEDKGYVGTFLKQLKDIAKTCKDQKIKIVSNAGGLNPKSMAKEVETIL
                     TELDVDATVAYIDGDDLVEELEQLQAAGELLDNIDRKIPLAQSDCKVLSANTYLGGWG
                     IKEALDLGADIVICPRVTDASVTMGPAAWKFNWQRDDYDALAGSLAAGHIIECGAQAT
                     GGNYSFFEEVSTFKDIGYPIAEIEEDGAFTITKHPGTGGLVSIGTVTAQLLYEIAAPE
                     YLNPDVIAHFDTLKMQQVGDDRVRVTGCKGSSPPSTHKVCINLAGGYRNGIELIITGL
                     DIEQKVKIFTEALFDLLGGKEQFDDVSIELHRQEKENPQSNEEAMASLRINVTSKDPN
                     KVGRMFTAKYIELALANYPGFFGKSPPGSGAPFIRYWPALIDSKHIKESVHIGEQTVK
                     ITPTNQLGLDEHTYQKEAQKILPLVSEHHQQIHFGRIYGTRSGDKGGCANLGVWAKTD
                     RAFEFLYHFLTVDRLKQLLPDIANFPIDRYELTNIKSLNFYIHEILQDGVSSSTRIDA
                     QAKSLGEYLGAKLIDVPVEIIEEL"
     gene            complement(2329..2943)
                     /locus_tag="QGH35_03095"
     CDS             complement(2329..2943)
                     /locus_tag="QGH35_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HJL81553.1"
                     /translation="MTIASSRKLKSPIKLAAKHIKVIEDFEELLEQGISGLTMADIAA
                     KLKVSLRTLYEIAPSKEDLITSQMHRLLTNVGQEASASIQEIQSPLSKLKKYIEIGYK
                     AVGPRLRKFHKDLNRIKGVEKMIDYHRDYFISQIEELLDEAIKHKEIEDTDTHVVAIT
                     LGVVPVLISDYFFQEDSFQQELENTPEDSAHLVADLILASLERK"
     gene            complement(2955..>3894)
                     /locus_tag="QGH35_03100"
     CDS             complement(2955..>3894)
                     /locus_tag="QGH35_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014804.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HJL81554.1"
                     /translation="IYAEDPENNFLPSTGKLIANQIKPKAGIRWDSGVEAGMLIGTDF
                     DPLLAKVIAHGSTRLDAARKLASELENTHFGGFVTNVEFLANVLRHKEFIQGNTTTGF
                     IDEFSPSRSLVLEDDQQRFVAITAALWLQGLNRSQAVVLENMPSGWNNARLPDQRIVF
                     EMDDRLLEIHYKRNRDGSFTVDNLQQAIVHHWCESEVDIEIDGVRTLSSVTMEGDQLL
                     VQCLGGNKLLNIQPRFVSSQEQEQQGSLVSPMPGKVVELHVKKGDRVKAGDNLLMIEA
                     MKMNHNVKANEDGIIAELFVQENDQLDYGAVLMIIE"
BASE COUNT         1028 a          891 c          732 g         1243 t
ORIGIN      
        1 tgcatcgatt gtctctcctc tcataataaa atccaaacct tgtctcttgg gcatcactct
       61 gaacaatcct gccattacca taaacggcca gatgcctcgt ttgatttctg gggttgcaaa
      121 ctctacatga tctgctgcaa ttgcgtgggt tgcgttacag atcatcaaca gtgccccagc
      181 ataagctggt ccttggatct tgcagattaa tggtttacac aagttacgta ttaataaact
      241 gacgtttgat gtatccccaa gtttaggtac ggtggaagtt gtttgttgtt ctttgcctaa
      301 cattccaccc aaatctcccc cagcacaaaa aacatctcct ttggcttgaa tgatgactac
      361 tctgacattg ctttcctgcg atgcatagga aagagcatag gttatctcat tccccataac
      421 cgtgttgatc gcattgcgtt tttctggtcg atttagggta atcgacagta tgtgtccatc
      481 gatgtttact tcaattgcct caaaaaccaa tccttcaaga gatagcgaat ttgttgaatc
      541 attcatttac agttcctcaa taatttctac tggtacatca atcaatttgg cccctaaata
      601 ttcaccaaga gattttgctt gtgcatctat cctggtactg gaagagactc cgtcttgcaa
      661 gatctcgtgg atgtagaagt ttaaggactt aatattggta agctcgtatc tatcaatcgg
      721 aaagttggct atgtcaggga gtagttgttt cagtcggtca acagtcagaa aatgataaag
      781 aaattcaaag gctctgtcag ttttagccca aacaccaaga ttagcacaac cgcctttatc
      841 accagatcgt gtaccgtaga ttcttccaaa atggatttgt tggtgatgct cactcaccaa
      901 tgggagaatc ttttgtgctt ctttctgata tgtatgttca tccagtccca gttgattggt
      961 cggggtaatt ttcactgttt gttcgccaat atgaacgctt tccttaatat gttttgaatc
     1021 gattaaagcc ggccaataac gaatgaacgg ggctcctgaa ccgggtggtg atttaccaaa
     1081 gaatcctgga taatttgcaa gcgccaattc aatatatttg gcagtgaaca ttctacccac
     1141 tttgtttgga tcttttgatg tgacgttgat ccgtaaggat gccatcgcct cctcgttgct
     1201 ttgcgggttt tctttttcct gtctgtgaag ctcaatagaa acatcatcaa attgctcttt
     1261 accgcctaat aaatcaaata gtgcctcggt aaatattttt actttttgtt caatatccaa
     1321 gccggttatg atcaactcta ttccatttcg ataaccccct gcaaggttaa tgcagacctt
     1381 atgagtagac ggcggactac tacccttgca tcccgtaaca cggaccctgt cgtcacctac
     1441 ctgttgcatt tttaaggtat caaaatgggc aattacatct ggattcagat actccggtgc
     1501 agcaatctca tagagtagtt gggccgttac ggtacctatg gagacaaggc cgcctgtacc
     1561 ggggtgtttg gtaatggtaa aagcgccatc ctcctctatt tcagcaatgg gatatcctat
     1621 atctttaaaa gtagacactt cctcaaagaa cgagtaattt ccaccggttg cctgagcacc
     1681 gcactcgatg atatgaccag ctgccaatga tccggcaagg gcatcgtaat catctctttg
     1741 ccaattaaat ttccaagctg caggtcccat ggttacagat gcatcagtaa ctctggggca
     1801 aatcactatg tctgcgccca aatccaatgc ctctttaatt ccccaaccac caagataagt
     1861 atttgcactc agaactttac agtcggattg tgccaaaggt atttttctat caatattatc
     1921 caatagctca cctgcagctt ggagttgttc cagttcttca actaaatcat ccccatcaat
     1981 ataggcaaca gttgcatcga catccaattc ggtaaggatg gtttcaactt cctttgccat
     2041 tgattttgga ttgagacccc ctgcattgga aacaatttta atcttctggt ctttgcacgt
     2101 ttttgctatg tctttaagtt gtttcaaaaa cgtccccaca tatcccttgt cttcacctct
     2161 ttgtaatttt tgtgaataca gaatagccat ggttagttca gcgaggtaat ctccggttag
     2221 gacatcaatg ggacctccct caaccatctc tttagcagca gagagtttgt caccataata
     2281 accactgcaa ttagcaattc ttatgctgtc tttagccata aattttcctc attttcgttc
     2341 caggcttgcc aagattagat cggccactaa atgtgctgag tcctcaggtg tgttctctag
     2401 ttcttgttgg aagctgtcct cttgaaaaaa ataatcagaa atcaaaacag gcacaacacc
     2461 cagtgtaata gccaccacat gggtgtctgt atcctctatt tccttatgct taatcgcttc
     2521 atcgagtaat tcttcaattt gactaataaa gtaatctcga tggtaatcaa tcattttctc
     2581 aacgcctttg attctattga gatctttatg aaatttccta agtcttggtc caacagcttt
     2641 atatccaatc tcaatgtatt tttttaattt agacagtggt gactggattt cctgtatcga
     2701 tgcactggcc tcttgaccta cattggtaag caagcgatgc atctgtgatg taatcaaatc
     2761 ttctttgctc ggtgctattt cgtacaacgt cctcagtgaa actttcaatt tagcagcaat
     2821 atctgccatg gttaaacctg aaattccttg ttcgaggagt tcttcaaaat cctcaattac
     2881 ttttatatgc ttggcagcta acttgattgg ggattttagt tttcttgaag atgcaatggt
     2941 catgattatc ttttttactc aattatcatt aaaacagccc catagtccaa ctggtcattt
     3001 tcttgaacga aaagctctgc aatgatcccg tcttcattgg ctttgacatt gtggttcatt
     3061 ttcatcgcct cgatcatgag taaattatca ccagctttaa ctcggtctcc tttttttaca
     3121 tgaagctcaa caacttttcc tggcattggt gaaacaagag agccctgctg ttcctgttct
     3181 tgggaagaga caaatctggg ttggatgtta agtagtttat tgccacctag gcattgaacc
     3241 agcagctgat caccctccat agtgacactt gatagagttc taacgccgtc aatttctata
     3301 tccacctctg actcacacca atgatgcact attgcctgct gcaagttgtc cacagtaaaa
     3361 gatccatcac gatttctttt gtagtggatt tcaagcaatc ggtcatccat ctcaaataca
     3421 attctctgat caggaagcct cgcattgttc cacccagaag gcatgttctc caacacaact
     3481 gcctgtgacc tatttaatcc ttggagccag agtgcggcgg taatggcaac aaatctttgt
     3541 tgatcatctt caagaaccag agatctggaa ggactaaatt catcaatgaa acccgttgtg
     3601 gtattgcctt ggataaattc tttatgtcgc agtacattgg ccaagaattc cacattggta
     3661 acaaatccac caaagtgagt attctcaagt tcactagcaa gctttctggc agcatccagt
     3721 cgggttgaac catgtgctat tactttggcc aatagggggt caaaatctgt tccaatcaac
     3781 atccctgcct ccactcctga atcccaccga atccctgctt ttggtttaat ttggttggca
     3841 atgagcttac cagtggatgg aaggaaatta ttttcagggt cttcagcata aatc
//
LOCUS       DASCTH010000146         2022 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000203529, whole genome shotgun sequence.
ACCESSION   DASCTH010000146 DASCTH010000000
VERSION     DASCTH010000146.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000203529"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(142..975)
                     /gene="fghA"
                     /locus_tag="QGH35_03105"
     CDS             complement(142..975)
                     /gene="fghA"
                     /locus_tag="QGH35_03105"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014819926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HJL81555.1"
                     /translation="MKLSLEAENKTFGGHYQVHSHWGESTNCNMVFSIFLPPQSEVKK
                     LPVLIWLSGLTCTEENFRIKAGVQRYATEAGIIVVSPDTSPRGEGVPDDPDYGFGQGA
                     SFYLDATQEPWSEHFNMYSYILKDLLSILESNFPTDMKRVGIFGHSMGGHGALTIAFK
                     NPELFKSVSAFSPICAPTKAPWGENAFTRYLGEDTSHWQDYDACDLVRTKGWSSDILV
                     DQGDDDEYLTDYLKPDLLEQACEEKGISIKLRRHAGYDHSYFFVSTFIGDHILWHSER
                     L"
     gene            complement(976..>2022)
                     /locus_tag="QGH35_03110"
     CDS             complement(976..>2022)
                     /locus_tag="QGH35_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015515.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HJL81556.1"
                     /translation="MIDASIGQINEINESAYYIYLNSILYSFYPVFMLAFVLTIAGSG
                     RDFAAMHKYEIAARAGEDYSLQGAEMGYTNESESKQLEPKEPMRAKAYNAIIPILIFI
                     GSVIVGLFMTGEGNSVMEIIGSADAYQALMWGSLIAVMFAVLLTLVQGIMSLEETVQS
                     WYGGIKFTIFAIIILIMAWSLGETTEILQTAKYLVSILGAFLPVAFIPTIVFILSAAT
                     AFATGTSWGTMGIFYPIVVPLVWQVLLMNGLADPDHMFILYSSVACVLSGAVWGDHCS
                     PISDTTILSSMASGCSHIDHVTTQLPYAITVASIALLIGTIPTGFGISPWIMILIGLS
                     TVVATVFILGKKID"
BASE COUNT          659 a          456 c          387 g          520 t
ORIGIN      
        1 ttgttcacgc aatggctatt ccacataata ttgatgaatt cagggaactg tcaaaaattg
       61 taaatcttcc cgaaagaact atctctcaaa atccaaaacg gagaagacaa acaacccagt
      121 cgtaaatcag acaatccccc ctcacaagcg ttctgaatgc cacaaaatgt gatcacctat
      181 aaaagttgaa acaaaaaagt aactgtgatc atagcctgca tgtcttctta gcttgatcga
      241 aattcctttc tcctcgcaag cttgctccag aagatctggt ttcaagtagt cagtcagata
      301 ttcatcatca tcgccctgat caaccaaaat atcgcttgac caaccttttg tacgaactaa
      361 atcacaggca tcataatctt gccaatgact ggtatcctcg cctaaatatc gagtgaaggc
      421 attttcaccc caaggcgctt ttgtgggagc acaaattggg gaaaaagctg aaacagattt
      481 aaatagctca gggtttttaa aggcaatggt taaggcacca tgaccgccca ttgagtggcc
      541 aaaaatacct actcttttca tatcggttgg aaaattagac tctagaatgg aaaggagatc
      601 tttaaggatg tagctataca tattgaaatg ctcagaccaa ggttcttggg tggcatccag
      661 ataaaaactc gcaccctggc cgaagccata atcgggatca tctggcaccc cctcacccct
      721 tggactagta tcaggagaca ccacaatgat acctgcctca gtagcgtaac gctgaactcc
      781 agctttgata cgaaagtttt cctccgtaca cgtcagtcca gataaccaaa tcaggaccgg
      841 taatttctta acttctgact gtggaggcaa aaaaattgag aacaccatgt tacagttggt
      901 ggattcaccc cagtgagaat gcacttgata gtgaccacca aaagtcttgt tttctgcttc
      961 aagtgatagc ttcatctaat caatcttctt acccaagatg aaaactgttg cgaccacagt
     1021 tgaaagacct attagaatca tgatccaagg tgagatgccg aatccggttg gaattgtgcc
     1081 aatcaataaa gcaattgagg caacggttat agcataaggt aattgggttg taacgtgatc
     1141 tatgtgacta caacctgaag ccatcgatga caagatagtg gtatctgaaa taggagaaca
     1201 atgatcaccc caaacagctc cactgagaac acatgcaact gatgaatata gaataaacat
     1261 atgatcagga tctgccagcc cattcattaa taagacttgc caaaccaaag gcaccacaat
     1321 gggataaaat attcccatag tcccccaact ggtccctgta gcaaaggctg tggctgccga
     1381 gagtataaat actatagttg gtataaatgc tacaggtaaa aatgcgccaa gaatggagac
     1441 caaatacttt gcagtctgca gtatctcagt tgtctcacca agagaccaag ccatgatcag
     1501 aataatgatt gcaaagatcg tgaatttaat tcccccgtac caggactgta cggtctcttc
     1561 tagggacata ataccctgga caagggttaa taagactgca aacatcactg cgatcaagga
     1621 tccccacata agagcttgat aggcatcggc agaaccaata atctccatga cagagttgcc
     1681 ttccccggtc ataaataatc caacaataac acttccgatg aatataagaa ttgggataat
     1741 cgcattataa gcttttgccc tcattggctc ttttggctca agttgcttgg attcagattc
     1801 attggtatag cccatctcag ctccttggag ggaataatct tcccctgccc ttgctgctat
     1861 ttcatatttg tgcattgctg caaaatctct accagagccc gcaatggtta atacaaaggc
     1921 caacataaaa acgggataaa aactatatag aattgaattt aaataaatgt aataagcaga
     1981 ttcattgatt tcattaatct gcccgatgga tgcatcaatc at
//
LOCUS       DASCTH010000147         3190 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000206685, whole genome shotgun sequence.
ACCESSION   DASCTH010000147 DASCTH010000000
VERSION     DASCTH010000147.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000206685"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="QGH35_03115"
     CDS             <1..393
                     /locus_tag="QGH35_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJL81557.1"
                     /translation="KQAYFNTFGGNPVACAAALAVINFIEKNKLVEHAESVGVYFRQA
                     LSSLSKAHKIIHQIRGGGLFIGVELVSQHSAQDTSAVINTLKELGVLAGITGPENNVI
                     KFRPPMTFQKEHADMVVSKLNQALDSLS"
     gene            404..1579
                     /locus_tag="QGH35_03120"
     CDS             404..1579
                     /locus_tag="QGH35_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003291289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HJL81558.1"
                     /translation="MANLTSLKVLDFSTLLPGPYATMMLADMGADVIWVEPIKTKAAL
                     IEATAMRSYLGRSKRSISINLKADKAHQIIESLIQEYDVIVEQFRPGVMERLGLDYAA
                     LSEIQPNIIYCSITGYGQTGPLKNRAGHDINYLSIAGVSSFSGTKEKGPVLSGLQIAD
                     IAGGSMHAVTGILAALVYRQETGKGQWIDVSMTDASFAMNSIHAADYLVNQNNPSYET
                     NALNGGSFYNYYPTKDGKYFSVGSLEPKFFQQLSEALGNPEMFDAKYFHPENQEQIIL
                     FIQEAFQQKTYAEWVDIFSNLDACVEPVIGLDEAVKHEQIQARKMVVDVSSLGGEEEK
                     QIGSPIKFSHSTPQHSHTGPPVGLNTEEILLEAGFNPAAIQGFINDGVLFQETDKKP"
     gene            complement(1592..2785)
                     /locus_tag="QGH35_03125"
     CDS             complement(1592..2785)
                     /locus_tag="QGH35_03125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HJL81559.1"
                     /translation="MAEAVIVDSVRTGLAKSFRGGFNQTRADDMTAHIINALMDRNPK
                     VAPELVEDVILGCGYPEGPQGNNIARTSAVLSKLPIKTGGTTVNRFCSSGLQTIAIAG
                     AQVQSGFADCIIAGGVESISTTQGHINTYMFENEKITKAVPGIYHAMGTTAETVAKRY
                     NITREAQDEYALSSQLKCAEAQNQGLFDDEIIPMNTVMKVVNKETGEESETETVVDKD
                     NCNRPNTTIEGLAGLNPVFDPEGGSVTAGNSSQLSDGASVTMVMSEKKANELGLEPLA
                     YFRGFTVTGCEPDEMGIGPVFAVPKLLKTAGLEVADIGLWELNEAFASQVVYIRDQLN
                     LPTEKLNIHGGSIAIGHPFGMTGSRQVGHLTRELKRRKIRHGVVTMCVGGGMGAAGLF
                     ESISH"
     gene            complement(2899..>3190)
                     /locus_tag="QGH35_03130"
     CDS             complement(2899..>3190)
                     /locus_tag="QGH35_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HJL81560.1"
                     /translation="WKDKVLCSDQERTCLRSLLSLGLSDSFRLFEQLEEQYSWWDYRA
                     AAYRRKMGLRIDLILLSAPLVKHCVSSTIDETPRGWEKPSDHTPVLVEIDTG"
BASE COUNT          933 a          697 c          636 g          924 t
ORIGIN      
        1 aaacaggctt attttaatac ttttggcggt aaccctgttg cctgtgctgc tgctttagcg
       61 gttatcaatt tcattgaaaa aaacaagctg gtggagcacg ccgagagtgt aggtgtttat
      121 ttcagacaag ccctaagtag cctttcaaaa gctcacaaaa taatccatca aattagaggc
      181 ggaggcttgt ttataggagt agagcttgtt tcacaacact cagcacaaga cacatcagct
      241 gtaattaaca cattaaaaga gctaggagtc ctagcgggca tcactggacc cgaaaacaac
      301 gttataaaat tcagaccacc tatgaccttt caaaaagaac atgccgacat ggttgtgtcc
      361 aaactgaacc aggcattaga ctcactgtcc taggagaaaa actgtggcaa atctaacatc
      421 actcaaagtg cttgatttct caacgttgct tcccgggcct tatgcaacta tgatgttggc
      481 cgatatgggt gcggatgtga tctgggttga gcccatcaag accaaagctg ctttaataga
      541 agcaacagct atgagatcgt atttaggaag atcaaaacgc tctatttcta tcaacctgaa
      601 ggcagataag gcccaccaaa ttatagaaag cttgattcaa gaatatgatg ttattgttga
      661 acaattcagg ccaggtgtca tggagcgtct aggtttagac tatgcggcac tttcagagat
      721 acaaccaaat atcatatatt gttccatcac ggggtatgga caaactggac cattgaaaaa
      781 tcgtgcgggt cacgatatta actatttatc aatagcaggt gttagtagtt ttagtggcac
      841 caaagaaaaa ggaccagtgt tgtcgggtct ccaaattgct gacattgcag gtgggtccat
      901 gcatgcagta accggtatac ttgccgcatt agtttataga caagaaacag gaaaaggtca
      961 gtggatagat gtcagtatga cagacgcaag ctttgccatg aattcgatcc atgctgccga
     1021 ttatttagtt aatcaaaata atccaagcta tgaaaccaat gctcttaatg gaggcagttt
     1081 ttataattat tatccaacta aagatggcaa gtatttttct gttggcagtc tggaaccaaa
     1141 attttttcaa cagctttctg aggcactagg caatccagaa atgtttgatg caaaatactt
     1201 tcatcctgag aaccaagaac aaataattct atttatacaa gaagcatttc agcaaaaaac
     1261 atatgctgag tgggttgata ttttttctaa cttggatgcc tgtgtcgagc ctgtaattgg
     1321 tttagatgaa gctgtcaagc atgaacaaat tcaagccaga aaaatggttg ttgacgtttc
     1381 ttctttggga ggagaagaag agaagcaaat aggtagccct attaaatttt cccattccac
     1441 gcctcaacac tcacacactg gaccgccggt cgggttgaat actgaagaaa ttctacttga
     1501 ggctggtttc aaccctgcag ctattcaggg ttttattaat gatggagttc tctttcagga
     1561 gacagataaa aagccataaa atctcttttg actagtgtga gattgattca aacagacccg
     1621 ctgctcccat accaccgccc acacacattg ttacgacccc atgcctgatt tttctacgtt
     1681 tgagctcacg ggttaaatga cccacttgtc tggatccagt catgccaaaa ggatgaccaa
     1741 ttgctattga accgccatga atatttaact tctcggttgg caggttaagt tggtctcgta
     1801 tatagaccac ttgtgaggca aaggcttcgt ttagttccca aagacctata tcagcgactt
     1861 ctaaaccagc tgtttttaaa agtttcggca cagcaaaaac gggaccaata cccatctcat
     1921 caggctcaca acccgtgact gtgaaccctc taaaataagc taaaggctca agaccgagtt
     1981 catttgcttt tttctcactc ataaccatcg ttaccgaagc tccatctgag agttgtgaag
     2041 agtttcctgc agtaacggag cccccctcag ggtcaaaaac aggattgagt cctgcaagac
     2101 cttctattgt tgtattgggc cgattgcaat tatctttatc aacgaccgtt tctgtttcag
     2161 attcttcacc ggtctctttg ttaacaacct tcatcactgt attcatagga ataatctcat
     2221 catcaaacaa accctgattc tgagcttctg cacatttcaa ctgactggat agagcatact
     2281 catcttgagc ttcccgggtt atattatacc tcttagcaac agtctcagca gtggtgccca
     2341 tggcatgata aattccaggc actgcttttg ttattttttc attttcaaac atgtaagtat
     2401 tgatgtgtcc ttgagtagtg ctaatggact ccaccccccc agctatgatg caatcagcaa
     2461 aaccactctg aacttgggct ccagcgatag caatggtttg tagtcctgat gagcaaaatc
     2521 tattgacagt agtgccccca gttttgatcg ggagctttga taagactgca gaggttctgg
     2581 caatattatt tccttgtggt ccctcgggat atccacaacc tagaataacg tcttcaacaa
     2641 gttctggagc cacttttgga tttctgtcca ttaaagcatt aatgatatga gcggtcatat
     2701 catcggctct ggtttggtta aaaccgcctc taaaagattt tgctaaccca gttcttacgc
     2761 tatccactat tactgcttct gccatgattt accctccgtt ttattcttaa gcaatattac
     2821 tattaagctt ctaaatttta cacgaaaatc agattcttta taatgaagcg aaacgctcct
     2881 gggtcagtaa tctagatatc aaccagtgtc aatttcaacc agcactggtg tgtggtcaga
     2941 aggtttttcc caccctctag gggtttcgtc aatagtgctc gaaacacagt gttttaccaa
     3001 aggagctgaa aggagaatta aatcaattct caaccccatc ttcctacgat aagctgctgc
     3061 tctatagtcc caccagctgt attgctcttc tagttgttca aatagcctaa agctatcgga
     3121 taagcctaga gataaaagag accgcaagca tgttctttct tgatcactgc agagcacctt
     3181 gtctttccat
//
LOCUS       DASCTH010000148         6826 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000207756, whole genome shotgun sequence.
ACCESSION   DASCTH010000148 DASCTH010000000
VERSION     DASCTH010000148.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000207756"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1880)
                     /locus_tag="QGH35_03135"
     CDS             complement(<1..1880)
                     /locus_tag="QGH35_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017044.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HJL81561.1"
                     /translation="MYTLRKLIFSLSLLLVIFPSIAAEEIPEINFEKFTLANGLRVIV
                     HEDHKIPVVAVNVWYHVGSKDEEIGKTGFAHLFEHLMFNGTEHYNNEYFGPFQKVGAT
                     DMNGTTNNDRTNYFENIPTSALELALWMESDRMGHLLGVVNQEKLDEQRGVVQNEKRQ
                     GENRPYGRVFLQAAKATFPSGHPYSWTTIGSMEDLNAATIDDVRKWFNTYYGPNNAVI
                     VLAGDINLETAKVLATKYFGDIPAGPAPLKKKRWIAKRTGQKREIMYDRVPNTRIYKV
                     WNTPELGSLEGVHLDLIATLLAGGKNSYLYQKLVYQERLATSVNAFNYGREIAGQFWI
                     TADLAKGRSLEELEAAIDQVVQDFTKRGPSKKLLDNTKTSTQASWIKGIQRVGGFGGK
                     SDILASGEIYLGDPKDYKQYMQSTLDSSATDLKGTAHKWLLDGEYVLTVAPIDSSSTV
                     ETKADRTQLPFPTDFPKLNLPEIQRAKLSNGLEVVLAERHDVPMINFSLQMKNGHAID
                     PDDQSGLASFTMSMLTEGTKSYDALKLSERLEELGTDLGTSTGLDVSSINLSTLKSNL
                     KPSLQVFREVIMEPVFDQNEIDRKKLIWLARIDQSLSDPNGIVGYVLPEIIFGQGHPY
                     AKP"
     gene            1961..3115
                     /locus_tag="QGH35_03140"
     CDS             1961..3115
                     /locus_tag="QGH35_03140"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HJL81562.1"
                     /translation="MKRLLGITIFSMAIIFSVHDASGENQSIQLSEAIDEMFVDRENK
                     PGCAVGVIKDGEYIHNSGYGMANLEYGIKIDSDSIFRIASISKQFTAMAIAIIEEKGL
                     LAFDDEMKKHIPDLMDYQSKVTINQMIHHFSGLGDYEDSDYPNRFKNAVDETFRWGSE
                     DYMSNNEFHEMIKTLPLIMEPETKFWYSNTGYALLALVAENVSSMSLRELAKKEIFEP
                     LGMDDSFFNDNVKLIVKNRADGYSPIKKHPGEYEINMTNLSWVGDGGIFTSINDFIKW
                     DQNFYDNKLGEGKASLIDTMEQTYAQTKVKKRNQKLSREQENQRTYAFAQNLAYHNGL
                     KRWSHSGSWVAYNSYYTRFPEIRFSVVVFCNTNEINATKVTNKIIDLYFE"
     gene            complement(3118..4269)
                     /locus_tag="QGH35_03145"
     CDS             complement(3118..4269)
                     /locus_tag="QGH35_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL81563.1"
                     /translation="MKLDLFDINALLTEEEQLVQVNVGRFVNDNVMPIIKECFENHIF
                     PAELIPDIASMGLLGSSIQGYDCAGMNAISYGLICKELERGDSSLRSFVSVQSSLVMY
                     PIDQYGSDEQKNYWLPKLAKGEAIGCFGLTESQGGSDPGNMKTHAKQDGNDWIINGSK
                     MWITNGSIADLALIWAMTEDGIKGFLIETDTEGFDAHEIENKFSLRASVTSELFFDNV
                     RIPNKNVLPNVTGLKGPLSCLTQARYGIAWGVIGAAEACLQELLDYTETRVLFGRSLS
                     ENQAIQIRLADMQRKITAASLIAYRLGQLKDQEKMNPTQVSLAKWNNTRMALDIARDC
                     RDMLGGAGISAEYVPIRHMLNLETVITYEGTETIHQLAVGRELTGKNAF"
     gene            complement(4288..4599)
                     /locus_tag="QGH35_03150"
     CDS             complement(4288..4599)
                     /locus_tag="QGH35_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014290269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HJL81564.1"
                     /translation="MTKKTFANFKEFYPFYINEHKLKYTKLTHFIGTWLVIFFTLRFL
                     MTIDPTFLAYAIISGYGCAWFGHFFIEKNKPATFKYPFYSLIGDFVMFAEILKGKHKI
                     L"
     gene            complement(4617..5666)
                     /locus_tag="QGH35_03155"
     CDS             complement(4617..5666)
                     /locus_tag="QGH35_03155"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016353818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HJL81565.1"
                     /translation="MNNQIHNINVIAKEKLPPPRILKAILPLTDAARETVLSSRKDLM
                     SILDRTDKRLIVVVGPCSIHDIEAAKDYANRLQELSIQLSDVFQILMRVYFEKPRTTI
                     GWKGLINDPTMDDTFQIDKGVHIARSLLIHLNETGLPIATEALDPIMPQYLGDLISWT
                     AIGARTTESQTHREIASGLSTAVGFKNGTDGSLSVAINAIKSSRESHHFLGLNEAGQI
                     TVYETKGNRYSHVVLRGGMQPNYDKSSVALCEKKLNEEGLPPRIMIDCSHGNSNKDFN
                     LQSSVAHNCIDQVLDSNQSIIGFMLESNINEGSQKISEDLSQLKYGVSLTDACMSWNS
                     TKTLLSDLAERLRMR"
     gene            complement(5780..6418)
                     /locus_tag="QGH35_03160"
     CDS             complement(5780..6418)
                     /locus_tag="QGH35_03160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007235121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJL81566.1"
                     /translation="MKLYDKYILPRILNCTCGLEPISEQRQKVVPHAKGNILEVGIGS
                     GLNFPFYETSKVNKIWGLDPSEELHEMAKKVVSGLDLDVEFLLKGAEEIPLIDDSVDT
                     VLITYTMCSIPEVEAAIKEIKRVIKPEGVMIFCEHGKAPDENTLKWQGRINPYWKKIS
                     GGCNLDRDIPNLIKSAGFSITQLETMYLPGTPKILGYHYWGFANIKGNECSS"
     gene            complement(6461..>6826)
                     /locus_tag="QGH35_03165"
     CDS             complement(6461..>6826)
                     /locus_tag="QGH35_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017004290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81567.1"
                     /translation="ALTLEYLGLIIIFLSYAVVESMNIALVLYILDNVFFSISFAIST
                     YFQKIADPADIASTAGISFTINHIAAVLLPALLGLIWIENHGAVFLIGASIAGASLIL
                     VRAIPDTPEIGKETYWTNN"
BASE COUNT         2072 a         1399 c         1218 g         2137 t
ORIGIN      
        1 ggtttagcat aagggtgtcc ttgaccgaaa ataatctctg gcaacacata accaactatg
       61 ccattaggat cagacaaaga ttgatcgatt cttgccagcc aaattaattt tttcctatca
      121 atttcattct gatcaaatac aggttccata atcacttcac gaaaaacctg taatgatggt
      181 tttaaattgg acttcaaagt agataggtta atgctggata catccagtcc ggtactggtg
      241 cctaaatcag ttcccagttc ttcaagacgt tcactaagct tgagtgcatc atagctctta
      301 gtcccttcgg tcagcataga catggtaaaa ctagctaacc cagattgatc gtcggggtca
      361 atcgcatgac catttttcat ctgtaaagag aaattgatca tcggtacgtc atgtctttct
      421 gccaatacaa cttccaaccc attactcaat ttcgcccttt ggatttcagg caaatttaac
      481 ttggggaagt cagtcgggaa aggaagttgg gtacgatcag ccttggtctc aactgtagaa
      541 gatgaatcaa tcggcgcaac cgttaataca tattccccat caagaagcca tttgtgcgcc
      601 gttcctttca ggtcagttgc acttgagtcc agcgttgatt gcatatattg cttataatct
      661 tttgggtctc ccaaatatat ttcgccgctt gctagaatat ccgatttacc gccaaagcct
      721 cctactcttt gtatgccctt gatccaagaa gcctgagtag aagttttagt gttatctaat
      781 aactttttgc tcggtcccct cttagtaaaa tcttgaacca cttgatcgat agcagcttct
      841 aattcttcta aacttcttcc tttggctagg tctgcagtaa tccaaaattg acctgcaatc
      901 tcccggccat aattaaatgc attaacgctg gttgccaatc tctcttggta tactaatttc
      961 tgatacagat aagaattctt ccctccggct agaagtgtgg caatcagatc tagatgcaca
     1021 ccttctaggg accctaattc tggggtgttc cacactttat atattcttgt attcggaacc
     1081 cgatcgtaca ttatctcccg tttttgtccc gttcttttag caatccatct ttttttctta
     1141 agaggagcag gtcctgctgg tatatcacca aaatatttgg tcgcaagtac ttttgctgtt
     1201 tctagattga tatcaccagc taggacgatc actgcattat tgggaccata gtaggtatta
     1261 aaccattttc gaacatcatc gatagtagcg gcattgaggt cctccatcga accaatagtt
     1321 gtccatgaat atgggtgacc actaggaaag gtggcttttg cagcttgcaa aaatactctg
     1381 ccatagggtc tgttctcacc ctggcgtttt tcattctgga caactcccct ctgctcatca
     1441 agtttttctt gattcacaac acctaagaga tgacccatcc ggtccgattc catccacaaa
     1501 gcgagctcca atgcagaagt cggaatattt tcgaaataat tggttcgatc gttattggtg
     1561 gtaccattca tgtctgtggc accaactttc tgaaagggac caaaatactc attgttataa
     1621 tgctcagtgc cattaaacat aagatgttca aacaaatgag caaaacctgt tttccctatt
     1681 tcttcgtcct tggatccaac gtggtaccaa acattcacag caacaactgg tattttatga
     1741 tcttcatgga caatgactct caaaccatta gctaaagtaa atttctcaaa gttaatttca
     1801 ggtatttcct ctgctgcaat tgatggaaag ataaccaaaa gtagagaaag agaaaaaata
     1861 agttttctta atgtgtacat aataatatat ttaacctaga tgttttttgt tgaaatgata
     1921 acggatttct aacaaattga taactatatt gggagaagaa atgaaacgct tactaggtat
     1981 taccatattt tcaatggcaa tcatcttttc tgttcacgat gcttctggag agaatcaatc
     2041 aattcaactt tctgaagcaa ttgatgaaat gtttgtcgac agggagaata aaccaggatg
     2101 tgctgttgga gtaattaaag acggagagta tatacacaat tcaggttatg gtatggctaa
     2161 ccttgagtat gggattaaga ttgattcaga ctcaatcttt agaatagcgt caatcagcaa
     2221 acaatttaca gcaatggcca tagcgatcat tgaagaaaaa ggtttactag cttttgatga
     2281 cgaaatgaaa aaacacattc ctgatctgat ggattatcaa agcaaagtga caatcaacca
     2341 gatgattcat cacttcagtg gtctcggtga ttacgaggac agtgattatc caaaccgttt
     2401 caaaaacgcg gttgatgaaa catttagatg gggcagcgaa gattacatga gtaataatga
     2461 atttcatgaa atgattaaaa cattaccgct aatcatggaa cctgaaacca aattttggta
     2521 cagcaatact ggttacgctc ttcttgcact ggtcgctgaa aatgtaagca gtatgagttt
     2581 gcgagaatta gctaaaaaag agatctttga gcctctcggc atggatgatt cttttttcaa
     2641 cgataacgtc aaactcatcg taaaaaacag agccgatggt tactccccta taaaaaaaca
     2701 tccaggcgaa tacgaaatca acatgactaa tctcagttgg gttggtgatg gtggtatctt
     2761 cacttctatc aatgatttta ttaaatggga tcaaaatttt tatgataata aactgggtga
     2821 aggaaaagca tccttgattg ataccatgga acaaacttac gctcagacaa aagtaaaaaa
     2881 aagaaatcaa aagctttcaa gagaacaaga aaatcagcgc acttatgcat ttgctcaaaa
     2941 ccttgcctac cataatggat tgaaaagatg gtcacactct ggctcatggg ttgcttacaa
     3001 tagctactat acgagatttc ctgagatacg tttttctgtt gtggtctttt gtaacaccaa
     3061 tgagattaat gccacaaagg tgactaataa aattatcgat ctatattttg aataaaatta
     3121 aaatgcattc ttgccggtta attctcgtcc tacagccaat tgatgaatgg tttctgtacc
     3181 ttcataagta atgacagtct ctaaattgag catgtgacga atgggaacat attccgcgct
     3241 gattccagct ccacctaaca tatcccgaca atctctagca atatccaaag ccattctcgt
     3301 attattccat ttagctaatg aaacttgggt tggattcatc ttttcctgat ctttaagttg
     3361 ccctagcctg taggcaatga gactggctgc tgtaatcttt ctttgcatgt ctgccaaccg
     3421 tatctgtatt gcttgattct ctgatagaga acgtccaaac aacactcggg tctctgtgta
     3481 gtccaagagt tcttgtaaac aggcttctgc tgctcctatt accccccaag cgataccata
     3541 tcttgcctgt gtgaggcaac tgagtggccc ttttaaaccg gtcacattgg gcagtacatt
     3601 tttattagga attctgacat tatcaaaaaa tagttctgag gtaaccgagg ccctcaaaga
     3661 aaatttattc tcaatttcgt gagcatcaaa gccttctgta tccgtctcta taagaaagcc
     3721 tttaatgcca tcctctgtca tagcccaaat caaagcgagg tcagctatag aaccgttggt
     3781 gatccacatt tttgaaccat ttataatcca atcattgcca tcctgtttgg catgggtttt
     3841 catattccca gggtctgatc ctccttgaga ttcagtcaat ccaaaacaac caatcgcctc
     3901 tcctttagct aattttggta gccaataatt tttttgctca tcacttccat attgatcaat
     3961 tggatacatc actaaactgc tttgaaccga cacaaaactt ctaagactgc tatcccctct
     4021 ttctaattct ttgcagatca aaccataact gatggcattc atgccagcac aatcgtaacc
     4081 ttgaattgag ctaccaagaa gtcccattga tgcgatatca gggattaatt cagctggaaa
     4141 gatatgattt tcaaaacatt ctttgataat gggcatcaca ttatcattaa caaatcgacc
     4201 cacattaact tgcaccaact gttcttcttc agtgaggagt gcattaatat caaataaatc
     4261 tagcttcata actaatgtag gactaaatta taggatctta tgttttcctt tcaggatttc
     4321 agcaaacata acaaaatctc cgatgaggct ataaaaagga tacttaaagg ttgcaggttt
     4381 gtttttttca ataaaaaaat gaccaaacca ggcacaacca tacccagaaa taatagcata
     4441 agctagaaat gtaggatcaa tggtcataag aaaacgtaga gtaaaaaaaa taactagcca
     4501 agtacctata aaatgagtga gcttcgtata tttaagtttg tgttcattga tgtagaacgg
     4561 ataaaactct ttaaaatttg caaatgtttt tttagtcatt ctttcatcct aacatatcat
     4621 cgcattctta accgctcagc aagatcactg agaagagtct tggtagaatt ccaactcata
     4681 caagcatcgg tcagtgaaac tccatatttt aattgcgata gatcttcaga gattttttga
     4741 ctgccttcat taatgttgct ttccagcatg aatccaataa tggattgatt ggaatccagc
     4801 acttgatcaa tacaattatg tgcaacgctt gattgtaaat taaaatcttt attcgagttc
     4861 ccatgactgc aatcaatcat gattcttgga ggcaaccctt cttcattaag ctttttctcg
     4921 caaagtgcaa cgctactctt atcataattc ggttgcatgc ctcctcttaa cacaacatgg
     4981 ctgtatcggt ttcccttggt ttcatagaca gtaatctgcc ctgcttcatt gagacctaag
     5041 aaatgatgcg attcccgtga cgatttaatg gcattgattg caacagataa agagccgtct
     5101 gtgccatttt taaagccaac agcagtggat agaccactgg caatctctcg atgagtttgt
     5161 gactcagttg ttcttgcacc aattgctgtc caactgatta aatctccaag gtattgcggc
     5221 ataatcgggt ccagagcttc ggttgctatt ggcaagccag tttcattcaa atgaattaat
     5281 agcgaacgtg ctatatgaac acccttatca atctggaatg tatcatccat agtaggatca
     5341 ttgattaaac ctttccagcc aattgttgtt cttggttttt caaaataaac acgcattaat
     5401 atttgaaaaa catcagataa ctggattgac aattcttgca atcgatttgc ataatcctta
     5461 gcagcttcaa tatcatgaat tgaacaaggt ccaaccacta caatcagtcg tttatctgtt
     5521 cggtctaaaa tggacattag gtctttacga ctactcaaga cagtttccct agcagcatct
     5581 gtcaacggca gaattgcttt caatatcctt ggaggaggca acttctcctt agcaataaca
     5641 ttaatattat gtatttggtt attcatattt tttaaaaaat agtagcggaa ctaactgatg
     5701 taaattggaa ccttactatt tcccactttt taaggttgta tagtgtaata aatccttggg
     5761 tataaatata tattagggat tagctgctgc actcattacc tttaatgtta gcaaaccccc
     5821 aatagtgata accgagtatc ttgggtgttc ccggcaaata catggtttcc agttgtgtga
     5881 tagagaatcc tgccgattta atgagattgg gtatatcccg atcaagatta caccctcccg
     5941 aaatcttttt ccaatatgga ttaattcttc cttgccactt cagtgtgttc tcatctggag
     6001 ccttaccatg ttcacaaaat atcatcactc cttctggttt tatgactctt ttgatttctt
     6061 tgattgctgc ttcaacttca ggaatcgaac acatagtgta ggttatcagt acggtatcta
     6121 ctgaatcatc gatgaggggt atttcctctg cccctttaag taaaaactca acatctaaat
     6181 ccaatcctga gacaactttc tttgccattt catggagttc ttcagacgga tcaagccccc
     6241 atattttatt gaccttagag gtttcataaa aaggaaaatt gagtccggag ccaattccaa
     6301 cttccagtat gtttccttta gcatgaggga caactttctg acgttgctca ctaatgggtt
     6361 ctaggccaca agtacaattt aatattctgg ggagaatgta tttatcatag agtttcatgt
     6421 tttccttgag tataaataca tgattaatct tcccttgaat ctagttattt gtccagtatg
     6481 tttccttgcc tatttcaggg gtatctggaa tggctctgac taagattagt gaagcccctg
     6541 caatacttgc ccctattaaa aaaactgcac catgattttc tatccaaatt aaccccagga
     6601 gagccggtaa taaaactgca gctatatggt taatagtaaa gctaatccct gcagttgaag
     6661 caatatcagc aggatcagct atcttttgga agtaagtact tatggcaaaa gatatagaga
     6721 aaaaaacgtt atctaaaatg tatagcacta gagctatatt catactttct acaactgcat
     6781 agctgaggaa tatgataatc aaacctaaat attctagagt gagggc
//
LOCUS       DASCTH010000149         4585 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000208505, whole genome shotgun sequence.
ACCESSION   DASCTH010000149 DASCTH010000000
VERSION     DASCTH010000149.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000208505"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="QGH35_03170"
     CDS             complement(<1..235)
                     /locus_tag="QGH35_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81568.1"
                     /translation="MQNNKSGQVVAAIVLSCAGVFMVFGMPSFVFGLISILEFSQSQA
                     NLISSAEIAGMALSSLLSILWIGRFNWRKVALCA"
     gene            complement(242..871)
                     /gene="nth"
                     /locus_tag="QGH35_03175"
     CDS             complement(242..871)
                     /gene="nth"
                     /locus_tag="QGH35_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HJL81569.1"
                     /translation="MKSKEAEQIFTILDQNNPNPTTELIYKTPFQLLVAVILSAQATD
                     VSVNKASKELFDLAETAEKMIQLGEIKLKNKIRSIGLFNTKAKNIIKTCKILIDHHQG
                     IVPDNRAELEALPGVGRKTANVILNTAFGKPTIAVDTHVFRVANRTGLATGKTPYIVE
                     QGLLEIIPKRFLKNAHHLLILHGRYVCISRKPLCYNCSVCSHCKFNNGG"
     gene            complement(889..2888)
                     /gene="metG"
                     /locus_tag="QGH35_03180"
                     /pseudo
     CDS             complement(889..2888)
                     /gene="metG"
                     /locus_tag="QGH35_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011233963.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
     gene            complement(2892..>4585)
                     /locus_tag="QGH35_03185"
     CDS             complement(2892..>4585)
                     /locus_tag="QGH35_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014513.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HJL81570.1"
                     /translation="IALLYIFKRPRWMMISMACCFLGLIVMTGFIGFVGWPVTVVSAN
                     FVALLLIFSLSITVHLTVRYRELNTQFPNKNQRWLVQQTLIDKFEPCLFTTITTMVGF
                     ASLLVAGIRPVIDFGWMMLISMGAIFIMVFLLFPVLLVLLKQKSTKQEKDFTSTITNA
                     FAKLVLRSPKTTSVIFLLIAIISVFGVTRLTSENQFIKAFKEDTEIYQGLSVIDNELG
                     GTTPLDIIIDADPNFFIEKQKSVETIEMDEFSDLDFFDDEEEIYEIGADSYWYNTFRL
                     KKITAIHNYLESLDEAGKVLSISTTLDVMKTLNDDEDLDTFFLSLLYKKIPLDVKEAL
                     FDPYLSQDGNQLRISFRVFESYPDLQRNVLLKKIKNELVNTYDLEESQVKLTGLLVLY
                     NNVLQSLIQSQILTIGFVFLAIMVMFLILFRSLKIAFIAIIPSMLASGSVLGLMGWLG
                     IPLDIMTITIASICIGIGVDHSIHYIHRFQLELQNQTPESAIERSHASTGKAIYYTSI
                     IIMVGFSILGLSNFIPTIYFGVFTAFAMLVALIANLTLLPLLLLRFNSDKFNTQT"
BASE COUNT         1377 a          848 c          853 g         1507 t
ORIGIN      
        1 aagcgcataa agctactttt ctccagttaa aacgacctat ccatagaatg cttagaaggg
       61 atgataatgc catgcctgca atttctgccg aggaaatcaa gtttgcttga gactgactaa
      121 actcaagtat agaaattaac ccaaatacaa atgaaggcat tccaaacacc ataaatacac
      181 ctgcacaact aagaactatt gctgcaacca cttgtcctga tttattattt tgcataatca
      241 gttaccctcc attattaaat ttacagtgtg aacaaacact acagttataa cacaaaggct
      301 ttcttgaaat acagacgtat cttccatgga gtatgagcaa atgatgagca ttttttagaa
      361 agcgttttgg gataatttct aataatcctt gttcaacgat atacggtgtt tttcctgtgg
      421 ctagacctgt tctgttggcc accctgaaaa catgggtgtc gacagcaatg gtaggtttcc
      481 caaatgccgt atttagaatg acgtttgctg tttttctgcc aactcctggc agcgcttcta
      541 gttctgctct gttatcagga acaattcctt ggtgatgatc gatgaggatc ttgcaggttt
      601 tgataatatt ttttgctttg gtattgaaca gtccaatact acggatctta ttttttaatt
      661 ttatttcgcc taattgaatc attttttcag ctgtctcggc taagtcgaac aattctttac
      721 tcgccttatt tacgctgaca tcggttgctt gtgcagagag tatgacggcg accagtaact
      781 ggaaaggtgt tttatagata agttcggtag ttgggttagg attattttgg tcaaggattg
      841 taaaaatctg ttctgcctct ttggatttca tgccttgcct atattttttt atctcactct
      901 catgcctgag attgctcctt cgtctgggct gattagaaat actccatcgt catcatttcc
      961 tgctgctaat aacatccctt cagagactcc gaaacgcatt gttcttggtt caagatttgc
     1021 cactatcaca atgttcctgt caatcaaact atcgggatca tagatgcctt tgattccagc
     1081 aaagattgtg cgttgttctc catcgaggtc aaccgtaatt tttatgagtt tgtctgcttc
     1141 agcaacatcc tcagcattaa ttacttttgc tactcttaaa tcaattttca tgaaatcatc
     1201 aaaattaacc atagttctct ccattcattt attgtgagtt atttagaagc atcaatcatt
     1261 gcatcaatgt tttctttttg tagccgagtc agtaagggct catagttatt aatttgatga
     1321 tcgatgagcg attgattgat gtccgaccag tgttgattgt cgagatctaa aaatgcctcg
     1381 gcttgttttg caattgctgg aacaactggt tttagataga tcatcaagat acggaataga
     1441 tttagaccca ttgtgcaaat ttcttgtact tgctcctttt ttgtcacatc tttagctaaa
     1501 ttccatggtt tttccttatc taagtatcga tttgcttgat ctgccagtga catgatggtt
     1561 ctcattgcac ggccaaattc tctgtgttta tacatcgttg ctatagatga tgatgattgt
     1621 tggaattgtg ataataattc ctgatcagct atcttgccgc ttaattggtt gttaaaatca
     1681 tcattgatga atttagcaca tcggcttgca atattgacgc atttaccaac cagatctgaa
     1741 tttactctgt ggagaaaatc atttgtatca agatcaaaat catccatgcc gctgttgagc
     1801 ttataagcat agtaatagcg gaggtattca ggattaagat gatctaggta agttcttgct
     1861 ttgatcattg ttcctcttga tttggacatt ttcttaccat tgacagttaa aaacccattg
     1921 acataaatac catcaggctt ttttaattca gctccatcca gtaccgcagg ccagaataat
     1981 gcatgaaaat aaatgatatc tttgccaata aaatggatca gttgattttc attgttggga
     2041 tcaacgtatg attggtagtc aaatgaattt tctttttgtt cacaaagatt cttcaaggag
     2101 gcaaaataac ccataggagc atccaaccaa acatagaaat atttgtcatc ttcctctggt
     2161 attttaaaac caaaataggg atcgtccctg gagatgtccc aattcttcag accgcttgtg
     2221 aaccattcat tcagtttgga tttgacagaa gggtgaatgt caatggtttg tatccactct
     2281 ttcagcattg actcagaatc ttttagcttt aaaaaataat gttcagattt cttttgactg
     2341 ggtttattac cggaaatcac tgaaattggg ttgatcagat ctgtgggttt ataggtggaa
     2401 ccgcaatcct cacagctatc gccatattga tcttctgcac cacactccgg acattggcct
     2461 ctaacatatc gatccggaag gaacatttgt tctttgtcgt caaaggcttg ttcgatgatc
     2521 tgtttttcaa tacggttatt ttttacaagt ttagaaaata tctcctcaat caattcttgg
     2581 ttttcttttg agtgggtagt gtagtaatta tcaaagttga tcagaaagtc tttgaagtcc
     2641 cggtgatgtt cttgcgtata ttcatttgca agttcctcag gatctacacc aattttttct
     2701 gctcttaaca taattggggt accatgcgca tcgctggcac agacataaag acagtctgtt
     2761 ccttgcagtt tctgaaaacg tacccaaatg tcagcttgaa tgtagcccac taaatggcct
     2821 atgtgtattg aatcatttgc atagggaagt gcgttggtaa ccaacaattt tttctgagca
     2881 ctatccattg tttatgtttg tgtattaaat ttatccgagt taaagcgtaa gagtaacaaa
     2941 ggtagcaacg ttaagtttgc tatcagtgct accaacatag caaaagccgt aaaaacacca
     3001 aagtaaattg ttgggataaa attcgaaagc cccaaaatgg agaatccgac cataataata
     3061 atggaagtgt aataaatggc tttgccagta gaggcatggg atctttctat ggctgattca
     3121 ggagtttgat tctgtaattc tagttggaaa cgatgaatgt aatgaataga gtgatcgact
     3181 cctataccga tacaaattga ggcaatcgtt atggtcataa tatccagtgg tatgcctaac
     3241 caacccatta atcctaaaac agatcctgaa gcaagcatgc ttggtatgat agcgatgaag
     3301 gcaattttta aggaacgaaa taaaatcaag aacatcacca tgattgctag aaaaacaaat
     3361 cctatagtta ggatttgtga ttggatcaag ctttgaagga cattattata cagcactagt
     3421 aaacctgtta atttgacttg agattcttcc aaatcatagg tatttactaa ctcatttttt
     3481 atcttcttaa gcaggacatt gcgttgcaaa tcaggataag attcaaacac tctaaaagag
     3541 atacgaagct gatttccatc ttgagataaa taagggtcaa acaatgcttc tttgacatct
     3601 aatgggattt ttttatacag taaagacaag aaaaatgtgt caagatcttc atcatcatta
     3661 agtgttttca tgacgtccaa agttgttgaa atggatagca ctttacctgc ttcatctaat
     3721 gattcgagat aattgtggat tgcagtaatc ttttttaatc gaaatgtgtt gtaccagtag
     3781 ctatctgcac cgatttcata aatttcctct tcatcatcaa aaaaatctaa atctgaaaat
     3841 tcatccatct cgatcgtttc aacagatttc tgtttttcaa taaaaaagtt agggtccgca
     3901 tcgataataa tatccagagg ggtcgttcct cctagttcat tatcaatgac tgagagtccc
     3961 tggtaaattt ctgtgtcttc tttaaaagct ttgatgaact gattttctga agttaatcga
     4021 gtaacgccga aaacactaat gattgcgatt aataaaaata tgacagatgt tgttttgggc
     4081 gatctcagta ccagtttagc gaaggcattg gtgatcgttg aggtaaaatc tttttcttgt
     4141 ttggtagatt tttgtttgag cagaaccaat aacacaggga agagtaagaa aaccataatg
     4201 aagatcgcac ccatactgat tagcatcatc catccaaaat caatgactgg tctaattcct
     4261 gcaaccagca atgaagcaaa gccgaccatt gtggtaatag tggtgaataa acagggttcg
     4321 aacttatcaa tgagcgtttg ttgaactaac cacctttgat ttttatttgg gaattgagta
     4381 tttagttcac gataacggac cgtcaaatga actgtgatag acaaactaaa gattaacaag
     4441 agtgcgacaa aattagccga tacaacagtt accggccaac ccacaaatcc aataaatcca
     4501 gtcataacaa tgagtcctaa aaagcaacag gccattgaga tcatcatcca tcttgggcgt
     4561 ttaaaaatat agagtagggc aataa
//
LOCUS       DASCTH010000150         2053 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000209659, whole genome shotgun sequence.
ACCESSION   DASCTH010000150 DASCTH010000000
VERSION     DASCTH010000150.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000209659"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /gene="pyk"
                     /locus_tag="QGH35_03190"
     CDS             <1..1379
                     /gene="pyk"
                     /locus_tag="QGH35_03190"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HJL81571.1"
                     /translation="VDAVRINLAHGSWDGDESNHRKTIKNIQKIARKINKPISILADL
                     KGNKLRVGDFKNEKINLVEGSEIIVRLKDENSITTSDEIHINSIKIMQLIEVGDQILL
                     DDGLIQIEVIGISKDASKASCKIIKGGELVSRKGFEVKDKTLKQQGLTDRDKKDLKKL
                     SECEVDWIALSFVNNETDVTQAKEILNSLGSNALVISKIERAAALEQLDEIINASDGI
                     MVARGDLALQVGSAHLTGLQKEIIEKTVVGKKIVITATQMMESMIHSRTPTRAEITDV
                     SNAVLDGTDAVMLSAETAIGEYPLETVSAMAEVCEGAEQYQQTLPETENTLISDFETI
                     DEAIAIASMRIAKKMNIKAIIALTETGSTAIMMSRLRYNFPIYAFTKSKYTQRRVSLC
                     RGVIPYPYIPTQGNAYELFLEISKRMLHTGEVKIGDMVVLTNGTITGISGQTNTLRIM
                     KITERKGK"
     gene            1444..>2053
                     /locus_tag="QGH35_03195"
     CDS             1444..>2053
                     /locus_tag="QGH35_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013333.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HJL81572.1"
                     /translation="MDHHRVGISGLATYLPPYRVDLSNWCDWTGNSKEKINYVIGSGF
                     RILGPNQSIYTMAANAVLQLIESYDINPSDIAYLALGTESSTDNSTGSVIIKGMINDE
                     LIKRKIDPISVNCEVPEFKQACLSGIYAVKNAIRFIKSDAPKSRAIVVCSDIALYQMG
                     TSGEPTQGSGAAAILIESNPKIAEVHTNISGSASNYRLIDFRK"
BASE COUNT          693 a          356 c          449 g          555 t
ORIGIN      
        1 aagtcgatgc tgtacgtatt aatttagctc atggcagctg ggatggtgat gaaagtaacc
       61 atcgaaaaac cattaagaac attcaaaaga ttgctaggaa aattaacaaa ccaatcagca
      121 ttttggcaga tttgaagggc aataaattaa gggtgggaga ttttaagaat gaaaaaatta
      181 acttggttga aggttctgag attattgtca ggttgaagga tgagaattcg ataacaacat
      241 cggatgaaat tcatatcaat tcaataaaaa tcatgcagct aatagaggta ggcgatcaga
      301 ttttattaga cgatggtcta attcaaattg aggtaattgg catttctaaa gatgcttcta
      361 aggcctcctg caagataata aaaggcggtg aattggtttc aaggaaaggc tttgaggtga
      421 aagataaaac gctgaaacaa caaggtttaa cggacagaga taagaaagat ctgaagaaac
      481 tgtcagaatg tgaagtggat tggattgctt tatcctttgt caataacgaa accgatgtta
      541 cccaggcaaa agagatattg aattcattgg gtagcaatgc cttggtgatt tctaaaatag
      601 aaagagcagc ggctctggag caacttgatg agattattaa tgccagtgac ggcattatgg
      661 ttgctagggg ggatctggct ttgcaggtag gctcagccca ccttactggt cttcaaaagg
      721 aaatcattga gaaaactgta gtggggaaaa aaattgttat tacagccacc caaatgatgg
      781 aatcaatgat tcacagtcga acaccaacga gagcagagat tacggatgtt tccaatgccg
      841 tactggatgg aacggatgca gtgatgctgt cggcagaaac agccataggc gaatacccat
      901 tagaaacggt aagtgccatg gcagaggttt gtgaaggcgc agaacaatac caacaaactt
      961 tacctgagac cgaaaatacg ttgatttcag attttgaaac tatcgacgaa gcaatcgcaa
     1021 ttgcttccat gcgtattgcc aagaaaatga atattaaagc aattattgca ctaacagaaa
     1081 caggctcaac agctataatg atgtctagac ttaggtacaa ttttcccatc tatgctttta
     1141 ccaagagtaa atacacccaa agaagagtca gcttatgtcg tggcgtgatt ccctatcctt
     1201 atataccaac ccagggcaat gcctatgaat tatttcttga gatctctaaa aggatgcttc
     1261 acaccgggga ggtcaaaatt ggcgatatgg ttgtcctcac caatggtact atcacaggta
     1321 tttcagggca gaccaataca ctcagaatta tgaaaattac agagagaaag gggaaataat
     1381 tctttcacaa ttaagcatta attaataaaa tcatctgctt cagaatcata gcttggaatt
     1441 tatatggatc atcacagagt tggaatcagc ggtcttgcaa cttacctccc cccataccgg
     1501 gttgatttgt ctaattggtg tgattggaca gggaactcaa aggagaaaat aaattacgtc
     1561 ataggttctg gattcaggat tctcggtcct aatcaaagta tttatactat ggctgcaaat
     1621 gcggtcctac agttaattga atcatacgat attaatccat ctgatattgc ttatcttgca
     1681 ttgggaacag aatccagtac cgacaattcc acaggctcag tcattatcaa aggaatgata
     1741 aatgatgagt taataaaaag aaaaatagat cccatttcgg tcaattgcga agtgccagaa
     1801 tttaagcaag cctgcttgtc aggtatttat gcggttaaaa acgcgattcg attcattaaa
     1861 tcagatgctc caaaatcaag agcaatcgtg gtatgttcgg atattgctct ctaccaaatg
     1921 ggtacgagtg gtgagccaac tcaaggttca ggtgcagcag ctatcttgat tgagtctaat
     1981 cctaaaattg ctgaagtaca tactaatata tctgggagtg cttctaatta ccgtctcatt
     2041 gatttcagga aac
//
LOCUS       DASCTH010000151         1632 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000211894, whole genome shotgun sequence.
ACCESSION   DASCTH010000151 DASCTH010000000
VERSION     DASCTH010000151.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000211894"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="QGH35_03200"
     CDS             complement(<1..526)
                     /locus_tag="QGH35_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014949953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HJL81573.1"
                     /translation="MRRVVVTGIGVLSSIGLNKESVKKSLMQGQSGIVYSQEFADAGI
                     RSQVAGSIDINLDEHIDRKLKRFMGDGSSYNYLAMKEAIEDSGLEKDHVSNTRTGLIM
                     GTGGGSPEAIVFGADALREKGKVSPFLVTRSMASGNSGTLSTAFEIKGVTYTLSSACA
                     TSAHCIGNAAELIQF"
     gene            complement(523..1044)
                     /gene="fabA"
                     /locus_tag="QGH35_03205"
     CDS             complement(523..1044)
                     /gene="fabA"
                     /locus_tag="QGH35_03205"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020023761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HJL81574.1"
                     /translation="MKRKNKYNLNELMACAKEMLFGPGNAQLPLPPMLMFDRITHISD
                     QGGDYGKGEIIAELDVKKDAWFFDCHFNKDPVMPGSLGVDAMWQLIGFFLGWIGGEGR
                     GRALGGGKIKFSGQVTPKTTILTYHLHIKRVMKSKLFLGVADATMKADNKIIYTGEDL
                     RVGLFQNLTEAQS"
     gene            complement(1087..>1632)
                     /locus_tag="QGH35_03210"
     CDS             complement(1087..>1632)
                     /locus_tag="QGH35_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HJL81575.1"
                     /translation="KRRKRRTRRIIFVLLFVAFATMLSWFFESQATTTVILTTHADVD
                     SNSSVNSGLSQRGFVTADALQELLNSVDVVAGVDAIYATPLRATQETAEPLMKSLDIP
                     ITLVEDVSAVEGLIETIMAKHKGKIILVVTHPDVLPKLVAELHGSKNIEPIDPSKDNK
                     LFIVSVPWFGKVKTLQLNYAF"
BASE COUNT          476 a          383 c          276 g          497 t
ORIGIN      
        1 caaattgaat cagttcagct gcattgccaa tacaatgtgc actggttgca catgctgaac
       61 taagtgtgta ggtcacgcct ttgatctcaa aggctgttga taaagtgcct gaattgccac
      121 ttgccattga tcttgttacc agaaatggac tgacctttcc tttttccctg agtgcgtcag
      181 caccaaaaac tatagcttca ggagaacctc caccggtccc cataatcaat ccggtccttg
      241 tattgctaac atggtctttt tctaatccag aatcttctat ggcttccttc attgccaagt
      301 agttgtaact ggaaccgtca cccataaatc gttttaactt tctatcaata tgttcatcga
      361 gattgatatc aatagatcca gcgacttggc tacgaattcc tgcatctgca aattcttgtg
      421 aataaacaat accggattga ccttgcatca aggatttttt caccgattct ttattaagcc
      481 cgattgagga gagaactcct atccctgtaa caactaccct tctcatgatt gggcttcagt
      541 tagattttga aaaaggccaa cacgtagatc ttcgccagtg tatatgatct tattatctgc
      601 cttcatagtc gcatcggcta ctcccagaaa aagcttagat ttcataactc ttttgatgtg
      661 caaatgataa gttaaaatag ttgtttttgg tgttacttgt ccggaaaatt taattttacc
      721 gccacctagg gctctgcctc taccctcgcc accgatccat cctagaaaga agccaatcaa
      781 ttgccacatg gcatcaaccc ctaatgaccc aggcataacc ggatctttat taaaatgaca
      841 atcaaaaaac cacgcatctt ttttaacatc cagctcggcg atgatctcac ctttaccata
      901 atctccacct tgatcactga tatgagtaat tcgatcaaac atcagcatag gtggtaaagg
      961 caactgcgca ttaccaggtc caaatagcat ttctttggca caggccatca attcgttgag
     1021 attgtattta ttttttctct tcattggtcc tattttaaca gaagagatac atgaaattga
     1081 ctaaagtcag aaggcataat ttaactgcaa agtcttcacc ttgccaaacc aaggcacact
     1141 cacgataaat aatttattat ctttactcgg atcaatgggt tcaatatttt tactaccatg
     1201 taattcagca accaacttcg gtaacacatc tggatgcgtc acaactaata ttattttgcc
     1261 tttgtgcttc gccatgatgg tttcaattaa cccctccact gccgaaacat cttctacaag
     1321 agtgattgga atatctagac tcttcattaa aggttccgct gtttcctgag tggccctcaa
     1381 tggtgtagca taaattgcat caacacctgc aacaacatca acagaattaa gtaactcttg
     1441 aagtgcatcg gctgtcacaa aacctcgttg gctcaaacca gaattgacac tcgagtttga
     1501 atcaacatct gcatgtgttg ttaatatcac tgtcgtggta gcttgcgatt caaaaaacca
     1561 agagagcatc gttgcaaaag caacgaaaag caaaacaaaa attattcgcc tggtgcgtct
     1621 tttccttctt tt
//
LOCUS       DASCTH010000152        12426 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000213140, whole genome shotgun sequence.
ACCESSION   DASCTH010000152 DASCTH010000000
VERSION     DASCTH010000152.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000213140"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="QGH35_03215"
     CDS             <1..620
                     /locus_tag="QGH35_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HJL81576.1"
                     /translation="KGCAQSLSALGATVWVTEIDPICALQAAMEGYRVVTMDDACSQA
                     DIFVTATGNYHVINKTHMEQMKDQSIVCNIGHFDNEIDVDCLQKLEWINIKPQVDHIV
                     FPDGKRLILLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWQNQDKYENKVYVLP
                     KALDEKVARLHLDKIGVQLTQLTKEQADYIGVSKEGPYKADYYRY"
     gene            648..1382
                     /locus_tag="QGH35_03220"
     CDS             648..1382
                     /locus_tag="QGH35_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024258.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HJL81577.1"
                     /translation="MISIIQITDPHLTRHKNTEIKSCNTNSSFERVLQNVVSNEQIDF
                     IIVSGDISDDGSRESYQFYKNTLESLKKPFVSIPGNHDNKDNFKLVFQKDFPNISCIP
                     LSDEWGLITLDSSVDDEEGGVLTDKQIKTAQDLIEASLHKNLILCLHHQPINMGFWID
                     EIGLQNKDQFLSLILDQPKVKAVVWGHVHHEYQTTLGSILFMSTPSTCYQFGEDETNQ
                     SRQKPGYRKIYLYDLGKLTTQVVRIN"
     gene            complement(1388..1978)
                     /locus_tag="QGH35_03225"
     CDS             complement(1388..1978)
                     /locus_tag="QGH35_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HJL81578.1"
                     /translation="MSEQNTPIFPLRTVLFPASRIPLRIFEPRYLDMVSSCLRSNTEF
                     GVVLSKEVIQPGMYETYNIGTLASIIDWSQDKDGLLNITVIGSNKFELISLSKKEDGL
                     NIADINILEQEKDFKAPPHFDNMIRLLEAILEDIDLFDDLDKQFESASWVSFRFAEIL
                     PLKIEDKQKCLELNDPLLRLNYLEPLLKLIRENSQQ"
     gene            complement(2002..3348)
                     /gene="mpl"
                     /locus_tag="QGH35_03230"
     CDS             complement(2002..3348)
                     /gene="mpl"
                     /locus_tag="QGH35_03230"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HJL81579.1"
                     /translation="MHIHILGICGTFMGSLAVLAKDLGFKVTGSDLNAYPPISDQLED
                     LNIEVIPNYDIEQLQQKPDLIVIGNVMSRGMPIIEEILRQGLAYTSGPLWLKENVLIN
                     KKVIAISGTHGKTTTASLITFLLKEIGIDPGFLIGGVSKDLKRSSNIGRDPIFIIEAD
                     EYDTAFFDKRSKFIHYQPDVLLINNIEFDHADIFKDTDQIIWQFHQLLRTLSPNTTIV
                     ANRDDHNIQQLFKMGVWSGIDYFSSKMDQRTKWACIEDDLGQYNLFCNDEKIDQMQLQ
                     LLGKHNMMNLTAAIAVLSNFELKPKDLIVGAKKFTGVKRRLEYLGQFSEIDLFDDFAH
                     HPTSIKASIAAVKSTLDSSSLYAVVELASNTMKKGSLKSELKKSFVDVDYAWILNSGN
                     IQWDIREEFAEIENVAILKTLVDLPDQVQSKIKPGDSLIIMTNSDSMRISNVLKKK"
     gene            complement(3355..3870)
                     /locus_tag="QGH35_03235"
     CDS             complement(3355..3870)
                     /locus_tag="QGH35_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81580.1"
                     /translation="MRKSGPEEVPDSNFLLVVTFIFFLLSQVLVGFKMYGGAVSLFFQ
                     LLFLDTVLLIIWLRAILQLSGKLERFRQSLTALFGTGTLFNFILSPFILSYAPYLEQL
                     TSSDELQITAELPMPALLSIVILIIAIWSVLVMGHIYSRATSKSRAIGIGLALMYSFI
                     NFMISTSLTVT"
     gene            3947..5062
                     /locus_tag="QGH35_03240"
     CDS             3947..5062
                     /locus_tag="QGH35_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013755.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HJL81581.1"
                     /translation="MKKIKNLPNFLETQESITSFASSILNTDRIGIDTEFIRTRTFFP
                     KLCLLQISSEQHSICIDVLKKNNLTLIWKEIFDPNKLKILHSARQDLEIFYLINKKIP
                     PNIFDTQIAASLLGYSSQIGIKQLLKQELDIEINKTETRSDWSKRPLNAKQIQYAIED
                     VKHLVSLHSVLKKKLIDKGRYDWVLEDSSKIMSNPKDFFDPHHSWKRISEIKNLRKKY
                     QNLAVKLAIWRETTAMEIDLPRQWALNDKSLIKIAKTKPNSLDQFNRLSIDTSNLKII
                     HQEQILNIISQDLNATDKSRTLKQKKRKLHLDPNLLKNFRKCIDEKALELNIPSDILA
                     SKKDCLSMLNDTNNSRLLNGWRKKVIGARLLKLLGNN"
     gene            complement(5059..5709)
                     /locus_tag="QGH35_03245"
     CDS             complement(5059..5709)
                     /locus_tag="QGH35_03245"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HJL81582.1"
                     /translation="MKIILLGAPGSGKGTQAEKLTHDFDLPQISTGNLLRQAIVDGSD
                     IGKKAKAIMDAGDLVSDEIVLELIKESLSDNSSRYGFVLDGYPRNINQAESLKALLNI
                     MDLQLDCTVLIDVDSEILIKRLSGRRICSKTGKTINIYFSKQSEIDECIEAGGELIQR
                     TDDNLESITNRINVYKKQTEPLIDYYQSDGLLKVVDGNGGIEVVYTRLTQAIGLQT"
     gene            5802..7064
                     /locus_tag="QGH35_03250"
     CDS             5802..7064
                     /locus_tag="QGH35_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014576136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HJL81583.1"
                     /translation="MANKNGFYAQSGGVTAVINASACGLIQTARKHHQQIGKIYAGRN
                     GILGALQEELIDTSLESDQDIDNLQYTPGGAFGSARYKLKSFEENQKEYKRLIEVFKA
                     HNIGYFFYNGGGDSMDTALKVSQIGKKMNFPITSVGIPKTVDNDLPITDTCPGFGSVA
                     KYIAVSTHEASLDVLSMAETSTKVFILEVMGRHAGWIAAASGLIANQENEPPHIILFP
                     EVPFEKKNFLKYVDKSVKDNGYCVIVASEGARYKNGKFLADAGTKDSFGHKQLGGVAP
                     IIASIVKDSLGYKYHYAIADYLQRSARHLASETDLKQAYSVGEAAVKIAIKGEKSKMV
                     TIDRVSDKPYKWKIGSVPLAEVANNEKMMPKEFITKNGFGITDACKTYMLPLIKGEAY
                     PSYKNGLPVFAKLKNKLVEKRLNKKFMV"
     gene            7131..9176
                     /locus_tag="QGH35_03255"
     CDS             7131..9176
                     /locus_tag="QGH35_03255"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HJL81584.1"
                     /translation="MSEAIALWIALTASVTAIVVGIVFSLGILKESDGNERMKEIAGA
                     IQEGATAYLNRQYLTIGIVGVVIFILLGFGVDWLSAIGFLIGAVFSALAGYVGMNISV
                     RANVRTAQAAYSGMSSALSVAFRGGAVTGLLVVGLGLLGISGYYLMLTSGLLGHEYTT
                     DESLHALIGLAFGGSLISIFARLGGGIFTKGADVGADLVGKIEAGIPEDDPRNPAVIA
                     DNVGDNVGDCAGMAADLFETYAVTIIATMLLGSLLFSGQEATNAVIYPLMLGSVSIVA
                     SVIGMLFVKSKEGGSVMGALYKGVIVSGIIAAIAFYPVTVNYMNMSIDLWYASLIGLG
                     LTAFMVFVTEYYTSTEYSPVQKIAEASKTGHATNIIAGLGVSMKATALPVIAVCLSIW
                     GAYECAELYGIAIAATSMLSLTGMIVALDAFGPITDNAGGIAEMAELPSEIRGITDPL
                     DAVGNTTKAVTKGYAIGSAGLAALVLFADFINNLPEGTEVKFSLQDPAVIIGLFIGGM
                     VPYLFGAMAMEAVGRAAGSVVDEVRRQFKEIPGIMDGTNKPDYSKAVDLLTKSAIKEM
                     IIPSMLPVVVPVALALIMNVMMPEDGGIRALGGLLIGTIVTGIFVAISMTVGGGAWDN
                     AKKYIEDGNVGGKGTEAHNASITGDTVGDPYKDTAGPAINPLIKIINIVALLLITVL"
     gene            9318..10712
                     /locus_tag="QGH35_03260"
     CDS             9318..10712
                     /locus_tag="QGH35_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HJL81585.1"
                     /translation="MDNKITKIISIITVLIMPVIANGASLMDIYADALINDPRLKEAY
                     ANKEAIIESKPQARSLLLPQISASGTYSDTNTDGSSTFQQRIVDPISGDFIVMTNTTE
                     FDQEADGLQWDITLRQNIIDPGAWMTVKKANKIVAQAEADYLAAEQDLMLRVCSAYFD
                     VLAAQDTLESQQAARTAIEKQLDQAKKRFEVGLIAITDVQEAQAAFDQSVANEISAKR
                     QLATAKEMLREITDSYPEKLSKPDANMPLVMPNPQSENDWVNTAMQQNLSLLSAQVGT
                     EIARSEVNIQRSGHYPTLGLQASKRDIDNNSLRSDSGGPYSPADSENTSDGIGLQLNI
                     PIYSGGQTSSRVRQAVAQHRSAKEKLERIARETTRKARDSYLGIISGIATVKALEQSV
                     KSSTIALQATEAGYEVGTRTTVDVLDARKRLFSAQTNLAISKYDYLKDMVKLKQAAGT
                     LSKADLIQINNWLQ"
     gene            complement(10714..12000)
                     /gene="waaA"
                     /locus_tag="QGH35_03265"
     CDS             complement(10714..12000)
                     /gene="waaA"
                     /locus_tag="QGH35_03265"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HJL81586.1"
                     /translation="MNIRALFRILYVIFSYLLLPLIFFHLVYKGYGDRDYFKRIGERF
                     GFNGDPLTESIIWVHAVSYGEVKAASILITELKRKYPEKKILITTYTPTGSSLVTELF
                     GDDVYHVYLPYDLADSMIRFFKWAQPEILIIIETELWPNLFHYCGQFNVPLVLASACV
                     SDRSIKLYKILLGLFQEAVAHGIVVGAQTEEDAKKFLLMGSDEARTFVTGNIKFDYHI
                     PENLTDRANLFKESLTSSRPIWVAGSTHSKEEEVILDAHRGILNHYPDLLLIIAPRRP
                     ERFNAVGNLITKKGFSSMKRSEQAKMNLDNVQVLLADVLGELPIFYSVSNVSFIGGSL
                     FHVGGHNLLEPASLNCPVITGPVLFGVEDIANMFLANDALKIIHNSDELSDLVSSLLS
                     DDKMRLSMIKGASNVIEANKGSLEKLLKMIEPLLES"
BASE COUNT         4139 a         2291 c         2286 g         3710 t
ORIGIN      
        1 gtaaaggctg tgcccaaagt ctcagtgcac ttggtgcaac tgtttgggtg acggaaatag
       61 atcctatctg tgccctgcaa gctgctatgg aaggatatcg tgtggtaact atggatgatg
      121 cctgttcaca agcagatatt tttgtaacag cgactggcaa ttatcatgtt atcaataaaa
      181 cacatatgga gcagatgaaa gatcagtcca ttgtttgtaa tattggtcat tttgacaatg
      241 aaattgatgt cgactgtctc caaaaattag aatggattaa cattaaaccc caggtcgatc
      301 atattgtttt ccccgatgga aaaagactga ttttgctagc agagggaaga ctggttaact
      361 taggatgtgc cacaggacat ccaagttttg ttatgtccaa ttcatttaca aatcaaacac
      421 tggctcaaat cgaattgtgg caaaatcaag acaagtacga aaacaaagtt tatgttttac
      481 ccaaggcact cgatgaaaag gtggcacgtc tgcacttaga taaaatcggt gtccagttaa
      541 cgcaattgac caaagaacag gctgattata ttggcgtttc aaaagaaggt ccctacaaag
      601 cagattacta ccgatattga ttatgtactg atatttttgg ttctttcttg attagcataa
      661 tacaaatcac ggacccgcat ttaacaagac acaaaaatac tgaaatcaaa tcatgcaaca
      721 ctaactcttc ctttgagagg gttctacaga atgttgtaag caatgaacaa atagatttta
      781 ttatcgtatc tggtgatatt tccgacgatg gtagtaggga aagttaccag ttttataaaa
      841 acacacttga atcattaaaa aaaccattcg tctccattcc tggaaaccat gacaataaag
      901 ataattttaa actcgtcttt caaaaagact ttcctaacat ttcatgcata ccgttatcag
      961 atgaatgggg gttaataacc ttggacagtt cagtagatga cgaagaagga ggtgtgttaa
     1021 ccgacaagca aatcaaaaca gcccaggatc tgatcgaggc tagtttgcat aaaaacctga
     1081 ttctttgttt acatcatcaa ccgattaata tgggattttg gattgatgaa ataggattac
     1141 aaaataagga tcaatttctt tcattgattt tagatcaacc aaaggtgaaa gcagtagttt
     1201 ggggacatgt ccatcatgag tatcaaacca ctctgggttc aattctattc atgtcaaccc
     1261 cttcaacatg ctatcaattt ggggaggacg aaactaatca atcaagacaa aaacctgggt
     1321 atagaaaaat ttatttatat gacttgggaa agctgacgac gcaagtagta cgaatcaatt
     1381 gacatgccta ttgttgtgag ttttctctga tcagtttcaa aagcggctct aagtaattca
     1441 atctcagtag tgggtcattc agttccagac atttttgttt gtcctctatt ttcaaaggca
     1501 aaatctctgc aaaacggaaa ctcacccagg atgcgctttc aaactgttta tctagatcat
     1561 caaatagatc tatatcttcc aaaatagcct caagaagtct gatcatgtta tcgaaatgag
     1621 gtggggcttt gaaatccttt tcctgttcca aaatatttat atcagcaata tttaagccgt
     1681 cttctttttt tgaaagagag atcaattcaa atttattgct accaattaca gtgatattga
     1741 gcagaccatc tttatcctga ctccaatcaa ttattgaagc taaagttcca atgttataag
     1801 tttcgtacat gcctggttgt ataacttctt tgcttagcac tactccaaac tctgtattag
     1861 aacgtaaaca actacttacc atgtctaaat acctgggttc aaaaattctc aatgggatcc
     1921 tagaagcagg aaataagact gttctaagtg ggaagatagg tgtattttgc tctgacattc
     1981 agaaaatctc tcccagataa atcacttttt tttcaaaacg ttgctaatcc tcatagaatc
     2041 gctattcgtc ataataataa gagaatcacc cggttttatt ttactttgta cttgatcagg
     2101 taaatcaaca agagttttca aaatagcaac gttctctatt tctgcaaact cctctctaat
     2161 atcccattga atatttccag aattcaatat ccatgcataa tcaacatcaa cgaaggactt
     2221 tttgagttct gattttaaac ttcctttttt catggtatta cttgccaatt caacaactgc
     2281 ataaaggcta gatgagtcta atgtggactt aactgctgca atagaagctt taatggatgt
     2341 agggtgatga gcaaaatcat caaaaagatc aatttccgag aactgaccta aatattccaa
     2401 tctcctttta actccagtga atttttttgc accaactatt aagtctttag gttttaattc
     2461 aaaatttgaa agcacagcaa ttgcagctgt taggttcatc atattatgct tccctaataa
     2521 ttgaagctgc atttgatcga tcttttcatc gttacaaaat aaattgtact gacctagatc
     2581 atcttcaata caagcccatt ttgttctctg atccatctta gatgaaaaat aatcaatacc
     2641 agaccaaact cccattttaa aaagttgttg gatattatga tcatctcgat tagcaacaat
     2701 tgttgtattt ggtgacaaag ttcttagcaa ttggtggaat tgccatatga tttgatctgt
     2761 atccttgaaa atatctgcat gatcaaattc aatgttatta attaataaga catccggttg
     2821 gtaatgtata aatttagatc gtttatcaaa aaatgctgtg tcatattcat cggcctcaat
     2881 aataaagatt gggtctcttc cgatatttga tgaccttttt aaatccttgg acactccccc
     2941 aattaaaaac cctggatcga ttcctatctc ttttagtaaa aaagtaatta aactcgccgt
     3001 tgtggttttg ccatgcgttc cacttattgc aataactttt ttattgatta aaacattttc
     3061 tttcaaccac agcggtccgg aagtataggc taagccttgc ctcaatattt cttcaataat
     3121 aggcataccc ctgctcataa cattgcctat gacaattaga tccggcttct gctgtaattg
     3181 ttcaatatca taatttggaa tgacttctat atttaaatct tccaactggt cgctaatcgg
     3241 agggtatgcg ttcagatctg aaccagtcac tttgaatcct aaatcttttg ctaacacagc
     3301 caaactgccc ataaatgtgc cgcaaattcc caagatgtgt atatgcataa tcttttaggt
     3361 aacggttagg gaagttgaaa tcatgaaatt aataaatgaa tacattagag ctaatcctat
     3421 gccgatggct ctactttttg aagttgccct agagtaaatg tgccccatta ccaagacaga
     3481 ccaaatcgct atgatcagta taactatact caatagtgca ggcattggta attcggcagt
     3541 gatttgtaat tcatctgagg aggttaactg ctctaaatag ggtgcataag aaagtatgaa
     3601 tgggcttagt atgaaattaa acagtgtccc agttccaaag agtgctgtta aactttgtct
     3661 aaatcgttcc agttttcctg ataactggag aatagctcgg agccaaatta ttaaaagtac
     3721 cgtgtcaaga aacaacaact gaaaaaagag acttaccgcc cctccataca tcttaaaacc
     3781 aactagaact tgagaaagta gaaaaaaaat aaaagtcacg actaatagaa aattggaatc
     3841 aggaacctct tcaggtccag actttctcaa aataatattc caaaatactg tcaataattt
     3901 aatcataaaa taggaatttc attataattg ttcaacgaaa gaatttatga aaaaaataaa
     3961 aaatttacca aactttctag aaacacaaga gtcaataact agttttgcct cctctatttt
     4021 aaatacagat agaattggca tagatacaga atttatcaga acaagaactt tcttccctaa
     4081 actttgtttg ctgcaaattt catctgaaca gcattcgatt tgtattgatg tactaaaaaa
     4141 gaacaattta acattaatct ggaaagaaat ttttgacccc aataaattaa aaatacttca
     4201 ttctgccagg caagacctag aaatctttta tttgattaat aaaaaaatac ctccgaatat
     4261 atttgataca caaattgctg cgtcactgct tggctattct tctcagattg gcatcaaaca
     4321 actactgaaa caagaactcg atattgaaat taataaaaca gaaacaagaa gcgactggtc
     4381 taaaagacct ctaaatgcaa aacaaataca atatgcaata gaagatgtga aacaccttgt
     4441 tagcttacat agtgttttga aaaagaaatt aatagataag ggacgctacg attgggtatt
     4501 ggaagattct tcaaaaatta tgtcaaatcc aaaagatttt tttgatccac accactcttg
     4561 gaaaagaatc tcagaaataa aaaaccttag aaaaaagtat cagaatttag cggtaaaact
     4621 agcaatttgg agagaaacaa cagcaatgga aattgaccta cctagacaat gggctttaaa
     4681 tgataaatct ttgataaaaa ttgccaaaac aaaaccaaat tcattagacc agtttaatag
     4741 acttagcatt gacaccagca atttaaaaat aatacatcaa gaacaaatac taaacataat
     4801 tagtcaggat ttaaatgcca cagacaaatc aagaactctt aagcaaaaaa agaggaaatt
     4861 acacctagat ccaaatctgt taaagaattt tagaaaatgt attgatgaaa aagcccttga
     4921 attaaatatc ccatcagata ttttagcttc gaaaaaggat tgcttatcca tgctgaatga
     4981 cactaataac tcaagattac taaatggttg gcgaaaaaaa gttattgggg caagactgtt
     5041 aaaacttcta ggtaataact aagtttgtaa acctattgct tgggttaatc gtgtatatac
     5101 gacttcaatc ccaccattcc catcaacgac tttaaggagt ccatcagatt gataataatc
     5161 aatcaaaggt tcagtttgct tcttatagac attaattctg ttagtaatcg attctaaatt
     5221 atcgtcagtt ctctgaatta attcacctcc cgcttcaata cattcatcaa tttcagattg
     5281 tttggaaaaa tatatattga tggttttgcc tgttttggag catatccgac gtccagaaag
     5341 ccgcttgatt aggatctctg agtctacgtc tattagcact gtacaatcga gctgtaaatc
     5401 cataatattg agtaatgctt tcaatgattc tgcttggttg atatttctcg ggtaaccatc
     5461 aagcacaaaa ccatatcggc tagaattatc ggataatgat tcttttatta gttcgagcac
     5521 tatctcgtcc gataccagat ctccggcatc catgattgct tttgcctttt tacctatatc
     5581 actaccatca acaattgcct gcctcaataa atttccagtt gaaatttgtg gtaaatcgaa
     5641 atcatgtgtc aatttctctg cttgagttcc tttacccgac cctggagctc ctaacaaaat
     5701 tatcttcata aagttatccc aacgattcta tgtggcaata tagtgttaat agaattaatc
     5761 taccgctatc atatataaac agtaaagata tggaaaagaa aatggcgaat aaaaacggat
     5821 tctatgcaca atctggaggg gttactgctg tcattaatgc tagcgcatgt ggcttgattc
     5881 agactgccag aaaacatcac cagcagatag gtaaaatcta tgctggtaga aacggtatac
     5941 tcggtgcctt acaagaggaa ttgattgata caagcctaga aagtgatcag gatattgata
     6001 atctgcaata cactccaggt ggagcctttg gttcagcgcg ttataaatta aaaagctttg
     6061 aggaaaatca aaaagaatat aaacggctaa tagaagtttt caaagcacac aatataggct
     6121 atttcttcta taacggcggt ggtgattcta tggatactgc cttaaaagtt tcccaaatag
     6181 gaaaaaaaat gaattttccc attacatcag ttggtattcc aaaaacagtg gacaatgatt
     6241 taccaatcac tgacacatgt cctgggtttg gctcggtggc aaaatacatt gcagtatcca
     6301 cacatgaagc tagcctggat gtgttgtcga tggctgaaac ttccacaaaa gtattcatac
     6361 tagaagtcat gggtcgtcat gctggctgga ttgcagctgc ttctggattg atagcaaacc
     6421 aagaaaacga accaccacat attatcctat ttccagaagt tccatttgag aaaaagaact
     6481 ttttgaaata tgttgataag tctgtaaaag ataatggcta ctgcgtgatt gttgcaagtg
     6541 agggagcacg ttataagaat ggaaaattct tagctgatgc tggaaccaaa gactcttttg
     6601 gtcataaaca actcggaggt gtagctccaa taattgcctc gatagttaag gattcactag
     6661 gctacaaata tcattatgct attgcagact atctccagcg atctgctaga cacttagcat
     6721 ccgaaacaga tctaaagcaa gcatattctg taggtgaagc tgcagttaaa attgcaatca
     6781 aaggtgaaaa atcaaagatg gtaactatcg atagagtatc agacaaacct tataagtgga
     6841 aaataggatc tgtgcctctt gcagaagtag ccaataatga aaagatgatg ccaaaggaat
     6901 ttataactaa aaatggtttc ggaattactg atgcatgcaa aacatacatg ctacctttaa
     6961 ttaaaggcga ggcctaccca agctacaaga acggtctccc tgtctttgcc aagttaaaaa
     7021 ataaattagt tgaaaagagg ttaaataaaa aatttatggt atgatcctca aaaaattttt
     7081 tatccctttt ataaaataac aaacacattc ttaaatttca ggagtaaaca atgagtgaag
     7141 ccatcgcatt atggatagca ttaaccgctt cagttacagc tatagttgtt ggaatcgtat
     7201 tctctttagg cattctaaaa gaatctgatg gaaatgaacg aatgaaagag atagctggtg
     7261 caattcaaga aggtgccacc gcttatttaa atagacagta tttaactata ggaattgtag
     7321 gagtagtaat ttttatattg ctaggttttg gagtagattg gttaagtgct attggatttc
     7381 ttattggtgc tgtcttttca gcactcgcag gatatgttgg aatgaatata tcagttcggg
     7441 caaacgtcag aactgctcaa gcagcctata gtggaatgag ttcagcttta tcagtcgctt
     7501 ttaggggtgg cgcagtaaca ggacttttag tagttggttt gggtttacta gggattagcg
     7561 gttattattt gatgctcaca tcaggtctat tgggtcacga atatacaact gatgaaagtc
     7621 tccatgcatt aataggatta gcttttggtg gttctttaat atcgattttt gctcgtttgg
     7681 gaggaggcat ttttactaaa ggagcagacg tcggtgcaga tttggttggg aaaattgagg
     7741 cgggtatccc tgaagatgac cccagaaatc cagcggtcat tgcagataat gtcggagata
     7801 atgttgggga ttgtgctggg atggcagcag accttttcga aacctatgca gtcactatta
     7861 ttgcaaccat gctactggga agcctacttt tcagcggtca ggaagctact aacgcggtga
     7921 tatatccatt aatgctaggg tccgtctcca tagttgcatc ggtaattggg atgctttttg
     7981 ttaagtctaa agagggcggt agtgtaatgg gagcacttta taaaggtgtt attgtttccg
     8041 gaattatcgc tgcaattgcc ttttatcctg tgactgttaa ttatatgaat atgtcaattg
     8101 acttatggta tgcctcacta attggtctag gtttgactgc ttttatggtc tttgtaactg
     8161 agtactacac atcaaccgag tacagtccag ttcaaaaaat tgctgaggct tctaagacag
     8221 gtcatgccac aaacataatc gctgggctag gtgtctcaat gaaagccacc gccttacctg
     8281 taatagcagt ttgtctgagt atttggggag cttatgagtg tgctgagctt tatggtattg
     8341 ctatagctgc tacctcaatg ctttctttaa ccgggatgat tgtcgcttta gatgcctttg
     8401 gtcctattac agacaatgca ggtggcattg ccgagatggc cgaattacct tcagaaatta
     8461 gagggatcac agaccctctt gatgcggttg gaaataccac aaaagccgta actaaaggtt
     8521 atgctatagg atctgctggc cttgcagctc tggtcttgtt tgcagatttt atcaacaact
     8581 taccagaagg cacagaagtc aaattcagtc tccaagatcc tgcggtgata ataggtctgt
     8641 ttataggggg catggtaccc tatttatttg gagccatggc tatggaagct gttggtaggg
     8701 ctgctggatc agttgtcgat gaggtaagac gacaattcaa agaaattcct ggaataatgg
     8761 atggtaccaa taagcctgac tattcaaaag cagtcgacct tcttacaaaa tcagcgatca
     8821 aagaaatgat tattccgtca atgttacctg tagtagttcc tgttgcgctt gctctaatca
     8881 tgaacgttat gatgcctgag gatggaggta taagagctct tgggggattg cttatcggaa
     8941 caattgtcac cgggattttt gtggcaatct caatgactgt tggtggcgga gcttgggaca
     9001 atgcaaaaaa atatattgaa gatggaaatg taggaggcaa aggaactgaa gctcataatg
     9061 cttcgataac cggagatact gtcggcgatc catataaaga tactgcagga cctgcgataa
     9121 atccgttaat aaaaattata aatatagtcg cactgctttt gattacggta ctttaagaaa
     9181 gttataccaa agttacgatt tgttacacca atcacttgta agaattagtc cgactatata
     9241 acctagagct tctcaaagaa tctgattctt atgagggctg agaataactg ataaaatcga
     9301 aaaattatcc aagaaaaatg gacaataaaa tcaccaaaat catatcgata ataactgttc
     9361 ttataatgcc agttattgcc aatggagctt ctctgatgga tatctatgca gatgcattga
     9421 ttaacgaccc tcgattgaaa gaagcttatg ccaacaaaga agcaattatt gaatctaaac
     9481 cgcaagcaag aagtttactg ttaccacaaa tatctgcatc cggaacttat tccgatacca
     9541 atactgacgg cagtagtact tttcaacaaa gaattgtgga tcctatttct ggtgatttta
     9601 tagtcatgac caacacaact gaatttgacc aggaagcaga cggcctgcaa tgggatatca
     9661 ctttgaggca aaacatcatt gatcctggcg catggatgac ggtaaaaaaa gcaaataaaa
     9721 tcgttgctca agctgaagca gattatttgg cagcagaaca agatttaatg ttaagagtgt
     9781 gcagtgctta ttttgatgtt ctggctgctc aggacacact ggaatcacaa caagctgcaa
     9841 ggacagcaat agagaaacaa cttgaccaag caaagaaaag atttgaagtt ggcttgatag
     9901 ctattaccga tgtccaagaa gcccaagcag cattcgatca atctgttgca aatgaaattt
     9961 cagcaaaaag acaactggca actgctaaag aaatgctaag agaaatcact gacagctacc
    10021 ccgagaagct ttcaaaacct gatgccaata tgcctttggt gatgccaaat ccacaaagtg
    10081 aaaacgattg ggtcaacaca gcaatgcaac aaaatttgag cctgctttca gctcaagttg
    10141 gcactgaaat tgcaagaagt gaagtcaaca ttcaacgatc tggtcactat ccaactttag
    10201 gtttgcaagc atccaagaga gatatagata ataatagttt acgttctgac agcggggggc
    10261 cctactcgcc tgcagacagt gaaaatacca gtgatggtat tggtctgcaa ttgaatattc
    10321 caatctacag tggcgggcaa acctcttcaa gagtcagaca agcagtggct caacatagat
    10381 ctgcaaaaga aaaattggaa agaatcgcta gagaaaccac cagaaaggcc agggactcct
    10441 acttaggaat catttcaggt atagctaccg tgaaagcttt ggaacaatca gtaaaatctt
    10501 caaccattgc tctgcaggca actgaggcag gatatgaagt aggaacgaga accacggtgg
    10561 atgttctaga tgcccgaaaa agactttttt cagcgcaaac aaatctagca atcagtaaat
    10621 acgattattt gaaagatatg gtaaagctta agcaagcagc tggaactctc tcaaaagcag
    10681 atttaataca gattaataac tggctccaat aaactagctt tctagtaacg gttcgatcat
    10741 ttttagtagt ttttccagtg atcccttatt ggcctcaata acattgctgg cacctttaat
    10801 catactgagc cgcattttat catctgataa caaagaagat accaaatcac ttaattcatc
    10861 agaattatga ataattttca aggcatcatt ggctaagaac atgtttgcaa tatcttcaac
    10921 tccaaataac actggtcctg tgatgactgg acaattcagt gaagcaggct ctaacaaatt
    10981 atgaccacca acatggaata aactcccccc aataaatgaa acattagata cggaataaaa
    11041 tattggtagt tctcctaaaa catctgccaa taggacttgc acattatcca aattcatctt
    11101 agcttgctca cttcttttca tggatgaaaa tcctttttta gtgattaagt ttccaactgc
    11161 attgaatcgt tctggacgtc ttggtgcaat aatcaacaaa agatcaggat agtgatttaa
    11221 aatgcctcta tgggcatcaa gaataacttc ttcttccttt gagtgagtac tgccagcaac
    11281 ccatatgggc ctactagatg tcagtgattc cttaaaaaga ttagctctat ctgtcagatt
    11341 ttcaggtata tgataatcaa atttaatatt cccggtaaca aatgttctcg cttcatctga
    11401 gcccattaac agaaacttct ttgcatcctc ttcagtctgt gcaccaacaa caattccatg
    11461 tgcaaccgct tcttgaaata gtcctaaaag aattttatac aatttgattg atcgatcaga
    11521 aacgcaggca ctagccaaca ctaaaggaac attaaattgc ccacaataat ggaataaatt
    11581 cggccaaagc tctgtttcta taatgatcaa gatctccggc tgtgcccatt tgaaaaatct
    11641 gatcattgaa tcagctaaat catatggaag atacacatga taaacatcgt caccaaatag
    11701 ttctgttacc aatgacgaac ctgttggtgt gtaggtagta atcagtattt tcttttcagg
    11761 atattttctc ttcaattcag tgatgagtat tgaagctgct tttacttcac cataggatac
    11821 tgcgtgtacc caaatgatgg attcggtcag aggatcgcca ttaaatccaa atcgctctcc
    11881 tatcctttta aaataatcac gatctccgta gcctttatag actagatgaa aaaatattaa
    11941 aggtagcaag agataggaaa aaataacata caatatccga aataaagctc ttatattcat
    12001 atacttataa attaaatttc tctaatttct ttaaattaaa tcactggttg atttaccttc
    12061 gataaaggat aacaatttaa cttgaccacc atgatcaata ttgatccaac attgcaacgc
    12121 ctatgacgat tgcattaatt gtggaaaaat cagggaattt cataccacca tgaatagttc
    12181 attcattatt cttgatctta tcatagagta attttttagc gggttaaaaa aaatcagaaa
    12241 aaaattaatg gtgcattaac cacattttga ctccccacaa ttaagacaag tcaaacagcc
    12301 atccatcata actgttgcaa cggtattaca gtggttacac atttgagctc cttcaggaaa
    12361 agattttttt acaaatgcgt cttgctgttt gtttctattc tcaaattctt gccttttttc
    12421 atccag
//
LOCUS       DASCTH010000153         5521 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000213825, whole genome shotgun sequence.
ACCESSION   DASCTH010000153 DASCTH010000000
VERSION     DASCTH010000153.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000213825"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            195..1262
                     /locus_tag="QGH35_03270"
     CDS             195..1262
                     /locus_tag="QGH35_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012993.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HJL81587.1"
                     /translation="MPSENKEKDAMVRADGTYSTAPLKKDMISIAIAQTRVQGVDGKN
                     PPKGIRKNLDYLLECIDITQGFGGRSDLICFHEFPITGFSNWSKEEHYKVAIEVPGVE
                     TEEIGKKCKEHGCYIVFGTYAKDPDWPEHILHLMVMMGPDGDVVAKHWKQRNVRGLFP
                     GSEQYTSSIYDVYDRFIEMYGQDQVIPVVQTDIGNIAMSAVQFEPELFRCMAFKGAEI
                     ICRVATGGFEYEDMRLTSYHNSLYTLIANNSINTGERNPGFFDDANYGGPGRSAIFGP
                     RGLEIAKAGVFETKIAASIPIKAFREKHRIPDLHLKLYQPVFDQYQERYDSGLYLKTL
                     PKNKQDAYKKFLENARWVHYW"
     gene            1303..2625
                     /locus_tag="QGH35_03275"
     CDS             1303..2625
                     /locus_tag="QGH35_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81588.1"
                     /translation="MNNKFGPIQLAPGISRFNVITYFYACFICIGVLAGMNFIQGYIL
                     TENLAIPKSNQGTISGDLAFIQEIIAILFVAFFGILADRIGRRSVMVFGTVVSAIGFA
                     LYPFATSTGELFAYRALFALGAAALASTIAIIVNDYPKEQSRAKFISMHSVLNALGVA
                     VFAFILGQLPKYFTNNGLDPVTSGRYTMIIVGIVFLISGLIFQLGLKGGTPEKLSQKP
                     SVKELVTVGIQSFKNPRIALSFGTTFLARADVSVNGIFISLWAIQSGAMMGLSAADSI
                     QLVVRVIVVTNLVSIVWAFIFGAILDRINRVSGMIVAMTLGTIAYGSMALIDSPLNYS
                     NLPFFILVSCSAIGTVIATSSLIGQEAPAKQRGSIIGISTLFGAIGILTATALGGRLF
                     DQLGPSGPFLFICGMHSVLLLATIIVRIRAPGMLFQSKLQPDEVHIND"
     gene            2618..3403
                     /locus_tag="QGH35_03280"
     CDS             2618..3403
                     /locus_tag="QGH35_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012770.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJL81589.1"
                     /translation="MIRKKYIDTSEGQCHIRFVEGEGLPIVFLHQVPSSSRMFEAIMN
                     QLGNEHSMYAIDTPGFGASFDPVNTPTMKEYCDWLLQIFEALSLEKLHLFGHHTGSCM
                     AVEIANEYPSLISSITMIGPFAATVEEKEVMRASMGADWSPVDDGSHLMMAWNLVGDI
                     LGARGHLELHHRETLDTLRAYHSVHQAHESVWEQDFISLFKQIDCPMLIMCAPDDVLY
                     PFFNVAKELRPDATYKEISGKNYETDLDSATIVDTFKTFMQNL"
     gene            3406..3927
                     /locus_tag="QGH35_03285"
     CDS             3406..3927
                     /locus_tag="QGH35_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81590.1"
                     /translation="MDHLQKTTVADKTSTLLSWLILVLFIVVFINMSVAPRVPIESPE
                     RASLRSFHLAIASILFAFSFLRVYIWWHFPPQNKQQKLPQEAFNQLHVLQFTLYCSFI
                     AQGLTGVVTAWSDGLIQFQSSSESVNHALWVFSGYLHSVFAFLYIAIILFIVLIGIYH
                     MIRYRVWRFNIFT"
     gene            3947..4474
                     /locus_tag="QGH35_03290"
     CDS             3947..4474
                     /locus_tag="QGH35_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81591.1"
                     /translation="MKIGKKKDWLLVLILLAVSCYLPYLIFGAYPLTNTTNATPDEIK
                     QLTANLDLPDYYLNELPMISDEQKQMEADAFSNWDGGKCRFCHSIKANDRDRMAPSLY
                     RIFAKPAGISDNFTYSDALIQMKNNGLIWTPEALDAFMSNPKDFIPGNRMRMEGIEDP
                     DTRKLIINYIMRESR"
     gene            4488..5357
                     /locus_tag="QGH35_03295"
     CDS             4488..5357
                     /locus_tag="QGH35_03295"
                     /inference="COORDINATES: protein motif:HMM:NF020468.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HJL81592.1"
                     /translation="MTIEFEHEINFQSNTWNRDAWARIQGDLNSENESISYVNGQILG
                     VKPGEAVKELCGFETFLCTRLLPQQEDGTIRRLNKEVIFYTNSRSGEIIHEWTNPWTE
                     EVVNVVHVANDPFNYTISEWLILAPEDFPGKEKQQPIKIPLLFPWRIIGDTVVLNTDM
                     HLYYPNALQPDKWVRESSGPMAQVSEMMRYFVSLDELQNPELTSVHYNGTWSRITPWL
                     PWMLMGDTPGHCVYVGAMIKSNSIDIIPKHIRQFAEENYPHMLSAPTEDYGPSISSLE
                     YYAREQTPAPPLK"
BASE COUNT         1623 a         1100 c         1149 g         1649 t
ORIGIN      
        1 ttttcccaca ctccacatta taacaataag attattttat gcgtttttgc ttacctgtac
       61 cgggctgaat tatgtcattc tcacttctct ttttgactgc aagattcaat gaatgtgaat
      121 aattaggccc aactacaaac tctgatatat atgctatagt ctcaaataat ttaatgtgtc
      181 ctaggagcag ctttatgccc tcagaaaaca aagagaaaga tgctatggtc cgtgcagatg
      241 gaacctactc cactgctcct ttaaaaaaag acatgatctc gatcgccatt gcccaaacca
      301 gagtccaggg tgtagatgga aaaaatcctc ctaaaggcat aagaaaaaac ttggactact
      361 tgctggaatg catcgatatt actcaaggct ttggtggtag aagtgatctt atatgctttc
      421 atgaattccc catcaccgga ttcagtaatt ggtctaagga agagcactac aaagtagcca
      481 tcgaggtccc gggagtagaa actgaagaaa taggaaaaaa atgcaaagaa cacggctgct
      541 atattgtttt cggaacatac gccaaggatc ctgattggcc tgaacacatc ctacacttaa
      601 tggtgatgat gggccctgat ggtgatgttg ttgcaaaaca ttggaaacag agaaatgtcc
      661 gtggcctatt tccaggatca gaacagtaca ccagttccat atatgatgtt tatgatcgtt
      721 ttatagaaat gtatggacaa gatcaggtga ttcctgttgt tcaaactgat attggaaaca
      781 tcgccatgtc ggctgttcaa tttgaacctg agttatttcg atgtatggcc tttaaggggg
      841 ctgaaattat ttgccgagtt gcaaccggtg gatttgaata tgaggatatg cgactcacct
      901 cctaccataa cagtctgtat acattaattg ccaacaactc aatcaatact ggggaacgca
      961 accccggttt ttttgacgat gcaaattatg gagggccagg gcgttctgcg atatttggtc
     1021 ctagaggctt agaaatagca aaggctgggg tttttgagac aaaaatagcg gcttccattc
     1081 caatcaaagc cttcagagaa aaacatcgaa ttccagattt gcatttgaaa ttataccaac
     1141 ctgtatttga tcaatatcag gaacgatatg attctggact gtatctaaaa accctaccta
     1201 aaaataaaca ggacgcatac aaaaaatttc tggaaaatgc tcgttgggtg cattactggt
     1261 aaatgattca taggcgattc ctactagagc atggagaaag atttgaacaa taaattcggc
     1321 ccaatacaat tggcaccagg aatctctcgg ttcaatgtca ttacatattt ctacgcctgc
     1381 tttatttgta ttggagtact ggctggaatg aacttcattc aaggatatat actcacggag
     1441 aatcttgcta tcccgaaatc caatcaagga accatcagtg gggatttagc ttttattcaa
     1501 gaaattatag caatattatt cgttgcattt tttggcatcc tagcagatcg gataggcagg
     1561 cgatcagtca tggtttttgg aacggtggtt tcggccattg gttttgcttt gtatcccttc
     1621 gcgacatcca caggtgagtt gttcgcgtat cgggccctat ttgctttagg tgcagctgca
     1681 cttgcctcaa cgattgcaat catcgtcaat gattatccta aggaacaatc aagggctaag
     1741 tttattagca tgcactcggt attgaatgcc ttaggtgttg ctgtcttcgc ttttatactg
     1801 ggtcaacttc ccaaatactt taccaacaac ggtctcgatc cagttacatc tggacgatat
     1861 acaatgatca ttgttggaat tgtattcctg atttctggac tgatttttca actgggatta
     1921 aaaggcggaa ctccggaaaa attgagtcag aaaccttctg taaaagagct ggttacagtc
     1981 ggcatacaat catttaagaa cccgagaatc gcactctcgt ttgggacaac gtttctggca
     2041 agagcagatg tttcagttaa tggcatattt atctcactat gggcgataca gagtggagca
     2101 atgatgggat tgtctgcagc cgattcgatc caactagttg tccgggttat tgttgtaacc
     2161 aatttagtga gtattgtttg ggcttttatt tttggcgcaa tacttgaccg tatcaatcgg
     2221 gtaagcggca tgattgtggc gatgactttg ggaaccattg cctatggttc tatggccttg
     2281 atcgattccc cattgaatta ttccaatcta cccttcttta ttctagtttc ctgcagtgcc
     2341 attgggaccg tcatagccac aagtagcctg atcgggcaag aagctcctgc taaacaacgg
     2401 ggttcgatta taggtatcag cacgcttttt ggagcaatag gcattctaac agcgaccgct
     2461 ttggggggtc gactatttga tcaactggga ccttcaggtc cattcctttt catatgtgga
     2521 atgcatagtg tgctactttt ggctacaatt attgtcagaa taagagcccc tggtatgctg
     2581 tttcaatcaa aattacaacc tgacgaggtg catataaatg attagaaaaa aatatattga
     2641 tacttctgag ggacagtgtc atatccgctt tgttgaaggt gaaggcttac cgattgtttt
     2701 cctgcatcaa gttcccagtt cttcaaggat gtttgaagca ataatgaatc aacttggaaa
     2761 tgaacactcc atgtatgcta ttgatacccc cggtttcgga gcaagttttg accctgtcaa
     2821 taccccaact atgaaagaat attgcgattg gttgctacaa atttttgaag ctctctcatt
     2881 ggaaaaactt cacctctttg gccaccacac aggatcctgt atggcagtcg aaattgccaa
     2941 tgaataccca agtttaatct cctcaataac catgatcggt ccctttgccg caacggtaga
     3001 agaaaaagag gtcatgcgag catctatggg tgcagattgg agtccagtcg atgacggcag
     3061 ccacttgatg atggcgtgga atttagtggg agatatcctt ggagcaagag gccaccttga
     3121 actgcaccac agagaaacgc tggacacctt gagagcttat cattcagtac accaagcaca
     3181 cgaatcggtt tgggagcagg acttcataag cctttttaag caaattgatt gccccatgct
     3241 gataatgtgt gccccagatg atgttttata tccgtttttt aacgtagcaa aagaactcag
     3301 accagatgca acctataaag aaatcagtgg caagaattat gaaactgact tagactcggc
     3361 aacgattgtc gacactttta aaacctttat gcaaaatctt taaacgtgga tcacttacaa
     3421 aaaacaacag tagcagataa gacatccacg ttattaagtt ggctaattct tgtactattc
     3481 attgtggttt tcatcaacat gtctgtggcc cctagagttc cgatcgaatc acctgaaagg
     3541 gcttcgttaa gaagttttca tttggcaatt gcttccatct tattcgcatt tagttttctc
     3601 agggtttata tttggtggca cttccctcct caaaacaaac aacaaaaatt accacaagaa
     3661 gctttcaatc aattgcatgt tcttcagttt actctttatt gttccttcat tgctcagggg
     3721 cttaccggtg tggttaccgc ttggtctgac gggttgatac aatttcaatc gagctcggaa
     3781 tctgttaatc atgctctgtg ggttttttct ggttatcttc acagtgtatt tgcattttta
     3841 tatatcgcta tcatcttgtt tattgtactc ataggtattt atcatatgat ccgttaccgg
     3901 gtttggcgat ttaatatttt cacttgatta ttaagatggg taagcaatga aaattggcaa
     3961 gaaaaaagac tggcttttgg ttttaatttt actagcagta tcttgttatt tgccctacct
     4021 aatatttggt gcctatccat tgaccaatac aaccaatgcc accccagatg agattaagca
     4081 attaactgcc aatctggatc tgccagatta ttatctcaat gaactaccaa tgatcagcga
     4141 tgaacaaaag caaatggaag cagatgcctt ttccaactgg gatgggggca agtgtagatt
     4201 ctgtcattca ataaaagcta atgatagaga tcgaatggct ccaagtttat atcgtatttt
     4261 tgctaaacct gcaggtatct ctgacaattt cacatattct gatgcactca tccagatgaa
     4321 aaataacggg ttgatctgga caccagaggc attggatgct ttcatgtcca atcctaaaga
     4381 ttttattcct ggaaatagga tgagaatgga aggaattgaa gatccagata caagaaaatt
     4441 aattattaat tacatcatgc gtgaatcgcg ttaacaagga gaaaagaatg accattgaat
     4501 ttgaacatga aataaatttt caatccaata catggaacag agacgcctgg gctagaatcc
     4561 agggtgattt aaactcagag aatgaatcaa ttagttatgt caatggccaa attcttggtg
     4621 ttaagcctgg tgaggctgta aaagaattgt gcggatttga aacatttttg tgtacccgac
     4681 tgctgcctca gcaagaggac ggtacgattc gtcgattaaa taaggaggtt attttctaca
     4741 ccaattctcg aagcggtgaa atcattcatg aatggaccaa tccctggaca gaagaagtgg
     4801 ttaatgtcgt tcacgtcgcc aatgatccct ttaattatac gatcagtgag tggctgatcc
     4861 ttgctcctga agattttcca ggaaaggaaa aacaacagcc aatcaaaatt cctctgcttt
     4921 tcccctggag aataataggt gataccgtgg ttttaaatac tgatatgcac ctttattatc
     4981 ccaatgcgct gcaacctgat aagtgggtca gagaatcatc aggtccaatg gctcaagtct
     5041 ctgaaatgat gcgctatttt gtatcattag acgaattaca aaatcctgaa ttaacttcag
     5101 tgcactacaa tggaacctgg agtcgcataa caccctggct accttggatg ttaatgggag
     5161 atacacctgg tcattgtgta tacgttggag ccatgattaa atcaaattca atcgatatca
     5221 ttccaaagca tatcaggcaa ttcgctgaag aaaattatcc acatatgctc agcgccccaa
     5281 cggaagatta cggacccagc atttccagtc tggaatacta tgcaagagaa caaactccgg
     5341 cacctccgct taagtaagaa agttaaaaaa agtgcatttt tttattaggt ttaaccggac
     5401 ggtttagacg aatatagctt ctttatattt gtcgaataat tccagcgtga tgattcccat
     5461 attgtcgtca gacgctttcg cttcaatggt gtcaataata cgcttttgta cctgctcggg
     5521 a
//
LOCUS       DASCTH010000154         2467 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000215764, whole genome shotgun sequence.
ACCESSION   DASCTH010000154 DASCTH010000000
VERSION     DASCTH010000154.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000215764"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="QGH35_03300"
     CDS             <1..1338
                     /locus_tag="QGH35_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019604.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJL81593.1"
                     /translation="NIIDEEFDVIVERGINLHLNHPIESMKELLEEHYDAIFVGTGAP
                     HGRELDLPGRHDSDQIHIGIAWLESVAFEHIDQVGKRVIILGGGNTAMDCCRTSLRLG
                     GKDVKVVYRGSRDRMKSSDWELEPALEENVELILNHTPEEYLVEDGQLVGVKFAHLKW
                     STDSDGRLNSEKIDEIIIPCDTVILAIGQENAFPWVERDIGLEFDQWEVPIVNPVTYE
                     STIEGIFFGGDAAFGPKNIIEAVEHGHQAAISIHKYCNDQSLEERLTNGMNLISTKMG
                     LHEWSYSNNFDTDERYEVPTVPMENRFKDINIEVELGFEPGQFQREVERCLNCDIQTD
                     FTTDLCIECDACVDVCPVNCLMITNNDEESVLRSKIKTPAENPEQAIFVSSKLPQTGR
                     VMIKDEDVCIHCGLCAERCPTAAWDMKKFELLIPYALDEENYPEDSNKVSGLK"
     gene            1419..>2467
                     /locus_tag="QGH35_03305"
     CDS             1419..>2467
                     /locus_tag="QGH35_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HJL81594.1"
                     /translation="MKSIFRMGIPVVAKNYFPSNIQGLPTWYEVRVSEHGYLAPSGEI
                     NIMVAMNAQTYEKDIKEIVPGGYLIYDSTWPRKELMIRKDVTVLGVPLAQMCNENFDT
                     ARARTLMKNTAYVGVLAALLEIEMEVIEQLFLDSFSSKVELVDLNKKALQLGFDYAQS
                     NFSCPASFTTKRIDKTDGYIMIDGNTAAALGCLYAGATVGAWYPITPSTSLMDSFLRY
                     CKQYRIDEETGKNNYIIIQAEDELAAIGMALGAGWNGARSFTPTSGPGISLMSELLGY
                     GYYAEIPTVVFNVQRTGPSTGMPTRTQQADILSCAYASHGDTKHVCLYPANPEETFEF
                     SKTAFDLAEQLQTPV"
BASE COUNT          779 a          420 c          546 g          722 t
ORIGIN      
        1 aatattatcg atgaagaatt tgatgtcatt gttgagcgtg gaatcaatct ccatttaaac
       61 catcctatag aaagtatgaa agagttactg gaggaacact atgatgccat ttttgttggc
      121 accggtgctc cacatggcag agagttagat ttacctggta gacatgatag tgaccaaatc
      181 catattggca tagcttggtt agaatcagta gcttttgaac atatagatca agtgggtaaa
      241 agggtcatca ttttaggcgg cggcaatact gcgatggatt gttgtagaac ctctctcagg
      301 ttaggtggca aagacgtcaa agtagtttat cgtggcagta gagatcgaat gaaatcttcg
      361 gattgggaat tagaacccgc gttggaagaa aatgttgagt taatcttgaa tcataccccg
      421 gaagagtatt tggttgaaga tggtcaatta gtgggtgtga aatttgccca tcttaaatgg
      481 tctactgatt ctgatggcag gttgaactct gaaaaaatag atgagattat cattccttgt
      541 gacacagtta tccttgccat tggccaagaa aatgcattcc catgggtaga aagagatatc
      601 ggtttagagt ttgatcaatg ggaagttcca attgttaacc ctgtaacata tgaatccact
      661 attgagggta tcttttttgg tggtgatgca gcatttggcc caaaaaacat aattgaagca
      721 gttgaacatg gacatcaagc ggcaatttca atccataaat attgtaatga ccagtcgttg
      781 gaggaacgtt tgactaatgg catgaatctc atttcaacaa aaatgggact tcatgagtgg
      841 agctatagta ataattttga tactgacgaa cgttatgagg tcccaacggt tccaatggaa
      901 aataggttta aagatattaa tattgaagtg gaattaggat ttgaaccagg acaatttcag
      961 cgtgaagtag aacggtgtct gaactgtgat atacaaactg attttaccac tgatttatgc
     1021 atagagtgtg atgcgtgcgt ggatgtttgc cctgtcaatt gtctgatgat tactaataac
     1081 gatgaagaaa gtgtgcttcg aagtaaaatt aaaacacctg ctgagaatcc tgaacaagct
     1141 atttttgtct cctccaaatt gccgcaaacc ggtcgggtta tgatcaaaga tgaagacgtt
     1201 tgtatccatt gtggtttatg tgccgaacgt tgtcccactg ccgcatggga tatgaaaaaa
     1261 tttgaattac ttattcccta cgctttagac gaagagaatt atccagaaga cagcaataaa
     1321 gtaagcggtt taaaatgaat ccagcaaatc ataataattt tacagtgaaa tttgcgaatg
     1381 tgaatggaac tggttcgtct agtgctaata agctcttcat gaaaagtatc ttcagaatgg
     1441 gcatacccgt tgtcgcaaaa aattattttc cttcaaatat acaaggcttg ccaacttggt
     1501 acgaagttag ggtaagcgag catggctatc ttgcaccttc aggtgagata aacatcatgg
     1561 tcgcaatgaa tgcacaaact tatgaaaaag atattaaaga gatcgtacct ggaggttatt
     1621 taatttacga ctcaacatgg ccaagaaaag agttaatgat aagaaaggat gttaccgttt
     1681 tgggcgtgcc cttagcacaa atgtgcaatg aaaattttga tactgctaga gccaggacat
     1741 taatgaaaaa taccgcatat gtcggtgttt tagccgcatt attagaaata gaaatggaag
     1801 ttattgagca gctttttctg gattctttta gttccaaagt agagctggtt gacttaaata
     1861 aaaaggcact acaattaggt tttgattatg cacaatctaa tttttcatgt cctgcttctt
     1921 ttacaacaaa aagaatcgat aaaacagatg gatacattat gatcgatggc aatactgcag
     1981 ctgcactagg ttgtctgtat gctggggcaa ctgttggggc atggtatcca atcaccccat
     2041 ccacttcctt gatggattct tttttgcgct attgcaagca atatcgcata gatgaagaaa
     2101 ccggtaaaaa taattacatc atcatccaag ctgaagacga attggcagcc attggtatgg
     2161 cattaggtgc aggatggaat ggtgcgagat cttttacacc aaccagtggg ccaggaattt
     2221 cgttgatgag tgagttactt gggtatggct actatgctga aataccaact gttgtattta
     2281 atgtacaaag aacaggacct tctacaggca tgccaacaag aacccaacaa gctgatattt
     2341 tatcgtgtgc ttatgcctct cacggggata ccaaacatgt ctgtttatac cctgcaaatc
     2401 cggaagaaac atttgaattt tccaaaacag ctttcgactt agctgagcaa ttacagaccc
     2461 ctgtcat
//
LOCUS       DASCTH010000155         1607 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000220571, whole genome shotgun sequence.
ACCESSION   DASCTH010000155 DASCTH010000000
VERSION     DASCTH010000155.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000220571"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="QGH35_03310"
     CDS             complement(<1..1128)
                     /locus_tag="QGH35_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81595.1"
                     /translation="MNKQFFVVLLVLLLQVSSCTSSKTIQYENIPLQRAQIELAEAQL
                     LDIGILIFDPNLPKEEQELIFPEVRKAEARYMPYHLKTTLEDTGFWGGIWVLPEKSEA
                     MDLIVSGRVEISNGLKVAVRIGVWDITGREWINKVYETTVPQSAYSKRRDHTQDPYQN
                     LYNKISNDLLEIKNLLSLAELQRISEIGDLRFAAELIPDVYEDYLEKNSKDIISAIRL
                     PSENDAMMERIRSVKEREFILVDTLNEYYAKLYQDISGPYENWRKLSREEILTYEELQ
                     RSARTRKLLGAAAILGAVVYDGDSQASSTMKQVAVYGGMEAVKSGMGKTAEAKVHKES
                     LKELGESFDIQAQPLIIEIEGQTLRLTGTAKEKFLEWRKLLR"
     gene            complement(1156..>1607)
                     /locus_tag="QGH35_03315"
     CDS             complement(1156..>1607)
                     /locus_tag="QGH35_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81596.1"
                     /translation="SMSGSDSAASVFDESLGVFDQEMASERIVIASSGEGSDGMSAQE
                     AADASAVESGLLEGEIPMENASGESSIGEATDQDQDEASSQQQNDSNESSHQSEQMKE
                     RIPDDIPMDGTGEDVIARQIREAAIQEDDPEIREALWDEYRKHMGIR"
BASE COUNT          432 a          385 c          251 g          539 t
ORIGIN      
        1 tctcaataat ttacgccatt ctaagaattt ctctttagca gttcctgtca atctaagcgt
       61 ctgtccttca atttctatga tcaagggctg tgcctgaatg tcaaaagatt cgcctaattc
      121 cttaagagat tctttatgaa ctttcgcttc agccgtctta cccataccac ttttaactgc
      181 ttccattcca ccatacactg caacttgttt cattgtgctg ctcgcttgac tatccccgtc
      241 gtagactaca gctccaagta tggcagctgc acccaacagc tttctagttc ttgcacttct
      301 ttgtaattcc tcataggtca gtatttcttc acgagaaagt tttcgccaat tttcataagg
      361 gcctgagata tcctgatata actttgcata gtactcattc aaagtatcca caagaataaa
      421 ctcacgttct ttgacgctcc ttatcctctc catcattgcg tcattttctg aaggcaatcg
      481 tattgcacta ataatatctt tgctgttctt ttcgagatag tcttcataaa cgtctggaat
      541 taactcagca gcaaacctta agtcacctat ttcactgatt ctttgtagct ctgctaagga
      601 cagcaagttt ttaatttcta aaagatcatt agaaatttta ttgtacaggt tttgatatgg
      661 atcttgagta tgatctcgtc ttttactgta agcactctgg ggaacagttg tttcatagac
      721 tttattaatc cactccctac cagttatatc ccaaacgcca attctaaccg ccaccttaag
      781 accgttggat atctctactc tacccgagac aattaagtcc attgcttctg atttctctgg
      841 taaaacccaa atgccacccc aaaaaccagt gtcttccaaa gttgttttaa gatgataagg
      901 catataccta gcctcggcct ttctaacttc tggaaaaatc agttcttgct cttctttagg
      961 taaattggga tcaaaaatta aaataccaat atctagtagt tgtgcttctg caagttcaat
     1021 ttgagcacgt tgaagaggta tattttcata ctggatcgtt tttgaagaag tacagcttga
     1081 aacctgtaat aacaagacaa gcaacaccac aaagaattgc ttattcatca accggctaaa
     1141 cataatttca taattttatc taattcccat gtgttttcta tactcatccc acaatgcttc
     1201 tcgaatttct ggatcatcct cttggatagc tgcctctctg atttgtcgag caatcacatc
     1261 ctctccggta ccatccatgg gaatatcatc ggggattctt tccttcattt gttccgattg
     1321 atggctggac tcatttgaat cattttgttg ttgagaagaa gcttcatctt ggtcttgatc
     1381 agtggcttca cctatggaag attcgcctga agcattttcc atgggtattt ctccttccaa
     1441 aagacctgat tcaaccgctg atgcatctgc agcttcctga gcactcattc catctgatcc
     1501 ttcaccggat gaagcaatca ctatcctttc acttgccatt tcttgatcaa aaactcccaa
     1561 tgattcgtca aatacactgg cagcagaatc actaccagac attgaac
//
LOCUS       DASCTH010000156         3872 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000224375, whole genome shotgun sequence.
ACCESSION   DASCTH010000156 DASCTH010000000
VERSION     DASCTH010000156.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000224375"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="QGH35_03320"
     CDS             complement(<1..254)
                     /locus_tag="QGH35_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018917250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HJL81597.1"
                     /translation="MCGVVGIVAKQSVNQEIYNALTVLQHRGQDSSGIMTDDNGVLCQ
                     RKSNGLVADVFTNKHMLQLRGNIGIGHVRYPTAGSLSEVEA"
     gene            complement(286..825)
                     /locus_tag="QGH35_03325"
     CDS             complement(286..825)
                     /locus_tag="QGH35_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014704.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HJL81598.1"
                     /translation="MINHIVFPYLYFPINIFGSEEIVSILDFALVITLVISTVVGFFR
                     GFVSEILSLLVWVMAFWATFSFDNTLSVYFISSIESEASRVWLSRLLILVAVLLIGGI
                     VNKLLSKIVSWKFSGNLFFGTLFGFFRGLVLITIIILILEDTRFYSEPWVQDAMFLEY
                     AENISDFFYNLFLEQYNQI"
     gene            complement(878..2143)
                     /locus_tag="QGH35_03330"
     CDS             complement(878..2143)
                     /locus_tag="QGH35_03330"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HJL81599.1"
                     /translation="MENWGLSEWLHWQETLNSEEIDLDLERIHLVASRLEIESPIDRT
                     FLIAGTNGKGTTVALIEELLVNKKLRVGSYTSPHLIDYNERICVDKKPVDNARLIDSF
                     KLIESVRGNVALTFFEFGTLSAFTIFNDFECDAWVIEVGLGGHLDATNIITPSISIIT
                     NVDLDHQNWLGNTIDEIAKEKAGIISPGVPCIFGDTPVPSIIQTVADSEGSDLYVWNS
                     DFFLLDNGDSFQWKGRNTEIDSIIMPSHWGPGECNNLALSLTSIEIMDSELLPSAKEL
                     NHLLSNFSVPGRFEIIEQKQTWILDVAHNPHAAKNLKKKLESLNSCKKITVILSMMQD
                     KDVIGFVKILDDLVEQWIVCEMDTPRSHSAHALQKKLIDYGITNVSAESGLIEAFSNI
                     KIRTEKNDRILITGSFEIVGPAKRWLDSE"
     gene            complement(2177..3049)
                     /gene="accD"
                     /locus_tag="QGH35_03335"
     CDS             complement(2177..3049)
                     /gene="accD"
                     /locus_tag="QGH35_03335"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HJL81600.1"
                     /translation="MSWFEKIVPSRIKTKKRQRSKSVPEGVWINCPECKSQLYKAEIE
                     RNFSVCIKCNHHMKIGARDRLNQFLDQDSHHELFSKITSVDPLNFKDTKKYKDRLIAS
                     QKASGENNALLVMEGTLSQIGVVVCAFEFKFLGGSMGSAVGEKFVRAVDRSIYKNAPL
                     ICFSSSGGARMQEAFLSLMQLAKTTSALVDLAAARIPFISVLTDPTTGGVSASIAMLG
                     DINIAEPGALIGFAGPRVIQQTVGEKLPEGFQRSQFLLEKGALDMIVDRREMRETITG
                     LIQKLTQAESAKQQ"
     gene            complement(3065..>3872)
                     /gene="truA"
                     /locus_tag="QGH35_03340"
     CDS             complement(3065..>3872)
                     /gene="truA"
                     /locus_tag="QGH35_03340"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HJL81601.1"
                     /translation="MPSLTKRIACGVEYFGFNYSGWQSQQQSSSVQDHIETAISTVAN
                     EPIRIFASGRTDAGVHAKCQVFHFDTKSNRTPSEWLYGVNSHLPDDINIIWVKGVVQE
                     FDARYSAISRTYQYLIVNQKINDVFLINHALWVPYNLDINLMQQAARHFHGEHDFTSF
                     RSSSCQARSPTRTISTLNIKQSNQRILIQCTANAFLQHMVRNIVGTLIDIGEGVLSPE
                     SIPIILDSKDRIKSGKTASSSGLYLVGIDYPGKFQLPHSEFKVRLESYLS"
BASE COUNT         1244 a          789 c          598 g         1241 t
ORIGIN      
        1 gcttcaactt cacttaatga ccctgcagtt ggatagcgaa cgtgtccaat gcctatattg
       61 ccccgcaatt gcaacatgtg tttattagta aaaacatcag caaccaagcc gttgcttttc
      121 ctttgacaca agacgccatt atcatcggtc ataatacctg atgaatcttg acctctgtgt
      181 tgcaacacag tcaatgcatt gtaaatctct tgattcactg attgctttgc aactataccg
      241 actactccac acataacgat tttttttcct tttagacctt aaaggttaaa tttgattata
      301 ttgttcaaga aataaattat aaaaaaaatc agagatattt tctgcatatt ctaagaacat
      361 tgcatcctgc acccaaggtt cactataaaa tctagtatct tcaagaatca aaataatgat
      421 agttataaga accaagcctc taaagaaacc aaatagtgtg ccaaaaaata aatttccaga
      481 aaatttccaa cttacaatct ttgataacaa tttattaact attcccccaa ttaatagaac
      541 ggctactagt atcagtaaac gggataacca aactcgagaa gcttctgact caatcgaaga
      601 aataaaatag acactcaggg tattgtcaaa actgaaagta gcccaaaatg ccataaccca
      661 aaccagtaag gaaagaattt ctgatacaaa gcctctaaaa aatccaacta ctgtagaaat
      721 caccaaagtg atcacaagag caaaatccaa aattgatact atctcttcgg aaccaaaaat
      781 atttattgga aaatataagt aaggaaaaac aatgtgattt atcattattg catagctgtt
      841 aaaacaaaaa acttattaat tttctctcat aagcgaatta ttctgagtcc aaccacctct
      901 ttgctggacc cacgatctca aaggaaccag ttatcagtat cctatcattt ttttcagtac
      961 gaattttaat attgctgaat gcttcaataa gtcctgattc agcactaacg ttagtaatgc
     1021 cataatcaat taatttcttt tgaagtgcat gtgccgaatg agatcttggt gtatccattt
     1081 cacaaactat ccactgttca actaggtcat ctaaaatttt tacaaaacca ataacgtctt
     1141 tgtcttgcat catgctcaaa atgacagtaa tttttttgca tgagttcaaa gattctagtt
     1201 tcttcttcag attctttgca gcgtgagggt tatgagctac atcaagaatc catgtttgtt
     1261 tttgctcaat gatctcaaat cttcctggca cggaaaaatt acttaataga tggttcaatt
     1321 cttttgcact tggtaataat tctgaatcca ttatttcaat agaggtcaat gacaaagcta
     1381 agttattaca ttctcctgga ccccaatgag atggcatgat tatagaatca atctcggtat
     1441 ttctcccttt ccattgaaaa ctatctccat tatccaataa gaagaaatca ctattccata
     1501 catacaaatc agaaccttct gaatcagcaa cagtctggat aatagaaggt acgggtgtat
     1561 ctccaaaaat acagggaaca ccaggactga taatgcctgc tttctcttta gcaatctcgt
     1621 ctatggtgtt gcctaaccaa ttctggtgat ctaaatcaac atttgtaatt attgagattg
     1681 agggtgtaat gatattggtt gcatctaaat gaccaccgag ccctacttct atcacccaag
     1741 catcacattc gaaatcatta aatattgtaa atgctgataa ggttccaaat tcaaaaaaag
     1801 taagcgccac attacctcta accgattcga tcaatttaaa tgaatcaatt aaccttgcat
     1861 tatctacggg tttcttatct acacaaatcc gttcgttgta atcaatcaag tgaggtgatg
     1921 tgtagctgcc cactcgcaat tttttattaa ctaatagttc ttctattaat gctacggtag
     1981 ttcctttacc attggtccca gcaattaaaa atgttctatc aattggcgac tcaatttcta
     2041 gccttgatgc cacaagatga atgcgttcca ggtctagatc tatttcttct gaatttagtg
     2101 tttcctgcca atggagccac tcggacagtc cccaattttc catagataat gattacctaa
     2161 taaataacaa tgaatactac tgttgttttg ctgattcagc ttgtgttaat ttctgaatca
     2221 gacctgttat ggtttctctc atttcccttc gatcaacaat catatctaat gcccctttct
     2281 ctaggagaaa ttgactcctc tgaaaacctt ctggcagttt ttctcctact gtttgttgaa
     2341 taactctagg acctgcaaat ccaattaatg caccgggttc agctatatta atatctccca
     2401 acattgctat gcttgcagaa acaccaccag tagttggatc agttaaaaca gaaataaacg
     2461 ggattcttgc tgcagccaaa tcaactaagg ctgaggttgt ttttgctaat tgcattaatg
     2521 acaaaaatgc ttcttgcata cgtgcacctc cgctggaact aaaacaaatc aagggagcat
     2581 ttttatatat actacggtca actgctctca caaatttttc tccaacagca gaacccattg
     2641 aaccgccaag aaatttaaac tcaaaagcac agaccacaac cccaatctga ctcaaagttc
     2701 cttccataac cagtaaagca ttattctcac cactagcttt ctgagatgcg atcagtctgt
     2761 ctttatattt ttttgtgtct ttaaaattta agggatcaac agaggtaatt ttagaaaata
     2821 attcatgatg ggaatcttga tccaaaaatt gattcagtcg atctctggca ccaattttca
     2881 tatggtggtt acatttaatg caaacagaaa aatttctttc aatttctgct ttatataatt
     2941 gagatttaca ttctgggcaa ttgatccaaa caccctcagg cactgattta gagcgttgcc
     3001 ttttttttgt cttaattctc gacggaacta ttttttcaaa ccaactcata tttattttat
     3061 ttagctaact taaataactc tccaatctta ccttaaactc tgaatgaggt aattgaaatt
     3121 tacctggata atctatgccc acaaggtaca acccagatga cgaagcagtt ttaccagatt
     3181 ttattctgtc ttttgaatcc aatatgatcg gaatactctc tggcgacaaa accccttcgc
     3241 ctatatcaat taaagttccg acaatatttc ttaccatgtg ttgcagaaaa gcatttgcgg
     3301 tacattgaat gagtattctt tgattgcttt gttttatatt taaagtcgaa atagtgcgag
     3361 ttggactcct ggcctggcag ctgctcgatc taaaagaagt gaaatcatgc tctccatgga
     3421 aatgacgtgc tgcttgttgc attaagttta tatctagatt gtaaggaacc caaagagcat
     3481 gattaatcaa aaaaacatca ttgatttttt gatttacaat aaggtattga taggttcttg
     3541 aaatcgctga gtatcgcgca tcaaattcct gtacaacacc tttaacccat atgatattaa
     3601 tatcatccgg caaatgtgaa ttgactccat acagccactc acttggggtt cggtttgatt
     3661 tggtatcgaa atgaaaaact tggcattttg catgaactcc agcatctgtt cgaccggagg
     3721 caaatattct tataggttca tttgcaactg tagagattgc agtctcaata tgatcctgaa
     3781 ctgaactgga ttgttgttgt gattgccaac ctgagtaatt aaaaccaaag tattccacac
     3841 cacatgcaat cctttttgtt aatgagggca tt
//
LOCUS       DASCTH010000157         6743 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000225906, whole genome shotgun sequence.
ACCESSION   DASCTH010000157 DASCTH010000000
VERSION     DASCTH010000157.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000225906"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="QGH35_03345"
     CDS             <1..200
                     /locus_tag="QGH35_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HJL81602.1"
                     /translation="FYYVRVLENPTCRWSTWDAIKAGYKPRSGLHQTIQERAWTSPIW
                     YIPQKGDLDIIPLGGTFRTVN"
     gene            226..1026
                     /locus_tag="QGH35_03350"
     CDS             226..1026
                     /locus_tag="QGH35_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HJL81603.1"
                     /translation="MRTRILIFFIVGLLIFFLDVAFNSDESDKDIYISDQELTSLITA
                     WKSQVGRNPTDEEIVKIINNLVEEEILYREALSLGLDKEDRIIKRRLAQKITFLKQET
                     LPENPNQEILEQFYNEHKERYFTKPSYSFTHLFFAQDTDSERRSIQALQDLLDDKAPI
                     DSDPYFLGKNFTDKTIEEIARNFGNDFIVAFDHLESNQWSGPFESSFGHHLVLLRDYK
                     DGFFPSFNTIKDQVLNDYFTQIKENAVEQYINDVKSEYRIIINPKLEF"
     gene            1035..1982
                     /locus_tag="QGH35_03355"
     CDS             1035..1982
                     /locus_tag="QGH35_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020037491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HJL81604.1"
                     /translation="MRLSGLLCLLIVAPQLSAHEFNPAHLVIDETAENKYQVNWMYPI
                     KNIGPRAEIIVPSTCSSKAQSPYKQGKYLVEKIDLLCGESLKGQTIEVTNLSVLTDAL
                     VTITYLNKDVFEGLMNLKESKLLVPVQQQSFPSSYFGLGVDHLLSGTDHILFILGLLF
                     LVTGIMNMIKTITAFTIAHSITLGLSVLDLIALPRATVEAVIALTIIFLALEISEKRI
                     YKSTPWLIAFAFGLLHGLGFANALTGIGIANEQLLLSLLFFNFGIEAGQLLMIPIFGV
                     LIWLAYKFNKYNQSATCVSYILGAMGFFWLINRTVGIIY"
     gene            2016..2774
                     /locus_tag="QGH35_03360"
     CDS             2016..2774
                     /locus_tag="QGH35_03360"
                     /inference="COORDINATES: protein motif:HMM:NF039614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HJL81605.1"
                     /translation="MTREINAAMNSCELTFLTRDKIDMDLAKQQHQQYQSVLSSLGCE
                     IVIVPTESNLADSVFIEDTAVVLDEVAVLCRPATALRGQEVAGVEDVLQQYRTLISIQ
                     SPGTLEGGDLLRVGKVIYAGLSTRSNQNGIEQLSRIVADYGFSVKTVETEKCLHLKSA
                     ISEVAPGVLLINPDWISRSTFKNYELIDVDKEEAHAANALLVGQKIIYPSSFPRTLEK
                     LVNAGLDVTQVNVSELQKAEGAVTCCSLIFKMSL"
     gene            2893..6066
                     /locus_tag="QGH35_03365"
     CDS             2893..6066
                     /locus_tag="QGH35_03365"
                     /inference="COORDINATES: protein motif:HMM:NF027232.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HJL81606.1"
                     /translation="MNHNQGNYPMRSVKTTIVVMVMSLFFGSSNCFAMDTVDPELLSA
                     MEYRLVGPHRGGRVTTVAGVPDKPQLYYMGATGGGVWKTENAGLTWENISDGFFNVGT
                     IGAIAVAESDHNVIYVGTGEKSIRGVTTSQGDGIYKSTDAGATWMHIGLPKAGQISRI
                     KIHPTNPDIAYAGAQGQIWGPNKERGVYRTMDGGKTWEQVLSVDAQTGATDLRMDPTN
                     PRILYAAMWEHGRKPWYVLSGGTAGGIFKSTDAGDNWEKLTNGLPELIGKIGVDVSAS
                     NPNRLYAIVEAMPGEGGLYRSDDGGTSWELKNKSRILWTRSWYYMHIAADPVNEDIVY
                     VLNAPFMKSIDGGVTFEKKSTPHGDHHDHWINPYNNKNMINGNDGGATITFDGGESWS
                     SIMNQPTAQFYRINTDNQFPYRIYAGQQDNSTVAILSQTYGNSIGNDDFYPVGGGESA
                     HIAFDPDNPRLIYATSINSTLTEYDADNERIREIKPYPEFIFGMESKDLRYRTNWNAP
                     VTASPQDPSVIYYGTEKLLKTTDRGVTFEQISPDLTKNEKDKQGLNGGPITAENVGAE
                     FYGTILTITASPHEYGTVWVGSDDGLIHVTRNDGESWNEVTPQDLRGAMINSIEVSPH
                     DPGTAYVAVAGYKMNDFRPYIYKLTDFGRQSRRIDRGLPDDNFIRVVREDPERQGLLF
                     AGSEVSLYVSFDDGDNWQSLALNLPPVPITDLAIRQNDLVVSTQGRALWVLDDLSALR
                     QVQNDMISKPLHVFTPGPVEMIKGSNPSEPSKTKGTNPLRGVVFNYHIRDEQEDPLTI
                     EITDNNGNHVRTYSSEESDFERCIIANIDQRLPFKVKYPPKEQGANKWVWDMRRHGLH
                     CIDDIKLFEGFAGAYVMPGTYQARISIGGAEDTVTLNLITDRRITADSHAIAEVEQKV
                     FDLTALMNELLDSLTSIRKSRTQIKTLMTDFPDAELLQDSGQSAIDRLSAWEREVLQV
                     DYETYEDEDNLPGKLVKQVRHLLDVIDDAGPPIAAGALERLRDLEDEWANLQAELRDI
                     STSDIEAVNNWASTNTIPHISPPIARR"
     gene            6124..>6743
                     /locus_tag="QGH35_03370"
     CDS             6124..>6743
                     /locus_tag="QGH35_03370"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81607.1"
                     /translation="MQQRSKKIPIIIIIVCILIVVLSIYLEGNKKAEKIGPDLIVFNA
                     DIRTSDPKSPIAQAFAVNKGKFIAIGTNQEIQSLGKKNTQQIDAQGFSVLPGFIDSHT
                     HLSSGGKIVTGVNLTGVKEKSVWLDMIAEKVKTMEPGEWLLGGRWDYTLENKGLPTRW
                     ELDEVSPHNPVALSDIDGHSMWVNTLAIEKAKITTDSIVPVGGQIML"
BASE COUNT         2004 a         1304 c         1489 g         1946 t
ORIGIN      
        1 ctttctacta tgttagagtt ttggaaaatc caacctgtcg atggtcaacc tgggatgcta
       61 ttaaagcagg ctataaacct agatcgggtt tacaccaaac gatacaggaa agggcatgga
      121 cttcacctat ttggtacatc ccacaaaaag gtgatttaga tataatccca ttaggaggaa
      181 cctttagaac agtgaattaa tatttagttc taacttccac taaaattgcg tacaagaata
      241 ttaattttct ttatagtcgg attactgatc ttttttcttg atgtcgcctt caattctgat
      301 gaaagtgata aagatattta tatctctgac caagaattaa ccagccttat taccgcctgg
      361 aaatctcaag taggcagaaa tcctacagat gaagagattg ttaaaataat caacaatcta
      421 gttgaagagg aaattctcta tagagaggct ctatcattgg gactggataa agaagatcgg
      481 atcatcaaga gaagactggc tcagaagata acattcttaa aacaagaaac tcttcctgaa
      541 aatcccaatc aagaaatttt agaacaattt tataatgaac ataaagagcg gtattttacc
      601 aagcctagct atagctttac ccatctattt tttgcacaag acactgattc tgaaagaagg
      661 tctatacaag cattacaaga cttattggac gacaaagcac cgatagattc agatccttat
      721 tttctaggta aaaacttcac agacaaaacc attgaggaga ttgctagaaa ctttggcaat
      781 gatttcatag tggcttttga tcacttggag tcaaatcagt ggtctgggcc ttttgaatca
      841 tcctttggac accacttagt tttattgaga gattataagg atggtttttt tccgtctttt
      901 aatacaatca aagatcaagt actcaatgat tattttaccc aaatcaaaga gaatgccgta
      961 gagcaatata ttaatgatgt taaatctgag tacagaatca ttattaatcc aaaattagaa
     1021 ttttgatgtt tagtttgagg ctgtcaggtt tactgtgttt acttattgtt gccccacaac
     1081 tgagtgcaca tgagtttaat ccagcccacc ttgtcataga tgaaactgca gaaaataaat
     1141 atcaagttaa ttggatgtat ccgatcaaaa atatcggccc aagagctgag attattgttc
     1201 ctagcacttg ttcaagcaaa gcccaatccc cttacaagca aggtaagtat ttagtcgaga
     1261 agatcgattt actttgtggc gaatccctaa aaggtcaaac gatagaagtc acaaatctga
     1321 gcgtgcttac agatgctctg gtgaccatta cttaccttaa taaggatgta tttgagggct
     1381 taatgaacct taaagaatca aagcttctgg tacctgtaca acagcagtct ttcccatcaa
     1441 gttactttgg tcttggtgtc gatcatcttc ttagtggcac agatcatatt ctttttatcc
     1501 tcggtctgtt gtttcttgtg accggtatta tgaatatgat taaaacaata actgcattta
     1561 cgattgccca tagtattact ttagggcttt ctgtactgga tctaatagct ttacctcgag
     1621 ctactgttga ggctgtcatt gctttgacta taatcttttt agctctcgaa attagtgaga
     1681 aaagaatcta caaatcaaca ccctggctta ttgcatttgc atttggctta ttacacggct
     1741 taggttttgc taacgcatta actggaatag gtatcgcgaa cgagcaatta ctgctctctt
     1801 tactcttttt taactttggt attgaagccg gccagttatt aatgattcct atttttggtg
     1861 ttttaatttg gttggcttat aaattcaata aatacaatca atcagctacc tgtgtttcat
     1921 acatattagg tgcaatgggt tttttctggc ttattaatag aactgttgga ataatttact
     1981 aaaaatagag aggagacaaa attgtgctga cagctatgac acgtgagata aatgctgcca
     2041 tgaacagttg tgaattgact tttttaacca gagataagat cgacatggat cttgcgaaac
     2101 aacaacatca acagtatcaa tcagtattgt cttcactcgg atgcgagatc gtcattgtgc
     2161 cgaccgaatc aaatttggca gattctgtat ttatcgagga tacagcggtg gttctagatg
     2221 aagttgcagt gttatgccgg ccggcgacag cattaagagg acaggaggtt gcaggtgttg
     2281 aggatgttct gcaacaatat agaacactta tctcaattca gtctcctggt actctcgaag
     2341 gcggtgacct gcttcgtgtc ggtaaagtca tttatgccgg tctctccaca cgatcgaatc
     2401 aaaatggtat cgaacaattg agtcgcattg ttgcggacta cggattttcc gtgaaaacag
     2461 tagaaacaga aaagtgtctg catttaaaat ccgctatttc agaagttgcc cctggcgtgc
     2521 ttctcattaa tcctgactgg attagtaggt ctacttttaa gaattatgaa ttgattgatg
     2581 tcgacaagga agaagcgcac gctgccaacg cacttcttgt cggtcaaaag ataatttatc
     2641 cctcttcatt cccacgcacc ctggaaaaac tggtgaatgc tggtctcgat gtgacacagg
     2701 tcaacgtgtc tgaactgcaa aaggctgaag gtgcagttac ttgctgtagc ttgattttca
     2761 aaatgtcttt gtaattgcta tttgatagtt tgattatttt aatcaacgaa caattcttct
     2821 caaacggcaa cggtaatctc agtaatcaat agtctttgta agaacggggt ggattaggta
     2881 aattagataa atatgaatca taatcaagga aattacccaa tgagatctgt gaaaacaaca
     2941 atagtagtga tggttatgag cttattcttc ggtagctcaa attgttttgc aatggataca
     3001 gttgatccgg aattgctttc cgccatggaa taccgactgg taggaccaca tcgaggtggc
     3061 cgtgttacaa cagttgcagg ggtgccagac aaaccacaac tatattacat gggagcaact
     3121 ggtggtggtg tgtggaagac agaaaatgct ggtttgactt gggaaaatat ctcggatggt
     3181 ttttttaacg tcggcaccat tggtgccatt gctgtggcag aatctgatca taatgttatc
     3241 tatgttggta ccggagaaaa gtctatccgt ggtgtgacta ctagtcaggg agacggtatc
     3301 tataaatcaa cggatgcagg tgcaacctgg atgcatatcg gactccctaa agcaggacaa
     3361 atttcacgta tcaagattca tccaacgaat cctgatattg cctatgccgg ggcacagggt
     3421 cagatctggg ggcccaataa agagcgtggt gtctatcgta cgatggatgg tggcaagact
     3481 tgggagcagg ttttgagtgt tgatgcacaa actggtgcga ctgatcttag aatggatccg
     3541 accaatccaa ggattctgta tgcagccatg tgggagcatg gccgcaaacc ttggtatgtc
     3601 ttatctggtg gcactgccgg ggggattttt aagtcgacag atgcgggtga taactgggag
     3661 aagctaacca atggtctgcc tgaactgatt ggcaagatcg gagtggatgt ttctgcaagt
     3721 aacccgaatc gattgtacgc aattgttgaa gctatgcctg gtgagggcgg cttgtaccgt
     3781 agtgatgatg gtgggacctc ttgggaattg aaaaacaagt cacgtattct ttggacccgc
     3841 tcctggtact atatgcacat cgcggctgac ccagtgaacg aggatattgt ctacgtactc
     3901 aatgcaccat ttatgaaatc aattgatggc ggtgtgactt ttgaaaagaa atccacacca
     3961 catggtgatc atcatgatca ctggattaat ccctataaca ataagaacat gatcaacggt
     4021 aatgatggtg gtgcgactat tacttttgat ggtggtgaat cctggtcgag tattatgaac
     4081 cagccaacag cacagtttta ccgaatcaat acagataatc agttcccgta ccgtatttat
     4141 gcagggcaac aagacaacag tactgtcgcc attcttagtc aaacgtatgg caacagtata
     4201 ggtaatgatg acttttatcc ggtagggggt ggcgagagtg ctcatattgc tttcgatcct
     4261 gacaatccac gcctgatcta tgccactagt atcaacagta cactcactga atatgatgct
     4321 gataatgaac gcattagaga aattaaacct tatccagaat ttatatttgg aatggagtca
     4381 aaggatctgc gctaccgtac caactggaat gctcccgtta ctgcatcacc ccaggatccc
     4441 agcgttatct attatggtac agaaaagcta ttgaagacca ctgaccgcgg agtgacattt
     4501 gaacaaatca gtccggattt gaccaagaat gaaaaagata agcaaggcct gaatggtgga
     4561 cctattactg ccgagaatgt cggtgcagaa ttttatggta ctattctgac catcaccgca
     4621 tcgccacatg aatatggcac ggtttgggtt ggtagtgacg atggtctaat ccatgtcact
     4681 cgtaatgatg gcgaaagctg gaacgaagtc acacctcaag atctacgtgg tgccatgatt
     4741 aactctatcg aagtgagtcc gcacgatccc ggcacggctt atgttgctgt cgctggctat
     4801 aagatgaatg atttcaggcc atacatctat aaactgacgg attttggacg acaatcgaga
     4861 agaattgata gggggttacc agatgataat tttattcgtg tcgttcgtga agatcctgaa
     4921 cggcagggac tcctattcgc tggtagtgaa gttagtctct atgtgtcttt tgatgatggt
     4981 gataactggc agtcactggc tctcaacctg ccgccggtac caattactga tctagcaatt
     5041 cgtcaaaatg accttgttgt ctccacgcaa ggcagggccc tctgggtgct tgacgacctg
     5101 tctgctctgc gtcaagtgca aaatgacatg ataagtaagc cactgcatgt ttttacccct
     5161 ggaccagttg agatgattaa gggtagtaat ccatctgaac cctccaagac caagggcacg
     5221 aacccccttc gtggcgttgt attcaactac cacattcgtg atgaacaaga agatccgtta
     5281 accattgaga tcaccgataa taacggtaat cacgtgcgca cctattcaag tgaagaaagt
     5341 gactttgaac gttgtatcat tgctaatata gatcagcgtc taccgtttaa agtcaaatat
     5401 ccaccaaaag agcagggtgc taacaagtgg gtctgggata tgcgcagaca tggtctgcat
     5461 tgcattgatg atattaagtt attcgaggga tttgctggcg catatgttat gcctggcacc
     5521 tatcaggcac gtatttcaat tggtggtgct gaagatactg tgaccctcaa tctcatcaca
     5581 gatcgtcgaa ttacagctga cagtcacgct attgccgaag ttgaacaaaa ggttttcgat
     5641 ttaacagcgt tgatgaacga gttgcttgac agtctaacct ctattcgaaa gtcaagaact
     5701 cagatcaaga ctttgatgac tgacttccct gacgcagagt tgcttcagga ttctggtcag
     5761 agtgcaattg atcgtctatc tgcatgggaa agagaagttc tgcaggttga ttacgagact
     5821 tacgaagatg aagataatct tccaggcaag cttgtcaaac aggtgcgtca tctactggat
     5881 gtaatcgacg atgcaggtcc accaatcgca gcaggtgcgc ttgaacgatt gagagacctt
     5941 gaagatgaat gggccaattt acaggctgaa ctacgagaca tttcaacctc agatatcgag
     6001 gctgtcaata attgggccag tacaaatact atcccacata tttcaccacc cattgcacgg
     6061 cgctaatttt ggcacgtaga tgaaattcca tggttcattt tccaatgtga taagcgttta
     6121 ggtatgcagc aaagatcaaa gaaaatccct atcattatta tcattgtgtg cattcttatt
     6181 gtagtgcttt caatctactt agaaggtaat aaaaaagctg aaaaaattgg acctgatttg
     6241 attgttttca atgctgatat cagaacctca gacccaaagt ctccaatagc tcaagctttt
     6301 gctgtcaaca aaggaaaatt tattgctata ggaaccaatc aagagattca gagcttagga
     6361 aaaaaaaata cacaacaaat tgatgcacaa gggtttagcg ttttgcctgg gttcatagac
     6421 agccataccc acttatcctc gggtggaaaa attgttaccg gggttaattt aactggtgtt
     6481 aaagaaaaat ctgtgtggtt agatatgatt gcagaaaaag tcaagacaat ggagccgggt
     6541 gaatggttat tgggtggtcg ctgggattac actcttgaaa ataagggctt acctacccgt
     6601 tgggaattag acgaagtttc tccacataac ccagtggcac tatctgatat tgatggccat
     6661 agcatgtggg taaatacatt agctatcgag aaggccaaga tcacaactga ttcaattgtt
     6721 ccagtcggtg gacaaataat gct
//
LOCUS       DASCTH010000158         4681 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000225986, whole genome shotgun sequence.
ACCESSION   DASCTH010000158 DASCTH010000000
VERSION     DASCTH010000158.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000225986"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(227..2281)
                     /gene="fusA"
                     /locus_tag="QGH35_03375"
     CDS             complement(227..2281)
                     /gene="fusA"
                     /locus_tag="QGH35_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HJL81608.1"
                     /translation="MTHQTLHSTKNIRNIAVVGHAGSGKTTLIEALLEKSGAIQSAGS
                     VDKGTTVCDFTDQEKRLQHSLDVSTCYLETDGTFINLLDTPGYPDFMGRALSVLPAVD
                     TAAIVVNAQTGIELITQRMMDFSAQRKLCRLIIINKIDAENINLQDIFKEIRTTFGRQ
                     CLPLNLPAQQGKAIADCFFESTDETPDFASVEAAHTEIVDQVVELDDELMEAYLEQDE
                     DINPDQLHDPFERALRAGHLIPVCFVSSDTGVGIQQLLRILSKLMPSPMEGNPPEFFN
                     DQGEKKAIIMSAGDMDGHFVGHVFKVNVDPYIGRLATFRVHQGSISKGDQVFIGDQRK
                     TVRLAHLYQAQGKDLKEISTAVSGDLCSVSKIEDLRFDTVVHSSHDEDELHMKSMPMP
                     PPMYGVALELMKRGQEKKLSDALNKLTTEDPSVKVEFNAQVNETVLRGMGELHLRVAL
                     ECMRKEFGINISTHPPKIAYLETITCQAEGHHRHKKQTGGAGQFGEVFLRIEPLPRGS
                     GFEFSNKVVGGNIPRQFIPAVEKGVKQVLDEGAIAGYPLQDVKVIVYDGKHHPVDSKE
                     VAFVAAGKRAFSDAISKAKPMVLEPIAKIEVTIPSSYVGDITGHLSGIRGRIAGSVAI
                     AGNRVRISAEVPLAELGDYQTTLKSLTGGEGTFAMELDHYEKTPPAAQKDLEKAFRPD
                     IS"
     gene            complement(2329..2685)
                     /gene="erpA"
                     /locus_tag="QGH35_03380"
     CDS             complement(2329..2685)
                     /gene="erpA"
                     /locus_tag="QGH35_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HJL81609.1"
                     /translation="MSVKADNNIGTEAIIFTDAAAMKVGSLLSEEENPNLNLRVYVTG
                     GGCAGFQYGFSFDESKEEDDTEVIKRGVRLVVDPMSIQYLTGAEIDFQEDLQGARFVI
                     KNPNAATTCSCGSSFS"
     gene            complement(2725..3213)
                     /locus_tag="QGH35_03385"
     CDS             complement(2725..3213)
                     /locus_tag="QGH35_03385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010560756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HJL81610.1"
                     /translation="MTDVIDQQGYRANVGIVLINNRQELFFAKRKHQRGWQFPQGGIR
                     IGESLTSAMYRELLEETGLTKDHVEVIYQSKEWYQYKIPKNFLKKLNNASPQVVGQKQ
                     KWFLVKLNGSDELVDLTCDSKKNQEFDEWKWVNPSIPAKEVVWFKQDVYEKVLNEFLT
                     FL"
     gene            complement(3219..4307)
                     /locus_tag="QGH35_03390"
     CDS             complement(3219..4307)
                     /locus_tag="QGH35_03390"
                     /inference="COORDINATES: protein motif:HMM:NF042869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HJL81611.1"
                     /translation="MTDLKVLREQLTDVDMQIIDLIHQRQSLSIKIGSIKRKAGQPTQ
                     DFLREKQVLELAADYAEKLNMQPDVIVDVMQLLIKSSLRTQEKARVQEEGQGSGQSAL
                     VIGGAGRMGNWFVEFLKSQGFEVFIADPKAECDEVKTFASWKSLEDNFDITVVAAPLR
                     ESLTILNEILEEGRSGLIFDIGSLKTPLKKTLWQMSKKGIKVTSIHPMFGPDTDLLTG
                     KHIIFMNVGSTESLQEAQKLFEATTAQQIEMSLENHDYAISYVLGLSHVLNIAFAKAL
                     YSSGENKDLLTNLSSTTFKDQLDVAKRVTDENPHLYYEIQYLNEFSLKTISELSNAIK
                     EIFDFISSGDEDKFVQLMEQGRSYFSEK"
     gene            4435..4671
                     /locus_tag="QGH35_03395"
     CDS             4435..4671
                     /locus_tag="QGH35_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJL81612.1"
                     /translation="MSKIINQSSIKNPLTVLKSWLQEAIESELQPNPNSMALATVNSK
                     GEPNVRMLLCNEINIEQWYIVFFTNYNSVKGFEI"
BASE COUNT         1299 a         1014 c          868 g         1500 t
ORIGIN      
        1 aatcaatata aagctgatac ggaaaaactt aaaaagcctt tggtatgttt ttgtcatagt
       61 tgcctcgatt taattttaat ggatgagttt aatctttacg gatcagcgat tgtcttttaa
      121 tagcagatag tgaggtcgcg cctacaaact tcatgatagt ctccagttct gtattacgtg
      181 caaggattct tggatttgat atccctccct tatctgtttg cattctctat gagatgtcag
      241 gtcggaaagc tttttccaaa tctttttgtg cagccggcgg ggttttttca tagtgatcaa
      301 gttccatagc gaaagtgccc tcacctcctg tgagtgattt gagtgtggtc tggtagtctc
      361 cgagctccgc caatggcact tctgctgata ttctcaccct atttcctgca attgcgacac
      421 taccagcgat tcgacctcga atgccagaca aatggccagt gatatcgccc acatagctac
      481 tagggatagt gacctctatt ttagcaattg gctccagcac catgggtttt gctttggaaa
      541 tagcgtcaga gaaagcacgt ttacccgcgg cgacgaatgc aacttccttg gaatccaccg
      601 ggtggtgttt accatcgtag acaatgacct tcacgtcctg caacggataa ccagcaatag
      661 caccttcgtc gagcacttgt ttaacaccct tctccactgc aggtatgaac tgcctcggta
      721 tattgccacc aacaacttta ttggaaaatt caaacccact acctcgtggt aatggttcaa
      781 ttctcaagaa tacttctcca aactgtcctg caccgcctgt ctgcttttta tgtcgatggt
      841 gaccctcagc ttgacaggtt atagtctcca agtaagctat cttgggtggg tgagtggaga
      901 tattgatgcc gaactctttc ctcatacact caagcgcaac acggagatgt agctcaccca
      961 tccctcgaag cacggtctca tttacctgag cattgaattc taccttcacg cttggatcct
     1021 ctgtggtcag cttattcaat gcgtctgaca actttttttc ctgaccccgt ttcatgagtt
     1081 ccaaggccac gccatacatg ggtggtggca ttggcataga cttcatgtgc agttcatctt
     1141 catcgtgact ggaatgaaca acagtatcga aacgaaggtc ttctattttt gagacagaac
     1201 aaagatcccc tgatactgcg gttgaaattt ctttaagatc tttaccctgg gcttgataca
     1261 aatgagcaag tcttactgtt tttcgttgat caccgataaa aacctgatca cccttgctaa
     1321 ttgaaccttg atggactcga aaggtcgcga gtcgacctat ataaggatca acgttgacct
     1381 tgaagacatg gcctacaaaa tggccatcca tgtcgcccgc actcattatg atagcttttt
     1441 tttccccttg atcattaaag aattccggag gatttccctc catcggactt ggcatgagct
     1501 tggataaaat acgtaacaat tgttgaatac cgacacctgt atcagatgaa acgaagcaga
     1561 caggaattaa gtggccggcc cgcaaggcac gctcaaacgg atcatggagc tgatccggat
     1621 tgatgtcctc atcctgctcc agataggctt ccataagttc atcatctagc tccacgactt
     1681 ggtcaacgat ttccgtatgg gctgcttcca cagaagcgaa gtctggtgtt tcatctgtgg
     1741 attcaaagaa acaatctgct atggcttttc cttgttgcgc aggtagattt agtggcaggc
     1801 attgtcggcc aaatgttgtt ctaatttctt taaatatgtc ttgtaggttt atattttctg
     1861 catcgatttt gttaataatg ataagccgac agagtttgcg ttgtgcactg aagtccatca
     1921 tgcgttgagt aataagttcg atacctgttt gggcattgac aacaatcgcc gcggtatcga
     1981 cagcgggtaa cacactcaag gcgcgtccca tgaaatccgg atatccagga gtgtcgagca
     2041 gattgatgaa agtgccatcg gtttcaagat aacatgtgct cacatcgagt gagtgttgga
     2101 ggcgcttctc ttggtctgtg aaatcacaaa ctgtggtacc cttgtcgaca ctcccggctg
     2161 attgaatagc tcctgacttt tctagcaggg cttcgatcag tgtggttttt ccggaccctg
     2221 catggcctac cacagcaata tttcgaatat tcttagtgct atggagtgtc tgatgtgtca
     2281 ccgctgaaat ccctccttgc tacttaaaag aaaatattaa tcttctgatt atgaaaaaga
     2341 agaaccacaa ctgcatgtag ttgcagcatt aggatttttt atgacaaacc tagcaccttg
     2401 aagatcttct tgaaagtcaa tttcggcccc ggttaaatac tgaatactca ttggatctac
     2461 cacaagtctc acccctctct ttatcacctc agtatcatcc tcctctttgc tttcatcaaa
     2521 ggaaaatccg tactgaaaac cggcacaacc acctccggtt acatacactc ttaaatttaa
     2581 attcgggttt tcctcttcag atagtaacga ccctactttc atggcagcag catccgtaaa
     2641 tataatagct tcggtgccta tgttgttgtc tgcttttact gacatagtat ccatgttatc
     2701 tgattgcagt tgtaaaatga actttcaaag aaaagttagg aattcgttca aaaccttttc
     2761 atagacgtct tgtttgaacc aaaccacttc ttttgctggg attgaaggat taacccattt
     2821 ccactcatca aattcttgat ttttttttga atcacaggtc aagtccacta attcatctga
     2881 gccattgagt ttgactaaaa accatttctg tttctgacca acgacctgag gtgaagcatt
     2941 gttcagcttt tttaaaaaat ttttaggaat tttatattga taccattctt tagattgata
     3001 gatgacttca acatggtctt ttgttagacc tgtttcttcc aacagctctc gatacattgc
     3061 agatgtcaga gattctccaa tcctgatacc tccttgtgga aattgccaac cacgctggtg
     3121 tttccttttt gcaaaaaata gttcttgccg attattaatt aaaactatac caacattagc
     3181 cctatagccc tgttgatcaa ttacatcagt cataatagtt atttctctga gaaataagat
     3241 ctaccctgtt ccatcaattg aacaaattta tcttcatctc cagaacttat aaaatcaaaa
     3301 atttctttaa tagcgttact gagttctgag attgttttta gactaaattc attcaaatat
     3361 tgaatttcat aatacagatg agggttttca tcagttactc ttttcgcaac atctagttgg
     3421 tctttaaatg tggtactaga caaattagtg agcaaatcct tattctctcc actagaataa
     3481 agggcttttg caaaagctat attaagcaca tgtgataacc cgagaacgta tgaaatggca
     3541 taatcatgat tctcaagtga catttctatt tgttgagcag ttgttgcttc aaaaagtttt
     3601 tgtgcttctt gcaatgactc cgttgaacca acattcataa agatgatgtg tttaccagtc
     3661 agtaaatctg tatctgggcc aaacatcgga tgtatagatg ttaccttgat gcctttttta
     3721 gacatttgcc ataaggtctt tttcaacggt gtcttcaaag atccaatatc aaaaataagt
     3781 ccacttctac cttcttcaag gatttcattt aaaattgtta atgattctct gagaggtgct
     3841 gctacaacag tgatatcgaa attatcttcc agtgacttcc atgatgcaaa agttttaact
     3901 tcatcacatt cggctttggg atctgctata aatacctcaa aaccttgaga tttcaaaaat
     3961 tctacaaacc aattacccat tctacctgcg ccaccgataa ccaatgccga ttgaccactg
     4021 ccttgtcctt cttcctggac ccttgctttc tcctgagttc gcaatgagct cttaattaaa
     4081 agttgcatta catcaacgat aacatcgggt tgcatattca atttttctgc ataatctgca
     4141 gctaattcta agacctgttt ttctcgtaaa aaatcttgag ttggttgacc tgcttttctt
     4201 ttgattgagc caatctttat agacaatgac tggcgttgat gaataagatc aatgatctgc
     4261 atgtccacat cagtgagttg ttctctgagt actttaagat cagtcataca ttacagtcct
     4321 tcattggtag ttattcaaaa agttataata aattacgaag aattttttcg ctggctcctt
     4381 aaggctggta tgattcttag tggagactta ttatatcaaa tcgattatta cattatgtct
     4441 aagataataa atcaatcttc aataaaaaac ccacttacag ttttaaaatc atggctgcag
     4501 gaagcaattg aatcggaatt gcaacccaac ccaaattcaa tggcattggc tactgtcaat
     4561 tccaaaggag aaccaaatgt caggatgctc ttatgtaatg aaataaatat cgaacaatgg
     4621 tatatagttt tttttactaa ttataattct gttaaaggct ttgagattta agataactca
     4681 a
//
LOCUS       DASCTH010000159         5651 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000227129, whole genome shotgun sequence.
ACCESSION   DASCTH010000159 DASCTH010000000
VERSION     DASCTH010000159.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000227129"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="QGH35_03400"
     CDS             <1..1115
                     /locus_tag="QGH35_03400"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HJL81613.1"
                     /translation="GGGGSGVVVDGTKGYIVTNHHVIEKADEIKVFLQDGSEYLAELV
                     GSDPGTDIAILKIENPKKIAEIPLGNSNLLRVGDFALAIGAPFGLSQTVTSGIISALG
                     RPQMSSDGYGDYIQTDAAINPGNSGGALINLEGKLIGINSAIYTRSGGNIGIGFAIPV
                     NTVKNIMNQLIEFGSVKRGLLGVIISDINKDIADELGLEIIKGALIQEVSPNSAAEEA
                     GLLVGDVIFRVDDQEINKSNELRNAIGLKRSGESVALTILRDNKEISVNAILGELMAM
                     EPVKADELNSLLAGAQLSDYIPEGETETKGVVILSIEPNSNAARARLKSGDIIWAVGN
                     TTIGNLEDFIAIIKDKDILVLRVSRNGRQLIIQMRK"
     gene            1152..2282
                     /locus_tag="QGH35_03405"
     CDS             1152..2282
                     /locus_tag="QGH35_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013871.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HJL81614.1"
                     /translation="MEIYLVGGAIRDRLLDLPSGEKDWVVVHATPSEMKHLGYKQVGK
                     SFPVFIHPETGEEYALARKEVKTGTGYHGFDFDANPDITLEEDLSRRDLTINAIAENK
                     DGEIIDPFKGREDLSKRVLRHVSEAFKEDPLRVLRIARFRAKLFNLDFNIADETKQLV
                     AKIVASGELSSLVPERVWMETKKALNESRFDQYFKTLIEFGAFDQIYPDLSGIDKDFF
                     SQSIFQEASQANISSRYRFAAIFLALMEQEQESIKDRVESMQNNMNLPNKFKELPLQI
                     DQSMSLISKDIEQYNAEYILTLLETLDVVRRPDHLEDVIAVITLGQGQGLDEKIALLQ
                     KSADLARSIKISNLREDGLDGNVIGKKIRELRLKKIEEELAK"
     gene            complement(2321..3103)
                     /locus_tag="QGH35_03410"
     CDS             complement(2321..3103)
                     /locus_tag="QGH35_03410"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020027702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HJL81615.1"
                     /translation="METLQAILLAIIQGLTEFLPISSSAHLILLSELSGWQDQGLVFD
                     VGLHFGTLLAVIFYFREDLLNMFCPAHFKSIDALIHSQLGIILVATIPVVLVGGLFNQ
                     LIEQHLRSPMIIAMATAFFGLLLFLADYRNKHKGKGIAVDLGIGFLIGLSQVFSLIPG
                     TSRSGITITAGLFLGLSREEAAKFSFLLAIPVIIAASVFELYQLSQVEALIFDYLDLF
                     LGIIVSFVVAFLTIKWFLNMVQKIGMLPFFVYRILLAVFLLV"
     gene            complement(3112..4287)
                     /locus_tag="QGH35_03415"
     CDS             complement(3112..4287)
                     /locus_tag="QGH35_03415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HJL81616.1"
                     /translation="MQSIKQQLPTPDDASLRHSSVLVEKLTERIKQKKGWISFADYMQ
                     FVLYEPGLGYYSAGTQKLGKGGDFTTAPEISDLFGISIADYLITALDGLKSPSLLEIG
                     AGSGQLALNILSQLETEAFELEQYYILELSADLKERQQQLLSQLPGVAAQVTWLDSLP
                     DNLITGAIIGNEVIDAIPFQRFKIAGNDIYELGVSSGSNQLYEQEQQPSEALTESVRL
                     IEQKLGYQLPDQYQSEVRLNYEDWFRSVSAALTQGIMIFIDYGLCRSEYYSPSRSDGT
                     LVCHYQNRAHYEPLLFPGLQDISAWVDFSLVADNALNQGCEVFGYTSQMEFLLSSGIL
                     GRMDAKHPQDEEYNINQSLKQLLLPSQMGEAFKCMLLSKNYNLGMDNLEAKDYRYML"
     gene            4360..5103
                     /locus_tag="QGH35_03420"
     CDS             4360..5103
                     /locus_tag="QGH35_03420"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006035378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HJL81617.1"
                     /translation="MNDTNNKWALITGGSKRIGAKMAETLHDAGINIAIHFNSSSDSA
                     TELCAQLNAKRADSSITLKADLTNQEALEHLVPSLIEQTHRLDVLINNASTFYPTPIQ
                     SITLTDWDDLMGTNLKAPLFLCKYAAEHLIKSSGVILNIIDIHAKKPLKDHSIYGSAK
                     AGLATLTRSLAKDLAPDVRVNGISPGLILWPEDEPPEDIKNNILEQIPLKNPGTPEDI
                     AQCALFFIQDAPYATGQIIAVDGGRSIGW"
     gene            complement(5104..5619)
                     /gene="folK"
                     /locus_tag="QGH35_03425"
     CDS             complement(5104..5619)
                     /gene="folK"
                     /locus_tag="QGH35_03425"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HJL81618.1"
                     /translation="MTDTDQPKPVDVFLSIGSNVNPEVNIQYALSEIDQIFEIKQVSS
                     VYKNKSVGFEGDDFLNMVLKLSTTLSPYEIVEELNRIELATGREIGTGTFDSRTLDID
                     MILYGDLVDPEEPLQLPRKDIDLYSFVIGPLAEIEPRGIHPVLGKSFIELWEEFNQEA
                     YPLERTSIRFD"
BASE COUNT         1844 a         1093 c         1114 g         1600 t
ORIGIN      
        1 ttggtggagg aggatctggt gtagtggttg atggaacaaa aggatacatc gtcaccaatc
       61 atcatgtcat tgaaaaagca gatgagataa aagtttttct acaagatggt agtgaatatt
      121 tggcagagtt agtgggttcg gacccaggta cggatatagc catactaaaa atagaaaacc
      181 caaaaaaaat agctgaaata ccattgggca actctaatct tttacgagtt ggagattttg
      241 cactcgctat tggagcacca tttggtctga gtcaaaccgt cacttctgga ataatcagtg
      301 cactgggaag acctcaaatg agttctgatg gctatggaga ttatattcaa actgatgcgg
      361 ccattaatcc aggtaattca ggcggtgctt taattaattt agaaggaaaa cttataggga
      421 tcaatagtgc tatctatacc agaagtggtg gcaacatagg aattggcttt gcaattcctg
      481 tcaacacagt caaaaatata atgaatcaac tgattgaatt tggttcagtc aaaaggggtt
      541 tgttgggagt cattattagt gatatcaaca aagatattgc cgatgagctt gggcttgaaa
      601 tcatcaaagg ggcattaatt caagaagttt cacctaattc agctgcagag gaagctgggc
      661 ttttggtggg tgatgtcata tttagggttg acgaccaaga aataaataaa tctaatgagt
      721 tacgcaatgc tattgggctc aaaagaagtg gtgaatctgt tgcactgacc atccttagag
      781 acaacaagga gattagtgta aacgctattc taggagaatt gatggcgatg gaacctgtaa
      841 aagcagatga actaaattct ttgctagcag gagctcagtt atcagattac attccagagg
      901 gagaaactga gacaaaagga gttgtgattc tttctattga gcccaacagt aatgctgcca
      961 gagctagatt gaaaagtggg gatattatct gggctgttgg taataccact attggtaacc
     1021 tagaagactt tatcgcaatt attaaagata aagatattct ggtcttgaga gtcagcagaa
     1081 atggtcgtca attgatcata caaatgagaa aataaattga atcattattt catatttatt
     1141 ttttagacag catggaaatt tatttggttg gaggagcaat tcgagatcgg ctcctcgatc
     1201 taccttcagg tgagaaagac tgggtggtgg tccatgcaac cccctcagaa atgaaacacc
     1261 tcggttacaa acaggttgga aagtcatttc ctgtctttat ccacccagaa acaggcgaag
     1321 aatatgcctt ggctagaaaa gaagtcaaga caggtacggg ttatcatgga tttgattttg
     1381 atgccaatcc tgatattaca ttggaagaag acctctctag aagagacctg acaatcaatg
     1441 ccatagctga gaataaagat ggtgagatca tagatccgtt taagggcaga gaagatctgt
     1501 ctaaaagagt cctccgccat gtatctgagg cattcaaaga agaccctctc agagtgcttc
     1561 gtattgcaag atttagagct aaactgttta acttagactt taacatcgca gatgaaacca
     1621 aacaactagt ggccaagata gtcgcaagcg gtgagctatc ctctcttgta cccgaaagag
     1681 tgtggatgga aactaaaaaa gccttaaatg aatccagatt tgatcaatat ttcaaaacac
     1741 tcattgaatt cggtgccttt gatcaaattt atccagatct atctggaatc gataaagact
     1801 tcttctcaca atctattttt caagaggctt ctcaggcaaa catatcatca aggtatagat
     1861 ttgctgctat tttcttagcg ttgatggaac aggaacaaga aagcattaaa gatagagttg
     1921 aatcgatgca gaataatatg aatttaccaa ataaattcaa agaattgcca ttgcagatag
     1981 atcagtctat gtctttaatt tcaaaggata tagaacaata caacgctgaa tatattctta
     2041 cactattaga aacacttgat gtggtacgca gacctgatca cttggaagat gtcatcgcag
     2101 taataacact gggccaaggt cagggtttgg atgaaaaaat agcactctta caaaagtctg
     2161 cagatcttgc tcgttcaatc aaaattagta atctccgtga agatggtctt gatgggaatg
     2221 tcattgggaa gaaaataaga gaactaagat taaaaaagat tgaagaagaa ttagcgaagt
     2281 aaattgactc ttgaactccg gagtgacaat cgtttgaagc tcataccagc aagaatactg
     2341 ccaataaaat tcgataaaca aaaaatggca gcatcccaat cttttgaacc atgtttaaga
     2401 accatttgat ggttaaaaat gccacaacaa aagacacaat aattcctaag aacaaatcca
     2461 aatagtcaaa aatgagagcc tcgacttggc ttaattgata gagctcaaat acacttgcag
     2521 cgataatgac tggaattgct agtaaaaaag aaaattttgc agcctcctct cttgataaac
     2581 ccaggaaaag tccagctgtg atcgtgatcc cagatcttga tgtgcctggg attaatgaaa
     2641 atacctgaga taagcctatt aaaaacccta ttcctaaatc aactgcaatg cctttaccct
     2701 tatgtttatt cctataatcg gctaaaaaca atagcagacc aaagaaagcc gttgccatgg
     2761 caataatcat cggagatctc aagtgctgct ctatcaactg gttgaacaat ccaccaacta
     2821 acaccactgg gatagtagcc actaatatga tgcccaattg ggaatgaata agagcatcaa
     2881 tacttttaaa atgagcagga cagaacatat tcaacaaatc ctctctaaaa taaaaaatga
     2941 cagctaacag tgttccaaaa tgcagaccca catcaaaaac caatccctgg tcttgccatc
     3001 ctgaaagctc acttaataaa atcaagtgag cagaactgga aatgggaaga aactcagtta
     3061 gcccttggat gattgctaat aaaattgctt gtagtgtttc catatgtgag attaaagcat
     3121 atagcggtaa tctttagctt caagattatc cataccaagg ttgtagttct tgcttaacag
     3181 catacattta aaagcttccc ccatttgact gggaagaagc aattgtttca atgattgatt
     3241 aatattatat tcctcatctt gtggatgttt tgcatccatt cgaccaagaa taccagagga
     3301 taataaaaac tccatttggg aagtatatcc aaacacttcg caaccttggt tcagagcatt
     3361 gtcagcaacc aagctaaaat ctacccatgc agaaatatcc tgtaggcctg gaaacaacag
     3421 aggttcataa tgggccctgt tttggtaatg acacactaag gtgccatctg atcttgatgg
     3481 tgaataatat tcgcttctgc agagtccata atcgataaag atcatgatcc cttgggttaa
     3541 agcagccgat actgacctaa accagtcctc ataattcagt ctgacttctg actgatattg
     3601 atccggcaac tgatagccca gtttctgttc aatcaatctt actgattcag ttagagcctc
     3661 tgaaggctgt tgttcttgct catagagttg atttgatcca gacgacaccc ccaactcata
     3721 aatatcattg cctgcaatct tgaatctctg gaaggggatc gcatcaatga cttcattgcc
     3781 aataatggcc ccagtaatca aattatctgg cagggaatcc aaccaagtta cttgagccgc
     3841 cacccctggc aattgtgata atagttgttg ctgcctttct tttaaatcag cactgagttc
     3901 gagtatgtag tattgctcca gttcaaatgc ttcagtttcc aactgcgaca aaatattcaa
     3961 tgccaattgt ccagatccag cgcctatttc cagtaaactt ggtgatttca acccatcgag
     4021 agcagttatg aggtaatctg caattgagat tccaaataga tcagagatct caggtgcggt
     4081 ggtaaaatca ccccccttgc ctaacttttg ggtacctgca gaatagtatc ccaatccagg
     4141 ttcatacaaa acaaactgca tgtagtcagc aaaactaatc cagcctttct tttgtttaat
     4201 tctctcagta agtttttcaa ccagaacaga agaatgccga agtgaggcgt cgtctggtgt
     4261 tggtaattgt tgtttaatag attgcaaaat acttacctct ttcagagtat tatctagaat
     4321 taataacgct aatgttagtt ttcaattaaa ataacgatta tgaatgatac aaacaataaa
     4381 tgggcactga tcactggcgg tagtaaacgc attggagcca aaatggcaga aaccttgcat
     4441 gatgcaggaa ttaatatagc cattcatttt aactcttcaa gtgattcagc tacagagttg
     4501 tgcgctcaac ttaatgccaa gagagctgat tcctcgataa cattaaaggc tgatttaact
     4561 aatcaagagg ccttggaaca tttagttcct tccctaattg agcaaaccca cagattggat
     4621 gtcttgatta acaatgcatc cactttctac cctacaccca tacaatcaat caccctaaca
     4681 gactgggatg acctaatggg aaccaactta aaagcacctt tatttttgtg taaatatgct
     4741 gctgaacact taataaaatc aagtggagtt atcctcaata tcattgatat ccatgctaaa
     4801 aaacctttaa aagatcattc aatttatgga tctgcaaaag caggtctggc tacgctgaca
     4861 cgatcactgg caaaagattt ggccccggat gtcagggtta atggtatttc accgggttta
     4921 atactatggc ctgaggatga acctccggaa gatataaaaa ataatatact ggagcaaatt
     4981 cctcttaaaa accctggcac acctgaagac atcgctcagt gtgctttgtt cttcatacaa
     5041 gatgctccct atgccaccgg acagattatt gcagttgatg gcggcagaag catcggctgg
     5101 taactagtca aatctgattg aggttctctc taagggatat gcctcttgat taaattcttc
     5161 ccataattcg atgaagctct ttcctaaaac ggggtgaatg cctctgggct caatttctgc
     5221 tagagggcct atgacaaaac tgtatagatc gatgtctttt cgcggaagtt gcaaaggttc
     5281 ttctgggtcc actagatcgc catagaggat catatcaata tcaagtgtcc tggagtcaaa
     5341 agtgcctgta ccaatttctc ttcccgttgc caactctatt ctattgagtt cttccactat
     5401 ctcataagga ctaagggttg tgcttaattt caaaaccata tttaaaaaat catcgccctc
     5461 aaatccaacg gatttatttt tgtaaacaga agatacttgt ttgatctcaa atatttgatc
     5521 tatttctgat aatgcatatt gaatatttac ctcgggattc acattgctgc ctatactgag
     5581 aaatacatca accggtttgg gttgatctgt atcagtcatg tttactgcgt tctatacaaa
     5641 tcccaacgtt c
//
LOCUS       DASCTH010000160         2493 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000233007, whole genome shotgun sequence.
ACCESSION   DASCTH010000160 DASCTH010000000
VERSION     DASCTH010000160.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000233007"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="QGH35_03430"
     CDS             <1..1544
                     /locus_tag="QGH35_03430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81619.1"
                     /translation="GAKKLVDENTQTINLNGKMILPGFHDIHVHPVHSGVLYQQCTLF
                     DILGVEKLLSKVKECALANPEDEWIIGGGWTVPDFAPSGLPDKKLLDEIVSDRPVSLK
                     SSDGHSLWVNSKALELAGITAATPDPENGRIDRYPNSQEPSGSLQEDSAIMLVMDLEP
                     ELTTEDLINGLKYSRDLFHSLGITGVQDAILKLKPGDAYYGLDAYNYLDNRNELNLHV
                     VTALFWENSKPLDQQLPQFLKIRQQQQTNGNVKATSIKIWQDGVIETQTAALLEPYSD
                     RSDGYRGDLQNTPDNLNTAVVALDAAGFQIHFHAIGDRAIRVSLDALEEAINSNGTRD
                     SRHHLSHIELFNPADIPRFAEMNVIANFQPLWAIQDEYITDLTWPRLGEERSKWLYPI
                     GSMQRTGAKVGFGSDWYVTSVNPLDGIETAVTRLDPNGLTDVPLGQNEEISLAQAIEN
                     YTLNSAYVNFLDHRVGSVEVGKQADLVILDRNLFAIPTSEINEAKVIATLFEGRLVYG
                     TLKIQ"
     gene            complement(1565..2245)
                     /locus_tag="QGH35_03435"
     CDS             complement(1565..2245)
                     /locus_tag="QGH35_03435"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJL81620.1"
                     /translation="MIYNPKNRRDGTIEKVHDKDNFYKIYDWAGSDRSKLPWAHDEPT
                     LFLRDIVGQRGKPGKALDIGCGAGTDSLYLAGENWDVTSLDFMPAALEMTCLRATAAG
                     LKLTTIEADVTEWQPTDKFDLVLDHGLLHNMNPDRYPLYRERVMQALADDGEFLILHW
                     LQRTPDEPQSIIGPTRVSREDIQKFFAPELKERFFAIEEIDGQNVSVGVSIAQGYYWF
                     KRTSTGSA"
BASE COUNT          726 a          510 c          565 g          692 t
ORIGIN      
        1 aaggtgccaa aaaattggtt gacgagaaca cccaaacaat aaacctcaat ggaaaaatga
       61 tactgcccgg ttttcacgat atacatgtgc atcctgttca tagtggagtg ctttaccagc
      121 aatgcaccct gtttgacatt ctgggagtag agaagcttct gagtaaggta aaagaatgtg
      181 ctcttgccaa ccctgaagat gaatggatca taggaggcgg atggacagta cctgattttg
      241 ctccctcagg tttgcctgat aaaaaattat tggatgaaat agtttcagat cgaccggttt
      301 cactcaaatc atcggatggg cattcactct gggttaattc caaagcttta gaactggccg
      361 gtataactgc agcaacacct gatccagaga atggcaggat tgatcgatat cccaactcac
      421 aggaaccctc gggcagttta caagaagaca gtgccatcat gttggttatg gatcttgagc
      481 cggaattgac tacagaagat ctcatcaatg ggcttaagta ttcccgtgat ttatttcata
      541 gtttagggat tactggtgtc caggatgcaa tattaaagct taaacctggt gatgcgtact
      601 atggactaga tgcatacaac tacctagata acaggaatga acttaatttg catgtggtta
      661 cagccctgtt ttgggagaac tctaaaccct tagatcaaca actgcctcaa tttttaaaga
      721 ttcgtcaaca acaacaaacc aatggcaatg taaaagcaac gtctataaaa atttggcaag
      781 atggtgttat tgagacccaa actgcagcac tgttggagcc ttattctgat cgcagtgatg
      841 gttatagggg tgatttacag aacactccag ataatctcaa tacggcagtt gttgcattgg
      901 atgcagccgg ttttcagatc cactttcatg ccattgggga cagggcaatt cgagtatcat
      961 tggacgcgct tgaggaggca atcaatagca atggtactcg cgacagtcgt catcatttat
     1021 cacatattga attatttaac ccagctgaca taccccgttt tgcagaaatg aatgtgatag
     1081 caaattttca acccctgtgg gctatccaag atgaatatat tactgatctc acttggcctc
     1141 gtttaggaga agagcgaagt aaatggctat acccgattgg tagtatgcaa agaacaggtg
     1201 ctaaggttgg gtttggaagt gattggtatg ttacttcagt caatccactt gatggtattg
     1261 aaactgcagt tactcgctta gatcccaacg gattgactga tgtcccattg ggtcagaatg
     1321 aagaaattag tcttgcacaa gccattgaga actacactct gaatagtgct tatgttaatt
     1381 ttctggatca tagagtgggc tcagttgagg tgggtaaaca agcggaccta gtgattttgg
     1441 atcgaaattt gtttgctatc cccacatccg aaatcaatga ggcaaaagtt attgcaacgc
     1501 tgtttgaagg tcgtttggtt tatgggacat taaagataca atagagatct tcatgccata
     1561 aagcctatgc actaccagtc gatgtacgct tgaaccaata ataaccctga gctattgaaa
     1621 cgccaacgct cacgttctgt ccatcgattt cttcgatcgc aaaaaaccgt tcttttaact
     1681 ctggtgcaaa aaacttctga atatcttctc gggaaacccg tgtcggacca attatagatt
     1741 gaggttcatc gggtgtgcgt tgtagccagt gcaggataag gaactcgccg tcatcagcaa
     1801 gagcctgcat aacgcgttct cggtagagtg gatagcggtc aggattcatg ttatgcaata
     1861 gcccgtggtc aagtaccagg tcaaacttat cggtcggctg ccattcggtg acgtcagctt
     1921 caatggttgt cagtttcaat ccagctgccg tagcacgtaa gcaggtcatc tctagtgccg
     1981 cgggcatgaa atcgaggctg gttacatccc agttttcacc ggcgaggtat agggaatcgg
     2041 tgccggcgcc gcaaccgatg tccagtgctt tgccgggttt accacgctgt ccgacgatat
     2101 cgcgaaggaa cagcgtcggt tcatcatgtg cccatggtaa cttgctgcga tcagagccag
     2161 cccaatcata gatcttatag aagttatctt tgtcgtgtac tttttctatg gttccatctc
     2221 tcctattctt aggattatag attataagaa cttcacaaaa ctctcaaaca tctcaaaagc
     2281 agttgtctta aaccatccac ctagtcaaac aagtaattat ttattatgat atgagctctg
     2341 cttaatttta ttacataacg ttaaaaaaga gagcaaaaat tacgaaagat caatgattat
     2401 ggaggactgg catggcagat gaatcaacac aagacaatta tgcaggagtt tgggataccg
     2461 gacttggatt tggtaaaaag tcagcactta ttg
//
LOCUS       DASCTH010000161         4271 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000235261, whole genome shotgun sequence.
ACCESSION   DASCTH010000161 DASCTH010000000
VERSION     DASCTH010000161.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000235261"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="QGH35_03440"
     CDS             complement(<1..268)
                     /locus_tag="QGH35_03440"
                     /inference="COORDINATES: protein motif:HMM:NF039476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HJL81621.1"
                     /translation="MKKRLIIWEVGSFFWVILAGSALHFSFELSEFWTPLALFSAVNE
                     STWEHLKMYFWPGLIYALIQYTYTRDIANNYWLAKLISLIATPLG"
     gene            complement(288..881)
                     /locus_tag="QGH35_03445"
     CDS             complement(288..881)
                     /locus_tag="QGH35_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HJL81622.1"
                     /translation="MDHRNTKLSLLLLLSFPMILNAHEANSLPYGPFLSGITHPVLGF
                     DHFLAMVSVGMVSAQIGGAAIWSVPLTFVSVMFLGGLMGISLGGMSGYEIGIALSVLF
                     LGAALAADKEITRLFAMIAVAIFAVFHGYAHGQEIPVIAEPKPYIAGFMAGTIMLHIT
                     GLVLADISTHYRKGKVILRCTGFLIALVGVYFFTISV"
     gene            complement(895..1878)
                     /locus_tag="QGH35_03450"
     CDS             complement(895..1878)
                     /locus_tag="QGH35_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJL81623.1"
                     /translation="MIDHTNLKVSPDLSLYKSLEGYEKIMSWYERLKNRIQVDHDSLF
                     INTRFGTTHVIAAGDKEAKPVLLIPGIAGCAPLWRHQINDLSSHYRIFALDVVGQPGK
                     SDPNPLSIFNDDYTHWLEDVIKGLGIEKPHCIGVSTGGTTAMDMAIVKSELIDKVVMC
                     GPTGLARARLPFHIWFSRAMAKKKDADVLEDEITASSFSKPRSGKTFGSFDRQLARGM
                     ALCTRFYRVDKSLGIYSERTSRINFIKGLKVIGKFFFSVEKKRLKKFRNDGLLILGEF
                     EVLYDPKKISKKIKKLIPNLKIQIINDAGHAAVYDQPNEVNSLVISFLSNN"
     gene            1996..3513
                     /locus_tag="QGH35_03455"
     CDS             1996..3513
                     /locus_tag="QGH35_03455"
                     /inference="COORDINATES: protein motif:HMM:NF018012.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HJL81624.1"
                     /translation="MMQRERRKKSKGNLVNQLPWQEVMTEFSALEIISEDEIESIHHA
                     SLDVLERVGMKILHQDARKIFKDAGLDVDESSQMVHFDRNLVLSNISKAPKEFTLRAR
                     NPKRDLKIGGKRIVFSAIGGPAFCSDLDRGRRRGTFEDLCNYMKIIQSLNIIHQEGGM
                     AFETIELPSSTRHLDFYLAQFQLLDKNCQSSSMGKVRTKDCIDMHRIAFDCTREELLE
                     NPSVMGIINTNSPMQLDIPMSEATIELARAGQAVCVTPFTLSGAMAPIKQAGTLVQQN
                     AEILAVVTLTQLVRSSAPIIYGSFSTNVDMRTGSPAFGTPEYIRSTIAGGQLARFYGF
                     PYRSSNTNASNSVDAQAIYESGMSLWATIMSHANLVNHAAGWLEGGLTASFEKLIIDA
                     EMLQTISESLKPIEVNSYNLGVETIESVGPGGHFFDTDQTMKHYSTEFYTPMISDWEN
                     FENWEKNGSITTLERANKTWKNLLKHYEQPTLDPSVNEALIAFVAKRKEEINNSR"
     gene            complement(3521..3607)
                     /locus_tag="QGH35_03460"
     tRNA            complement(3521..3607)
                     /locus_tag="QGH35_03460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3571..3573),aa:Leu,seq:taa)
     gene            complement(3620..3690)
                     /locus_tag="QGH35_03465"
     tRNA            complement(3620..3690)
                     /locus_tag="QGH35_03465"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3656..3658),aa:Cys,seq:gca)
     gene            3836..>4271
                     /locus_tag="QGH35_03470"
     CDS             3836..>4271
                     /locus_tag="QGH35_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HJL81625.1"
                     /translation="MKKLTLLLYIVIFCTGTTNMVNAMNPFFTDYTTPYQIPPFEEIK
                     MEHYQPAFEQGMKEHIDEINAIADNKAAPTFENTIVAMERTGKTLDKVADVFFNLLGS
                     NTNDEMDSLAQLISPQLSAHSDKILLNKKLFQRIKSIYEERHS"
BASE COUNT         1409 a          868 c          778 g         1216 t
ORIGIN      
        1 ttccaagtgg tgttgctatc agtgaaatta attttgctag ccagtaatta ttagcaatgt
       61 ctctcgtata agtatactgg atgagtgcat agattaatcc aggccaaaaa tacattttta
      121 gatgctccca tgtactttcg tttactgctg aaaaaagggc aagaggtgtc caaaattcac
      181 tgagttcaaa agaaaaatgt agagctgagc ctgctaatat aacccaaaaa aaactgccta
      241 cttcccagat aatcaatctt tttttcataa ttcaaccctc ctcactgtca aacagaaatt
      301 gtaaaaaaat aaaccccaac aagtgcaatc aaaaaacctg tacaccttaa aataactttc
      361 ccttttctat aatgtgttga aatgtctgct agtactaagc ctgtaatatg taacattatg
      421 gtccctgcca taaaaccagc aatatagggt ttgggttctg caatcaccgg tatttcttgt
      481 ccgtgagcat aaccatgaaa tacagcaaaa atagctacag caatcatggc aaataagcgg
      541 gtgatctctt tgtcggctgc taatgctgcc ccgagaaata aaacagataa agcaatacct
      601 atctcataac cgctcattcc ccctaaacta atacccatga gcccacctaa gaacatgacc
      661 gacacaaatg ttaaggggac agaccaaata gcagccccac ctatttgggc actcaccata
      721 cctacactaa ccatagctaa aaaatggtca aaaccgagca ccggatgagt aatgccacta
      781 agaaaaggcc cataaggtaa actgttagcc tcgtgagcat ttaggatcat aggaaaagat
      841 aaaagtaata ataaactaag tttagtattc cgatgatcca taaagtgtta ggtattaatt
      901 attagataaa aaggagataa ccagactgtt tacttcattc ggttggtcat aaacagccgc
      961 atgaccagca tcattaatga tttgaatctt taaattaggt attaattttt ttatcttttt
     1021 tgaaattttt ttaggatcat ataaaacctc aaattcacca agtatcaaaa gaccgtcgtt
     1081 cctaaacttc tttaaacgtt ttttctcaac agaaaagaag aatttaccga ttacttttaa
     1141 gccttttata aaattaattc ttgaggttct ctcgctataa atccccaacg atttatcaac
     1201 ccgataaaac cgagtgcaca aagccatacc tcttgccaac tgcctatcaa atgaaccaaa
     1261 tgtttttcct gatcttggct tggaaaatga agacgctgtg atttcatctt cgagaacgtc
     1321 tgcatctttt ttcttagcca tcgctcttga gaaccatata tgaaatggca atctagctct
     1381 ggcaagtcca gtgggtccac acatcaccac tttgtctatt aattctgact tgacaatagc
     1441 catatccatt gcagtcgtcc ccccagtgct gactccaata cagtgaggtt tttctatacc
     1501 taaacctttg attacatctt ccaaccaatg ggtataatca tcattgaaaa tggagagtgg
     1561 atttggatca ctttttcctg gttgccctac tacatcaagg gcgaagattc tataatggct
     1621 agataaatca ttgatttgat gacgccataa aggggcacaa cctgcaatcc ctggaatcaa
     1681 taatacgggt tttgcttcct tatcgcccgc agcgataaca tgcgttgttc caaaccgggt
     1741 attaataaat agagaatcat gatcaacctg aattctattt tttaatcgtt cataccaaga
     1801 cataattttt tcatatcctt caagtgactt gtagagactc aagtctggtg aaacttttaa
     1861 attagtgtgg tcgatcatct taaattagaa atttgttctt catttaattc ggtagagctg
     1921 atcatatgga tttataatta tttagctgta tcgagggcta taattgaaga gaagatttaa
     1981 aattcagaaa atctgatgat gcaaagagaa cgaaggaaga aatcaaaggg gaatttggtc
     2041 aatcaacttc cttggcagga ggtaatgact gagttttctg ctctggaaat tattagtgag
     2101 gatgagattg aatctattca tcatgcatcc ctcgatgttc ttgaaagagt tggcatgaaa
     2161 atcttacatc aagacgcaag aaaaatattt aaagatgcag gtttggatgt cgatgaatcc
     2221 tcacaaatgg tccattttga taggaatttg gtattaagta atatttcaaa ggcacctaaa
     2281 gaatttacct tacgagcaag aaatcccaaa cgagatttaa aaatcggggg aaagcgtatt
     2341 gttttcagtg ccattggtgg cccagctttt tgcagtgatc tcgatagagg aaggcggaga
     2401 ggcacttttg aggatctatg caactatatg aagatcattc aaagccttaa tattattcat
     2461 caagaaggcg gcatggcatt tgagactatt gaattacctt catcaacaag acaccttgat
     2521 ttttatttag cccaatttca attattggac aaaaactgtc aatcatcttc catggggaaa
     2581 gttagaacta aggattgcat tgatatgcac cgcattgcct ttgattgcac tagagaggaa
     2641 ctgctagaaa atccatctgt tatgggaatt attaatacaa attctccgat gcaactggat
     2701 atcccaatgt cagaagcaac cattgagctt gcaagagcag gacaagcggt ttgtgtaacc
     2761 ccatttactc tatcaggggc tatggcacca attaagcaag ctggaacact ggtgcagcaa
     2821 aatgcagaga ttttggcggt ggtaacctta actcagttag ttagatcaag tgctcccatt
     2881 atttacggtt cattttctac caatgtcgac atgagaacag gatcccctgc ctttggtacg
     2941 cccgaataca ttagatccac gattgctggt ggacaacttg caagatttta tggttttcca
     3001 taccgaagta gcaacaccaa tgcttccaat tctgttgatg cacaagctat ttatgaaagt
     3061 ggaatgtcat tgtgggcaac catcatgagc catgcaaatt tagtgaatca cgccgcaggg
     3121 tggcttgaag ggggtctaac agcttctttt gaaaaattga tcatcgatgc agaaatgctg
     3181 cagacaattt ctgagtcttt gaaacccatt gaggtaaact cttacaatct aggggtggag
     3241 acgattgaaa gtgtcggtcc tggtggacat ttctttgaca ccgatcaaac catgaaacac
     3301 tactcaaccg aattttacac tccgatgatt tctgactggg aaaatttcga aaattgggaa
     3361 aaaaatggat ccatcaccac acttgaacga gccaataaga catggaaaaa tttgttgaag
     3421 cactacgaac agccaaccct agatccatct gtcaacgaag ctttaatagc ctttgtggca
     3481 aaaagaaaag aggaaatcaa taattcgaga taattgaatt tggtgcccag ggccggactc
     3541 gaaccggcac gtcctttcgg acaacggatt ttaagtccgt agcgtctacc aattccgcca
     3601 cccgggccca aaattcatga ggcaaggatc ggaatcgaac cgacgtccac ggctttgcag
     3661 gccgctgcat aaccactctg ccaccttgcc ataaaaaaac agttaaatta taccttttca
     3721 atagtatttt cgcttgcaat ctacagcatc ctaatgaaat aagacatcat ttttatagac
     3781 atctacttta aatatttcta gagtaatggg tcctaatcat taattcagat agaaaatgaa
     3841 aaaattaaca ctgctcttgt atatagttat tttttgcacc ggcactacaa acatggtcaa
     3901 tgctatgaat cctttcttta cagactatac aactccctac cagatccctc cttttgaaga
     3961 aataaagatg gaacattatc aaccagcatt cgaacaaggc atgaaagaac acatcgatga
     4021 aattaatgca attgctgata ataaagctgc acctacattt gaaaacacca tcgttgccat
     4081 ggaaagaact ggtaaaaccc tagataaggt tgcagatgta tttttcaacc tacttggatc
     4141 aaatacaaat gatgaaatgg atagtctcgc tcaactcatc agccctcaac tctccgccca
     4201 cagcgataaa atattactga acaagaaact ctttcaaaga ataaaaagca tttatgaaga
     4261 aagacacagt t
//
LOCUS       DASCTH010000162         1934 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000235955, whole genome shotgun sequence.
ACCESSION   DASCTH010000162 DASCTH010000000
VERSION     DASCTH010000162.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000235955"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="QGH35_03475"
     CDS             <1..378
                     /locus_tag="QGH35_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012488.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJL81626.1"
                     /translation="GSIGLGSAIDYLESIPFDLCQQHEMSLHNYALEKLKSVDGLQLI
                     GESSEKSPIISFIIENIHPHDIGTILNQKGVAVRTGHHCTMPLMDYFSIAGTIRASFS
                     FYNTIQEIDQMIDALDTACKMLA"
     gene            379..843
                     /locus_tag="QGH35_03480"
     CDS             379..843
                     /locus_tag="QGH35_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013738.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HJL81627.1"
                     /translation="MNQELSTLYQTTILDHGHHPRNFGKPKKYDQKLEGFNHLCGDKI
                     MIYCNYNRHENLNLSFESVSCVICTASASLMTAELENASRQQAIALIDQILNGLSNTS
                     LCPKGLSDQLLGLSGVVKFPSRIQCATLPWETTRKIISESGSFNEGENDARP"
     gene            830..1378
                     /gene="sufT"
                     /locus_tag="QGH35_03485"
     CDS             830..1378
                     /gene="sufT"
                     /locus_tag="QGH35_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HJL81628.1"
                     /translation="MQDRDEVIILSRDCETISVPYGEKNTLKKGTEVQIMQAMGGSYT
                     VYTSEGMFRINGYNADAIGKETEPPPFVPKNISDEEYESKIWEQMKTVYDPEIPINVV
                     DLGLIYACEIKKNDSNEYSVTIEMTLTAPGCGMADVLVEDIKERVEMLPRIAKVEVAL
                     VLDPPWSMEMMSETARLEAGLI"
     gene            complement(1381..>1934)
                     /locus_tag="QGH35_03490"
     CDS             complement(1381..>1934)
                     /locus_tag="QGH35_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HJL81629.1"
                     /translation="PTFERTFDQIGLHEDGYSTVDLTTSVMQTLSDREKEIIQMAVDN
                     IYLKFISKVGENRSMTNEEVDAIARGRVWTGEKALEIGLIDELGDIEDAIAAAGNLAK
                     LDEDDYGVKLIEREMDFGFNFGFSFDNDRMTKVISFLDFLGFSTASDTLPPIIKTFEQ
                     QSNLLNDFNDPRGIYYYCFCSVE"
BASE COUNT          644 a          386 c          365 g          539 t
ORIGIN      
        1 ggctccattg gactgggcag tgccattgac tatttggaat cgattccctt tgatttatgt
       61 caacaacatg aaatgagctt gcataattat gctctggaaa aactaaaatc ggtcgatggg
      121 cttcagttga taggggaaag cagtgaaaaa tcaccgatta tttccttcat tattgaaaac
      181 atccatccac acgatattgg aacaatatta aatcaaaaag gcgttgctgt tagaacagga
      241 catcactgta ccatgccgtt aatggattat ttttcaatcg ccggaacaat cagagcttcg
      301 tttagttttt ataataccat ccaggaaatc gaccaaatga ttgatgcctt ggatactgct
      361 tgtaagatgc ttgcttaaat gaatcaagaa ttatcaacat tgtaccaaac aaccatactg
      421 gatcacggcc accatcctag aaactttgga aaaccaaaaa aatatgatca aaaactggag
      481 ggttttaatc acctttgtgg tgataagatc atgatttatt gcaattataa tcgacatgaa
      541 aacctaaact tatcatttga aagtgtgagc tgtgtaatct gtactgcctc tgcttctctg
      601 atgacagcag aactagaaaa tgcttcaaga caacaagcaa ttgcattgat cgatcagata
      661 ttaaacggac tcagtaatac atctttatgt ccaaaaggac tgtcagatca actcttgggt
      721 ctcagtggtg ttgtaaaatt tccatccaga atccaatgcg ccaccctacc ttgggaaacg
      781 acccgaaaaa taatatcaga atctggatca tttaatgaag gagagaacga tgcaagaccg
      841 tgacgaggtc attattcttt caagggattg tgaaacaatc tccgttcctt atggcgaaaa
      901 aaatactttg aagaaaggaa cagaagttca aattatgcaa gcaatgggag gttcgtacac
      961 ggtttatacc agcgaaggca tgttcaggat aaatggctat aatgcagatg cgatcggtaa
     1021 agaaaccgaa ccaccacctt ttgtaccaaa aaatatatca gatgaagaat acgaatcaaa
     1081 aatctgggaa caaatgaaaa ccgtttatga ccctgaaata ccaatcaatg tcgttgatct
     1141 tggcttgatc tatgcctgcg aaattaagaa gaatgattct aatgaatact ccgttaccat
     1201 tgaaatgact cttactgccc caggttgtgg catggcagat gtactggttg aggacatcaa
     1261 agaaagagtc gaaatgctac ctagaatcgc taaagtggaa gtagcattgg tcctagaccc
     1321 gccctggagt atggagatga tgtcagagac tgccagatta gaggcgggtt taatctagtt
     1381 ttattctact gaacaaaaac agtaataata tatacctcta ggatcgttga aatcattcag
     1441 taggtttgat tgctgctcaa aggttttaat aataggaggt aatgtgtcag aagcagtgga
     1501 gaatccaaga aaatccaaaa aagatatcac cttggtcatc cgatcgttat caaaagaaaa
     1561 accaaaatta aatccaaaat ccatttccct ttcaatcaac ttgactccat aatcatcttc
     1621 atctagttta gccagattcc ctgctgcagc gattgcatcc tcaatatcac ccaattcatc
     1681 aatgagtccg atctctaaag ccttctcgcc cgtccatact ctacccctag caatagcatc
     1741 aacttcctca ttagtcatgg atctgttctc acccacctta gagatgaatt taaggtaaat
     1801 attatcaacc gccatttgga ttatttcttt ttccctatct gataaagttt gcattacaga
     1861 ggtagtcaga tcgactgttg aataaccgtc ctcatgaagc ccaatttgat caaaagttct
     1921 ctcgaaggta ggta
//
LOCUS       DASCTH010000163         2067 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000242235, whole genome shotgun sequence.
ACCESSION   DASCTH010000163 DASCTH010000000
VERSION     DASCTH010000163.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000242235"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="QGH35_03495"
     CDS             <1..543
                     /locus_tag="QGH35_03495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJL81630.1"
                     /translation="SYSKTLARINLQEDSKVLDLGCGTGELLKILEDLFPSSELTGID
                     LTEEMLAIAKQKLSNKVKLFLGSATSLPFDSKSFDWVIMSNMIGHLSDKKAVLKEAHR
                     VLKNLGKIVITDWTRDFQTMNLVHFYTKLINYSQYRSLRSAELESLLDDCDFDHLHTE
                     SYKINWFWGLYTIQAEKKKV"
     gene            complement(579..809)
                     /locus_tag="QGH35_03500"
     CDS             complement(579..809)
                     /locus_tag="QGH35_03500"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HJL81631.1"
                     /translation="MSKGKDQKKSIEKSLAELEKLVEELENGDLELDQALKKFEQGVK
                     LSRECKKTLEETEMKIKILMDNALKATDEELN"
     gene            972..1481
                     /locus_tag="QGH35_03505"
     CDS             972..1481
                     /locus_tag="QGH35_03505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJL81632.1"
                     /translation="MHGVGEKFPEFSMTAVDKNNEMIQVTSGDLNGWSVIYFYPKDFT
                     FICPTEIAEMDRLTDEGVQVIGISGDNEFCKQNWKQTNDLIQDVRHPLAADCGLKLST
                     ELGVVDTAEGVCLRATFILNSDGVIQHVTVNALDTGRNADETIRTLKALQAGGLTGCS
                     WNPGDEFVA"
     gene            1559..>2067
                     /locus_tag="QGH35_03510"
     CDS             1559..>2067
                     /locus_tag="QGH35_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJL81633.1"
                     /translation="MTKEYNASDIEVLSGLEPVQKRPGMYTDTDCPNHLAYEVIDNSV
                     DEAIAGHCKKIEVILHNDGSLQVKDDGRGMPVDDHPEEKVPAVEVILTRLHAGAKFSN
                     KNYRFSGGLHGVGVSVVNALSKNLEAWIKRNGHEYNLSFKNGERISELEVVGNVGKSN
                     TGTTIRFWP"
BASE COUNT          657 a          329 c          433 g          648 t
ORIGIN      
        1 tcgtattcaa agactcttgc tagaattaac ctacaagaag attcaaaggt tcttgatcta
       61 ggctgcggga caggagaatt gctcaaaatt cttgaggatt tgtttccatc atctgagctc
      121 accggaattg atttgactga agaaatgctg gcgattgcaa aacaaaaact ttccaataaa
      181 gttaagcttt ttttgggcag tgcaacaagc cttccgtttg attctaaatc atttgattgg
      241 gtcattatgt ctaatatgat tggtcatctt tcagacaaga aggcagtgct aaaggaagct
      301 catagagtac taaaaaacct agggaaaatt gtgattactg attggacacg tgattttcaa
      361 acaatgaatt tagttcattt ctatacaaaa ttgattaatt attcacaata tagaagttta
      421 agatcagcag aattggagag tcttttggat gactgtgatt ttgatcatct gcacacagag
      481 agctataaga taaattggtt ttgggggctc tatactattc aggcagaaaa aaagaaagtt
      541 taaccaatga aaaacagttc aagaggtgtt tgtagcttct agtttaactc ttcgtctgtt
      601 gctttcaggg cattgtccat aaggattttt attttcattt ctgtttcttc caaagttttt
      661 ttgcattctc tggataactt aaccccctgc tcaaattttt ttaaagcttg atctaattca
      721 aggtctccat tttcaagctc ctcgaccagc ttctcaagtt cagccaagct tttttcaatc
      781 gatttttttt gatctttccc tttactcatt aactctaccc aattgatttg atgatagtag
      841 agtattagat ttgcacagac tttaagtatc gtcaatcaat taaagaagtt gagaaagagt
      901 attgctgcgg taagatatct gagttagaat tagaatgatt ctaatcaata aaatttttgg
      961 ggagttaaat tatgcatggt gttggtgaaa aatttccaga attctctatg acagcggtag
     1021 ataagaataa tgaaatgata caggttacat ctggtgatct gaacggatgg tcagtgattt
     1081 atttttatcc aaaggatttt acattcattt gtccaactga aatagctgag atggatagac
     1141 ttactgatga gggtgttcag gttattggta ttagtggtga taatgagttt tgtaaacaaa
     1201 actggaaaca aacaaacgat cttattcaag atgtcaggca tccactagca gcagactgcg
     1261 gtcttaagtt atcaactgaa cttggagtgg tcgatactgc tgaaggtgtt tgtcttagag
     1321 cgactttcat cttaaacagt gatggtgtga ttcaacatgt aacagtcaat gcattagata
     1381 ctggcagaaa tgctgatgaa accattagaa cactaaaagc tcttcaggca ggtggtctta
     1441 ctggttgttc atggaatccc ggcgacgaat ttgttgcata aagcataaga caatttaaaa
     1501 aaaactcttt ttaaaatgat cttgattgat tcttgtactt cgagaataat tgaggaccat
     1561 gacaaaagaa tataacgctt cagatattga agtattaagt ggactcgagc cggttcaaaa
     1621 aaggcctggg atgtacaccg atacagattg tcctaatcac cttgcctatg aagtgatcga
     1681 caacagtgtg gatgaggcaa tagctgggca ttgtaaaaaa attgaagtaa ttttacataa
     1741 cgatggttcc ttgcaagtaa aggatgacgg gagagggatg cctgttgatg atcaccctga
     1801 agaaaaagtt cctgccgttg aagtgatttt gactcgattg catgcaggtg caaaattttc
     1861 taataaaaac tatcgattct caggcggact ccatggggtg ggtgtatccg tagtcaacgc
     1921 actttcaaaa aatcttgaag cctggattaa gcgaaacgga catgaatata acctcagttt
     1981 taaaaatggc gagagaatat ctgaactgga agtagttggg aatgttggta aatcgaatac
     2041 gggaacaacc ataagatttt ggcccaa
//
LOCUS       DASCTH010000164         4043 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000243246, whole genome shotgun sequence.
ACCESSION   DASCTH010000164 DASCTH010000000
VERSION     DASCTH010000164.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000243246"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="QGH35_03515"
     CDS             complement(<1..218)
                     /locus_tag="QGH35_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81634.1"
                     /translation="MKAFFEKTINEKIRLVIALLFLLPPFGLTSAHDDPSSISKSSDD
                     EIVLRRTTLIVRDVEHSLNLYRDSIGMEV"
     gene            complement(251..1702)
                     /locus_tag="QGH35_03520"
     CDS             complement(251..1702)
                     /locus_tag="QGH35_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008490916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HJL81635.1"
                     /translation="MATIDETINEAFKPLASAFNDLVFYSVPIGKSQLPLIVVWLIAG
                     ALYFTFYLRLINVRGFAHAVRIVLGKEDKADKAPGEVSHFRALTTAVSGTVGVGNITH
                     VAVAISIGGPGAAFWLIVAGFLGMASKFVECTLGVKYRQVNADGSVSGGPMYYMDKGL
                     AEQNLPRLGKVMAWYYAICIVVGCLGVGCMFQANQAYVQFVNVTGGAEGFFASRGWLF
                     GSILALLTGLVIIGGIKSIARVTSKLVPFMALLYVGSALVVIGANFSAIPSAVSSIIT
                     NAFSPEGVTGGMIAVLILGFRRAAFSNEAGIGSSAIAHAAVKTDEPLTQGFVAMLEPF
                     IDTVVICTITALVIILTVYDPAMLNEGTIRGVELTSSAFASVIPWFPSVLAVVVMLFA
                     YSTMISWSYYGLEGFVYMFGSKRRTKRIFNTVFCLFVVLGCTVQLDAVLDFSDAMIFA
                     MALANMLALYLLGPNVKRELDAYWARLKTRSSS"
     gene            complement(1727..3430)
                     /locus_tag="QGH35_03525"
     CDS             complement(1727..3430)
                     /locus_tag="QGH35_03525"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81636.1"
                     /translation="MKSVGSVIFIVSLVVLIFSWVAWDDQSADIIFIGDHIITVDSTA
                     VNVTAVAVAGQNIVATGSAEEVLKLKKSSTRIVELGNNALVPGFIDAHGHMTGVAKLT
                     EIIDLASPPVGRVENIDDIVALIESKIDTQKLVPGTWVLGFGYDDSLLEEKRHPNRDD
                     LDRVSSDHPVLLTHVSGHLATVNSAALRQQKIDQNTENPPGGVIRRRPGSREPNGVLE
                     ETAMGLFSRNLMAPMKDDKFEHLVRQAVQRYTSYGITTIQDGGTNMADIERLRAAANR
                     KPYAADVVVFPWSNSFDDNQLAAIEAEPSYTNGLRLGGIKFGLDGSPQGRTAFLSQPY
                     NEGPPGAAPDYRAYPTYPAEKLNPKIARLLERGTPTLVHANGDAAIDMLLDGVTAAFD
                     NKELSDHRTVIIHAQLMRKDQLERTKKLGLVPSYYSAHPFFWGDWHRRSFGEERAAFI
                     SPAAETARMQIPFTIHNDAPIVPPDMMRLMWVAVNRKTRSDFVLGPDQRLTPMQALHA
                     ITLGAAYQYFEENKKGSITPGKQADLVILERNPLLANPDTLKDISIIETFARGQSVFK
                     K"
     gene            complement(3427..>4043)
                     /locus_tag="QGH35_03530"
     CDS             complement(3427..>4043)
                     /locus_tag="QGH35_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HJL81637.1"
                     /translation="HSETLMQEALRRNQGIRLRHRPSHAFYMTVPGHEQGICITDAVI
                     NVLPRVKQKIEIARNVIELMHALGIPRPRVALVSGAEEPIPEMPSSIEAVEVIKEVEK
                     GALRGADVAGPMALDVAISEQAATIKGVNNAVAGKADVLLVPNLEAGNILFKQMVYSM
                     SATAAGLVLGMKVPIMLTSRADPPEARLASAALASIYAAHRRTK"
BASE COUNT         1153 a         1027 c          884 g          979 t
ORIGIN      
        1 acttccatgc ctatggagtc ccggtacagg tttaatgaat gctcaacgtc tctcacaatt
       61 aaagtggttc tccttagaac aatttcatcg tccgaagatt tgctgattga agatgggtcg
      121 tcatgagcgg aggtaaggcc gaaaggagga agaagaaaca acaacgcaat aactaacctt
      181 atcttttcat taatggtttt ttcaaaaaaa gctttcattt ggcccctttg ttttaatctt
      241 actctaccag tcaactagat gatcttgttt ttaacctggc ccaataagcg tccagttccc
      301 gcttcacgtt tggccctagc aagtacagcg ccagcatatt ggcaagcgcc atcgcgaaga
      361 tcatagcatc tgaaaaatct agaacggcat caagctgcac agtacaaccc aacaccacaa
      421 acaaacaaaa aacggtgttg aaaattcttt ttgttcggcg cttggaccca aacatataaa
      481 caaagccctc caacccataa taagaccaag agatcatggt tgaatacgcg aacaacatta
      541 ccacaacagc aagcacgctt ggaaaccaag ggataacact ggcaaatgcg cttgatgtca
      601 gctcaacacc gcggatggtt ccttcgttca gcattgccgg gtcgtagacg gtgagaataa
      661 taacaagtgc ggtaattgtg caaatgacaa cagtgtcaat aaacggctcc agcatggcca
      721 caaacccctg tgtcaggggc tcgtcagttt taactgctgc atgggcgatg gccgaagacc
      781 caattcccgc ctcattggaa aaggccgcgc ggcgaaagcc taaaatgaga accgcgatca
      841 tgccgccggt gaccccttca ggactgaacg cgttagtgat gatcgacgaa acagcggagg
      901 gaatggcaga aaaattagcc ccaatcacca ccagcgcaga gccaacgtac aagagcgcca
      961 taaagggcac aagctttgag gtgaccctgg caatgctttt aattccgcca atgatgacta
     1021 gaccggtgag tagagccaag atcgaaccaa acaaccaccc acgactagcg aaaaacccct
     1081 cagcgccgcc cgtgacattg acaaattgca catacgcctg gttggcttgg aacatgcaac
     1141 ccaccccaag acagccaaca acaatacaaa tggcataata ccaagccatc accttaccca
     1201 aacgaggcaa gttctgctca gcaagacctt tgtccatata atacatgggc ccgcctgaga
     1261 cagacccgtc agcattgacc tgacggtatt taactcccaa agtgcactca acaaacttag
     1321 acgccatgcc taaaaagccg gcaacaatga gccaaaaagc agcccccggg ccaccaatag
     1381 atatagcaac agcaacatgg gtgatgttgc caacacccac agtgccagat acagcagttg
     1441 ttagcgccct gaagtgtgaa acttcgcccg gtgctttgtc tgctttgtct tcttttccga
     1501 gaacaatccg aaccgcgtgt gcaaatcccc ggacattaat gagccgtaaa taaaacgtaa
     1561 aatacaaagc acctgcgatc agccaaacca caatcaaagg gagttgcgat ttcccaattg
     1621 gaaccgaata aaaaactaaa tcattaaacg ccgacgctaa tggtttaaaa gcttcgttaa
     1681 ttgtttcatc aatggttgcc ataaaactaa cctcaaaaat tcattgttat tttttaaata
     1741 ccgactgtcc ccgagcaaag gtttctataa ttgaaatgtc tttcagcgta tcggggtttg
     1801 ccaacagcgg attacgctct aggatcacta gatcagcttg tttgccaggg gtaattgatc
     1861 cttttttatt ttcttcaaag tattgatagg cagcacccag cgtaattgca tgcaaggctt
     1921 gcattggtgt caagcgttgg tcgggcccta aaacaaagtc actgcgcgtt tttctgttaa
     1981 ctgcaaccca cattaaacgc atcatatccg ggggaacaat cggcgcatca ttgtgaatgg
     2041 tgaacggtat ttgcatgcgc gccgtttctg ctgctgggct aatgaacgcc gcccgctctt
     2101 caccaaagct tcggcgatgc caatcgcccc agaagaaggg gtgtgctgaa tagtaagaag
     2161 ggaccaggcc tagctttttg gttctttcaa gctggtcttt gcgcatgagc tgtgcgtgaa
     2221 taattacggt acggtggtct gacagttctt tattatcaaa ggccgctgtg acaccatcga
     2281 gcaacatatc aatggcggca tcaccatttg catgcactag cgtaggagtg ccccgttcaa
     2341 gtaatcgagc aattttgggg ttgagttttt ctgctggata agtcggataa gctcgataat
     2401 cgggagccgc ccccggtggg ccctcattgt aaggctgaga aagaaaagcc gtgcggcctt
     2461 gtggagatcc gtccagccca aactttatac cccccagtct taagccattg gtgtaggagg
     2521 gctcggcctc aatggccgct aactgattat catcgaatga gtttgaccag ggaaacacca
     2581 ccacatcagc agcatagggt tttctgtttg cagccgcacg cagccgctca atatcggcca
     2641 tattggtgcc gccgtcttgt atcgtggtga tgccatagct tgtgtagcgt tgtactgcct
     2701 gtctgactaa atgctcaaac ttgtcatctt tcataggagc cattagattt cttgaaaaca
     2761 aacccattgc tgtttcttcc agcaccccgt tgggttctct cgaaccaggc cgccgacgta
     2821 ttactcctcc gggagggttt tctgtgtttt gatctatttt ttgttgccta agagctgctg
     2881 aatttacagt ggctaaatgt ccagaaacat gggttaacag cactgggtga tcagacgaaa
     2941 ctcgatcaag atcatcacga tttgggtgtc gtttttcttc aagcagcgag tcatcgtaac
     3001 caaacccgag cacccaagtc ccaggcacga gtttttgtgt gtcaatttta gactcaatta
     3061 aagcaacgat atcatcaata ttctcgacac gaccaactgg tggcgaggca aggtcaatta
     3121 tttcggttaa cttagcaaca ccagtcatat gaccatgggc atcaataaac ccaggaacca
     3181 aggcattgtt cccaagctca acaatccgcg tggagctctt ttttagcttt aaaacctcct
     3241 ctgctgagcc ggttgccaca atgttttggc cagccacagc cacagcggtc acgtttacag
     3301 ctgttgaatc taccgtaata atatgatccc cgataaaaat aatatctgcc gattggtcgt
     3361 cccaagcaac ccaagaaaaa attaaaacaa ccaagctcac aataaaaata acagagccca
     3421 cggacttcat tttgttctcc tgtgggcggc ataaatagag gccaacgctg cagaagcaag
     3481 cctcgcttca ggagggtcag cgcgtgaggt aagcattatg ggaaccttca taccaagcac
     3541 taaaccagca gcggtggcgc tcatagaata aaccatctgc ttaaataaaa tattcccagc
     3601 ctctaaattt ggcactagta agacatctgc tttgccagca actgcattgt tcacgccttt
     3661 tattgtcgcg gcctgttctg aaattgccac atccagagcc ataggcccag cgacatcagc
     3721 gccacgcaaa gcaccttttt caacttcttt gataacctca acagcctcta tgctggaagg
     3781 catttcaggt attggctctt cggctccaga aaccagagca actcgcggcc taggtattcc
     3841 aagtgcgtgc atgagctcta taacattacg tgcaatttca attttttgtt tcacccgagg
     3901 caaaacatta ataacagcat cagttataca aatgccctgc tcatgaccag gcacagtcat
     3961 ataaaaagcg tggctggggc gatgccttaa acgaattcct tgattacgac gtaaggcttc
     4021 ctgcattaaa gtctctgagt gaa
//
LOCUS       DASCTH010000165         1709 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000245772, whole genome shotgun sequence.
ACCESSION   DASCTH010000165 DASCTH010000000
VERSION     DASCTH010000165.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000245772"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="QGH35_03535"
     CDS             <1..539
                     /locus_tag="QGH35_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014615.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HJL81638.1"
                     /translation="LLSSTFDSHGKSMMGHEPKQLVELAERYSPEIIGYGANCGIGAS
                     ELIGSILCLAKSRNNQAMHLVAKANCGIPEYRDGEIIYNGTAEIMASYACYARKLGAT
                     IIGGCCGTKQEHIKAMADALEANDMDCPIELDEIIKSLGEMTKGNILMIEKYLNLGNS
                     SKVSPVNPRRKRRRRSKN"
     gene            complement(561..1676)
                     /gene="ald"
                     /locus_tag="QGH35_03540"
     CDS             complement(561..1676)
                     /gene="ald"
                     /locus_tag="QGH35_03540"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HJL81639.1"
                     /translation="MLIGVPKEIKSDEYRIGLTPESVAILTNGGHQVMVLANAGLGIG
                     KTDQDYLSAGAEIVATRDEIFERAEMIVKVKEPQPDECTLLRSGQILFTYLHLAPDPE
                     QTNALVESGVSAIAYESVMAEDHRYPLLTPMSAIAGRLAVQAGASCLEKAKGGSGILM
                     GGVANVDPAKVVILGGGVVGYNAIEIALGMQADVTVLDKSETRLDYLESIFGSNLEAV
                     LADTASNETFITTADLLIGAVLVPGASAPKIISRKVLKKMKPGSVFVDVAIDQGGCSE
                     TSKPTTHSNPTYIEEGILHYCVANMPGAVPLTSTYALNQATLPYVKDLANQGLVQALT
                     HNQGFCEGLSIHQGKVTIRAVADELGFDYTEPKKALI"
BASE COUNT          501 a          383 c          343 g          482 t
ORIGIN      
        1 ttcttttatc tagcactttt gatagccatg gaaaatcaat gatgggtcat gagccaaaac
       61 aacttgttga attagctgag cgatattcac cggagatcat tggatacggt gccaattgtg
      121 gcattggagc atcggagttg attgggtcaa tattatgtct tgcaaaatcc agaaacaacc
      181 aagccatgca ccttgttgca aaggcaaact gcggaatacc tgagtataga gacggtgaaa
      241 ttatctataa cggcaccgct gaaataatgg cctcctatgc ttgctatgcc agaaaattag
      301 gtgccacaat tatcggtggc tgctgtggaa caaaacaaga gcatattaag gcaatggctg
      361 atgccttgga agcaaatgat atggactgcc cgatagaatt ggatgaaata ataaagtctc
      421 taggtgagat gacaaaaggc aatatactta tgattgaaaa gtacttgaat ctgggaaatt
      481 catccaaagt cagtccagtc aaccccagaa gaaaaagaag gcgtcggtca aaaaactaaa
      541 cctcatccac tatcagccat tcagattaat gcctttttag gttctgtgta atcaaatcct
      601 aactcatcag ccactgccct gatggtaact tttccttggt ggatactcag accttcacag
      661 aatccttgat tgtgagtcaa agcttgcact aagccttggt tagctaaatc tttaacatag
      721 ggcaatgttg cctgattcag tgcataagtt gaagtcagag gcacagcacc aggcatgttc
      781 gctacacaat aatgtaagat cccttcttct atataggtgg gatttgaatg tgtggtcggt
      841 ttactggtct cagaacaacc tccttgatca attgcaacat cgacaaacac agatcctggt
      901 ttcatttttt tcaacacttt tctagatata attttgggag cggaagctcc tggtactaag
      961 acagcaccta ttaaaagatc ggcagtagtg ataaaagtct cattggatgc tgtatcggcg
     1021 agaactgctt ctagattgct tccaaaaata ctctctagat aatcaagtcg tgtctctgat
     1081 ttatccaaga ctgtgacatc ggcctgcata ccaagggcaa tttcaatggc attgtagcca
     1141 accacgccac ctccaaggat aaccactttt gcaggatcaa cattggcaac accccccatt
     1201 agaattccag acccaccttt ggctttctcc aaacagcttg ctcctgcttg tactgctaat
     1261 ctacctgcaa ttgcactcat gggtgttaat aaaggatacc tgtgatcctc tgccattaca
     1321 gattcatagg ctatggcact aacccctgat tccaccaaag cattggtttg ttcaggatct
     1381 ggtgctagat ggagataggt aaacaatatt tgacctgatc ttagcaaggt acactcgtcc
     1441 ggttgcggct cttttacttt tacaatcatc tcagcccgct caaaaatttc atctctggta
     1501 gcaactattt ctgctccagc tgatagataa tcttgatcag ttttgccaat gcctaaacct
     1561 gcgttggcca aaaccataac ttgatggcct ccattggtca ggattgcaac actttcagga
     1621 gtcaagccaa tccggtattc gtcggactta atttctttag ggacaccaat taacatatca
     1681 ccactcctat ttaactgtcg gcatcgata
//
LOCUS       DASCTH010000166         1676 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000245968, whole genome shotgun sequence.
ACCESSION   DASCTH010000166 DASCTH010000000
VERSION     DASCTH010000166.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000245968"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="QGH35_03545"
     CDS             complement(<1..1369)
                     /locus_tag="QGH35_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81640.1"
                     /translation="MNKTIFYSTAMAVLVLSCQYSYAASDLDLSINKHQQKFEDIALQ
                     IWEIAEMGYQEYRSSNLLKEALSEEGFRIQNNVANIPTAFIAEYGSGFPVIAILGEFD
                     ALPGISQSASPFKEKYKDNIAGHACGHHLFGAGSAWASVAIKEWLLKNKNSGTVRFYG
                     TPAEEGGSGKVYLAREGLFDDVDIVLHWHPGSQNHARPRTSNANKSAKFSFKGISAHA
                     AAAPDKGRSALDGVESMNMMVNLMREHIPQDSRIHYVITKGGLAPNVIPDEAEVYYYV
                     RHPKRKMVEELFKRVVKTAQGAALGTETTMSFEVMHGNYSLMPNDTLQEIMHEKLTSR
                     GGIRYSPKENEFANIIYQTLLNPKAKIGDQEDVLSYKPTHGYGSTDVGDVSWLVPTAG
                     ARISTWVPGTPAHSWQAVASGGTTIGLKGTKLAVQVLSETAKEIFLDPSVADKAKEEL
                     EKRVGK"
BASE COUNT          511 a          328 c          290 g          547 t
ORIGIN      
        1 ctttacccac tctcttttct aattcttctt ttgctttatc tgcaacagaa ggatcaagaa
       61 aaatttcttt agcagtttct gacaaaacct gcacagctag ttttgtgccc tttaggccta
      121 tagtggttcc cccagaagca acggcttgcc aagagtgagc aggagttccg ggaacccagg
      181 ttgatattcg tgctccagct gttggcacta accaactcac atctccaaca tcagtggatc
      241 catacccatg agttggttta taagataaaa catcttcctg atcaccaatc ttagcttttg
      301 gatttaacag tgtttggtag attatgttgg caaattcgtt ttctttaggt gaatatctta
      361 tgccacctct agaggttaat ttttcatgca tgatttcttg tagcgtatca tttggcatta
      421 aagaataatt cccatgcatt acttcaaaac tcatggttgt ttcagtgccc aaagcagctc
      481 cttgagctgt ttttacgacc cgtttaaaaa gttcttctac catttttctt ttgggatgcc
      541 ttacatagta ataaacttcg gcttcatctg gaatgacgtt aggagctaat ccacctttgg
      601 tgattacata atggattcta gagtcctgtg gaatgtgttc tctcataaga ttgaccatca
      661 tattcatgga ctcgactcca tctaaagctg atctcccttt gtctggggca gcggctgcat
      721 gagcagagat tcctttaaaa ctaaatttag ctgatttgtt agcattcgag gttctgggtc
      781 ttgcatgatt ctgacttcct gggtgccaat gaagcacaat gtcaacgtca tcaaatagcc
      841 cttctctagc caaataaact ttccctgagc cgccttcttc agcaggagtt ccataaaatc
      901 ttacagttcc agagttttta ttctttaata accactcttt aatagctaca gatgcccaag
      961 ctgagccagc accaaaaaga tgatgaccac aggcgtgccc tgcaatatta tctttatatt
     1021 tttctttaaa gggagaagcg ctttgagaaa tgccaggtag agcatcaaac tcacctaaaa
     1081 ttgcaataac tgggaatcct gatccatatt cagcaatgaa agcagttgga atattggcaa
     1141 cattattttg gatcctaaat ccttcttcag agagagcctc tttaagtaaa ttggaacttc
     1201 tatattcttg gtatcccatt tctgcaatct cccaaatttg taaagcaata tcctcaaatt
     1261 tttgttgatg tttattgatg cttagatcta ggtcagaagc ggcataagaa tattggcaag
     1321 ataaaacaag aactgccatt gcggttgagt agaaaatagt tttattcatt atatcctcgt
     1381 aaaaaaatat taagttatcc tactgatttt ttatcatata agaaacattt agagtttgta
     1441 gaaaaaaatg actcagtaaa ttttaatcac ttacctagtg gattcatagc atgagtcacg
     1501 gaaatgattt caattagttg tttctgagtt tctgaaaaaa caacgctctt gtaaggctac
     1561 acacagacca aaatagcaaa aaaatattaa atagaacaaa agactataca actataaata
     1621 tatttataat ataatgatat atatgggtat ttaaaatgac tcttataaaa atctga
//
LOCUS       DASCTH010000167         2242 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000251959, whole genome shotgun sequence.
ACCESSION   DASCTH010000167 DASCTH010000000
VERSION     DASCTH010000167.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000251959"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="QGH35_03550"
     CDS             complement(<1..873)
                     /locus_tag="QGH35_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81641.1"
                     /translation="MEDISKSKQNKALAFSTISFGVCFAVWTLFSIIGIQIKENLGLS
                     DSQFGLMLGTPILTGSLSRIVFGIWSEQYGGRIVYVGVMLTASLAAFLLSYSDTYLMT
                     LTAALGLGLAGGSFSVGISYVSKWYPKQKQGSALGIFGIGNMGAAVTTFLAPFVLVAF
                     GWQVVAQIWAGGLAIMAILFWLFTDNDPNLVKRKASGEKPKSFIEQLKPLKRIQVWRF
                     SLYYFFVFGAFVALALWLPRYLMGVYDLDIKTAGMLAAVFSIASVFRAYGGHLSDKYG
                     ARVIMYLTFGVAIVC"
     gene            complement(901..1629)
                     /gene="narI"
                     /locus_tag="QGH35_03555"
     CDS             complement(901..1629)
                     /gene="narI"
                     /locus_tag="QGH35_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HJL81642.1"
                     /translation="MITLDSVLFIAYPYVSIVVLLVGTIYRYTQEGYSISSLSAQFLE
                     NNVGFWGTTPFHIGIIMLAVGHLAIFLFPENVLLWNSDPTRLIVHEGLAFTFGAVVTV
                     AMTVLLIRRYTNPRLRAVTSPMDVVIGLIIYTQLVLGCWIALGYRWGSSWFAADLTPY
                     LRSIFTFDPEIAAISAMPWVIKLHVIGAFTIILLIPFSRLAHFLVAPLHYLWRPYQIV
                     IWYWDRKKIRDAGSAWQSNRPRNN"
     gene            complement(1626..>2242)
                     /locus_tag="QGH35_03560"
     CDS             complement(1626..>2242)
                     /locus_tag="QGH35_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81643.1"
                     /translation="EDAYPGAIFSLDQFIKLLPEHDLRMMQEIYTRSFDVQAITTLDI
                     GYVLFGDDYKRGQLLSHLNREHIQHHVDCGHELADHLPNVLKLIAVLEDGDLLIDMID
                     EILAPALHQMIAEFDDGRITQKHQAYEKHYKTLIESPSKLLATNTLYQYPLIALFEVL
                     KKDFPIAETFPETSDFLINITQENELEAGSKAFKKGTTNGELMQ"
BASE COUNT          698 a          483 c          460 g          601 t
ORIGIN      
        1 gcaaacaatt gctactccaa aggtcagata catgatcact ctggcaccgt atttatccga
       61 taagtgaccg ccataggcac ggaacacaga agcgatagag aataccgctg caagcattcc
      121 agctgttttg atatcaagat catagacacc catcaggtat cttggcaacc acagtgccag
      181 tgcaacaaaa gctccgaata caaagaaata atagagtgag aaacgccaaa cctggatccg
      241 tttcaaaggc ttcaattgtt cgataaagct ttttggtttt tcaccgcttg ccttgcgttt
      301 gactaaattt gggtcattgt ccgtaaataa ccagaataaa attgccatga tcgcaagacc
      361 accagcccaa atctgggcaa caacttgcca cccaaaagca accaagacaa aaggagcaag
      421 aaaagttgtt acagctgctc ccatgttccc aatcccaaaa atacctaggg cagacccttg
      481 tttttgttta ggataccatt ttgatacgta ggaaatccct accgaaaaag agccaccagc
      541 taatcctaaa ccaagggcag cagtcaatgt cattaaatag gtatcagaat aggacagtag
      601 aaatgcagct agtgaggctg ttagcatcac tccaacatag actatacgtc caccgtattg
      661 ttctgaccaa ataccgaaaa cgatacgact taaggagccc gtcagaattg gtgtgcccaa
      721 cattaaccca aactgactat cactaagtcc caaattttct tttatttgaa ttcctataat
      781 tgagaaaagt gtccaaactg cgaagcaaac cccaaatgat attgtgctga atgccagcgc
      841 cttgttttgt ttagacttgc ttatgtcttc catgatccta ctgcaagttt attatgaggt
      901 ttagttattg cgaggtctat ttgattgcca ggcgctacct gcatctctaa ttttctttcg
      961 gtcccaatac catatcacaa tttgatatgg tcgccataaa tagtgaagtg gtgcaactag
     1021 gaaatgagcc agtcttgaaa acgggataag caatataata gtaaacgcac caatgacgtg
     1081 cagcttgatt acccagggca tggcactgat ggctgcaatt tcaggatcga atgtgaatat
     1141 tgaccgtaaa tagggtgtca aatcagcggc aaaccaagaa gagccccaac ggtagcccaa
     1201 tgcgatccaa caacctagta cgagctgtgt atagataata agtccaataa cgacatccat
     1261 gggactggtc acggctctca atctagggtt tgtatagcga cgtattaaaa gcactgtcat
     1321 ggcaaccgtt actaccgccc cgaaagtgaa ggcaaggcct tcgtgtacaa taagacgcgt
     1381 ggggtcacta ttccaaagca agacattttc agggaagagg aaaattgcga gatggcccac
     1441 agccagcata atgattccta tgtgaaaggg tgtagtgccc cagaaaccca cattgttttc
     1501 tagaaactgt gctgataggc ttgaaatgct gtacccttct tgtgtgtagc ggtaaatagt
     1561 acccaccaag agcaccacaa ttgacacata cgggtaggca ataaataaga cagaatctag
     1621 ggtaatcatt gcattagttc tccattagtc gttcctttct taaaagcctt cgagcctgct
     1681 tccagttcat tttcttgggt gatatttatt agaaaatcac tcgtctctgg gaaggtttca
     1741 gcgataggaa aatctttttt caaaacctca aacaatgcta ttaaggggta ttggtaaagg
     1801 gtattggtag cgagcagctt cgagggtgat tcgattaaag ttttataatg tttctcatat
     1861 gcttgatgct tttgtgtgat acgaccatca tcaaactcag caatcatttg atgcaacgcg
     1921 ggggctagaa tttcatcaat catatctatg agaagatctc catcttctaa tactgctatt
     1981 agttttaata cattgggtaa atgatcagcc aactcgtgtc cacaatccac atgatgctga
     2041 atatgttctc ggttgaggtg tgatagaagc tgtcctctct tataatcatc tccaaaaaga
     2101 acataaccaa tatccaaggt tgttatagcc tgcacatcaa aagatcgggt atagatttct
     2161 tgcatcattc gcagatcatg ttctggtaaa agtttgatga attgatcaag tgaaaagatg
     2221 gctccaggat aggcatcttc ta
//
LOCUS       DASCTH010000168         2461 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000256734, whole genome shotgun sequence.
ACCESSION   DASCTH010000168 DASCTH010000000
VERSION     DASCTH010000168.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000256734"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="QGH35_03565"
     CDS             complement(<1..432)
                     /locus_tag="QGH35_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HJL81644.1"
                     /translation="MKSVKEIELEIVKEFEIFDSWMDRYEYIIDLGKQLPVFPDQWKV
                     DQHRVFGCQSQVWFKTALQDNLFICQAISDSAIVSGLIALLLRIYNEQDPVDIVHTKP
                     SFISMIGLDEHLSPTRNNGLNIMLERIKNDANNMVASQKIKT"
     gene            complement(455..1825)
                     /gene="cysS"
                     /locus_tag="QGH35_03570"
     CDS             complement(455..1825)
                     /gene="cysS"
                     /locus_tag="QGH35_03570"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HJL81645.1"
                     /translation="MAIKLYNTLSRNKEAFSPLNPSHVSMYVCGPTVYSCPHIGNARG
                     PVIFDVLANLLRLDYKLTYVRNVTDLDDKIYQAAKEESAEIKTITERYTKIYHEDIQA
                     LGVTEPDIEPKATDHIPQMIVMIEKLLQGGFAYENEGHVLFRIDSFSGYGQLSNRQQE
                     DMITSSRVEIATYKENQNDFVLWKPSTADLPGWDSPWSFGRPGWHLECSSMVESYLGK
                     SIDIHGGGGDLIFPHHENEMAQSTCAHNGQKFCNYWIHHGLVNFKHAKMSKSEGNILL
                     VRKLLENTPGEVIRLALITTHYRQPISWDDDVLNESKKKLDRLYGALRSVTDQIEEGE
                     PSEKVIEALSDDLNTPKALAELFNIAKAINSTKDKGEAISLSASLKSSAKLMGLLQAD
                     PDEWFKTSHKDSLTAEEIETLIKQREAARSSSNFVEADEIRDRLLSLGVVIEDGDEGT
                     RWKYLD"
     gene            1895..2380
                     /gene="hisI"
                     /locus_tag="QGH35_03575"
     CDS             1895..2380
                     /gene="hisI"
                     /locus_tag="QGH35_03575"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HJL81646.1"
                     /translation="MKTVNIFSERTSIKQVEEGNELAPKFNSKGLIPVVTTDAASGEL
                     LMHGFMNQEALKLSIETGEMHYYSRSRQKIWLKGETSGFKQTIKELLIDDDQDALWAK
                     VTATGGASCHVGYRSCFYRRINLDRDINSDQKAKLRFTETKKVFEPTEVYPEESNPTK
                     L"
BASE COUNT          753 a          503 c          436 g          769 t
ORIGIN      
        1 agttttaatc ttttggctag caaccatgtt atttgcgtca ttcttgattc tttccagcat
       61 gatatttaat ccattgttcc gggtgggact taagtgttca tcaagaccaa tcatcgatat
      121 aaaagatggc ttggtatgaa caatatcaac gggatcttgc tcattgtata tcctcagtag
      181 cagggctatt agccctgaga ctatagcaga atcactaatg gcctgacata tgaataaatt
      241 atcttgcaac gctgttttaa accacacttg ggattgacag ccaaaaactc tatgttgatc
      301 gactttccat tgatctggga atacaggtag ctgttttcca agatcgataa tatattcata
      361 gcggtccatc cagctgtcaa aaatctcaaa ctctttaaca atttctaatt caatttcttt
      421 aactgacttc aatggttata accttaaccc tgttctagtc caaatacttc catcgcgtcc
      481 cctcatctcc atcttcaata accactccca aactcagcaa tctatctcta atttcatctg
      541 cttcaacaaa attacttgat gatctagctg cctctctttg tttaattaat gtttcaattt
      601 cctcagcagt cagagagtcc ttatggctag ttttaaacca ttcatccgga tctgcttgca
      661 gcaatcccat taattttgca gatgacttta aacttgcgga caaagagatt gcttctcctt
      721 tatccttggt gctattgata gctttagcga tattaaaaag ttcagccaaa gccttggggg
      781 tattgagatc gtcactgagt gcctctatta ctttttcaga cggctcgcct tcttcaattt
      841 gatcagtaac agacctaagt gctccatata atcgatccaa tttcttcttt gattcattca
      901 gcacatcatc gtcccaacta attggctgtc tatagtgagt agtgataagt gccaatcgaa
      961 ttacttcacc aggagtattt tcaagaagtt ttctcaccag gagaatatta ccctctgatt
     1021 ttgacatttt tgcgtgttta aaattgacca aaccgtgatg aatccagtaa ttacaaaatt
     1081 tttgcccatt gtgtgcacag gtactctgtg ccatttcatt ctcatggtgt ggaaaaatta
     1141 aatcacctcc tcctccgtga atgtctatac ttttccccag gtaactctct accatagagg
     1201 agcactctaa atgccaacca ggtctcccaa aactccaagg gctatcccat cctggcaaat
     1261 ctgctgtaga cggcttccaa agaacaaaat cattctgatt ttctttgtaa gtagcaattt
     1321 ccactcttga actggtgatc atgtcttctt gttgtcgatt agacaactga ccatagccag
     1381 aaaatgagtc aattctaaac agtacgtgcc cttcattttc ataagcaaat ccaccttgta
     1441 agagcttttc aatcattaca atcatctgag gaatatgatc tgtagcttta ggctcaatat
     1501 caggttctgt aacacctagg gcttgtatgt cttcatggta aatttttgta taacgttctg
     1561 taatagtttt gatttcagca ctctcttctt tcgcggcctg ataaatttta tcgtcaagat
     1621 cagtaacatt tcgaacatag gttaacttgt aatctaacct cagcagattt gccaatacat
     1681 caaagataac tgggcctcta gcattgccga tatgaggaca actatagacg gtgggtccgc
     1741 aaacatacat ggaaacatgg ctgggattaa gtgggctaaa tgcctccttg ttacgggata
     1801 aagtattgta tagttttatt gccataaaat agtctaattg ctctttccat agttataata
     1861 cctaatgtta aaagaagata ccgaggatca ttaagtgaaa actgtaaata ttttctctga
     1921 aagaaccagc ataaagcaag ttgaagaagg taatgagtta gcaccaaaat ttaactctaa
     1981 aggtctcatc ccagttgtga ctaccgatgc agcatctgga gaattattaa tgcatggatt
     2041 tatgaatcaa gaagcattga aacttagtat agaaactgga gaaatgcatt attacagcag
     2101 gagcaggcag aaaatctggc tcaaaggtga aaccagtggt tttaaacaaa caataaagga
     2161 attgttaatc gatgatgatc aagacgcatt atgggctaaa gttacagcaa ctggaggagc
     2221 cagttgtcat gtaggctacc gttcctgttt ctatcgaaga attaatctag acagagacat
     2281 aaatagtgat caaaaggcta aactgcggtt cactgaaaca aaaaaagtgt ttgaaccgac
     2341 tgaagtctac cctgaggaat caaatccgac taaactttga agagcataga tgtcagattt
     2401 aattgaaatc attagctttg atctctggga taaagatcca gaacaatttg ctgagaaact
     2461 a
//
LOCUS       DASCTH010000169         2543 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000258991, whole genome shotgun sequence.
ACCESSION   DASCTH010000169 DASCTH010000000
VERSION     DASCTH010000169.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000258991"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="QGH35_03580"
     CDS             <1..559
                     /locus_tag="QGH35_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019284.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81647.1"
                     /translation="MQIQYGLLLKYNSLMKYLILILPLFLVAFEQPPIDASKLIEKNG
                     IYYEADPLKVFTGTLLHYYEDGRVKERAGYQNGTREGFRETFHENGRLFEKENYKNGE
                     LSDLFQGFYDNGQLWWQGQAKNNLRNGQLQSFYANGQLKYQGQYKDNSQEGVWQYFDK
                     DGSLSEIRCYVNNAQVELSGCSRPK"
     gene            639..2348
                     /locus_tag="QGH35_03585"
     CDS             639..2348
                     /locus_tag="QGH35_03585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020029990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81648.1"
                     /translation="MKTLLILTILFFLPISVNSDELLLEKIYLAREVITLDDVHPEAT
                     AVLVRGDRVAAIGGVEQLQSNFPNVELDRRFAENIMVPGLIEHHVHPLLAAITMGVDV
                     IAIEDWALPGKKILGVRDRQGYIDRLQAAEQQQLTADTPLVTWGFHHYFHDKLTRQDL
                     DSISNSRPILVIHRSFHEFIMNTPALTYFGITPELIENLDAEVKQYANFENGHFSEQG
                     AVSVLPNLMQYLAAPERLIAGLQMTEKYLHQNGITLVANPGAMLQQSIQAAKNYVLGD
                     LDTPFRTYFIPSGMFLAENYEPDALVTEARKLLSWGSGKLQYLPGHIKLFTDGAMYSQ
                     NMMMRDGYLDGHQGAWLMQEKTYREAFKQYWDADYQIHIHQNGDIGLDRLLDVLEENL
                     NRKPRIDHRTVIVHFGYSQPDQVARIKKLGAIVSANPYYVTALSDLYSRRGIGAARSQ
                     QMVRLGDLQRAGIPISLHSDMPMAPGAPLQLMHSAVNRVNFANEVAGPEQRISPEQAL
                     RAVTINAAFTLGLEKDYGSISIGKYANFTLLTENPLTIDPLKINEIEIRGTMVEGRHF
                     PIN"
BASE COUNT          769 a          519 c          542 g          713 t
ORIGIN      
        1 catgcaaatc caatacggtc ttttattaaa gtacaattcc ttaatgaaat atctaattct
       61 aatactccca ctatttctgg ttgcttttga gcagccacca atcgatgcta gcaaactcat
      121 tgaaaaaaat ggcatctact atgaagctga ccccttaaaa gtctttacag gaactctgct
      181 tcattattat gaagacggta gagtgaaaga gagagcaggt taccaaaatg gtacaagaga
      241 aggctttaga gaaacattcc atgaaaatgg tcggttattt gaaaaggaaa attacaaaaa
      301 cggcgagtta agcgatttgt ttcaaggttt ctatgacaac ggacaacttt ggtggcaggg
      361 acaggctaaa aacaatttac gtaacggaca acttcaatct ttctatgcca atggccaact
      421 gaaatatcaa gggcagtata aagataattc ccaggaggga gtctggcaat actttgataa
      481 agacgggagc ttaagtgaaa ttagatgcta tgtaaacaat gctcaggtgg aactctccgg
      541 ctgcagcaga cctaagtgag tttgatattt aaaacctgac tacgctcaag aaaacataaa
      601 ttcttgctgg tgaaaggtgt gagtgtaaaa tatgtgatat gaagacactc ctaatactaa
      661 ctatcctttt tttcttacca atatctgtaa atagtgatga attactactg gagaagatct
      721 atcttgccag agaagtaatc actctcgatg atgtccatcc tgaggcaact gcagtactgg
      781 tacgaggtga ccgagttgct gctataggtg gagttgagca actgcaaagc aattttccca
      841 acgtagaact agatcgtcgt tttgctgaaa atataatggt gcctggtctg attgaacatc
      901 atgtgcatcc cttattagcc gcaatcacta tgggagttga tgtgattgca attgaggatt
      961 gggcactgcc aggaaaaaag atccttggtg ttcgtgaccg gcagggttac attgaccgtt
     1021 tacaagccgc agaacagcag caattaactg ctgatactcc tcttgttaca tggggttttc
     1081 accattattt tcatgacaag ctgacgcgtc aggatcttga ttcaatttcc aactcacgtc
     1141 caatcctggt gattcatcgt tccttccatg agtttattat gaacactcca gctttaacct
     1201 attttggtat tacgccagaa ttgattgaga atctggatgc agaagtaaaa cagtatgcca
     1261 attttgaaaa tggacatttt tcagaacagg gagcagtctc agtattaccc aatttgatgc
     1321 aatatttagc cgctccagaa cgtttaattg ctggtcttca gatgacagag aaatatctcc
     1381 accaaaatgg cataaccctg gttgccaatc caggtgcgat gctgcagcaa tcgatacagg
     1441 cggctaaaaa ttacgttctc ggcgatctgg atacaccctt tagaacttat tttattccta
     1501 gcggaatgtt tctggctgaa aactatgaac cagatgcctt agtcactgag gccagaaaac
     1561 tactgtcttg gggaagtggc aaacttcaat atcttcctgg acatattaaa ttatttaccg
     1621 atggcgccat gtattcacaa aacatgatga tgagagatgg ttatcttgac ggacatcaag
     1681 gagcatggct gatgcaggaa aaaacctacc gtgaagcttt taagcagtat tgggatgctg
     1741 attatcagat acatatccat caaaatggtg acattggatt ggaccgttta ctcgacgtgt
     1801 tggaagaaaa ccttaaccga aagccacgta ttgatcatcg gactgtcatt gtccatttcg
     1861 gctattccca acccgaccag gtagccagaa tcaaaaaact gggagcaatt gtcagtgcta
     1921 atccttatta tgtgacagca ttatctgatc tttatagtcg gcgtggcatt ggagcggcta
     1981 gatcacaaca gatggtaaga ctgggcgacc ttcaacgcgc cggtattcct atatccctac
     2041 attcagatat gcccatggca cccggggcac ctctacaact gatgcattca gcagttaacc
     2101 gggttaattt tgccaatgag gtcgctggac ccgaacaacg catctctcct gaacaggcac
     2161 tacgtgcggt gaccattaat gctgctttca cgttgggatt ggaaaaagat tatgggtcga
     2221 tcagtattgg aaaatacgca aactttactc tattaactga gaacccgctg actatagatc
     2281 ctttgaagat caatgaaata gaaatacgag ggacaatggt tgagggacga cattttccaa
     2341 taaactaatc agctctcttg gggactttaa atacttaaac ggtccaagac catagggaaa
     2401 gcctcgacgt atctttgtac atcctcgatc ttgcccatgc tgactcttga ccagttcgta
     2461 tgttttccat atttgccggc aatgataatg ttttgctttc tcatttcatc acgaaaagtg
     2521 tcagcattaa tgttgccaag att
//
LOCUS       DASCTH010000170         1911 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000267658, whole genome shotgun sequence.
ACCESSION   DASCTH010000170 DASCTH010000000
VERSION     DASCTH010000170.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000267658"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(42..1382)
                     /locus_tag="QGH35_03590"
     CDS             complement(42..1382)
                     /locus_tag="QGH35_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HJL81649.1"
                     /translation="MDSTHLIEMNQGMMVSGIILALSFIGIFTETLHGFSRVKVAMLG
                     ALVMLVVGQSYGFYNPELAFEAVDWNVVFLLAAMMSIVAIMIKTGGFEVLATKIGQIA
                     KGRQFLLMAMLGTAVTVISLLLDNVTTVVIFGPLIVLICQKMKVNAIPYLMAAALLSD
                     TGGVATLVGDPPNLMIGSAANIAFTPFVEKMGLIVLVAWLGTLFFLRVLFKTDLDKKA
                     EGTFTDVIPYKDKSLWNKSLMVLALMVVLFIIHNQIHWEPWMVAVVGLIILSLMAKSL
                     EFEEVMHEVEIPLLMFFISLFMIVGGVENSEFLQYLGQFIIPFVKEDFLLACIVLMWV
                     AAIMSAAIDNIPFTAAMIPIILSLEAQGINVAALWWCLALGVGMGGNGTHIGSTANVY
                     IVTVSERLARREGNPDLAITPLLWAKKGLPVMILTLLICTAIMYLFFDYYSAPI"
     gene            complement(1462..>1911)
                     /locus_tag="QGH35_03595"
     CDS             complement(1462..>1911)
                     /locus_tag="QGH35_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HJL81650.1"
                     /translation="QDTELLIHNAPFDTAFINHEFSLAGYEKKIEDLCEIKDTLLIAR
                     KTHPGKRNSLDALCARYNVDTSNRELHGALIDAKLLGQVYLLMTGGQVGFFGDEQNLN
                     EQQSNHSLDIDYSERKIMTVELTNEDLEEHQQYLEDLLIDSDQSITW"
BASE COUNT          620 a          433 c          323 g          535 t
ORIGIN      
        1 acaactctcc agcctttatt tttctcttgc ataactaacc tctatattgg tgcactgtag
       61 tagtcgaaaa ataagtacat aattgcagta catattagta gagtaagaat catgactgga
      121 agacctttct ttgcccataa tagcggtgtt attgctaaat ctgggttgcc ttcccgcctt
      181 gccaatctct ccgataccgt cactatatat acattggcag tcgagccaat atgagtacca
      241 ttgcctccca taccaacccc tagtgcaaga caccaccata gggcggcaac attgatgccc
      301 tgagcttcca gagaaagaat gattgggatc atggctgcgg taaatgggat gttgtcaata
      361 gccgcagaca ttatggctgc aacccacatc aagacaatac aagctagcaa aaagtcctct
      421 ttgacaaaag gaatgataaa ttgaccaaga tactgaagaa attcactgtt ctcaacacct
      481 ccaacaatca taaacaacga aataaaaaac atcagcagcg gaatctcaac ttcatgcatc
      541 acttcttcga attcaaggct ctttgccatt aagctcaaaa tgatcagtcc cactactgcc
      601 accatccatg gctcccaatg tatttggttg tggataataa aaagcactac catcaaagcc
      661 aagaccatga gtgacttatt ccataagctt ttatctttgt atgggataac atcggtaaag
      721 gtaccttctg cttttttatc aagatcggtt ttaaacagca ctctgagaaa aaataatgtg
      781 cccaaccaag cgaccaaaac gattaggccc attttttcca caaatggagt gaaagcaata
      841 tttgctgccg aaccaatcat caaatttggc ggatcaccaa caagagtagc tacacctcct
      901 gtgtcggaca acaatgccgc tgccattaaa tatggtatgg cattgacttt cattttttga
      961 caaattagca cgattaaagg accaaaaatt acaaccgtcg ttacgttgtc taagagaagt
     1021 gaaattacgg taacggctgt gccaagcatt gccattaaca gaaattgtct acctttagca
     1081 atttgtccta ttttcgtggc tagaacttca aaaccaccgg tcttaatcat gatggctaca
     1141 attgacatca tcgctgccaa taaaaaaaca acattccaat ccaccgcctc gaaagctagt
     1201 tctgggttgt aaaaaccata agattgtcca accactagca tcactagtgc tccaagcatt
     1261 gcaactttga cacgagaaaa cccatgcaat gtttctgtaa aaattcctat aaagctcaaa
     1321 gccagaatga tgccagacac catcatgccc tgattcattt caatcaaatg tgtagaatcc
     1381 atttaattct ccgattggct gataaaaagg actatattct aatacaaaaa aacataaaga
     1441 atcactaaaa tataaaacag cttaccaagt gatcgactga tctgaatcaa tcagcaaatc
     1501 ttcaagatat tgttggtgtt cttcgagatc ctcattagtt aactcaacgg tcataatttt
     1561 tctttcacta tagtcaatat ctagagaatg attgctttgt tgttcattta gattttgctc
     1621 atcgccaaaa aatccaactt gaccgccagt catcaataaa tagacttgtc ccaataattt
     1681 tgcatcaatc aatgccccat gcagttccct gtttgatgtg tccacattgt atctagcaca
     1741 taaagcatcc aagctgttgc gtttcccagg atgggtttta cgggcaatga gcaaagtgtc
     1801 tttgatctca caaagatcct ctatcttttt ttcataacct gccaatgaga attcatggtt
     1861 gatgaaagca gtatcaaaag gagcattatg gatcagcaac tcggtatctt g
//
LOCUS       DASCTH010000171         2151 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000269050, whole genome shotgun sequence.
ACCESSION   DASCTH010000171 DASCTH010000000
VERSION     DASCTH010000171.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000269050"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="QGH35_03600"
     CDS             <1..801
                     /locus_tag="QGH35_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HJL81651.1"
                     /translation="IFFTQVFHHNLFHADMHPGNVFVDVSNPNQPKYVAVDFGIVGSL
                     NERDLRYLARNFSAFFEQDYKKIAQCFIEAGWVPRKTRIDEFEASIRTVCDPIFDRPL
                     KDISFGNLLFRLLQTARQFEMENQPQLVQLEKTLLNIEGLGRQLYPELDLMSAAKPVL
                     DDWRKKQLDIKGLVKKLRKDAPELRETLERLPEVARNLLEERPNHNQSALTNTDIITT
                     LKRLYLGMIGMGFFIGGILLLAIKEISFWPGWVMVAISAIIILTTKPK"
     gene            825..1943
                     /locus_tag="QGH35_03605"
     CDS             825..1943
                     /locus_tag="QGH35_03605"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HJL81652.1"
                     /translation="MKALLTLDSVLKTINQPLGEEDFLEPLELFLNDLKNEASLNTFG
                     SIGIRQNIINKLQTRAKLYALVNQESLPPPSMPLVITGLPRSGTTFLLDLLHCDTRYR
                     GPLAWEIFYPLPLATTKRQTRARIRRTNFDFRMIKVAVPNFDSIHPLHAEHPEECQLI
                     TSLDLRSIHFAYMARVPNYENYIKTCNFSSAMLWHSRFLSALERNNKPDTWLLKDPWH
                     IQHIPEFLSAYPKACFVFTHRNPIEAIPSISSLSAGIRFGLSNHVDKNEIGLNSVSFW
                     SHAMEKYLMDRSQIPENQIFDINFTDLVSDPMRQMRLMYSHFGLELTEENQDHMDRFL
                     TESVTNKTPPHKYTLEEFGLTEKQVRERFKEYITEFDL"
BASE COUNT          710 a          403 c          419 g          619 t
ORIGIN      
        1 atttttttca ctcaggtttt tcatcacaac ctatttcatg cagacatgca cccaggtaat
       61 gtttttgtgg atgtttctaa tccaaatcag cctaaatatg tagcagtaga ttttgggatt
      121 gtaggcagtc ttaatgaaag agacttgcgt tatcttgcta gaaatttttc agctttcttt
      181 gaacaagatt ataaaaaaat agcgcagtgc tttattgaag caggatgggt gcctagaaag
      241 acgcgaatcg atgaatttga agcctctatt agaacagttt gtgatccaat ttttgatcga
      301 ccacttaaag atatttcctt tggaaatctg ttgttcagac tgttgcaaac tgcaagacaa
      361 tttgaaatgg aaaaccaacc acaactggtt caattggaaa aaacattatt gaacattgaa
      421 gggttgggcc ggcaactgta tccggagtta gatttaatgt cggcagccaa gccagtgttg
      481 gatgattgga gaaaaaaaca actggatatt aaaggattag tcaaaaagct tcgaaaagat
      541 gcgcctgaac taagagagac tctggaacgc ttgcctgaag ttgcaagaaa tctactcgaa
      601 gaacgaccca atcacaatca atcagccctc accaatacgg atattatcac gacattgaaa
      661 agactttatc ttggaatgat agggatggga ttcttcattg gaggtattct gttattggca
      721 attaaggaga tttctttttg gcctggatgg gtaatggttg caataagcgc tatcataatc
      781 ttgacaacaa aacctaaata atttgacaag gagtaaattc acaattgaag gctttgttaa
      841 cacttgactc tgttttaaaa accataaacc aacccttggg tgaagaagat tttcttgagc
      901 ctttggagct ttttctcaat gatttaaaaa atgaagctag cctcaataca tttgggtcta
      961 ttggcattag acaaaatatc atcaacaaac tccaaacaag agcgaaattg tacgcattgg
     1021 tcaaccaaga atccttacct cctccatcaa tgcctttggt tataaccgga ctcccaagat
     1081 ctggaaccac ctttttattg gatttattgc attgtgacac cagatataga gggccattgg
     1141 cttgggaaat tttttacccg ctgccactag caacaacaaa aaggcaaacc agagcaagga
     1201 tcagaagaac gaattttgat ttcagaatga tcaaagtggc agttcctaat tttgacagta
     1261 tacatcctct gcatgcagaa catcctgaag agtgtcaact aattacaagt cttgacttaa
     1321 gaagtattca cttcgcgtat atggcaagag tgcccaacta tgaaaattac attaaaacat
     1381 gtaatttttc atcagccatg ttgtggcatt caagattttt gagcgcgctt gaaaggaata
     1441 ataaaccgga cacatggcta cttaaagatc cgtggcatat tcaacatatt ccggaatttc
     1501 tttctgccta cccaaaagca tgttttgttt ttacccatag aaatccaatc gaggctatcc
     1561 cttcgattag tagtttgtct gccggtatcc gtttcggttt atcaaaccat gtggacaaaa
     1621 atgaaattgg tctcaattca gtttcttttt ggagtcatgc aatggaaaaa tacctgatgg
     1681 atagatccca aatacctgaa aatcagatat tcgatattaa ttttactgat ctagtctcag
     1741 acccaatgag acaaatgaga ctgatgtatt cacattttgg tttggagctc actgaagaaa
     1801 atcaagacca tatggatcgt ttcttaaccg aatctgtaac aaacaaaaca cccccccata
     1861 aatacacttt agaagaattt ggactcacag aaaaacaagt cagggagaga tttaaagaat
     1921 atatcactga atttgatctt tgaaaaacaa ggtttgagca atcattcaat aaggagaaaa
     1981 ttatgtttcc aacaatgcgc aggatcgtta ccggtcacaa tgaaaaaggc aagtcggtag
     2041 ttatcattga tagcccgcct ccacaaacac ttggccgtga agaaggcggt ttgtttgaaa
     2101 tttggaatac agacggggat cctattgata ctaaggatcc cacagaccgg g
//
LOCUS       DASCTH010000172         9761 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000270629, whole genome shotgun sequence.
ACCESSION   DASCTH010000172 DASCTH010000000
VERSION     DASCTH010000172.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000270629"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="QGH35_03610"
     CDS             complement(<1..1228)
                     /locus_tag="QGH35_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HJL81653.1"
                     /translation="MTRNLSKNLQISLVMISFLFVISCSWMGDRNYEATIRVTSYGIP
                     HILAEDWGDLGFGYGYQFTSDNFCELAKHVVRVNGEMSKYFGRSNQNLASDALIKFFG
                     KKSTIRVNGLLHADKRMVEVSEGYAAGYNHYLDNIPQGMHQSCINAEWLRPIDLYDVA
                     RMSVYITLLASFSDPRIANAVLALGIEEGNEQTALNLETFALSESMGSNSYALGSKVT
                     QTGQAMLLGNPHYPWRGQRRFYQVHMTIPEEIDVMGISILGSPLINVGFTEKFAWTHT
                     VSNANRFTLYELDLNDQDRKIYTYNQKRTNIKSFPIEIEVKESDDSISTETIELHFSR
                     FGLLLDAGVLLGDESLTGWPNRDGKVFAIRDVAMDNQRIGTTIVGMLTAKSFDQFINT
                     IRDNLGLSFINTIAVND"
     gene            complement(1346..3703)
                     /locus_tag="QGH35_03615"
     CDS             complement(1346..3703)
                     /locus_tag="QGH35_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018997393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81654.1"
                     /translation="MKLKNIFIAAFIAAGIFTYNTPWAAIEEIVVTATKRDSTIQDVP
                     FSINAQTAETIQRTGAVSFEDLARNVAGLAVQNLGPGQSQVGIRGLSAGQIVRDQPGV
                     KEQVGVYMDESVISLSLFTPDFDLYDLNRVETLRGPQGTLFGSGSIGGTVRYITNQPN
                     VDEFEAELSLDLNTITQGSNGGHIKAMVNTPLGNGGAVRAVVYSTEYGGYIDATGEGG
                     TFNADVNEGSRDGARISATFSPSDTLTITPRIIFQETEYDGYNRQEEFNLFANPFTTT
                     RTAKISKPRQQHLLQQEGYSDETTLADVVVESGFDGYDMTFVGSYMERDILVSRDATA
                     LTSSVWHDIGYDAEMYTLPSNLRDTTALEQTTAELRFASNTDGPLQWLVGFFYSDMQR
                     EYAQRLPTPGSAVWTDHTLGAGTSAAVANGYSNLDSPFNSDLPYDIQQIALFGEATYD
                     LSDQLQLTFGGRYYDLDESRVITSGGLFANGDSGTVNETTSSGFAPRVLLRYDMNDDV
                     TLNAQISQGVRLGGVNDPLNANMCTPEDKLIFGSYQSYDDETMWNYEVGIKSRLGNGA
                     TLNAAVFYADIENLQATLDAGSCSSRISFNVPDSKTSGVEVEYVSQPTDYLTFSISGS
                     AVNAEFKSTVMDATGSVLGGLQDGNRLASVPELQLAATATYVFPIVLGDAYASATVHH
                     VGDRITQPTDQLYGAGDFASGLVFGGASGADITSLDLVLPSYTIFNIRVGVEVASGWD
                     AALYINNATDENAHLSFDRERGGRARLAFRTNQPRTIGVNIRKHF"
     gene            complement(3867..5603)
                     /locus_tag="QGH35_03620"
     CDS             complement(3867..5603)
                     /locus_tag="QGH35_03620"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81655.1"
                     /translation="MQQRSKKIPIIIIIVCMLLVVFSIYLEGNKKAANIGPDLIVYNA
                     DIRTSDPESPLAQAFAVNKGKFIAIGTNQEIQSLGKKNTQHIDAQGLSVLPGFIDSHT
                     HLSSGGKIVAGINLTGVKEKSVWLDMIAEKVRTMEPGEWLLGGRWDYTLEQKGLPTRW
                     ELDEVSPHNPVALSDIDGHSMWVNTLAIEKANITANSVVPVGGQIMLDEDTGKPNGIL
                     LESAMDLVWRDETYVRDSDKARKQISQVLNFATSLGITSVHDMSSKIELEKYLELAEE
                     GKLYVRTFYGEHSKFDAGQDSTSDKAQIKKLRNKYKFNDPNKGPMVEFGFIKYVMDGV
                     LSTHTAAMLEPYSDRPDLFGEPFYTQEEINALVKRANGLGFPVAIHAIGDRAVRMALN
                     AFETSGNREYANRIEHIEIIDPNDLPRFKALNVTASMQPNHGTGVIGKYITERVGEER
                     EKNAYVWGDFLRSGTQLAFSSDFATSPFSPLAHIADAVFRESPSGLFDGTWYPDQTVT
                     FEQALSAYTQSGANLSGWGEELGSISVGKWADFVLLDSTLGNPVSRELKEAKVSATYF
                     AGILVFSVTEKS"
     gene            complement(5709..8879)
                     /locus_tag="QGH35_03625"
     CDS             complement(5709..8879)
                     /locus_tag="QGH35_03625"
                     /inference="COORDINATES: protein motif:HMM:NF027232.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HJL81656.1"
                     /translation="MNHNQGNYPMRSMRITTKVVMVISLFLGSSTCFATDTVDPELLS
                     AIEWRLVGPHRGGRVTTVAGVPDNPQLYYMGATGGGVWKTVNAGLTWENISDGFFNVG
                     TIGAIAVAESDHNVIYVGTGEKSIRGVTTSQGDGIYKSTDAGATWVHIGLQKAGQISR
                     IKIHPTNPDIAYAAAQGQIWGPNKERGVYRTIDGGKTWEQVLSVDAQTGATDLRMDPT
                     NPRILYAAMWEHGRKPWYVLSGGTAGGIFKSTDAGDNWEKLTNGLPELIGKIGVDVSA
                     SNPNRLYAIVEAMPGEGGLYRSDDGGESWELKNKSRILWTRSWYYMHIAADPVNEDIV
                     YVLNAPFMKSIDGGVNFEKKSTPHGDHHDHWINPYNNKNMINGNDGGATITFDGGESW
                     SSIMNQPTAQFYRINTDNQFPYRIYAGQQDNSTVAILSETYGNSIGNDDFYPVGGGES
                     AHIAFDPDNPRLIYATSINSTLTEYDADNERIREIKPYPEFIFGMESKDLRYRTNWNA
                     PVTASPQDPSVIYYGTEKLLKTTDRGVTFEEISPDLTKNEKDKQGLNGGPITAENVGA
                     EFYGTILTITASPHEYGTVWVGSDDGLIHVTRDDGESWDEVTPQDLRGAMINSIEVSP
                     HNPGTAYVAVAGYKMNDFRPYIYKLTDFGRQSRRIDRGLPDDNFIRVVREDPERQGLL
                     FAGSEVSLYVSFDDGDNWQSLALNLPPVPITDLAIRQNDLVASTQGRALWVLDDLSAL
                     RQVQNDMIGKPLHVFTPGPVEMIKGSNTSEPSKTKGTNPLRGVVFNYHIRDEQEDPLT
                     IKITDNNGNLVRTYSSQESDFERCIIGNMDQRQPFKVKYPPKEQGANKWVWDMRRNGL
                     HCIDDIKLFEGFAGAYVMPGTYKALISIGDAEDTVTLTLITDRRITADSYAIAEVQQK
                     VSEITALMNELLDSLTSIRKSRTQIKALMTDFPDAELLQDSGQSAIDRLSTWEREVLQ
                     VDYETYEDEDNLPGKLVKQVRHLLDVIDDAGPPIATGALDRLRDLQDAWTNLQAELRD
                     ISTSDIEAVNNWASENTIPHISPPIT"
     gene            complement(8910..9668)
                     /locus_tag="QGH35_03630"
     CDS             complement(8910..9668)
                     /locus_tag="QGH35_03630"
                     /inference="COORDINATES: protein motif:HMM:NF039614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HJL81657.1"
                     /translation="MTREINAAMGSCELTFLLRDKIDMDLAKQQHQQYQSVLSSLGCE
                     IVIVSTESNLADSVFIEDTAVVLDEIAVLCRPATALRGQEVAGVKDVLQQYRTLASIQ
                     SPGTLEGGDLLRVGNIIYAGLSTRSNQSGIEQLRYIVADYGFSVESVETKKCLHLKSA
                     VSEVAPGVLLINSDWISRSVFKNYELIDVDKEEAHAANALLVDQKVIYPSSFPRTLEK
                     LVNAGLDVTQVNVSELQKAEGAVTCCSLIFKMSL"
BASE COUNT         2785 a         2231 c         1882 g         2863 t
ORIGIN      
        1 gatcgtttac tgcaatggtg ttaataaaac ttaatcctaa attatctcta attgtgttaa
       61 taaattgatc gaagctcttc gccgttaaca tgccaacaat agtggttcct attctttggt
      121 tatccatggc aacatcacga attgcaaaaa ctttgccatc tcggtttggc caaccggtca
      181 aactctcatc gcctaacaac actcctgcat caagaagcaa accaaagcga ctgaaatgta
      241 attctattgt ttctgtagat atcgaatcat cgctctcttt aacttctatc tcaatcggga
      301 aagattttat gttggttctt ttttgattat aggtatagat ttttcgatct tggtcgttca
      361 aatccagctc gtataaagtg aatcgatttg cattggacac cgtatgggtc caagcgaatt
      421 tctcggtaaa accaacattg atcaaggggc ttcctagaat agagatcccc ataacatcaa
      481 tctcttcagg aatagtcata tgcacctgat aaaaccgtct ttgccctctc caagggtaat
      541 gtggattccc taatagcatc gcttgacctg tttgagtgac tttactaccc aatgcgtaag
      601 agttgctacc cattgattca gacaatgcaa acgtttctag attcaaggct gtttgttcat
      661 taccttcttc tatacctaat gcaagaaccg cattagcaat ccgaggatct gagaaagaag
      721 ctagcagcgt aatgtagaca gacattctag caacatcata gagatcaata ggtcttaacc
      781 attcagcatt aatacacgat tgatgcatcc cttgaggaat attgtcaaga tagtgattat
      841 atcctgcagc gtagccttct gagacctcca ccattctctt atctgcgtgc agcaaaccgt
      901 taacacgaat ggttgatttt tttccaaaaa actttatcaa ggcatcacta gcaaggtttt
      961 gattggatct gccaaaatat tttgacattt caccattgac tctcacgaca tgtttggcga
     1021 gctcacaaaa attatcgctg gtaaattgat aaccatatcc aaaacctaga tctccccaat
     1081 cttctgccaa gatgtgaggt ataccatagg aggtgactcg gatagtggct tcataatttc
     1141 tatctcccat ccaactacaa gagataacaa aaagaaaaga aatcattacc aatgagattt
     1201 gtaaattctt agataaattt cgtgtcatag ttacagttta aaaactgtct ctaaagagtc
     1261 aacagtgttt tttaatttat tagaataaaa aacgcccggt tatacaccgg gcgtctttat
     1321 ttaatcacta ccttataacc aacaattaga aatgttttct aatatttaca ccaatagttc
     1381 gaggttgatt ggtcctaaaa gctagtcgag cacgaccacc acgttcacgg tcaaaggaca
     1441 agtgtgcatt ctcatcggta gcattattga tatacaatgc agcatcccaa cctgaagcta
     1501 cttcaacccc tactctgata ttaaagatcg tatatgaggg caacactaga tctaagctag
     1561 tgatgtctgc acctgatgct cctccaaata ctaatcctga agcaaagtca ccagcaccat
     1621 aaagttggtc agttggttgt gtaatacggt caccaacatg gtgaactgtt gctgaggcat
     1681 aagcatcacc aagtactatt ggaaatacat aagtggctgt tgccgccaat tgaagctctg
     1741 gcacagaagc taaacggtta ccatcttgga gtcctcccaa aactgaaccc gttgcatcca
     1801 taacagtgga tttgaattca gcatttacag cacttcctga tattgagaag gtgagatagt
     1861 cagtaggttg tgaaacatat tcaacctcaa ctccactagt tttagagtca ggaacattaa
     1921 aggaaatcct tgatgaacat gaaccggcat ccaatgtagc ttgaagattc tcgatgtctg
     1981 cataaaaaac tgcagcattc aaagtggctc cattgcctaa acgtgatttt ataccgacct
     2041 cgtaattcca catagtttca tcatcatatg actgataact gccaaagatt aatttatctt
     2101 caggagtaca catgttggca ttgagaggat cgttcacccc tccaagtctg accccttgtg
     2161 aaatctgtgc attcaatgtg acatcatcat tcatgtcgta gcgaagaaga actctgggtg
     2221 caaatccgct tgatgtggtt tcatttactg tgcctgaatc accattagca aacaatccac
     2281 ctgatgtaat gactctactc tcatcaagat cgtagtaacg tcccccaaaa gtcaattgta
     2341 actgatcact cagatcatag gttgcttctc caaacagagc aatttgctga atatcatatg
     2401 ggagatctga gttaaatggt gaatctagat ttgaataacc attagcaaca gctgcagaag
     2461 tacctgcacc taaagtatgg tctgtccaaa ctgcggaccc tggagtagga agacgctgtg
     2521 cgtactctct ctgcatatct gaataaaaga aacccactaa ccattgcaat ggtccgtctg
     2581 tatttgaagc aaaacgaagt tctgcagtag tttgctctaa tgctgtggta tccctaagat
     2641 tcgatggaag ggtatacatc tcagcatcat atcctatatc atgccataca ctactggtta
     2701 gagcagttgc atcacggcta acaagaatat cccgttccat atagctaccc acgaaagtca
     2761 tatcatagcc atcaaagcca gactcaacaa caacatctgc caaagtcgtt tcatccgagt
     2821 aaccttcctg ctgtaataaa tgttgctggc gtggcttgga tatttttgca gttctagttg
     2881 tagtgaatgg attggcaaat aagttgaatt cctcttgtcg attgtagcca tcatattcag
     2941 tctcctgaaa aataattcga ggagtaattg ttaaagtatc actcggtgag aaggtcgctg
     3001 aaatacgagc accatctcgt gatccctcgt taacgtctgc gttaaatgta ccaccttctc
     3061 cggtggcatc gatgtaaccc ccatattcag ttgaatagac aaccgctcgc acggcacctc
     3121 cattacctag gggagtattt accatagcct tgatgtgccc accatttgaa ccttgggtga
     3181 tggtgttaag atctagagac aattcagctt cgaactcatc aacattgggt tgattggtta
     3241 tgtaacgaac cgttccacca attgatccag agccaaaaag agttccttgt gggccacgta
     3301 gggtttcaac tcgattaaga tcatacagat caaaatctgg cgtaaatagg gacaatgaaa
     3361 ttacggattc atccatgtaa acaccaacct gttctttaac accaggttgg tctcgtacta
     3421 tctgtccagc agaaagacct cgaattccca cttggctttg tcctggtcct aggttttgta
     3481 ctgccaaacc agctacattt ctagctaagt cttcaaaact cacggcaccg gtacgttgga
     3541 tcgtttctgc agtttgtgca ttaatagaaa aaggcacgtc ttggatagtc gaatccctct
     3601 tcgttgcagt aactacaatt tcctcaatag ctgcccaagg agtgttgtat gtaaaaattc
     3661 ctgcagcaat gaaagctgca atgaaaatat tttttaattt cataattgtc cctctataag
     3721 tcaattgaac cagttaataa aagctaagcc taccatagtc tgtaacccac ataaaacaag
     3781 gacttggttt ttttttgaag attgttacac cttttgttgc tataaaacca ctaatggtgg
     3841 tgtttaatta agatattact tggaaattag cttttttcag tgacggagaa gaccaatatt
     3901 cctgcaaaat aagtagcgga gactttcgct tcttttaatt ctcggctaac tggattgcct
     3961 aaggtgctat caagcaagac aaaatctgcc catttaccta cactaatact gccaagttcc
     4021 tctccccatc ctgataaatt tgctccagat tgtgtataag cagataaagc ttgctcaaaa
     4081 gttactgtct gatctgggta ccaagtcccg tcaaataaac cacttgggct ttcgcgaaaa
     4141 acagcatctg cgatatgtgc aagaggagaa aatggtgagg ttgcaaaatc actactgaaa
     4201 gctagttggg ttccagatct taaaaaatct ccccaaacat aggcgttctt ttcacgctct
     4261 tcaccgactc tctcagtgat atatttccca atgactccag taccatgatt gggttgcatg
     4321 gatgctgtca cgtttaatgc tttgaatctt ggcagatcgt tgggatcaat aatttcgata
     4381 tgctcaatgc gatttgcata ctctctgtta ccagaggtct caaatgcatt taaagccatt
     4441 cttacagctc gatcaccgat cgcatgtata gctactggga aacctaaccc attagctctc
     4501 ttgacgagag cattaatttc ttcttgagta taaaaaggtt caccgaataa atcagggcga
     4561 tcagagtaag gttctagcat tgccgccgta tgggttgaga gcacaccatc cattacatat
     4621 tttataaagc caaattcaac cattggacct ttattaggat cattgaattt atatttattt
     4681 ctgagttttt taatttgagc tttatcagaa gtgctgtcct gtcctgcgtc gaacttagag
     4741 tgctctccat aaaatgtcct gacataaagt ttaccctctt ctgccaattc taaatatttt
     4801 tctaactcaa tcttgctgga catatcgtga acacttgtta ttccaagtga agtagcaaag
     4861 ttgagaactt gtgatatttg tttccttgcc ttgtcagaat ctcttacata agtttcatct
     4921 ctccaaacaa gatccatagc gctttcaagc aatattccat tgggttttcc agtgtcttca
     4981 tcaagcatta tttgtccacc gactggaaca actgaattag ctgtgatgtt ggctttctcg
     5041 atggctaaag tatttaccca catactgtgg ccatcaatat cagatagtgc cactggatta
     5101 tggggagaga cttcatctaa ttcccaacgg gtaggcaaac ccttttgttc aagagtgtaa
     5161 tcccagcgac cacctaacaa ccactcacct ggttccattg tcctgacttt ttctgcaatc
     5221 atatccaacc acaccgattt ttctttgaca ccagtcaagt taatcccggc aacaatcttt
     5281 ccacccgaag ataagtgagt gtggctgtct atgaacccag gtaaaacact cagcccttgt
     5341 gcatcaatgt gttgggtatt tttttttcct aagctctgaa tctcttgatt ggttccaata
     5401 gcaataaatt ttcctttatt gacagcaaaa gcttgagcca atggagactc cgggtctgag
     5461 gttctgatat cagcattgta aacaatcaaa tcaggtccaa tgtttgcagc ttttttatta
     5521 ccttctaaat agattgaaaa cactacaaga agcatgcaca caatgataat aatgataggg
     5581 attttctttg atctttgctg catacctaaa cgttgatcga aaataataaa aatgaatgaa
     5641 aaggatcgac tataaaatat acattgacca atgaaccagg tactttcatc tacgtgccaa
     5701 taataacgct atgtaattgg tggtgaaata tgtgggatag tattttcact ggcccaatta
     5761 ttgacagcct caatatctga ggttgaaatg tctcgtaatt cagcctgtaa attggtccat
     5821 gcatcttgaa ggtctctcag tcgatcaagt gcacctgttg cgattggtgg acctgcatcg
     5881 tcaattacat ccagtagatg acgcacctgt ttgactagct tacccggaag attatcttca
     5941 tcttcgtaag tctcgtaatc aacctgcaaa acctctcttt cccatgtaga tagacgatcg
     6001 atcgcactct ggccagaatc ctgaagcaac tctgcgtctg ggaagtcagt catcaaagcc
     6061 ttgatttggg ttcttgactt tcgaatggag gttagactgt caagcaactc gttcatcaac
     6121 gctgttatct cagacacctt ttgttggact tcggcgatag cataactatc agctgtaatt
     6181 cgacggtctg tgatgagcgt gagggtcaca gtatcttcgg catcaccaat tgaaataagt
     6241 gccttataag taccaggcat gacatatgcg ccagcaaatc cctcgaataa cttaatatca
     6301 tcaatacaat gcagaccatt cctgcgcatg tcccagaccc acttgttagc accctgctcc
     6361 tttggtggat atttgacttt aaacggttga cgctggtcca tattaccaat gatacaacgt
     6421 tcaaagtcac tttcttgact tgaataggtg cgcacgagat taccgttatt gtcagtgatc
     6481 ttaatggtta acggatcttc ttgttcatca cgaatgtggt agttgaatac aacgccacga
     6541 agggggttcg tgcccttggt cttggagggt tcagatgtat tactaccctt aatcatttca
     6601 accggtccgg gggtaaaaac atgtagtggc ttgcctatca tgtcattttg cacttgacgc
     6661 agagcagaca ggtcgtcaag cacccagagg gccctgcctt gcgtagaggc aacaaggtcg
     6721 ttttgacgaa ttgctagatc agtaattggt accggcggca ggttgagagc cagtgattgc
     6781 cagttgtcac catcatcaaa agacacatag agactaactt cgctaccagc aaataggagt
     6841 ccctgccgtt caggatcttc acgaacgaca cgaatgaagt tatcatctgg caaacctcta
     6901 tcaattcttc tcgactgtcg tccgaagtct gttagcttat agatatatgg tctgaaatca
     6961 ttcatcttat agccagcgac agcaacgtaa gccgtgccgg gattgtgtgg actcacttcg
     7021 atagagttaa tcatggcacc acgtagatct tgaggtgtga cttcgtccca gctttcgcca
     7081 tcatcacggg tgacatggat taggccatca tcactaccaa cccaaaccgt gccatattca
     7141 tgtggcgatg cagtgatggt cagaatagta ccataaaatt ctgcaccgac attctcggca
     7201 gtaataggtc caccattcag accttgttta tctttttcat tcttagtcaa atccggacta
     7261 atttcctcaa acgtgactcc acggtcagta gtctttaata gcttttctgt accgtaatag
     7321 atgacgctgg gatcctgtgg tgatgcagta acaggagcat tccaattggt acggtagcgc
     7381 agatcctttg actccattcc aaatataaat tccggataag gcttaatttc cctaatgcgt
     7441 tcattatcgg catcatattc agtgagtgtg ctgttgatac tagtggcata gatgaggcgt
     7501 ggattgtcag gatcaaaagc aatatgagca ctctcgccac cccctaccgg ataaaagtca
     7561 tcattaccta tactgttgcc atacgtttca ctaagaatgg cgacagtact gttgtcttgt
     7621 tgccctgcat aaattcggta cgggaactga ttgtctgtat tgattcggta aaactgtgct
     7681 gttggttggt tcataatact cgaccaggat tcaccaccat cgaaggtaat agtggcacca
     7741 ccatcattac cattgatcat gttcttattg ttataaggat taatccagtg atcatgatga
     7801 tcaccatgtg gtgtagattt cttttcaaag ttcacaccgc catcaattga tttcatgaat
     7861 ggtgcattga gtacgtagac aatatcttcg ttcactgggt cagctgcgat atgcatatag
     7921 taccaggagc gggtccaaag aatacgcgac ttgtttttca attcccaaga ctccccacca
     7981 tcatcactac ggtacaagcc accctcaccg ggcatggctt caacgattgc atacaatcta
     8041 ttcgggttgc ttgcagaaac atccactccg atcttgccaa tcagttcggg cagaccattg
     8101 gtcagtttct cccagttatc acccgcatct gtcgacttaa agattccccc ggcagtacca
     8161 ccagataaga cataccaagg cttgcggcca tgctcccaca tggctgcata gagaatcctt
     8221 ggattggtcg gatccattct aagatcagtc gcaccagttt gtgcatcaac actcaaaact
     8281 tgctcccagg tcttgccacc atcaatcgta cgatagacac cacgctcttt attgggtccc
     8341 cagatctggc cctgtgccgc agcataagca atatcaggat tcgttggatg aatcttgata
     8401 cgtgaaatct gtcctgcttt ctgcagtcca atatgcaccc aagttgcacc tgcatccgtt
     8461 gatttataga taccatctcc ctgactagtg gtcacaccac ggatagactt ttctccggtg
     8521 ccaacgtaga taacattatg atcagattct gccacagcaa tggcaccaat agtgcctaca
     8581 ttaaagaaac cgtccgagat attttcccaa gtcagaccgg cgtttaccgt cttccacaca
     8641 ccaccacctg tcgctcccat ataatatagt tgcggattgt ctggcacccc tgcgactgtt
     8701 gtcacacgac caccacgatg tggtcctacc aggcgccatt ctatggcgga aagcaattcc
     8761 ggatcaactg tatctgtggc aaaacaagtt gagctaccta agaataagct gataaccatc
     8821 actacttttg ttgttattct catcgatctc attgggtaat ttccttgatt atgattcata
     8881 tttatctaat ttacctaatc cactcactat tataaagaca tcttgaaaat caagctgcag
     8941 caagtaaccg caccttcagc tttttgcagt tcggacacgt tgacctgtgt cacatcgaga
     9001 ccagcattca ccaatttttc taaggtgcgt gggaatgaag agggataaat taccttctga
     9061 tcaacaagaa gtgcgttggc agcgtgcgct tcttccttgt caacatcaat cagttcataa
     9121 ttcttaaaaa cagatctgct aatccagtca gaattaatga gaagcacgcc gggggcaact
     9181 tctgaaacag cagatttcaa gtgtagacac ttttttgttt ctactgattc cacggaaaat
     9241 ccatagtctg caacgatgta gcgcaattgt tcgataccac tttgatttga tcgtgtggag
     9301 agaccagcat agatgatgtt accaacacga agcaggtcac caccttcgag ggtaccagga
     9361 gactggattg aggcaagtgt tcgatattgt tgcagaacgt ccttaacacc tgcaacttcc
     9421 tgtcctctta atgctgttgc cggccggcat aacactgcaa tttcatccaa aaccactgct
     9481 gtgtcttcga taaatacgga atctgctaaa tttgattcgg tcgacacaat gacgatctca
     9541 catccgagtg aagacaatac tgactgatac tgctgatgtt gttgtttcgc aagatccatg
     9601 tcaatcttgt ctctgagtaa aaaagtcaat tcacaactgc ccatggcagc atttatctca
     9661 cgtgtcatag ctgtcagcac aattttgtct cctctctatt ttttagtaaa ttattccaac
     9721 ggttctatta ataagccaga aaaaacccat ggtacctaat a
//
LOCUS       DASCTH010000173         5979 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000271059, whole genome shotgun sequence.
ACCESSION   DASCTH010000173 DASCTH010000000
VERSION     DASCTH010000173.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000271059"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /gene="priA"
                     /locus_tag="QGH35_03635"
     CDS             complement(<1..1540)
                     /gene="priA"
                     /locus_tag="QGH35_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HJL81658.1"
                     /translation="MKQAILRIALNTPVNNLFDYKPVSNKSSFEHFIGQRVRVSFGKK
                     EKIGFVHSIAKKSMVPEPKLKQVIESIDQEPIIDEELQRLIEWTSGYYHYPIGEVYAT
                     AVPLYLRQGKTPKPSPITTTSDLKSHKTLTTEQKKGFKLLGSFCENQQIVVIDGVTGS
                     GKTELYLQTIDRYIQKNKQALVLVPEIGLTPQLFEQFHQRFGNVVSVLHSDIAAKQRA
                     DIWLKAKENILKIIIGTRSGVFTPFKDLGIIIVDEEHDSSLKQQNGLRYSARDLAILR
                     AKYNNIPVILGSATPSFETLMNVKNGKYKKITLKNRVFSTPLPKINIIDLRVHPQKNG
                     LTDTLLNAIKKHTQNGYQVLLFLNRRGFSPATVCVECGHIEECPRCDTSLVLHKKNQR
                     LLCHHCGYNNPWKSLCNKCESSSIPLGQGTEQIELLLKDKFPTMKIIRIDRDTVKTKK
                     QRDLLFKEAQSGRAQIVLGTQMLTKGHDFPKLSMVAIINADQGLFGSDFRSSERFAQT
                     FIQVA"
     gene            complement(1537..2649)
                     /locus_tag="QGH35_03640"
     CDS             complement(1537..2649)
                     /locus_tag="QGH35_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJL81659.1"
                     /translation="MFIEFEMERFQSLYENEVDYNLSDSGLHPLAIKDLLNKEEINHF
                     LNMELGYGYTQGDPVLLNQVAQWYPGQSEKNILITNGSAEANFILAWSLIQPGDEIVM
                     VLPNYMQVPGLAKIFGARVKYIYLQEELDWHLNMDELQSLLTNKTKLISICNPSNPTG
                     AVMSAEELKQLATLARKYDAYIHSDEVYRGSELNGRETNSCIEFYEKSIITCGLSKSF
                     AHPGLRMGWLVADESVINDLWSRKDYSSICASVLSQYIAIKMMQPETRKKIHARATSM
                     LSTNVELFQNWLNSHPDLFSFVPPKAGGMAFVGYKMNINSTKFAHLLRTEQSVLIVPG
                     DCYGMDGYLRFGIGSPSDYLSTGLNLVSQGINNIGY"
     gene            2761..3888
                     /locus_tag="QGH35_03645"
     CDS             2761..3888
                     /locus_tag="QGH35_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HJL81660.1"
                     /translation="MNIFFPKEDNKEVRTSIVPSVVEKFVGLGATISTEKGIGETIGI
                     VDETYTKAGAVIIKDRTAALSTADIICRLNTPPIGDTSKLKKNSIHISFLDPFNEEKL
                     VNTLAKASITTISMELVPRVTRAQKMDALSSQASLGGYSAVIEAASRLAKVFPMMMTP
                     AGTVSPARVFVVGVGVAGLQAIATAKRLGARVEAFDTRPVVEEQVRSLGAKFITIDIG
                     KLEQTEQGYAKELTSEQLELQRQGMIKVCSYSDVIITTAQVFGRSAPLIITQDMINAM
                     SPGSVIIDMAVNTGGNVEGSVAGKTIISNGVQIVGIDNLPAAVAEHASQVYANNLFNM
                     IEEYWDKEKTTMDIDLTDEILSGCVITHSGKVVHSMIKQGG"
     gene            3893..4180
                     /locus_tag="QGH35_03650"
     CDS             3893..4180
                     /locus_tag="QGH35_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HJL81661.1"
                     /translation="MDSVLILLLFVLVLSIFLGFELISKVPSTLHTPLMSGANAISGI
                     TIVGAILTSRAEIFESEITFLLGMLAVFFATVNVVGGYLVTNRMLSMFKKK"
     gene            4213..5583
                     /locus_tag="QGH35_03655"
     CDS             4213..5583
                     /locus_tag="QGH35_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HJL81662.1"
                     /translation="MVNLVYILASVLFIFGLKMLGSPETAKKGNLVSAIGMLIAVVVT
                     LLDQNIVDYTWILIGITLGTIIGATAALRVKMTAMPEMVAIFNGFGGIASLLVGSSEY
                     LVSGSGFDSLIPIYLTVVIGGITFTGSLIAYSKLSGVITGNPVLYKGQQWVNAILVLI
                     LFYLGIEMVASQDWLLVEDWQVISSIVALAFLLGVLLVIPIGGADMPVVIALLNSYSG
                     LAACAAGFVIENNVLIVAGALVGASGLILTNIMCKAMNRSLANVLFSGFGSVSEGVSE
                     TGEQGEARPIISEDAYLILEAATSVAIVPGYGMAVAQAQHAVRELGELMEANGADVQY
                     VIHPVAGRMPGHMNVLLAEANVPYDKLMEPEDINPIIETIDVCIVIGANDVVNPAARE
                     DPNSPIFGMPIIDVDHAVTTFVLKRSMASGFAGIENPLFFKTNTRMLFGDAKDSITAL
                     VSEFKS"
     gene            5786..>5979
                     /locus_tag="QGH35_03660"
     CDS             5786..>5979
                     /locus_tag="QGH35_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HJL81663.1"
                     /translation="MDINQQVLRIDVTGLPLEWVDYKGAASLYAVDQVIYDLGADIYT
                     LFGGINSITGKRSQLTVNSI"
BASE COUNT         1737 a         1067 c         1223 g         1952 t
ORIGIN      
        1 cggcaacttg aataaatgtt tgagcaaacc gctcactgct tctaaagtcg cttccgaaca
       61 acccttgatc tgcattgata attgcaacca tcgataattt tggaaaatca tgacctttgg
      121 ttaacatttg agtgcctaat acgatttgtg ctcttccaga ttgtgcttct ttgaacaaaa
      181 gatccctttg tttttttgtt tttacagtat ccctgtcaat tcttataatc ttcatggttg
      241 gaaatttgtc ctttaataac agttcaattt gttccgtacc ttggcctaag ggaattgaag
      301 acgactcaca tttattacat aagcttttcc aggggttgtt gtatccacag tggtggcata
      361 ataaacgttg attttttttg tgtaaaacta gactggtgtc gcaacgtgga cattcttcaa
      421 tatgcccaca ctccacacaa actgttgctg gagaaaaccc tcttcggttg agaaacaaca
      481 gaacttgata gccattttgt gtgtgttttt taatagcatt caaaagagtg tcagtcagcc
      541 catttttttg tgggtggact cttaaatcaa tgatgtttat tttaggaaga ggggtagaaa
      601 aaacacgatt cttcagtgta atcttcttat actttccatt tttaacattc atcaatgttt
      661 caaatgaagg agttgcggaa cccaatatga caggaatatt gttatatttt gcccttaaaa
      721 tagctagatc acgggctgag tatcgaagtc cattttgttg tttaagagaa gaatcatgtt
      781 cctcatcaac aattattatt cctagatctt taaatggtgt gaatacgcct gatcttgttc
      841 ctattataat ttttaaaatg ttttctttgg ctttgagcca aatgtctgca cgttgttttg
      901 cagcaatatc agaatgcaat acacttacca cattaccaaa tctttgatga aactgttcaa
      961 acagctgggg agtgaggcct atttcaggaa caaggacaag cgcttgtttg tttttttgta
     1021 tatatctgtc aatggtttga aggtagagtt ctgttttacc gctccctgta acaccatcaa
     1081 taactacaat ttgctggttt tcacaaaatg agcccaaaag cttgaagcct tttttttgtt
     1141 ctgtggtgag tgttttgtga ctttttaagt cagatgttgt tgttattggg cttggcttgg
     1201 gggttttccc ttggcgtaga tacaaaggaa cagctgttgc atagacttcc ccaattgggt
     1261 aatgataata accagaagtc cactctatta aacgttggag ttcttcatcg ataataggtt
     1321 cttgatcaat cgactctatg acttgtttaa gttttggttc tggcaccatc gatttttttg
     1381 caatactgtg aacaaaacca attttttctt ttttgccaaa agacacccta actctttgtc
     1441 cgataaagtg ttcaaaacta gatttgttag aaacaggctt ataatcaaaa agattattta
     1501 ctggagtgtt gagagctatt ctgagtattg cttgtttcaa tagcctatgt tgtttatacc
     1561 ttggctgact aaattaagtc cagttgataa ataatcacta ggtgagccaa ttccaaaacg
     1621 caaataacca tccatgccgt aacagtcccc aggaacaatt aaaacgctct gttctgttcg
     1681 taataagtgg gcaaacttgg tgctattaat attcattttg taaccaacaa acgccattcc
     1741 cccagctttg ggaggcacga aactaaaaag gtctggatgt gaattaagcc aattttggaa
     1801 caattctaca tttgtggaca acattgatgt tgctcttgca tgtatctttt ttctagtttc
     1861 tggttgcatc attttgattg cgatgtattg agacagcaca gaggcacaaa tagacgaata
     1921 gtctttgcgt gaccacagat cattgataac agactcatca gcaaccagcc aacccattct
     1981 aagacctgga tgggcaaaag attttgataa accacaggtt atgattgatt tttcataaaa
     2041 ctcaatacaa ctatttgttt cccttccatt cagctcagag cctctataga cttcatcaga
     2101 atgaatatag gcatcgtatt ttctcgccag agtagccaat tgttttaatt cttctgccga
     2161 cattacggcc cctgtagggt tactgggatt gcagattgaa ataagtttag ttttattggt
     2221 aagtagtgat tgaagctcgt ccatgttcaa gtgccaatca agctcttctt gtaagtaaat
     2281 atatttcacg cgggcgccaa aaatctttgc taaacctggt acctgcatat aattgggtaa
     2341 gaccataact atctcatccc caggttgtat taatgaccag gccaagatga aatttgcttc
     2401 agctgagccg tttgtgatta atatattttt ttcactctgt cctgggtacc attgtgcaac
     2461 ttgattgagc aatacaggat ctccttgagt atatccgtaa ccaagttcca tgtttaggaa
     2521 gtggttgatt tcttctttat taagcaagtc tttaatagct aggggatgga ggccgctatc
     2581 acttagatta tagtcaactt cattctcata cagagactga aaacgctcca tttcaaactc
     2641 aataaacata gagaggagaa taacatattc cctcgatgtt tttcgtgtct tgacatctct
     2701 tgaaagccaa gatatcttgc cctcactaat tgctcgatat ggtttgaaat ttgataaaca
     2761 atgaatattt tttttcctaa agaagataac aaagaagtca gaacatcaat tgttccatca
     2821 gtggtagaaa aatttgtagg attgggcgca accatttcaa cagaaaaggg tatcggagaa
     2881 acaattggta ttgttgatga aacctacacc aaagctggcg ctgtgatcat taaagatcgg
     2941 actgctgcac tatcaacagc agacattatt tgtagactca atactccacc tattggagac
     3001 acctcaaaac taaagaagaa ttctatacac atcagctttt tagacccttt taacgaagag
     3061 aagctcgtca acactcttgc caaagcttct attaccacaa ttagtatgga actagttcct
     3121 agagtaacaa gagcacaaaa aatggatgct ttaagctccc aagctagttt ggggggttat
     3181 agtgcagtta tcgaagcggc cagtcgtctt gcaaaagttt ttcctatgat gatgacccca
     3241 gcaggcactg tttcacctgc tagagttttt gttgtgggtg ttggtgttgc cggtcttcaa
     3301 gctatcgcta ctgcaaaacg acttggagcg cgtgttgagg cgtttgacac tcgcccggta
     3361 gttgaagaac aagtacgttc attgggagcg aaatttatca caattgatat tggtaaatta
     3421 gaacaaaccg aacaaggcta tgccaaagaa ctaacctcag aacaactcga attgcaaaga
     3481 caaggtatga taaaagtttg tagttattct gatgtcatca taacaactgc tcaagtgttt
     3541 ggtcgttccg cgcctcttat tattacccaa gatatgatta atgcaatgag cccagggagt
     3601 gtcatcattg acatggcagt gaatactggc gggaacgttg aaggatctgt tgccggcaag
     3661 accattataa gcaatggtgt tcaaatagta ggtattgata acctacctgc tgcggtagca
     3721 gaacatgcca gccaggttta tgccaacaat ctttttaaca tgattgagga atactgggat
     3781 aaagaaaaaa caactatgga tatagattta acagatgaaa tcctatctgg gtgtgtgatt
     3841 acacattctg gcaaggttgt tcattcaatg atcaaacaag gaggttaata ctgtggattc
     3901 tgtgcttatt cttttattgt ttgttttagt gctgtcaatt tttcttgggt ttgagctcat
     3961 ttctaaagtt ccatcaaccc tacacacccc attaatgtca ggtgcgaatg ctatttctgg
     4021 gattacaatt gttggggcta ttcttacctc aagggctgaa atttttgagt ctgagataac
     4081 atttttgctt ggtatgttgg ctgttttttt tgcaacagtt aacgttgttg gtggttattt
     4141 agtgaccaac cgtatgttga gcatgtttaa aaagaagtaa taaaacgaga gagtattttt
     4201 aggaaagctt atatggttaa cttagtctac attttagcgt ctgttttgtt tatttttggc
     4261 ttaaaaatgc tgggttcccc ggaaactgcc aaaaaaggaa atttagtttc agccatagga
     4321 atgcttatcg cagtcgttgt tactttacta gaccagaata tagttgacta cacatggatt
     4381 ttaattggca ttaccttggg cactataatt ggagctacag ctgctttaag agtaaaaatg
     4441 acagcgatgc cagaaatggt agcaattttt aatgggtttg gtggtatagc aagtcttctt
     4501 gttggttctt cagagtatct agtttctggg tctggttttg atagcttaat tcctatatat
     4561 cttaccgttg ttattggtgg tattactttt acaggaagcc ttattgctta tagcaaactt
     4621 tccggtgtga ttactggtaa tcccgtgttg tacaaaggtc agcaatgggt aaatgcaata
     4681 ttagttttaa ttctgttcta ccttggtata gaaatggtag cctcgcaaga ttggttgtta
     4741 gttgaagatt ggcaagtaat ttcaagcatt gttgcactag catttttgct gggagtgctt
     4801 ttagttatac ctatcggtgg tgctgatatg ccagttgtta tagctttatt aaatagctat
     4861 tcaggactgg cagcctgtgc tgctggtttt gtaattgaaa acaatgtttt aattgttgct
     4921 ggtgctttgg ttggtgcgag cggattaatt ctaacaaata ttatgtgtaa ggctatgaat
     4981 cgctctttgg caaatgtctt atttagtggg tttggctctg tttcagaagg tgttagtgag
     5041 acaggggaac aaggcgaagc aagacctata atttctgaag atgcatattt aatattagag
     5101 gcagcaacct cagtggctat tgttccagga tatggaatgg cagttgctca agcccaacat
     5161 gctgtaagag agcttggaga actaatggag gctaatggcg ccgatgtgca atatgtcatc
     5221 caccctgtag ctggaagaat gccgggccat atgaatgtgt tgcttgctga ggctaatgtt
     5281 ccttatgata aattaatgga gccagaagac atcaacccca ttatagaaac cattgatgtt
     5341 tgtattgtga ttggagctaa tgatgttgta aatccagcag ctagagaaga ccctaacagc
     5401 ccaatatttg gcatgcctat tattgatgtt gatcacgctg taacaacctt cgttttaaaa
     5461 agatcaatgg catcagggtt tgcgggtata gagaaccctc tcttttttaa aacaaacaca
     5521 cgcatgctat ttggtgatgc taaggactct attactgcct tagtatcaga gtttaaatct
     5581 taaaccctaa acctaaacct aaacccatat ctactgaacg ctaatagaaa gcacctaaat
     5641 tcttatgtgc tgctaggcta gtggagtgct ttattgacaa ttacggcaat tttaaataaa
     5701 taattcttca gaaggtgtgt ataaatactt aataaatttt tattaaaatg aaataatacc
     5761 tagctgaagg gagtaaaaac tagctatgga tataaatcaa caggttttaa gaatagatgt
     5821 aacggggctg ccactggagt gggttgatta caaaggtgcg gccagcctct atgctgtaga
     5881 tcaagtgatt tatgacttag gagcagatat ttatactctt tttggaggga tcaactccat
     5941 aactggaaaa agaagccagc taacagtcaa ttcaatcat
//
LOCUS       DASCTH010000174         2619 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000277946, whole genome shotgun sequence.
ACCESSION   DASCTH010000174 DASCTH010000000
VERSION     DASCTH010000174.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000277946"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="QGH35_03665"
     CDS             complement(<1..617)
                     /locus_tag="QGH35_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015223377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HJL81664.1"
                     /translation="MDDSINKSNSKQNNSPTNSLPIGGMFQSRHIGLSEDDKKTMLNF
                     LGFKDMEECMQSIVPDDIYEKSSLELESPLSEEAAIARLKRLAEKNYLYKSYIGQGYY
                     NTHTPKVIQRNILENPGWYTSYTPYQPEISQGRLEALLNYQTMISDLTGLEIANASLL
                     DEATAAAEAMAMAKRVSKSSGDRFFIDEDCFSQTIAVVETRAKSLG"
     gene            complement(630..1022)
                     /gene="gcvH"
                     /locus_tag="QGH35_03670"
     CDS             complement(630..1022)
                     /gene="gcvH"
                     /locus_tag="QGH35_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007021393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HJL81665.1"
                     /translation="MNTMTGELKFSTNHEWVRLEQDNVAVVGITDFAQDLLGDIVFVE
                     LPEVGQQVQLNDEIAVVESLKAASDIYSPISGVILEINEALSDQPEVINSSPDEQGWL
                     YKIEYRNLDELNQLLSPDDYNDLCQSES"
     gene            complement(1068..2129)
                     /gene="gcvT"
                     /locus_tag="QGH35_03675"
     CDS             complement(1068..2129)
                     /gene="gcvT"
                     /locus_tag="QGH35_03675"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HJL81666.1"
                     /translation="MKKTVLNETHQALGAKMIDFSGWSMPLHYGSQITEHNAVRDSVG
                     LFDVSHMSVFDFIGKDQDAYFRRLLANDIQKINAQKKSLYSLVVNESGGIIDDLIAYK
                     LDDRYRIVSNSTTMAKNKAWFKQNIKSFDVGIKRREDLSILALQGPQAVESFNQLGIL
                     EPITFEYMGYASDQSIMVSRSGYTGEDGLELILPDHAVAETWQRLIAMNIQPIGLGAR
                     DSLRIEAGFNLYGVDMDETNHPFESNLGWSIDFRDPERNFIGKDALLNIDAKSCRQLL
                     GVIVKGKGMLRTGQKIKYPNGSGEVLCGTFSPLINASIGYARMDRGYAHNGLVSIRDK
                     HIEVEFVALPFIRNGRVVF"
     gene            complement(2228..>2619)
                     /locus_tag="QGH35_03680"
     CDS             complement(2228..>2619)
                     /locus_tag="QGH35_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HJL81667.1"
                     /translation="IDWQGIDLAQGIASKALEVARDQSVIGSSLDAHIVFYAEGEMAA
                     LLSSFGEELHFLFITSEATVESMDNLPADAIQGDGIAARVVQSEHQKCIRCWHRDPLV
                     SQSKDHPELCPRCIGNIHGDGETRLYF"
BASE COUNT          745 a          590 c          486 g          798 t
ORIGIN      
        1 cctaaacttt tggccctggt ctcaaccact gcaatggttt gactgaaaca atcttcatca
       61 ataaagaatc gatcaccgct tgacttggat actctttttg ccatagccat ggcctcggct
      121 gccgctgtgg cttcatcaag caatgaggca ttggctatct caagaccggt tagatccgat
      181 atcatggtct gataatttag taaagcttcg agtcttccct gagaaatttc aggttgatag
      241 ggagtgtagg aggtatacca tcctggattc tccaggatat ttctttggat cactttggga
      301 gtgtgggtat tgtaataacc ttgcccaata tacgatttgt ataagtagtt tttctcagca
      361 agccttttca atctagcaat tgcagcctcc tccgacagtg gcgactctag ctccaaactg
      421 gacttttcat agatgtcatc tggaacaatc gattgcatac actcttccat gtctttaaaa
      481 cccagaaaat taagcattgt ttttttatca tcctcactta aaccaatgtg cctggattga
      541 aacattcccc caattggcag tgaattggtg gggctgttgt tttgtttgga attggattta
      601 tttatagaat catccatctt ttgcaattgt catgattctg attgacaaag atcgttgtag
      661 tcatcaggtg aaagcaattg gttcagttca tctaaattac gatactctat tttgtaaagc
      721 cacccttgtt catcgggtga ggaattaatc acttctggct ggtcggataa tgcttcattg
      781 atttccaaga ttaccccaga aattggggaa taaatatctg aagctgcctt taaagactcc
      841 accacagcaa tttcatcatt caattgaacc tgttggccga cctctggcaa ttctacaaaa
      901 acaatatccc caagaagatc ttgggcaaaa tctgtgatgc ccacaacagc aacattatct
      961 tgctccaacc gcacccactc gtgattggtt gagaatttca actctccagt catagtattc
     1021 attgtatata atttgttaag ggtcttgttt atagacaaac tacacgtcta gaatacaaca
     1081 cgaccatttc taataaaagg cagagcaaca aattcaacct cgatgtgttt atctctgatc
     1141 gaaaccaatc cgttgtgtgc ataacctcga tccattctgg cgtaaccaat gcttgcgttg
     1201 atcaaaggag aaaaagtacc acataaaacc tctccactac cgtttggata ttttattttc
     1261 tgcccggttc gcaacatccc tttgcctttt acaatcaccc ccagtaattg tcgacatgat
     1321 ttagcatcaa tattcagaag tgcatccttg cctataaaat ttctctcagg gtccctaaag
     1381 tcgattgacc aacccaaatt ggattcaaaa ggatggtttg tttcatccat atcgacccca
     1441 tacaaattaa atcctgcctc tattcttaat gaatcgcgtg cacccaagcc gatgggttga
     1501 atattcatag caatcaaacg ttgccaagtc tcggcaactg catgatctgg aagaatcaat
     1561 tccaagccat cctcaccggt ataaccagat cttgaaacca tgattgattg atcagatgca
     1621 taccccatgt attcgaaagt gatgggttct aaaatgccta gttgattgaa actctcaaca
     1681 gcttgtggac cttgaagagc aaggattgac agatcttctc ttctcttgat acctacgtca
     1741 aatgacttta tgttttgttt gaaccaagcc ttattttttg ccatggttgt tgagttggat
     1801 acgattcggt aacggtcatc caatttatag gcaattaaat catcgatgat gcctcctgat
     1861 tcattgacta caagactata aagagatttc ttctgtgcat taattttttg gatgtcgtta
     1921 gccaataggc gcctaaaata agcgtcttga tccttaccta tgaaatcaaa cacagacata
     1981 tgcgaaacat caaaaagacc aactgagtct ctgactgcat tgtgttcagt aatctgggaa
     2041 ccataatgca agggcataga ccaaccactg aaatcaatca tttttgcgcc taaagcttga
     2101 tgggtttcat tcaagactgt ctttttcatg cgatcactca ttagttggct gagaactata
     2161 agttattatg ttcaactggg caacaagttc actgggcttg aatcaatatt tatcaatact
     2221 ctgacggtca gaaataaagt ctcgtctcgc catccccatg gatgtttccg atgcaacgtg
     2281 ggcaaagttc tgggtgatcc ttcgactggc tcaccaatgg atctctgtgc cagcatcgaa
     2341 tgcatttttg gtgttcagat tgaactactc tggcagcaat gccgtctcct tggatcgcat
     2401 ctgcgggcaa attatccata gattccactg tagcctctga agtgataaac aaaaaatgca
     2461 gctcttcacc aaacgacgac aataatgcag ccatttcacc ttcagcataa aaaacaatgt
     2521 gtgcatctag agaagaacca atcacacttt ggtctctggc aacttccaat gcctttgatg
     2581 cgattccctg tgcaagatca atgccttgcc aatcgattt
//
LOCUS       DASCTH010000175         4298 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000280249, whole genome shotgun sequence.
ACCESSION   DASCTH010000175 DASCTH010000000
VERSION     DASCTH010000175.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000280249"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="QGH35_03685"
     CDS             <1..218
                     /locus_tag="QGH35_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81668.1"
                     /translation="ITHKVKTNDDQIIDEERFTQSLQEEISKLIEERGDLSYDPEQLK
                     VATDLFHKMSTNQQFDQFLTLPAYKYL"
     gene            230..391
                     /locus_tag="QGH35_03690"
     CDS             230..391
                     /locus_tag="QGH35_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81669.1"
                     /translation="MNKQKENSTEQKKQNQESPLSRPLTEMEVIDLTIYGEDVLTPNQ
                     DQKNITTNK"
     gene            402..1694
                     /gene="aceA"
                     /locus_tag="QGH35_03695"
     CDS             402..1694
                     /gene="aceA"
                     /locus_tag="QGH35_03695"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HJL81670.1"
                     /translation="MSKQNEIQALEDKWSDERWSGITRTFSAEEVINLRGSFHIEYTL
                     ARRGAKRFWSDLHTESVISALGAMTGNQAVESVQAGLKAIYCSGWQVAGDGNSAGEMY
                     PDQSLYPVDSVPKMIERINNSLIRTDQIYHLNNDQSIDWMVPIIADAEAGFGGNLNAF
                     ELTKQMIKAGTAAIHFEDQLSSAKKCGHMGGKVLVSTQEAINKLVAARLAADVLDVPT
                     VLIARTDANAATLLQSDADERDHKFLTGNRTKDGFFSVQPGIEQAIDRGLSYAPYADL
                     LWFETAKPNLIEAERFSKEIHDRFPGKLLAYNCSPSFNWQASLNEAELGEFRERIAEM
                     GFKFQFITLAGWHALNLGMFELSKEYLKDGMLAYSNFQQREFANEPNGFRATKHQTFV
                     GTGYFDAVQTTITNGVSSTTAMEGSTEEQQFQTAVSGK"
     gene            complement(1848..3251)
                     /locus_tag="QGH35_03700"
     CDS             complement(1848..3251)
                     /locus_tag="QGH35_03700"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HJL81671.1"
                     /translation="MSSSENFLDSVNKIFDKSAALLNLTNGLAEKIKVANSTYTVRFG
                     VHLRDKLETFTGFRSVHSEHWEPVKGGIRYSPDASQTEVEALAALMSYKCSLMDIPFG
                     GSKGALIVDPKQWSKKELQRITRRYTQELAKRDLIHPSQNVPGPDLGTGEDEMAWISD
                     EYKRLHPTEIDALACVTGKPVRRGGIEGRIEATGRGIHYALKEFFEHPEDYKKAKLRS
                     DLEQQKIIIQGFGNVGYHVAKFLSEDEGALITAIIEQEGSVINDKGINIKSLKEHFSE
                     KRSFEGFDGFSTDGNKILEKKCDILIPAATESVIHSSNADQINAKLIVEAANGPVTAD
                     ADQMLAKKGVLIIPDFFANAGGVTVSYFEWVKNLTHMRFGLMQRQEQERRNSALISGF
                     EEMTGKKFPLQSKNIVVNGATEIDLVRSGLEGIMREGYKKISRKLHSDKRINDLRTAA
                     MVIAIKRIADSYYFLGI"
     gene            3384..3456
                     /locus_tag="QGH35_03705"
     tRNA            3384..3456
                     /locus_tag="QGH35_03705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3417..3419,aa:Thr,seq:cgt)
     gene            3729..4139
                     /locus_tag="QGH35_03710"
     CDS             3729..4139
                     /locus_tag="QGH35_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HJL81672.1"
                     /translation="MKVENKLRPNAKQMAGFLLGDTKTTIHMVNLLKFKDKAEYKDGR
                     QTDLTGEEAYQVYAKEVKQHLRKVGGKLIFSGEVSRLVLGEVEELWDWVAIAEYPSKK
                     AMRSMIMNKEYQKSEEHRSAGLAGQLNIETKPHK"
BASE COUNT         1363 a          822 c          866 g         1247 t
ORIGIN      
        1 ggattaccca taaagtgaaa acgaatgatg atcagataat agatgaagaa cgatttaccc
       61 agtcacttca ggaagagata tccaagctca ttgaggagcg aggtgatctc tcttacgatc
      121 cagagcaatt aaaagtggcc actgatctgt ttcacaaaat gtcgacaaac cagcaatttg
      181 atcaattttt aaccttaccc gcatacaaat atctttaaag acaatgatta tgaataagca
      241 gaaagaaaac tctactgagc agaaaaaaca gaatcaagaa agccctctct ctagaccctt
      301 aacagagatg gaagtgattg atttaacgat ttacggcgaa gatgtcttga ccccgaatca
      361 agaccaaaaa aatatcacaa ctaataaata aaggaaaaga aatgtctaag caaaatgaaa
      421 tacaggctct tgaagataaa tggtctgatg aaaggtggtc aggcataacc agaacatttt
      481 cagcagaaga ggtcataaac ctcagaggtt cttttcatat tgagtatact ttagcgagaa
      541 gaggggccaa gagattttgg agtgatctcc atactgagtc agtgatctca gctcttggag
      601 caatgactgg aaaccaagct gtggaatctg tccaggctgg attgaaagca atttactgtt
      661 cagggtggca ggtggcaggt gatggtaatt ctgcaggtga aatgtatcca gatcaaagtc
      721 tttatcctgt cgattcagtg cctaaaatga ttgaaaggat taataattca ttaatcagaa
      781 cagaccagat atatcatctt aacaatgatc aatcaatcga ttggatggtt ccaattattg
      841 ccgatgcaga agctggtttc ggaggcaatc ttaatgcatt tgaactcacc aaacagatga
      901 ttaaggcagg gactgcagca attcattttg aagatcaact ctcgtctgca aaaaagtgcg
      961 gccatatggg tggcaaggtt ctggtctcca ctcaagaggc aatcaacaaa ctagtagcag
     1021 ctcggctagc tgcagacgtc ctggacgttc caaccgtttt gattgcaaga accgatgcca
     1081 atgcagcgac tcttttacaa tcagatgccg atgagagaga ccataaattt ttaaccggca
     1141 atagaactaa ggatggtttt ttctcagttc agccagggat agaacaggct atagaccgag
     1201 gattgtccta cgcgccatac gctgatttat tgtggtttga aactgcgaag ccaaacttaa
     1261 ttgaagctga acgattctcc aaagagatcc acgatcgttt tcctggaaaa cttttagctt
     1321 acaactgttc gccttctttt aattggcagg caagtttgaa tgaagcagaa ctcggtgaat
     1381 ttcgtgaaag aattgctgaa atgggattca aatttcaatt catcacatta gcgggttggc
     1441 atgctttaaa cctgggcatg tttgagctct caaaagagta cttaaaagat ggaatgttgg
     1501 cttactcaaa ttttcaacaa cgagaatttg ccaatgagcc aaatgggttc agggcaacca
     1561 aacaccaaac gtttgttggc accggctatt ttgatgccgt ccagaccacg attactaacg
     1621 gtgtttcttc tacaacggca atggagggca gcacagaaga gcaacaattt caaacagcag
     1681 tgtctggaaa ataatactca ctaagattca ctataaaaaa aagggctctg tgcagagccc
     1741 ttttaattgt taattggtta caaactttga ttagttgtaa agaccaacca aaattaagca
     1801 gtgtagtcaa aaaatgaaac tttggtgatt gtagtagagg gctttcttta aatacctaaa
     1861 aaatagtaag aatcagcaat tcttttgatt gctatcacca tggccgcagt tctgagatca
     1921 ttaatgcgtt tgtctgaatg caatttcctg ctaatttttt tgtatccctc acgcataatg
     1981 ccctcaagtc ctgaacgaac caggtcaatc tcggttgcac cattgacgac aatatttttt
     2041 gattggagag ggaacttttt accagtcatt tcttcgaatc cactaattaa tgcagagttt
     2101 ctcctttctt gctcctgtct ttgcatcaac ccaaaccgca tgtgggtcaa attttttacc
     2161 cactcaaaat aagaaacagt cacccctcca gcattggcaa agaaatcagg gataatcaag
     2221 acacctttct tggcaagcat ttgatcagca tcagctgtga ctggtccatt ggctgcttca
     2281 acgatcaatt ttgcattgat ttgatctgca ttacttgagt gaatgacact ttctgttgct
     2341 gccggaatta agatatcgca ttttttttca aggatcttat ttccatcagt gctaaatcca
     2401 tcaaaacctt caaaacttct tttttcacta aaatgttctt taagtgattt aatattaata
     2461 cccttgtcat tgatgacaga accctcctgt tcaatgatag ccgtaatcag agctccttcg
     2521 tcttctgaaa gaaatttagc gacatgatag ccaacattac caaagccctg aatgataatt
     2581 ttttgttgct ctaggtctga gcgtaacttg gcttttttat aatcttctgg gtgttcaaaa
     2641 aactccttga gagcataatg gattcctctc cccgtagctt caatcctccc ttcaatgccg
     2701 cctcttcgaa cgggttttcc tgttacacaa gccagggcat ctatttcagt tggatgcaat
     2761 cgtttgtatt catctgaaat ccatgccatt tcatcttcac ctgtacctaa atcaggtcca
     2821 ggaacatttt gcgaaggatg aattagatct ctcttggcta actcttgtgt ataacgtcga
     2881 gtgattcttt gtaattcttt tttgctccat tgttttggat ctacaatcaa ggcacctttt
     2941 gagccaccaa agggtatatc cattagtgag catttataag acattaatgc tgcaagtgcc
     3001 tcaacttctg tttgtgaggc atcaggtgaa taccgaatcc cacccttcac tggttcccag
     3061 tgttctgagt gaaccgacct aaagccagta aaggtttcaa gtttatctct tagatggaca
     3121 ccaaatcgga ctgtgtatgt tgagttggca acttttattt tctcagccag gccattggtt
     3181 aggttcaaca aagcagcaga cttgtcaaaa atcttgttga ctgagtctag gaaattttca
     3241 gaactagaca tgatgctgtt attctttctt tttctctatt tgcaatagat aattaatgga
     3301 catgatacat ctaattttga ttggcacaat acaaataatg ggaaaagatt ggtgcacaaa
     3361 tggttctgat aatttatgaa attgccggaa tagctcagtt ggtagagcaa ctgattcgta
     3421 atcagtaggt cgtaggttcg actcctgctt ccggcaccgc tttaaaggct tattgctatt
     3481 gatatataga gtttataaac tttgtgcagt caatagcagt aagtgaaatc ttagacataa
     3541 aataccactt ttgacactct ccttgagtta gatcattgca agttttgcag gtttaggagt
     3601 atgttattgt ttaggtatct aaggatttaa ttaaaggaga tagcagagat gactaaaact
     3661 tttcgagttt gtaataagcc agtaggaatt atgaccaatg aaacagtaaa tagaatcaag
     3721 agcatggcat gaaagttgaa aataaactca gaccaaatgc taaacagatg gctggatttc
     3781 ttcttggtga tacaaaaaca accatccaca tggttaattt attaaagttt aaagacaaag
     3841 ccgagtataa agatggaagg caaactgact taactggtga agaggcttac caagtatacg
     3901 caaaagaggt taaacaacat cttcgaaaag tgggtggaaa acttatattt agtggggaag
     3961 tgagtcgatt agttcttgga gaagtcgaag agctctggga ttgggttgca attgcagaat
     4021 acccaagcaa aaaagccatg cgttccatga taatgaataa ggaatatcaa aaaagtgaag
     4081 agcatcgaag cgcaggctta gcaggtcaat taaatataga aaccaagcct cacaaatgag
     4141 gttggtcttt tagtagcata ataaatgaaa aaactcatcc tcatatcagc actactgctc
     4201 tttggttcta atggttggac tgatgactta ccaaccgatg gtgaatttca aatcaaccac
     4261 cccaatggaa agctttatct caaaggagag cttaagaa
//
LOCUS       DASCTH010000176        11173 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000280522, whole genome shotgun sequence.
ACCESSION   DASCTH010000176 DASCTH010000000
VERSION     DASCTH010000176.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000280522"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="QGH35_03715"
     CDS             <1..297
                     /locus_tag="QGH35_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HJL81673.1"
                     /translation="QGVHLPVWVGLPGAIERGRLLRTSLRIGVGNSLRFLRKKSQVAA
                     ELMKSSIYNPDKLVTSISEYKDIANTNLAGYHIFCFNQIEITEKWRTETISKLM"
     gene            484..1548
                     /locus_tag="QGH35_03720"
     CDS             484..1548
                     /locus_tag="QGH35_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017310362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HJL81674.1"
                     /translation="MKEKYIEEYLAVALQLDCHAINNCQTKEESHDLMMRRIQDISNF
                     VQTTVNFHLFFYGSPVKLVVLPEYTVCGFPLQETPQEWREKGCFEIDGPEYEAFSKIA
                     QDNDIYLAGNTYEIDPNFPEIYFQTCFIIDPSGDVIVRYRRLTSVIWATPHDVWDKYL
                     DLYGYEKVFPVAKTEIGNLACIASEEIMYPEIARCHVMRGAEILLHPSSEPGSPRSTE
                     KEICRRARAAENLAYVITANTSTILNISVPAYTCSGMSKIVDYRGKVLSEASAGGESQ
                     TANAFINLRSLRKYRRNAGMTNYVSRQGFDAYAQSYAEADFLKANSLLKDGKVQQPSR
                     EFFLQRQEKVIEKLIAKGIL"
     gene            1585..4308
                     /locus_tag="QGH35_03725"
     CDS             1585..4308
                     /locus_tag="QGH35_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012602.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HJL81675.1"
                     /translation="MRLSRRKFLSGTGVAASALSLNYAHPVQGGDDSYRVWEDLMRTK
                     WTWDKVAHSSHGVNCAGHCAFKVFVKNGIVWREEQQGEYGATEDAPDYNPRGCQKGVR
                     HSKYMYGKQRVLYPLKRVGERGEGQWQRISWDQACDEIADKYIDHWLSSGPASMSFAM
                     GTAMLLKKATIAGYQRFLNLTGIPAPETFAGVGDQPIGIYQTLGLDITGDTVAAVYKA
                     KCVLVWFCNPAVTRIPDAHFFWEARYNGTKVIVISPDFNPSAMHASKWVNPKPGSDGA
                     LAMAMAQVILKDKSYDTDYIKEQTDLPMLVRTDNGKFLHPKDLGKAGGNEIYYLWDEA
                     SNGIQQAPGKDRETIELGALQPSLEGTWNIDTNSGPLAVTTVFELLKKRAEEHTPEVT
                     AAITGISPEVIREIARDFASAKPAMIYGGFRSAKWLYGDIFQRAMLLLLSLTGNLGNE
                     GGGYQLTQLSKSDGIAPYLFSDIGYGMRIKAAAMWDYEHMDLKEVNRKFYGDELADTY
                     DEHYQESLDKGWWPRQKEKPLKMAIFGGHNPGSWKAGGDHWAENIYEKVETIVSIIPD
                     MGITHLYADYVLPVAHHYERHDLLVQPKTPYLQAMDKVVEPLGESVDDFTVFKRLSKV
                     ISKKAKERGIQPIMDTFVDGRPPIERDLTKLYDQLTWGGKFNDSKDIAEFLINASPGL
                     PRITFDELAAKGIVRVEDSEGVVYGDNAPYATMKQYVEHKKHYETHTGRQQHYHDHDW
                     YIKYDETLPGHKDPLELEGYPLRMLMGHARHSIHSSWRDDTFLWSLQRGEPDIYVNPN
                     DAKDRGVADGDLIKIFNTMGSFIAVAHISSSMQQGMMFMYHGWDPTMFRNQKNFSSVI
                     TTAGLIRPTLLAGGADTHLGYAPIKSFPNMTLSDCTCDFELHAESNEQKTA"
     gene            4344..5057
                     /locus_tag="QGH35_03730"
     CDS             4344..5057
                     /locus_tag="QGH35_03730"
                     /inference="COORDINATES: protein motif:HMM:NF022750.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HJL81676.1"
                     /translation="MNTKLTSIFLGLCLSLIVSSACWSQPQNFVAVYTAQLKQADGQL
                     SMTLKKEDGGLYSYELTTKPGGFWRIIIDGSIWQKSTFILEGNDVLRSQTYELIDTIR
                     SKPRKSRASFDWEDSLITGHYKDRAIKLPLTGNIIDKVSLQIAIIMDIRRGIDQSEYY
                     ILDKDKIQVVQVNRNSSAMINVPFGKFEAIEINRSSPDSNRTNTLWLAPELDYIPVKI
                     TQEENGKIVFSASLSQLTH"
     gene            5119..5712
                     /locus_tag="QGH35_03735"
     CDS             5119..5712
                     /locus_tag="QGH35_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HJL81677.1"
                     /translation="MNGIESIMKSAEKLFSKKSFTAVSMGEIALEAGISKANIYHHFD
                     SKEDLYQKILLRCQNNSFSDVLEAEKHPGTYIDKIKFFMHTRMEDMLSNPQPYTLLLR
                     EISDLSGSRGNSYDQNLLSGAIDRVVKLIEQAQSHGEISTNVDPLIIAFQLMAPNFMY
                     ILFEQILSERIGHIDPKQTKQFCELLANNLLNGIAQS"
     gene            5709..6788
                     /locus_tag="QGH35_03740"
     CDS             5709..6788
                     /locus_tag="QGH35_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HJL81678.1"
                     /translation="MIGQSKKIKNIIVKLPILTLLMYGLTPVPLSMAAVAITTGKVAT
                     TTIEEKESAMGIIYSLSSPKVASEVSGRVIKVLIDVGDAVQQGQIMAEIDPERYELET
                     IQAKAETARLSALKINQELDLSRAERLYKNNLVSEEILDKTKAELEALEQQIKAAEAK
                     HNSSKKLLRDTKIKAPITGYVAAKKIVMGDFVQTGTIVFELVDTKNLRVGVSFPEYQS
                     SKLKKGLKTYLTSPTNTNHIVETQINEVSPNINPKNRSISVIIDFENPGLWLPGASAR
                     AEIILSERENALVVPQLAVVRRSIGNVVYVIKNSVAIETKVDTGIHLDGLIEIKAGLQ
                     KGTLIAVDGAGFLADQSQVIIESNQ"
     gene            6785..9877
                     /locus_tag="QGH35_03745"
     CDS             6785..9877
                     /locus_tag="QGH35_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HJL81679.1"
                     /translation="MSLAEISIKRHVFATMINLVVVLFGIISINRIAIDRTPDIDFSL
                     LAVTTVLPGANPDVVDSSVTNIIEGAVNSIAGIDELRARSETGFSTVFPTFVLEKDLD
                     VAFNETQEKVNQVIAQLPTDTETPIINKIATGEIPAMWLALTGDRTLQELDLYARNII
                     KRKLETIKGVAKISIGGGRERNIRVNLNFDRMSAFGITVQDIVRAFRNEHIKLPGGFL
                     IENSKEELLKLDLEYHSPEDVGNIIVAYTNNNPIKLKNIAQIEDDVSDLRNLARFQGK
                     PAIGLGVGKIRGENTIEVIEAVKQRIETDILPFLPDGMMLDYATDDSEYIYEIITGLG
                     QHLIAGAIFAALVVLLFLRSFIGMGIVAITIPVSLLGSIVVMYFFGYSLNTMTMLGLL
                     LLIGVVVDDAIVVLENSQRHLDTSGLDRIRSTIIGTNEVFLPIVASTLTLAAIFTVVI
                     FLEGIAGRFFRSFGVVVSSGVIISLFISLTLTPMLCSQWLKPSTSINKFFERVQNFFI
                     GLEERYRNLVYWAINNKRKVVISGLVIVSSTALFFQNLEGSFFPKEDEGQFSVTVKTP
                     LGSGIEYSNDRVGKVEEILRNYEDIDSLFSIVGGTSGDGASGTQINESAIYVKLKPRT
                     ERTLKQYELIPLIRAKLAEVPGVKAFPSPLSFASGTRGEALQFTLAGPDLYVLDEVAK
                     IFLAKLSETPELGNVDMSMDMEMPQVALDINRELVSELGLSTKIVAEAANVFAGGIDV
                     ARYNDSIGGGERYKIRLKAEQNMSISDLNKIYLRTPNADLVRLDTVAAFSEIVGPASI
                     SRFNREYASYFFTDPTMALSDAIDTVESAAAETLPPGYHVNFFARAKEFKKTSNTMLI
                     AFLTGLLLVYMVLASQFNSFSQPLIIMVSQPLAVLGGIFGLWIAGIALNIFSMTGLVL
                     LTGLVAKNSILLVDRANEEMKTGKNADEALLVACPRRLRPILMTSFTIILAMLMPALG
                     IGAGIELSQPLAVAVVGGMFSSTLLTLILVPVVYSWAEDKKRAPIRKSLGVLVGAIRK
                     "
     gene            complement(9834..10751)
                     /locus_tag="QGH35_03750"
     CDS             complement(9834..10751)
                     /locus_tag="QGH35_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HJL81680.1"
                     /translation="MAAVICGSVAYDNIMGFDGKFSEYILPDQLDILSVAFTVDLRKE
                     FGGCAGNIAYNLRLLGDEPLIVATVGNDFDAYEAWLNKNHIDTGGIKTIPEKLTAQCF
                     ITTDSDNNQLTSFHPGAMEDGHLNTIPDHAETTIGILGPASTKGTLEHADQLVKKSIP
                     FIFDPGQSTPMFNKDELNQLIDLSNWIIVNQYEANLLSDKVEKSIQDISNQVEAMIVT
                     KSGEGSEMFVGEEVIAVSPYKAERVCDPTGCGDAYRAALLYGIPRSMSWDEMGQMGSL
                     MGAIKVAQYGTQNHQFTYESLQQELQDSS"
     gene            complement(10797..>11173)
                     /locus_tag="QGH35_03755"
     CDS             complement(10797..>11173)
                     /locus_tag="QGH35_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013984415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HJL81681.1"
                     /translation="IKSITKGLQKLGILSGNIDQLIANEAYKPFYMHGIGHWLGMDVH
                     DVGSYGNPKKGSVRKFEPGMVLTIEPGIYISTKNRQVEKQWRGIGIRIEDDILVKKTG
                     NEILTHQLPKEIDDIESIMANH"
BASE COUNT         3460 a         2200 c         2358 g         3155 t
ORIGIN      
        1 caaggcgtcc atcttcctgt ttgggtcgga ttgcctggtg ccattgagcg aggacgtctt
       61 cttagaactt ctcttagaat aggtgtcggg aactccctgc gtttcttgcg aaaaaaatcc
      121 caagttgcgg cagagttaat gaaatcaagt atctataatc cagataaatt ggtaacaagt
      181 atttctgagt ataaagatat tgctaacacc aatcttgctg gataccatat attttgtttc
      241 aaccagattg aaataactga aaaatggcgt actgagacaa tttcaaaact aatgtaaacc
      301 cttgaaaagt gtaaaggcat aataagatcg gagaaaacaa tgaggataaa cgatacagag
      361 aatgccatgt ttgatgacaa aggcacattg catatcgatg gcgcttctta ctcataatcc
      421 attggtcgga ggtttgagtc ctcccgggtc caccaaaatt attaatatca aaggtaagta
      481 gaaatgaaag agaaatatat tgaagaatat ttggccgtag ctcttcaact ggattgccac
      541 gcaatcaata attgccaaac caaagaagag tcacatgatt taatgatgag acggattcaa
      601 gatatatcca attttgttca aacaacggtc aactttcatc tcttttttta tggctcacca
      661 gttaaattag ttgtccttcc tgaatacact gtttgtggat ttcctttgca ggaaacccct
      721 caagagtggc gagaaaaagg ctgcttcgag atcgatggtc ctgaatatga ggcattttca
      781 aaaattgctc aagacaacga tatctatctg gcaggaaata cctatgaaat tgatccaaat
      841 tttccagaaa tctattttca aacctgtttt atcattgatc catctggtga tgtaattgtt
      901 agatacagaa gactaactag tgttatctgg gcaacaccac acgatgtctg ggacaagtat
      961 cttgatttat atggatatga gaaagttttc ccagtagcaa aaacagaaat cggtaatctt
     1021 gcctgtattg cttctgaaga aatcatgtac cccgagattg cacgctgcca tgttatgaga
     1081 ggggctgaaa ttctcctaca tccaagcagt gaaccgggta gcccaagatc aaccgaaaaa
     1141 gaaatttgtc gcagagctcg agccgcagaa aatcttgctt atgtgatcac cgcaaatacc
     1201 tcaacaatac ttaatatctc cgtccccgcg tatacgtgca gcggtatgtc taaaattgtt
     1261 gattacagag gaaaagtttt atctgaagcc agtgcaggag gtgaaagtca aacagccaat
     1321 gctttcatca accttcgttc actcaggaaa taccgcagaa atgcaggaat gactaattat
     1381 gtctcaagac agggctttga tgcttatgca cagagttatg cagaagcaga tttcttaaaa
     1441 gcaaactcat tacttaaaga tggcaaagtc caacaaccaa gtagggagtt ctttcttcaa
     1501 aggcaggaga aagtgatcga gaaactgata gctaaaggta tattgtagac agcacatttg
     1561 gtttaagaga gaattaacta agttatgcgc ttatcaagaa gaaagttttt atcaggaacc
     1621 ggtgtagcgg cttcagccct cagccttaac tacgcacacc ccgttcaggg aggcgatgac
     1681 tcatatcgag tctgggaaga tttaatgaga acaaaatgga cttgggacaa agtagctcac
     1741 tcaagccatg gagtaaattg tgcagggcat tgtgcgttca aggtctttgt taaaaatggt
     1801 atcgtctgga gggaagaaca acaaggtgaa tacggggcca ctgaagatgc ccctgattac
     1861 aatcccagag gctgtcagaa aggagttcgg cactccaaat acatgtatgg caaacaacgg
     1921 gttctgtacc cacttaaaag agttggggag cgtggcgaag gacaatggca gagaatttca
     1981 tgggatcagg catgtgacga aattgcggat aaatacattg accattggct cagtagtggc
     2041 ccggcttcga tgtcttttgc catgggcaca gcaatgctat taaaaaaagc aaccattgca
     2101 ggctatcagc gttttttaaa cctcaccggt attcccgcac cagaaacctt tgctggagtt
     2161 ggtgaccaac ccattggcat ctatcagaca ttaggccttg atatcactgg tgataccgtg
     2221 gctgcggtgt ataaggccaa gtgtgtattg gtatggtttt gcaatccggc agtgacccga
     2281 attccggatg ctcatttctt ttgggaagcc agatacaatg gcaccaaagt tatcgtcatt
     2341 tccccagact ttaatcccag tgccatgcat gcttccaagt gggtcaatcc caagccaggc
     2401 tctgatggtg ccctagcaat ggcgatggca caggtcatac taaaagacaa aagttacgat
     2461 accgattaca ttaaagaaca aaccgatctg cccatgctgg tacgcacaga taacggcaaa
     2521 tttttgcatc caaaagacct aggtaaggca ggcggtaatg aaatttatta cctttgggat
     2581 gaggctagta atgggattca acaagcacct gggaaagacc gtgaaaccat tgaattggga
     2641 gcactgcagc ccagtcttga aggcacatgg aacattgata ccaacagtgg accgctagca
     2701 gtcaccacag tatttgaatt acttaaaaaa cgagcagaag aacacactcc tgaggtcacc
     2761 gctgcaatca ccggtattag cccagaagtc attcgagaaa ttgccagaga ctttgcctca
     2821 gccaaaccag ccatgatcta tggtggcttt cgatcggcga aatggctgta tggggatatc
     2881 ttccaaaggg caatgctgtt attactgtca ttgaccggta atctgggcaa tgaaggtggt
     2941 ggttatcagc tgacccaact ttcaaaatca gatggtatag ccccttacct tttctccgac
     3001 attggttatg gcatgagaat taaagcggcg gcgatgtggg attatgagca catggattta
     3061 aaagaagtga accgtaaatt ttatggtgat gaattagctg atacctacga tgaacattac
     3121 caagaatccc ttgacaaagg atggtggcca agacaaaaag aaaagccctt gaaaatggcc
     3181 atctttggag gacacaaccc aggcagttgg aaagccggag gagatcactg ggcagaaaat
     3241 atttatgaga aagttgaaac tatagtatcc attatacccg acatgggtat cacccacctg
     3301 tatgccgatt acgtcttgcc ggttgcccat cattatgagc gacacgattt gttagttcaa
     3361 ccaaaaacgc cctacttgca agccatggat aaagtggtgg aaccattagg ggagtcggtg
     3421 gatgatttca ctgtttttaa acgtttatcc aaagtcatca gtaaaaaagc caaagaaaga
     3481 ggcattcaac cgatcatgga tacatttgtc gatggaagac ctcccatcga gagagacctc
     3541 actaaactct atgatcaact tacatgggga ggcaaattca acgactctaa agatattgca
     3601 gaatttttga ttaatgcctc accgggactg cctaggataa cctttgatga actggctgca
     3661 aaaggcattg tcagagttga agactccgaa ggggtggttt atggggacaa tgctccttat
     3721 gcaaccatga aacaatatgt ggagcataag aagcactacg aaacccatac cggtagacaa
     3781 cagcattatc acgatcacga ctggtacatt aaatacgatg aaaccctgcc cggtcacaaa
     3841 gacccccttg aactggaagg ctatccactg agaatgctta tgggacatgc cagacacagt
     3901 atccacagtt cgtggagaga cgatactttt ttatggagcc tacaacgagg tgagcctgat
     3961 atatatgtca atcccaatga tgccaaagac cgaggcgttg ccgatggtga tttaatcaag
     4021 atcttcaata ccatgggttc tttcattgcc gttgctcata tttcctcaag tatgcaacaa
     4081 ggcatgatgt ttatgtatca cggctgggat ccgaccatgt ttagaaatca gaagaacttt
     4141 agttcggtca taaccactgc cggtcttatt aggccaactc tattggcagg tggtgcagat
     4201 acccatttgg gttatgcgcc cattaaatcc tttccaaata tgacactcag cgattgcact
     4261 tgtgattttg agctgcatgc tgaatcaaac gagcagaaaa cagcctagac aaatttgctc
     4321 tgggttgtag atttgaattg gtcatgaaca ctaagctgac aagcattttc ttaggcttgt
     4381 gcttgagcct cattgtaagt tctgcctgct ggtctcagcc tcagaacttt gtagctgtct
     4441 acactgcaca actaaaacaa gcagatggac aattatcaat gactctaaaa aaagaagatg
     4501 gaggattgta cagttatgag ctaacaacaa agccaggtgg attttggcga attattatcg
     4561 atgggtcaat atggcagaaa agcactttta tactagaagg caatgatgtt cttagatctc
     4621 aaacatacga attaatagat accattcgca gcaaacccag aaaatccaga gcaagttttg
     4681 actgggaaga ttcactgatc acaggccact ataaggacag agctataaaa ctacctttga
     4741 ccggaaatat tatagataaa gtctcactgc aaatagccat tattatggat attagaagag
     4801 gtatagatca atctgaatac tatattttag ataaagataa aattcaggtt gtgcaagtaa
     4861 atagaaatag ttcagcaatg atcaatgtgc cttttggtaa atttgaagcc attgaaatca
     4921 atcgttccag tccggactca aatagaacta atactttatg gttagctcca gaattagact
     4981 atataccggt aaagataacc caggaagaaa atgggaaaat tgttttttca gcaagtctga
     5041 gtcaattgac tcattaagga ttgaaaagct tttttttaag atatatactg accgaccgat
     5101 cggtaagtat atagcgtgat gaatggcata gaatcgataa tgaaatcagc tgaaaagctt
     5161 ttctccaaga aaagttttac ggccgtgtcc atgggtgaaa ttgcacttga agcgggtatc
     5221 agcaaagcca atatttacca tcacttcgac tcaaaagagg acctgtatca aaaaatactg
     5281 cttcgttgcc aaaataattc cttctctgac gtactggaag ctgagaaaca cccaggtact
     5341 tacatcgaca agattaaatt cttcatgcat acaagaatgg aagatatgct aagcaatcca
     5401 caaccctata cccttctgtt acgggagatc tccgacttaa gcggttcaag aggaaacagt
     5461 tacgaccaga acctattgtc tggagccatt gatcgagtgg taaagctgat tgaacaggcg
     5521 caaagtcatg gagaaatttc cacaaatgtt gaccctttaa tcattgcttt tcaattgatg
     5581 gcaccaaatt ttatgtacat cttatttgag caaattctca gtgaaaggat cggtcatatt
     5641 gatcccaaac agactaaaca gttttgtgaa ttgctggcca ataacctgct taatggaatt
     5701 gcacagtcat gattggtcaa tctaaaaaaa taaaaaatat tattgttaaa ttaccgatat
     5761 tgacgctttt aatgtatggg ttaacacctg tgcctttgag tatggctgct gtcgccatca
     5821 ctacaggcaa ggtagcaaca acaactatcg aagaaaaaga aagcgcaatg ggcatcatct
     5881 atagtctatc ctctcccaag gttgcttctg aagtctctgg aagagtcatt aaagtgttga
     5941 tcgatgttgg cgatgctgtt cagcaaggac agatcatggc agaaattgat cctgaaagat
     6001 atgaattgga aaccattcaa gccaaagcag aaactgctcg attatctgca ctgaagatta
     6061 atcaggaatt ggacttatct cgggctgaaa gattatataa aaacaatcta gtttccgaag
     6121 aaatactcga caaaacaaaa gccgaactgg aagcattgga acaacaaatt aaagccgctg
     6181 aagcaaaaca taatagcagc aaaaaattgc tcagagacac caagataaaa gcacctataa
     6241 ccggttacgt agcagcaaaa aaaatagtga tgggtgattt tgtgcaaaca ggcacgattg
     6301 tttttgaatt ggtcgataca aagaatttac gggtgggtgt ttcctttcca gaataccaat
     6361 cctcaaaatt gaaaaaagga ttaaaaacct acctgacctc ccccaccaac accaatcaca
     6421 ttgtagaaac tcaaatcaat gaggtcagtc ctaacatcaa cccaaaaaat cgctcaatca
     6481 gtgtcattat tgattttgaa aatccaggct tgtggttgcc tggtgctagt gctcgagctg
     6541 aaataatctt atcagaaagg gaaaacgctt tagtggtacc acagcttgcc gtggttagaa
     6601 gatctattgg caacgtggtt tatgtgatta aaaactcagt agcaatagaa acaaaggttg
     6661 atacaggaat tcatctcgat ggattgattg aaataaaagc tggtcttcaa aaaggtacat
     6721 tgatcgcggt tgatggcgca ggcttcctag ctgatcagag tcaagtgatt attgagagca
     6781 accaatgagt ttagccgaga tttcaataaa gcgacatgtc ttcgctacaa tgatcaactt
     6841 agtagtggtg ttgtttggca ttatttcaat taacagaata gcgattgata gaacaccaga
     6901 tattgatttt tcactgcttg ccgttaccac cgtattgcct ggagcaaatc ctgacgtggt
     6961 ggactctagt gttaccaaca tcatagaagg agccgtcaat agtatcgcag gtattgatga
     7021 actcagagcc agatcagaaa caggattttc tactgttttt cctacttttg ttttggaaaa
     7081 agatctcgac gttgctttta atgaaaccca agaaaaagtt aatcaggtta ttgctcaatt
     7141 acccaccgat accgaaaccc ccattattaa taaaatcgca acaggagaga tacctgcaat
     7201 gtggcttgca ctaacgggtg atagaacact ccaagaattg gatttatatg cgaggaacat
     7261 tattaagagg aagttggaaa caataaaagg agttgcaaaa atttcaattg gtggtggacg
     7321 tgaaagaaat atcagggtca atcttaattt tgatcgtatg agtgcttttg gcatcaccgt
     7381 ccaggacatt gttagggcat tcagaaatga gcatattaaa ttacccggtg gtttcttaat
     7441 agagaattca aaagaagaat tattaaaatt agatcttgaa taccatagcc ctgaagatgt
     7501 aggcaacatc attgttgcct acaccaacaa taatccaatt aaactcaaga atattgccca
     7561 gattgaagat gatgtttcag atcttagaaa tttggcacga ttccagggaa aacctgccat
     7621 tggccttgga gtgggtaaga tcagaggaga aaataccatt gaggtcattg aagctgtaaa
     7681 acagagaatt gaaacagata ttctcccttt tttaccagat gggatgatgc ttgattacgc
     7741 aactgatgac agtgagtaca tctatgaaat cattacaggt cttggacagc atttgattgc
     7801 aggggcaatt tttgcagcat tggtggtgtt gttattctta agaagtttta ttggcatggg
     7861 tattgttgcc atcaccatac ccgtatcttt actgggttct attgttgtaa tgtatttttt
     7921 tggatacagc ttaaatacta tgacgatgtt gggattgctg ctactcattg gagtggttgt
     7981 cgatgatgct attgttgttt tagaaaattc ccagcgacat cttgatacca gtggattgga
     8041 tagaattaga tcaaccatta ttggtaccaa tgaggtattt ttgccgatcg ttgcttccac
     8101 tctcactctg gctgctatat ttactgtggt gatcttttta gaaggcatag cgggtcgttt
     8161 cttcaggtca ttcggagtag tcgtctcttc tggggtgatc atatctttgt ttatttcgct
     8221 gacattaact cccatgctct gctctcaatg gttaaaaccc tcaacgtcaa taaataaatt
     8281 ctttgagaga gttcagaatt ttttcatagg tcttgaagaa cgctatagaa acttagttta
     8341 ttgggctatt aacaataaac gaaaagtagt gatttctgga ctggtcattg tgtcttcaac
     8401 agctttattt tttcaaaatc ttgaaggttc cttctttccc aaagaagatg aaggacaatt
     8461 cagtgtcact gttaaaacac ccttgggatc tggtattgaa tacagtaatg acagagttgg
     8521 aaaagtcgaa gaaatcttaa ggaactacga agacatcgat agtttgttta gtattgttgg
     8581 tggaacttct ggcgatggag cttctggcac gcaaatcaac gaaagtgcca tttacgttaa
     8641 gttaaaaccg aggacagaaa gaacattaaa acaatacgag ctcattcctt taattagagc
     8701 aaaattagcg gaagtgccag gggttaaagc attcccctcc cccctttcat ttgcttctgg
     8761 cacaagaggt gaagccttac aatttactct cgcgggacct gatttatatg ttcttgatga
     8821 agtagcaaaa atatttcttg caaaattaag tgaaacacct gaactcggaa atgtcgatat
     8881 gtccatggac atggagatgc cacaagttgc tctggatata aaccgagaac tggtttcaga
     8941 acttggatta tcaacgaaaa tagttgcaga agcagccaat gtctttgctg gtggtattga
     9001 tgttgccaga tacaatgact caattggcgg aggtgagcgt tacaagattc gtctcaaggc
     9061 agaacagaac atgagtatca gtgacttaaa taagatttat cttcgaactc caaatgctga
     9121 tttagtgaga ttggatacgg ttgcagcttt ctcagagatc gtaggaccgg caagcatatc
     9181 gagatttaac agagaatatg cttcttattt ttttacggat cctactatgg ctcttagtga
     9241 tgccatagat actgtcgagt ctgctgctgc agaaacattg cctcctggtt atcacgttaa
     9301 cttttttgca agggcaaaag aattcaaaaa aaccagcaat acgatgctta ttgcttttct
     9361 gacaggattg ttactggttt atatggtttt ggccagtcaa ttcaattcat tcagccaacc
     9421 actgatcatc atggtctccc aacctctcgc tgtgcttggt ggtatctttg gtctttggat
     9481 tgcaggtata gctttaaata ttttttccat gactggtctg gtattactga ccggcttggt
     9541 tgcaaaaaat tctattttgt tggttgatcg tgccaatgag gaaatgaaaa ctggaaaaaa
     9601 tgcagacgaa gcactgcttg tagcttgccc aagaagacta aggcctatac tgatgacttc
     9661 cttcacgatt atcttagcta tgctcatgcc tgccctaggt attggtgcag gaattgaact
     9721 cagccaacct ctggccgttg cagtagttgg tgggatgttc agttctaccc tattaactct
     9781 aatcttagtg cccgttgttt actcttgggc agaggataaa aagcgtgctc caattaggaa
     9841 gagtcttgga gttcttgttg gagcgattcg taagtgaact gatgattttg tgttccgtat
     9901 tgagccactt ttatagcacc cattaaagag cccatttggc ccatttcatc ccagctcatg
     9961 cttctgggta tgccatataa taaagcagct ctataagcat caccacaccc tgtagggtca
    10021 catacccttt cagccttata aggtgataca gcaattactt cttcgccgac aaacatttca
    10081 gaaccttcac cactcttggt aacaatcatg gcttccactt gattgctgat gtcttgaatc
    10141 gatttttcca ctttatcaga cagtaaattg gcttcgtatt gattgacaat aatccagtta
    10201 gataaatcaa ttaactgatt cagctcatct ttgttgaaca tcggagtact ttgacccgga
    10261 tcgaaaataa acggtataga ttttttcacc agttgatctg cgtgctccag tgttcccttg
    10321 gttgatgctg gacctaagat acctattgtt gtttctgcat ggtctggaat tgtatttaaa
    10381 tgcccatcct ccatcgctcc tggatgaaat gaagtgagtt gattgttgtc tgagtcagtg
    10441 gttataaaac actgtgcagt caatttttct ggaatggttt taatgcctcc cgtgtcaatg
    10501 tgatttttat tcaaccaagc ttcataggca tcaaaatcat tacctacagt agcaactatc
    10561 aaaggttcat cacctaataa tctaaggtta taggctatat ttcccgcaca gccgccaaat
    10621 tccttcctga gatctaccgt aaatgcaaca cttaagatat ccagctgatc aggcagaatg
    10681 tactcgctaa atttcccatc aaaacccatg atgttgtcgt aagcaacgga gccacaaatt
    10741 actgctgcca taactatatt ttcctcatgt tgaagttgtg ataattcatt gttgaattaa
    10801 tgattagcca taatagactc gatatcatca atttcctttg gaagttgatg agttaaaatc
    10861 tcattgccag tttttttaac cagaatatca tcctctatcc tgataccgat acctcgccat
    10921 tgcttctcca cttgcctatt tttagtagag atataaattc ctggttcaat ggtgaggacc
    10981 atccctggtt caaactttct cactgatccc tttttgggat tgccataaga accaacatca
    11041 tgcacgtcca tacccagcca atgacctata ccatgcatat aaaaaggttt atatgcctca
    11101 ttggcgatca attgatcgat attgccactc aagataccca atttctgtaa cccctttgtg
    11161 attgatttga ttg
//
LOCUS       DASCTH010000177         3093 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000283077, whole genome shotgun sequence.
ACCESSION   DASCTH010000177 DASCTH010000000
VERSION     DASCTH010000177.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000283077"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /gene="pmbA"
                     /locus_tag="QGH35_03760"
                     /gene_synonym="tldE"
     CDS             complement(<1..590)
                     /gene="pmbA"
                     /locus_tag="QGH35_03760"
                     /gene_synonym="tldE"
                     /inference="COORDINATES: protein motif:HMM:NF040244.2"
                     /note="protease involved in proteolytic processing of the
                     antibiotic Microcin B17 and in sensitivity to the DNA
                     gyrase inhibitor LetD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HJL81682.1"
                     /translation="MNNKILETSIEIALEHANKLGADQTEASCSNGKGFSVQAQNESI
                     ETIEHYSSSAFSVTVYANQSIGSATTNDLSQNALITTTEKAFSLANLTARDQFNGLAD
                     AHLMAQKPMDLKLYHPWELNVEQAQTMSLECEQAALDTDSKVENTEGANVATYENRTV
                     YANSHGFVGLTESTGHTISCTAIAAENGSMEMDYDYT"
     gene            664..1146
                     /gene="purE"
                     /locus_tag="QGH35_03765"
     CDS             664..1146
                     /gene="purE"
                     /locus_tag="QGH35_03765"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HJL81683.1"
                     /translation="MNIDVAIIMGSKSDWDIMSHSANILTQFDIAHESKVISAHRTPV
                     LLDEYCSEAQQKGVKIFITGAGLAAALPGVVAAKTTLPVIGVPLEASSLDGMDALLSI
                     VQMPPGIPVGTMTIGKAGAINAALYAAAILGLQDENIREKIQSYRDEQAQKIIDTNLE
                     "
     gene            complement(1151..2596)
                     /gene="tldD"
                     /locus_tag="QGH35_03770"
     CDS             complement(1151..2596)
                     /gene="tldD"
                     /locus_tag="QGH35_03770"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HJL81684.1"
                     /translation="MNNSESTLAQVKSILLEPYNLTEQDLALMLSTLLSNGSDMGDIY
                     FQSMCYESWTLEDSQVKKSVFSNKQGVGFRSVVGEKSGLAYSETFDKESLLNAVKAAK
                     SIANKQKNYSVKIEPRTTMQHLYPSTDPVGSLSDNNKIKILELIDSHARAKDHRVKEV
                     VATLAGSYQTILIINQQGCLTTDIRPMVRLSVNVIVEENGRRESGSSGGGGRIDYSLF
                     NNKAIYTYAEEAVEQALVNLEAKEAPAGEMTVVLGSGWPGVLIHEAIGHGLEGDFNRK
                     KTSAFSDLMGEKVASSICTVIDDGTWSEQRGSLNIDDEGEPTQRTILIENGRLCNYMQ
                     DKLNARLMGAQTTGNGRRESYAAQTIPRMTNTFMAKGPHGHTEIIESVKSGLYAKNFG
                     GGQVDITSGKFVFSASEAYMIKNGKLDAPVKGAMLIGDGPSILHQVSMVGNDLALDPG
                     IGSCGKEGQTVPVSVGQPSMKIDKLTVGGTN"
     gene            complement(2621..>3093)
                     /locus_tag="QGH35_03775"
     CDS             complement(2621..>3093)
                     /locus_tag="QGH35_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HJL81685.1"
                     /translation="YDKIHLFDVVVNEKIYKESSFIEPGSTPVSIQTPWFKLGLSICY
                     DVRFPELYRSKAFNEVDLIVVPAAFTEETGAAHWETLLTSRAIENLSYVLAAAQWGEH
                     YGDRRTYGHSMIINPWGERVNALPSGNGVVIAEIDLELIAEIRENFPALVHRKI"
BASE COUNT          850 a          698 c          593 g          952 t
ORIGIN      
        1 gtgtagtcgt aatccatctc catagagcca ttttctgccg caatggcagt acaactaatg
       61 gtatgtccag ttgattctgt taaaccaaca aaaccatgcg agttagcata aactgttcgg
      121 ttttcatagg tagcaacgtt agctccttcc gtgttctcaa ctttggaatc ggtatccaaa
      181 gcagcttgtt cgcattccag tgacattgtt tgtgcctgct ctacattaag ctcccatggg
      241 tggtacagtt tcagatccat gggtttttgt gccattaaat gagcgtcagc caaaccatta
      301 aattgatcac gggcagttaa attagccagg ctgaaagctt tttcagtagt cgttatgaga
      361 gcattttgtg ataaatcgtt ggtcgttgcc gatccaatcg attgattggc atagacggta
      421 acactgaagg cagatgaaga atagtgttct atagtttcaa tcgattcatt ctgtgcctgt
      481 actgagaagc ctttgccatt tgaacaactt gcctctgttt gatcagcacc aagtttattg
      541 gcatgctcaa gagcgatctc tatacttgtt tctagtattt tattattcat atttattgag
      601 aaatttcgtt gagatatttt aacttactta gacaccacaa ataagtcatt gtagaattat
      661 gcaatgaata ttgacgtagc catcataatg ggttcaaaat ctgactggga cattatgtcc
      721 cattctgcaa acatcctaac ccaatttgac attgcacatg agtccaaggt gatctcggca
      781 catagaacac cggtactgct cgatgaatat tgcagtgaag cccaacaaaa aggtgtcaaa
      841 atcttcatta ccggagcagg acttgcagca gcattgcctg gagtagtcgc agcaaagact
      901 accttaccag tcatcggtgt tcctttggag gcaagttcac tggacggtat ggatgcatta
      961 ctctctattg tccagatgcc gccagggatc cctgttggaa ccatgactat aggtaaagcc
     1021 ggtgcaatca atgcggcatt gtatgcagcc gcaatcctgg gcttgcaaga cgaaaatatc
     1081 agagagaaaa tccagtccta tagagacgaa caagcgcaaa aaataatcga tactaatcta
     1141 gaataattct ctagttggtt ccaccaacag ttaatttatc tattttcatt gatggctgcc
     1201 caacgctgac cgggacagtt tgtccttctt ttccgcaact accaatgcca ggatctaatg
     1261 ctaagtcgtt tccaaccatc gatacttgat gaagaattga tggcccatca cctatcagca
     1321 ttgctccttt tacaggagca tctagtttgc cattcttgat catgtatgcc tcgctggcag
     1381 aaaatacaaa tttccccgaa gttatatcaa cctgtcctcc accaaaattt tttgcataca
     1441 atcctgattt gactgattca atgatctcag tatgtccgtg cggccctttg gccataaaag
     1501 tattagtcat tcttgggatg gtttgcgcgg cataagattc tctgcgtccg ttaccggttg
     1561 tctgtgctcc catgagcctt gcgttcagtt tatcttgcat gtaattgcat agcctgccat
     1621 tttcaataag gatcgttcgt tgtgttggct caccttcatc atcgatgtta agcgaccctc
     1681 tttgctcact ccaagtccca tcatcgataa cggtacagat tgatgatgcc actttctcac
     1741 ccataaggtc agaaaaggca gatgtttttt ttcgattaaa atctccctcc aagccatgtc
     1801 ctatcgcttc gtgaattaat actcctggcc agcctgaacc taacacaacc gtcatttcac
     1861 ctgccggtgc ctcttttgct tctaggttaa cgagcgcttg ttcaactgct tcttctgcat
     1921 aggtataaat cgctttattg ttgaaaagac tataatcgat acgaccgcca ccgccactgg
     1981 aaccactttc cctgcggcca ttctcttcca caatcacatt cacgctgagc ctaaccatcg
     2041 gcctgatgtc tgtagtcagg caaccttgtt gattgatgat aagtatggtt tgataactcc
     2101 ctgccaaagt agcaacaacc tctttgactc gatgatcttt agccctggca tgcgaatcaa
     2161 ttaattctag gatttttatt ttgttattgt ctgaaagaga acccacagga tccgttgatg
     2221 ggtataaatg ctgcattgtt gtccttggtt caatcttgac agaataattc ttttgcttat
     2281 tcgcaatact ctttgcagct ttaaccgcat ttagaagcga ttctttgtca aacgtttcag
     2341 aataagccaa ccctgacttt tcaccaacaa cagatctaaa tccaacccct tgtttattag
     2401 aaaaaactga ttttttgact tgtgaatcct ccaatgtcca tgattcatag cacatagatt
     2461 gaaaataaat atctcccata tcactgccat tagaaagcag tgttgacagc atcaaggcta
     2521 gatcctgttc cgttaaatta taaggctcca gtaaaattga ttttacttgt gcaagagtgc
     2581 tttcagagtt attcatattg gctaatgttt agctatcaga ttagattttt ctatgcacaa
     2641 gggctggaaa attttctctg atttcagcaa tcaattctaa atcaatctcc gcgatgacaa
     2701 caccattacc tgatggcaag gcattgactc tttcacccca tggattaatg atcattgaat
     2761 gaccataggt ccttctatca ccatagtgct caccccattg ggcagctgcc aatacataac
     2821 taagattttc aattgctctt gaagttagca aagtctccca atgtgccgca cctgtttctt
     2881 cagtgaaggc tgcgggaacc acaatgaggt cgacctcgtt gaatgcttta gatcgataga
     2941 gttctggaaa acggacatca tagcaaatag aaagacccag tttgaaccag ggcgtttgaa
     3001 tacttacagg tgtggaaccg ggttcaataa aacttgattc tttgtaaatt ttttcattga
     3061 cgaccacatc aaaaagatga attttgtcat aaa
//
LOCUS       DASCTH010000178         5931 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000283128, whole genome shotgun sequence.
ACCESSION   DASCTH010000178 DASCTH010000000
VERSION     DASCTH010000178.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000283128"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..2121
                     /locus_tag="QGH35_03780"
     CDS             <1..2121
                     /locus_tag="QGH35_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81686.1"
                     /translation="DYLVTDGEQLQVQYSNSVNIGNDGYLMIAAEARTKEASNRVGFQ
                     AARLYEVNSDYWSDDEAETQRLQPGSDWYINPQRMIWGDQAKDNFGAMYNAELPVGDK
                     RFYSFGGYTVRHADTGCYTRKPNQGNKTWLSSSPSGFVPHIQPTVTDFSMSTGMEGVF
                     GNWSYDVSTTYGTNDFHFNMLSTNASFGPEQIRRYDIGGFVFTQLTNNLDLTTQMGNI
                     DLAVGAETRKESYRIYAGETASYANGQAGTNISGWDAYTTSSDTNGIAYADTFITGVN
                     SNTAGGGCQCFSGFRPSNASATQDANRSSYAAYADAEMDLGAMRVGGALRFENYSDFG
                     STVNFKGTVRYEVSDGIVARAAVSSGFRAPALAQAFQSKVATNFMLDDDPESSTYGQT
                     LAFEVGNFPVDNPFSQALGAKELTPENSFNLSAGLNANLGGIRVSVDAYSVTITDRIV
                     MTGNFSAGDSELGIKIAELLTASGVPEATSGRFFFNGVDTKTSGLDLTAAYSMSLADM
                     ADLDLTFAYNTTTTEITEVRTPSGLADVLNADATLFNNQERRTMESSQPKDNLHLTAN
                     LSKGNWYVKYHIWRVGESLSRGAYKKDHDGDGVNDSPLTLDENAGGTWEEWTRTYKNA
                     AGEDVTDHEWVQVFGNKMITDLEVGYDLGMATISCGANNLFDVMPEKGAQPGSGNNGA
                     FVYDGLSPFGMNGKFVYTKLTLDF"
     gene            2212..3483
                     /locus_tag="QGH35_03785"
     CDS             2212..3483
                     /locus_tag="QGH35_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HJL81687.1"
                     /translation="MNQKLHPHLEQLERSATLAINERSVELEKMGQTIYRFGLGQSPF
                     PVPESIVRALQENAFRKDYLPVEGLLELRKAVANFHQEENELKIKPEGIMVGPGSKEL
                     MFTLQLALEGSTLIPSPCWVSYAPQANILKRQNYYINTHFSNKWRLQPGELISAIKST
                     ISPYLLILNYPCNPEGSTYSSDELEAIANVARDNNVLILSDEIYGQLHHDGEHISIAR
                     FYPEGTIVSSGLSKWCGAGGWRLGHFAFPKNLYWLKDAMASIASETYSCVAAPIQHAA
                     VVAYRDKETVEEYLFHCRRILKTVGQFCSNALASANVNIHSPSGAFYLFPDFSNHSEK
                     LNARGINDSSALCAQLLKNTGVALLPGIEFGRPAKELNARIAYVNFDGGKTLLTSRKI
                     SMEQNLTMDDLGENAMLVKQGIENIIQWINN"
     gene            3485..5173
                     /gene="ggt"
                     /locus_tag="QGH35_03790"
     CDS             3485..5173
                     /gene="ggt"
                     /locus_tag="QGH35_03790"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJL81688.1"
                     /translation="MKKIYLLITLVCTTLNGASPDPVFSENGMVVSTSRQASEIGIEI
                     LQKGGNAVDAACAVGFALAVTSPSNGNIGGGGFMVAHMVDGTTFTLDHREMAPAAAHR
                     DMYLDANGNIIENMSLSTRAASGIPGTVDGLLKAWQDYGSGNISRRELLAPAISLAEK
                     RFELSHFEATRLNNFKDFFLKNDAAAKVFIRKDGNPWKDGDIFKQKDLARTLKRIARE
                     GRDGFYTGKTADLIVKEMKRGNGFITHNDLLNYTSKYRDPVKGDFREYEILSMGPPSS
                     GGAVLIEMLNMLEYAELDSMSWNSSEYIHLLTEIERIAFADRAEHMGDSDFWNVPLTM
                     LLSDEYAAKRFGKIKIDEAAKSKDVYHGDPTGYESKETTHFSVLDKDGNAVGVTTTLN
                     LSFGSGIMVERAGFFLNNEMDDFSSKPGTPNFFGLVGKEANAIEPRKRPLSSMTPTIV
                     VKNGNPFMIIGSPGGSIIITATLQSILNVVVFDMDVQEAVSAPRTHSQWLPDILFYEK
                     RGLSEDVIQNLEQKGHVVKMHPWEYLGKTNAIVVTDEWITGGADTRGENTAVGY"
     gene            5179..5667
                     /locus_tag="QGH35_03795"
     CDS             5179..5667
                     /locus_tag="QGH35_03795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJL81689.1"
                     /translation="MQIENVTPGDIPQILELNQVSQPHLSSLTLERLIELVEMTFHFR
                     VIKENGEIAAFLMGMEEEQSYDSMNYAWISDRYDSFYYIDRIAVAEKYQRQGLGLALY
                     NDGQKIAIEIKKPIMACEVNVKPMNQGSILFHKNFGFEPVGEQDTEGGKKRVQYMIKD
                     FL"
     gene            complement(5651..>5931)
                     /locus_tag="QGH35_03800"
     CDS             complement(5651..>5931)
                     /locus_tag="QGH35_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81690.1"
                     /translation="TIGSPHGIQQEWYVDGNKTLEGKFHNGLYVDHWTWWYPDGSVAL
                     EGDFALGNPKDLWIWITPNGDVQTKEFPSIKWVENQIKEWENFIIKNP"
BASE COUNT         1828 a         1082 c         1270 g         1751 t
ORIGIN      
        1 gattatttag tcacggatgg agaacagctt caagttcaat atagcaatag tgtaaatatt
       61 ggcaatgatg gctatttgat gattgccgcc gaagctcgga ctaaagaagc ctcaaaccgt
      121 gttggttttc aagctgcacg tttgtatgaa gtcaattctg attattggtc agatgatgaa
      181 gctgaaactc aacgtttaca gccaggcagt gattggtaca ttaaccctca aagaatgatt
      241 tggggagacc aagctaaaga caattttggt gctatgtata atgcagaatt gcccgtaggc
      301 gataaacggt tctattcttt tggtggatat acagttcgtc atgctgatac cggttgttat
      361 acacgtaaac caaaccaagg taacaaaacg tggttaagtt caagtccgtc aggatttgtt
      421 cctcacattc aacctacagt aacagacttt tctatgtcta caggaatgga aggggttttt
      481 ggaaactgga gttatgatgt gagtactacg tatggtacga atgattttca tttcaatatg
      541 ttaagtacaa atgcatcatt tggtcccgaa caaattcgta gatatgacat tggtggcttt
      601 gtctttactc agttaaccaa caatctagat ttaacaactc aaatgggaaa tatagattta
      661 gctgttggtg cagaaacacg taaagaaagt tatcgcattt atgctggtga aacagcttca
      721 tacgccaatg gacaagctgg aacaaacatt tcaggttggg atgcatatac tacatcatct
      781 gatacaaatg gtattgctta tgcagataca ttcatcacag gagttaattc aaatactgct
      841 ggcggtggat gtcaatgttt tagtggtttt agaccatcga atgcttcagc aacacaagat
      901 gcaaatagaa gcagctatgc tgcttacgcc gatgctgaaa tggatttagg cgcaatgcgt
      961 gtaggtggtg ccttacggtt tgaaaattac agtgattttg gatcaactgt aaattttaaa
     1021 ggaactgtca gatatgaagt ttctgatggt attgttgcta gagctgcagt tagttcaggc
     1081 tttagagctc ctgctttagc tcaagctttc caatctaaag ttgctacaaa ctttatgttg
     1141 gatgatgatc ctgagtcttc tacatatggc caaacactgg catttgaagt aggtaatttc
     1201 cctgttgata atccattttc acaagcattg ggagcaaaag aactcactcc tgaaaactct
     1261 tttaatttga gtgctggttt aaatgcaaat ctgggtggta ttagagtaag tgttgatgct
     1321 tattcggtta ccattacaga taggattgtt atgacgggta atttctccgc tggcgatagc
     1381 gaactgggta taaaaatcgc agagctgctt acagccagtg gtgtcccaga agcaaccagt
     1441 ggtcggtttt tctttaatgg tgtagataca aaaacgtctg gattagatct aacggctgct
     1501 tattcaatga gtttagctga tatggcggat cttgatctga cttttgctta caacacaacc
     1561 accactgaaa taaccgaagt tagaacacct tctggtttgg cagacgtttt aaatgctgat
     1621 gcaactcttt ttaacaatca agaacgtcgt acaatggaga gctcacagcc aaaagacaat
     1681 cttcatttaa ctgcaaatct ctctaaaggt aactggtatg ttaaatatca tatttggaga
     1741 gttggagaat ccttaagccg tggtgcttat aagaaagacc atgatggcga tggagtaaat
     1801 gacagtcctc tcactttgga tgaaaatgcc ggaggtacat gggaagaatg gacgcgtaca
     1861 tacaaaaacg ctgctggtga agatgtaaca gatcatgaat gggttcaggt tttcggtaac
     1921 aaaatgatta ctgatcttga agttggttat gacctaggaa tggcaacaat ttcctgtggt
     1981 gcaaacaatc tgtttgatgt tatgcctgaa aaaggtgcac aaccaggatc gggtaataat
     2041 ggagcctttg tctatgatgg attatctcca tttggaatga acggtaagtt cgtttacacg
     2101 aaattaacgc tcgatttcta aacctaaacg tttagtaata aaaaagcccc gatttttcgg
     2161 ggctttttta tttatacacg aattaatagt gcttaatttc gctagctttt tatgaatcaa
     2221 aaactccatc cacatcttga acaactggaa cgttctgcca cattggcgat aaatgaacgt
     2281 agtgttgaat tggaaaaaat gggacagacc atttaccgat ttggacttgg acaatcacct
     2341 ttccctgttc ctgaatctat tgttcgtgct ctgcaagaaa atgcatttcg caaagactat
     2401 cttcctgtag agggattatt agaattgaga aaagcagttg ctaactttca tcaagaagaa
     2461 aatgaactca aaattaaacc tgaaggcatc atggtgggtc ctggatcaaa agagttgatg
     2521 tttacgttac aattggcatt agagggatcc actctaattc cgtctccatg ctgggtatct
     2581 tatgcccccc aagccaatat tttaaaaagg cagaattatt atatcaatac ccatttttct
     2641 aataagtggc gactccagcc cggtgagctt atttcagcta taaaaagtac tataagccca
     2701 tatctgctca ttttgaatta cccatgcaat cctgaaggaa gcacatattc ttctgatgaa
     2761 cttgaagcca tagcaaatgt tgctcgtgac aataatgtac ttattctatc agatgagatc
     2821 tatggccagc tccatcatga tggggaacac atatccattg cccgatttta tccggaagga
     2881 actattgtta gttcaggttt atcaaaatgg tgtggagccg gtggttggcg gttaggtcat
     2941 tttgcatttc caaaaaattt gtattggctc aaagatgcaa tggcatctat tgccagtgaa
     3001 acttattcct gtgtcgctgc tcctatccag cacgcagccg tcgttgcata tcgagataaa
     3061 gaaacagtgg aagaatattt atttcattgt cgtcgtattc taaaaacagt tggtcaattt
     3121 tgttcgaatg ctttggcttc tgctaatgta aatatacatt ccccatctgg agcattctac
     3181 ctgttccctg atttctcgaa tcattccgaa aaactgaatg ctagaggaat caatgattcc
     3241 tctgctttat gtgcacagtt attgaagaac acaggggttg ccctgcttcc cgggatagaa
     3301 ttcgggagac ctgcaaaaga attaaatgca cgcatagctt atgtaaattt tgatggtgga
     3361 aaaacactgc taacaagtcg aaagatttca atggagcaga atctaacaat ggatgattta
     3421 ggagaaaatg ctatgctagt taaacaagga atcgaaaaca ttattcaatg gataaataat
     3481 taatatgaag aaaatctatc tactaattac acttgtatgt acaaccctta atggtgcaag
     3541 ccctgatcct gtattttcgg aaaatggtat ggtggtttcc accagccgac aagcatctga
     3601 aattgggatt gaaattctac agaaaggtgg caatgcagtg gacgctgcat gcgctgtagg
     3661 gtttgcactc gctgttactt ccccttctaa cggaaatatc ggaggcggtg ggtttatggt
     3721 tgctcatatg gttgacggaa caacgttcac tctggatcat cgtgaaatgg ctcctgcagc
     3781 tgcccatcgt gatatgtacc ttgatgctaa tggtaatatc attgaaaata tgtcattgag
     3841 tacaagagct gcttccggta ttcccggaac ggtggatgga ttattgaaag cttggcaaga
     3901 ttacggctct ggaaatattt cccgaagaga attactcgca ccggctatat ccctagcaga
     3961 aaaaaggttc gagttaagcc attttgaagc aactcggtta aacaatttta aggatttctt
     4021 tttaaaaaac gatgcagcag caaaggtctt catacgcaag gacggaaatc cttggaaaga
     4081 cggtgatata tttaaacaaa aagatcttgc cagaacattg aagcgaatcg cccgggaagg
     4141 cagggacggt ttttataccg gtaaaacagc agatttaatc gtgaaagaaa tgaaacgagg
     4201 aaatggcttt attacgcaca atgatttatt gaattataca tctaaataca gagatccggt
     4261 aaaaggagat tttcgggaat atgaaatttt atccatgggt ccgccaagtt ccgggggcgc
     4321 agtattaatc gaaatgctga atatgctgga atatgctgaa cttgattcaa tgagttggaa
     4381 ttcttcagaa tacattcatt tattaacgga aattgagcgt atcgcttttg ctgatagagc
     4441 cgaacatatg ggtgactctg atttctggaa tgtcccactt accatgcttc tttctgatga
     4501 gtacgctgct aaacgtttcg gaaaaattaa aatagatgaa gctgcaaaaa gcaaagatgt
     4561 ctaccatggc gatccaacgg gatatgagag taaagaaacc acccattttt ctgttctcga
     4621 taaagatgga aatgctgtgg gagtaaccac cacccttaat ttgagttttg gcagcggaat
     4681 catggttgaa agagctggat tctttttgaa taatgaaatg gatgattttt cttcaaaacc
     4741 aggcacacca aacttttttg gactcgtagg taaagaagcc aacgccattg aaccgagaaa
     4801 acgtccatta agttcaatga ctcccaccat cgtcgtaaaa aacggaaacc catttatgat
     4861 tatcgggtct ccgggaggat ctataatcat caccgcaacc ttacaaagta tattgaatgt
     4921 tgttgttttt gacatggatg ttcaggaggc cgtttcagct cctcgcacac attctcaatg
     4981 gcttccggat attttattct atgaaaaaag aggactgtct gaagatgtaa tccaaaatct
     5041 tgagcaaaaa gggcatgttg ttaaaatgca cccttgggag tatttaggaa aaacgaacgc
     5101 tattgttgtt actgacgaat ggattaccgg tggagcagat acccggggag aaaatacggc
     5161 agtagggtat taacaacgtt gcaaattgaa aatgttacac cgggcgacat tcctcaaatt
     5221 ctggaattaa atcaagtttc tcaaccgcac ctttcttcac tgacactaga gcgattaatt
     5281 gaattggttg aaatgacgtt tcatttccgt gtgataaaag aaaatggtga aattgctgca
     5341 ttcctcatgg gtatggaaga agagcaatct tatgacagta tgaattacgc ttggatttct
     5401 gatcgatacg attcatttta ttatattgac aggattgccg ttgcagaaaa atatcaacga
     5461 cagggattag gattggcctt gtacaatgac ggtcaaaaaa tagctataga aataaaaaag
     5521 cccataatgg cctgcgaagt aaatgttaaa cccatgaatc aagggtccat tttatttcat
     5581 aaaaattttg ggtttgagcc tgttggagaa caggatacgg aaggcggcaa aaaacgggtt
     5641 cagtatatga ttaaggattt tttataataa aattctccca ttctttaatt tggttttcca
     5701 cccatttaat cgaaggaaat tcttttgtct gaacatcacc atttggcgta atccatatcc
     5761 ataaatcctt tggatttcct agtgcaaaat caccttctaa cgccacggat ccatccggat
     5821 accaccacgt ccaatggtca acatacaagc cattatgaaa ttttccctct agagttttat
     5881 taccatccac gtaccattcc tgttggatgc catgaggaga tcctatagta t
//
LOCUS       DASCTH010000179         6485 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000284986, whole genome shotgun sequence.
ACCESSION   DASCTH010000179 DASCTH010000000
VERSION     DASCTH010000179.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000284986"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="QGH35_03805"
     CDS             <1..218
                     /locus_tag="QGH35_03805"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017058640.1"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HJL81691.1"
                     /translation="ELLERIKEQYLEQSVSIDDLDGYSFDLSSWRFNLRMSNTEPLVR
                     LNVESRADRELLMKKTDEILDFIEKFK"
     gene            321..581
                     /locus_tag="QGH35_03810"
     CDS             321..581
                     /locus_tag="QGH35_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HJL81692.1"
                     /translation="MINKVHTLFTSHPQSVNETYTEHFKIASYYGWTMLISGIACLIH
                     AVLPFLFLTTASSNLKTLNKQMKDRIPDYGPKVEKEDQIKAA"
     gene            711..905
                     /locus_tag="QGH35_03815"
     CDS             711..905
                     /locus_tag="QGH35_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81693.1"
                     /translation="MKRIKNIAVSGSFENEGHSVIFIENCANCADKIYVKELSTKTIS
                     RAQAINEQDKLIKLGYIKTN"
     gene            1057..3564
                     /locus_tag="QGH35_03820"
     CDS             1057..3564
                     /locus_tag="QGH35_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81694.1"
                     /translation="MKLTARIGFPTIAKYVLGGLIALTMLPVYFVSFEAIGQSVIEEI
                     IVTARKREENLQDVPISISPFSADKIEDLGLKSLDDIAMFTPSFSMTNAFGRQSGSDR
                     PEMRGITTIVNGIGNASAVAYFVDGIYISGSPQSTELHNLERVEILRGPQAAAFGRGT
                     YMGAVNFVTKKPSEENEGGLTLSTGEDGYFEGIGWISGPINDSTSGYISVGFDSFDGQ
                     YTNTRDGSDLGGEKSTNLTGKLFWTPSDDLDVTFKIGYQETDDGHVPYYLTDRNDNNC
                     CGRDSDHPRAREYYQGTAQWDPSGISLSTDLLDTHGDGTGVRLERTLFTLTVNKQFDE
                     NTTFTSTTGYTEDELAVYQDVTYGGYDPYASYAKYCEPPYSYMSWAAGVCGVYGLSGA
                     FYMLDEGDEREDFSQEFRVTTTLSNGADAIFGVYYYEGEATEGDSYSIKEICPATGRW
                     NSNCSTALVPSAVFPNPFISSYTIEKVENFAVFAGMDWDVNEQWSLGVEVRYAEDEIT
                     VSEDPYSLATPASGCDIRLGLCNDTYKSFTPRFTALYEKNDNTNFYFNIAQGTKPGDF
                     NSAVPEKTDGTLDESFRAVDEEVAWSYEVGVKSILRDGRVNISASVFFLNVEDQQLTQ
                     NIELSSGLIDSILANVGESEVKGIEFEGNFQVSDNFNAGLTFSYTDAEITKRISQDHA
                     DLMGSDGSYAQYNLLGNVAGKSMPRVPKTKYSLFGKYETETANGTFYAIGTFAYESSK
                     YAQEHNLIETGSRGIAGIRAGLIVDNWEYVIWVKNLTDDDTPIDILRYIDKRTGNLGS
                     CASFGGAACGGVSNSPRGFALTLPRQRQIGATVNYRF"
     gene            complement(3740..5050)
                     /locus_tag="QGH35_03825"
     CDS             complement(3740..5050)
                     /locus_tag="QGH35_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81695.1"
                     /translation="MSSNDNTYRNKTNAWYMVILMTVAYMFSFIDRYILGLLIDPIKA
                     DLGLSDTQIGLLLGPAFAIFYATMGLPLGYLADRVKRVWIVAIGVFVWSLATVASGFA
                     QKFSQLFIARMGVGVGEATLSPCAISMITDSFSKKDRGKPIGFYTMGMSLGPAVAYLS
                     GAAVLIWAQSVDLVNLPIIGEISPWQLAFIIVGLPGIVLAVLIAMLREPQRTSHKNQN
                     DEEEIKITEALKFFIEKRSALGSFILPACVMTTIAYSHAWLAPMFERTWGMPPSTYAL
                     INGLLLLTFGPISNNTAGWLADRLVKQGKKDGGLRVVILGAFIFLPTAIVAPLVPNPT
                     LCFVVLAINLVGIAFTSSSALIALMEIVPSNIRGLSTAFYLMCISITGLLLGPTSVGL
                     LNDFVFDAENVRYSVSTIPLVLGVPVLAMIPFMRRSYLKEIEKT"
     gene            complement(5050..5481)
                     /locus_tag="QGH35_03830"
     CDS             complement(5050..5481)
                     /locus_tag="QGH35_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJL81696.1"
                     /translation="MRFQRANFLVSDLDRALILYRDILGMRLDHVEESEDDSYSYDVF
                     EIDKKIQIKFALLSYSNQPRIMALTELGKNNLEKMSFPRRSAIVIEVADVDDVLAQCK
                     ENNFKTYKEEHLVTHDGREGREIGIVDDDGNLTVIYKIEKA"
     gene            complement(5497..5964)
                     /locus_tag="QGH35_03835"
     CDS             complement(5497..5964)
                     /locus_tag="QGH35_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJL81697.1"
                     /translation="MATTTVKRTTLIVRDIEASSKWYEEILDMSLYYDDTITLSGQGL
                     AAGKKGDVTHLKIYKCSDPVIGMIGLLQWLDPELPAPEKIPTKVDYGNPTFVVDTDNV
                     DEVYKKALALETHIHSEPHEWSVRGADGNMINFITLSVFDPDGYFYEINQRLT"
     gene            complement(5967..6266)
                     /locus_tag="QGH35_03840"
     CDS             complement(5967..6266)
                     /locus_tag="QGH35_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018995420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HJL81698.1"
                     /translation="MSKKAYMIVFATIHDRESFVTNYGPAAAKLTEKFGGKYLIRAPG
                     GIPLEGMDSSNPSVVISEWPDKETAEKFWHSEEYSEVKKFREGIADCKVLLIESD"
     gene            complement(6276..>6485)
                     /locus_tag="QGH35_03845"
     CDS             complement(6276..>6485)
                     /locus_tag="QGH35_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81699.1"
                     /translation="PDHKKSYLVSMTQQGNKSLVRVNNYQKTLTGVFVDGFDTSIEEL
                     VKATNYIRKLRENSLANIESSHTKQ"
BASE COUNT         1987 a         1199 c         1303 g         1996 t
ORIGIN      
        1 tagagttact ggagaggatt aaagagcaat atctagaaca gtctgtttca attgatgatt
       61 tagatggtta tagttttgat ttatcaagtt ggcggtttaa tctcagaatg tcaaatactg
      121 aacctctggt tagattaaat gttgagtcta gggctgatag agagttattg atgaaaaaga
      181 ctgatgagat tttagatttt atagagaagt ttaaataaga tttgtattcc taaaatagga
      241 acgtcattga cagttaactg tcatgtgata tagtttatta tatagatata cttttttatt
      301 atattaatag actctagata atgatcaata aggttcacac tcttttcact agtcaccctc
      361 aatctgtgaa tgaaacttat acggagcatt ttaagattgc aagttactat ggttggacca
      421 tgctgatctc aggaatagct tgtcttatcc atgccgtcct accattcctt tttttaacta
      481 ctgcaagttc aaatctcaaa acactgaaca agcaaatgaa agatagaatc cctgattatg
      541 gcccgaaggt agaaaaagag gatcaaataa aagctgcata aagtgatatc aaaaagtgat
      601 caatgctaat cccccaaaaa aggtcttata tttcattccc tcgatactat agggcctttt
      661 tttattttac taataacatt tatattatta cttattaaag aaattactta atgaaaagaa
      721 taaaaaacat agcagtaagc ggatctttcg agaatgaagg acactcagtt atcttcatag
      781 aaaattgtgc gaattgtgct gataaaattt atgtaaaaga actttcaaca aaaacaatct
      841 cccgcgctca agctataaat gaacaggata aacttatcaa acttggatat ataaaaacaa
      901 actaatgaaa agataaagac ttaaccttaa aagaggttta attgatttat gtcaatgatt
      961 ctagaacata ttatctataa attacttaaa tgatgccacc attggctaat aaataattgc
     1021 gtctgtaaat atctgaaata acttggagag aaactaatga aattaacagc aagaattgga
     1081 tttcctacta ttgcaaagta cgtattggga ggattaatag cactgactat gttgccagtt
     1141 tattttgtgt catttgaggc aatagggcaa agcgtaattg aggaaattat agtaactgcc
     1201 cgtaaacgag aagaaaatct acaggatgtc ccaatttcta tctcaccatt ctcagctgat
     1261 aagattgagg atttaggttt gaaaagcctc gatgatattg cgatgtttac acctagtttc
     1321 tctatgacta atgcttttgg taggcaatcg ggttcagatc gacctgaaat gcgtggaatt
     1381 actactatcg tcaatggaat tggtaatgct tcagctgtgg cttacttcgt tgatggtata
     1441 tatattagtg gatcacccca atctacagag ttgcataatc ttgagcgtgt tgagattctt
     1501 cgtggaccac aggcagcagc ctttggaaga ggtacttata tgggtgctgt taattttgta
     1561 accaaaaaac caagtgaaga aaacgaaggt ggattaactt tgagcacagg agaagacggt
     1621 tattttgaag gcatcggttg gatcagtggt ccaatcaatg attcaacatc aggttatata
     1681 agcgtcgggt tcgattcatt tgatggacaa tacaccaaca ccagggatgg ttccgattta
     1741 ggtggtgaga aatcaacaaa tttaactggt aagttgtttt ggactccatc tgatgatttg
     1801 gatgtcactt tcaaaatagg ctaccaagaa accgatgatg gtcatgtacc ttattatcta
     1861 accgatagaa acgacaataa ctgttgtggt cgagattcag atcacccaag agcaagagag
     1921 tactaccaag gtacagctca gtgggatcca agcgggatta gtctgtcaac agatctatta
     1981 gatactcatg gtgatggtac tggagtaaga cttgaaagaa cattattcac cttaaccgtc
     2041 aataagcaat ttgatgaaaa taccaccttt accagcacta ctggatacac agaggatgaa
     2101 cttgctgttt atcaggatgt gacatatggt ggctatgacc cttatgctag ttatgcaaaa
     2161 tattgtgaac ccccatatag ttatatgtct tgggccgcag gtgtatgtgg tgtttatggt
     2221 ttgagtggtg cattttatat gcttgatgaa ggcgatgaac gagaagattt ttcacaagag
     2281 tttcgtgtca ctacaaccct aagtaatggg gctgatgcaa tctttggtgt ttattattat
     2341 gaaggagaag caactgaagg ggatagttat tcaatcaaag agatctgccc tgcaactggt
     2401 cgttggaact caaattgttc tactgcgctc gtgccctcag cagtttttcc gaatccattt
     2461 atctcaagtt acactataga aaaagtagaa aacttcgctg tcttcgctgg tatggactgg
     2521 gatgtaaatg aacaatggtc actgggtgtg gaagtcagat atgcagaaga tgagatcact
     2581 gtttctgaag atccatacag tcttgctaca ccggcatcgg gttgtgatat caggttaggt
     2641 ctctgtaatg acacctataa gagttttaca ccaaggttta cagcgttgta tgagaagaac
     2701 gacaatacca atttttattt caatattgct caaggtacca aacctggaga ttttaattcc
     2761 gcagtgcctg aaaagacaga tggaaccctg gatgagagtt ttcgagctgt tgatgaagaa
     2821 gtcgcttgga gttatgaagt tggtgttaaa tccatactta gagatggacg tgtcaacatt
     2881 agtgcttcgg tattttttct caatgttgaa gaccaacaac tgacgcaaaa tattgaactg
     2941 tcaagtggtt tgatcgattc aattttagcc aatgttggag aaagtgaagt caaaggtatt
     3001 gaatttgagg gtaatttcca agtctcagac aacttcaacg ctgggctcac gttctcttat
     3061 accgatgctg aaattacaaa acgtatcagt caagatcatg ctgatttaat gggcagtgat
     3121 ggttcttatg ctcaatataa tctgttgggt aatgttgcag gtaaaagcat gcctagagtt
     3181 ccaaaaacaa aatacagttt atttggaaaa tatgagactg agactgcaaa tggtaccttt
     3241 tatgccatcg gtacctttgc ttacgaatca agtaaatatg ctcaagagca caatctaata
     3301 gagacaggtt ctcgtggtat tgctggcatt agagctggtc ttatcgtaga taactgggag
     3361 tatgtcattt gggttaaaaa tttaacggat gatgatacac cgattgacat tcttagatat
     3421 attgataaaa gaactgggaa tcttggatct tgtgcttcct ttgggggtgc cgcctgtggt
     3481 ggtgtgtcaa acagcccacg tgggtttgcc ttgaccttac ctcgtcagag acaaattgga
     3541 gcaacggtta attatcgctt ctagtaactg ttataattaa tgcatgatgg ttaaattttt
     3601 agctggttag tctctgttga atagaagaca attgttgttt tcggttttac taatcattac
     3661 ttcgctgtgg gtaaaccaca tgaaggcacc catgaaaata tcttctggaa aagaattatt
     3721 taactgaaag ataattactt tatgtttttt ctatttcttt taaataggat cttctcataa
     3781 aggggatcat ggccaacact gggactccta ggactaaagg aatagtgctc acagagtatc
     3841 ggacattctc agcatcaaag acaaaatcat tgagcaagcc tactgatgtg ggtcctaaaa
     3901 gcaaacctgt tatgctaata cacattaagt aaaatgctgt agaaagacct ctgatattgg
     3961 aaggtacaat ttccatgagt gcaatcaaag cgctcgagga tgtaaaagca atgcccacta
     4021 ggttgattgc aagtaccaca aaacaaagtg ttggatttgg aactaagggt gctacgatgg
     4081 cggtgggtaa aaaaataaat gctccaagta tcacaaccct caaaccacca tctttttttc
     4141 cttgtttcac taatcggtcc gccaaccacc ctgcagtatt attggatata gggccgaagg
     4201 tgagcagtaa taaaccgtta atcaatgcgt aagttgaagg aggcatgccc catgttcttt
     4261 caaacattgg tgccaaccaa gcatgggaat acgcaattgt cgtcattaca catgctggca
     4321 agataaagct gcctaaggcg cttctttttt ctatgaagaa ttttaaagct tcggtgattt
     4381 tgatttcttc ttcatcgttc tgattcttat gactagttct ttgtggctct cttagcattg
     4441 ctatgagaac agccagcaca attccaggta acccgacgat aatgaatgcc aattgccacg
     4501 gtgaaatttc accaataatc ggtaaattaa cgaggtctac cgattgcgcc catatcagaa
     4561 cagcagcgcc tgacaagtaa gcaaccgcag gtcccagtga catacccatg gtataaaatc
     4621 caatgggttt tcctctgtct tttttcgaga agctgtctgt aatcatggag atggcacagg
     4681 ggcttagagt tgcctcacca acaccaactc ccattcgggc aataaacagc tggctgaact
     4741 tttgggcaaa gccagatgca accgtggcta gagaccagac aaacacaccg attgcaacga
     4801 tccaaaccct ttttacacgg tctgcaagat agcccaaagg cagacccata gtggcataga
     4861 aaattgcaaa agcaggacct agtaataaac caatttgtgt gtcgctcaag ccaagatctg
     4921 cttttatggg atcaattaat aatcccaaga tgtagcggtc gataaatgag aacatgtatg
     4981 caaccgtcat taagataacc atataccaag cattggtttt attgcggtat gtattatcgt
     5041 tagacgacac taagctttct cgattttgta gatgacggta agattgccat catcatcgac
     5101 aataccaatt tctctgccct ctctcccatc atgagtcact aaatgctctt ccttgtatgt
     5161 tttaaagtta ttttctttac attgtgctaa cacgtcatca acatcggcaa cttctatcac
     5221 aatcgccgat ctgcggggaa aagacatctt ttctaaattg ttcttgccaa gctctgttaa
     5281 tgccatgatt ctgggctggt tggaataact caaaagggca aacttgattt gaatcttctt
     5341 atcaatttcg aatacatcat aagaatatga atcatcttca gactcttcta catgatcaag
     5401 acgcatgccg aggatatctc tgtacaatat taaagcgcga tctaaatcac tgactaaaaa
     5461 atttgctctt tggaatctca tacattctca atggatctaa gttaatcgtt gattgatttc
     5521 atagaagtat ccatccggat caaacacact cagtgttatg aagttaatca tatttccatc
     5581 ggcacctctg actgaccatt catgaggttc tgaatgaata tgtgtttcca gagctagggc
     5641 tttcttatac acctcgtcaa cattatctgt atccacgaca aaggttggat tgccataatc
     5701 cacttttgtt ggaatttttt ctggagcagg gagttcagga tcaagccact gcaaaagtcc
     5761 aatcattcca atgactggat cactgcattt atatatttta aggtgggtta cgtccccttt
     5821 tttgcctgca gctaaacctt ggccactcaa ggttattgta tcgtcgtaat aaagactcat
     5881 gtctaatatc tcttcgtacc actttgagga tgcctcaatg tctctaacga tcaacgtagt
     5941 tctttttacc gtggttgttg ccatatctag tccgattcaa tgagcaatac tttacaatct
     6001 gctatccctt ctctgaattt ttttacttca ctgtattctt cagaatgcca aaatttttct
     6061 gccgtttctt tgtccggcca ctccgaaata actactgaag gattgctgga atccataccc
     6121 tccaatggaa taccaccagg ggctctgatt aagtatttcc cgccaaattt ctcagtaagt
     6181 ttcgcagccg caggaccata attagttaca aaactttctc tgtcatgaat agttgcaaaa
     6241 acgatcatgt atgctttttt tgacatataa gtatcctatt gtttagtgtg agatgattct
     6301 atgtttgcca atgaattttc tcttagtttt cgaatgtagt tggtcgcttt cacaagttcc
     6361 tcaatggagg tatcaaatcc gtcaacaaat accccagtga gggttttctg gtaattgttc
     6421 actcttacca agcttttatt gccttgttga gtcatggata caagatagga tttcttatga
     6481 tctgg
//
LOCUS       DASCTH010000180         2241 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000285056, whole genome shotgun sequence.
ACCESSION   DASCTH010000180 DASCTH010000000
VERSION     DASCTH010000180.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000285056"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(178..1293)
                     /locus_tag="QGH35_03850"
     CDS             complement(178..1293)
                     /locus_tag="QGH35_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJL81700.1"
                     /translation="MSNNNQTTKKEPFIEIKGISKHFGDFVAVNKVNLKIFKGELFSI
                     LGASGSGKTTLLRVMAGLETPTEGSVIIDGIDMTQMPPYERPVNIMFQNYALFPHMTV
                     FNNIAYGLKKERMPKKEIESKVAQILELVELSEYSQRKPDQLSGGQSQRVALARALVK
                     QPKVLLLDEPMAALDKKLRQNTAFELVNIQDELGVTFIIVTHDQEEAMTLSDRIAVME
                     EGKFLQIGPPNEIYENPRSKFIADFVGNINMFAATILSKQTNKIVVNCPTLGGQHEID
                     FSGSIADDIESITLAIRPEKIMIDNQLSGSESNYIKGIVEDFGYFGNLSIYRVKTSDE
                     NIIQVSRQNRYRSERVISWDDEVYLSWDPSNLLLLTE"
     gene            complement(1315..>2241)
                     /locus_tag="QGH35_03855"
     CDS             complement(1315..>2241)
                     /locus_tag="QGH35_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024739.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HJL81701.1"
                     /translation="ESSEMVEAKLLTGNTGYDIVVHSSMYSKRLFPLKIFQRLDKTKL
                     TNWKLIDPKLMTILDKFDPGNNFMAPYSWGSTGVAYNVDMVLERLPDAPLHSAAMLFD
                     PEVVSKFEDCGVSLLDSPTDVVPQALVYLGYADNTRDPKAIQEAEDLLLSVRPYIKKF
                     TSTTFLNDLPNGDLCVSMSWAGDYATAAARAEEVGADINLAYEVPIEGSLMWFDIIII
                     PDDAPHTENAYLFLDYLLRPEVTADFVNITHYASPIPAANEFIDEKILNNPAIYPTPE
                     IMKRLYLTEVDPPKYQRQKTRVFTRAKTGLKN"
BASE COUNT          621 a          437 c          453 g          730 t
ORIGIN      
        1 gaaacaaggg tgtgaatggt ggttgggcta caagtggatc ggcaagactt attttgaaaa
       61 cgattaagaa aggggtgaga aagaaaagca gtaaccataa gtagggtatg aaaactataa
      121 atttcttcca gagtctttcc ctgaatgtcg catccatgct agctaatact gctgacatta
      181 ttcagtcaat aacaaaaggt tagaagggtc ccatgaaaga taaacttcat cgtcccaact
      241 gatgactctc tctgatctgt atcggttttg tctacttacc tgaataatgt tttcatccga
      301 agtttttact cggtaaattg agaggttgcc aaagtaaccg aaatcttcaa cgatgccttt
      361 tatataattg ctttcagaac cagacagttg attatcaatc ataatttttt caggtcgaat
      421 cgcgagagtg atcgattcta tatcgtcggc aatacttccg ctaaaatcga tttcatgttg
      481 accacccaat gtaggacaat tgaccacaat tttattagtt tgctttgaga ggattgttgc
      541 agcaaacata ttgatgttgc caacaaaatc agcaataaat tttgatcttg ggttttcata
      601 aatttcattg ggcgggccaa tttgcagaaa ttttccttcc tccatcacag cgatccggtc
      661 tgacaaggtc atagcttctt cctggtcgtg ggttacgatg atgaaagtga ctccaagttc
      721 atcttgaatg ttgactagtt cgaatgctgt attttgcctc aattttttat ccagtgctgc
      781 catgggttca tcaaggagaa gcacttttgg ttgtttgacg agagctctgg caagagcaac
      841 ccgttgtgac tgtcctcctg aaagctgatc tggtttacgt tggctgtatt cggacaattc
      901 caccagttca agaatttgtg ctacctttga ttcaatttct ttcttgggca ttctttcttt
      961 ttttagcccg taagcaatgt tgttaaatac agtcatgtga gggaacaagg catagttctg
     1021 aaacataata tttacaggac gttcatacgg tggcatttgt gtcatatcga taccatcgat
     1081 gattacggaa ccttcagtag gtgtttccaa gcctgccatg actctgagta aagttgtttt
     1141 acctgatccg gatgcaccca ggatagaaaa taactcacct ttgaagattt tcaggttaac
     1201 tttgttgact gctacgaagt caccaaagtg cttgctgatt cctttgattt ctataaaagg
     1261 ttctttttta gttgtttgat tattgttgct catttcaaaa taatacagtt tttatcaatt
     1321 ttttagtcca gtttttgctc tggtaaaaac cctggttttt tggcgttggt attttggagg
     1381 atcaacttct gtaagataca aacgcttcat tatctctgga gttgggtaaa ttgcagggtt
     1441 attcaaaatt ttttcatcaa taaattcatt cgctgcaggt atgggacttg cgtagtgagt
     1501 tatatttaca aagtctgctg ttacttcagg tcttaatagg taatcgagga ataaataggc
     1561 attctcggta tggggtgcat catccgggat gatgataata tcaaaccaca tcagtgaacc
     1621 ctctattggt acttcatagg ctagattgat atcagcgccc acttcttcag ctcttgccgc
     1681 agctgttgca taatctcccg cccaactcat agacacacat agatcgccat tgggtaaatc
     1741 attgagaaag gttgtagagg taaatttttt gatgtacgga cgcacactca acaacagatc
     1801 ctctgcctct tgtatcgcct ttggatctct ggtgttatca gcataaccta aatagaccaa
     1861 tgcttgtggt acgacatctg tcggagagtc aagaagactt actccacaat cttcgaattt
     1921 agaaaccact tcaggatcaa acagcattgc cgcactatga agtggggcat ctggcaatct
     1981 ttccaaaacc atatcaacgt tatacgcgac gcctgtagat ccccaactgt aaggagccat
     2041 gaaattgtta cctggatcaa atttatctaa tattgtcatc aatttcgggt ctataagttt
     2101 ccagttggtt aatttagttt tatccaatct ttgaaaaatt ttcagaggaa aaagacgttt
     2161 tgagtacatg ctgctgtgca ctacaatgtc ataaccggtg ttgccagtta atagttttgc
     2221 ctcaaccatc tcgctggatt c
//
LOCUS       DASCTH010000181         1738 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000287207, whole genome shotgun sequence.
ACCESSION   DASCTH010000181 DASCTH010000000
VERSION     DASCTH010000181.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000287207"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="QGH35_03860"
     CDS             complement(<1..597)
                     /locus_tag="QGH35_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012339.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HJL81702.1"
                     /translation="MPNSKNNEYKIAFVPSGKNGCFPEGTKILDAARILGVDLDSVCG
                     GRAICGRCQIELSEGEHAKHRISSKLENLSSITEAEKKYAKQKSLKANRRLGCQSKIL
                     GDIVVDVPPESQLHQQLVRKEFEATEITINPSVHLYYIELAEAISTKRANLLNQIKTA
                     LEDQWSLTNLNFIVEDWKQILAILKEGQGTCTVAVRNKQ"
     gene            complement(607..1494)
                     /locus_tag="QGH35_03865"
     CDS             complement(607..1494)
                     /locus_tag="QGH35_03865"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007224870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HJL81703.1"
                     /translation="MSTTETVLSSKTKEIRIGSDQPFVVIGERINPTGRKLLAEEMKS
                     GNLERVKTDAIAQIAAGAQMLDVNAGIPLADEPMILAESIKLIQSVTDVPLCIDSSIV
                     DALAAGLEAYEGKALLNSVTGEEERLERVLPLVKQYGAAVIGISNDDTGISEDINVRF
                     EVAKKIVERASDHGIPKEDVIIDPLVMPIGALNQAGRQVLELVKRLREDLKVNTTCGA
                     SNLSFGLPNRHGLNAAFIAMAISSGMPCAITNPLDKEIMQSIRGANVVMGHDPECAEW
                     INNYREPREKQSRRRRKRS"
     gene            complement(1499..>1738)
                     /locus_tag="QGH35_03870"
     CDS             complement(1499..>1738)
                     /locus_tag="QGH35_03870"
                     /inference="COORDINATES: protein motif:HMM:NF025142.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HJL81704.1"
                     /translation="GREKYKKQLDPKFAIAIDRAAMRAGKGSSEAYMQEWRRETQRLD
                     GDPEELIEILVSQLENEFPDDILEKTIKSSGIKPK"
BASE COUNT          424 a          383 c          312 g          619 t
ORIGIN      
        1 ctgtttattt ctgactgcaa ctgtacatgt tccttggcct tcttttaaga tcgctagaat
       61 ttgtttccaa tcttcaacta taaaatttag atttgtcagt gaccactgat cctccagtgc
      121 tgtcttaatt tgattgagca gattggctct tttagtggaa atagcttctg ctaattctat
      181 gtaatataaa tggacggatg ggttgatggt aatttctgta gcttcaaatt cttttctgac
      241 aagctgctga tgtagttggc tttcaggtgg cacatctacc acaatatctc ctaggatctt
      301 tgactgacaa cctaaccttc tattggcctt caacgatttc tgcttggcat attttttttc
      361 agcttcagtg attgatgaaa gattttctag ctttgatgaa atgcgatgtt ttgcgtgttc
      421 tccctcagat aattctattt gacagcgccc acaaatagct cttcctccac aaacagagtc
      481 taagtccacc cccagtattc ttgcagcatc aaggattttt gtgccttcag ggaagcatcc
      541 attttttccg gaaggtacaa atgcaatttt gtactcatta ttctttgagt taggcatgaa
      601 aaatttttag gatctctttc ttctgcgtct ggattgcttt tctctgggtt cgcggtaatt
      661 attgatccat tcagcacatt ctggatcatg ccccataacg acattcgccc cccttattga
      721 ttgcatgatt tctttatcta atgggttagt aatagcacaa ggcattcctg aactgattgc
      781 cattgctatg aatgcggcat ttaaaccatg acgattaggt agaccaaaac ttaaattcga
      841 agcaccacat gtggtgttta cctttaaatc ttctctgagt cttttgacta gttctaaaac
      901 ttgtctgcct gcctgattca gtgctccaat tggcatcact aatggatcta tgatcacgtc
      961 ttctttgggt atgccgtgat cgctcgcacg ttcgactatt tttttagcaa cctcaaaacg
     1021 gacattaata tcttctgaga tgcctgtgtc atcattggat ataccaatta cagcagcacc
     1081 atactgcttg actaagggca atactctttc tagtcgctcc tcttctcccg tgacagaatt
     1141 cagcagcgct ttaccttcat atgcctcaag tcctgctgct aacgcatcta caattgagga
     1201 atcaatacac agtgggacat ccgttacact ttgaatcaat ttaattgatt ctgcgagaat
     1261 catgggctca tctgctaatg gaattccggc attaacatca agcatctgag cacctgctgc
     1321 tatttgtgca atagcatcag ttttgaccct ttctaaattg cctgatttca tttcctcagc
     1381 aagtaacttt ctcccggtag gattgatacg ttcaccaatg acgacaaaag gctgatcaga
     1441 gcctattctg atttctttgg ttttagaact taagactgtt tctgttgtag acatggtttt
     1501 attttggctt tattccacta gatttgatgg ttttttcaag aatatcatca ggaaattcat
     1561 tttcaagttg actgacaagt atttctatta attcttctgg atccccatca agacgctgtg
     1621 tttctcttct ccattcttgc atataagcct cactggagcc ttttcctgcc cgcatggcgg
     1681 ctctgtctat agcaattgca aattttggat ccagttgttt tttatatttt tctcgacc
//
LOCUS       DASCTH010000182         1703 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000287647, whole genome shotgun sequence.
ACCESSION   DASCTH010000182 DASCTH010000000
VERSION     DASCTH010000182.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000287647"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="QGH35_03875"
     CDS             <1..504
                     /locus_tag="QGH35_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81705.1"
                     /translation="IGDWATHLMKIRDLQNKTGGITEFIPLPFVSMESPMYRRGQARP
                     GPTFREVLLMHAVSRLYLHPIIKNIQTSWVKLGKNAALSCLNAGANDLGGTLMDESIS
                     KSAGGIHGQEFYPREMESFIQDMKRIPVLRDTLYNPINQTSLPNQSSLMLSDKIHSLV
                     DSAYPVI"
     gene            658..1107
                     /locus_tag="QGH35_03880"
     CDS             658..1107
                     /locus_tag="QGH35_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012241.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HJL81706.1"
                     /translation="MVNTIFDSVWRDISPFAVGFDRTFDTLSLLANSKSQNANYPPYN
                     IRKLSDDQYTIELAVAGFDESDVDVEVTGENLTIKGNRPKEAADGLMHQGLAARNFER
                     KFILSDDMVVKGAALSNGMLYIGMEKIIPEHRKPKKISLTSKKKLLG"
     gene            complement(1156..1548)
                     /locus_tag="QGH35_03885"
     CDS             complement(1156..1548)
                     /locus_tag="QGH35_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81707.1"
                     /translation="MNYKAWFRFLIIFPMVLVIAAVALDFSYPFPESVSEYYALLAVS
                     GFSRIYYAYLLAALAGFIADMCLFFFVKNSREIWLILISVFYVLGALMPELTIMTPVS
                     IVMVQIASLLGGIKIAVAYYSPHIRDLF"
     gene            complement(1552..1689)
                     /locus_tag="QGH35_03890"
     CDS             complement(1552..1689)
                     /locus_tag="QGH35_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014019.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HJL81708.1"
                     /translation="MDRMKKNAKEVGGNAVISMQFTTSVLMGGAAEMMAYGTAVVIEE
                     E"
BASE COUNT          568 a          340 c          317 g          478 t
ORIGIN      
        1 atcggtgatt gggccactca cctgatgaag atccgagatc tgcaaaacaa aacggggggt
       61 attactgaat ttataccact accctttgtc agtatggaat ctccaatgta ccgaagaggt
      121 caggcacgtc ctggtccgac attcagagaa gtcctattaa tgcatgcagt ctctagattg
      181 tacttgcatc ccataataaa aaatattcaa accagctggg ttaaattggg aaaaaatgct
      241 gccttatctt gtcttaatgc aggggctaat gacctaggtg gaacacttat ggatgaatcc
      301 atttctaaat ccgcaggagg cattcatgga caagaatttt acccaagaga aatggaatct
      361 tttatccagg atatgaagag aattcctgta ctaagagaca cattgtataa cccaataaat
      421 caaacatccc ttcccaatca atcttctttg atgctttccg ataagataca ttcattggta
      481 gatagtgctt acccagtaat ttaattattt gcctgctatc tctccaatat attttcagta
      541 ataatttcct ttttcatgac ttgaagtcca tcagattatc catatatcta gaatgtggat
      601 gccattaatt gggtctacaa atgaatgatt aacttgctta acaaaaagga gaaaattatg
      661 gttaatacaa tatttgattc agtatggaga gacatctcac cctttgctgt aggttttgat
      721 agaacctttg ataccctatc actgctggca aactcaaagt ctcaaaatgc caattatcca
      781 ccttataaca tcagaaaatt gtctgatgat cagtacacga tcgaattagc tgttgctgga
      841 tttgatgaaa gtgatgttga tgttgaggtt accggagaaa acttgacgat caaaggtaat
      901 cgacctaagg aagctgcaga tggattgatg catcaagggc ttgcggctag aaattttgaa
      961 cgaaaattta ttctatctga tgatatggtc gttaaaggtg cagccttgtc caatggaatg
     1021 ctatacattg gcatggagaa aattattcca gaacatcgaa aacccaaaaa aatatccctc
     1081 acttcaaaaa agaagttatt gggataataa ggaaaagggg gctataacta gccccctttt
     1141 tataagtcta ggcactcaaa acaagtctct tatatgggga gaataatagg caactgctat
     1201 ttttattcct cccagtaatg aagcgatctg taccatcaca atggaaactg gagtcataat
     1261 ggtaagttca ggcatcagtg ctccaagtac ataaaatact gaaattaaaa ttagccaaat
     1321 ctcccttgag ttcttaacaa agaaaaagag gcacatatct gctataaaac cagcaagagc
     1381 agccaatagg taagcataat aaattctact gaagccactc actgcgagta aggcataata
     1441 ttcgctaact gactcaggaa acggataact gaaatcaaga gcaacggcag caataaccaa
     1501 taccattgga aaaatgatca gaaaacggaa ccaagcctta tagttcattg ttcactcctc
     1561 ctcgatgaca actgcagtgc cataagccat catctcagct gctccaccca ttagaactga
     1621 tgtagtgaac tgcatactga taacggcatt gccaccaact tctttagcat tttttttcat
     1681 tctatccaag gcttgttctc ggc
//
LOCUS       DASCTH010000183         1595 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000289650, whole genome shotgun sequence.
ACCESSION   DASCTH010000183 DASCTH010000000
VERSION     DASCTH010000183.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000289650"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="QGH35_03895"
     CDS             <1..470
                     /locus_tag="QGH35_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJL81709.1"
                     /translation="DFPDQSFDIVASSGAITQISDKANIFRECFRVLKPGGYFTCYDW
                     TKSEEPLSEDMFYWFKMEGLTYALETLEEYKVHLQKIGYIDVIIKDASDWYRNQVRRE
                     YELMKGSLYPRMVDLLGQKDADHFVEDWRAMLVVCEKGEIRQGYCRGRRPLNE"
     gene            463..816
                     /locus_tag="QGH35_03900"
     CDS             463..816
                     /locus_tag="QGH35_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HJL81710.1"
                     /translation="MSENKNMENLFSYGTLQQENVQLKNFGHTLEGSKDTLQKYCLED
                     IEITDELVLKTSEKKFHPILFLTGNEEDEVLGTVFKITSSELLKADSYEVDDYHRVQV
                     ILKSGTKSWVYVGNR"
     gene            847..>1595
                     /locus_tag="QGH35_03905"
     CDS             847..>1595
                     /locus_tag="QGH35_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJL81711.1"
                     /translation="MKFLKYFLAIIFLSAFLIFILLRIPAVEDKVLTSALNNLLNQEV
                     DLYDNSLTALVCGSRSPLPHPRRAETCVLINAGGSYYVIDVGDGSVSNLRNYGVNLGK
                     IKAVLLTHLHSDHISDLADIHMGTWIAQSRTKRLDVYGPSGVELVTKGFEDTYKLDYK
                     YRNEHHGDEVAPINIAGFDPHPVDLLNPVVINENGLKVTAFKVSHDPVKPALGYRFDF
                     KGRSIVISGDTSYSENLITHAKDADVLFHEA"
BASE COUNT          511 a          245 c          332 g          507 t
ORIGIN      
        1 ttgattttcc tgatcagtct tttgatattg ttgcctcatc aggtgcgatc actcaaatat
       61 cggataaggc aaatatcttc agggaatgtt ttagagtgct taaacctggc ggttacttca
      121 catgttatga ctggaccaag tcagaagaac cattgtcaga agacatgttt tactggttca
      181 aaatggaagg tcttacctat gcacttgaaa cattagaaga atataaagtc cacctccaga
      241 agattggtta tattgacgtc ataattaagg atgcatctga ctggtatcgt aatcaggttc
      301 gtagagaata tgaacttatg aaaggaagcc tctatcctcg tatggttgac cttttaggtc
      361 agaaagatgc cgatcatttt gttgaggatt ggcgagcaat gttggtggtc tgtgagaagg
      421 gagagattcg tcagggatac tgcagggggc gacgaccgtt aaatgagtga gaataagaat
      481 atggaaaacc tattttctta cggaactctg caacaagaaa atgttcagtt aaaaaatttt
      541 ggacacactc ttgaaggttc taaagacaca cttcaaaaat attgcttgga ggacatagag
      601 attacagatg aattagttct taaaacaagt gagaaaaagt ttcatcccat tcttttttta
      661 actggcaatg aagaagatga agtgcttggc acagttttta aaattacctc atctgaacta
      721 ctgaaagcag atagttatga agttgatgat tatcatagag tccaagtaat attaaaatca
      781 ggtacaaaaa gctgggtata tgttggcaat agatgaataa attttttttt caaatgaggc
      841 agtaaaatga aatttcttaa atattttcta gcaattatat tcttatcagc ttttctaatt
      901 ttcatattat taagaatccc ggctgtcgaa gataaggtgt tgacatcagc tttaaataat
      961 ttactaaatc aagaagttga tctttatgac aactctttaa cagctctagt ttgcggatct
     1021 cgttctccgc ttcctcatcc aagaagagcc gagacatgcg ttttaattaa tgctggaggt
     1081 agttattatg tcatagatgt tggagatgga agtgtaagta atctcaggaa ctatggagtt
     1141 aatctaggta agattaaagc agtattgctt actcatcttc attcagacca tatctctgat
     1201 ctagcagata ttcacatggg aacctggatc gctcagtcta ggactaagag attagatgtt
     1261 tatggtccat cgggcgtcga gcttgtgact aaaggttttg aggatacata taaactcgat
     1321 tataaatatc ggaatgaaca tcatggtgat gaggttgcgc caattaatat tgcaggattt
     1381 gatcctcatc cagttgattt attaaaccct gttgttatta atgaaaatgg attaaaggtt
     1441 actgcattta aagtgtccca tgatccagta aaaccagcat tagggtatcg ttttgatttc
     1501 aaaggcagat ctattgttat tagtggagat acttcatata gcgaaaactt aattactcat
     1561 gcaaaagatg cggatgtatt atttcatgag gctca
//
LOCUS       DASCTH010000184         2176 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000290582, whole genome shotgun sequence.
ACCESSION   DASCTH010000184 DASCTH010000000
VERSION     DASCTH010000184.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000290582"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="QGH35_03910"
     CDS             <1..698
                     /locus_tag="QGH35_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81712.1"
                     /translation="LEHQITLGSGGPESADMEQMIDLILQYQVYYDANLQMYGGVNLR
                     KELGVDMIWTNEAKYFTPYAQTLLEKRGDPPPESDVAEYAQRVLELNKLYEAGGENLL
                     IVGTDEPVYTTLLPGFAFHRELLAMVHAGLPNITVLKAATINGANALGVADKLGSIEP
                     GKLADLLVIKGNPFEDIKATRNIELVIKGGVIYYPEILLKSAEGMIGPQGPDDHDDWE
                     LHIEPLRPANLHQ"
     gene            complement(734..>2176)
                     /locus_tag="QGH35_03915"
     CDS             complement(734..>2176)
                     /locus_tag="QGH35_03915"
                     /inference="COORDINATES: protein motif:HMM:NF039587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HJL81713.1"
                     /translation="RRPFFAENQGLFTINSHDYGLINTRRIGSSPDYNCDEYSVANGG
                     SDVLQDSCENEQKANNDIDTALKITQLGENTDVGFFAAFEDDETFSKGREFYALRALR
                     RLNQHKLGYLATHVERDALDRSATVHAFDYENQATEALTLNSLLMYSDTTDESGFGMR
                     FGMSYEPDKYWNTGISYYRSDDDLNVNDMGYQTRNNLSKLRTFTKYTQTDFSQTSKII
                     AREYRLAFSKRADTHGLQLSQRAELEFKQTFKDTSGFEIEFGQSSSGNDDLLTRGSTL
                     APFVKFGQGRGFEFTYYGRQNERWSYWMHLERERDSYQSFTISENAIALGGNYYPSDD
                     LKFGIQIAKANSKNWLNWLGDNRIGVYPLDYFSIRANATWFPKEKHELRIKFQTTVME
                     AETGVEWLADSVGHLNPTNTSATGFDLGQLAFQIRYKYEISPLSHFYAVYTRGGRHYE
                     DDDVSVSKIIRGTWDNPEEDRFTLKLRVKF"
BASE COUNT          656 a          474 c          404 g          642 t
ORIGIN      
        1 gtcttgagca tcagattaca ttgggtagtg gtggcccaga aagcgctgat atggagcaga
       61 tgattgatct gattctgcaa taccaggtgt attatgacgc taacttgcag atgtacggtg
      121 gagtcaatct acgaaaagaa cttggtgtag acatgatttg gaccaatgag gcaaaatact
      181 tcacccccta cgcacaaaca ttacttgaga aacgaggtga cccaccacca gaatcagacg
      241 tagctgaata tgcacagcga gtgcttgaat tgaataaact gtatgaagct ggcggtgaga
      301 atcttctcat cgtaggcacc gatgagcctg tctatacaac gttattgcca ggatttgcat
      361 ttcatcgtga actcttggcg atggtacacg caggtttacc aaatattaca gtactgaaag
      421 cagcaacaat taacggtgca aatgcgcttg gtgttgcaga caagcttgga tccatagaac
      481 caggaaaact ggctgacctc ctcgtcatca agggtaatcc atttgaagat attaaagcta
      541 cacggaatat cgaattagta attaaaggcg gtgtaatcta ttacccagag atactgctca
      601 agtcagctga aggtatgatc ggaccccaag gacccgatga tcatgatgat tgggaacttc
      661 atattgaacc tcttagacca gcaaacttac atcagtaact tttaatttac atccctaaaa
      721 caaataggca ctcttaaaac ttaactctta actttaaagt aaatctgtct tcttcagggt
      781 tgtcccaagt gcctctaatt attttactca cacttacatc atcatcttcg taatgcctgc
      841 ctcctcgggt ataaactgca tagaaatgag acagtgggct gatttcatat ttgtacctaa
      901 tctggaaggc caactgtcca agatcaaatc cagtagctga tgtattggta ggattcaagt
      961 gacctacact gtcagctaac cattcgactc cagtctcggc ttccataaca gtagtttgaa
     1021 atttaattcg cagttcgtgt ttttcttttg gaaaccaagt agcattggcc cttatagaga
     1081 aataatcaag aggatagacc ccaatcctgt tgtctcccaa ccaattgagc caatttttag
     1141 aattagcctt cgctatctgt atgccaaatt taagatcatc tgaagggtaa taatttccac
     1201 ccagcgctat ggcattttca cttatagtaa atgattggta tgaatccctt tctctttcaa
     1261 gatgcatcca ataactccaa cgctcatttt gtcttccata gtaagtaaat tcgaaccccc
     1321 taccctgacc gaatttaacg aagggtgcca atgtactgcc tcgggtcaat aggtcatcgt
     1381 ttccagaaga actctgtcca aattcaatct caaagccaga tgtatcctta aacgtctgtt
     1441 taaattcgag ttcggctctt tgtgataatt gaagcccatg agtatcggct ctttttgaaa
     1501 aagcaagtct atactcacgt gctataattt ttgaagtctg tgaaaaatcc gtttgtgtat
     1561 actttgtaaa agttctcaac ttactcaaat tattccgggt ctgataaccc atatcattga
     1621 cgtttaggtc atcatcagaa cggtaataag aaatacccgt attccagtac ttatcgggtt
     1681 cgtagctcat accaaaacgc atcccaaaac cactttcatc agtagtatct gagtacatca
     1741 ataaactatt taatgtcaga gcttccgttg cttgattttc ataatcaaaa gcatggacgg
     1801 tagcacttct atccaaggca tctcgttcaa cgtgcgttgc aagatacccc aacttatgct
     1861 gatttaatcg cctcaaggct ctgagagcat aaaattcacg tcctttacta aatgtttcat
     1921 catcctcaaa agcagcaaaa aaaccaacat cagtattttc tcccagttgg gtaatcttta
     1981 aagctgtgtc aatgtcattg tttgcctttt gttcattctc acaggaatct tgtaatacat
     2041 cagaaccacc atttgcaact gagtattcat cgcaattata gtcaggagaa ctgccaattc
     2101 tgcgggtatt gataagacca tagtcatgac tattaatagt aaacaatcct tggttttcag
     2161 cgaaaaatgg ccttct
//
LOCUS       DASCTH010000185         1831 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000291177, whole genome shotgun sequence.
ACCESSION   DASCTH010000185 DASCTH010000000
VERSION     DASCTH010000185.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000291177"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="QGH35_03920"
     CDS             complement(<1..447)
                     /locus_tag="QGH35_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012428.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJL81714.1"
                     /translation="MDAKINNKDKALINRRRKHLGNAEVFFYQTPLHITKSKDVWLFD
                     AEDNKYLDVYNNVAHIGHANQDVIDAITQQSKTLNTSTRYLHESIVCLAEQLTATMPE
                     DLNVCYFTCSGSEANDLALQIARAYSKNQGCIISENAYHGNTTAVFQ"
     gene            complement(428..1513)
                     /locus_tag="QGH35_03925"
     CDS             complement(428..1513)
                     /locus_tag="QGH35_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013775.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HJL81715.1"
                     /translation="MSNNIFSFPTLESPAPRLSLDDAQQAASTWFSDIVDLEPLDSER
                     DQNFLATNKKQQQYVLKIANSKESFDVLDFQNQALNHLLKSSPSLLLPRVCQDLSGKE
                     ISSIVVNNNQHYVRLLSFIKGVPLEDSTDQDRPDVLHRTMGGFLAKLGKGLEGFSHPS
                     SGHELLWDVKQAGFLYHLLSHIKDKHKRDIARASLERFTKNTEPKIQQLRSQVIHNDM
                     NQANVILDFNQDGLVLGMIDFGDMVYAPLVNDLAVAAAYQTIATEDLTQGTVHLLSGY
                     QQEYPLNENELALLPALITNRITMTVIIGEWRGAQHPENKQYILGGVEKSWRILEELC
                     RLNLESAAETFIKKAQSINLEHGRKNK"
     gene            complement(1552..>1831)
                     /locus_tag="QGH35_03930"
     CDS             complement(1552..>1831)
                     /locus_tag="QGH35_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HJL81716.1"
                     /translation="WDDLKETLMPILKDQLGATYLPWLAANNQAIINGDDQLSVNIKG
                     ENFSHSVSSAQKYHLKSFTMLLKEYQTIPNRHLLDSVLKEAGCVDYFE"
BASE COUNT          482 a          361 c          365 g          623 t
ORIGIN      
        1 ttgaaacact gccgtggtat tgccatggta ggcattttca gaaataatac agccttgatt
       61 ttttgaatag gctctagcaa tctgcaatgc caaatcatta gcctcacttc cactacatgt
      121 aaaataacaa acattgagat cctctggcat ggtcgctgtg agctgttctg ccaaacacac
      181 aatactttca tgcaaataac gggtgctggt atttagggtt tttgactgtt gtgttatcgc
      241 atcgatcaca tcttggttcg catgcccaat gtgggcaaca ttattgtaaa cgtctaaata
      301 tttattatct tctgcgtcaa acaaccaaac atccttgctt ttagtgatgt gcaggggtgt
      361 ttggtagaaa aaaacctcag catttcccaa atgcttccgt cttctgttga ttaaggcttt
      421 gtctttgtta tttatttttg cgtccatgtt ccagattgat agattgtgcc ttttttataa
      481 aggtttctgc cgcgctctct aaattaagcc tgcataactc ttcgaggatt ctccatgatt
      541 tttctactcc acctagaata tattgtttgt tttctggatg ttgagcacct ctccattcac
      601 caatgattac tgtcatggta attctgttgg taattaatgc tggcagcaat gctagttcgt
      661 tttcattcaa tgggtattct tgttggtagc ctgataacaa atgaaccgtt ccttgagtta
      721 gatcctcagt agctatggtc tggtaagctg cggcaaccgc gaggtcatta actaagggtg
      781 cataaaccat atctccaaag tcgatcatcc ctagaactaa accatcttga ttgaaatcca
      841 aaataacatt ggcttggttc atgtcgttgt gaatgacctg tgagcgtagt tgctgtattt
      901 ttggctccgt gttttttgta aaacgctcta ggctagctct tgcaatgtct cttttgtgtt
      961 tatctttgat gtgtgaaagc aggtgatata gaaagcctgc ttgtttgaca tcccagagca
     1021 actcatggcc tgagctgggg tgtgaaaaac cctccaatcc tttgcccagt ttggcgagaa
     1081 atcctcccat cgttctatgt aaaacatctg gacggtcttg atcggtgcta tcctccaaag
     1141 gaacgccctt gataaaacta agaagccgaa catagtgttg attgttgttt accacaatgc
     1201 tagatatttc ttttccgctc aggtcctgac acactcttgg caacaacaaa gaaggtgaag
     1261 acttcaataa atgatttagt gcttggtttt gaaaatcaag aacatcaaaa gactcctttg
     1321 agtttgcgat ttttaacaca tattgttgtt gttttttatt ggttgctaga aaattttgat
     1381 ccctttcgct atctaaaggc tccagatcaa caatgtcgct aaaccaagtg ctggctgctt
     1441 gttgtgcatc atctaaactg agccttggtg ctgggctttc aagcgtagga aaagaaaaaa
     1501 tattattaga catttgtttt agctgtgtgg ttcattaagt cgtgtccatg gttattcaaa
     1561 ataatcaaca catcctgctt cttttaaaac agaatcaagt aggtgtcgat ttggaattgt
     1621 ttgatattct tttaataaca ttgtaaatga cttcaggtgg tatttttggg ccgagctaac
     1681 agagtgagaa aaattttctc ctttaatatt aactgacaat tggtcatcac cattgattat
     1741 agcctggttg tttgcagcta accaaggcaa gtatgtggcc cccagctggt cttttaaaat
     1801 aggcatcagt gtttctttta ggtcatccca t
//
LOCUS       DASCTH010000186         2067 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000293313, whole genome shotgun sequence.
ACCESSION   DASCTH010000186 DASCTH010000000
VERSION     DASCTH010000186.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000293313"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2067)
                     /gene="fdhF"
                     /locus_tag="QGH35_03935"
     CDS             complement(<1..>2067)
                     /gene="fdhF"
                     /locus_tag="QGH35_03935"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020400852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HJL81717.1"
                     /translation="CSFKAEIKNNELIRMTPQKDGKANHGHSCIKGRFAWGYTNHQDR
                     IRQPMLRENITDPWQIVSWKRAIEFSASKLLKIQKEFGANSIGGITSSRTTNEEVYVV
                     QKLIRTAFGNNNVDTCARVCHSPTGYGLKQTYGTSAGTQNFDSILESDVIVVIGANPT
                     VAHPVFASQIKKRVRQGARLIVIDPRRTEIVQSPHIKADYHLPLLPGSNVPLMNAFSH
                     IAVKEGLIDDEFINERCDIDSFDSWKSFILSDTHAPKNIEEVTGLDASTITAAARLYA
                     SGGKGSIYYGLGVTEHSQGSTMVMAMANLAMATGNIGKSGCGINPLRGQNNVQGSCDM
                     GSFPHELPGYRAVEDSKVREIFEKKWQVSIQDHPGMRSVNMFDEAVAGTFKGIYIQGE
                     DFAQSEPNIQHIEQAFKSMECVIVQDLFLTETASYAHVFLPATSFLEKDGTFTNAERR
                     INRVRPIMEPITGKHEWEITQELSQAMGYAMNYSSSSEIMDEISELTPSFNGISFDKL
                     DQLGSIQWPCNDEAPEGTPIMHVDRFVRGSGKFMITEFVPTTEVTNRRFPLLLTTGRK
                     LTQYNVGTQTRRTPNNIWGMDDVLEIHPIDASTRGISDNSLVLLQSRKGETTLKVLVT
                     EDIVPGVVYTTFHNPETGANVVTTENSDWATNCPEYKVTAVEISPAEHRSNWQINRSK
                     KLEQLR"
BASE COUNT          563 a          430 c          375 g          699 t
ORIGIN      
        1 gacgtagctg ttcaagtttt ttgcttctgt tgatttgcca gtttgaacga tgttccgctg
       61 gactgatctc caccgcagtt actttatatt caggacaatt ggttgcccag tcactatttt
      121 cggttgtcac gacatttgct cctgtttctg gattatgaaa agttgtatag acaactccag
      181 gtacaatatc ttcagtgacc aataccttca atgttgtttc tccttttctg ctttgaagta
      241 gaactagact attatcactt ataccgcgtg tagatgcatc tatgggatgt atttctagca
      301 catcatccat tccccaaata ttatttggcg ttctcctagt ttgggttcca acattgtatt
      361 gggtgagttt tcttccggtg gttaacaaca atggaaatct tctgtttgtt acctcagttg
      421 ttggtacaaa ctcagtaatc ataaatttac cagagcctct gacaaaacga tccacatgca
      481 tgatgggtgt accttctggt gcttcgtcat tgcatggcca ttgaatgctt cctagttgat
      541 ctaatttatc aaatgagatc ccattaaaac ttggagttag ttctgaaatc tcatccataa
      601 tttctgaaga agatgaataa ttcatagcgt atcccatagc ttgactaagc tcttgagtaa
      661 tctcccattc atgtttaccc gtgataggtt ccatgattgg ccttactcga ttgatgcgac
      721 gttcagcatt ggtgaaagtg ccatcttttt caagaaacga agtagctggt agaaatacat
      781 gggcataact tgctgtttca gtgagaaaaa gatcttgcac aatcacacat tccatggatt
      841 taaatgcttg ttcaatatgt tgaatgtttg gttctgactg ggcaaagtcc tctccttgga
      901 tataaatgcc tttgaaagtg cctgcaacag cctcgtcaaa catattcact gatctcattc
      961 cgggatgatc ttgaatactg acttgccatt ttttttcaaa aatttctctg actttgctat
     1021 cttcgacagc tctgtagcca ggcaattcat gagggaagga tcccatatca caagatccct
     1081 gaacattatt ctgtccacgt aatggattaa ttccacaccc tgatttgccg atattacctg
     1141 ttgccatcgc taaattggcc atggccataa ccatcgtaga accttgcgaa tgttccgtga
     1201 cacccaatcc ataataaatt gaacctttcc ctccagatgc atatagtctt gctgctgccg
     1261 ttatagtaga tgcatctaga cccgttactt cttcaatatt tttaggagca tgggtgtcac
     1321 ttaaaataaa tgacttccaa ctatcaaatg agtctatatc gcatctttca ttaataaact
     1381 cgtcgtcaat cagtccttct ttaacagcaa tatgggagaa agcattcatt aatgggacat
     1441 tggagcctgg taataaaggt aaatgatagt ctgcttttat atggggtgat tgaacaatct
     1501 cagttcgtct agggtcaata actatgagtc tggcaccttg cctaactctt tttttaatct
     1561 gggaggcaaa aactgggtga gccacagtgg gattagcacc aattactaca atgacatcag
     1621 actcaagaat gctatcaaaa ttttgagttc ccgcagatgt tccataggtt tgttttaagc
     1681 cataaccagt aggtgaatga caaactcttg cacatgtatc aacattattg ttaccaaagg
     1741 cagttctgat tagtttttgt acaacatata cttcttcatt agtagttctt gatgaagtaa
     1801 taccaccaat actattggca ccaaattctt tttgaatctt taataatttt gatgctgaaa
     1861 actcaattgc tcttttccaa ctaacaattt gccaaggatc tgttatgttt tctcttagca
     1921 tcggctgtct aatacgatcc tgatgattgg tatagcccca agcaaacctg cctttaatac
     1981 agctgtgccc atgatttgct ttgccatctt tttggggagt cattcttatg agctcattat
     2041 tttttatttc agctttaaat gaacaac
//
LOCUS       DASCTH010000187         8405 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000295092, whole genome shotgun sequence.
ACCESSION   DASCTH010000187 DASCTH010000000
VERSION     DASCTH010000187.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000295092"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="QGH35_03940"
     CDS             <1..749
                     /locus_tag="QGH35_03940"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HJL81718.1"
                     /translation="GFKPRFVSVTYGADGSTQDRTHRAIDILKNELNLHIVPHLTCVG
                     STKEEIQSIANTYWFKGFTSVVALRGDKPDSSLAKDDQSSHYHFALDLTIALKQLHDF
                     TIFTAGYPEVHPEAPSRKFDLENLKQKTEAGAWEVLTQFFYNNDHFYRYRDECQKIGI
                     TARITPGLLPISNFKTISSFTTKCGSEIPKEYSDRFSSLNDQSSMSDLGTELAIKQIE
                     DLRSHGVRKFHLYTLNRKKIMETVIRQAFD"
     gene            complement(763..3375)
                     /gene="aceE"
                     /locus_tag="QGH35_03945"
     CDS             complement(763..3375)
                     /gene="aceE"
                     /locus_tag="QGH35_03945"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HJL81719.1"
                     /translation="MNKQKETAKVVDIDSAQEKRFEKEWKIELDEWLESLDAIKENYG
                     NRQVKELLKSLQNHALSEGMSLGGATLNTPYRNTISLSEQPSYPGNIEMEQRIENILR
                     WNAMAMVLQAYDSGSGVGGHIATYASSATMMEVALNHFIRSRSDEYQGDMFNIQAHAS
                     PGIYARAFLEDRLSIQELENYRRELNPAGGLPSYNHPRSKPKLWPSPGASMGLNGVSS
                     IYYARFAKYLKNRGLTTKKIGKIWAFLGDGEMDEPETIGTINIAAREKLDNLIFVINC
                     NLQRLDGPVRGNGKIIQELEAVFRGAQWNVIKVIWGSEWDPLFAIDHNDVMQKRMDEV
                     VDGEYQKYSVSSGAEQRAHWINGNLELETIMTNLSDTELKDIKRGGFDHKKLYAAFDK
                     AINSEGKPTVILIKTLKGYGLGEGSEGRNIAHQKKQMSDEERLQNAARLNIPLSEPEI
                     KDAKFFVPDPKSDEVKYLKERRVALGGYQPIRFNECKKIKTPDIELFQDFLDGVDRSI
                     STTMALVRMMAKLLRDKEVGEYIVPIVPDEARTFGMDGLFPQAGIYSPEGQHYHPVDE
                     GTIAPYKEAKDGQILQEGICEAGAMASFMAAGTAYSHFELPMIPFYIFYSIFGFQRVG
                     DMLWACGDALTRGFLIGGTAGRTTLNGEGVQHQDGHSHVLASVIPNLMTYDPAFAYEL
                     AVIIREGIRRMYEEEENIFYYITVYNENYTHPKLPKGAEEGILKGIYLLNKSSEKLMK
                     KYKGEPIHLLGSGSIMQQVLNAQKELEKKGIPVNVWSVTSYNLLHKDFLESKKSNQEP
                     YLAKIMKNETGHFIAVSDFMTLLPDSLSHLFPGTFTSLGTDGYGLSEAREELRDYFGI
                     SAKSIIDCVLQRLN"
     gene            3485..4807
                     /gene="yegQ"
                     /locus_tag="QGH35_03950"
     CDS             3485..4807
                     /gene="yegQ"
                     /locus_tag="QGH35_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HJL81720.1"
                     /translation="MTTSELLSPAGSLKGMHFAFAYGADAVYAGQPKYSLRVRNNEFR
                     KQDNLAAGIAEAHRLGKKFYVASNLAPHNNKVRTFIKDMASVVEAKPDAVILSDPGLI
                     MMVRERWPELTVHLSVQANAVNFATVKFWQRQGVHRVILSRELSLDEIEEIRQQCPDI
                     ELEVFVHGALCMAYSGRCLLSGYMTHRDSNQGACTNSCRWNYQAHPAVEDSSGDLVAT
                     PDSLTIDTPAMLLQEEKRPGEYMPAFEDEHGTYIMNSKDLRAVQHVGRLIELGIESLK
                     IEGRTKSHYYVARTAQVYRRAIDDALAGRTFDMSMMDQLDSLANRGYTEGFYRRHPPH
                     EFQNYEHGFSRGLKQQYVGDVNKDDTHPGMLAIDVKNRFQVGDELELMTPNGNLSLHL
                     THLEDEQGQSIKLAPGSGYKVFMPQPTDFQSTSDAEFSMVLRNLETQE"
     gene            complement(4832..5203)
                     /locus_tag="QGH35_03955"
     CDS             complement(4832..5203)
                     /locus_tag="QGH35_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81721.1"
                     /translation="MVARILSGLVGVLMLLNFFWWLLDPATAAEELAMSLQEGLGGNT
                     QIGDFTSFFFTAGLFACIGAYRAEYIWLYTTISLLASAAIFRSYAVVVHESEPLMSAI
                     GSELVMSVALIACVFLMKRTQ"
     gene            5291..7144
                     /gene="ilvD"
                     /locus_tag="QGH35_03960"
     CDS             5291..7144
                     /gene="ilvD"
                     /locus_tag="QGH35_03960"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HJL81722.1"
                     /translation="MPEYRSKTSTHGRNMMGARALWRATGMKENDFGKPIIAIANSFT
                     QFVPGHVHLKDLGQMVAKEIYTNGGIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SVEYMVNAHCADALVCLSNCDKITPGMLMASMRLNIPTIFVSGGPMEAGNAKVRGLDT
                     KLDLIDAIVFSGDDSTSEEDITAIERSACPTCGSCSGMFTANSMNCLVEAMGLALPGN
                     GSILATHVDRQQLFLDAGKLIVEITKEFYEKDNDGVLPRNIVNNKSLENAMTLDIAMG
                     GSTNTVLHILATAKEAHVDFSMDDIDRLSRRVPNLCKVAPSSNYHLEDVHRSGGVMAI
                     LGELEKGKLLNIDTTTVHSKTLKSAIEKWDINRNPDKEIIQFYSAAPGMVPTIEPFSQ
                     KKRYESLDLDREQGCIRQIDHAYSRDGGLAILKGNIALDGCIVKTAGVDENNLTFSGP
                     AHIFESQDDAVDGIQNDLVQPGEVVVIRYEGPKGGPGMQEMLHPTSYLKAKGLGKKCA
                     LITDGRFSGGTSGLSIGHISPEAASGGAIGLIRDQDIIEIDIPNRTINVQLSAEEIEN
                     RRIAMTDIGTRAWKPKNRRREISQALQAYALMASSASEGAVRVLPSEHDDQ"
     gene            7134..7790
                     /gene="npdG"
                     /locus_tag="QGH35_03965"
     CDS             7134..7790
                     /gene="npdG"
                     /locus_tag="QGH35_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008490320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HJL81723.1"
                     /translation="MTNRKMKIAVFGGTGALGSALSIKWAAKGYPVIIGSRSREKAES
                     IVCSIKEEYDLQSIEGSGLEDAAQQCDLAVLTVPYVSHETTLKIIREYIQGKVLIDST
                     VPLQKDSSRVLLPKKGSAAMEAQDILGPDVKVISALQNIGSHLISSNKPVDAEVLVSG
                     NDEEAVNTVTQLIEDLDLRSWHAGPLENSAAAEALTSVLIAINKKYKLKSSSIKITSN
                     "
     gene            7880..>8405
                     /locus_tag="QGH35_03970"
     CDS             7880..>8405
                     /locus_tag="QGH35_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HJL81724.1"
                     /translation="MIEKKDDLSNLVLTCLDRNEISIETNDIIAIAQKIVSKAEGRLM
                     KLSDVKPTQKAVRLARQANKDPRLVELILQESNEILRVSDGVIIVEHRLGHVLANAGI
                     DRSNIKSTHQEEMVLLLPINPDQSAENIRNEIKKKIGINVGVIITDSIGRAWRLGTIG
                     HAIGSAGIKTIMDLR"
BASE COUNT         2627 a         1692 c         1682 g         2404 t
ORIGIN      
        1 ctggattcaa acctagattt gtgagcgtaa cctatggtgc tgatggctcc actcaggata
       61 gaacacacag ggctattgac atcttaaaga atgaactgaa tcttcatatt gttcctcatt
      121 taacttgtgt gggatccacc aaagaagaaa tccaatcgat tgcaaataca tattggttca
      181 aaggcttcac atcagttgta gctttaaggg gtgacaagcc ggatagctct cttgcaaagg
      241 atgatcaatc cagtcattat cattttgctc ttgatctcac tattgcctta aaacaactac
      301 acgatttcac tatttttaca gcgggttacc ctgaagttca tcctgaagct ccaagtagaa
      361 aatttgatct ggaaaattta aaacagaaaa cagaagctgg ggcttgggaa gttctcactc
      421 aattctttta taacaatgat catttctata gatacagaga tgagtgccag aaaattggta
      481 ttacggcacg aatcactcct gggctattac caattagtaa ttttaaaact atctcctctt
      541 ttacaacaaa atgtgggtca gaaatcccta aagaatactc agatcgattt agctcattga
      601 atgatcaatc aagtatgtcc gatctaggta ctgagttggc aattaaacaa attgaggatt
      661 tacgttctca tggagttaga aagttccatc tgtatacatt gaacagaaag aagataatgg
      721 agacagttat tcgtcaagca ttcgattaaa agtaaaaata aattaattta agcgttgcaa
      781 gacacaatca atgatggatt ttgcactgat tccaaaataa tctcgtaatt cctctctggc
      841 ttcactcaat ccataaccat cggttcccag agaggtaaaa gttcctggaa aaaggtggga
      901 caagctatct ggcaaaaggg tcataaaatc actcacagct ataaaatgtc ccgtctcatt
      961 tttcattatt ttggcaagat aaggttcttg gttggatttc ttggattcaa gaaaatcttt
     1021 atgtaacaga ttatagctgg taacactcca cacattcaca ggtatacctt ttttttctag
     1081 ttctttttgt gcatttaata cttgttgcat gatgcttcca cttcccaata aatgtatggg
     1141 ttctcctttg tactttttca ttaatttttc agagctttta ttcaagaggt aaatgccttt
     1201 taggattcct tcttcagctc ccttagggag tttaggatga gtgtaattct cattgtaaac
     1261 cgttatataa tagaaaatat tttcttcttc ctcatacatt cgacgtatcc cttccctgat
     1321 aataactgcc aattcataag caaatgcagg atcgtaagtc attaaattag gaatgaccga
     1381 agccaacaca tgcgagtgac catcttgatg ttggacacct tcaccattaa gagtagttct
     1441 gccagctgta cctcctatta aaaagccccg ggtcaaggca tccccgcaag cccaaagcat
     1501 atctccaacg cgttgaaaac caaaaattga atagaaaata tagaacggta tcattggcag
     1561 ttcgaagtgg gaataagccg ttcctgcagc cataaaagag gccatagcac cggcttcaca
     1621 aattccctct tgcagtattt gcccatcttt ggcttccttg tatggagcaa tcgtaccttc
     1681 atcgacggga tggtaatgct gcccttccgg tgagtagatg cctgcttgag gaaagagtcc
     1741 atccatacca aaggtccgag cttcatcggg aactattgga acaatatatt caccaacctc
     1801 cttgtctcta agaagttttg ccatcattct taccaaagcc attgtagtag aaatagaacg
     1861 atccactcca tcaagaaaat cttggaatag ttcaatgtca ggagttttaa tttttttgca
     1921 ctcattaaac ctgataggct gatacccacc gagtgctact cgtctctctt ttaaatattt
     1981 aacctcatct gatttaggat caggaacaaa aaactttgcg tctttgatct ctggctctga
     2041 aagagggata ttcaatcttg ctgcattctg taacctctcc tcatcactca tttgcttttt
     2101 ctggtgagca atattccttc cttcagagcc ttctcctaac ccataacctt taagggtttt
     2161 aatcagtata actgtaggtt tcccctctga attaatggct ttatcgaagg cagcgtagag
     2221 ttttttatga tcaaaaccac cacgtttaat atctttaagc tcagtatcac taagatttgt
     2281 catgatggtt tctaattcta gattcccatt gatccagtgt gcacgttgct cagcacccga
     2341 tgagacagag tacttttgat actcaccatc aactacttca tccattcttt tttgcatgac
     2401 atcattatga tcaatggcaa aaagaggatc ccattctgac ccccagataa cttttatcac
     2461 attccactga gcgcctctaa aaactgcttc cagttcttga ataatttttc catttcctct
     2521 gaccggacca tcaagacgtt gtaaattaca attgataaca aaaataagat tgtccagttt
     2581 ttccctcgca gcaatattga tggttcctat agtctcgggt tcatccattt ctccatctcc
     2641 aagaaatgcc cagatttttc ctattttctt agtggtcagc cctcgatttt ttaaatattt
     2701 agcaaaacgg gcgtaataga ttgaagacac accattcaaa cccatggaag caccaggact
     2761 tggccacagt tttggtttac tcctaggatg gttgtaagaa ggcaaacccc ctgcagggtt
     2821 taactccctt ctatagtttt ctaactcttg gatactcaag cgatcttcca aaaacgctct
     2881 ggcataaata cctggggatg cgtgagcttg aatattaaac atatctcctt gatattcatc
     2941 agatcttgat cgaataaaat gattcaatgc aacttccatc attgttgctg aagatgcata
     3001 agtagcaata tgtccaccta ctcccgaacc gctatcgtat gcctgtaaaa ccatggccat
     3061 ggcattccat cggagaatat tttcgattct ttgctccatt tcaatattcc ctggatagct
     3121 cggttgttca gagagtgaaa tggtattgcg atatggagta ttgagtgttg ctccacccag
     3181 cgacataccc tcacttaacg catgattttg aagagatttt aatagttcct taacttgtcg
     3241 gttgccatag ttttccttga tggcatcaag tgattccagc cattcatcta gctcaatttt
     3301 ccattctttc tcaaaacgtt tttcctgagc cgaatcgata tctaccactt tggcagtctc
     3361 tttctgttta ttcatcagta aatcttggac aataattgtt attattataa ttcttaggat
     3421 atacgatacg atatgaatag tctttcaaga ttccaaagca agccttacta agatactcaa
     3481 gattatgaca acatctgaac ttttatcacc agctggatca ctcaagggaa tgcattttgc
     3541 attcgcctat ggtgctgatg cagtctatgc tggccagcca aagtacagtt tacgggtcag
     3601 aaacaacgag tttagaaaac aggataattt ggcggctggt atcgcagagg ctcacagact
     3661 gggtaaaaaa ttctatgtag ctagtaatct ggcacctcac aacaacaagg tacgtacttt
     3721 catcaaagat atggcttctg tagtagaagc aaaacccgat gctgtgattc tttctgatcc
     3781 gggtctgata atgatggtta gggaacgttg gccagaatta acagtgcatc tttctgtaca
     3841 agctaatgca gtcaattttg caacggtaaa attctggcag agacagggag ttcaccgagt
     3901 tatcctttca agagaacttt cactcgatga aatcgaggaa attaggcaac agtgtcccga
     3961 tattgaactg gaagtatttg tacacggtgc tctgtgcatg gcctactcag gccgctgttt
     4021 actttcaggc tatatgactc atcgggattc caatcagggt gcctgtacaa attcatgccg
     4081 gtggaattac caggcacatc cggctgtcga ggatagtagt ggtgatctgg tcgccacgcc
     4141 agattcctta acaatcgata ctccggcgat gttattgcag gaagaaaaac gtccaggaga
     4201 atacatgcct gcctttgaag atgagcatgg tacctatatc atgaattcca aggatttacg
     4261 tgcagtccag catgtgggac gtctcatcga actaggtatt gaatctctaa aaatagaggg
     4321 tcgcactaaa tcacattatt acgtagcgag aactgctcag gtgtaccgac gcgccattga
     4381 cgatgccttg gcgggcagaa cattcgatat gtcaatgatg gaccagcttg attcactggc
     4441 caatcgagga tacacagagg gattttatcg tcggcatccc cctcatgaat ttcagaatta
     4501 tgagcatggc ttttcaagag gactgaaaca acaatatgtt ggtgatgtga ataaagatga
     4561 tactcacccc ggcatgttgg caatcgatgt aaaaaatagg ttccaggttg gagatgaact
     4621 agagttgatg acaccaaacg gaaatctttc tctgcatctc acgcatcttg aggatgagca
     4681 aggtcaatcg attaaacttg cacccggttc tgggtacaag gtattcatgc cccaaccgac
     4741 tgatttccag tctacctctg acgctgaatt cagtatggtg ttgagaaatc tggaaacgca
     4801 agaatagtac agcctgtgta aaagcatgtt ttcactgagt tcttttcatt aagaatacac
     4861 aagctatcaa agctactgac atgacaagtt cagaacctat ggcagacatt aaaggttctg
     4921 attcatgaac aaccactgca taagacctaa agattgcagc agaagcaagt aaagatattg
     4981 tggtataaag ccaaatgtat tccgctctat aggctccgat gcaggcaaat agccctgcag
     5041 taaaaaagaa agaagtaaaa tctccaattt gagtgttccc tcctaaacct tcctgtaagc
     5101 tcatcgctaa ttcttcagca gcagtagctg gatccaacaa ccaccaaaaa aaattcaaca
     5161 gcattaacac tcctaccaag cctgataata ttcttgcaac catattttac tcctaaggta
     5221 atatgagaat tagaattata aataataaat gagataataa tacccaaata aaacgaccct
     5281 aaaaaaacat atgcctgaat atagatcaaa aacctcaact catggaagaa acatgatggg
     5341 agcaagagcg ctatggcgtg caacaggcat gaaagagaat gattttggca aacccattat
     5401 tgctattgca aattcattta ctcagtttgt gcctggccat gtccatctga aagacttagg
     5461 acaaatggtg gcaaaagaaa tctataccaa tggcgggatt gctaaagaat tcaataccat
     5521 tgccattgat gatggaatcg ctatgggtca ttcaggaatg ttgtatagtt tgccgtcacg
     5581 agatttaatc gcggattcag ttgaatacat ggtcaatgcc cactgtgcag acgcactggt
     5641 ttgtttgtcc aattgtgaca agattacacc cggtatgttg atggcaagca tgagattgaa
     5701 tattccaacc atttttgtct ctggaggccc tatggaagca ggcaatgcca aagtcagagg
     5761 cttggataca aaattggatt tgattgatgc aatagttttt tcaggagacg attctacttc
     5821 agaagaagat attacagcca ttgaaagatc ggcatgcccg acttgtgggt cttgttcggg
     5881 tatgtttact gccaattcaa tgaattgtct agtagaagca atgggacttg cactgccagg
     5941 taatggcagc attctggcaa cgcatgttga tcgtcaacaa ctatttctag atgcaggtaa
     6001 attaattgtt gagataacaa aagaatttta tgaaaaagac aatgacgggg ttttaccacg
     6061 caatatcgtt aacaacaaat cactcgaaaa tgcgatgaca ttagatatag caatgggagg
     6121 atcaacgaat acagttttgc acattcttgc aactgccaag gaagcccatg ttgatttttc
     6181 aatggacgac atagatcgtt tatctagacg tgttccaaat ctttgcaagg tagcaccaag
     6241 tagcaattac cacttagaag atgtacatcg ttcaggaggt gttatggcta ttcttggcga
     6301 actcgaaaaa ggtaagttgt taaatataga cacaacaaca gtccatagca aaacattgaa
     6361 atcagcgatt gaaaagtggg acatcaatag aaatcctgat aaagaaataa tccagtttta
     6421 ttctgctgcc cctggaatgg ttcctaccat tgagcctttt tcacagaaaa aacgttacga
     6481 atcactagat ttagatcggg agcagggttg cattcgtcaa attgaccatg cgtatagcag
     6541 ggatggagga ttggccattc tcaaaggtaa tattgccttg gatgggtgta tcgtgaaaac
     6601 tgctggagtt gatgaaaata atttaacctt tagtggccca gcacatattt ttgaaagtca
     6661 agatgatgct gttgatggaa ttcaaaatga tctagttcag ccaggagaag tagtggtcat
     6721 tcgctacgaa ggccctaaag gaggtccagg aatgcaagaa atgttacacc ctaccagtta
     6781 tttaaaagca aaaggtttgg gtaaaaaatg tgctctgatt acagacggac gattctcagg
     6841 aggaacctct gggctttcta tcggacatat ttcaccggaa gcagcttctg ggggagcaat
     6901 aggattaatc agagatcaag acataataga aatagatatt cctaatcgaa caattaatgt
     6961 gcaactttct gcagaagaaa tagaaaacag gagaatagca atgactgata tcggcaccag
     7021 agcttggaag ccaaaaaatc gaagacgtga aattagtcag gccttgcaag cttatgcgtt
     7081 gatggcttca tctgcttcag aaggagcggt tagagtttta cccagtgagc atgatgacca
     7141 atagaaaaat gaaaatcgct gtttttggtg gtacaggagc tttgggttca gcactctcga
     7201 tcaaatgggc agccaaaggt tacccagtca tcataggatc gagatcaaga gaaaaagctg
     7261 agagtattgt ctgttctata aaagaagaat acgatttgca atccatagaa ggatctggat
     7321 tggaagatgc agctcaacaa tgcgatctag ccgtcttgac ggtcccgtac gtaagtcacg
     7381 aaactacttt gaaaataatc agagaataca tacaagggaa agtgctaatc gattcaacag
     7441 tgccacttca aaaagatagt tccagagttt tattgccaaa aaaaggctct gctgccatgg
     7501 aagcacaaga tattttaggt ccagatgtta aagtgatatc ggcacttcag aatattggat
     7561 ctcacctgat aagctccaat aaacctgtcg atgctgaagt gctggtctca ggaaatgatg
     7621 aggaagcagt taatactgta acccagctta tcgaagatct agatttgcga tcctggcacg
     7681 caggtccact agaaaactca gcagcagcag aagccctaac ttctgtattg atagcaatca
     7741 ataagaaata caaactaaaa tcttcaagta ttaaaattac atctaactga cgaaaggcct
     7801 tattttaaaa ctcttgagta acatgtattt taaaacaact ccaccttcaa tctcattatt
     7861 tgcccctaca ggtttcccga tgattgaaaa gaaagacgat ctttcgaatc tagtcttgac
     7921 ttgcttggat aggaacgaaa taagtattga aacaaacgat atcattgcta ttgcccaaaa
     7981 aattgtatcg aaagccgaag gacgtttgat gaaattatct gatgtcaaac caacccaaaa
     8041 agcagtacgt ttagccaggc aagccaacaa agatccaaga ttggttgaat taatccttca
     8101 ggaatcaaat gagatcttaa gggtcagtga tggtgtaata attgttgagc atcgtttagg
     8161 acatgtttta gccaatgcag gaattgatcg gtcaaatata aaatcaacac accaggaaga
     8221 aatggtattg ctgctcccca tcaatcctga tcaatctgct gaaaatatta gaaacgaaat
     8281 taagaagaaa attggtatca atgtaggtgt aattatcacc gacagtatcg gcagagcttg
     8341 gcgattgggg actattggtc atgcaattgg ttctgctggg attaaaacaa taatggatct
     8401 tcgtt
//
LOCUS       DASCTH010000188         2025 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000302289, whole genome shotgun sequence.
ACCESSION   DASCTH010000188 DASCTH010000000
VERSION     DASCTH010000188.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000302289"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="QGH35_03975"
     CDS             <1..239
                     /locus_tag="QGH35_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HJL81725.1"
                     /translation="ALYLIDEVLTPDSSRFWAMEDYHLGISPPSFDKQYLRDYLETLD
                     WEKTEPGPELPSEIIENTAAKYQSAYDIITERIN"
     gene            complement(295..564)
                     /locus_tag="QGH35_03980"
     CDS             complement(295..564)
                     /locus_tag="QGH35_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81726.1"
                     /translation="MKREPEKKENPYLIIGFWEGLAYTTVFVILFPWSLLYLLIFRGA
                     ENTKDFLVFFVRDLLITIVITVISIILIIAPAIYYLVSWIAQWFS"
     gene            complement(649..2019)
                     /locus_tag="QGH35_03985"
     CDS             complement(649..2019)
                     /locus_tag="QGH35_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJL81727.1"
                     /translation="MSFKKLGLSDELLNAIQAKGYSQTTPVQEKAIPYIMQGLDILAG
                     AQTGTGKTAAFALPILSRLQQSESKRRRIRALVLTPTRELASQVADSFRDYGSNLRFK
                     TAVIYGGVSIKTQKDKLRNGVDIVVATPGRLLDHLNQKTLDLSEIEVFVLDEGDRMLD
                     MGFIVDIKKIIKHLPQKRQNLLFSATFPKEIKLLAARLLNLPKQIQMSSQNSTAENVK
                     QKIYPVDQARKRELLIHCIKKENWFQVLVFIRTKRAADKLAKQLNKQRIKANAIHGNK
                     TQGARTRALKSFKDGEIQVLVATDVAARGIDINLLPIVINYNLPIVPEDYIHRIGRTA
                     RAGKEGTAISLVCADEFNLLSEIERLLKFRIPREDIEGFETTQHLNTTNLESKNNQRK
                     KSYRGNQQLRIKLSDNKKPPWKRKRKSPKREEKNKNQSKQLSSKKKKPFKKSKFKKRI
                     TKSQLS"
BASE COUNT          568 a          368 c          335 g          754 t
ORIGIN      
        1 gagcgttata tctaattgac gaagtgctga ctccagattc atcaagattc tgggcaatgg
       61 aagattatca tcttggtatc agtccaccca gttttgacaa gcagtacctg agagactatt
      121 tagaaacatt ggattgggag aagactgaac ctggacctga gctaccgtca gaaatcattg
      181 aaaacactgc ggctaagtat caaagtgcct atgacatcat cacagaacgg attaactgat
      241 tcgaaaggat agtgtcttcc ttaaacagtt tatttgacta attttttagc ttcctcaact
      301 gaaccactgt gcaatccaag aaactaaata ataaatcgct ggcgctataa ttaagataat
      361 tgaaatgacg gtgatcacta ttgtgatcaa gagatctctc acaaaaaaaa ctaaaaagtc
      421 tttggtgttt tcagcacctc gaaaaattaa aagatatagt aaggaccatg ggaataaaat
      481 aacaaatact gttgtgtatg ccagaccttc ccagaatcct attattaaat atggattttc
      541 ttttttttcc ggttctcttt tcaaattcta tgcctaatac cctataccca aagcctacaa
      601 caaatctgtc aaatatcaca aatgttgatg ctggtggatg gtttggtttt aagacagctg
      661 gcttttagtt atcctttttt taaactttga ttttttaaat ggtttcttct ttttactgct
      721 taattgctta ctttgatttt tgtttttctc ttctctttta ggagattttc tttttctttt
      781 ccaaggaggt tttttattat cagacagttt tattcttagc tgttggtttc cacgatagga
      841 tttctttctt tgattatttt ttgattctaa atttgttgta ttaagatgct gtgttgtctc
      901 aaagccttca atatcttctc ttggtattct aaattttaat aaacgttcaa tttcgctcaa
      961 taggttaaat tcatccgcac agactagaga gatagctgta ccttcttttc ctgctcttgc
     1021 agttcttcca attcgatgaa tataatcctc aggaactatt ggcaagttgt agttaataac
     1081 aattggaagt aagttaatat ctattcctct agcagcaaca tcagtggcaa caagtacttg
     1141 aatctctcca tctttaaaac tttttaaggc tctagtccta gctccttgtg ttttattacc
     1201 atgtatagca ttagctttga ttctttgttt attcagttgc ttggctaact tatctgcagc
     1261 tctttttgtt cttatgaaaa caagtacttg aaaccagttt tcttttttta tgcaatggat
     1321 gagcaattct ctttttcttg cttggtcaac aggatagatt ttttgtttca cattttcagc
     1381 agtagagttt tgtgaagaca tttgaatttg tttgggcaag ttgagaagtc ttgcagccaa
     1441 taatttaatt tctttaggga aagtggcaga aaatagtaga ttttgtcttt tttgaggaag
     1501 gtgtttaata atctttttta tatccactat aaaacccata tcaagcatcc tgtcaccttc
     1561 gtctaaaaca aaaacttcta tttcagacaa atccaatgtt ttttgattta agtgatcaag
     1621 caacctacct ggtgtagcaa caactatgtc aacaccattc cttagcttat ctttctgtgt
     1681 tttgatgctt actccaccat atattactgc tgttttgaat cgtaagttac taccatagtc
     1741 acgaaaacta tcagcaactt gagatgctaa ctctcttgtt ggtgttaata ccaatgcacg
     1801 tattcttctt ctttttgact cagattgttg cagtctactc aatataggca aagcaaacgc
     1861 tgctgtcttg ccagttccag tttgcgctcc tgcaagaata tctagacctt gcattatgta
     1921 tggtatagct ttttcttgga ctggtgttgt ttgtgagtag ccttttgctt gaattgcatt
     1981 caataactca tctgaaagtc ccaacttttt gaaagacatt actta
//
LOCUS       DASCTH010000189         5992 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000303078, whole genome shotgun sequence.
ACCESSION   DASCTH010000189 DASCTH010000000
VERSION     DASCTH010000189.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000303078"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            23..535
                     /locus_tag="QGH35_03990"
     CDS             23..535
                     /locus_tag="QGH35_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024834.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HJL81728.1"
                     /translation="MSDSGLGFSYKKFIALIIVFACCILLFEFVGNFGGNLIKAQVKD
                     TTSKYNKDRTQEDRISPVFTLAKKQQQTSEITTVKTVPTSLTTAMNGESVYNSACLAC
                     HTTGAGGAPLLGDSTAWETRMEQGIGTLTKRAVEGYVGNSGIMPAKGGRLDLTDQEVE
                     SAVVYMLEQF"
     gene            541..954
                     /locus_tag="QGH35_03995"
     CDS             541..954
                     /locus_tag="QGH35_03995"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HJL81729.1"
                     /translation="MVTSVVFYSGDLRAESEHLQSGNKIITDAPIDNEGKGEGFSPTD
                     LVATSLANCMLTIMGIVARRHEVNIDGTRADVEKIMGTEPRRIAEIQIEFFFPNSYDS
                     KTKIILEQAALNCPVAKSLAESLHQSISFNYDGSS"
     gene            complement(968..1201)
                     /locus_tag="QGH35_04000"
     CDS             complement(968..1201)
                     /locus_tag="QGH35_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HJL81730.1"
                     /translation="MENNNFEDWTKKISALLPDSALQAKEDIEKNIRFLIKDAIKKMD
                     LIERTELEEFCSIQEKAIQELQQRINELEVGIK"
     gene            1282..2379
                     /locus_tag="QGH35_04005"
     CDS             1282..2379
                     /locus_tag="QGH35_04005"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HJL81731.1"
                     /translation="MTTNQSSQKGDLPTKAPEKKTGVVSKFAWFVILVLLISGIGTSF
                     WYGQRLILSFDESLNQTTKQFEATASALSENNQVLDSIMTDQENMAGFFKAQATILEQ
                     LQNKSMTQQELLTDLQNQVALIPSVPADDRNNWLISQAKYFLTMAQAELLLFGNQASA
                     QSALRSALQSIQLLPDRYLAINQAIKLALSKLDPEGSTIKKNQIIGRLQNIDRSFVLL
                     LSNKINMNQIEEQSTTKKTFDLMLRAAGTKITKAIEELIIVRTDGDTRSLKLSKTQEQ
                     IMKSMVSLNLNMARIAILQSNDDQFQSSLDGCEELIKQTLGINAAGITEQPPGVIELL
                     NELKQLKDFRLKPSISEIQDAITLLEAVDSL"
     gene            2376..3536
                     /locus_tag="QGH35_04010"
     CDS             2376..3536
                     /locus_tag="QGH35_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HJL81732.1"
                     /translation="MKFALWGFLLLVLIGLGVHFLLEDNGYVLIEFLSYSFETSLPIF
                     IIVLIVLYLLVRLLGLIWHSPKILKQKMAAKKFEKSNKLLHEGLNLVGKGNFTKAEKK
                     LKASIDDIHASLLAFLMVSDISQKNGGEQSRDAWLEKALAKYPESEIYILLHASQQRI
                     KEQDYTAAQDYINTILDKDPNNPIALKLLSQCLYKTQQWRLLTQKAKSIIEQVKPNQM
                     TDLYIAKAFTESAKELVKDQQQMETLWHKVPYSLTNNVLILTTKIESMMLNGQVNAAE
                     KELRKHIPKTWDKKLIALFAKLESPDLSKLSKQMDRWLVDRPRDANLWQAASHLAKRD
                     ELWKQAKQFIERSIEINENPFAYNQLGFILLELGQQDAAIKAFEQAMNLNND"
     gene            3606..4505
                     /gene="xerC"
                     /locus_tag="QGH35_04015"
     CDS             3606..4505
                     /gene="xerC"
                     /locus_tag="QGH35_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020159057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HJL81733.1"
                     /translation="MNKNAEQLKKNFLHHLETEKRFSVNTITSYQRDLASLESYCFDN
                     QINSWQELKPHHLRAFSSKIFNRGLGPRSIQRRLSGIRSFMNYLLRENLIKSNPANGI
                     RTPKAPKRLPDVLDVDQVNQLLNIKTTNPISLRDKAILELLYSSGLRLSELVSLNPID
                     LNLKDKSMTVQGKGEKTRLLPIGQKAIEAVNQWLEVRVQLAHMDEEALFVSLKGRRLS
                     QRAVQSRIDHWAKQTGMQQNIYPHLLRHSFATHLLEASGDLRAIQELLGHKDISTTQI
                     YTHLDFKHLAETYDKTHPRSGKK"
     gene            4502..5254
                     /gene="ubiE"
                     /locus_tag="QGH35_04020"
     CDS             4502..5254
                     /gene="ubiE"
                     /locus_tag="QGH35_04020"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008488203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HJL81734.1"
                     /translation="MKQDLNKNFDFGYERVTEEEKTDKVGAVFDSVSQKYDLMNDLMS
                     LGLHRFWKRFAMMHTGLIEGMSALDVAGGTGDLASSLCQQVGKKGTVVLTDINFNMLI
                     NGRSKLLDQGKLNQINLIQSNAESLPFVNDSFDCITIGFGLRNITNKEKALASIMQVI
                     KPGGRLLILEFSKPNELISPFYDFYSFSVLPKLGDWMVNDADSYQYLAESIRMHPDQK
                     KLKEMMETIGFSNCEYFNLTGGIVALHIGYKI"
     gene            5273..>5992
                     /locus_tag="QGH35_04025"
     CDS             5273..>5992
                     /locus_tag="QGH35_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015094.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HJL81735.1"
                     /translation="MQQFKILARLLHIHKILVDYRLDDLIRDIPYLKPLQWLFYFSPK
                     RWLSNKNQLPRAIRIRRALEKLGPLYVKFGQSLSTRPDLLPEDIANELTQLQDNVPAF
                     SSEAAEQEIETAFGRSAQEVFKSFNSDAFASASVAQAHLAELQSGEEVVVKILRPGIL
                     DAIEKDMQILFILAKLIEGYSKEGKRLRPLEVVSDYQKVVHNELDLMREAANCAQIGR
                     NWKDSDIIHVPKVYWDYCRPNV"
BASE COUNT         2024 a         1096 c         1148 g         1724 t
ORIGIN      
        1 aaacagaaaa taaggagcta tcgtgagtga ttcgggatta ggtttctctt ataaaaaatt
       61 tatagcttta atcattgttt ttgcttgctg tatattgttg tttgagtttg taggcaattt
      121 cggcggtaat ttgatcaaag ctcaagtcaa agacaccacg agtaaataca acaaagacag
      181 aacgcaagaa gatagaattt cacctgtctt cacgctagca aaaaaacaac aacaaacatc
      241 tgagatcaca actgtgaaaa ctgtccctac ttcattaacg actgcaatga atggtgaatc
      301 agtctataac tctgcttgcc ttgcttgtca tacaaccgga gcgggaggcg cacccctttt
      361 gggcgattca acggcttggg aaaccagaat ggaacaaggt attgggactc tcaccaagcg
      421 cgccgttgaa ggctatgtag gcaatagtgg cattatgccc gcaaaaggtg gtcgtcttga
      481 tcttactgac caagaagtag aaagtgctgt tgtttatatg cttgagcaat tttaaagttg
      541 atggtcacat ctgtagtttt ttattctggc gatttgagag ctgagagcga gcaccttcaa
      601 tctggaaata aaattattac cgacgcccca atcgataacg aaggaaaggg tgagggcttt
      661 tcaccaactg acttggttgc cacttccctt gcaaattgta tgttaaccat aatggggatt
      721 gttgcccgtc gacacgaggt taatattgat ggaactcgtg ctgatgtaga aaaaataatg
      781 ggcactgaac caagaagaat tgctgaaata caaatcgaat tctttttccc caatagttat
      841 gattcaaaaa caaaaataat actggagcaa gcggcactaa actgccctgt tgctaaaagc
      901 cttgctgaat cactacatca aagtattagt tttaactacg atggcagctc atagaaagat
      961 ttttctatta ttttatccct acctctaatt cattgatgcg ctgttggagc tcttgtattg
     1021 ctttttcctg aatcgaacaa aattcttcta attcagttcg ctctatcagg tccatctttt
     1081 ttatcgcatc tttgatcaga aatctaatat tcttctcaat atcttcctta gcctgcagtg
     1141 cagagtcggg caacaatgca gatatttttt tcgtccaatc ttcaaaatta ttattttcca
     1201 tcatcgattc cttgtaaaaa cttaagtatt atcattaatc ataagcagaa caactgaatt
     1261 aactagtata gctttaaaaa aatgactact aatcaatcat cccaaaaagg cgatcttccg
     1321 actaaggcgc cagaaaaaaa aacaggagtt gtatctaaat ttgcatggtt tgtaatactg
     1381 gtattattaa tctctggaat cgggacttcg ttctggtatg gacaacggtt aatactctcg
     1441 tttgatgaat ctttaaatca aacaactaaa cagtttgaag caactgctag tgcgctctct
     1501 gaaaataatc aagtgctgga ttctattatg actgatcaag aaaatatggc aggctttttt
     1561 aaagcacaag ctactattct agagcaatta cagaacaaaa gtatgacaca gcaagagcta
     1621 ttaactgatc tacaaaatca agtggctttg ataccttcag tgcctgccga tgatcgaaac
     1681 aattggttga tcagccaagc taaatatttt ttaactatgg cacaagcaga actcttatta
     1741 tttgggaacc aagcatcagc acagtctgcc ttacgctctg cactacaatc tattcagctt
     1801 ttacctgacc gttatttagc aatcaatcag gcaataaaat tagctttatc aaaacttgat
     1861 cctgaagggt caacaataaa gaaaaatcaa attataggaa gattgcaaaa tattgataga
     1921 tcctttgttt tgcttttaag caataaaatt aatatgaatc aaatagaaga acagtcaacc
     1981 actaaaaaaa catttgatct aatgttgagg gcagctggta caaaaataac taaagctatc
     2041 gaagaactga tcattgtaag aacagatggt gataccagaa gcttaaaact cagcaaaacc
     2101 caagaacaaa taatgaaatc aatggttagc cttaatctca acatggcaag gattgcaatt
     2161 ttgcagagta atgatgatca atttcaatcc tcactggacg gttgtgaaga gttgattaag
     2221 caaacactcg gtataaacgc agcgggcatc actgaacaac cacctggtgt catcgaactg
     2281 ctcaatgagc ttaaacaatt aaaagatttt agattaaagc caagtatttc agaaattcaa
     2341 gatgcaataa ctttgttaga agcggtagac tcattatgaa atttgctctg tggggattcc
     2401 tgttactggt tctgatcggc ctgggagtcc attttttatt agaagataat ggctatgttc
     2461 tgattgaatt tctcagttat tcctttgaaa catcgctgcc aatttttatc atagtattaa
     2521 tagttctcta tctcttagtt aggcttcttg gtttgatttg gcattcacct aagattctca
     2581 aacaaaaaat ggctgcaaaa aaatttgaaa aatcaaacaa attactgcat gaggggttaa
     2641 atttagttgg gaaaggtaac tttaccaagg ctgaaaaaaa actcaaagca tcgatagacg
     2701 atattcatgc ctctttgctg gctttcttga tggtatccga tatatctcag aaaaatgggg
     2761 gcgaacaaag cagagatgca tggttggaaa aagcccttgc aaaatacccc gaatcagaaa
     2821 tatatatact attgcatgca tctcaacaaa ggataaaaga gcaggattac actgctgcac
     2881 aggattacat taataccata ctggataaag atcccaataa tccaatagcg ctgaaacttc
     2941 tatctcaatg cctttacaaa actcagcagt ggagacttct tacacaaaaa gcaaagtcta
     3001 ttatcgagca agtaaaacct aatcaaatga ctgatttata tattgctaaa gcgtttacag
     3061 aaagcgctaa agaactcgtg aaagatcagc aacaaatgga aaccttatgg cacaaagtgc
     3121 cctactcctt gacaaataac gttttgattt tgactacaaa aattgaatca atgatgctta
     3181 atggccaggt aaatgctgca gaaaaagaac taagaaagca tattccaaaa acatgggata
     3241 aaaaattaat cgcccttttt gccaagcttg aaagtccaga tctttcaaaa ctgagtaaac
     3301 aaatggatcg ttggcttgtg gatagacctc gcgatgcaaa cctatggcaa gctgcaagtc
     3361 atttagcaaa gcgcgatgag ctttggaaac aagcaaaaca atttattgaa agatcgattg
     3421 aaattaatga aaatccattt gcttataatc aactaggttt tattttgctt gaattaggac
     3481 aacaagacgc tgcgatcaaa gcatttgagc aagccatgaa tctaaataac gactgacaca
     3541 tcgattcgat aaccgtttaa ttgacttaaa agatcgaaaa agaaattgat actcaagccc
     3601 acctcatgaa taagaatgct gaacagttga aaaagaattt tcttcaccac ttagagacag
     3661 aaaaaagatt ctctgttaac acaataacaa gctatcaaag agacttggcc agccttgaaa
     3721 gttattgctt tgataaccag attaacagtt ggcaagaatt aaagcctcat cacttgagag
     3781 ctttttcctc gaaaattttt aaccgtggat tagggcccag aagtattcag aggcgcctgt
     3841 caggtattcg tagttttatg aactatctat tgagagaaaa tttgataaaa tccaaccctg
     3901 ctaacggcat tagaacgccg aaagcaccca aacgtttgcc cgatgtgctg gatgttgatc
     3961 aggttaatca gttattaaac attaaaacaa ccaatccaat tagtctcagg gataaagcta
     4021 ttttggaatt gttgtattct tcaggattaa ggctttctga gttggtttct ttaaatccaa
     4081 tcgatttaaa cttgaaagac aaaagtatga ctgttcaagg taaaggtgag aaaaccaggt
     4141 tgctaccgat aggtcagaaa gcaatagaag ccgttaatca gtggttagag gttagagtgc
     4201 aacttgctca tatggatgaa gaagcattat ttgtaagctt aaaagggagg cggttaagtc
     4261 aacgtgcagt ccaatcaaga attgatcact gggctaagca aactggtatg caacaaaata
     4321 tttatccaca tttattaagg cacagttttg ctacgcatct cttggaggct agtggcgatt
     4381 taagagcaat acaagaacta ctgggacaca aagacatcag caccactcaa atttataccc
     4441 acttggattt taaacaccta gcggagacgt atgacaagac ccatcctaga tctggaaaaa
     4501 aatgaagcaa gatcttaata aaaattttga tttcggatat gagcgcgtaa ccgaagaaga
     4561 aaaaacagat aaagtgggtg ctgtctttga ttctgtttcc caaaaatatg atcttatgaa
     4621 tgatctaatg tctctgggcc tacatagatt ctggaaacga tttgcaatga tgcatactgg
     4681 attgattgaa ggaatgtcag cactcgatgt tgcaggtgga accggggatt tagcttcaag
     4741 cttgtgccaa caagtgggca agaaaggcac ggttgtatta actgatatta acttcaacat
     4801 gctcatcaac ggccgttcca agcttctgga ccagggcaaa ctaaatcaga tcaatttaat
     4861 tcaatccaat gccgaatcat tgccttttgt taatgattcc tttgattgca taactattgg
     4921 atttggatta agaaatatca ccaacaaaga aaaagctctt gcgtccatta tgcaagttat
     4981 caagcctggt ggacgactgt tgattttaga attttcaaaa cccaatgaac tgatcagccc
     5041 attctatgat ttttattcct ttagtgtgct acccaagctt ggtgattgga tggtcaatga
     5101 tgctgatagc tatcaatatt tagcagaatc gatacgaatg catccagacc aaaagaaact
     5161 taaagaaatg atggaaacaa tcggtttttc caattgtgaa tattttaatt tgaccggtgg
     5221 tattgtggct ttgcatatag ggtataaaat ttaacttttt tataaagtaa ccatgcagca
     5281 attcaagata ttagcccgat tactccatat tcataagata ctggttgatt accgattgga
     5341 tgatcttata cgagacattc cctatttaaa gccattgcaa tggttatttt atttctcacc
     5401 caaaagatgg ttgtctaata aaaatcaatt acctagagcc atccggatta gaagggcgct
     5461 ggagaaactc gggcccttgt atgttaaatt tggacaatca ttgtccacta gacctgatct
     5521 gttgcctgaa gatattgcaa atgaattaac tcagcttcaa gataatgttc cagccttctc
     5581 atcagaagca gccgaacaag aaatagaaac cgcctttgga cgttcggcac aagaggtttt
     5641 taaatcattt aattctgatg cttttgcttc agcttctgtt gcccaggcac atcttgcaga
     5701 gcttcaatca ggggaagaag tggtggtaaa aatcctaaga ccaggaattt tagatgccat
     5761 cgaaaaagac atgcagatcc tgtttatctt agccaaattg atagagggct attccaaaga
     5821 aggcaaacga ttaagaccct tggaagtagt cagtgattat caaaaagttg ttcataatga
     5881 attggacttg atgagggaag ctgcaaactg tgcccaaata gggagaaact ggaaggattc
     5941 tgacattatt catgttccta aagtttactg ggattattgt cgacctaatg tt
//
LOCUS       DASCTH010000190         3167 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000303324, whole genome shotgun sequence.
ACCESSION   DASCTH010000190 DASCTH010000000
VERSION     DASCTH010000190.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000303324"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="QGH35_04030"
     CDS             <1..294
                     /locus_tag="QGH35_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020029510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81736.1"
                     /translation="GLQVVRRAGEYAVTKPGREMLFTTVDRDTRFKAKSVIDVVVYRG
                     GDIFWAWAFTGLTQLLGFGLASIAFVGAVIASAWSLLAFKLGRYFDSHQINEN"
     gene            complement(291..830)
                     /locus_tag="QGH35_04035"
     CDS             complement(291..830)
                     /locus_tag="QGH35_04035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005283815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJL81737.1"
                     /translation="MENGMHKKIIRLLISLICPFLIIGPSLADINIGQKAPTFNLQDQ
                     NGTWHKLEDYKGQWVVLYFYPKDGTPGCTTEACNFRDNIFEFQKLNTQILGVSLDNVK
                     SHQEFSEKYSLPFPILADVDKSCAENYGVLGKFMLMTIAKRQSFIINPDGEIVKHYKK
                     VDSDIHSAEVINDLKQLGS"
     gene            892..2040
                     /locus_tag="QGH35_04040"
     CDS             892..2040
                     /locus_tag="QGH35_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013862.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HJL81738.1"
                     /translation="MSDNRLVKKIGLILPGGGARAAYQVGVLKAISAILHNGTQFPFK
                     VISGTSAGSINAALLAAEANKFHQSSIAHLIDVWANFEINQVYRTDPLTMLRSSILWF
                     LTIISGGFLISNPKSLLDNQPLRKLIKEHIQFSNIEENLNQSNFESLAITSASYKSRK
                     SCTFFQSSNVSTNWQRFLREGKATKINVDHLMASVALPLIFPAVQIDNEYYGDGAMRQ
                     ATPLSPAIRLGAEKLLIVSTHETGERLPTSDHHDRYPSFEKIAGYTLDALFADGLQSD
                     IERLDRINQIIKNTKGVEIKTDKKLMKHLDYLIVAPSEDLNDLAQKHYKCLPLTIRAL
                     LKGLGFSKDTKSEMLSFLLFESPYTQELIELGYRDGMGKKEEIINFIE"
     gene            complement(2049..>3167)
                     /gene="mgtE"
                     /locus_tag="QGH35_04045"
     CDS             complement(2049..>3167)
                     /gene="mgtE"
                     /locus_tag="QGH35_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HJL81739.1"
                     /translation="EMDASELIAVTQDMELDDLADLLIDIPEAITEQIITALDQQDQV
                     RLESVMSFEENTAGGLTNPRIVSVRRGITIEVLIRYLRKLRKLPEDTNYIYIINKHNE
                     YLGAVKLVDLFVEDKGKNIEDIMDESVKPFTAETDSHQVSLDFQDLDIISAPVVDDQN
                     KLLGQITIDDVVDVIQDQANSEIFNMAGLDDEDDIFAPVLVSSKRRAVWLGMNLVTAF
                     IVAGAVGLFKEILGQIVILAVLMPIVASMGGVAGNQTLILVIRGIATKKIQRSNAIKL
                     LIKEGSVALLNGLTWSIVVSVLAVVLFQTPWNIGYIIGAAMLLNILASAIAGVSIPFL
                     LKKVGIDPALAGGVMMTTLTDVLGFITFLGLATLFLLE"
BASE COUNT          985 a          596 c          582 g         1004 t
ORIGIN      
        1 ggattacaag ttgtaaggag agcaggagaa tatgcagtga ctaagccagg cagagaaatg
       61 ttatttacaa ccgtagacag agatacccgt tttaaggcaa aaagtgtcat cgatgttgta
      121 gtttatcgag gtggagatat tttttgggcg tgggccttta caggtctgac tcaattgttg
      181 ggattcgggt tggcatcgat tgcatttgtt ggtgccgtga ttgcttctgc atggtctcta
      241 ctagctttta agctaggaag gtattttgat agtcatcaaa tcaatgaaaa ttagctacct
      301 agttgcttca aatcattaat tacttctgca gagtgaatat ctgaatcgac ttttttataa
      361 tgtttaacaa tttcaccatc tggattgatg ataaatgatt ggcgtttagc gattgtcatc
      421 agcataaact tccctaaaac gccataattc tccgcacaac tcttatctac gtcggcaaga
      481 ataggaaatg gaagactgta cttttcgcta aattcttgat gagatttaac attatccaaa
      541 ctgacgccta atatttgggt attcagtttt tgaaattcaa aaatattatc tctgaaatta
      601 catgcctcag tagtgcagcc tggtgtgccg tcttttggat aaaagtataa taccacccat
      661 tggcccttat agtcttcaag cttatgccac gtaccgtttt ggtcctgtag attaaaagtt
      721 ggtgcttttt ggccaatatt gatatcagcc agagatggtc caatgatcag aaaaggacag
      781 atcaatgaga ttaacagtct aataattttc ttatgcattc cgttctccat aaaaacgtat
      841 aaaataatat tacgttataa aataagatgc ttcacgatta tattctaatt tatgtcagat
      901 aatagactcg ttaaaaaaat tggtttgatt ttaccgggag gaggcgcaag agcagcttat
      961 caggtaggtg tgctcaaagc tatttcagca attcttcaca acggaacaca atttcctttc
     1021 aaggtcatta gtggaacttc cgccggtagc atcaatgcgg ctttgctcgc agcagaagcg
     1081 aataagtttc atcaaagcag catcgcacat ctaattgatg tttgggctaa ttttgaaata
     1141 aatcaggtct atagaactga tcccttaacg atgcttagga gtagcattct ctggtttctc
     1201 actattattt cgggggggtt tttaatttcc aatcctaaat ccttgcttga taatcaacct
     1261 ttaagaaaac ttataaaaga gcatatccaa ttttcaaata ttgaagagaa cctgaatcaa
     1321 tctaattttg aatctttagc aattacatca gcaagttaca aatctagaaa atcctgtact
     1381 tttttccaat ccagcaatgt cagcactaat tggcaacgat ttcttcgaga gggtaaagca
     1441 accaagatta atgtagatca tttgatggct agtgttgctc taccacttat ttttcctgct
     1501 gtccaaatcg ataatgaata ttatggagac ggggcaatga ggcaagccac tccattgtca
     1561 ccagcgatta gactaggtgc tgaaaagcta ttaattgtga gtacccatga gactggtgag
     1621 agattgccaa cttctgatca tcatgatcga tacccttctt ttgaaaagat agcaggatat
     1681 acgcttgatg ctttgtttgc cgatggattg caatcagata tagaaagatt agaccggatt
     1741 aatcaaatta taaaaaatac aaaaggtgta gagataaaaa cggataagaa attgatgaaa
     1801 catttagact atctgatcgt agctccatct gaagatctta atgatctagc tcaaaaacac
     1861 tacaaatgtt taccacttac cattagggct ttactcaagg gcttaggctt ctcaaaagat
     1921 actaaatcag aaatgcttag ctttttgctc tttgaatcac cttacactca agagcttata
     1981 gagctagggt atcgtgacgg tatgggaaaa aaagaagaaa tcattaactt catagagtag
     2041 cttctttttt attctaaaag aaacaaagta gccagaccta gaaaggttat aaaacccaac
     2101 acgtctgtta aggtggtcat catcactcct ccagctaagg caggatcaat acctactttt
     2161 ttcaataaaa atggaattga aactccagca atagcagatg caagaatatt tagtaacatt
     2221 gctgccccga ttatataccc aatattccaa ggagtctgaa ataacaccac cgctaaaact
     2281 gaaacaacaa tggaccaggt taaaccattc aataatgcaa ctgaaccttc tttaattagt
     2341 agcttaattg cattggatcg ctggattttt ttcgttgcta tacctctaat gactaaaatt
     2401 agtgtttgat ttcctgctac accacccatg cttgcaacta taggcattaa aaccgcgaga
     2461 ataacgattt gtccaagaat ttctttaaag agcccaactg caccagcaac aatgaatgcg
     2521 gtaaccaaat tcattccaag ccatacagcc cttctttttg agcttaccag gacaggagca
     2581 aatatgtcgt cctcatcatc tagtccagcc atgttgaata tttcactatt tgcttggtct
     2641 tgaatgacat ccactacatc atcaatggta atttgtccca agagtttgtt ttgatcatcg
     2701 acaactggtg ctgagattat atctaaatcc tgaaagtcta atgatacttg atgagaatct
     2761 gtctctgcag taaatggttt tactgattca tccataatat cttctatatt ttttcctttg
     2821 tcttcgacaa ataaatcgac aagtttgacc gctccaagat attcattatg tttattgata
     2881 atgtaaatat aatttgtatc ctcagggagt tttcttagtt ttctcagata tcgaattaag
     2941 acttctattg ttattcccct acggactgag acaatgcgtg gattggttaa gcctcctgcg
     3001 gtgttttctt cgaaagacat tactgactca agtctgactt gatcttgttg atccaaggct
     3061 gtaatgattt gttctgtaat tgcttctggt atatctatta agagatcagc taaatcatcc
     3121 aattccatat cctgggttac tgcgatgagt tctgaggcat ccatctc
//
LOCUS       DASCTH010000191         2055 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000304788, whole genome shotgun sequence.
ACCESSION   DASCTH010000191 DASCTH010000000
VERSION     DASCTH010000191.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000304788"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="QGH35_04050"
     CDS             <1..219
                     /locus_tag="QGH35_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HJL81740.1"
                     /translation="AVIGSKIIARGNVRAMRKNVTAKCYGGDVTRKKKLLEKQKAGKK
                     RMKQFGSVEIPQEAFLIMMKKGSSLQKK"
     gene            279..1019
                     /gene="lepB"
                     /locus_tag="QGH35_04055"
     CDS             279..1019
                     /gene="lepB"
                     /locus_tag="QGH35_04055"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HJL81741.1"
                     /translation="MIFDFQAILFLITMVFGAIWLVGHFFLEKDSNVVEFSRGLFPLL
                     LIVFILRAFVFEPFRIPSGSMMPTLIKGDFILVKKYAYGLRWPITNKVFLSTSKPERG
                     DVVVFNLPSDPSIKYIKRLVGLPGDKVIYRDKQISINGELIPNKYQSVYKHPRQYGAH
                     VYEEDFAGKKHLMLLVPTKKGLEGQYTVPSDHYFLMGDNRDNSKDSRYSSPGFVSDTY
                     LVGTATKIWFNWDFENTPNWNRVWQTIE"
     gene            1050..1727
                     /gene="rnc"
                     /locus_tag="QGH35_04060"
     CDS             1050..1727
                     /gene="rnc"
                     /locus_tag="QGH35_04060"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010382304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HJL81742.1"
                     /translation="MQKTDLWCEKKFGYSFRDKILLKTALTHRSFSGEHNERLEFLGD
                     AVLDLVISEYLFQSFTGQTEGFLSRARSNLVKGDVLANIAKELNVDQHLILGKGEISS
                     GGRQRLSLLSNALEAIIGAVFLDGDYEAVKKVVLKIFNSRIKNLNLDSEHKDHKSVLQ
                     ERLQEMNKELPVYSLIRKDGKQHEQIFFVECLIKSEGIATQATGNSIRLAEQKAASKA
                     LKLMKNG"
     gene            1720..>2055
                     /locus_tag="QGH35_04065"
     CDS             1720..>2055
                     /locus_tag="QGH35_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014036.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HJL81743.1"
                     /translation="MVRDNFHSGYVAVIGRPNVGKSTLVNALVGEKVSIVTAKPQTTQ
                     MNILGIAHLPDAQIIFIDTPGLLSGRQKKLNPKQINKETRNALAMADLALIVVEANRW
                     KKDDNDLIQK"
BASE COUNT          683 a          346 c          418 g          608 t
ORIGIN      
        1 gctgtgatag gttcaaaaat aattgccaga ggtaatgtta gagccatgag gaaaaatgta
       61 accgcaaagt gttatggcgg tgatgtcact cgaaaaaaga aattgttaga aaaacaaaaa
      121 gcaggtaaga aaagaatgaa acaatttgga tctgtagaaa ttccacagga agctttcctc
      181 atcatgatga aaaaaggctc ttcacttcaa aaaaaataat caccattaat tcataattga
      241 gtcatttttt tacgatcaat gactttataa tgtgctttat gatttttgat tttcaagcaa
      301 ttttatttct aattaccatg gttttcggag ctatttggct tgttgggcat ttttttctag
      361 agaaagattc aaatgtggtt gaattttccc gaggattgtt cccactgttg ctcattgttt
      421 ttattttaag ggcatttgtt tttgaaccat ttcgtattcc gtcaggttca atgatgccaa
      481 ccttaatcaa gggagacttt attcttgtca aaaaatacgc atacggtctt agatggccga
      541 taaccaataa agtatttctc tcaacttcca agccagaaag aggggacgtt gttgtattca
      601 acctgccatc tgacccgtct attaaataca tcaaacgttt agtcggatta cctggtgata
      661 aagttattta tcgagacaag caaattagca tcaatggaga gttaatacct aataaatacc
      721 aatccgtata taaacatcca cgccaatatg gagctcatgt ctacgaggaa gattttgcag
      781 gcaaaaaaca tctgatgtta cttgtcccta caaagaaggg cttggaaggg caatacactg
      841 ttcctagtga tcattatttc ttaatgggag ataacaggga caatagcaaa gatagccgtt
      901 actctagtcc tggatttgtt tcagatactt acctggttgg cacggcaaca aaaatttggt
      961 ttaactggga ttttgaaaac acgccaaatt ggaatagagt ttggcaaaca attgaatgac
     1021 ggaattaatc aagtattaca aatatcgcca tgcagaaaac tgatttgtgg tgtgagaaga
     1081 aatttgggta cagttttaga gataaaatac ttttaaaaac tgcactgact caccgcagtt
     1141 tttccggtga gcataatgag cggcttgaat ttcttggaga cgctgtttta gatcttgtga
     1201 ttagtgagta tctttttcaa tcttttaccg gtcaaacgga gggtttttta agtagggcca
     1261 gatccaactt ggtaaaagga gatgttttag caaacatagc caaagagtta aacgttgatc
     1321 aacatctaat tttgggaaag ggtgaaatat catcgggtgg acgtcagcgc ctatcacttt
     1381 tatccaatgc tcttgaagca atcatagggg cagtattttt agatggagac tatgaggcag
     1441 tcaaaaaagt agttctgaag attttcaatt ctagaatcaa gaatttaaac ctagattccg
     1501 aacacaaaga tcataaaagt gtcttgcaag agagattgca ggaaatgaat aaagaactgc
     1561 cagtttattc actaattcgt aaagatggaa agcagcatga gcaaatattt tttgttgaat
     1621 gtttaatcaa atcagaaggt attgccaccc aagccactgg gaacagcatt cgtcttgctg
     1681 agcaaaaggc agcttccaag gctcttaagc tgatgaagaa tggttagaga caattttcac
     1741 agtggttatg ttgctgttat aggaagacca aacgtaggga aatccacctt agttaatgca
     1801 ctcgttggtg agaaagtttc cattgtcaca gccaaacccc aaacgacaca aatgaacata
     1861 ttaggaattg ctcatcttcc cgatgctcag attatcttca ttgacacccc tggtttactt
     1921 tctgggagac aaaaaaaatt aaatccaaag cagattaaca aagaaacaag gaatgcctta
     1981 gccatggccg atcttgcttt aattgttgtg gaagctaacc gctggaaaaa agatgataat
     2041 gatttgatac aaaag
//
LOCUS       DASCTH010000192         2298 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000306839, whole genome shotgun sequence.
ACCESSION   DASCTH010000192 DASCTH010000000
VERSION     DASCTH010000192.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000306839"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="QGH35_04070"
     CDS             complement(<1..749)
                     /locus_tag="QGH35_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024579.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81744.1"
                     /translation="MIKNFSENRKIMALILGCLSLLIHLIIAPVALGEPLPAYHPLKE
                     ERVVIERANKTGEFGGAWSSNVGKKFEAVSDYFGNGELEQAMTVLQAMLTWNLSKYER
                     AYVYQFMGFVYVQQNNIEKAIEVFKQCIQLDSLSTFQHQSTLFNLASLYGAEENWDLT
                     IETLMQWFQYEVDPVPEGYIMTGIAYFQKGTPLTALPYIHTANMKSIKPHENWFQLEL
                     AILFTNKQFDEAIEVLKKMATHWPEKEKYWET"
     gene            complement(754..1392)
                     /locus_tag="QGH35_04075"
     CDS             complement(754..1392)
                     /locus_tag="QGH35_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HJL81745.1"
                     /translation="MTSQQRYLTSLVCGFVISIGLFFLMQSLIDSGDRRTKSLSEGQI
                     MDFIRIQEDETLSLKDRRKPKKPVPPKEPPPPPKLTLQKQAKPIFNKIKIEIPNIDLP
                     SISGGGPFLGNWADNPAAEGDVMPIVRIEPQWPREALVAGIEGYVVVEVTIAADGSVK
                     EAFVTESNPKRLFDRNVIRAVLKWKFKPRIINGVAVERKAVQRLDFLLSNIE"
     gene            complement(1423..1827)
                     /locus_tag="QGH35_04080"
     CDS             complement(1423..1827)
                     /locus_tag="QGH35_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HJL81746.1"
                     /translation="MKFRHKRREEEESEINITPMMDIVFIMLIFFIVTTSFIKETGID
                     PNRPEAETAKRAELGNILIAIAPNDRIWMNKAPVELEAVKIMMELAHAENPESSVVVV
                     ADELASTGLVLDVMDQIRLSGISKISLSAKEK"
     gene            complement(1827..2228)
                     /locus_tag="QGH35_04085"
     CDS             complement(1827..2228)
                     /locus_tag="QGH35_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013134374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HJL81747.1"
                     /translation="MKRKYKRKKDESEINITPMLDIVFIMLIFFIVTTSFVKEISLEV
                     NRPSKTPIKEEKRSEVISIRISKEGQVFVQDRMVDLAAIRVNVEANLALKPKASVVVV
                     SDREADAGFLVRVIDQSRLAGAKNVSLAAQK"
BASE COUNT          667 a          493 c          393 g          745 t
ORIGIN      
        1 gtttcccaat atttctcttt ttctggccaa tgtgtggcca tcttcttaag cacttcaatc
       61 gcctcatcaa attgcttatt tgtgaacaaa attgccagtt ccaattgaaa ccagttctca
      121 tgtggtttga ttgatttcat atttgcagtg tgaatatagg gaagagcagt caatggagta
      181 cctttttgaa agtaggcaat gccagtcatg atatatcctt caggcactgg atccacctca
      241 tactgaaacc actgcatcaa ggtttcgatg gttaaatccc aattttcctc tgcgccatac
      301 aaactggcta aattaaatag tgtgctttga tgttgaaatg ttgatagaga atccaattgt
      361 atgcattgtt tgaaaacttc aatagccttc tcaatattat tctgctgaac ataaacaaaa
      421 cccataaatt gatagacata ggccctttcg tacttggata gattccaagt gagcatagct
      481 tgcagtacag tcatggcctg ctcaagttca ccattaccaa aatagtcgga aactgcttca
      541 aactttttac caacatttga ggaccaagca ccaccaaatt caccagtttt atttgctctc
      601 tcaataacca ctctttcttc tttcaatgga tggtaagcag gtagaggttc tcctaaagcg
      661 actggggcaa taataaggtg aattagcaaa ctcaggcatc ccaggattag agccattatc
      721 tttctatttt cagaaaagtt ttttatcatc gagttactct atgtttgaga gtaaaaaatc
      781 caatctttgg acggcttttc tttcaactgc aaccccatta ataattctgg gcttaaattt
      841 ccattttaag accgctcgga taacattcct atcaaacaac ctttttggat tagattccgt
      901 gacaaatgct tctttaacag aaccgtctgc agcaatcgtc acctcaacca ctacataacc
      961 ttctatgcct gcgaccaagg cttctctggg ccattgaggc tcaattctaa cgataggcat
     1021 aacatctcct tcggcagcag gattatccgc ccaattgccc aaaaatggac ctccaccaga
     1081 aatacttggt aaatcgatat taggtatttc aattttgatt ttgttaaaaa ttggtttcgc
     1141 ctgtttttgg agagtcaact taggaggtgg aggcggttct ttaggtggta caggtttttt
     1201 gggtttgcgc ctgtctttta aagataaagt ctcatcctct tggattctaa taaaatccat
     1261 aatctgacct tcggataaag atttagtgcg tcgatcacca gagtcgatta gcgattgcat
     1321 cagaaaaaaa agtccaattg atataacaaa accacacacc aatgaagtca ggtatctctg
     1381 ctgtgatgtc attttcaaaa caatctcact catagctaat agctattttt ccttagctga
     1441 caaagagatt ttagagatac cactaagtct aatttgatcc atgacgtcca atactaaacc
     1501 ggttgaagcc aattcatctg ctacaaccac gacactactc tctggatttt ccgcatgtgc
     1561 taactccatc attattttta ctgcttctag ctcaacaggg gctttattca tccaaatgcg
     1621 atcattagga gcaatcgcaa tgagaatatt gcccagctca gcccgctttg ctgtctcagc
     1681 ttcgggacgg tttgggtcaa tcccggtttc cttaataaat gaagtagtga caataaaaaa
     1741 tatcaacatg atgaagacta tatccatcat gggagtgata ttgatttcac tttcctcttc
     1801 ctctcttctt ttatgtctga atttcattat ttctgtgctg ccaatgaaac attttttgca
     1861 cctgctaaac gtgattgatc aatgactctg accagaaaac cagcatcggc ttctcgatca
     1921 gatacaacta ctacggaagc cttgggttta agtgctagat ttgcttcaac attgacacgg
     1981 attgcagcca aatcaaccat tctatcttga acaaaaacct gaccttcttt tgagattcta
     2041 atcgagataa cctccgatcg tttttcctct ttgataggag ttttactggg gcgatttacc
     2101 tccaatgaaa tttctttaac aaaggatgtt gtgacgataa aaaaaatcaa catgatgaaa
     2161 acaatatcaa gcatgggtgt gatattgatt tcactttcat ctttctttct tttatatttt
     2221 ctcttcattt tgactatttt ctaatcaaaa gatctgccaa ttcatggact tgatcatctg
     2281 ctgttctttt cagaagat
//
LOCUS       DASCTH010000193         1604 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000308349, whole genome shotgun sequence.
ACCESSION   DASCTH010000193 DASCTH010000000
VERSION     DASCTH010000193.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000308349"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            263..>1604
                     /locus_tag="QGH35_04090"
     CDS             263..>1604
                     /locus_tag="QGH35_04090"
                     /inference="COORDINATES: protein motif:HMM:NF018012.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HJL81748.1"
                     /translation="MARKNKRRSRRSKTSGDHDSIQPVVTKGMEGGKYQPLSDHEIEQ
                     IHQTTLDVLENIGMANPLPQLKEVALEQGCNFTDQGRLCFPRSLVEDVIARAGRDFIM
                     YGRDPKHDIEMSDKKVHLYGGGEAVTILDLGATSYRPSIINDVYDIARMVDYLEHIHS
                     YSRLVVATEFSDLLKTDINTAYASVVGTAKHTALTFANGKHIKPTIEMLDLIAGGEGK
                     FLKRPFCHGGGCTVVSPLRYGNDNCEVAIASIEMNAPVWVVIAPQSGATAPAALAGSL
                     VQVLAETLASLLLIDLIKPGHPVIFGPWPFVTDLRTGSFSGGSGEEALMSAASAQITN
                     HYGLASSVGAGMTDSKLPDAQAGYEKGITVAMAALAGCNNVSESSGMMASLMGCSYES
                     LVIDNDMLGMIMRVVRGIEVNEETLSYRAIKETVEGEGHFLRDPQTLSLMKSEYL"
BASE COUNT          487 a          314 c          364 g          439 t
ORIGIN      
        1 acatcaaagc ctttttttgc aagattgata gccgtattta ttcctgtaaa accagcgccc
       61 actatgcaaa catctgctga aatactttgc tctagcactg ggaattcaag agcatcattt
      121 atggtatcag cataataaga gttggggtga tctagatcta tattctcaat attattcatg
      181 gcaataaatc ataaaatgat aattattgat atctgaagaa cagtataata tctaagtata
      241 aagcaattta ggaaacgata gtatggcaag aaagaacaaa cgtcgatcca gaagaagtaa
      301 aacctctggt gatcatgatt caattcagcc tgtagtcacg aaaggaatgg aagggggaaa
      361 ataccagcct ctcagtgatc acgagataga acagatacac caaacaacat tagacgtcct
      421 agaaaatata ggtatggcca accctttacc tcaattaaag gaagtggctt tagaacaggg
      481 atgtaatttt acggatcaag gacgcttgtg ttttccaaga tccttggtgg aagacgtgat
      541 tgctcgtgcc ggaagagatt ttattatgta tggtcgtgat ccaaaacatg atattgaaat
      601 gtctgacaaa aaagttcatc tctatggcgg tggtgaagct gtcaccattt tagatctggg
      661 agcaacaagc tatcgtccat caatcattaa tgatgtttat gatattgctc ggatggttga
      721 ttatctggaa catattcata gttattcaag acttgttgta gccacagagt tttcagatct
      781 actgaaaaca gatatcaata ccgcctatgc cagtgttgtt ggcaccgcaa aacatactgc
      841 tctgactttt gccaacggca agcacataaa accgactata gaaatgctgg atctaattgc
      901 aggaggtgaa ggtaagtttt taaagaggcc tttttgccat ggtggtggtt gcacggtagt
      961 ctcgccatta cgatacggaa acgataactg tgaagttgcg attgcttcca tcgaaatgaa
     1021 tgctccggtt tgggtagtga ttgcaccaca atcgggtgca acagcaccag ccgctctggc
     1081 aggctctcta gtacaagtac tagcagaaac gttggcaagt ttattactca ttgacttaat
     1141 caagcctggt catcccgtaa tctttggtcc ttggcctttt gttacggatc tcagaaccgg
     1201 gtctttttct ggtggcagtg gtgaagaagc acttatgagt gctgcatcgg cacaaattac
     1261 caatcattat ggactcgcca gctcagttgg tgctggtatg accgattcca aactacctga
     1321 cgctcaagca ggctatgaaa aaggcataac agttgcgatg gcagcattgg caggctgcaa
     1381 caatgtttct gagtcatctg gaatgatggc aagcctaatg ggatgctcct atgaatcttt
     1441 ggtcatagac aatgatatgc tgggcatgat catgcgggtg gtcagaggca tcgaagtgaa
     1501 tgaggaaacc ctctcctatc gagcaatcaa agaaacagtc gaaggcgaag gacatttctt
     1561 aagggaccct caaacgctgt ctctgatgaa atctgaatac ctct
//
LOCUS       DASCTH010000194         1897 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000310850, whole genome shotgun sequence.
ACCESSION   DASCTH010000194 DASCTH010000000
VERSION     DASCTH010000194.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000310850"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1897
                     /locus_tag="QGH35_04095"
     CDS             <1..>1897
                     /locus_tag="QGH35_04095"
                     /inference="COORDINATES: protein motif:HMM:NF039587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HJL81749.1"
                     /translation="AQTLDEYSVVIPFSLEQPRYSTKTLVYSDEFGLYFGFINQQPEE
                     SRDRRKSNRDMLHGDYDRNIVAIDFDNKGNTGYMLGISLGGSLLDFAVTNETQTDGDW
                     DGEWYAKTSESKENWYSEFFIPWALVSMKKQEGSTRKIRVGFSRGVQHLSQTYGLPEI
                     SPMRTKFLSLFIPIEVNQFQQGRLDFYPYIVASDDYENDNSAYQIGTDIFYTNGEGSE
                     ITATINPDFGQIESDSLVVNYSARETFYSDKRPFFTQSHSLFDIHENWYPGFELYSIV
                     HTRRIGSAPDYDCSQYGLANGGSNEEELECELSEEPINDIDAAIKYTRLGEQTDIGFF
                     GAFEHDEDFSEGKDFYAFRTLHKSGYHTIGHMITHAKSGAINRDATVNVMDYKYKPSD
                     VITLQNFVFHSDSNQEEGGLGVRSVVTFEHNKNWRNGFEIYYFDEHLDFNDMGYMWRN
                     DLLAGGGFVRYENTDFGENSRVLSRTYNLDFFDKNNSDNDNLKEFISLFYSQRNKNRS
                     SFNCNLFYSPHGNDDEVTRGMGMAPFIQLRKELDFDCGYRSGMRGKWQWNLTLSSGTG
                     DYFVFSADRKSIRTGVGYFPQDNLKFNFHLHQTKTDNWFIWTDVNQYASYESKNTRIS
                     TDMKWYP"
BASE COUNT          619 a          305 c          400 g          573 t
ORIGIN      
        1 aagcccagac acttgatgaa tattcagtag ttataccgtt cagcctagag caacctagat
       61 acagtacaaa aaccttagtg tattcagatg aattcggtct ttattttggt tttatcaacc
      121 aacaaccaga agagtcaaga gatcggagaa aaagtaatag agatatgcta catggtgact
      181 acgatcgcaa tatcgtggct attgattttg ataacaaagg taatacgggc tatatgctgg
      241 gcatttcttt aggcggatcc ttgttagatt ttgctgttac taacgaaacc cagactgatg
      301 gtgattggga tggtgagtgg tatgccaaaa catcagaatc taaagagaat tggtattcag
      361 aattttttat cccctgggcg ttagtttcga tgaaaaaaca ggaagggtct accagaaaaa
      421 ttagagttgg tttcagtaga ggagtacaac acctgtccca aacttatggc ttaccagaaa
      481 taagcccaat gagaacaaaa tttctttcac tctttatccc aatagaggtt aatcaattcc
      541 aacaaggtcg attggatttt tatccgtaca ttgttgctag tgatgattat gaaaatgata
      601 attctgccta ccaaattggt acagatatct tttataccaa tggtgaaggc agtgaaataa
      661 cggcaacgat caatccagat tttggccaga ttgaaagtga tagtctagtt gtgaattatt
      721 ctgccagaga aacattttat agtgataaaa ggcctttttt tacacaaagc cactccctct
      781 ttgacattca tgagaactgg tatccagggt ttgaactcta ttccatcgtt cacaccagaa
      841 gaataggttc tgctccagac tatgactgtt ctcaatacgg tttagctaat ggaggcagca
      901 atgaagagga attggaatgt gaattgagtg aggagccgat caacgatatt gatgccgcta
      961 tcaaatacac gcgattaggt gaacaaaccg acattggttt ttttggggct tttgaacacg
     1021 atgaagattt tagcgaaggg aaagattttt atgcttttag aactctccat aagtctggat
     1081 accacaccat aggacacatg attacacacg ctaaaagtgg agctattaat cgcgatgcaa
     1141 cagtgaacgt aatggattat aaatacaaac cctcggatgt gattacttta caaaacttcg
     1201 tgtttcattc tgattccaat caggaagagg gaggcttagg tgtaaggagt gtggtcactt
     1261 ttgaacacaa taaaaattgg cgcaatggat ttgaaattta ctattttgat gaacacttag
     1321 attttaacga catgggttat atgtggcgca acgaccttct tgcaggtggt ggttttgtca
     1381 gatatgagaa tacagatttt ggtgaaaatt ccagggtctt gagtagaact tataatctcg
     1441 atttttttga taaaaacaat tcagacaatg ataatttaaa agaatttatc tcattgtttt
     1501 atagtcaaag gaataagaat cggtccagct tcaattgtaa tttattttat agccctcatg
     1561 gcaatgacga tgaagtcact cgaggaatgg gtatggcacc attcattcaa ttacgaaaag
     1621 aacttgattt cgattgtggg tatagaagtg gcatgagagg gaaatggcaa tggaatttga
     1681 ctctatcatc aggcactgga gattattttg tatttagtgc ggataggaaa tccattagaa
     1741 ctggagttgg ttattttcca caagataatt tgaaatttaa ttttcatttg caccagacca
     1801 aaactgataa ttggtttatt tggacagatg taaatcaata tgcaagttat gaatcaaaaa
     1861 atacaagaat ctctactgac atgaaatggt atccaag
//
LOCUS       DASCTH010000195         2759 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000311191, whole genome shotgun sequence.
ACCESSION   DASCTH010000195 DASCTH010000000
VERSION     DASCTH010000195.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000311191"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(48..1496)
                     /locus_tag="QGH35_04100"
     CDS             complement(48..1496)
                     /locus_tag="QGH35_04100"
                     /inference="COORDINATES: protein motif:HMM:NF020007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HJL81750.1"
                     /translation="MNNSWYQEPKDMLTERGLAGLDLETTGINRKIEYERPTSAAILE
                     STDNIENPDIIIDNKCRLPYHVLPDAGALRVTNINPMELMNEELSLHQLIRKIVAYFV
                     SNPNKILVTYNGATYDLIILRHSFFSSLHNPYLLSNAFEDRIHIDLYKIAQSIYCFAP
                     KSIAFFKDATGKVVLKQEKLAKENGIDPGNAHAALDDVKALLKLANLFEQQAPEIFFS
                     AIASGNKSRAVKLMTEQLYFTYGEVKYKERLAVKRTPTFICEDPTYSNQMIFFDLCFD
                     PDDYLFMTAEEISTLINKKNSPFFSIKSNSSPVILHPSISDENNLNEEKATHRASLVQ
                     ASNGFKQNVYLACDIISKKRTPWTMLSFPESQIYAQFIDKSDRLLCDAFLNSININDR
                     LEIVNQLNDDRLIDFAKRILAFEHHDCGPKLIMEYQEFEAMRLLNEGQVPWRTLQNGW
                     QSLEKEVEGNKLSPSLLKGIKEYYTLVENKVS"
     gene            1576..1755
                     /locus_tag="QGH35_04105"
     CDS             1576..1755
                     /locus_tag="QGH35_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010392638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81751.1"
                     /translation="MLSKKFFIKGFSLILMVSIVSCGQQGDLYLPEDPAKEQAQSTAL
                     NSPSLIPAEQIIINE"
     gene            1748..>2759
                     /locus_tag="QGH35_04110"
     CDS             1748..>2759
                     /locus_tag="QGH35_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014759.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HJL81752.1"
                     /translation="MNDIQSLLLVDGSSYLYRAFHALPGLTSPTGQPTGAIYGVLTML
                     QKLIKAEKPDLVGIIFDAPGKTFRHDLFPEYKANRESTPEDLVVQIEPLLTAIQNLGL
                     PLISIQGVEADDVIGTLARQAENNNIQTLIATGDKDFAQLVSERIKLIDTMKNITTDQ
                     SGVVDKFGLGPEQIIDYLALAGDPSDNVPGIPKVGPKTATKWLQKFTDINGIIKHADE
                     ITGKVGEALRTSIPDLALYQKLVTIDCNVKLEHTVHDLVIGHAQEEALFEQLNTLGLH
                     RLIKQFMIPKPEIRTQKINNYYGILTKKEFNALLSLLDKADIAAIDTETTSLNYLQAE
                     IVG"
BASE COUNT          856 a          520 c          524 g          859 t
ORIGIN      
        1 tgtgtcatca gttttttgac tttatcagta tgttcaaaat ccatcgatta actcactttg
       61 ttttctacca aagtatagta ttctttgatg cctttcaata atgaaggcga taatttgtta
      121 ccctccactt ctttctccag agactgccac ccattttgta gagttctcca gggcacttgg
      181 ccctcattta gcagtctcat agcttcaaat tcttgatact ccattattaa tttaggacca
      241 caatcatggt gctcaaaggc cagaatcctt ttagcaaaat caatcagccg atcatcgttt
      301 aactgattga caatttccaa gcgatcattg atattgatgc tgttaagaaa agcgtcacac
      361 aataaccggt cagacttatc aataaactgt gcatagattt gggattctgg aaaactaagc
      421 atagtccaag gagtcctttt tttactgata atgtcacacg ctaaataaac attttgtttg
      481 aatccattgc ttgcttgtac tagagaagct ctatgtgtag ccttttcttc attcaggtta
      541 ttctcatcac tgatgctagg atgcaatatt actggactgc tgtttgactt gatggaaaaa
      601 aagggtgaat tttttttatt gatcaaagta gaaatttctt ctgctgtcat aaataggtaa
      661 tcatcagggt caaagcaaag atcaaaaaaa atcatctgat tggaataagt gggatcttca
      721 caaataaatg ttggcgttcg tttaactgcc aaacgctctt tatacttcac ctcaccataa
      781 gtgaaataca attgttcagt catcaatttt actgctctgg atttgttacc tgaagcaatt
      841 gcactaaaaa aaatctctgg agcctgttgc tcgaataaat tggccagttt tagcaaggct
      901 ttgacatcat ccaatgccgc atgggcattg cctggatcga tgccattttc ttttgccaat
      961 ttttcttgtt tcagcaccac tttgccagtt gcatctttga aaaaagcaat acttttgggt
     1021 gcaaagcaat aaatagactg agctatttta tatagatcga tatgaatgcg atcttcgaag
     1081 gcattggata ataaataagg gttatgcaga gatgaaaaaa aagaatgcct gagaataata
     1141 aggtcataag tagcaccgtt ataggtgacc aatattttat ttgggttgct gacaaaatag
     1201 gcaacaattt ttctaatcag ttgatgtaat gataattctt cattcatgag ctccattgga
     1261 ttgatattgg tgaccctgag agcaccagca tctggtaaaa cgtgataagg caatcggcat
     1321 ttgttatcaa tgattatgtc tggattttca atattatctg tagactctaa aatagcagca
     1381 gaagttggtc gttcgtattc gatcttccga ttgatccctg tggtttctaa atccagacca
     1441 gccaagcctc tctcagttag catgtcttta ggttcttggt accatgaatt gttcatattt
     1501 tattaaccaa ttatttttat gagttcagag atgataaaat aagcactcag agttattcaa
     1561 aagttattat caaatatgtt atcaaaaaaa ttttttataa aaggcttttc tctaattcta
     1621 atggtgtcga tcgtgtcttg tggccaacaa ggtgatctat acctgcctga agatcctgcc
     1681 aaagaacaag cccaatcaac tgctttgaac tcgccttcat tgattccagc tgaacaaatc
     1741 attattaatg aatgatatac agtcactatt attagtagac ggttcttctt acctataccg
     1801 tgcttttcac gctctgcctg ggctcacatc gcctacaggt cagccaaccg gtgcgatata
     1861 tggtgtttta accatgttgc aaaaattgat aaaggctgag aagccagatt tagtaggtat
     1921 tatatttgat gcaccaggga aaacgtttag acacgactta tttccagaat acaaggccaa
     1981 ccgtgagagc acccctgagg atcttgttgt gcaaattgaa cccctcctga cagcgatcca
     2041 aaaccttggt ctgccgttaa ttagtataca gggcgttgaa gcggatgatg tgattggaac
     2101 attggctaga caggcagaaa ataacaatat tcaaacatta atagcaacag gcgataaaga
     2161 ctttgcacaa ttggtttcag aaaggattaa gttgattgat accatgaaaa atatcaccac
     2221 agatcagtcg ggcgtagtgg ataaatttgg tttagggcct gagcaaataa tagattatct
     2281 agctcttgct ggggatcctt cagataatgt tcctggtatt cccaaagtgg gcccaaaaac
     2341 agctacaaaa tggctgcaaa agttcacaga tataaatggg atcatcaagc acgctgatga
     2401 gattaccgga aaagtgggtg aagcgctcag aacttcaata cccgatttgg ccttgtacca
     2461 aaaactggtc actattgatt gcaatgttaa gcttgagcac actgtccatg atttggttat
     2521 cggtcacgct caagaagaag cattatttga acaattaaat acccttggtc tccatcgctt
     2581 aatcaaacag tttatgattc caaagccaga aataaggacg cagaagatca ataattatta
     2641 tggcatctta accaaaaaag aatttaatgc attgctgagt ttattggata aggccgatat
     2701 tgctgctatt gatactgaaa caacaagcct aaattacttg caggctgaaa tcgttggta
//
LOCUS       DASCTH010000196         4883 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000311379, whole genome shotgun sequence.
ACCESSION   DASCTH010000196 DASCTH010000000
VERSION     DASCTH010000196.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000311379"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            14..136
                     /locus_tag="QGH35_04115"
     CDS             14..136
                     /locus_tag="QGH35_04115"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HJL81753.1"
                     /translation="MDFILFHIGQWYYPAFNIADTCITIGLILFLIENYRSTNK"
     gene            215..652
                     /locus_tag="QGH35_04120"
     CDS             215..652
                     /locus_tag="QGH35_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HJL81754.1"
                     /translation="MEEVLRLKSTVEQKLKMHDLQSTRARIRIGMILFANPQHLSAGE
                     IYEQLTKQGHEISKATVYNTLNVFAKRGMIHAVDVDPERMFYDSTISPHHHFYNIDTG
                     QLTDIPVEDLCIEEIPRLPDNTEIEDLQVIIKVKNKTSTTTSF"
     gene            706..1482
                     /locus_tag="QGH35_04125"
     CDS             706..1482
                     /locus_tag="QGH35_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HJL81755.1"
                     /translation="MARHRALQLFGRSGNPAFKSKAFSERSSDFSTSEAMTLSGTVNK
                     TGLYLLLCFITAAWTWNQFYATGDPASISGYMMIGIFGGLIVALVTIFKPTAAPISAP
                     IYALLEGLAIGGISAMFEANYPGIVIQAVGLTFGTLAALLMAYKSGLIKPTENFRLML
                     FAATGGILLLYLVNIIMGFFGAQIGFIHSNGLMGIGFSLVVVAIAALNLVLDFDFIEQ
                     AAEQGAPKYYEAYGAFSLLVTLIWLYLEILRLLAKLYSRN"
     gene            complement(1709..3274)
                     /locus_tag="QGH35_04130"
     CDS             complement(1709..3274)
                     /locus_tag="QGH35_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012141955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HJL81756.1"
                     /translation="MTNLYRKSHLLGTKVKNIRKINKLTLEDLSERCSQDDPKKAPSV
                     SYLSMIENGKRLPSSDVLEQIARVFQKEPTWFLDENLDMGIPEQHQSNQHKSSTLPLE
                     PKLLYSRDLLQGAIPELLKQTGISGKQFAHLLIRSYQEQNQNRFPDLEKAADEIGNKL
                     FPVKIKDIKNLYKKHGLTIKWFKQNKVTTSAPQIENKMLLRSFFESPSEVYVNEHLKD
                     DPQRLKYDLATHLGHKILHQGDGLRSPISSGSNAFNSQHQHGRDVNSYDVLSAWHDFE
                     SSFFASALLCPKKPFHSYLIKKKHQILSAKELELSHALMMRRVTAVSNYPYWHYFEAY
                     QPGYLTAVYRANGIPLPWGNMAMVSDPCPNWAVFRILNESHVDNPQTQISVLKNENET
                     YLYCCYSIRLRDLAGNLKLYSLGIDLIPAIKNQGIDATQLLSDLVEECQQGGGEAEVH
                     SETKEVIEKISHILRIEWLKDSLSFPARIICPRSSNCQRENKCAPSRTQYKFNEIDEL
                     KENILMLKKADLI"
     gene            3376..>4883
                     /gene="aceB"
                     /locus_tag="QGH35_04135"
     CDS             3376..>4883
                     /gene="aceB"
                     /locus_tag="QGH35_04135"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HJL81757.1"
                     /translation="MEHNSIEIQGEQTSEVQEILTPTALELLQHLHRTFNSQRLELLK
                     LRKQRQQEIDDGALPEFLPHTQSIRDDNWSVAPLPDDLMDRRVEITGPVDRKMIINAL
                     NAPVKTFMADFEDSCSPTWNNIIHGQTNLRDAVNKNIEFISPDGSKTYALNEKIATLI
                     VRPRGWHLHEKNLLIDGEPVSASLFDFCLYLTTNFEQLAKNGTGPYFYLPKLENHLEA
                     RLWNDVFVMSQNKLGMQQGTIKATVLIETILAAFEMDEILFELREHSSGLNCGRWDYI
                     FSFIKKFRNRSDFILPDRDTVTMTRHFLKSYVDALIQTCHKRGVHAMGGMAAQIPIKN
                     DPEGNIEAMAKVKADKRREADAGHDGTWIAHPGLASIAMDSFDAVMKESPNQLHKLRE
                     EVRISSQDLIKVPTGDVTDTGLRHNIRVGVQYLEAWLSGNGCVPLYNLMEDAATAEIC
                     RSQIWQWITHKVKTNDDQIIDEERFTQSLQEEISKLIEERGDLSYDPEQLKVA"
BASE COUNT         1453 a          896 c          988 g         1546 t
ORIGIN      
        1 tcaaggttac gttgtggatt tcatactgtt tcacattggc caatggtatt atccagcatt
       61 taatattgca gacacttgca ttactatcgg tctgattctt tttttaattg agaattatcg
      121 ctcaacaaat aagtaaaaaa attctgcaaa ggattgatct ttataggggg ttttactttg
      181 caattcttat ctttgttact aaaatatgaa gaccatggaa gaagttttaa gactaaaatc
      241 aacggttgag caaaaactca aaatgcatga cctgcaatca acaagggcac gcattagaat
      301 cgggatgata ttatttgcta accctcagca tctttccgca ggggaaattt atgagcaatt
      361 aaccaaacaa ggtcacgaga tttcaaaagc caccgtctac aataccttaa atgtgtttgc
      421 caagagagga atgatccatg cagttgatgt ggatccggaa agaatgtttt acgattccac
      481 catttctcca caccatcact tctataacat agataccggt cagttaacgg acatcccagt
      541 tgaagatctt tgtatcgagg agataccaag attaccagat aatactgaaa ttgaagactt
      601 acaagtaatc attaaggtta aaaataagac ctctacaacc acgtcctttt aagatttcaa
      661 atgtataata tttccattat taagtaattt attgaggagc taattatggc gcgtcatcgg
      721 gcattacaat tattcggcag atctggaaac ccagcattta aaagcaaagc tttttcagaa
      781 cgttcatctg atttttcaac atcggaagcg atgaccttat ccgggacggt caataaaact
      841 ggattgtatt tgttgctttg ttttatcacc gcggcttgga cttggaatca attttatgcc
      901 acaggagatc ctgcctcgat ttcagggtat atgatgattg gtatttttgg tggattgatc
      961 gtggccctgg ttacaatttt taaacccact gctgcaccga tctccgcacc catttatgcc
     1021 ttgcttgaag gactggctat tggtggaatc tcggccatgt ttgaagccaa ttatccaggt
     1081 attgtgattc aagctgtggg ccttactttt ggaacattgg ctgctctttt aatggcctat
     1141 aaatccggtt taatcaagcc aactgaaaat tttaggttga tgctgtttgc tgcaactgga
     1201 gggatattat tactctatct ggtgaacatc atcatgggtt tctttggtgc tcaaataggt
     1261 ttcattcact ccaatggtct aatgggaata ggattcagct tggtggtggt ggccattgct
     1321 gcattgaatt tagttctgga ttttgacttt attgaacaag ctgcagaaca aggtgctcct
     1381 aaatattatg aggcctatgg ggccttctca ttactggtaa ccttgatatg gctctatctt
     1441 gaaattctca gactattggc aaagctgtat tccagaaatt gatggatgcc aatacacttt
     1501 tctttggctt gctgatcatc ggctcgctag ccattttttt ttactttgga cagtttagag
     1561 cgtcaagcaa acaacgcgat cgaaaagata gaatcgattg gacaaagtca atgtttaaga
     1621 gaatatccaa ctcgtttaaa aaaaattaac gtattattta atattgaaaa ttataagaat
     1681 atgatgtgag tgtcagtgat gaatgtcttt agattaaatc ggcttttttc agcatcaaaa
     1741 tgttttcttt taattcatct atttcattaa acttgtattg tgtcctacta ggagcacatt
     1801 tattttctct ctggcagttc gagcttcttg gacagataat tcttgcaggg aaagaaagag
     1861 agtctttcaa ccactctatt ctcaaaatgt gactgatttt ttcaatcact tcttttgttt
     1921 ctgaatgtac ttccgcttca cccccacctt gttggcattc ttcgacaaga tcgcttaaca
     1981 attgagtggc atcaatgcct tggtttttga ttgcagggat taaatcgata ccaagagaat
     2041 acagctttaa atttcctgca agatctctca accgtataga ataacaacaa tacaaatagg
     2101 tctcattttc atttttaagg acagagatct gggtttgggg attgtcaaca tggctttcat
     2161 tgagtattcg aaacactgcc caatttggac aaggatcaga gaccattgcc atgtttcccc
     2221 aaggaagcgg tataccattg gctctataaa ctgcagttaa ataacctggc tgatacgctt
     2281 caaaataatg ccaataggga taatttgaaa ctgctgtaac ccttctcatc atgagcgcat
     2341 gagagagttc cagttcttta gcagagagta tttgatgctt ctttttaata agataactgt
     2401 gaaaaggttt ctttgggcat aacagggcac ttgcgaagaa actagattca aaatcatgcc
     2461 aggcggaaag tacatcgtag gaattcacat ctctgccgtg ttgatgctga gaattaaagg
     2521 cattagaacc agatgaaatt ggcgatctaa gaccatcacc ttgatggagt attttatgtc
     2581 ccagatgggt tgctaggtca tatttaagtc tttgtggatc atcttttagg tgctcgttaa
     2641 cataaacctc agatggcgat tcaaaaaagg aacggagcag catcttattt tctatttgtg
     2701 gagctgatgt cgttacttta ttttgtttga accatttaat tgtcaggcca tgttttttat
     2761 ataagttttt tatgtcctta atcttcactg ggaaaagctt attacctatt tcatccgcag
     2821 ctttttctaa atctgggaaa cgattttgat tttgttcctg ataagaacga attaaaagat
     2881 gagcaaattg tttacctgag atcccggttt gcttcagaag ttctgggata gccccttgca
     2941 aaaggtctct actgtataaa agttttggct ctaagggcaa ggtagaggat ttgtgttgat
     3001 tcgactgatg ttgttcaggt attcccatat ctaggttttc atctagaaac caagtcggtt
     3061 ccttttgaaa gactcttgca atttgttcga gaacatctga agatggcaaa cgcttaccat
     3121 tttctatcat gcttaaatat gataccgaag gcgctttttt tggatcgtct tggctgcatc
     3181 gttctgatag atcttccaaa gtgagcttat tgatcttgcg aatattcttt actttggtac
     3241 caagtaggtg gctttttctg tacaggttag tcatttgtga aatttacatt gtgaaatttt
     3301 tactgtgaaa ttttaactaa aagtttaata aaattccatt taaatcattt taataacgct
     3361 taaacattaa taagaatgga acacaatagt atagaaatcc aaggtgagca aacttctgaa
     3421 gttcaggaaa tactgactcc aacagccttg gagttattgc aacacctcca ccgaaccttt
     3481 aactcccaac ggttggaatt gttgaagcta agaaagcaga gacagcaaga aattgatgat
     3541 ggagcactgc ctgaatttct acctcataca caatccattc gagatgataa ttggtcagtt
     3601 gctcccttgc ctgatgatct aatggatcgc agggttgaaa ttaccggtcc ggtagatcga
     3661 aaaatgatta tcaatgcact caatgcaccg gtaaaaactt ttatggctga ctttgaagat
     3721 tcctgttctc ctacctggaa taacatcatc catggccaga ctaatcttag ggatgctgtc
     3781 aataaaaata ttgaatttat atcccctgat ggttcaaaaa cctatgcctt gaatgagaaa
     3841 attgcaactc tgattgttcg acccagaggt tggcatcttc atgaaaaaaa tctattgatt
     3901 gatggtgaac cggtgtcggc ttcattgttt gatttttgtt tgtatttaac taccaatttt
     3961 gaacaattag ccaaaaatgg aaccgggcca tatttttatc ttccgaagtt agagaatcat
     4021 ttagaagcca gactttggaa tgatgttttt gtgatgtccc agaataaatt gggtatgcaa
     4081 cagggcacca tcaaggccac tgtattaatt gaaactattc tcgctgcttt tgagatggat
     4141 gaaattcttt ttgagctcag agaacattct tctggtctta actgtgggcg ttgggactat
     4201 atatttagtt ttatcaagaa atttagaaat cgctcagact tcattctccc ggatcgagat
     4261 acggtgacca tgactcgtca ttttcttaaa tcctacgtcg atgcattgat tcaaacatgc
     4321 cataaacgtg gtgttcatgc catgggtggt atggcagcac aaattccaat caaaaatgat
     4381 ccagaaggta atatcgaggc aatggcaaaa gttaaagctg ataaaaggag agaagctgat
     4441 gcaggtcatg acggaacatg gatagctcat cctgggttag cttccatcgc aatggattcg
     4501 ttcgatgcag tcatgaagga gtctccaaat caactccata aattaagaga agaagtcaga
     4561 atcagttcac aagatttaat aaaggtgcct acaggggatg taacagatac tgggttgcga
     4621 cacaatatca gagtcggcgt tcaatacctc gaagcatggt tgtcagggaa tgggtgcgtc
     4681 ccgctgtata acctaatgga agatgcagcc accgctgaaa tatgtcgatc acaaatctgg
     4741 cagtggatta cccataaagt gaaaacgaat gatgatcaga taatagatga agaacgattt
     4801 acccagtcac ttcaggaaga gatatccaag ctcattgagg agcgaggtga tctctcttac
     4861 gatccagagc aattaaaagt ggc
//
LOCUS       DASCTH010000197         2199 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000311691, whole genome shotgun sequence.
ACCESSION   DASCTH010000197 DASCTH010000000
VERSION     DASCTH010000197.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000311691"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="QGH35_04140"
     CDS             complement(<1..247)
                     /locus_tag="QGH35_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJL81758.1"
                     /translation="MSHIPIKNSLADLYRWRVKQSPNEIAFKFLDRETTYIEHDHFAN
                     KVAQGLIAEECKPNNRVAFLAKNSDYYCELLYGSLKSR"
     gene            518..>2199
                     /locus_tag="QGH35_04145"
     CDS             518..>2199
                     /locus_tag="QGH35_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81759.1"
                     /translation="MRKLFNSIFLLVIAISISTVNTFAAELEEITVTATKREISLQDV
                     PVSVSVISGEKIEKAAIHSFTQLSTYIPNFSVSENAISTIASMRGVGIGANQSFENSV
                     GLFVDGVHLAKGRQYRNGLFDIERVEVLRGPQGTLFGKNTLAGAVNVISAKAKVGEDF
                     GGRIAVASEDNEGQLVEGNIHFSPSDTFAVRFAFKDREDDGYIDNLYLGNSGPTTDET
                     LIRISGAWEPNDNLSVHFKHTDGDHVRTGNTVAMKHWEMASPPTATSGLAFLLTNMFH
                     ASLPAAVAAGNVIGYHDQNWGAAGTAGSTTNVIGKVPVGTDTQIQDTSLNIELEFGDG
                     YTFTSTTGRAEYEYVDGIDADFAAIVLVSRDDWSTYDQTSQEFQIASPADRDFSFIVG
                     TYWDSQEQDIERLIDLDGTVGGLMGMIGQPSILNIPLANLTAMGLPMFYLNGSINDPT
                     AAYLISLGVLPGTPAAYQTIFDHATRIGYWNQKTEAKAVYFQGTQQMKEDLAITFGVR
                     YVEENKHIRAGTCLGADTTGLQTCNSNAFLAAIMAASFDTYAHNFDNIPERET"
BASE COUNT          643 a          448 c          470 g          638 t
ORIGIN      
        1 ttctagactt taaggatcca tagagcagct cacaataata gtctgaattc ttagccagaa
       61 aggcgacacg gttattcggc ttgcattcct ccgcaatcag tccttgtgcc actttattgg
      121 caaaatgatc atgttctata taggtggtct ctctgtcgag gaacttaaat gctatctcat
      181 tgggtgattg tttaaccctc caacggtata ggtctgcaag actgtttttt attggtatat
      241 gactcatttg taaacgttaa gttctagata ctacacttcc ttaaagattt tttaaaaaaa
      301 aaacagctta aaaattgtta aaaaaatcag atttattata tatatcaatt acttagacta
      361 aaatatctaa aaattgcggt tattatgaac tattttcttt tgccaagtgg tctaactatt
      421 ggcattatat ctttattatt aactatcgcg atagcatgct ctggttcgta cgtttcaaaa
      481 tgagcaactg ttccatcgca gattagaggg gaaaataatg agaaaattgt ttaattcaat
      541 ctttctttta gtaatagcca ttagcatctc aactgttaat acttttgctg ctgagttaga
      601 agaaattacc gtaacggcaa cgaaaagaga gatcagtctt caagacgtgc cggtctcagt
      661 cagtgtgatt agtggtgaga aaatcgagaa ggcagctatc cacagtttta cacaactgtc
      721 tacctacatt cctaatttca gtgtttcaga aaacgcaatc agtactatcg catcaatgcg
      781 tggtgttgga attggtgcta accaatcgtt tgaaaactct gttggtttgt ttgtggacgg
      841 ggttcacttg gctaaaggac gccaatatcg taatggactt tttgatattg agcgagtgga
      901 ggtcctcaga ggaccacaag gaaccttgtt cggaaaaaat accctcgctg gagcagtgaa
      961 tgtgatttca gcaaaagcta aagtgggtga agactttggt ggcaggatag cagtcgcctc
     1021 agaagacaat gaggggcaac tagttgaagg taatatacac ttttccccca gtgatacgtt
     1081 tgcagtccgc ttcgccttta aagatcgtga ggacgatggc tatattgaca atctctattt
     1141 aggaaattca ggtccaacaa ctgatgaaac cctgatccgc atcagcggtg cgtgggaacc
     1201 caatgataat ttaagtgttc atttcaaaca taccgatggg gatcatgtac gaacaggtaa
     1261 taccgttgca atgaaacatt gggaaatggc atcgccaccc actgcaacct caggtctggc
     1321 ttttttactc accaacatgt tccatgccag tttacctgca gccgttgctg ctggtaacgt
     1381 cattggctat catgaccaaa attggggagc tgccggaacc gctggtagta cgaccaatgt
     1441 aatcggtaaa gtacctgtag gaaccgatac ccaaatccaa gacacctcac ttaatattga
     1501 attggaattt ggtgatggct atacgtttac atcgaccaca gggcgtgcag aatatgaata
     1561 cgtagatggt atcgatgcag actttgcagc cattgttctg gtctcaaggg atgactggtc
     1621 gacatatgat caaaccagtc aagaattcca gattgcatcc cctgctgacc gtgatttttc
     1681 attcattgtt ggtacctatt gggacagtca ggagcaagac attgaacgct tgattgatct
     1741 tgatggaacc gtaggtggac tcatgggaat gattggacag ccatcaatct tgaacattcc
     1801 tctagctaac ctgacagcaa tgggactgcc aatgttctat ctaaatggtt caatcaatga
     1861 tccaacggct gcttatctaa tttcacttgg agtcctacca ggaactcctg ctgcttatca
     1921 aacgatcttt gatcatgcca ctcgaatcgg ttactggaat caaaaaactg aagccaaggc
     1981 agtctatttt caaggcactc agcaaatgaa ggaagacctt gcaataactt ttggtgtgcg
     2041 ttacgtagag gagaataaac acatccgtgc tggaacctgt ttgggtgctg atactaccgg
     2101 tctccagacc tgtaacagca atgccttcct agctgcaatc atggctgcca gctttgatac
     2161 atatgctcat aacttcgata atattccaga gcgagaaac
//
LOCUS       DASCTH010000198         7704 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000313659, whole genome shotgun sequence.
ACCESSION   DASCTH010000198 DASCTH010000000
VERSION     DASCTH010000198.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000313659"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="QGH35_04150"
     CDS             <1..493
                     /locus_tag="QGH35_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HJL81760.1"
                     /translation="ENISNIKGKHLIPAFYLNELILSFLKIADPYPDLFKLYTATLHD
                     LSELKSIEPSLRRFEIFLLSEIGYAINFETETLSQKPIEAGLNYVFTAGQGFVSKRDQ
                     NSGSFLIKGLWIKAINEGDFSDPETLRCAKKIVRLSIQYHLDGKELMSKKVFKSIERK
                     SGE"
     gene            789..1772
                     /locus_tag="QGH35_04155"
     CDS             789..1772
                     /locus_tag="QGH35_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HJL81761.1"
                     /translation="MKKFYDHKSPVTGENYRTMLEPRYGGLPTFMRTPLVNTLEKLDI
                     ALVGVPYDGGVTNRPGARHGPREMRSQSSFIREFHHVTRINPFELARIADVGDVRFSK
                     PFDHQIVNQDIENFFRRIHEAGVLPISAGGDHSISYPIFKGIVTDKPIGMVHIDAHTD
                     TWGDIWGSKFHHGSPFRLAVEDGLLDPKRTIQIGIRGGQNFLDGIDFSLTSGMRVVFI
                     EEFTELGVKNIIEEARKVVGEGPTYISFDVDGLDPVYAPGTGTPEVGGITTLEAQQLL
                     RGLDGLDLIGGDVVEVSPPFDHTGNTSLVGVTMMFEILCLLAAQFTDASKP"
     gene            1853..3013
                     /locus_tag="QGH35_04160"
     CDS             1853..3013
                     /locus_tag="QGH35_04160"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HJL81762.1"
                     /translation="MIKCILNPFLSEQREVPHFVGGDQIEAVSFFHQSLPGYAYTPLL
                     KLEGLAAKFKINSLILKDESQRFGLKAFKVLGASYAIAKEIQRLLELKNGRVNFDTIT
                     ANPNELKSLTFITATDGNHGRAVAWCAEQFGCNAVVFMPKGTSKARLEAIQAHGAQAE
                     ITELNYDETVLFSAQQAKENDWVLLQDSSWEGYQTVPKHIMQGYFSLVSEFADQTTEW
                     PTHVIAQAGVGSFAASVFSYFLASPKALPKLILVEPTGAACYFNSIQIGDGKPHLTKE
                     LNTMMAGLACGQPSILAWDIIKSNCDAFVVCEDEIAIKGMQLLANPISNDAVIVSGES
                     GAAPIGFLHEVCINQRHRLIKEELSLDDNSRVLIFSTEGDTDPKTYQEYSKL"
     gene            complement(3036..4550)
                     /locus_tag="QGH35_04165"
     CDS             complement(3036..4550)
                     /locus_tag="QGH35_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJL81763.1"
                     /translation="MIKEHKFGIIIIFLILGSLLSSELASNENSSLLIINAKIIDGTG
                     GAARLESVRINGNRIQELGQLRPLPGEIILDATGLVLSPGFIDTHSHHDISIFNMPDA
                     LSATSQGITTIVVGLDGFSTVPLSNFFAKVKDNPLAVNIASYSGHNSLRNIVIGKDYQ
                     RFSTPKEIEDMKNLLRLDMKAGALGLSTGLEYDPGIYSETDEVVALLEVVPKDQGRYA
                     SHIRSEDVHLESALDEFIEISRKSQLPSQISHIKLARRDLWGQTSWMINKLNAARHEG
                     LDITADIYPYTYYESTMRTLFAKRDFDNRESAEFALTELVHPNQLTLSHYEPDPNLVG
                     KTIAEIAVMRKEDPVTVLMNLIKEDKTKTDSAIGVAMTQPDIDKLMQWEHSNICSDGE
                     LNNGHPRGRGAFARILANYVRSRGILGWETAIHKMTQLSALHMGFKNRGVIKSGAIAD
                     LVLFDPEKIQDNATMDDINRLSSGIINVWVGGELVYSDKKSTGLRPGQVIRRAD"
     gene            complement(4558..5454)
                     /locus_tag="QGH35_04170"
     CDS             complement(4558..5454)
                     /locus_tag="QGH35_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020037451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HJL81764.1"
                     /translation="MAAQSTNHILLIEPAEFFSNLETIDTNHFQKINQEEEKDKIFRK
                     ALDEFRTFEQTLRDNHIHVTTLKGQTGCPDNIFPNWAVTFENKTMHLFSMMAENRRLE
                     KLEDHIDFLNRVYQTTTDLTEFEKQGIFLEGTSSMVLDRVNKVAYMGLSPRSNKKIAC
                     RWAEENDYEIIIFETQGHIGKPIYHTDVLMFIGTDVAVISTNTIKKEYQSTVKNKLSQ
                     MHSIIEITRAQILNFCGNCMEVKGDNNELNLVMSTRAYNSYSGTQKQLLLEHYDKIIH
                     SNITTIENYGGGSARCMMMELF"
     gene            complement(5462..6691)
                     /gene="rocD"
                     /locus_tag="QGH35_04175"
     CDS             complement(5462..6691)
                     /gene="rocD"
                     /locus_tag="QGH35_04175"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HJL81765.1"
                     /translation="MKRSLQKDLIHKELEFGADTYKPLPVVLSKGSGVWVWDVDGNKY
                     LDMMSAYSAVSHGHSHPELIKVLREQSERLVLTSRAFYSETLGAFLEKLTEISGFAVG
                     LPMNTGAEGVETAIKAARRWGYKSKKIPPNKAEIIVADQNFHGRTTTIISFSSDKESK
                     DNFGPLTPGFKTVKFGCIDSLQSAITENTAAVLVEPIQGEAGIVIPDPSWLPKVRKLC
                     TENNVLLILDEVQSGLGRTGKMFAYEHSSIRPDGLILGKALGGGLLPVSCFLSSKEVM
                     QWFVPGSHGSTFGGFSLAAAIGKRSIEVIEEEQLAENSMKLGNYFLNQLKEISSPIIL
                     EVRGKGLWIGVEIDAEIISGRELCKRLLTKGILSKETHKTVIRFAPPLVITKKEIDWA
                     LDIFKQVILEIETEQLN"
     gene            complement(6698..>7704)
                     /locus_tag="QGH35_04180"
     CDS             complement(6698..>7704)
                     /locus_tag="QGH35_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012398.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJL81766.1"
                     /translation="GNRPLIKSATTVGIVFEQFLRLLIHCGLPAIDSDMIHCRGSKMG
                     KLISDANDKIRMVQFTGSCEVAERISEDMNGKIRVEDAGFDWKVIGPDYDPDWSDYVA
                     WQCDEDAYNASGQKCSAQSILFVHKNWENDLLPKLKQLAERRKLEDLSIGPILTWNND
                     QLFDHINSLLEIPGATCLFGGTELKSHTIPKVYGAITATAILVPVDQLLGKDFEVITS
                     EVFGPVQVVVVYENKDLSIIMEALERIPQNLTAAIVSNDVHFQQQVLGNTVNGTTYTG
                     MKARTTGAPQNHWFGPAGDPRSAGIGTSEAIITTWSGHREIIKDIGPLDPKWEIPENL
                     "
BASE COUNT         2255 a         1534 c         1458 g         2457 t
ORIGIN      
        1 tgaaaatata tcgaatatca aaggtaaaca tttgattcct gctttttatt tgaatgaatt
       61 gatcttatca tttcttaaaa ttgcagatcc ttacccagat ctttttaagt tatatacagc
      121 tactctccat gatctttccg aattaaaatc cattgaacct agcttacgaa gatttgagat
      181 ttttctactt tcagaaatag gttatgccat aaattttgaa actgaaactc tttcacaaaa
      241 accaattgag gctggtttaa attatgtttt tactgcagga caaggttttg tctcaaaaag
      301 agatcaaaat tctggttcat ttttaatcaa aggtctttgg ataaaagcaa tcaatgaagg
      361 agatttctct gatcctgaaa ccttaagatg cgccaagaaa atagtgcgtt tatcgatcca
      421 atatcatctt gatggcaaag aattaatgag taagaaagta tttaaatcca ttgaaagaaa
      481 aagtggtgaa tgattaagcc agaagttata gtttacggat tattatggtt ccataatcta
      541 ccctttcaaa attctcacaa cctatccgat ttaatataga aattactaga ttaaccatga
      601 cttggctgtt accgcaaatg atttccaatg gaatttgact ttggtgtagc aaaacaaaat
      661 tttccacgac tctatcaact tggttatgac tgataccgtg gagatcaaga acactattct
      721 tcataatcct tcatacaatt gattataatt aattgatctt aaaataaatt tataatgaat
      781 gaaatgtcat gaaaaaattc tatgatcaca aatctcctgt aacaggggaa aattatcgga
      841 ccatgttgga acctcgatat gggggattgc ctacgttcat gagaactcct ctggtaaata
      901 ctcttgagaa attagacatt gcattggttg gcgttcctta tgatggaggc gttactaatc
      961 gtccaggtgc ccgtcatgga cctcgggaaa tgagaagtca atcaagtttc attcgtgaat
     1021 ttcatcatgt cacccggatc aacccttttg agttagcaag aattgctgat gtcggtgatg
     1081 ttcgattttc caaacctttt gaccatcaaa tcgttaatca ggatatcgag aactttttta
     1141 gaagaatcca tgaagccggt gtattgccta tcagtgcagg aggtgatcat tccattagct
     1201 atccaatttt caaagggatt gtgactgata aacccattgg gatggtacat attgatgcac
     1261 ataccgatac ttggggtgat atatggggct ctaaatttca ccacggttca ccatttcgtt
     1321 tggctgttga agatggttta ttggatccca agcgaaccat ccaaatcggt attcgtggcg
     1381 ggcaaaattt tcttgatgga attgattttt ctttgaccag tgggatgcga gtggtgttca
     1441 tagaggaatt cacagaattg ggggtcaaaa atatcattga agaggccaga aaagtagtgg
     1501 gtgaaggacc aacctatatt tcgttcgatg tggatggtct tgatccagtc tatgcaccgg
     1561 gtacaggaac accagaagtt ggcggaataa caacactgga agcacaacaa ctccttcgag
     1621 gattggatgg tcttgatctc attggtggcg atgtggttga ggtttcccct ccatttgatc
     1681 ataccggaaa cacttctctg gtcggagtca caatgatgtt tgaaatcctg tgtcttcttg
     1741 cagcccaatt taccgacgca agtaagccct gatgatctag aatatcaagc ttttacttct
     1801 cataatttga tacaagccgg atgatatcga tgagattaat agagaggagc caatgataaa
     1861 atgcatttta aatccatttc tctcagaaca aagagaggtg ccccattttg taggtggtga
     1921 tcagatagaa gcggtcagtt tttttcatca aagtttaccc ggttacgcat acacaccctt
     1981 attaaaactg gaaggtcttg ctgcaaaatt taaaattaat tccttaatat taaaggatga
     2041 gagtcagcgt tttgggttaa aggcatttaa ggttttgggg gcaagttacg ccatagccaa
     2101 agaaatacaa cgtttgctgg aattgaaaaa cggaagagtt aactttgata cgattacagc
     2161 aaatccaaat gagctcaaat cattaacatt catcacggct accgatggta atcatggtag
     2221 agcagttgct tggtgtgccg agcaatttgg ttgcaatgct gtagttttta tgcccaaagg
     2281 tacttcgaaa gctcgtttag aggcaatcca agcacatgga gctcaggcag aaatcactga
     2341 acttaattat gatgagacag tcctgttttc tgcccaacaa gcgaaagaga atgattgggt
     2401 attgcttcaa gattcttctt gggaaggata ccagactgta cctaaacata ttatgcaagg
     2461 ttatttcagc ttggtgtcag aatttgcaga tcagacgact gaatggccga cacacgtgat
     2521 tgcccaagca ggagttggtt cctttgctgc atcggtattt tcctatttct tggcaagtcc
     2581 caaagctctg cctaaattaa ttttagtgga accaacagga gcagcttgtt attttaattc
     2641 catacaaatc ggggatggca aaccgcattt aacaaaggaa ctgaatacaa tgatggcagg
     2701 acttgcatgt ggtcagccaa gtattctggc atgggacatc atcaaatcta attgtgatgc
     2761 gtttgtggtt tgtgaggatg agattgcaat aaaaggcatg caattattag caaatccaat
     2821 aagcaatgat gcagttatcg tctcaggcga gagtggggca gctccaattg gatttctcca
     2881 cgaggtttgc ataaatcaaa gacatcgcct aataaaggaa gaactttcac tggatgataa
     2941 ttcaagggta ttgatttttt ccaccgaagg tgacactgat ccaaaaactt atcaagagta
     3001 ttctaagtta taagaaatct tgggtagtaa ttttttcagt cagctctcct gataacctgt
     3061 cccggtctta gacctgttga tttcttatca gagtaaacca attcacctcc tacccaaaca
     3121 ttaatgatac ccgatgataa tctgttgata tcatccattg tggcattgtc ttggatcttt
     3181 tccggatcaa ataagactaa atctgcaatg gcacctgact tgattacacc tcgattttta
     3241 aatcccatat gcagagcaga aagttgagtc atcttgtgaa tggctgtctc ccatcctagg
     3301 attcctcttg agcgtacata atttgctaaa attctagcaa aagcaccccg acctctggga
     3361 tgaccattat tcagttcccc atctgagcat atgttgctat gttcccactg catcaattta
     3421 tctatatctg gttgtgtcat agccacacca attgctgagt ctgtttttgt tttatcttct
     3481 ttaatcaaat tcatcagcac cgtaactgga tcttcttttc tcatcacagc aatttcagct
     3541 atagttttac ccactaaatt tgggtctggt tcgtaatgag aaagtgtcag ctgatttgga
     3601 tgaactaatt cagttagagc aaattcagca ctctccctat tatcgaaatc acgcttggca
     3661 aaaagtgttc tcattgtcga ttcatagtag gtataggggt agatgtctgc tgtgatatcc
     3721 agaccctcgt ggcgagcagc attcagttta ttgatcatcc aagacgtttg accccataaa
     3781 tcccttctcg caagtttgat atgtgatatt tgcgagggaa gttgtgattt tcgactgatc
     3841 tctataaatt catcaagtgc tgattccaaa tgaacatctt cactgcgaat atgactggcg
     3901 taccgtcctt gatccttcgg gacaacttct agcaaggcaa caacctcatc ggtttctgag
     3961 taaatacccg gatcatattc aagtccagtt gacaacccta gcgcaccagc tttcatgtct
     4021 aatcggagaa ggtttttcat gtcttcaatt tcttttggtg tggaaaatct ttgatagtcc
     4081 ttgcctataa ctatattccg aagtgaattg tgcccagagt atgaggcaat attgactgca
     4141 agcggattat ctttcacttt ggcaaagaag ttagagagag gtactgttga aaaaccatcc
     4201 agacccacaa ctatagtagt tatcccttga ctggttgctg aaagagcatc aggcatgttg
     4261 aagattgaaa tatcatgatg gctgtgggta tcaatgaatc ctggggacag aacaagtcct
     4321 gtagcatcta gtattatttc tccaggtaga ggtcttaatt gaccaagttc ttggattcgg
     4381 ttgccattga tcctcacaga ctctaatcga gcagcgcctc ccgttccatc gatgattttt
     4441 gcattgatga tcagtaagga tgagttctca ttggatgcca actcactgct cagtaacgac
     4501 cctaaaatca gaaaaataat aatgattcca aatttatgtt cttttatcat agtcaattta
     4561 gaaaagttcc atcatcatgc atcttgcaga accaccacca tagttttcaa tagttgttat
     4621 atttgagtga atgattttat catagtgctc aaggagtaat tgcttctgtg ttccagaata
     4681 tgaattatat gcacgagttg acatcaccag gttcaattca ttattgtcac ctttaacctc
     4741 catacaattc ccacaaaagt ttagtatctg ggctctcgta atttcaataa ttgaatgcat
     4801 ctgactcaat ttatttttga ctgttgattg atattctttt tttatagtgt tggtagaaat
     4861 aacggccaca tcagtgccaa tgaacatcag cacatcagtg tgataaattg gtttgccaat
     4921 atgtccttgg gtttcaaaaa ttataatctc atagtcattt tcctcagccc atcggcaagc
     4981 tattttttta ttcgatcttg gtgatagccc catataggca actttattga ctctatcaag
     5041 aaccatactg cttgttccct ctaagaatat gccttgtttt tcgaactcag ttaaatcagt
     5101 agttgtttga taaactctat taagaaaatc gatgtgatcc tctaattttt ctagtcttct
     5161 attttcagcc atcatgctga acaaatgcat cgttttattc tcaaaggtca cagcccagtt
     5221 aggaaaaata ttgtcaggac agcctgtttg tcctttgagt gtggttacat ggatatgatt
     5281 atctcttaga gtctgttcaa aagttctaaa ttcgtccaaa gcttttctaa aaattttatc
     5341 cttttcttct tcttgattta ttttttggaa atgattggta tcgattgttt ctagattact
     5401 aaaaaattct gcaggttcaa tgagtagaat atgattggtt gattgtgctg ccatgaatga
     5461 tttaattgag ttgttcagtc tcgatttcca agattacttg cttaaaaata tccaatgccc
     5521 aatctatttc tttttttgta ataaccaacg gaggagcaaa acggataacc gttttgtgag
     5581 tttctttact gaggatccct ttagtcagca aacgtttaca caattcccta ccagaaatta
     5641 tctctgcgtc aatttcaaca ccaatccaaa gacctttacc gcgtacttcc aagataatag
     5701 gactagatat ttccttcaat tgattaagaa aatagtttcc aagtttcata ctgttttctg
     5761 caagttgttc ctcttctatg acttcaatag acctttttcc gatggcagct gctaatgaaa
     5821 aaccaccaaa tgttgagccg tgggatccag gaacaaacca ctgcatgacc tctttgctgc
     5881 taagaaaaca agacactgga agtagaccac cacccaatgc ttttcctaaa atcaatccat
     5941 caggccttat cgaagaatgt tcataggcaa acatttttcc tgttcttcca agacctgatt
     6001 ggacttcgtc taagattaga agcacattat tttccgtaca gagcttacga acttttggta
     6061 accaagatgg atctggaatc acaattccag cttcaccttg aatgggctct acaagtacag
     6121 ctgcagtatt ctcagtaatc gcagattgaa gtgaatcaat gcaaccaaat ttcactgtct
     6181 taaatcctgg ggtaagggga ccaaaattat ctttggattc tttgtctgag gagaaactga
     6241 tgattgtggt tgttcttcca tgaaaatttt gatcagcaac tataatctca gccttgttag
     6301 gaggtatttt tttgctttta tagccccatc ttctagcagc tttaatggct gtttccaccc
     6361 cttccgcacc tgtgttcatc ggtaaaccaa ctgcgaaacc tgaaatctct gtcaattttt
     6421 ccaaaaatgc tccaagagtc tcggaataaa aagctctaga ggttagtact aagcgttcag
     6481 attgttcccg taaaacttta atcagctcgg ggtgagaatg accgtgactc accgctgaat
     6541 aagcggacat catatcaagg tatttatttc catccacatc ccaaacccat acacctgagc
     6601 ctttggacaa caccacaggg agaggtttat aagtatctgc accgaactct aattctttat
     6661 gaatcaaatc tttctggagg gatctcttca tgtcattcta taaattttca ggtatctccc
     6721 atttaggatc aagtggaccg atatctttaa taatctccct atgcccactc caagtagtta
     6781 tgatggcttc agaagtacct atccctgctg atctcgggtc tcctgctggt ccaaaccaat
     6841 gattttgagg agcaccagtt gttctcgctt tcatccccgt ataagtggtt ccattgacag
     6901 tattgccaag aacctgttgt tgaaaatgaa catcattact tacaattgca gctgtgagat
     6961 tttgtgggat tctctccaaa gcttccataa tgatagatag atccttattt tcatatacca
     7021 ctactacctg aactggtccg aaaacttcac tagtaattac ttcaaaatcc ttacctagga
     7081 gttgatccac aggtacaaga attgcagttg cagtaatcgc accgtacact tttgggatcg
     7141 tgtgagattt aagttcagtt ccaccaaata aacaagtggc accaggtatt tctagaagac
     7201 tattgatgtg atcaaataat tgatcattat tccacgttaa tataggacca atactgaggt
     7261 cttctaattt tcttcgttct gccaattgtt ttaattttgg caggaggtcg ttttcccagt
     7321 ttttatgaac aaataatata ctttgggctg aacatttttg tccggatgcg ttgtaagcgt
     7381 cttcgtcaca ttgccaggct acataatcac tccaatctgg atcgtaatcg ggcccgataa
     7441 ctttccaatc aaatcctgca tcttctactc tgatttttcc attcatgtcc tcagaaatcc
     7501 tttcagccac ttcacaagag ccagtgaatt gaaccattct tattttatcg ttagcgtcag
     7561 aaatcaattt acccattttt gaaccccgac aatgaatcat gtcagagtca attgcaggaa
     7621 ggccacagtg aattaatagt cttagaaatt gctcaaaaac aatgccaacc gttgttgcag
     7681 atttgattaa cggtcgattt ccca
//
LOCUS       DASCTH010000199         4061 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000316477, whole genome shotgun sequence.
ACCESSION   DASCTH010000199 DASCTH010000000
VERSION     DASCTH010000199.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000316477"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(132..1682)
                     /locus_tag="QGH35_04185"
     CDS             complement(132..1682)
                     /locus_tag="QGH35_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HJL81767.1"
                     /translation="MKQIGRPLIALILFLVPVEATISEVNVSINLHNSQIEGVFNSDY
                     ELYIFRGIPYAKPPVGDLRWQSPVSLSSDPEVINAQSFKPACMQDEYNTEWYQDVAEL
                     FDNPRSVFQHVDEVSEDCLYLNIWTNSLDQTSKKPVMVWVHGGADTGGWSYEPNYIGH
                     HLASKNVVVVSINYRLNIFGFFNHPEMSNQTGNFGLEDEIMALQWIKDHISFYGGDPE
                     NITYFGESAGGAHVSYLITSPKARGLFKRGIIQSGGYNLFRWTSKDRAQKLGVRAQTV
                     LNAPDLETMKTLPAESLLSVSTSLGHPYRPSIDGTLIPNNVAQLLEAGSFNNVDLMIG
                     SNKNEDYMYVDKAITENNINRLIDYYYPEHQDQLLSLLDLEDPRLAMDHLTTNQQTLC
                     PSIFIARSLAKNGNNVYQYHFTRMREGSEKILSYHGAEIPYVFNTHDEWLPTNEDDAQ
                     LTEAMMEYWIEFAKKGTPNSINNPTWKEFGTEENYLILDLPLLNSSKLENEFCEIMIN
                     ELVQRANY"
     gene            complement(1690..2670)
                     /locus_tag="QGH35_04190"
     CDS             complement(1690..2670)
                     /locus_tag="QGH35_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HJL81768.1"
                     /translation="MKAVWYESIGEAEQVLNYGDLEDPQINDGEVLVKLKTSGVNPSD
                     VKIRAGARGELQFPRIIPHSDGGGIIVKVGEEVSEDRIGERVWIWNGAFGRAFGTCSE
                     LIVLPSSQAVTMPDDVSFETAACLGIPASTAYYGIFADGPVKDQTILITGGAGAVGYL
                     GIQLAKWSGAHVISTVSGDDKAAAAEAAGADLVVNYKTDDVIKAVNDFTKGHGVDRIL
                     EVEFGGNLSVSEHVIKNNGVIAAYGSVAEANPSVPFYNLMFKGVKLNTYLIYIVADSD
                     RANINQGINAALSDNVLTPIIAKSFQLSETVNAHQSVEEGSVIGNVVITI"
     gene            complement(2667..3473)
                     /locus_tag="QGH35_04195"
     CDS             complement(2667..3473)
                     /locus_tag="QGH35_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006012850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81769.1"
                     /translation="MTKEISSMRIAITAAASGIGSYIAAELNALGAQIAICDHDKERL
                     EAFKENHDNILVTKADVAIESEVIDFFRDINSNLGGLDALINNAGISGPIANLEDVKF
                     SDWENTLSVNLNSAFLCSKEAVPMLRNSGGGSIINIGSSSSFFGTPLRSAYSASKWAL
                     IGLTKTWAMELGQDNIRVNAICPGSVNGPRIEKVIEQEASERNTDPSMIKEAYLNQTS
                     LRTFIDPEEITGMVKYLLSPIATKVSGQAISIDGHTESLSQIRTKEDNNE"
     gene            complement(3487..3951)
                     /locus_tag="QGH35_04200"
     CDS             complement(3487..3951)
                     /locus_tag="QGH35_04200"
                     /inference="COORDINATES: protein motif:HMM:NF025268.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HJL81770.1"
                     /translation="MKKIILIASLLLCSNGWSGDEYLPYDETADAQTELNTAINTAKE
                     KNKHVLIEMGANWCPDCRTLGRYFQREDIKVWLDERFIVVAVDIGQWDKNLEIVEKYG
                     NPISEGIPSLVVLNANGKMQFATLAGELATARNMSGDDLITWLKEKIQPVID"
BASE COUNT         1115 a          910 c          757 g         1279 t
ORIGIN      
        1 tctggctgga tcaagtattg cctatgtata gaaaagacac atacagaata ataggcgtaa
       61 taggaatggt taaatcaacc aatcatcgtt tcagagtgat cactattttt gaaaggctat
      121 aaagcactga attagtaatt tgctctttgc actagctcat tgatcatgat ttcacaaaac
      181 tcattttcca gctttgatga attcagcaat ggcagatcta atatcaagta attttcttca
      241 gtcccaaact ctttccatgt tggattattg atggaattgg gtgttccctt ttttgcaaat
      301 tcaatccagt attccatcat cgcttcagtc agttgtgcat catcttcatt agttggcagc
      361 cattcatcat gggtattaaa aacatagggt atttccgcgc catgataaga aagtatcttc
      421 tcactgcctt ctcgcattcg agtaaaatga tattgataga cgttatttcc gtttttcgca
      481 agagacctgg caatgaaaat tgaaggacaa agtgtttgtt gatttgttgt cagatgatcc
      541 atggcaagtc ttggatcctc tagatccagc aatgagagta attggtcttg atgctctggg
      601 tagtagtaat caataagccg attaatatta ttctctgtga tggctttatc aacatacatg
      661 taatcttcat ttttattgct gccaatcatc agatcaacat tattgaatga ccccgcctca
      721 agtaattgag ctacattgtt cggtatcaat gtaccatcga tgcttggtct atagggatga
      781 ccaagactgg ttgagactga taatagactt tcagctggta aagtcttcat agtctctaaa
      841 tcaggggcat tcaagacagt ttgtgctcta acacctagct tctgtgccct atcttttgat
      901 gtccacctga aaagattata accaccgctt tggatgatgc ctcttttaaa taaaccccta
      961 gcttttggtg aggtaattaa ataggagaca tgagccccac ctgccgattc tccaaagtag
     1021 gtaatatttt caggatcacc cccataaaat gagatatgat ctttgatcca ttgcagagcc
     1081 ataatttcat cttcaagacc aaaattccca gtttgattcg acatttcagg gtggttaaaa
     1141 aaaccaaaaa tattcaatct ataattgata gagaccacta ctacgttttt gctggcaaga
     1201 tgatgaccta tataattggg ctcataggac catccccccg tgtccgcccc tccatgcacc
     1261 caaaccatga cgggtttctt actagtttgg tcaagggaat ttgtccatat attgagatag
     1321 agacaatctt cacttacttc atcaacatgt tgaaaaacag agcgaggatt atcaaacaac
     1381 tctgcgacat cttggtacca ttcggtgttg tattcatctt gcatgcaggc cggtttaaag
     1441 ctttgggcat tgattacttc tggatctgac gataaagaaa cgggtgattg ccaccgtaga
     1501 tctcctaccg ggggcttggc atagggaatc cctctgaaga tatacagttc atagtcactg
     1561 ttaaacaccc cttctatttg tgaattgtgc agattaattg aaacattcac ctcagatatt
     1621 gttgcttcca cggggacaag aaagagaatt agagcgataa ggggtcgacc tatttgtttc
     1681 attatttact caaatagtga tcacaacgtt gccgataaca gaaccttctt ccactgattg
     1741 atgggcatta acagtctcag acaactggaa ggatttagca atgatgggag tgagtacatt
     1801 atcactaagg gcagcattaa ttccttgatt gatattagct ctatcactgt cagcaacgat
     1861 atagatgaga taggtattta atttcacacc tttgaacatc aagttataga aaggcacaga
     1921 ggggttagct tcagcaacag agccataagc cgcaatcaca ccattgtttt ttatcacatg
     1981 ctcactgacg gataaatttc caccgaattc aacctcgagt attctgtcaa ccccatggcc
     2041 ttttgtgaaa tcattgaccg ccttaataac atcatctgtt ttatagttca ccactagatc
     2101 tgccccagca gcttcagctg cggctgcttt gtcatcaccg ctgactgtag atataacatg
     2161 tgctcctgac cattttgcaa gttgtatgcc cagataacct acagctccgg cacctccagt
     2221 tattaatata gtttgatctt ttactggacc atcagcaaag ataccgtaat aggcagttga
     2281 tgctggtata cctaagcaag cagcggtttc aaatgaaaca tcatcaggca tagtaaccgc
     2341 ttgtgaggag ggcagcacga tcagttctga acaggtccca aaagctctac caaaagcacc
     2401 gttccagatc catactcttt cgccaatcct atcttcactg acctcttcac cgactttcac
     2461 aatgataccg ccgccatctg aatgagggat gattctggga aattgtaatt ctcctcttgc
     2521 cccagctcta atctttacat cagaaggatt gaccccggat gttttcaatt tgaccaatac
     2581 ttcaccatca ttaatctggg ggtcttccaa gtctccataa tttaggactt gctctgcttc
     2641 cccaatagat tcataccaaa ctgctttcac tcgttattgt cctctttggt tctaatttgg
     2701 cttaagcttt ctgtgtgtcc gtctattgat attgcttgtc ctgagacctt ggttgctatg
     2761 ggtgacaata gatactttac catcccagta atttcttctg gatcaatgaa ggtccgcagt
     2821 gaggtttggt ttaaataggc ttccttgatc atagacggat ccgtatttct ttcgctggcc
     2881 tcttgttcga ttactttttc aattctcgga ccattaaccg atccggggca aattgcattg
     2941 acacggatgt tgtcttgacc caattccatt gcccatgttt ttgtcaatcc tattaatgcc
     3001 cactttgatg ccgaataagc agatcgtaat ggggtgccaa agaaggatga acttgatccg
     3061 atattgatga tcgaacctcc acctgagttt cgaagcatgg gtacggcttc tttgctacac
     3121 agaaacgcac tgttgagatt gactgataag gtattctccc aatcagaaaa ctttacatct
     3181 tcgagattgg ctatggggcc tgatataccg gcgttattga tcaatgcatc aaggcctccg
     3241 aggttgctat tgatgtctct gaaaaaatca atcacctcac tctcaatagc tacatctgct
     3301 ttagttacca atatattatc gtggttttct ttaaaagctt ccaaacgctc tttgtcgtga
     3361 tcacaaattg caatttgtgc acccaatgca ttaagttctg ctgctatata agagccaatt
     3421 ccacttgcag ctgcagtgat tgcaatcctc attgatgaaa tttctttggt catatacttg
     3481 attgcatcaa tcaatcacgg gttgaatctt ttcctttagc caagtaatga gatcatcccc
     3541 actcatattt cttgcagtgg caagctcacc ggcaagcgtt gcaaattgca tcttgccatt
     3601 ggcgttcagg accaccaatg aaggaatacc ttcactaatg gggttgccat atttttcaac
     3661 aatttccaaa ttcttatccc actggccaat atcaacagca acaacaataa agcgttcgtc
     3721 caaccacact ttgatgtctt ctcgttgaaa atatctacct aaagtcctgc agtcagggca
     3781 ccagttggct cccatttcga tcagcacatg tttgttcttc tccttggcag tgttgattgc
     3841 agtgttcaac tcagtttggg cgtctgcagt ttcatcatag ggcagatatt catccccact
     3901 ccacccatta gagcagagta gtaggctagc tattaatata attttcttca tacttctatt
     3961 agtttacaag acttgcttag ttatgcttac ccttattcca cccattttta gttaaatatt
     4021 tttcagccga ttctattgcc ccttcctcaa tacttgtacc c
//
LOCUS       DASCTH010000200        18176 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000319826, whole genome shotgun sequence.
ACCESSION   DASCTH010000200 DASCTH010000000
VERSION     DASCTH010000200.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 18176)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18176)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18176
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000319826"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="QGH35_04205"
     CDS             complement(<1..523)
                     /locus_tag="QGH35_04205"
                     /inference="COORDINATES: protein motif:HMM:NF013714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HJL81771.1"
                     /translation="MLKYIAQQFEHDCSYDEPMAGHTSWGIGGPVDLFFSPKNRDELV
                     SFLSQIDSRIPITWIGKGTNMLVRDRGVRGVIISTKSFLQDITKISHQNYTVEAGVSC
                     MDLALFCQKINIGPAAFFSGIPGSIGGALVMNAGAFGHETWNQVIKVEVIDQKGRIQY
                     LDSDQFRVSYRTVN"
     gene            complement(553..1704)
                     /gene="murG"
                     /locus_tag="QGH35_04210"
     CDS             complement(553..1704)
                     /gene="murG"
                     /locus_tag="QGH35_04210"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HJL81772.1"
                     /translation="MNQNTTYSMIIAAGGTGGHIFPALAIAKHFKANGHRVIIVGTGN
                     NLERKIFGQNDIEVKYFKARKIKRRGLMTFFDPMNYQNIYSTFFKPDAELVKFLKDFE
                     PDLVLGMGGYPSFSICSAFNRSDKTVVVIHEQNSKAGLANRYLTLSVAYAVIEGLPGG
                     IGRIIKFFVDDCIFLGNPVRQEIIDTRTLPREFPDNSKKPRILIVGGSQGAHSINNAV
                     PKAMHLLSEKIDLEIRHDTGKADYEEVKKIYEELNINATVSEFINDISEAYGWADLVI
                     ARAGALTVSELSAVGLPSILIPYPQATDNHQFYNAKFLVDKKAAEMILDQDLDPDKLA
                     VTVESFLLEKDKLKNASIAAHDETFALATEKISDYCINIMKKRTYGSSQ"
     gene            complement(1704..2864)
                     /gene="ftsW"
                     /locus_tag="QGH35_04215"
     CDS             complement(1704..2864)
                     /gene="ftsW"
                     /locus_tag="QGH35_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HJL81773.1"
                     /translation="MNQPFSKKKRFSLFENIDNLFVVSVVLLLMGGMVMMTSASMPIG
                     VRDFADPFHYFFKQGFAACLGLIVGFLFFLIPTNFWEKLGILTALIAIGLLALVYIPG
                     IGLEANGATRWVDIGPLPTIQVIDPARFFLIIYIAGYCFRQQKQVEMTFLSLIKLMIF
                     ILPACILLLLQPDFGATVVLLAIVASLFFVAGFKFKYFFVLLAALASVFWLLVYKVQY
                     RLDRVTSFLDPWADPLGDSYQLVNSLIAIGSGEWFGVGLGESVQKLGYLPEAHTDFIF
                     AVIAEELGLIGVVLMFGIYTLMIWRIFSISLHAMKHNRVFQAYFAFSVGIWITSQVVV
                     NIGVNMGVLPTKGLILPLISYGSNAMTMTLITLFIVLKIGCENPVIKTNRKT"
     gene            complement(2893..3972)
                     /gene="mraY"
                     /locus_tag="QGH35_04220"
     CDS             complement(2893..3972)
                     /gene="mraY"
                     /locus_tag="QGH35_04220"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HJL81774.1"
                     /translation="MFLLFSNDLIELLSAFNALTYITTRGILSALTALTVSFLIGPVL
                     IRKLSFNEIGESIRADGPKSHAVKQGTPTMGGVLILFSITFSTVIWGDLTNRYVINAL
                     ILMNLFGLIGFLDDYMKLNKIKNGLQASIKILMQLFLALISVLVLYYYTQSPIETELV
                     IPYTNNLTIQMGIFFIPFAILVIVGSSNAVNLTDGLDGLAIMPTVMIAAALGIFAYVA
                     GNINFSDYLGVPYIKDAGEVFIFCAAIAGSGLGFLWFNTYPAQVFMGDVGALSLGAAL
                     GLVSVIVRQELVLVIMGGIFVLETVSVMLQVVSFKLTGKRMFNMAPIHHHFELKGWAE
                     PKVIVRFWIITVMLVLVGLTSLKLR"
     gene            complement(4053..5735)
                     /locus_tag="QGH35_04225"
     CDS             complement(4053..5735)
                     /locus_tag="QGH35_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013100.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HJL81775.1"
                     /translation="MKTKTKEKKPHNNPFRKRSFIIFALIISAGCLLIVSSFRLQYLE
                     REFYNQKADNRHIKEEKIVSNRGSIFDRRGTPLAVSTPVDSVVVNPLEYLKNKEKLFL
                     LSRILSSDSKEILKKIEKKSKKSFMYLKRHISQNQSKEIKKLGDIKGLWLEKEYKRYF
                     PAGEVTGHLIGFTNIDDRGQEGMELLLQDFLVGENGSKLVLKDRFGTLISDIQLIKNS
                     VDGEDFYSSIDLRIQYLAHRALQAGITEFRAKAGSAVVLDVLTGEVLAMVNQPSADPN
                     IRSLRKPKFFKNRSVTDQLEPGSTFKTLTVATALENSIYTSASTVDTTPFKIGTKLIG
                     DDRCQGELDLEMILVRSCNAGAAKISLAMKQEIFCDTLTKLGIGEITASGFPGEARGE
                     LGCYENWRSIKQATLSYGYGVSVTSLQLAKAYSAIANKGIVNRISIEKVKEKPNGERA
                     LSESTTANVLMMLEAVVEHSVSRAKVKGYRVGGKTGTAQTFSDDYSGNAHNAFFVGIA
                     PISNPRIVTVVVVNEPQGQKYFGGQVAAPIFSSIVSGALRIMGIAPDSNTQS"
     gene            complement(5719..6012)
                     /gene="ftsL"
                     /locus_tag="QGH35_04230"
     CDS             complement(5719..6012)
                     /gene="ftsL"
                     /locus_tag="QGH35_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016860.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HJL81776.1"
                     /translation="MNVDNKWYIRAFVLFTLVCISSVGIVYLEHQKRELFSDYQNLIK
                     ERDAIQVEWKKLQIQSSEASSGLRLENIARSKASMKTLDADVINIIEVNENQN"
     gene            complement(6009..6935)
                     /gene="rsmH"
                     /locus_tag="QGH35_04235"
     CDS             complement(6009..6935)
                     /gene="rsmH"
                     /locus_tag="QGH35_04235"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HJL81777.1"
                     /translation="MHHPVLVDKVVQHLIQDVSGIYLDVTCGFGGHTEAILNKLNSKG
                     RVIAMDQDQDAINFTKNKFVNEDRVTVVKGKFGDLRQTLGLHDLDFQWSGILADLGVS
                     SFQLDNAERGFSFQKEGPLDMRMDQSEGTNTQIWINAASENEISQVLWRFGEENYSRK
                     IAKAIINYRIDNQITTTKQLSEIVMEAKQREKIKSRKHLATKTFQAIRIYINQELDQL
                     KQLLDFSLHGLKIGGRLCIISFHSLEDRIVKRFFRNQSRVDPNLLKLPNLTEQDLPKL
                     KIITKRIKSSNEEIQFNPRSRSAILRVVERIR"
     gene            complement(6988..8130)
                     /gene="ftsZ"
                     /locus_tag="QGH35_04240"
     CDS             complement(6988..8130)
                     /gene="ftsZ"
                     /locus_tag="QGH35_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HJL81778.1"
                     /translation="MPEFAYQLMDTDKQKANIKVIGIGGGGGNAVSHMALQGIQGVDF
                     LCANTDTQALAATGVENKLQIGINETKGLGSGSKPEKGRASAQEDRERIASAIKGTGE
                     EKTNMLFIAAGLGGGTGTGASPVVAEIAQELEILTVAVVTTPFSWEGLTRVECAEKGI
                     EDLRKCVDALILIPNDKLQSLGKATTMIDAFGAANDVLLNSVQGIAELITIPGLINLD
                     FADVETVMTASGRGIMGVGSASGHDRGRDAVERAIQSPLLADINLKGARGILLNITGG
                     SDLTVGEFEEIGKIVEGYASENAITVVGTALDPSMQDSIKVTVVATGLDPKTENRSEI
                     ITDNKIDILDVPITKRNKDLFEDLYSEEEEEIDILDVPSFLRRTRA"
     gene            complement(8153..8608)
                     /gene="mraZ"
                     /locus_tag="QGH35_04245"
     CDS             complement(8153..8608)
                     /gene="mraZ"
                     /locus_tag="QGH35_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005769445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HJL81779.1"
                     /translation="MFRGLNKICLDTKGRITLPVRYKESLSLLSSNLLICTIDQDHCL
                     LLYPFKYWEQIEKQIMELPTLNPTSRRLQRLMVGHAAELEMDSNGRVLIPKELRNFAK
                     LRKQVMLVGQGKKIELWKYEDWNKGREKWLSTKDVDLSNLPSELGTLSL"
     gene            complement(8746..9084)
                     /gene="rnpB"
                     /locus_tag="QGH35_04250"
     ncRNA           complement(8746..9084)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="QGH35_04250"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(9123..9959)
                     /gene="rsmI"
                     /locus_tag="QGH35_04255"
     CDS             complement(9123..9959)
                     /gene="rsmI"
                     /locus_tag="QGH35_04255"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HJL81780.1"
                     /translation="MSGLYLVSTPIGNLQDITLRAIETLKIADLVFAEDTRVTRKLLS
                     HLSIKKKIHLLNEHNEKKVAERILTELSDGKTVALVADAGTPLISDPGFRLVKKTRES
                     SFDVIPIPGCCAAIAALTASGMATNRFYFHGFLPKAESEKSKELKQLALRQETMIFYE
                     SVHRLQATITTMSQIFGEDRKGVVCKEITKLHEHFIGNTLAEIDAFISDNPTNIKGEF
                     VLIIDGVKPQKGSIDFDQVDKILMELLSEMSIKKAVKICRIITGYSRNDIYHRALEIK
                     PY"
     gene            complement(9977..10609)
                     /locus_tag="QGH35_04260"
     CDS             complement(9977..10609)
                     /locus_tag="QGH35_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HJL81781.1"
                     /translation="MTSVSNRRSMMELYSGNTCIRSHQVRFVLSEKGISTEINHNEGN
                     AISEDLMTLNPYASLPTLVDRELVVYESGVIIEYLDERYPHPPLMPVSPVDRAKLRLA
                     LKTLEKDIVEPAIELDLAHGTKAENAQRKKLKSILASSSDLFNLQRFFLSDDFTVVDC
                     VIAPVLWRLDLFGIDPSNYNEAMNKYMELVFSRPGFKNSLTEDEAEMRTD"
     gene            complement(10662..11336)
                     /locus_tag="QGH35_04265"
     CDS             complement(10662..11336)
                     /locus_tag="QGH35_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014247.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HJL81782.1"
                     /translation="MNIAKTTIVTCLYVLFIQSELIVASDDVSTTNVQIANNDVSDTA
                     SLQRGARNFFNHCAGCHSLKYMRYNQMAEDLDIEENQLIENLMFTQAATQDPITTSML
                     PSDGDRWFGKSPPDLSLIIRSRSADYVYNFLRGFTIDKYSPTGTDNQVLPGTSMPNIL
                     WLVKEQLSHEEFDQFALDIVNFLDYVAEPIQLKRKNIGVWVILFLLIFLIFSYLLYKD
                     IWKEVH"
     gene            complement(11333..12586)
                     /locus_tag="QGH35_04270"
     CDS             complement(11333..12586)
                     /locus_tag="QGH35_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009725785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HJL81783.1"
                     /translation="MADNQKKDQSIFTNTLLTWIDERFPLSSMIRDHATEYYASKNFN
                     IWYIFGVLSTVVLVIQLFTGLFLTMSYKPSVQEAFDSVEYIMRSVEWGWLIRYMHSTG
                     SSFFFIVVYLHMFRAMLYGSYKKPRELIWIIGMLIYFCLMAEAFFGYLLPWGNMSYWA
                     AGVIVNLFTAIPVIGETLAEWIRGDYYISDITLNRFFAFHVIAIPIALIALVVAHILA
                     LHHVGSNNPDGIEIKDYKDEEGKPVDGVAFHPYHTSKDLVGITIFLIFYCAAIFFAPE
                     MGGLFLEVDNFEPANPLQTPEHIVPAWYYTPYYAILRAVPNKLMGAIILVAAVLCFVL
                     LPWLDKCKVKSIRYRGWQYKVGLYLFAISFLVLGYLGMKPSTGIYVILARLFTIIYFA
                     FFLLMPFYTRFEKTKPVPNRVIYKK"
     gene            complement(12579..13190)
                     /gene="petA"
                     /locus_tag="QGH35_04275"
     CDS             complement(12579..13190)
                     /gene="petA"
                     /locus_tag="QGH35_04275"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HJL81784.1"
                     /translation="MDQEDKDIKTNSNRRKFLSIATSVTSVVGILFATIPFISSFQPN
                     KKAKALGAAIKIDVSKLEAGSILKVIWRGQPIWVLRRSEGMLKTLQKKNLNLTDPESE
                     MSVQPNFAQNKFRSLKPEFFIVEGVCTHLGCAPIEKLILPSVDMGSDWEGGFYCPCHG
                     SKFDLSGRVFSGFPAPANLRVPPHRYQDENTVIIGESEEIVNG"
     gene            complement(13251..14510)
                     /gene="murA"
                     /locus_tag="QGH35_04280"
     CDS             complement(13251..14510)
                     /gene="murA"
                     /locus_tag="QGH35_04280"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007643210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HJL81785.1"
                     /translation="MDKFKIQGGQTLSGEINISGSKNAALPLIAASIMFDERIVLDKI
                     PHLRDVSTMIRLLAGMGVSINLIDSNKLELDASNINNYLAPYNLVKTMRASILVLGPL
                     LAKYGEARVSLPGGCAIGARPVNLHLDGLKAMGAEIKLDGGYIFAKAKKLKGVHFEFN
                     PVSVTGTANLMMAGVLAEGKTILVNAALEPEVISLGMFLKKTGAKIKGLGTKKIEIEG
                     VEKLHSAVYENIPDRVETGTYLVAAILTKGNLILRETQPQHLMSVINLLKQTGAKIFV
                     NNNDIEVSMYQEKIKPIDFVTEPYPGLPTDMQAQLMVLNTVASGSSTVLETIFENRFM
                     HVLELQRMGADISLSRNKAYITGVEKLLPAPVMASDLRASAALVLAGIVAEGETVIDR
                     IYHVDRGYEHIENKLTRVGAKITRIEA"
     gene            complement(14540..14770)
                     /locus_tag="QGH35_04285"
     CDS             complement(14540..14770)
                     /locus_tag="QGH35_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013851.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HJL81786.1"
                     /translation="MNSDDIKILIENSIEDSVALVSSNDNVHFDAIVISDAFNEKSLI
                     ERHQMVYECLGKRMNSEIHALTIKALTPAENS"
     gene            14917..15897
                     /locus_tag="QGH35_04290"
     CDS             14917..15897
                     /locus_tag="QGH35_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HJL81787.1"
                     /translation="MIKKQEKFNFIATGKKVLAIESDAIKVLKDRLDSSFAAACEACF
                     ECTGKIIVMGIGKSGHIADKLAATLASTGTPSFFVHPGEAGHGDLGMISNNDIVIALS
                     NSGETEEIIDLLPALRSMEIKIIALTGNKNSKIANYSNIHIDVRVEEEACPLNLAPTA
                     STTAALAMGDAIAVALLEKRGFSIEDFAKSHPGGSIGKRILLLVEDIMHTNDEIPIVF
                     ENETLAQGLIEMSQKALGMTTVVNKEGDLVGIFTDGDLRRKLEENVDIQKTLMHEVMT
                     KDCLTAAPNTLAVKAIEMIQANKITSLAVTKGTKVIGALNIHDLFRAGVM"
     gene            15898..16428
                     /locus_tag="QGH35_04295"
     CDS             15898..16428
                     /locus_tag="QGH35_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HJL81788.1"
                     /translation="MAPLQSFSKELVSIAKEIKLIALDVDGVLTDGSLYFDDDGNVEI
                     SFNVKDGYGIVSLRKKYGIETIIISGRSSNALLHRIAELNISRFWVNQPNKLEAINII
                     IQEGVIKPNQILYVGDDIPDLEVIENVGLFVAVQDAHEMVKEKAHWITNASGGQGAVR
                     EVCDLVHYSIENNDYD"
     gene            16421..17017
                     /gene="lptC"
                     /locus_tag="QGH35_04300"
     CDS             16421..17017
                     /gene="lptC"
                     /locus_tag="QGH35_04300"
                     /inference="COORDINATES: protein motif:HMM:NF018537.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HJL81789.1"
                     /translation="MTNYLTKLVILALLAIGSSFIYFFNFYSDEDLETQDIISTDYTI
                     KNAKLFGTNTEGKFLYKIIAKEAKVNRTNQQIYLTGVNIKYDTHQNIDWVITADKGQV
                     LSSSNVLVLNGNVILENLIKKSNEAMPYLLLPELNVAFTNAILEKTKLTTEYLEVNPS
                     TYTITTNRNVLIKKDDITIQAQGLTAHIKENELKWERL"
     gene            17014..17739
                     /gene="lptB"
                     /locus_tag="QGH35_04305"
     CDS             17014..17739
                     /gene="lptB"
                     /locus_tag="QGH35_04305"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HJL81790.1"
                     /translation="MSILVVEDLEKNYNTKKVVKNISVNVKSGEVVGLLGPNGAGKTT
                     AFYMIVGLISCRRGRVSIDEYNLTNLPMHHRAKIGLSYLPQEASIFRKLTVEQNIVAV
                     LQAQPGLKTIDIEEKLEDLLTQMNINDLRTSLGISLSGGERRRVEIARALATTPKFIL
                     LDEPFAGVDPISVIDIQRTIRDLAQQDIGILITDHNVRETLGICTRAYIMNQGSVITS
                     GTPQQILENKDVKKFYLGDNFRM"
     gene            17810..>18176
                     /locus_tag="QGH35_04310"
     CDS             17810..>18176
                     /locus_tag="QGH35_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015413.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HJL81791.1"
                     /translation="MQQPDNNLGQLIQDLESDQVAGIRKTLTSLNPPEIARLLESLTV
                     GKRKIIWQLVDQEDEGEILKELVDDVRQNLIEEMDASELIAVTQDMELDDLADLLIDI
                     PEAITEQIITALDQQDQVRL"
BASE COUNT         5855 a         3636 c         2994 g         5691 t
ORIGIN      
        1 aattaactgt tcgataactg accctgaatt gatcgctatc taagtactga attcttccct
       61 tttgatctat cacctcaacc ttaataactt gattccaggt ttcatgacca aaggctcctg
      121 cattcattac taaagcccct ccaattgaac cgggtatccc agaaaagaac gcggctggtc
      181 ctatattaat tttctgacaa aatagagcta gatccataca agagacacca gcttctacag
      241 tgtaattttg gtgtgatatt ttcgtgatgt cttgcagaaa ggatttcgtg ctaataataa
      301 cacctcgaac acccctatct ctaacaagca tgttggtacc tttaccaatc catgtaatgg
      361 gtattctaga atctatttgt gaaagaaatg aaactagttc atctctattt tttggagaaa
      421 agaagagatc aacaggtccc ccaattcccc aagaggtatg tcccgccatg ggttcatcat
      481 atgagcagtc atgttcaaat tgttgtgcaa tatactttaa caaatttaaa ggtctccaat
      541 tagagtggcc attcactgag aagaaccata ggtccttttt ttcattatat ttatacaata
      601 atctgaaatt ttttctgtcg ccagtgcaaa agtttcatca tgagcagcaa tcgatgcgtt
      661 ttttaatttg tctttttcta aaagaaaact ttctacagtg acagcaagct tatctgggtc
      721 aagatcttga tctaagatca tttctgctgc ttttttatca accaaaaatt ttgcattata
      781 aaattgatgg ttatcagttg cttggggata tggtatcaat atcgatggta aacctactgc
      841 tgagagctct gagactgtta aagcgcctgc cctagcgatg accaagtctg cccacccata
      901 agcttctgag atgtcgttta taaattctga aacagtggca ttaatattta attcctcata
      961 aatcttcttt acctcttcgt aatcagcttt tccagtatca tgtcggatct caaggtcgat
     1021 tttttcactc aataaatgca ttgctttagg gactgcatta tttatagagt gtgcaccctg
     1081 actcccacct acaataagaa ttcttggttt ttttgagtta tctggaaatt ctctaggtag
     1141 cgttcttgtg tctatgattt cttgtcgaac tgggtttcct aaaaatatac aatcatcaac
     1201 aaagaatttt attattctac cgattcctcc cggcaatcct tctataacag catatgcgac
     1261 cgacaatgta agatatcgat ttgcaagacc tgcctttgaa ttttgctcat gaatgactac
     1321 aacagtctta tcagatcgat tgaaagcgct acatatgcta aaggatggat aaccccccat
     1381 acctaaaacc agatcaggct caaaatcttt taagaattta actagttcgg catcaggttt
     1441 aaagaatgtt gaataaatgt tctggtaatt cattggatca aagaaagtca ttagacccct
     1501 acgctttatt tttctagctt taaaatactt aacttcaata tcgttttgac caaaaatttt
     1561 cctttctaaa ttattacctg ttcccacaat aatgaccctg tgaccgtttg ctttaaaatg
     1621 tttagcaatc gctaaggcag gaaaaatgtg gccaccagtt cctcctgcag cgatgatcat
     1681 tgaataagtt gtattctgat tcatcaagtt tttctattgg tcttgataac tggattctca
     1741 cagccaattt ttaaaacaat aaataaagtt attaatgtca ttgtcatggc gttgcttcca
     1801 taactgatca gaggcagaat caatcctttg gttggcaaaa cacccatgtt aactccgatg
     1861 tttactacaa cttgtgatgt tatccagatg ccaacagaaa aagcgaaata ggcttgaaac
     1921 actctattgt gtttcattgc atgtaaagaa atagaaaata ttctccaaat cattagggta
     1981 tatatgccaa acattaatac aacaccaatc aagcctaatt cttcagcaat gacagcaaaa
     2041 ataaaatctg tgtgagcttc aggtaaataa cccagttttt gcacactttc gcctaatccg
     2101 actccaaacc attcaccact gccaatagca atcaatgaat tcaccaactg ataactgtcc
     2161 ccgagtggat ctgcccaagg atcaaggaat gaagtaacac gatccaacct gtattggact
     2221 ttgtaaacca ataaccaaaa aactgaagct aaagctgcca gaagaacaaa gaagtattta
     2281 aacttaaagc cagcaacaaa aaataaagat gcaacaatag caagtaacac cacagtggct
     2341 ccgaagtcag gctgcaataa caataaaata caagctggta aaataaaaat catcagtttg
     2401 atgaggctta aaaaggtcat ttcaacttgt ttttgttgcc taaaacaata tcccgcaata
     2461 taaatgatca aaaaaaatcg agcagggtca ataacttgaa tggtaggtag aggtcctata
     2521 tcaacccatc tcgttgctcc attggcttct aaccctatac ctggaatgta gactagtgcc
     2581 aataacccaa ttgcaatgag agccgtaaga atacccagct tttcccagaa gtttgttggt
     2641 attaaaaaga ataaaaaccc gacaattaaa cccaaacatg ctgcaaaacc ttgtttaaaa
     2701 aaatagtgaa atgggtcggc aaaatcacga accccaatcg gcatagatgc cgaagtcatc
     2761 atcaccattc cacccattaa taaaagaaca actgaaacaa caaatagatt gtcaatattc
     2821 tcaaacagtg aaaatctttt tttcttacta aagggttggt tcaatgtaat gcctagacct
     2881 ctcttgattc gatcatctaa gctttaagct cgttaagcct actaatacca acataacagt
     2941 aattatccag aatctaacaa tgactttagg ttctgcccaa ccttttaatt caaaatgatg
     3001 gtgaatcggg gccatattaa acattctctt gccagttaac ttgaaagaaa caacctgaag
     3061 cattactgaa actgtctcca aaacaaaaat accgcccatt attaccaata caagctcttg
     3121 tctcactatc actgagacaa gccctaatgc ggcaccaaga gaaagtgcgc caacatcacc
     3181 cataaagact tgtgccggat aagtattaaa ccagaggaac cctaagcccg atccagcaat
     3241 tgctgcacaa aaaataaata cctcgccagc atctttaata taaggaacgc ctaaataatc
     3301 tgaaaaattt atgtttccag caacataggc aaaaatacct agagctgctg ctatcatcac
     3361 tgtgggcatt atggctagac catcaagacc atcagtcaaa ttcactgcat tgcttgagcc
     3421 tacaataacc aagatggcaa aaggtataaa aaatataccc atctggattg ttaaattatt
     3481 tgtgtaggga atcaccaatt ctgtctcaat aggggactga gtgtaatagt acaaaaccaa
     3541 tacagagatg agagcaagaa acaactgcat caatatcttt attgaagcct gcaaaccgtt
     3601 tttaatttta ttcagtttca tataatcatc taagaaacca atcaacccaa ataaattcat
     3661 taatatcaat gcattaatta cgtatctatt ggtcagatct ccccaaataa ctgtgctaaa
     3721 agtaattgag aagagtatta atactccccc cattgttgga gttccctgtt taacagcatg
     3781 tgattttggt ccatctgctc taatggactc accaatttca ttgaatgata attttcgaat
     3841 taatactggt ccgatcaaaa aagaaacagt tagtgcggta agagcactta atatccccct
     3901 agttgtaata taggtcagcg cattaaatgc tgataacaac tcaataagat catttgaaaa
     3961 taatagaaac actttttctc gactagttat atttttttaa ttaaaccaat ttcactttct
     4021 tttttctact tcattatctg acaattattt gattaagatt gtgtattact atccggtgct
     4081 attcccataa ttctcaatgc acccgagacg attgaagaaa aaataggtgc cgcaacttgt
     4141 ccgccaaaat acttttgacc ttgaggctca tttaccacta ctacagtaac aattcttggg
     4201 ttactaattg gagctatacc aacaaaaaat gcattatggg cattaccaga ataatcgtct
     4261 gaaaatgttt gtgctgtacc tgttttacct cccaccctat aacctttcac cttagctctg
     4321 ctcacagaat gctctacaac agcctccaac atcattaata cattagcggt agtactctca
     4381 gatagagctc gttcaccatt tggcttctcc tttacttttt caatgctaat tcgatttaca
     4441 atacccttgt tagcgattgc agaatatgcc ttagccaatt gcaaagatgt cactgaaaca
     4501 ccataaccat aggacagtgt cgcttgttta atggatctcc aattttcata acagcccagt
     4561 tctcctcttg cctctcctgg aaatccggaa gctgtgattt caccaatacc taattttgtt
     4621 aaggtgtcac aaaatatttc ttgtttcatt gctaaggaaa tttttgcagc acctgcattg
     4681 catgacctaa caagtatcat ctccaaatct aattcgcctt gacaacgatc atcaccgatc
     4741 aattttgttc caattttgaa aggcgttgta tcaacagttg atgctgatgt gtaaatacta
     4801 ttttccagag cagttgcaac agtaagagtt ttaaatgtag agccaggctc taactgatcg
     4861 gtaaccgatc tattcttgaa aaatttgggt tttctgagac tgcggatatt tggatctgct
     4921 gaaggttggt tgaccattgc cagcacttca cctgtcaata catcaagcac aactgcagaa
     4981 ccggccttgg ctctgaactc tgtaatacct gcctgtaatg ctcgatgagc aagatactgg
     5041 attctaagat ctatacttga atagaaatct tcaccatcaa ctgaattctt tatcaattga
     5101 atatcagaaa taagagtgcc aaacctgtcc ttcagtacca attttgaacc attttcacca
     5161 acaagaaaat cctgaagcaa taattccatg ccttcttgac cacgatcatc aatattagta
     5221 aacccaatca aatgaccagt gacttctcct gctggaaaat agcgtttgta ttccttttct
     5281 aaccaaagac ctttaatgtc tcccagtttt ttgatttcct tgctttgatt ctgactgata
     5341 tgacgcttta aatacataaa gctttttttt gatttttttt ctatcttttt caaaatctcc
     5401 ttagagtctg atgacaagat tcttgataat agaaatagtt tttctttatt ttttagatat
     5461 tcaagagggt tgacgaccac actatcaaca ggagtgctta ctgccaatgg tgtgcctctt
     5521 cgatcaaaga tcgatcctct attggagact attttctctt ctttgatatg tctgttgtca
     5581 gccttttgat tgtaaaattc cctttcaagg tactggagtc gaaatgaact aacaatgagt
     5641 agacaacctg cacttatgat taatgcaaag ataataaaag aacgtttcct aaaaggatta
     5701 ttgtggggtt tcttttcttt agttttggtt ttcatttacc tcaataatat ttattacatc
     5761 agcatcaagg gttttcatac ttgccttaga tctggcaata ttctctaaac gtagtccaga
     5821 agatgcttca gatgattgta tctggagttt cttccattca acttggatgg catctctttc
     5881 tttaatcaag ttctgataat cagaaaataa ttctcttttc tgatgctcca aataaactat
     5941 cccaacacta gaaatacaaa ctaaagtgaa taatacaaag gccctgatgt accatttgtt
     6001 gtcaacattc atctaattct ttccacaact ctcagaatgg cgctcctaga acgaggattg
     6061 aattgaattt cctcattaga agattttata cgttttgtaa taatttttaa tttaggcaga
     6121 tcttgttctg ttaaattggg caattttaaa agatttggat cgactcttga ttgattacga
     6181 aaaaatcgtt ttacgattct atcctctaac gaatggaaac taatgatgca taatcttcca
     6241 ccaattttta atccatgaag actaaaatct agtagttgtt tgagttgatc aagctcttga
     6301 ttaatgtaaa ttctaattgc ttggaaagtc tttgtagcaa gatgctttct actttttatt
     6361 ttttctctct gttttgcttc catgacaatc tctgataatt gcttggttgt tgttatctga
     6421 ttatctattc tgtaattaat tatggccttt gcaatttttc ttgaatagtt ttcttctcca
     6481 aatctccaaa gtacttgaga tatttcattt tcactggctg cattaatcca aatctgagta
     6541 ttagttccct cgctttgatc cattctcata tccaatggtc cttctttttg aaaactaaag
     6601 cctcgttcag cattatccaa ctgaaaagaa gaaactccta aatcggctag tatcccactc
     6661 cattgaaaat ctaaatcatg aagccctaat gtttgtctta aatctccaaa tttccctttg
     6721 acaacagtta ctctgtcctc attaacaaac ttattttttg taaaattaat tgcatcttga
     6781 tcctggtcca ttgcaataac tcttccttta gagttaagtt tgttcagaat tgcttctgta
     6841 tgaccaccaa agccacatgt tacatcaaga taaataccag acacatcctg aataaggtgt
     6901 tggacaactt tatcgacaag aacaggatga tgcatgttga tgttaaccta gactgattct
     6961 aattgaatca tgccacacca aaaaagatta tgcccgagta cgtcttaaaa atgagggaac
     7021 atctagaatg tcaatttcct cttcttcctc agaatacaga tcttcaaaca aatctttatt
     7081 acgttttgtg atcggcacat ctagaatgtc aattttatta tcagtaataa tctcactccg
     7141 attttcggtt ttgggatcca aaccggtagc gaccactgta actttaattg aatcctgcat
     7201 tgatgggtca agagcggttc ctacaacagt tatggcattc tccgatgcat aaccttctac
     7261 aatttttcct atttcctcaa attcgcccac cgtcagatcg gatcctcctg taatattgag
     7321 aaggattcct ctagcacctt tcaaattaat atctgcaagt agagggcttt ggatagcacg
     7381 ctctactgca tctctgcctc gatcatgacc agaagctgat cccactccca taatacctct
     7441 tcctgatgct gtcatcacgg tttctacgtc agcaaaatca agattgatga gaccaggaat
     7501 cgttatcaat tcggcaatgc cctgtacaga attcaagagc acatcattgg cagctccaaa
     7561 agcatcaatc atagtggttg ctttacccaa actttgaagt ttatcatttg gaataagaat
     7621 cagtgcatcg acacattttc ttagatcttc aatacccttt tctgcacatt ctactcttgt
     7681 aagcccttcc catgaaaaag gtgtggtaac aacagctact gttaaaattt caagctcttg
     7741 agcaatttct gcaactactg gacttgcccc tgtacctgtt ccacccccaa gaccggctgc
     7801 gataaatagc atatttgttt tctcttcacc tgtccctttt atagctgatg caattctttc
     7861 gcgatcttct tgagctgatg ctcgaccttt ttcaggtttg ctaccagatc ctaagccctt
     7921 ggtttcattt attccaattt gtaatttatt ttccactcct gtagcagcca atgcttgtgt
     7981 atctgtattt gcacacaaga aatcaactcc ttgaatacct tgtaaagcca tatgggaaac
     8041 tgcatttccg ccgccacctc ctataccaat aaccttgata ttggccttct gtttatcagt
     8101 atccattaat tgataagcaa attctggcat gattagatgt tcctattttt tcctataaag
     8161 acagtgttcc cagttcagaa ggcaaatttg ataagtctac atctttggtc gaaagccatt
     8221 tctcccttcc cttgttccaa tcttcatatt tccataattc aattttctta ccttgtccaa
     8281 ctaacatcac ttgtttcctt aatttggcaa aatttctcaa ctcttttggg attaaaacgc
     8341 gtccgttgct atccatttca agctcggcgg catgacccac catcaatctt tgtaatcttc
     8401 ttgatgtagg attgagtgta ggcagttcca ttatttgttt ttcaatttgc tcccagtatt
     8461 taaatggata gagcaataaa cagtggtctt gatcaattgt gcaaatcaat aaattactcg
     8521 ataaaagact caaactttct ttataccgaa caggtaaggt aatcctacct tttgtatcta
     8581 aacaaatttt atttaatcca cgaaacatct ttattaacaa taggattaac acatatatcc
     8641 cactttttac cactttatga cactatatgt aattactaac aattgtcaat aaaaaatagt
     8701 cagtaaacac attaaaacaa atgtatttag aggattcttc gaaaagatta gagttaaccg
     8761 ataagccggg ttctgtcttg gacaatcatt catctgggat atttgtcacc aaatacctct
     8821 agcaacctac cctggagtga ttacgggaaa tcgttactcc tctatttggt cttgctctcg
     8881 atggggttta ccatgccata aatgttgcca tctatgcggt gcgctcttac cacacctttt
     8941 cacccttact tcaaattgaa gcggtatatt ttctgctgca ctttccatag gcttacgcct
     9001 tccaggtgtt acctggcatc gttcccgcta gagcccggac tttcctccta aatcaaagat
     9061 ttaagcgatt gtctagttaa ctccaagtgt gattattcat tacgatgaat caaaatacaa
     9121 gtttagtatg gcttgatctc tagggctctg tgataaatat cattgcgact atatccagtg
     9181 atgattctac aaattttcac tgcctttttg atggacattt ctgataataa ttccattaga
     9241 attttatcca cttggtcaaa atcgatcgac cctttctgag gctttacacc atcaatgatt
     9301 aatacaaact cacccttgat attcgttgga ttgtcgctaa taaatgcatc tatctctgca
     9361 agagtattgc caataaaatg ttcgtgaagt ttggttattt ccttgcaaac aacccctttc
     9421 cgatcttcac caaaaatctg gctcatagtt gttatggttg cctgcaaacg atgtaccgat
     9481 tcataaaaaa tcatggtttc ttggcgcaga gccagctgtt tcaattcttt tgatttttct
     9541 gattcggctt ttggaagaaa accatgaaaa taaaatctat tggtcgccat gccagaagcc
     9601 gttagagcag ctattgcagc acaacatcct gggattggaa tgacatcaaa agaactctct
     9661 ctggtttttt taacgagcct aaaaccagga tcacttatga gtggtgtccc cgcatcggca
     9721 accaatgcta ctgttttccc atcggataac tctgttagga ttctttcagc aacttttttt
     9781 tcattatgct cattgagcaa gtggatcttt tttttaatac ttaaatgact taataacttc
     9841 ctggtgactc tagtatcttc ggcaaaaact agatcggcaa ttttcagcgt ttcaattgct
     9901 ctaagagtga tatcttgaag attgcctatt ggcgtggaaa ctaaatacag accactcatt
     9961 aaacacttaa gagtaactaa tcggtcctca tctctgcctc atcctcagtt aagctgtttt
    10021 taaatccagg ccgagaaaat actagctcca tatatttgtt catagcctca ttataattag
    10081 acggatcaat cccaaataaa tcaagtcgcc acaatactgg tgctataaca caatcaacga
    10141 cggtaaaatc atcgcttaga aaaaacctct ggaggttaaa aagatctgaa gaagatgcca
    10201 aaatagattt taattttttt ctttgagcat tttcagcttt agttccatgg gcaagatcaa
    10261 gttcaattgc aggttcaaca atatcctttt ctaaggtttt aagcgctaat cttaatttag
    10321 ccctgtctac aggactgaca ggcattaaag gaggatgagg atacctttca tcaagatact
    10381 caattatcac accagattca taaactacca attccctatc cactaaagta ggtagactgg
    10441 cgtagggatt taaagtcatt agatcttctg aaatagcatt accttcatta tgattaattt
    10501 cagttgaaat tcctttttcg gaaaggacaa aacgaacctg atgcgaacga atgcatgtat
    10561 tacctgaata caattccatc atcgaacgtc tgtttgaaac gcttgtcata cctaagtctc
    10621 ctatttattt ctaagggaat aagttcaata atatatatcg tttagtgcac ttctttccag
    10681 atatctttgt agagtaagta agaaaaaata aggaaaatta ataaaaataa gataacccat
    10741 acaccaatat tttttctttt caattgaatt ggctctgcta cataatcaag aaaattgaca
    10801 atatccaaag cgaattgatc aaactcttca tggctcaact gctctttaac taaccacaag
    10861 atattaggca tactcgttcc aggaagcacc tgattatccg ttccagtagg tgaatattta
    10921 tctatagtaa atcctcttag gaaattataa acatagtcag cactcctact tctaatgatc
    10981 aatgagagat ctggaggaga tttaccaaac catcggtcac catctgatgg caacatactg
    11041 gttgttatag gatcctgggt tgctgcttgt gtaaacatca aattctctat caattgattc
    11101 tcttcgatat ctaaatcttc agccatctga ttgtatctca tgtatttaag tgagtggcaa
    11161 cctgcacagt ggttaaaaaa gtttctagct ccccgttgca gggatgcagt atcagacaca
    11221 tcattatttg caatttgaac attggttgtt gatacatcat cagaagctac gatcaattca
    11281 gattgaataa acaatacata caaacatgta actattgttg tcttagcaat attcatttct
    11341 tgtaaatcac tctattggga acaggcttag ttttttcgaa cctagtataa aaaggcatta
    11401 gcaagaaaaa tgcaaaataa ataattgtaa aaagtctagc taaaattaca tagataccgg
    11461 tagatggctt catgcctaga taccctaaaa ctaggaaact aatggcgaat aaatacaatc
    11521 ctactttgta ttgccaacct ctgtaacgaa tggatttaac tttacattta tctaaccatg
    11581 gcaggagtac gaaacataaa actgctgcaa ccaatattat tgcccccatt aatttatttg
    11641 gaacagccct caaaattgca taatagggag tgtagtacca cgcagggaca atatgttctg
    11701 gggtctgaag gggattagca ggctcaaaat tatccacttc taaaaacaat cctcccatct
    11761 caggtgcaaa aaatattgct gcacaataaa aaataagaaa tattgtaatt cccactaaat
    11821 ccttactcgt atgataggga tgaaaagcta ctccgtcgac gggtttacct tcctcatcct
    11881 tataatcttt gatttcaata ccatccggat tattagatcc gacgtgatgc aatgccaaga
    11941 tatgagcaac taccaaagcg atgagcgcaa taggtattgc aattacatgg aaagcaaaga
    12001 acctattcag agtaatatcg gaaatataat aatctccccg aatccattct gccaaagttt
    12061 ctccaatgac tggaatagcg gtaaacaaat ttacgatgac ccctgcggcc caataagaca
    12121 tatttcccca aggaagtaga tagccaaaaa atgcttctgc catgagacaa aaataaatca
    12181 acataccaat gatccaaatg agttctcttg gttttttata cgaaccataa agcatagctc
    12241 taaacatatg caaataaaca acaatgaaaa aaaatgaaga gcctgttgag tgcatatatc
    12301 gaattaacca tccccattcg acggatcgca ttatatattc gacggagtca aaagcctctt
    12361 gaacggaagg cttgtaactc atagtcaaaa acagtcccgt aaataattgg atcactaaaa
    12421 caactgttga taaaacacca aatatgtacc aaatattaaa attctttgat gcatagtatt
    12481 cagttgcatg atctctgatc atcgaagaca agggaaatct ttcatcaatc caagtcaaca
    12541 gagtattggt aaaaatactt tggtcttttt tctgattatc agccattcac aatctcctca
    12601 gattctccaa tgatgacggt attttcatct tgatatcgat gtggcggaac ccttaaatta
    12661 gccggagccg gaaaacctga aaatactcta ccggatagat caaattttga cccatggcaa
    12721 ggacaataga agccgccttc ccaatcagat cccatgtcaa ccgatggtaa gattaatttt
    12781 tctattggag cacagcctaa atgtgtacaa actccttcca caataaaaaa ttctggcttt
    12841 aacgatctaa atttattttg ggcaaaattt ggttgtaccg acatttctga ttcaggatcc
    12901 gttaagttta gatttttctt ttgtaacgtt ttaagcatcc cttcagaacg acgtaagacc
    12961 catatcggct gacccctcca gataactttg aggatcgaac ctgcttctag tttgctgaca
    13021 tcaattttaa tcgcagcacc aagagctttg gctttcttat ttggttgaaa agaggatata
    13081 aagggtattg ttgcgaacaa aatgcccact acactggtca cgctggttgc tattgacaaa
    13141 aattttcgac gattagaatt ggtcttgatg tccttatctt cttgatccaa tattgtctcc
    13201 gagtcaagtg ccactaaatt gagggcgatt atacaccaaa taaataaaat ttaagcttct
    13261 attctcgtga tttttgcacc cactctagtc aatttattct caatgtgttc atagccacga
    13321 tcaacatggt agattctatc aataacagtt tcaccttctg caactatccc tgctaaaact
    13381 aaagcagcag aggctcttaa atctgaagcc attacaggtg ctggcagcaa tttttctacc
    13441 cctgttatat atgctttatt tctgcttaag cttatatcag cacccattct ttgtaactct
    13501 aaaacatgca taaatctatt ctcaaagatt gtctccaaaa ctgtggagct cccagaagcg
    13561 actgtattca aaaccattaa ctgcgcttgc atatcagttg gtaaaccagg atatggttct
    13621 gtcacaaaat caatgggctt gattttttct tgatacatgc tgacctcaat atcattgtta
    13681 ttaacaaaaa ttttggcacc agtttgcttc aataaattta tcactgacat taaatgttgg
    13741 ggttgcgttt ctctaagaat aagatttcct tttgtgagga tggctgcaac taagtaggtg
    13801 cctgtttcta ctctgtcggg tatattctca taaacagcac tatggagttt ttcaactcct
    13861 tcaatttcta ttttttttgt acctagtcct tttattttag ctcctgtttt cttaagaaac
    13921 attcctaagc taataacttc aggttccagc gctgcattaa ctagtatcgt ttttccttca
    13981 gccaaaactc ctgccatcat aaggttagct gttccagtaa cagagacagg attaaattca
    14041 aaatgtaccc ctttaagttt ttttgctttg gcaaatatgt accctccatc taatttaatt
    14101 tcagctccca ttgctttcaa accatcgaga tgtaaattaa ctggcctagc tcctatggcg
    14161 catccacctg gtagagaaac acgagcttct ccatatttag caagcaaagg ccccaacaca
    14221 aggattgatg cacgcatcgt tttaacaagg ttatatggtg ctaaataatt attaatattt
    14281 gatgcatcaa gttctaattt attgctatct attaaattta tagaaacacc catccctgcc
    14341 aacaatctaa tcattgttga aacgtcgcgt agatgaggaa ttttgtctaa tacgattctt
    14401 tcatcaaaca tgatagaagc agctattagt ggtaaagctg cattttttga cccagaaatg
    14461 ttaatttctc cggaaagggt ctgtccccct tgaattttga atttatccaa ttatccctag
    14521 cttctcctat aaggagttat tatgaatttt ccgctggtgt taatgcttta atagttaatg
    14581 catgtatttc tgaattcatc ctctttccca aacattcata aaccatttga tgtctctcaa
    14641 tgagagattt ctcattaaaa gcatccgaaa taacgatcgc atcaaagtga acattatcgt
    14701 tactacttac taaagcaacc gagtcttcga tagagttttc aattagaatc ttaatatcat
    14761 ctgaattcat tttatttcct taatgttatt ttacgataaa ctatttataa gtgaaaaaac
    14821 ctgaggtaat tttggttgat taattgacta gttttcttag ttaggaaata gtatttgaaa
    14881 ttaatcaaat atattacaga aaataaaagg acaaaattga taaaaaaaca agaaaaattt
    14941 aattttatag caactgggaa aaaagtatta gccatagaat cagatgccat caaagttcta
    15001 aaagatcgtt tggatagtag tttcgctgct gcgtgtgaag catgttttga atgtactgga
    15061 aaaattatcg tcatgggtat aggtaaatct ggacacatag cagataaatt agccgcaacg
    15121 cttgcaagca ccggaacccc ttcattcttt gttcatcctg gtgaagcggg ccatggagat
    15181 ctgggaatga tttcaaataa tgacattgtt attgcattgt caaattcagg agaaacagaa
    15241 gaaataattg acctgctccc agccctaaga agcatggaaa tcaaaattat tgctttgaca
    15301 ggtaacaaga attctaagat tgcaaattac tctaatattc atatagatgt aagggttgaa
    15361 gaagaagctt gcccattgaa tttagctccc acagcaagta caacagcagc attagcaatg
    15421 ggagatgcga ttgctgtagc actactggaa aaaagaggat tctctattga agattttgca
    15481 aaatcccatc ctggtggctc aatcggaaaa agaatactgc tcctggttga agatataatg
    15541 cacaccaatg atgaaattcc aattgtcttc gagaatgaaa cattagcaca aggcttaatt
    15601 gaaatgtcac aaaaagcact gggaatgaca actgttgtta ataaagaagg agaccttgtt
    15661 ggtattttca cagacggtga ccttagaaga aaacttgaag aaaatgttga catacaaaaa
    15721 accctcatgc atgaagttat gacaaaagat tgtttgactg ctgcacctaa tacacttgct
    15781 gtgaaagcta ttgaaatgat tcaggcaaat aaaattacct cactggctgt gactaaaggc
    15841 acaaaagtga taggcgctct aaatatacac gatcttttta gggcaggagt gatgtaaatg
    15901 gccccattac aatctttctc aaaagaattg gtctccattg caaaagagat aaagttgatc
    15961 gccttggatg tagatggagt tctaactgat ggatctcttt attttgatga tgacggtaac
    16021 gtagaaattt cattcaatgt aaaagatggt tatggaatag tttcacttag gaagaaatat
    16081 ggaattgaaa cgattatcat ttctggaaga tcatctaatg ctttgttgca caggatagca
    16141 gaacttaaca tctccagatt ttgggtcaat caaccaaaca aactagaagc aatcaacatc
    16201 attattcaag aaggagttat aaagccaaat caaatcctat atgtgggtga cgatatacct
    16261 gatctggagg taatcgaaaa tgtcgggctt tttgtagcag ttcaggatgc tcatgagatg
    16321 gtaaaagaaa aagcacactg gataacaaat gcttctggtg gacagggagc tgttagagaa
    16381 gtttgtgatt tagttcacta ttctattgaa aataatgatt atgactaatt atttgactaa
    16441 gcttgtcatc ttggctctac tagcgattgg aagtagtttt atttattttt ttaactttta
    16501 ttccgatgaa gatcttgaaa cacaagacat aatctcaaca gattatacga tcaagaatgc
    16561 aaaattattt ggaaccaata cagaaggtaa atttctctat aagatcattg caaaagaagc
    16621 caaagttaat cgtactaatc aacaaatata cttaaccgga gtgaatataa aatatgatac
    16681 tcaccaaaat atcgattggg taattactgc tgacaaagga caagttttat cgagctcaaa
    16741 tgtcttggtg ctgaacggaa acgtcatcct tgaaaacttg atcaaaaaga gcaatgaagc
    16801 catgccttat ttgttactac cagaactgaa tgttgctttt actaatgcta tcctcgaaaa
    16861 aactaagctc acaaccgaat accttgaagt taatccaagc acctacacca taacaaccaa
    16921 tagaaatgtt ctcattaaaa aagatgacat tacaattcaa gcacaaggtc tcacagcgca
    16981 tataaaagaa aatgaactca agtgggaaag actttgagta tattagtagt agaagattta
    17041 gaaaaaaatt acaacactaa aaaagttgtc aaaaatatta gtgttaatgt aaagagtggt
    17101 gaagttgttg gattactagg acctaatggc gctggtaaaa caactgcctt ttacatgatt
    17161 gtaggactca tctcttgtag aagaggtcga gtttcaatcg atgaatataa tttaaccaac
    17221 cttccaatgc atcatagagc aaaaataggt ctaagttact tgcctcagga agcatctatt
    17281 tttagaaaac ttacagtcga acaaaatatc gtcgctgttc tccaagcaca gcctggtttg
    17341 aaaactattg atattgagga aaagcttgaa gatcttctta cccaaatgaa tatcaatgat
    17401 ctcagaacct cattagggat tagcctttct ggaggggaga gaagaagagt agaaatcgcc
    17461 agagcattag caacaacacc aaaatttatt ttattggatg agccttttgc aggtgttgat
    17521 ccaatttctg ttatagacat ccaaagaact ataagagatt tagctcaaca agacattggg
    17581 atcttaatta ctgatcataa tgtaagagaa acacttggaa tatgtacaag agcctacatt
    17641 atgaatcaag gatccgtcat tacttctgga acgcctcagc aaatcttaga aaacaaagat
    17701 gttaaaaaat tctatttagg ggataatttt agaatgtaaa tcatattttt ttacatacaa
    17761 ttactctcag ttcttcatac aaaaaacatt cttagaaaca ggttaaacaa tgcagcagcc
    17821 agataataat ttaggtcaat tgatacaaga tcttgaaagt gatcaggtcg caggcattag
    17881 aaaaactcta acctctctta atcctccaga aattgcaaga ctgctagagt ccttaacagt
    17941 tggtaaaaga aaaatcatct ggcaactggt agatcaggaa gatgaaggtg aaatattgaa
    18001 ggaattagtg gatgacgtta gacaaaatct aattgaagag atggatgcct cagaactcat
    18061 cgcagtaacc caggatatgg aattggatga tttagctgat ctcttaatag atataccaga
    18121 agcaattaca gaacaaatca ttacagcctt ggatcaacaa gatcaagtca gacttg
//
LOCUS       DASCTH010000201         3870 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000320577, whole genome shotgun sequence.
ACCESSION   DASCTH010000201 DASCTH010000000
VERSION     DASCTH010000201.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000320577"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="QGH35_04315"
     CDS             complement(<1..248)
                     /locus_tag="QGH35_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HJL81792.1"
                     /translation="MKSVKKIYNSGDDDLFYIDYIPPMKSKQKLFEPGAIIRRMGEPV
                     DMIYYLESGFLTLSKTSNSGKRQVINFIFPGEYCGITN"
     gene            complement(543..1043)
                     /locus_tag="QGH35_04320"
     CDS             complement(543..1043)
                     /locus_tag="QGH35_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HJL81793.1"
                     /translation="MIRRFAESSQILSKILEKDTRFLDKGTYLLEIISDLEQPAFKIG
                     SLGCMDLNPGTYYYIGSAFGPGNLKARLERHLASNKNKHWHIDYLLDHLVVETIWCCC
                     GDRENEHIWAQSFASKDVMEIPLRGFGSSDCSCETHLFFTQKKLSGIRIKRLLSGTIS
                     GEVFSS"
     gene            complement(1003..2145)
                     /locus_tag="QGH35_04325"
     CDS             complement(1003..2145)
                     /locus_tag="QGH35_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008490267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL81794.1"
                     /translation="MNLNYSDEQKMFRDQVQKFCESDYSMDKRGEIIKSQDGFDKDFW
                     KLFSELGWLSMPFKEDAGGFGYGPIELSILFEEFGKVLVIEPYLSSVVLSGMILDNSN
                     FKNRIELIESITSGTQQLSVAYIESNQNFDHSLPATTAVSDGENLIICGTKSLVLNGG
                     NADQIIASVMLDGEFALVLIDKENTGYACQAYPTVDGQTCADITFNSVSISNKSIIAR
                     GEEAVSLLENAIHLATLCICAESIGCMTACYLKTVEYTKGREQFGQPISNFQVLQHRM
                     VDMFIHTELCKSLLFKAMLEFDSNADTKNQSVSALKFQIGISGKFVSQQAIQLHGGMG
                     VSNEMLIGHYLKKIVAIDALFGNGNHHLNNYSSHDQKIRRVIPDPV"
     gene            complement(2148..3317)
                     /locus_tag="QGH35_04330"
     CDS             complement(2148..3317)
                     /locus_tag="QGH35_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL81795.1"
                     /translation="MEINFSAQDHQFREEVRTWIKDDYPAHVKRKQDNGELLTKEDVI
                     DFHKAVSAKGWMGFNWPVEYGGTGWTSTQIYIFQNEFGLAGCPPLLAFGVNMVGPVIY
                     TFGSEDQKKRFLPDILNFNTWWCQGYSEPGSGSDLASLKTKAVKAGDHYIVNGSKTWT
                     TLAQSANWIFCLVRTETTQKKQEGISFILIDMNTPGVEVEPIITIDGAHEVNAVFFTD
                     VKVPVENLVGEEGKGWTYAKFLLAHERFGIAAVGASKRQLNRLKEVVAVLDNPDLDRK
                     VTELEINLTALEMTELRLLSALESGAHPGAESSILKICGTEIQQRLTELFLEASGEYA
                     LLYPGPHGYDDQAKPVGPDYAANSVFRFLNYRKTSIYGGSNEIQKNIIAKFILGL"
     gene            complement(3422..>3870)
                     /locus_tag="QGH35_04335"
     CDS             complement(3422..>3870)
                     /locus_tag="QGH35_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006365866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HJL81796.1"
                     /translation="SGIVFLLIMRTLFAIFWLAAGVNKINSGWVTSDILKEVFLQRLT
                     ELPPDSFAVLYLSSFAIPLHTLIAWIITLGELYSAIGLLIGLTTRWSAGVSLFILINF
                     AIGGYYDASLIPFFILVALFIYYPSGRWLGFDKLLHEKYPSSIWFR"
BASE COUNT         1227 a          781 c          649 g         1213 t
ORIGIN      
        1 tgattggtta ttccacaata ttcgcctgga aaaataaagt tgatgacctg tcttttccca
       61 ctattggaag tttttgagag cgtgagaaat ccagactcga gataatagat catatcgacc
      121 ggctctccca ttctccggat gatagctcct ggctcaaata atttctgttt ggatttcata
      181 ggcgggatgt aatcgatata gaatagatca tcatccccac tattataaat cttttttacg
      241 cttttcataa aggctacctg ctacctcaat aattcaaaag actatttatt ttactatagg
      301 atgcgaagat tttcagagca ttgatctgaa tcaaatggtc tctgacataa atcaatgctt
      361 ctatgagcta aagattagat gatgtctatg gatcttagat aatgagtgaa ttataaaatg
      421 aaaatgaaaa attcagaaca tcatggtgac atggatgatc tagttagctt ggttaccatt
      481 tctttaacag taatcgtagc attggtaatc ggcattattt gctttactta atcaacaact
      541 aattaagaag aaaaaacttc gccactgatc gtacctgaca acaagcgttt tatgcgaata
      601 ccagatagtt ttttttgagt aaagaacaag tgtgtttcac aactgcaatc cgatgagcca
      661 aatcccctta aaggaatctc catgacatcc ttggaagcaa aactttgagc ccatatgtgt
      721 tcattctctc tgtctccgca acagcaccag atagtttcaa caacaagatg atccaataag
      781 tagtcaatat gccagtgttt gtttttatta gaagcaagat gtcgttctaa tcttgctttt
      841 agattgccgg gaccaaaagc actaccaatg tagtaataag tacctggatt gagatccatg
      901 catcctaaag aacctatctt gaatgcaggt tgttctagat cggatattat ttctagaaga
      961 taagtgcctt tatcaaggaa tctagtatct ttctcgagga ttttagacag gatctgggat
     1021 gactctgcga atcttctgat catgacttga ataattgttg agatgatgat ttccattacc
     1081 aaataaagcg tctatggcaa ctattttttt aaggtaatgg ccaattaaca tttcattgct
     1141 cacacccatc ccaccatgaa gctgaatagc ttgttgggat acaaatttac ccgagatccc
     1201 tatctgaaat ttaagtgcag aaactgattg attttttgtg tctgcatttg aatcaaactc
     1261 aagcattgct ttgaataata aggatttaca aagttcagta tgaatgaaca tatctaccat
     1321 tctatgttga agcacctgga agttgcttat tggttgaccg aattgttctc tacctttggt
     1381 atattcaaca gtctttaaat aacaagccgt catacaccca atactttcag cacaaatgca
     1441 tagggtagct aagtggatcg cattttccag aaggcttaca gcctcttcac ccctggcaat
     1501 gatggatttg ttactgatac taacactatt aaatgtaata tcagcacaag tctgaccatc
     1561 aacagtagga tatgcttggc aagcatagcc tgtattttct ttatcaatca acaccagcgc
     1621 aaattctcca tccaacatta ctgaggcaat aatttgatcg gcattacctc cgtttaaaac
     1681 tagggattta gttccacaaa ttataagatt ttcaccatca gaaacagcgg tagtagcagg
     1741 tagggaatga tcaaaattct gatttgattc tatgtaggca actgatagct gttgagtgcc
     1801 gcttgtgatg gattcgataa gctcaattcg attcttaaaa ttggagttat caagaatcat
     1861 ccctgataaa acaacactac ttaaataagg ttcaatcaca agaaccttac caaattcttc
     1921 aaacagaatg gaaagctcta ttggtccata cccgaatcca cctgcatcct ctttgaaggg
     1981 cattgatagc catccgagtt ctgaaaaaag tttccaaaaa tctttatcaa aaccatcttg
     2041 ggatttaata atttcccctc ttttatccat cgaataatct gattcacaga atttttgaac
     2101 ctgatctcga aacatttttt gttcgtctga ataattcaaa ttcatggtta taaccctaaa
     2161 ataaattttg caataatatt tttttgaatc tcattactgc caccataaat ggatgttttt
     2221 ctatagttta agaacctgaa tactgaattg gcagcataat caggtccaac aggctttgct
     2281 tgatcgtcat aaccatgtgg cccaggataa agaagagcat attcacctga agcttccaga
     2341 aacaattccg taagtctttg ttgaatttcg gtaccacata tttttaagat ggaagactcg
     2401 gctccgggat gagctccgct ctcaagagca gatagtaatc tcaattctgt catttccaaa
     2461 gcagtcaaat taatctcaag ttcagtgact ttcctatcta gatcagggtt atccagtact
     2521 gctacaacct cttttagtct atttaattgg cgcttagagg caccgacagc agcaatacca
     2581 aatctctcat gtgctaataa aaatttagca taggtccaac ctttaccttc ttcaccaacc
     2641 aggttttcta caggaacttt tacatctgta aagaaaacag catttacttc atgtgctcca
     2701 tcaatggtaa tgataggttc cacttcaacg ccaggagtgt tcatatctat gagaatgaat
     2761 gaaattcctt cttgtttttt ttgtgtggtt tccgttctaa ctaggcaaaa aatccaatta
     2821 gcgctttggg ctagagtggt ccatgttttt gagccattga caatgtaatg atcgcctgct
     2881 ttaactgctt tagtcttcag tgatgctaaa tctgaaccag atccaggttc tgaatatccc
     2941 tgacaccacc aagtattaaa gttcaatata tctgggagaa accttttctt ttggtcttcg
     3001 ctaccaaatg tataaataac cggtccaacc atattcacgc caaaagccaa tagaggcgga
     3061 caaccggcaa gaccaaattc attttggaat atgtaaattt gagtcgatgt ccatccggtt
     3121 cctccatatt caacgggcca gttaaaaccc atccaacctt tagcggacac tgccttgtga
     3181 aaatcaatta catcctcttt agttaacagt tctccattgt cttgtttacg tttaacgtga
     3241 gctggataat cgtctttgat ccaagttctc acttcttctc taaattgatg atcttgtgct
     3301 gagaaattaa tttccatttt ttgcctttat gattacttct acctattgtt cagcaataat
     3361 ttatatagaa cccgtgattt aaccaaaatt tattccggtt gaacactttt tttacagtaa
     3421 ttcacctaaa ccaaatactt gatgggtatt tttcatgtaa taatttatca aagccaagcc
     3481 atcgacctga agggtagtaa ataaataaag cgactagtat aaaaaatggg ataagggaag
     3541 catcgtaata gcctccgata gcaaaattta tcaatataaa taaactgacc ccagcagacc
     3601 atcgagtggt taatccaatt aataacccta tagcactata caattcaccc aaagtaataa
     3661 tccatgctat taacgtatga agaggtatgg caaaactgct taaatacaat accgcaaaac
     3721 tatcaggcgg aagttcagtc aaacgctgga gaaaaacctc ttttaaaata tcgcttgtca
     3781 cccagccact gtttatttta ttgactcctg ctgctaacca aaaaatagca aataacgtcc
     3841 tcatgataag aagaaaaaca ataccgctca
//
LOCUS       DASCTH010000202         1979 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000320616, whole genome shotgun sequence.
ACCESSION   DASCTH010000202 DASCTH010000000
VERSION     DASCTH010000202.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000320616"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="QGH35_04340"
     CDS             <1..936
                     /locus_tag="QGH35_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013799.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HJL81797.1"
                     /translation="ATGVYPNRHGILHNSFIDAQKGFFNYDADANWIETPPIWVLAEQ
                     QGITTAVFFWVGSETDWHGIGAFYRKAPFDPNITEEEKIDQILQWLDMDDDQRPRLIM
                     SYWDGTDDLGHIDGPESPRVSQQLLRQNNLLQNLITAIDVREAWDYVTLIVVSDHGMT
                     EVTNYIDLNNLLRISGIEGNITSGPAVAHIFLAADDIERARKFFSKQEHLKVYTKRYL
                     PDTFHLYHPTRTGDLVITTEAPNMFSSFGRPKGMHGYKPETSDMWGIFYALGAAVVPK
                     EIGSIHQVDVAPTIAEILGIEMPQETQGQSIDLGN"
     gene            complement(943..1602)
                     /locus_tag="QGH35_04345"
     CDS             complement(943..1602)
                     /locus_tag="QGH35_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012264.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HJL81798.1"
                     /translation="MLILFTPRAFSLTADPEAGKDKATTCSMCHGEDGNSIEPEWPNL
                     ASQNKQYIINSIKAFQSGARGNCYSCHGQDDNDVELGWSSTTGQHIVAVMKTQLMTLT
                     NQDIENIAAYYNNQSTTALETDSTLANQGESLYTTGNKLTGVSACIGCHGPTGRANGD
                     LGYPSLAGQHALYTENTLRNYASGERQTEANNLMQILSASLSETEIRAVSAYIQALGS
                     Q"
     gene            complement(1728..>1979)
                     /locus_tag="QGH35_04350"
     CDS             complement(1728..>1979)
                     /locus_tag="QGH35_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HJL81799.1"
                     /translation="PQWQIEALRRFQIPLEMQEKFGYAAITEDDKKKILGLNAAKLYQ
                     IDVSETRKQIATDKMTHLKEIYLEEGGRPSNNQYGWVVT"
BASE COUNT          543 a          415 c          415 g          606 t
ORIGIN      
        1 gcaactgggg tttatccaaa tcgccatgga atcctgcata attcttttat tgatgcccaa
       61 aaaggatttt tcaactacga tgccgatgcc aactggattg aaactccacc catttgggtt
      121 ctggcagaac aacaaggcat cactacggct gtttttttct gggttggatc cgaaactgat
      181 tggcacggta taggtgcttt ttatcgaaaa gcaccttttg atcccaatat cacagaggaa
      241 gaaaaaattg atcaaattct acaatggctt gacatggatg atgaccagcg acccaggtta
      301 attatgagtt attgggatgg gaccgatgac ctaggacaca ttgatggtcc agaaagccca
      361 agagtttctc aacagctgct gcgacagaat aatcttttgc agaatttaat cactgccatt
      421 gatgtaagag aagcttggga ttatgtgaca cttatcgtcg tcagtgatca tgggatgacc
      481 gaagtgacca actacattga cttaaataat ttgttaagaa tttccggaat tgaaggtaac
      541 atcacgtcag gtccggctgt agcacacatt tttttagcag ctgacgacat tgagcgagcc
      601 agaaaatttt ttagcaaaca agaacacctt aaggtgtata ccaaaaggta tcttccggat
      661 accttccacc tctatcatcc gaccagaact ggcgatttag tgattactac cgaggcaccc
      721 aatatgttct ctagtttcgg tcgacctaag gggatgcatg gctacaaacc agaaacgagc
      781 gatatgtggg gtatttttta tgctctagga gctgcggttg tacccaagga gataggatcc
      841 attcatcagg tcgatgttgc ccccactatt gcagaaattc taggtattga gatgcctcaa
      901 gagacccaag gtcaaagcat agatttgggc aactagatta tcttattgcg atcccaaagc
      961 ttgaatgtat gcagatactg ctcttatctc tgtttcggat agactggcag ataggatttg
     1021 cataaggttg ttagcctccg tctgtctctc tcctgaggca tagtttcgta gtgtattttc
     1081 agtatacaat gcgtgttgcc cagcaagaga aggataacct aaatcgccat tagctctgcc
     1141 agttggtcca tggcaaccaa tacacgcact aacaccggtt aacttgttgc cagtagtata
     1201 aagggactca ccctggtttg ctaaagtgct atctgtctcc agtgctgtag tgctttgatt
     1261 gttgtagtag gcggctatat tttcaatatc ttgattggtt aatgtcatca gttgtgtctt
     1321 catcaccgcc acaatgtgtt gtccagttgt actagaccac ccaagctcga catcattatc
     1381 atcctggcca tggcacgaat aacaatttcc tcgagcccca ctttgaaacg ctttaattga
     1441 attaataatg tactgtttat tttggctagc caaattaggc cattcaggct ctatgctatt
     1501 accatcctcg ccgtgacaca tggaacaggt agtagcttta tctttcccag cttctggatc
     1561 agctgttaag gaaaacgccc tgggtgtgaa taatataagc accaggatta caatgaaagg
     1621 aatgcatcta tttattattt tgatgagagc tatcacagtt ttttctataa tttgatctat
     1681 ttaatgtaca caagactgag acagttaaat cagctacgaa gtttgttcta agtgacaacc
     1741 catccgtact ggttattact agggcgacct ccttcttcaa ggtatatttc ttttaaatgg
     1801 gtcattttgt ctgtagcgat ctgttttcta gtttcagata catcaatctg gtacagcttt
     1861 gctgcattta aacctaggat tttcttttta tcatcctcag taatggcggc atagccaaat
     1921 ttttcttgca tctccagagg tatttgaaat cgccttagtg cttcaatttg ccattgagg
//
LOCUS       DASCTH010000203         1557 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000332099, whole genome shotgun sequence.
ACCESSION   DASCTH010000203 DASCTH010000000
VERSION     DASCTH010000203.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000332099"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="QGH35_04355"
     CDS             <1..771
                     /locus_tag="QGH35_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJL81800.1"
                     /translation="QQYNQKHNFVSIQLNLLLAEQQESSAIINELTKTLEYLEEGTVY
                     LSGLVNLDMENQINVQNFLINWLPKQRNRYVLEMLGTKRTTSIPEGHKTLEQAFPDIV
                     VIDLPPLRQHLQDVPDLLKIFMNYFVTQEGLSQRRFSLAAINMLSKYAWPGNLKQLKN
                     MVHTILIRSNKEIINIEEVEETLTLQGPSGDLLVQNDIMSMTMTEAKKEFEKAFLLRQ
                     LELVGWKVTELARRVDMERTNLYRKLSSLGIEYKNKKQ"
     gene            complement(800..>1557)
                     /gene="argS"
                     /locus_tag="QGH35_04360"
     CDS             complement(800..>1557)
                     /gene="argS"
                     /locus_tag="QGH35_04360"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HJL81801.1"
                     /translation="LREDGSSTYFASDVAYHLDKRDRGFDLLLNVLGSDHHGYIPRLK
                     AGLEAMNHPAESLEVILIQFVSLFRGNKKIQMSTRTGNFIPLMELLEEVGKDAARFFY
                     ISRSNDQHLNFDLDLAKAQNNDNPVYYIQYAHARIHTVFQELRRNDFNFDKNSGISNL
                     GLLSTNHETEIITKLGDYPNVITLSADNYSVHSLANYLLEVAQLFHSYYNAHRFIVAD
                     SEIRNARLALIAAIAIVVKDGLSLLGVSAPEKM"
BASE COUNT          545 a          270 c          285 g          457 t
ORIGIN      
        1 caacaatata atcaaaaaca taactttgta agtattcagt tgaatttatt gcttgcagaa
       61 caacaagaaa gcagtgctat catcaatgag ctgacaaaaa ccttagagta tttagaagaa
      121 ggcacagttt atctttcagg gctagttaat ttagacatgg agaaccaaat caatgtccag
      181 aattttttaa ttaactggtt gcccaaacaa agaaatagat atgttttaga gatgcttggt
      241 accaaaagaa caacatctat tccagaaggc cataaaacac tagaacaagc ctttcctgat
      301 attgtagtta tagatttgcc ccctttaaga cagcatcttc aagatgttcc tgatcttctc
      361 aaaatcttta tgaattattt tgtaacacag gaaggattgt ctcaaaggag atttagtttg
      421 gcggctataa atatgctttc taaatacgca tggccaggga acttaaaaca attaaaaaac
      481 atggttcata caattttaat cagatcaaat aaagaaatca taaacattga ggaagttgaa
      541 gaaacattga ctcttcaagg cccaagtggt gatctgctgg ttcaaaacga tattatgtct
      601 atgaccatga cagaggctaa aaaagagttt gaaaaagctt ttctcttaag acagttggag
      661 ctcgttggtt ggaaagttac tgagcttgca aggcgtgttg atatggaaag aactaatcta
      721 tacagaaaac tcagctcgct cggtatagag tataaaaata aaaagcagta atttttttaa
      781 aagatagtct ctgttctagc tacattttct ctggagcaga aacccccagc agggacaaac
      841 catctttaac aacaattgct atggccgcaa ttaacgctag acgagcattt ctaatctcac
      901 tgtcagccac tataaatcgg tgtgcgttat agtaggagtg aaacagttga gcaacttcaa
      961 gtaaataatt agccagagag tgaacggaat aattatctgc agataatgta ataacgttgg
     1021 gataatctcc aagcttggtg atgatttcag tttcatgatt tgtggataat aaacccaggt
     1081 ttgatattcc agaattttta tcaaaattaa aatcattacg gcggagctct tgaaatactg
     1141 tgtgaatacg agcatgagca tactgaatat aataaacagg gttgtcattg ttttgtgctt
     1201 ttgccaaatc aagatcaaaa tttagatgtt ggtcattaga tcgtgaaata tagaaaaacc
     1261 ttgctgcatc ctttccaact tcctctagta gctccataag tggaataaag ttaccggtgc
     1321 gagtagacat ttgaattttt ttattccctc taaacaatga aacaaactgt atcaaaataa
     1381 cctccaagct ctctgctgga tgattcattg cttcaagccc agcttttaat ctaggtatat
     1441 agccgtgatg atctgaacct aaaacattta acaacagatc aaaaccacga tctcgtttgt
     1501 ctaaatggta agcgacatca gatgcaaaat aagtggagct accatcttct ctgagaa
//
LOCUS       DASCTH010000204         2342 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000332166, whole genome shotgun sequence.
ACCESSION   DASCTH010000204 DASCTH010000000
VERSION     DASCTH010000204.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000332166"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(102..177)
                     /locus_tag="QGH35_04365"
     tRNA            complement(102..177)
                     /locus_tag="QGH35_04365"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(142..144),aa:Phe,seq:gaa)
     gene            complement(226..501)
                     /locus_tag="QGH35_04370"
     CDS             complement(226..501)
                     /locus_tag="QGH35_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HJL81802.1"
                     /translation="MSRQVFCEHFQKELEGLDRPPYPGELGEKIYQSVSKQAWNTWIE
                     HQTMLINENQLSMLDSKARKYLQEEMTKFLFGKGSEKPSEYVPPDIK"
     gene            complement(501..1607)
                     /gene="mutY"
                     /locus_tag="QGH35_04375"
     CDS             complement(501..1607)
                     /gene="mutY"
                     /locus_tag="QGH35_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HJL81803.1"
                     /translation="MAQDQYNYLATRLVNWWQQFGRKHLPWQSNPSKYKTWISEIMLQ
                     QTQVTTVEPYFARFMERFPTVTELASSSLDEVLQLWSGLGYYARARNIHRSAQFIVKQ
                     HQGELPSDYDNLLTLPGIGKSTAGAILSLSGIEPKPILDGNVKRVLSRFFGVKGWSGK
                     SKVSKELWDLSAKSLPADHFQIYTQGIMDLGAMVCLPKNPNCRICPIVSRCYAYQNDL
                     VGEIPKKKLRKKKRTESKCLLMLVFDGRSSGKDLFLEKRVGQELWGGLWSLPEVELSE
                     DPRAWCERHFTNKVLSIDPWEPIKQPLSNYILYIHPIKIRMDGRFDYRSTNYRPRAVP
                     EPTMNTGCFTLSKNLDSSFGFPVPVNRLINQLTQ"
     gene            complement(1625..2254)
                     /locus_tag="QGH35_04380"
     CDS             complement(1625..2254)
                     /locus_tag="QGH35_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81804.1"
                     /translation="MFVFWLGTDMGVMLCSKKSIDSTLSIETRFQLLEMALKIELLPR
                     VMWVMALPFGVHLSKSLGYIDPNVITVALMWVFVIAWLIINVGGAANLDKPWGQQLSK
                     LNRFITASLGVGLIIVAISSFMGFGPYEANSIALKVGLYGLVNITILGIEIAFFPLGA
                     SFERLATEGSSPELEQSISSGMGTTLMWVHITYIMIFVVAFIGVTKIAG"
BASE COUNT          704 a          502 c          426 g          710 t
ORIGIN      
        1 tacacacttt gacctcataa caagtgttta gccttctaaa ttgaatcttt tcaacaataa
       61 taaagaatca ttgaatatca ttatttaatc tttagcgggt gtggtggcca gaggtggaat
      121 tgaaccaccg acacgcggat tttcagtccg ctgctctacc aactgagcta tctggccttt
      181 gcgtcggttc attttataga ataatggaaa caaaaccata aaaaatcact ttatatcagg
      241 agggacgtat tcgcttggtt tttcagagcc tttgccaaat aaaaatttgg tcatttcctc
      301 ctgtaaatat tttctggctt ttgaatctaa cattgacagt tgattttcat tgatcagcat
      361 tgtttggtgt tcaatccaag tattccaagc ttgttttgaa actgattggt agattttctc
      421 tcctaattct cccggatatg gaggtcgatc tagaccttcc agttcctttt gaaaatgttc
      481 acaaaatact tgtctgctca ttattgagta agttggttaa tcaaccggtt gaccggaacc
      541 ggaaatccaa aactagagtc cagattcttg cttaaggtaa aacatcctgt gttcattgtg
      601 ggttcaggaa ccgctcttgg acggtagttt gtactcctat aatcaaatct cccatccatc
      661 ctaattttaa ttgggtgtat atacaaaata taattgctga gtggctgctt aataggctcc
      721 catgggtcaa ttgataagac tttattggtg aaatgccgct cacaccaagc tctgggatct
      781 tctgaaagct ctacttcagg caaggaccat aagccacccc atagctcctg cccgactctc
      841 ttttccaaga acaaatcctt tccactagat ctcccatcaa aaacgagcat aagcaagcac
      901 ttcgattctg ttcgcttctt ttttctcagc ttcttcttgg gaatttcacc aactaagtca
      961 ttctgatatg cataacacct gctcacaatt ggacaaatgc gacagtttgg attttttggc
     1021 aaacaaacca tagcccccaa atccataatt ccttgtgtgt atatttgaaa atgatcagca
     1081 ggtaaagact tggcagacaa atcccataat tctttgctga ctttactttt accagaccaa
     1141 cctttaaccc cgaagaatcg gcttaaaact cttttaacat tcccatccag tatcggtttt
     1201 ggctcaatac cactgaggga taaaattgca cccgctgtcg atttacctat accaggtaaa
     1261 gttaacaggt tgtcataatc tgagggcagt tcaccttgat gttgtttaac tatgaattga
     1321 gcagatcggt gtatgtttct cgctcgggca taataaccta gccctgacca taattgcagg
     1381 acctcgtcta atgaggatga tgcgagttct gttactgtgg gaaatcgctc cataaatcga
     1441 gcaaaataag gctcaacggt ggttacttgg gtttgttgta gcatgatttc agaaatccaa
     1501 gttttgtatt ttgatggatt gctttgccaa gggagatgtt ttcttccaaa ttgttgccac
     1561 caattgacca accgagtagc gaggtaattg tattgatctt gtgccacaga aagggcttgt
     1621 gatactagcc tgcgattttt gttacgccaa taaaggccac tacaaaaatc attatatagg
     1681 tgatgtgaac ccacattaga gtagtgccca tgcctgagct tatcgattgt tccagctctg
     1741 ggctagagcc ttctgtagca agtctttcaa aagatgcgcc taggggaaaa aatgcaattt
     1801 cgatccctaa aatcgtaata ttgaccagac cataaagacc cacttttaat gcaatagagt
     1861 tggcttcata gggtccaaaa cccataaacg aagatatagc aacaataatt aagccaacgc
     1921 ccaaagacgc agtaataaat cgattcaatt ttgataactg ttgtccccat ggcttatcca
     1981 agtttgcagc accaccgaca ttaatgatta accaggcgat aacaaaaacc cacattaaag
     2041 ccactgttat aacattggga tcaatataac cgagtgattt agataaatgg actccaaacg
     2101 gcaacgccat gacccacata acccttggta ataactctat ttttaaggcc atttctaata
     2161 attgaaaacg tgtttctata ctgagagttg aatctatgga tttcttggag caaagcatta
     2221 ctcccatatc cgtaccaagc caaaaaacaa acatacatat atgaaggaat ttccaaaact
     2281 catatgaatc cataacacaa cctcatgttg tagaattata agaattaatc tcagtttgct
     2341 tg
//
LOCUS       DASCTH010000205         2832 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000335716, whole genome shotgun sequence.
ACCESSION   DASCTH010000205 DASCTH010000000
VERSION     DASCTH010000205.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000335716"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="QGH35_04385"
     CDS             <1..966
                     /locus_tag="QGH35_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81805.1"
                     /translation="ALGKVGLATTKGRTGNDKEFIPKISVKYLLSDESMIYGLYTKGY
                     RPGGVNRSRGDPFFPNAYGPDMMENREIGYKSTFAQGRGRLNVIAYDMEWSEYQLEQV
                     DPSQVPCDSNGNAVDLGSPDADAGVKTPGVCGQPWQNLVANTGQAHITGVNLELDYRV
                     NERLSLGFNVEVKEAETDEDATGIVESGLQLPLSADGKGAIWLDYTWEASQWGSNYGF
                     ARFQYSKQGAIWNNLDGGAVADSMNPRQRVPGYAIADARIGLQGDDWEVSLFVNNLFD
                     ERAAYTYSGGQFLWGMGSSADGVDHHQLVYVNRPREVGIRFTQSW"
     gene            complement(1024..1962)
                     /locus_tag="QGH35_04390"
     CDS             complement(1024..1962)
                     /locus_tag="QGH35_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81806.1"
                     /translation="MKSRLPKLILTSVVRGSQQGESHGGIYTVDFEHQQGQQHVDWNT
                     SDIDFEGRGADRGLRGITFDGDEIYIAASDELFCYDQTFTIQNSYKNPYLKHTHEICR
                     MERRLLLTSTGFDSLLAFNLDTKEFDWGFQLRQEYGQWTGHTFDPQSNHGPRLVNDFH
                     INMVHVDDKGIFLSGLHTNGLLFLTSSNEIKTVCDLPAYAHNPQLWKDGVLFNDTASD
                     CVRYVPRQGQEQAYKIITYQEKDIQFSGIDDSNIARQGFGRGLCTIGDRFVAGGSSPS
                     TVTLYDFEATMVVGSVNLTMDIRNAIHGLEIWPYQD"
     gene            2075..>2832
                     /locus_tag="QGH35_04395"
     CDS             2075..>2832
                     /locus_tag="QGH35_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HJL81807.1"
                     /translation="MAKKLSITRRDFMNGFAMSLTAGTALSPIELLAMNQQISSSSLY
                     PPELTGLRGSHPGSFEVAHALAMNGASWSDPSDQTDHDYDVVIVGGGISGLSAAYLYR
                     QRHGGDPRILVLDNHDDFGGHAKRNEFNVDGKKLICYGGSQSIAEPGSWSVVGKKLLK
                     DVGIHTQHFYDYFDRNYYKDRKLGRGLYFSHDQYDKDHVSDNILGVEIGDNEQEIIDI
                     INEYPIAKASKVALFRLLSDKKNYLKGMGSREDR"
BASE COUNT          816 a          554 c          668 g          794 t
ORIGIN      
        1 gctttaggta aagtcggtct agctaccaca aaaggacgta cgggtaatga caaggaattc
       61 attccaaaaa tcagtgttaa atacctgttg agtgacgaaa gtatgatcta tgggttgtac
      121 accaagggtt atcgacccgg gggtgttaat cgtagtcgtg gcgacccatt ctttccaaat
      181 gcttatgggc ctgacatgat ggagaacaga gaaattggct acaagtcaac ttttgcccaa
      241 ggcagaggac gtctcaatgt cattgcctat gatatggaat ggtctgaata tcaactggaa
      301 caggttgacc catcacaagt gccttgtgat tccaacggca acgccgtaga tttaggatcc
      361 cctgatgcag atgctggtgt aaaaacaccc ggtgtttgcg gacagccatg gcaaaacttg
      421 gtggccaata caggtcaagc gcacatcaca ggcgtgaacc ttgaattgga ctaccgggtg
      481 aatgaaaggt taagccttgg tttcaacgtc gaggtcaagg aagcagagac tgatgaggat
      541 gcaactggta tcgttgaatc tggtctacaa ttaccgttat cagccgatgg caaaggtgct
      601 atctggttag attatacttg ggaagcctca caatggggat caaactatgg tttcgcaaga
      661 ttccaatatt ccaaacaagg cgctatttgg aataatctag acggtggagc cgtagccgat
      721 agcatgaatc ctagacaacg cgttcctggc tatgcaattg cagatgctag aatcggattg
      781 caaggtgatg attgggaggt ctcattgttc gtcaataacc tcttcgatga acgcgccgcc
      841 tatacttaca gtggcgggca attcttatgg ggaatgggtt cctcagcaga tggtgttgac
      901 catcatcagc tagtttatgt gaaccgccca cgtgaagttg gtattcgatt cacccaatct
      961 tggtgattgg atagcaagct caagtgataa gataaaaaga accgggcatt tgcccggttt
     1021 tttttaatcc tgatagggcc agatctctaa cccatggatt gcatttctaa tatccatcgt
     1081 caaattaact gatcctacaa ccatagtggc ttcaaaatca tagagagtca cagttgatgg
     1141 cgaagaacca ccggcaacaa atcggtctcc gattgtgcac agaccccggc caaaaccttg
     1201 gcgagcaata ttagagtcat cgataccgga aaattggatg tctttttctt gataggtaat
     1261 aatcttatag gcttgttctt gtccttgacg tggcacataa cgaacgcagt cgctagcggt
     1321 gtcattgaat agcactccat ctttccaaag ctgcgggttg tgtgcgtaag cagggagatc
     1381 gcaaacagtt ttgatttcat tgctactggt taaaaacaac aatccattgg tatgcaagcc
     1441 agagagaaaa attcccttgt catcgacatg gaccatgtta atgtgaaaat cattgactaa
     1501 tctgggccca tgattacttt gtgggtcaaa agtatgaccg gtccattgac cgtattcttg
     1561 tcgtaactga aatccccagt cgaattcttt agtatccagg ttaaaagcca gcaaactatc
     1621 aaaacctgtc gatgttagta gtaggcgccg ttccattctg catatttcat gggtgtgttt
     1681 taaataggga tttttgtaag agttctggat tgtaaaagtc tggtcatagc aaaacagttc
     1741 gtcgctggct gcaatataaa tctcatcacc atcgaacgta attcctctta agccccgatc
     1801 agcccctcta ccttcaaagt caatgtcact ggtattccaa tcgacatgct gttgaccttg
     1861 ttgatgttca aagtcaacgg tatagatgcc accatggctt tcaccttgtt ggcttcctcg
     1921 aacgacagag gtgaggatta atttaggcag tcgacttttc aatgttaaaa aatttttaaa
     1981 ggtatcaagg taaacttcaa tcagtatttg cctatactac caatagagtt ctagaatgta
     2041 atgagaattt aagtctacca agaggatgtg atttgtggct aaaaaattaa gtattactcg
     2101 gcgtgatttt atgaatggat ttgcaatgag tttgactgcc gggacagctt tatcgcctat
     2161 tgagcttttg gccatgaatc aacaaataag tagttcgtct ttgtatccac ctgaactcac
     2221 tggtttaaga ggcagtcatc caggttcatt tgaggtggct catgccctgg caatgaatgg
     2281 cgcaagttgg tcagaccctt ctgatcagac tgatcatgat tatgatgtag tcatcgttgg
     2341 tgggggtatt tcagggttgt cggcagcata cctctacagg caacgccacg ggggagatcc
     2401 aagaatattg gttttagata atcatgatga ttttggtggc catgcaaaac gcaatgaatt
     2461 taatgttgat ggcaaaaagc tgatttgtta tggaggcagt cagtccatcg ctgaacccgg
     2521 aagctggtca gtggtaggga aaaagctgct caaagatgta ggcattcaca cccaacattt
     2581 ttatgattat tttgatcgta attactataa agatcgaaaa cttgggcgtg gcctgtattt
     2641 cagtcatgat caatacgaca aagatcacgt atcagataat atcttgggag ttgaaatagg
     2701 agataacgaa caagagatca ttgatattat taatgagtat ccgattgcga aagcctcaaa
     2761 ggttgctctg tttagattat tgagtgacaa gaagaattat ctaaaaggca tgggatctag
     2821 agaggataga at
//
LOCUS       DASCTH010000206         2520 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000338216, whole genome shotgun sequence.
ACCESSION   DASCTH010000206 DASCTH010000000
VERSION     DASCTH010000206.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000338216"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="QGH35_04400"
     CDS             <1..299
                     /locus_tag="QGH35_04400"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687800.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HJL81808.1"
                     /translation="KELEAIKQVFGENIPKISSTKSLTGHSLGVAGVHEAIFSLLMME
                     SDFISASANVEKPDPAAEGIPIVTSMQENAGLSTVMSNSFGFGGANASLVFSKL"
     gene            complement(337..939)
                     /locus_tag="QGH35_04405"
     CDS             complement(337..939)
                     /locus_tag="QGH35_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81809.1"
                     /translation="MSEKIHQYLLDVSKKHCKPLESIVKQNGVMHIVVPKDLDIFDCL
                     AKTVVEQQLSYKAANSIWNKIKNSAAKRNISLIEYFKKKNTASIRKNGLSSNKIKTIM
                     GAKKAIESGDITLQKLSIMTYPEFKKTITSLWGFGDWSAEMIAIFYFGKTNIWSPKDL
                     ILKRGIDQLCDDSNLTPNKLLQLVDPYQSYLALHIWRNKD"
     gene            993..1069
                     /locus_tag="QGH35_04410"
     tRNA            993..1069
                     /locus_tag="QGH35_04410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1027..1029,aa:Arg,seq:cct)
     gene            1096..>2520
                     /gene="murF"
                     /locus_tag="QGH35_04415"
     CDS             1096..>2520
                     /gene="murF"
                     /locus_tag="QGH35_04415"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HJL81810.1"
                     /translation="MFELIDVKDLKTLSTIIDGELIGENKPFTSLITDSRKVKKNSVF
                     LALQGEDFDGHNFCESAIHSGSIAYISNNKINNHDGIVVQDTYIALLKLAKFQQEKVK
                     PKTLAITGSNGKTTVKEMMAFIFKDQQNIVTTEKNDNNQFGVPFTVLRLKNNTKYLVL
                     ECGARKIGDFDLISEYLYFDVVAMTNVNNSHIGIFKNQQNIIKTKIKLLDGLVDRGSV
                     INGAFENLNSPTQFMKNDQGYKVTTHANSNSSEKPLKEEFLCRATADHTPGRYRLLIK
                     KIDGENSQSMEMQGIGTFGAVHNCHNALIAAVAATELGKGFSDSIAQICKYVSPLRDR
                     FFIEKMGKHLLIDDTYNANPSSFSSAINDLSNNSAYPKNKLLVIGDMLELGEFSREEH
                     LRILNESLKVPGLKALFIEGNRFKKALFERGHNREEEKIFVIDSQESFPIELISQFLD
                     EETTILVKGSRGLKMERIISLFVEN"
BASE COUNT          831 a          394 c          470 g          825 t
ORIGIN      
        1 caaaagaatt agaagccatt aagcaggtgt ttggtgaaaa catcccaaaa attagttcta
       61 ccaagtcact cacaggacat tccttgggag tagcgggggt ccatgaagct attttttcat
      121 tattaatgat ggaatctgat tttatttcag cctctgcaaa tgtagaaaaa cctgacccag
      181 ctgcagaagg gattccaatc gtcacttcga tgcaggaaaa tgcaggatta agtactgtta
      241 tgtctaatag ctttggtttt ggtggtgcta atgcaagttt agtttttagc aagctttaaa
      301 gctgaaaata tcgataactc tctaacacaa ggattttcag tccttatttc gccaaatatg
      361 tagcgctaaa taggattgat atggatctac taattgaagc agtttatttg gagttaagtt
      421 agagtcatca cataattgat ctattccacg ttttaagatt agatcttttg gagaccaaat
      481 gttggtcttt ccaaaataga agattgctat catttcagct gaccaatctc caaatcccca
      541 gagacttgta atagtttttt tgaattctgg ataagtcatg attgaaagtt tttgcaaggt
      601 tatatcgcca ctttcaatag cttttttagc acccataatt gtcttgatct tattgcttga
      661 taatccattc tttcttattg aagcagtatt ttttttcttg aaatattcaa tcaagcttat
      721 gtttcttttt gctgcactat tcttgatttt attccaaata ctatttgcag ctttataaga
      781 aagctgttgt tccactactg tttttgctaa acaatcaaag atatctaaat cttttggaac
      841 cacaatatgc attacaccat tttgcttgac tatagattct agaggtttgc aatgtttttt
      901 gctgacatcc aacaggtatt gatgaatttt ttcactcata gtttcacaat accaaaatga
      961 gcttacaatg attagtgcta taattttttt atgccccggt agctcagttg gatagagcat
     1021 cggattccta atccgggggt cagaggttcg aatcctcttc ggggcaccat ataaacttta
     1081 gtaaggaatg agtgattgtt tgaattaata gatgtcaagg atttaaaaac attgtcaaca
     1141 ataattgacg gtgagcttat aggagaaaat aagccgttta catctctaat aacagattcc
     1201 agaaaagtta aaaagaattc agtatttttg gccttacaag gagaagattt tgatggccat
     1261 aacttttgtg aatcagcaat tcattcagga tctatagctt atatatcaaa caataagatt
     1321 aataaccatg atggcatagt agtccaggat acttatattg cacttttgaa attagcaaaa
     1381 ttccagcaag aaaaagttaa gcccaaaacc cttgctatta ctggaagcaa cggcaaaact
     1441 actgtgaagg aaatgatggc atttatcttc aaagatcagc aaaacatagt gacaacggag
     1501 aagaatgaca acaaccaatt tggggtacca tttacagttt tgagacttaa aaacaatacc
     1561 aagtatcttg tattggaatg cggagctagg aagatcggcg attttgattt gataagtgaa
     1621 tatttgtatt ttgatgtggt agccatgact aacgtcaaca atagccatat tggtattttt
     1681 aagaaccagc aaaatattat taaaaccaag atcaagttat tagatggttt ggttgatcgt
     1741 ggctctgtta ttaatggtgc atttgagaat ttaaattctc caacacaatt catgaaaaat
     1801 gatcaaggat acaaggtaac tacacacgca aattctaatt catcagaaaa acctttgaag
     1861 gaagaatttt tatgtagagc gacagcagat cacactccag gtcgttatag attattgatt
     1921 aaaaagattg atggagaaaa ttctcagtct atggagatgc aaggaatagg tacctttggt
     1981 gcagttcata actgtcacaa tgctcttatt gctgcagtgg cagcaactga acttggtaaa
     2041 ggattttcag attcaattgc acaaatatgt aaatatgttt cacctttgag agatagattt
     2101 tttattgaga aaatgggtaa gcatcttctt attgatgata cttataatgc taatccttct
     2161 tctttttctt cagcaataaa tgatttgagt aataattcag catatccaaa aaataagttg
     2221 ttagtaatag gggatatgtt ggagttggga gaattttctc gagaggagca tctgagaatt
     2281 ctaaatgagt cattgaaagt tccaggactt aaagctttgt ttatagaagg caatcgtttc
     2341 aagaaagcac tatttgaacg tgggcataac cgtgaagagg aaaaaatctt tgtcattgat
     2401 tctcaagaat cattcccaat cgaactaatt tcccaatttc ttgatgaaga gacaacaata
     2461 cttgtaaaag gctctagagg gctgaaaatg gaacgaatta ttagtttgtt tgtagaaaat
//
LOCUS       DASCTH010000207         4736 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000338983, whole genome shotgun sequence.
ACCESSION   DASCTH010000207 DASCTH010000000
VERSION     DASCTH010000207.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000338983"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="QGH35_04420"
     CDS             complement(<1..578)
                     /locus_tag="QGH35_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81811.1"
                     /translation="MKKIILIITLLTPLHLQSDHHTIGGPGEGAFNALAVQAHDVTKY
                     VNLLKSDLSLFTALGATAGGVCITRSGHDYLGQMLVWSAFPDLASALHANTLYDPTSQ
                     APQALAKLRTIKYGTTWKGLKSFRLDPGWERVLKVKVSADNLNAYVESLRELETAIIN
                     DGHETFNLGVFGPFGGGVHESQTLMVRAISPTA"
     gene            722..1027
                     /locus_tag="QGH35_04425"
     CDS             722..1027
                     /locus_tag="QGH35_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HJL81812.1"
                     /translation="MKIQRVYSNQEEIKVEDRLFEAIKSLRSIDECRSFFQDICTPAE
                     LQAIKDRWSVADCLNQGYTYREINNLTGVSLTTIGRVARFLSDGSGGYQTALKRTSK"
     gene            1030..1926
                     /gene="hisG"
                     /locus_tag="QGH35_04430"
     CDS             1030..1926
                     /gene="hisG"
                     /locus_tag="QGH35_04430"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002866577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HJL81813.1"
                     /translation="MKEERIKIALQKSGRLTDHTLELLQKCGLVITKGKDKLIAYGEN
                     MPFDLLFVRDDDIPSLINDGLCHLGFVGLNVAMEKNIHFKSLSDNKPFSIEATLDFGQ
                     CRLSFAYPDEADIQSISDLEGKTVATSYPRIVSDYIDQQNLNINVTEFTGAVELAPSL
                     GKSDAICDLISSGSTLRAHNLIEGEVVLESSAVIISANNQLSESKQQWVDKLLERIDG
                     VLQVRESKYIMMHAPRDAIDKITALLPGSESPTIISLEGTDKMVALHMVCLEDVFWET
                     LEGLKSEGASSILVVPVEKMLA"
     gene            1907..3235
                     /gene="hisD"
                     /locus_tag="QGH35_04435"
     CDS             1907..3235
                     /gene="hisD"
                     /locus_tag="QGH35_04435"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019280850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HJL81814.1"
                     /translation="MRKCWPRMMDMLPLIQWNDLSEDERQQALQRPMISRSEGTLNTV
                     KAIIKKVSTKGDVALRELTLLLDQVELSDFKLSKAEIDRAIEKTSPTVKSAINQAINN
                     VRKFHEKKTKRLDISIEVMPGVSCEEITRPIETVGLYIPSGNNPLPSTAIMLGVPSLL
                     AGCPNRILCSPPNKEGDVDKNIVTAAVACGIVDIYKLGGAQAITAMAYGTESIPRVDK
                     IFGPGNTWVTVAKSLVAAANIGVSVDMPAGPTEVMVISNGGINSTSIAYDLLSQAEHG
                     PESQVILLCTDADFAQQVQDDIFQILVDLPTKEIASQALQNSKIIMIETIEQALSIAN
                     FYAPEHLILAFTDARNYIDQVQNAGSVFLGNWSPESAGDYCSGTNHVLPTYGYAKSVS
                     AISVDSFIKSISVQELSKEGLISLSKTITQLARVEGLEAHARAVEVRLEN"
     gene            3237..4295
                     /gene="hisC"
                     /locus_tag="QGH35_04440"
     CDS             3237..4295
                     /gene="hisC"
                     /locus_tag="QGH35_04440"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HJL81815.1"
                     /translation="MSTEIKNLIRPEILQLRPYQAAQYQPEMVRMNANENPYSLYEDK
                     DVSSLNLYPEAVPTKLRTSIANYLNVNVDNCVVTRGSTEAIDLLIRVFCRPQYDEIVI
                     FPPTFEMYQFYAEVQGVKIRTIPLLANQKFTLDLETFQKQVSPVSKLTFLCSPSNPTG
                     NTFDQSITFKTCKTLEDLGILVFDGAYTEFANIDYTSELLNEFSNVVVLRTLSKAFAL
                     AGSRCGVLLAAKEICDYVMRVIPPYSFSVPAGQTVLKGLSKQGIKSSKENVEKIIHQR
                     TYLMGKLSELSCVKKVFPSEANFILIKVEDAEAFYNRAKDAGLLVRNVSYQPSLNNCI
                     RVSIGTREQNQQLLEAVQ"
     gene            4297..>4736
                     /gene="hisB"
                     /locus_tag="QGH35_04445"
     CDS             4297..>4736
                     /gene="hisB"
                     /locus_tag="QGH35_04445"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HJL81816.1"
                     /translation="MNSNNQKIIFLDRDGTIIFEPEDFQVDAIAKVQLVPGVILSLSR
                     LIDYGYALVMVTNQDGLGSNSFPETQFTEAHEYILDLFKSQGIEFKDVFICPHFETDN
                     CDCRKPATGLITGFLAQNNIDPQRSYVIGDRDTDMEFSKRLKLQG"
BASE COUNT         1534 a          835 c          950 g         1417 t
ORIGIN      
        1 gctgtgggtg aaatggctct taccatgagt gtttgtgatt catgaacccc tcctccaaaa
       61 ggaccaaata cacctaaatt gaaagtttca tgtccatcat tgatgatagc tgtttcgagt
      121 tctcttaatg attcaacata agcattaaga ttgtcagctg aaactttaac tttcaatact
      181 ctttcccaac cggggtccaa cctaaatgat tttaaacctt tccaagtagt tccatatttt
      241 atagtcctaa gtttcgctaa agcttgagga gcttgactag taggatcata gagtgtgttt
      301 gcatgtagag cagatgctaa atcaggaaat gcagaccaaa caagcatctg acctaaataa
      361 tcatgtcctg atcttgttat acaaacaccc cctgccgtag ctcccaaagc agtgaataac
      421 gacagatctg attttagtaa attaacatat ttagtgacat catgagcttg aaccgctaat
      481 gcattaaagg ctccttctcc aggaccgcca attgtatgat gatcagattg gagatggagt
      541 ggtgtaagta aagttattat aagaatgatt tttttcataa tttttcccta ttttttaatt
      601 tttcacaacc atagcactaa aatatctttt ttacttaaat actcttttaa tttctagtct
      661 tgattaaaag aaataattaa tataatgtat tagtatatta atacactaat atataaatac
      721 tatgaaaata caaagagttt attctaatca agaagaaata aaagttgaag acagattatt
      781 tgaagctatt aaatcattga gaagtattga tgaatgtcgt tctttttttc aagatatttg
      841 cacccctgct gaacttcagg ctatcaagga tcgttggtct gttgctgatt gcctaaatca
      901 aggatacacc tatcgagaga tcaataattt aacaggagtc agtttaacca ctattggtcg
      961 tgtggctaga tttctatccg atgggtcagg tggatatcag actgcactta aacgaacatc
     1021 taaataaaga tgaaagaaga aagaataaaa atagcattac agaaatctgg aagattgaca
     1081 gaccataccc ttgagctgtt gcaaaagtgt ggattggtaa tcactaaagg caaagataag
     1141 ctgattgctt atggtgaaaa tatgccgttt gacctcttat ttgtgagaga tgatgatata
     1201 ccaagtttaa tcaatgatgg attatgtcat cttggttttg tgggtcttaa tgttgccatg
     1261 gagaagaata tacattttaa atccctttca gataataaac ccttttccat agaagcaacc
     1321 cttgattttg gtcagtgcag attatcattc gcatacccag atgaagctga tatacagtcc
     1381 attagtgatc ttgaaggaaa aacagtggca acgagttatc caagaattgt cagtgattat
     1441 attgatcaac agaatctgaa tattaatgta accgagttta ctggtgcggt tgaacttgct
     1501 cctagtctcg gaaaatcaga tgcaatctgt gatttaattt cttctggatc aacgttacgg
     1561 gctcacaatc taatcgaagg tgaagtagtt ttagagagtt ctgcagttat aatttcagct
     1621 aataatcagc tcagtgaatc taaacaacaa tgggtggata agttattaga gagaattgat
     1681 ggcgtgctac aggttcgaga aagcaaatac atcatgatgc atgcaccgag agatgcaata
     1741 gataaaatta ctgccctgct tcctggcagt gaatctccaa ctatcatttc gcttgaaggg
     1801 acggacaaaa tggtggcttt gcatatggtt tgtttggagg acgtattttg ggaaacactg
     1861 gagggtttaa agagtgaagg cgcttcatcc atcctagtag tgcctgttga gaaaatgctg
     1921 gcctagaatg atggatatgc tgcctttaat ccaatggaat gatctttctg aagacgaaag
     1981 acaacaggct ttgcaacgcc caatgatctc tcgatcagag gggaccttga atacggttaa
     2041 agcaatcatt aagaaagtta gcaccaaggg agatgttgca ttgagagagc taactttatt
     2101 attagaccag gtcgagttgt cggattttaa gctgagcaaa gcagaaatag atagagcaat
     2161 tgaaaagaca tcacccacag taaaatctgc aattaaccaa gccataaata atgtaagaaa
     2221 attccatgag aagaaaacaa aacgtttgga tatcagtatc gaagttatgc ctggtgttag
     2281 ctgtgaagaa attactagac cgattgagac cgtcggttta tatatacctt ctggaaacaa
     2341 cccgctgcca tcaacagcga tcatgttagg tgtaccttct cttttagcag gatgtcctaa
     2401 tagaatatta tgctcccctc ctaataaaga aggtgatgtt gataaaaata tcgttaccgc
     2461 agcagttgcg tgcggtattg tcgatatcta taagttaggt ggggcacagg ctattactgc
     2521 tatggcttat ggaactgaat ctataccaag ggtagataaa atatttggtc caggaaatac
     2581 ttgggtgaca gttgcaaaat cattggttgc agctgccaat attggtgtat cagttgatat
     2641 gcctgcagga ccaactgaag tgatggtgat aagtaatggt ggtattaaca gcacgtcaat
     2701 tgcttatgac ttgctctctc aagctgaaca cggtccagag tcgcaggtaa tcctattgtg
     2761 cacagatgct gattttgcac agcaagtgca agacgatatt ttccaaatat tagttgattt
     2821 acccactaag gaaattgcct cacaagctct acagaatagt aaaattatta tgattgagac
     2881 tattgaacaa gctttgtcga ttgctaattt ctacgcaccg gagcacttaa ttctggcatt
     2941 tactgatgcc agaaattata tcgatcaagt tcaaaacgca gggtctgttt ttcttggcaa
     3001 ctggagtcca gaatcagcgg gggattattg tagtggtacc aatcatgtgc tccctactta
     3061 tggttacgct aaatcggtca gtgccatctc ggttgatagt tttattaagt ccatttccgt
     3121 tcaagaatta agcaaagaag gcttaattag tctatcaaaa acgatcacac aattagctag
     3181 agttgaagga ttggaagccc acgccagagc agttgaagtt agattggaga attgatatga
     3241 gcactgagat aaaaaatcta attaggcctg agattcttca attaaggccc taccaagctg
     3301 cacaatacca acctgaaatg gtacggatga atgccaatga gaatccatac tctctctatg
     3361 aagataagga cgtttcttcg cttaatctat atccagaggc tgtaccgaca aaattgagaa
     3421 ctagtattgc caattacctt aatgttaatg ttgacaattg tgttgtaact cgtggcagta
     3481 cggaagcgat agatttactt atccgtgtat tttgtaggcc ccaatacgat gagatagtta
     3541 tttttccccc aacgtttgaa atgtatcagt tctatgcaga agttcaagga gtaaagataa
     3601 gaactatacc tttacttgca aatcagaaat ttacacttga tctagaaaca ttccagaaac
     3661 aggtaagccc agtgagcaaa ttaacctttc tttgttcacc ttcaaacccc actggaaata
     3721 catttgacca atcaataact tttaagacat gtaagacact agaagacctt gggatactag
     3781 tatttgatgg tgcttatact gaatttgcca atatagatta cactagtgaa ttgttgaatg
     3841 aattcagcaa tgttgtcgtt ttacgcacat tatcaaaagc atttgctcta gccggatctc
     3901 gatgtggcgt cctacttgct gcaaaagaaa tttgtgatta tgtcatgaga gtcattccgc
     3961 cctactcttt ttcagtacct gctggacaaa ccgtattaaa gggattgagt aaacaaggca
     4021 ttaaatcttc aaaagaaaat gtggaaaaaa tcattcatca gcgaacatac ttaatgggaa
     4081 agttatcgga actaagctgt gtaaaaaaag tttttccatc tgaggcaaat ttcattttga
     4141 ttaaggttga agacgcagaa gcattctaca atagagcaaa ggatgcaggt cttctagttc
     4201 ggaacgtaag ttatcaacct agtttaaata actgtattag agtctctatc ggcacaagag
     4261 aacaaaacca acaattactc gaagcagttc aatgagatga attcgaataa tcaaaaaatt
     4321 atatttctag atagagacgg gacaataatc tttgaacccg aagacttcca agtcgatgct
     4381 attgctaaag ttcaattggt acctggggtg attctttcat tatcaagatt gatcgactat
     4441 ggatatgccc tagttatggt tacaaatcaa gatggactgg gttcaaatag ttttcctgaa
     4501 actcaattta ctgaggctca tgagtatata ctggatttgt ttaaatccca gggcattgag
     4561 ttcaaagatg tttttatttg tccccatttt gaaaccgata actgcgactg cagaaaacca
     4621 gcaacgggtc ttattacggg gtttttagca caaaacaaca ttgatcctca aagatcctat
     4681 gttattggtg atcgtgacac cgatatggaa ttctccaagc gacttaaact acaagg
//
LOCUS       DASCTH010000208         5646 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000339560, whole genome shotgun sequence.
ACCESSION   DASCTH010000208 DASCTH010000000
VERSION     DASCTH010000208.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000339560"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(25..759)
                     /locus_tag="QGH35_04450"
     CDS             complement(25..759)
                     /locus_tag="QGH35_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HJL81817.1"
                     /translation="MIKEAVILAAGMGTRLASTLSTYPKGFITFDQFPIIEESVKKLL
                     KIGIEDIWVVTGYEPQYFERLAEKYPKNIHTIWNAEFEESGTMHSLYCVKESLKGSFL
                     LLESDIIYESRALEIITTHPSDHCILLSDFSYSGDEVFVETKNNFLINMSKNKSQLQN
                     KPTGELVGITKISQRLLNCMTTHAEEKFEHTLFYDYETGALVDSSKDIDIDCLLVEDL
                     IWAEIDDPDHLKRAINLIYPMIKSNE"
     gene            complement(759..1862)
                     /locus_tag="QGH35_04455"
     CDS             complement(759..1862)
                     /locus_tag="QGH35_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJL81818.1"
                     /translation="MSKLSGEILLNPGPVNLSQKVRVALSNKDLCHREKEFGELIKDI
                     NKRLGQVYPELSKEDYQSVLLTSSGTGAVEAMLKSFIPKNSLTLVINNGVYGARMEHI
                     LKLSNRPYKTLQFNWEEAISIDQIEQALREFPEIKNLALVHHETTTGRLNPLMAIGEV
                     CKQHGVGIFLDAVSSFGAEKIIGESINLSALAATAGKCLHGAPGISLVMAHPDLWSNP
                     VQEVDSVYFDLSDYYKSQYISGFSPFTQATHVAMAFQIALEEFFEAGGWEQRNELYKI
                     RADQIADSLVALGIKTLLPVSDFSSVLYSYLLPSGVSYHELHDYLKNKGFVIYQGQGK
                     LQDMIFRISTMGEISMDDIHDLSDNFIQFFDSN"
     gene            complement(1863..3008)
                     /gene="aepY"
                     /locus_tag="QGH35_04460"
     CDS             complement(1863..3008)
                     /gene="aepY"
                     /locus_tag="QGH35_04460"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019496677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HJL81819.1"
                     /translation="MIDAESFIHKAQDLGFSSYTGVPCSYLKHFISCVISSPDIDYVP
                     VVNEGDGIAFAAGVYLGGQYPVVMLQNSGLGNAVNPITSLLQNFEIPILILVTLRGDP
                     ASDPDEPQHRLMGQITCDLLNLMGISWSWFPRENSEIPSVLEAITSSVINNGKSYALV
                     IKKNSVKPYPIEKGPVKSLRTPELIQKETDINSPYRREEVLRAIIENSREEDLIIATT
                     GYAGRELYAIADNSNHFYMVGSMGCAVSIGLGLAKAKPKMRVIVIDGDGALLMRLGAM
                     TSVGYENPDNLIHVLLDNGEYESTGAQKTVSNTVQFCEMAMAAGYSRVSNLNSLKEIR
                     KHILPQKQRLSFLYVPTQSGTIDNLPRQVITPPQVASRFKKHIQGIQ"
     gene            complement(3005..4639)
                     /gene="aepX"
                     /locus_tag="QGH35_04465"
     CDS             complement(3005..4639)
                     /gene="aepX"
                     /locus_tag="QGH35_04465"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HJL81820.1"
                     /translation="MGYTTIANKTSQFRSLLNGPKLEFLLEAHNGISAKIAEESGFKG
                     LWAGGLAIAAQYGVRDSNEASWTQVLEVLEFMSDATSIPIMLDGDTGYGNFNNVRRLV
                     QKLEQRGIAAVCIEDKLYPKTNSFIDGNKQELANIDEFCSRIKAGKDAQKDDEFSIIT
                     RVEAFIAGWGLDEAIKRAEAYRQAGSDGILIHSSKSEPSEIIAFKNEWGDRLPVIIVP
                     TKYYSTPTEIFSQVGISIAIWANHMIRSSIESMQQTAASIIQKKSVVALEDKIASVSE
                     IFRLQGVSELLEAEEMYLPKKQKATSAIILAASRGSAMGSLTEERPKAMLKVKERPIL
                     RHIVDVYNSHGIKNITVVRGFQKDAIDLPQLSYADNDNFSKTDELVSFEKALLEVDLV
                     QDLFVSFGDVLFKPYLLQLMDDFQDEVVIVVDTQWQDSVNKERAADYVHCSEPHSRDS
                     YSHEIFLKDSKEDMDPDMIHGEWMGVVKFSNKMIPTLKQAMQKLKMSSDFDLAKFHDL
                     FSFLVSEGHNIRIVYTTGHWLDIDTVEDLLEAGNFL"
     gene            complement(4650..>5646)
                     /locus_tag="QGH35_04470"
     CDS             complement(4650..>5646)
                     /locus_tag="QGH35_04470"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HJL81821.1"
                     /translation="QLWNASHPLGEEYSEEEQKKCLSIFAFWNPPGNDIGRQWAKAQE
                     MNQPLELAIACIVDPAVQVAAATSITFGEDEMDFAGGLRGEGVDVVQCETVDLAVPAT
                     AEWVIEGKFLPGRDIAIGPHSNPVGYYDDVQFLPLIEVDCITHRKDPIWYATMEMEPP
                     FDHNYMACMPLEGEVLSDLQTKIPEVLDVVVTPNLSYFVQLSVDGEQKPRADFGKEVL
                     QAVWGANGRWGPTAKLVVVVGPDVNPYDLSEIEWAIQTRVQPQTDTIINEAGQAFLMD
                     PSAVKDPGHGIALVSEQIGIDATIKIPERFESYPKYSNAEPKDVMAIAEKLKEVL"
BASE COUNT         1675 a         1207 c          993 g         1771 t
ORIGIN      
        1 aaaatggagt tattagtaac aatattattc atttgattta atcattggat aaattaagtt
       61 gatggccctt tttaaatggt caggatcatc aatttccgcc caaattaagt cctctactaa
      121 aagacaatcg atatctatgt cctttgatga atcaaccaat gcaccggttt catagtcgta
      181 aaataaagta tgctcaaatt tctcttctgc atgagtagtc atgcaattta ataaccgttg
      241 actaattttt gtgatgccta ctaattcacc agttggctta ttttgaagtt gagatttatt
      301 ttttgacata ttaattaaaa agttattctt tgtctcaaca aaaacttcat ccccagagta
      361 ggaaaaatca gaaagcaaaa tgcaatgatc agaaggatgg gtcgtaataa tttcaagagc
      421 tctactttcg taaataatgt ccgactctaa taacagaaat gagcctttta aggattcttt
      481 gacgcaatac aatgaatgca tggttccaga ttcttcaaat tctgcattcc aaatggtatg
      541 gatatttttt ggatattttt ctgccaatct ctcaaagtat tgtggttcgt aaccagttac
      601 aacccaaata tcttctatac ctatttttaa cagtttctta actgattctt caataattgg
      661 aaattgatca aaggttatga atcccttggg gtaagtagat aaagtactgg ctaaacgagt
      721 gcccattccg gcggccaaaa taactgcttc tttaatcatt aattgctatc aaaaaattgg
      781 ataaaattat cagaaagatc atgaatgtca tccatactaa tttctcccat tgtcgaaatt
      841 ctaaaaatca tatcttgtaa ctttccctgt ccttggtaaa taacaaaacc tttgtttttt
      901 agatagtcat gtaactcatg ataggaaacg cctgaaggca agagataaga ataaagtacc
      961 gatgaaaaat cagatactgg aagaagtgtt ttaatgccga gagccactaa agaatctgct
     1021 atttgatcag ctcgtatttt atacaactcg tttctttgct cccagccacc ggcttcaaaa
     1081 aactcctcta gagcaatctg aaaggccata gcaacatggg tagcttgtgt aaagggagag
     1141 aaaccactga tgtattgtga tttataataa tcagacaaat caaaataaac actatctact
     1201 tcttgcaccg gatttgacca tagatcagga tgggccatga ctaaggaaat tcctggtgct
     1261 ccatggaggc acttgcctgc cgttgcagca agtgcagaaa gattaatact ctcaccaata
     1321 attttttcag caccaaaact acttacagcg tctagaaaaa taccaacgcc gtgttgttta
     1381 caaacctcac caatagccat gagaggattt aatcgacctg ttgttgtttc atgatgaacc
     1441 aaagcaaggt tcttaatctc tggaaactct ctcaatgctt gctcaatctg atcaatactg
     1501 attgcttctt cccagttaaa ttggagggtt ttatagggac gattcgataa ctttaagatg
     1561 tgctccattc ttgccccgta aaccccattg ttaattacaa gtgtgagact gttttttgga
     1621 ataaaactct ttagcatcgc ctcaacagca cctgtcccgg atgaggttaa tagaaccgac
     1681 tgataatctt cctttgataa ttcaggataa acttggccta atcttttatt gatgtctttt
     1741 atcaattcac caaattcctt ctctctatgg caaagatctt tattagaaag agctacccgg
     1801 accttctgag ataaattcac tggaccgggg ttcaataata tttcaccaga taatttactc
     1861 atttattgaa tcccttgaat gtgtttttta aatcgagatg ccacttgagg tggggtaata
     1921 acttgtcttg gtaaattgtc tatggtgcct gattgggttg gcacatataa gaatgataat
     1981 cgctgtttct ggggcagtat atgctttcta atctccttta atgaattgag attactgact
     2041 cgtgaatagc cggcagccat tgccatttca caaaattgaa cggtgtttga gactgttttt
     2101 tgggcccctg tagactcgta ttcaccatta tctaggagaa catggattaa attatcagga
     2161 ttttcatacc ccacactagt catagcacct aaacgcatga gtaatgcgcc atctccgtca
     2221 ataacaataa ccctcatctt gggttttgct tttgccaatc ccaaaccgat actaacagca
     2281 catcccattg aaccaaccat ataaaaatga tttgagttat cagcaattgc atataactct
     2341 cttccagcgt aaccggttgt tgcaatgatt aaatcttctt ccctgctatt ttcaataatg
     2401 gctcttaaca cttcttcccg gcgatacggg gaattaatat ctgtttcctt ttggattagt
     2461 tcaggtgttc ttaatgattt tactggtccc ttttctattg ggtagggctt gactgaattt
     2521 tttttaataa ccaatgcata ggattttcca ttgttgatga ctgaggaagt gattgcttcc
     2581 agcactgatg gtatttctga attctctctt ggaaaccaag accatgaaat acccattaga
     2641 ttgagtaggt cacaagtgat ttgtcccatc aaacggtgtt gtggctcatc cggatcagaa
     2701 gcaggatccc ctcttaaggt aactaagatc aaaatcggaa tctcaaaatt ttgtaataaa
     2761 gaggtaatcg gattaacggc attgcccaat cctgagttct gcaacataac aacagggtat
     2821 tgacccccta aataaactcc ggcagcaaag gcaattccat ctccctcatt gacaacagga
     2881 acataatcaa tatcaggaga actaatgaca caacttatga aatgttttag gtaagaacag
     2941 ggaacaccag tgtatgaact aaaacctaaa tcttgcgcct tgtgaataaa agattccgca
     3001 tcaatcatag aaaattccca gcttcaagaa gatcttcgac cgtatcaata tctaaccagt
     3061 gacctgttgt gtacacaatt ctaatgttgt gcccctctga aaccaaaaag gagaaaagat
     3121 catgaaattt tgctaggtca aaatctgaac tcatttttag cttctgcatt gcttgtttga
     3181 gtgttggtat catcttgttg gagaatttaa ctacgcccat ccactcacca tggatcatat
     3241 cagggtccat atcttccttt gaatctttga gaaatatctc atggctataa gaatcccttg
     3301 aatgaggttc tgaacaatgc acataatcag cagctctttc tttatttaca gaatcttgcc
     3361 attgggtatc cacaacaata acaacctcat cttggaaatc atccataagc tgtaataaat
     3421 atggtttaaa caaaacatcc ccaaaagaaa caaacaaatc ttgcacgaga tcaacttcca
     3481 aaagcgcctt ctcaaaagaa actaattcat ctgtcttcga aaagttatca ttgtcagcat
     3541 aactaagctg gggtaagtct atagcatctt tttggaatcc tctgacaaca gtaatgttct
     3601 taatgccgtg actgttatac acatcaacaa tatgtctgag aattggtctt tctttcacct
     3661 tcaacattgc cttaggtcgc tcttcagtca agctacccat agcagatcca cgcgatgcag
     3721 ctaagataat tgctgatgtt gccttctgct ttttaggaag atacatttct tctgcttcca
     3781 gcaattcaga aacgccttgt aatctaaaaa tctcactaac actagcaatt ttatcttcca
     3841 aggccactac tgattttttt tgaataattg atgcagccgt ttgctgcatt gattcaattg
     3901 aactacgaat catatgattt gcccagatag caattgaaat acctacttga gaaaatatct
     3961 ccgtgggagt ggaatagtat ttagtaggta ctatgataac tggcaagcgg tcgccccact
     4021 catttttaaa ggcgatgatt tctgaaggtt cagattttga gctgtgaatc agtattccgt
     4081 ctgagcccgc ttgacggtaa gcttcagctc tttttattgc ttcatctaag ccccatcctg
     4141 cgatgaacgc ctccactctg gtgatgattg aaaattcatc atctttttgg gcatctttac
     4201 cagctttaat tcgactgcaa aactcatcaa tattagccaa ctcttgtttg ttgccatcga
     4261 tgaagctgtt agttttagga tacagcttat cttctatgca taccgctgca attccccgtt
     4321 gctcaagttt ttgaactaag cgtctgacat tattaaagtt tccataaccg gtgtctccat
     4381 cgagcattat gggtatggag gttgcatctg acataaattc aagaacttcc aagacttgcg
     4441 tccagctggc ttcattgctg tcacgaaccc catattgtgc tgcaatggct aaaccacctg
     4501 cccacaatcc tttaaatcct gattcctcgg cgatttttgc actgatccca ttatgcgcct
     4561 caagcaaaaa ttctagtttc ggaccattga gtagtgatct aaattgactg gttttattag
     4621 cgatagtcgt ataccccaaa caataattat tacaatacct ctttcaattt ctctgcaata
     4681 gccatgacat ccttaggttc agcattgcta tatttgggat atgattcaaa acgttctggt
     4741 atttttattg ttgcatcaat gccaatttgt tcggatacca gtgctatccc atggcctgga
     4801 tccttgactg cagatggatc cattaaaaaa gcttgtcccg cttcgttaat gatagtatct
     4861 gtctgaggtt gaaccctggt ttgaatggcc cattctattt ctgatagatc ataaggatta
     4921 acatcaggtc ccacgacaac aactaatttt gctgtaggcc cccatcttcc gttagcgccc
     4981 caaacagctt gcagaacctc cttaccaaaa tcggcacgtg gtttttgttc cccatctaca
     5041 gagagttgaa caaaataact aagatttgga gtcaccacga catctaggac ttcaggaatt
     5101 tttgtttgca gatcactcag cacttctcct tccagtggca tgcaagccat atagttgtga
     5161 tcaaatggtg gttccatttc catggttgca taccaaattg gatctttgcg atgagtgata
     5221 caatcgactt caatcagtgg caagaactgg acatcatcat aatagccaac agggttagaa
     5281 tgcggtccta tcgcaatatc acgtccaggc aaaaattttc cctcaatcac ccattctgct
     5341 gtagctggga ctgcaagatc tactgtttcg cactgcacca catcaacacc ttcacctctc
     5401 agtcctcctg caaaatccat ttcgtcctca ccaaaagtaa tagaagttgc cgcagcaact
     5461 tggactgcag ggtctacaat acaagcaata gcaagttcca gcggttgatt catttcttgt
     5521 gcctttgccc actggcgtcc aatatcattt cctggaggat tccagaaagc aaaaattgat
     5581 agacattttt tctgttcttc ctctgaatac tcctcaccga gtggatgaga tgcattccat
     5641 agttgg
//
LOCUS       DASCTH010000209         2611 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000340700, whole genome shotgun sequence.
ACCESSION   DASCTH010000209 DASCTH010000000
VERSION     DASCTH010000209.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000340700"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="QGH35_04475"
     CDS             complement(<1..1106)
                     /locus_tag="QGH35_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HJL81822.1"
                     /translation="MSQHAFKNKGRIRVNNKIIAAFIVTLTYFLFYLFTANAVLIQLQ
                     PMDATISVRGGGFPHFKIGGRYLLRKGNYRLNISHPGHYPLIETIEVNEDSSQNFVFD
                     LKRLPGKLFIRTDPESKITLKVDDTVFEPSQNGEFIISAGEHALEILTERYFAVEQNI
                     LIQGMEIQQEMEISLIPAWAEISIASLPMEANILINGQLIGTTPDTVEILEGEYVMLL
                     TKKGYKLFEQRLKVKASQSQVIDGIELSKLDSTLKVITKPTDAAININGIYRGLSPIV
                     IDLPPEKTHLLEISKPGYKSVSKEIILDTREAMESAQIDFIEFQRTLEPLMGSIKITG
                     TKKAAIFSDDKQIASIPASIQLLAKEQTLTVKKEG"
     gene            complement(1103..>2611)
                     /locus_tag="QGH35_04480"
     CDS             complement(1103..>2611)
                     /locus_tag="QGH35_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81823.1"
                     /translation="MDHKNSENGSEIKTDLSSNQAEKSAKTIHQQNLEKDDRNKVSRR
                     HAYSLAAISMVVLLIVVLVLPVVIQETDEPIQQIEDSQSQTDQLTIDREILAQKPIAQ
                     AMFSELLIKIDEIKLFGVQFWGGEQWEMVLKLQQEGDTNYQTAQYHLAANNYRDAMQH
                     LVGLEMSIPALLEESLDRGMEAILSGNKELAIKNYETALAIDGINQEAKKGLDRALKL
                     NRVLELTQLGNDFESKRQWKEAIQAFEEALAIDQEWQQAQSGFDRSKEQFETEEFQRL
                     LSLGYQAIRENRFELARSSFDQAVDIQRESPEIQQAFEELDTQERIFNIKSMKYKALT
                     AEVNEQWDNAQALYKSILNLDPNIGEIKESLTRVNQRIELSNNLTYFISSKDKLNDDK
                     FFTQAETMLTQAQNVINPGPVLKEQISEMAQILKIAAIPIEVTIFSDNATEILIFKIG
                     NLGVFERSVIPLRPGVYIATGKRNGYRDVQLRFKVSASKKDQTIRVQCKERI"
BASE COUNT          709 a          559 c          460 g          883 t
ORIGIN      
        1 ccctcttttt taacagtcag agtttgttct tttgctaata actgaatgct tgccggtatg
       61 gatgcgattt gcttatcatc ggaaaaaatt gctgcctttt ttgtaccagt gattttgata
      121 gaacccatta atggttcaag agttcgctga aactcgatga aatcaatttg agccgactcc
      181 attgcttctc ttgtatctag aataatttcc ttgctcacag atttatatcc gggtttagaa
      241 atttctaaga gatgggtttt ctcaggcggt aagtcgatca cgatgggtga caagccccga
      301 taaattccat taatattgat cgcagcatcg gtcggcttgg ttatcacttt gagtgtgcta
      361 tctaatttgg ataattcaat accatcaatc acttgcgatt gactggcctt aacctttaat
      421 ctttgttcaa agagtttata gccttttttc gtgagcagca ttacatactc cccttcaaga
      481 atctctacgg tgtcaggagt agtcccgatt aattgtccat taataagaat gttggcttcc
      541 ataggtaaag acgcaattga aatttctgcc caagcaggga tcaaactgat ttccatctcc
      601 tgctgtattt ccatcccctg aatgagaata ttctgttcta ctgcaaaata tctctccgtt
      661 agaatctcta aagcatgctc acctgcagaa ataataaatt ccccgttctg gcttggctca
      721 aaaacagtat catctacctt cagagttatt ttggactcag ggtcggttct gataaaaagt
      781 ttccctggca atcgttttaa gtcaaaaaca aaattttgac ttgaatcttc gttaacctct
      841 atggtttcaa tgagcggata atgacccgga tgtgaaatat ttaatcgata gttgcctttt
      901 ctcaataaat accgaccccc tattttaaaa tgaggaaacc cacctccacg aacgctgatt
      961 gttgcatcca tgggctgaag ttgtatcaaa acggcatttg ctgtaaacag atagaaaaga
     1021 aaataggtca aagtgacaat gaaagcagca ataatcttat tgttaactcg gattcgaccc
     1081 ttgttcttaa acgcgtgctg actcatatcc tctccttaca ctgcacgcga attgtttggt
     1141 cttttttgct agcagacact ttaaatctta gttgcacatc acgatacccg ttgcgtttac
     1201 cagttgctat ataaacaccc ggcctgagtg gaatcacaga tcgttcgaac acgccaagat
     1261 ttccaatctt aaaaataagg atttctgttg cattgtctga aaaaatggtg acttctatcg
     1321 gtattgccgc aatcttgaga atttgtgcca tctcactgat ctgttctttg aggactggcc
     1381 caggattaat aacgttctgc gcctgcgtca gcattgtttc agcctgagta aaaaatttat
     1441 catcgtttaa cttgtcttta ctgctgataa agtaggttag attatttgat aactcaatgc
     1501 gttgattcac cctagtaaga ctctctttga tctcgccaat atttggatcc aggtttaata
     1561 tgcttttata caatgcctgc gcattgtccc attgctcatt gacctcagca gttaaagctt
     1621 tgtatttcat cgacttgatg ttaaaaattc tttcctgagt atccaactcc tcgaaggctt
     1681 gttgaatctc aggagattct ctctgaatgt ctacagcttg atcaaacgat gatcgtgcta
     1741 attcaaaccg attctccctg atggcctgat aacctaaaga gagtaaacgt tgaaactctt
     1801 ctgtctcaaa ttgctcttta ctccgatcaa atccagactg tgcttgttgc cattcctgat
     1861 ctatggccaa ggcctcttca aaggcttgta tagcttcttt ccactgcctc tttgactcaa
     1921 aatcattacc caattgagtc aattccaaca cacgattaag cttaagcgcc cgatccaatc
     1981 ctttttttgc ttcttggttg atcccatcaa tggcaagagc tgtttcataa ttttttatcg
     2041 ctaattcttt atttcctgac aaaatcgctt ccatacctct atcaagagat tcttccaaaa
     2101 gagccggtat gctcatttct aatccaacca agtgttgcat agcatcacga taattattgg
     2161 cagcaagatg atattgagct gtttggtaat tggtatcacc ttcttgctga agttttagaa
     2221 ccatttccca ttgttcccct ccccaaaatt gaacaccaaa aagttttatt tcatcaattt
     2281 ttatcaataa ttcggaaaac atggcttgag caataggttt ctgtgccaaa atttcacggt
     2341 ctattgttaa ctgatctgtt tgggattgag aatcttctat ctgttggatt ggttcatcgg
     2401 tttcttgaat tacaactggc agaaccaaaa caacaattaa taaaaccacc attgagatag
     2461 ctgccagaga ataggcatgc ctcctactga ccttatttct gtcatctttt tcaagatttt
     2521 gttgatgaat agtttttgct gatttttcag cttgattact gcttaaatct gttttgattt
     2581 ctgagccgtt ttcagagttc ttgtgatcca t
//
LOCUS       DASCTH010000210         1845 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000348361, whole genome shotgun sequence.
ACCESSION   DASCTH010000210 DASCTH010000000
VERSION     DASCTH010000210.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000348361"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="QGH35_04485"
     CDS             complement(<1..570)
                     /locus_tag="QGH35_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012575.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HJL81824.1"
                     /translation="MYINFWYPIVIGEELKAGKPEKVKVLGVDVVAFRDSKGNANVIS
                     DTCCHRGGSLGGAWSNLPTAPRIIEDSIVCPYHGWEFGGDGKCTNIPSLGYGAKVPDR
                     AKVDAYPVQEKYGIVFAFLGDLPEEERPPLLDIEEYGTDEWRTNKVLAFEVPFNFERS
                     VENGLDPAHNEFVHPTHGFTGINRDTYIVN"
     gene            complement(627..1313)
                     /locus_tag="QGH35_04490"
     CDS             complement(627..1313)
                     /locus_tag="QGH35_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015857.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HJL81825.1"
                     /translation="MKAENVLLAFVVAFLLGFQAVAIAQEQTSSGIQSGDWIVRAGMV
                     NVDPQSNNGDIVTVSDDTRLSINFTYMVTDNIGLDVLGALPFEHDLYTTPTVETALGI
                     PTGTWAASTEHLPPTVSAIYHFSPGSNFRPYVGVGLNYTKFFHESTKGGVLGDLGLKP
                     KIDSTTGLSFQIGFDFDVSDTLILNVDYRKIDIEPDAELFTESTGVTFLAFDAPLNPS
                     VFMVQLAMKF"
     gene            complement(1531..>1845)
                     /locus_tag="QGH35_04495"
     CDS             complement(1531..>1845)
                     /locus_tag="QGH35_04495"
                     /inference="COORDINATES: protein motif:HMM:NF024934.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJL81826.1"
                     /translation="FLLGAKNAIGKTATFLLFLKIRQRQYLTDPDYKEIPLTRADIAD
                     FLGLTIETVSRTITKFKQLKLIETVDQGRVKILDTHRLAEIADLEVAWDRWEINHKKK
                     NR"
BASE COUNT          540 a          391 c          353 g          561 t
ORIGIN      
        1 attgactata taagtatctc tgttgatccc tgtaaatcca tgggtaggat gaacaaactc
       61 gttgtgagca ggatcaagac cattttctac ggaacgttcg aaattaaagg gaacttcaaa
      121 agctagaact ttattggtcc gccattcatc tgttccatat tcctcaatgt caagaagagg
      181 tggtcgttct tcttctggaa ggtcgcctaa gaaagcgaag acgataccat acttttcctg
      241 cactggatat gcgtctacct tggcgcgatc aggtactttc gcaccatatc ctaatgaagg
      301 tatgtttgta catttaccgt ctccgccgaa ttcccaaccg tggtagggac aaacaattga
      361 atcctcgatg attctagggg cagttggaag atttgaccaa gctccaccta gagaaccgcc
      421 tcggtgacag caagtgtcgc ttatgacatt tgcattacct ttgctatctc taaaggcaac
      481 aacgtcaaca ccgagaactt tgactttctc gggcttccca gccttgagtt cttctccaat
      541 aacgattgga taccaaaaat taatatacat tttattttcc tcctaaatta ctactattgt
      601 tattattttc attcatattt attgaattaa aatttcattg ccaattggac catgaacact
      661 gacggattga gcggtgcatc gaatgctaga aaggttactc ccgttgactc agtaaataat
      721 tctgcgtctg gctcaatgtc tatttttcga taatcaacat ttaaaattag ggtgtcactt
      781 acatcaaaat caaaaccaat ctgaaaacta aggcctgtgg ttgagtcaat ctttggtttt
      841 aaaccaaggt cacccaatac tccaccttta gtgctttcgt gaaaaaattt tgtgtaatta
      901 agtcctaccc caacgtatgg cctgaaattg ctaccaggac taaaatggta aatcgcacta
      961 accgtcggcg gcaaatgctc tgtactggct gcccaggttc ctgttggaat acccaaagct
     1021 gtttctaccg ttggtgtcgt atataaatcg tgttcaaacg gcaaagcacc caagacatca
     1081 aggcctatgt tatctgtaac catgtaggta aagttgatcg ataaacgagt atcatcgctg
     1141 accgtaacaa tatcaccgtt gttggactga ggatcaacat tgaccatccc tgctctaaca
     1201 atccaatctc cagattggat accagaactg gtttgttctt gggcaatagc gacagcttga
     1261 aagcctagta agaaagcgac aacaaatgcc aacaagacat tttctgcttt cattattttt
     1321 ctcattacca taactcctaa ttggttaatg gaactaatta ttattcctgt gaaaaaaata
     1381 atcattgatc tgagacaagg gcttcttgat ttaaatcaga gggtgtttga tttaaatcaa
     1441 gtatccgaca gacatcattt caattgcgct gcattcgcaa cagaagttaa ttaaaaagaa
     1501 atgattaggg aagggaaaaa actgggctga ttagcgattt tttttcttgt gattaatctc
     1561 ccatcgatcc caagccactt ccaaatcggc aatttcggcc agccgatggg tatccaaaat
     1621 cttaactcga ccttggtcaa cagtttcgat cagttttaat tgtttaaact tggtgattgt
     1681 tctggaaacg gtttcaatgg ttagtcccag aaaatctgcg atatcggctc tagtcagtgg
     1741 gatttctttg taatcaggat ccgtcagata ttgccgttgt cttatcttca aaaagagcag
     1801 aaatgttgcc gttttgccta tggcattctt tgctccaagt aaaaa
//
LOCUS       DASCTH010000211         6520 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000348393, whole genome shotgun sequence.
ACCESSION   DASCTH010000211 DASCTH010000000
VERSION     DASCTH010000211.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000348393"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /gene="tilS"
                     /locus_tag="QGH35_04500"
     CDS             <1..1349
                     /gene="tilS"
                     /locus_tag="QGH35_04500"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HJL81827.1"
                     /translation="CRKNIDLGGTLINHIQRLNLTKIWVALSGGLDSIVLLHILHQIA
                     REQIYLRAIHFNHNLTEGSQSWAQFCKKICNQLNVNLDIYSLKINLHEGFGIEAAARK
                     ARYQKLGESIHKDECLMTAHHQDDQLETILLRMARGTGIDGLQAIQRQFDFGQGIILR
                     PLLDFTRDQIEDYAKRNKLEWIEDTSNKEVYFDRNFLRQKVIPIMKERWPSLPSSVSR
                     LSKISSQTLILLKELAEQDLGKDSSITELNIDVFNDKSNERVINMMRYLISRNKMSSP
                     SMRVLNAGINILLNDESINPSMFWDDCCIKRYRNKLYFLHASQLKPNQNLEPENWKIK
                     NPLRLDNDGGLLEVTLVRGQGLSLSKCDQNLTIRYRIGGESIKPVGHKITKKLKNLFQ
                     ENHILPWVRDKIPLIYSGTELIGVADLWIHDDYTASQNEQGFLIDWQRKINIKHNC"
     gene            1470..3101
                     /locus_tag="QGH35_04505"
     CDS             1470..3101
                     /locus_tag="QGH35_04505"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003632294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HJL81828.1"
                     /translation="MSHYIFVTGGVVSALGKGVTSASLGAILEARGLKVGMMKLDPYL
                     NIDPGTMSPYQHGEVFVTEDGTETDLDLGHYERYVNTTTGKNSNFTAGKIYENVIAKE
                     RKGDYLGGTVQVIPHVTDEIKKNITIGADGFDVTIIEVGGTVGDIESLPFLEAIRQMG
                     IEENGFKVAYVHLTLVPYIKTSSEIKTKPTQHSVKELRSIGIQPDALVCRSEKPLPEE
                     QRKKIALFTNVKEEAVISAQDASDIYEIPLILRDQGLDEIIINKLQLNVTKPDLTDWE
                     SVLLAKNSSKSLVKIAMVGKYVDFRDSYISLSEALRHAGFKTQTRIQIEYVESEEIQK
                     NGTGILKAMDAILIPGGFGDRGIEGMVDSIQFARENKVPFLGICLGLQAAVIEFSRNN
                     LNLLDANSTEFDPDTHAPVIALIKEWMNEDGSLENRDEDSDLGGSMRLGAQECMITNK
                     TLARDVYQKSSVFERHRHRYEFNINFKEDLEKKGLIFSGHSKDGLVEIIELADHPWFF
                     ACQFHPEFTSSPRKGHPLFESFIQASVEYSRTKKE"
     gene            3105..3917
                     /gene="kdsA"
                     /locus_tag="QGH35_04510"
     CDS             3105..3917
                     /gene="kdsA"
                     /locus_tag="QGH35_04510"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010958364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HJL81829.1"
                     /translation="MSVSIGKNKVGNNLPLTLIAGPCVIESLELAQEVAGSIAEIATK
                     LEIPFIYKSSFDKANRSSLSSYRGPGIDEGLKILESVKKSVDVPVLTDVHEDTPLEEI
                     ASIVDVLQTPAFLCRQTNFILSVAAQNKPINIKKGQFLSPWEMQNVVNKAKSVGNNNV
                     LVCERGFAFGYNNLVADMRSLVILRETKCPVIFDATHSVQLPGGQGSSSGGQKEFISS
                     LARAAVATGVAGIFIETHPDPEKALSDGPNSLPLNQLSSLLHALKDIDSIVK"
     gene            3972..5258
                     /gene="eno"
                     /locus_tag="QGH35_04515"
     CDS             3972..5258
                     /gene="eno"
                     /locus_tag="QGH35_04515"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HJL81830.1"
                     /translation="MSEIKSVQGRQILDSRGNPTVEAEITLHNGICARSSVPSGASTG
                     SREAVELRDGDPSRFFGKGVSIAVKNINTEIQKALIGQSVLEQKKIDKTLIELDSTED
                     KSRLGANAILAVSLAAAHTAASFSGKKLFQYLQSDDYSLPVPLMNIINGGEHADNNID
                     IQEFMILPVGAESYSEALQYGVEVFHSLKSVLKKQGLNTAVGDEGGFAPNLPSNIAAL
                     DTILQAIELAGFQPGGDIYLGLDVASSEFFDNEKYHLSSEGQSFDSIGFVDYLSKISK
                     DYPIISIEDGMSENDWAGWQALTQSIGNDIQLVGDDLFVTNTRIFQEGIDKGIANSIL
                     IKPNQIGTLTETFQAIDMAKSSNYTAIISHRSGETADTTIADIAVSTSVSQIKTGSLC
                     RSDRVEKYNQLLRIEEALAQEAKYLGKNAFGSIFNR"
     gene            5274..5576
                     /gene="ftsB"
                     /locus_tag="QGH35_04520"
     CDS             5274..5576
                     /gene="ftsB"
                     /locus_tag="QGH35_04520"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HJL81831.1"
                     /translation="MKFITIILIFLLLLLQINIWFKDDGYPRITELKSLIQDQKNENS
                     AMATRNTQLREEVEDLKKGDAAIEERARTDIGMIKEGEEFYLITQPKPILEETNSD"
     gene            5607..6395
                     /gene="surE"
                     /locus_tag="QGH35_04525"
     CDS             5607..6395
                     /gene="surE"
                     /locus_tag="QGH35_04525"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HJL81832.1"
                     /translation="MEILLSNDDGYYSKGIRELEKVLSDRATITMVAPETNCSGASSS
                     LTLKNPLRIRKFQSNGYYVNGTPSDCVYLALTSIVESKPDLVISGINYGQNLGDDVLY
                     SGTVGAAMGGRSLGVPCLAISITSLKPQYLETALTVTKLLVDNYINHRSLPNQVYNIN
                     IPDIPYEEVQEPLLTRLGKRDTQEAITRPDKDRYGDTIYWVGGPPPPKDASEGTDFFA
                     VQNNHVSITPLVSDFTDYQSLSAKHRSKDGSVQGWIAHLSNELF"
BASE COUNT         2188 a         1049 c         1356 g         1927 t
ORIGIN      
        1 gttgtaggaa aaatatagat ctcggaggga ccttaataaa tcatattcaa agactcaatt
       61 taacaaaaat ctgggtggca ttgagtggtg gattagattc cattgttttg ctgcatatcc
      121 tccatcaaat agccagagaa cagatttatt taagggcaat tcattttaat cataatttaa
      181 cagaaggttc tcagagctgg gctcaatttt gtaaaaagat ttgtaaccaa ctcaatgtta
      241 atttagacat ttattcttta aaaataaatt tacatgaagg atttggaatt gaagcagctg
      301 caagaaaagc tagatatcaa aaattaggag aaagtatcca caaggatgag tgcttaatga
      361 cagcacatca ccaagatgat cagctagaaa ctattttatt aaggatggcc agaggcactg
      421 gaattgatgg tcttcaagct attcagagac aatttgattt tggacaagga atcattttaa
      481 gacctctttt agattttacc agagatcaga tagaagatta tgcaaagcga aataaattgg
      541 aatggattga agatactagt aataaagaag tttactttga tagaaatttt ttaagacaaa
      601 aagtcattcc gatcatgaag gaaagatggc ctagtcttcc tagcagtgtt tctagattat
      661 caaaaatctc cagtcaaact ttgattttat taaaagagct agcagaacaa gatttaggaa
      721 aagattcttc tataacagaa ttaaatatcg atgtattcaa tgataaatca aatgaacgag
      781 ttattaatat gatgcgctac cttatttcga gaaataagat gtcatcacct tcgatgcgag
      841 ttttaaatgc tgggattaat atacttttaa atgatgagtc aattaatcca tcgatgtttt
      901 gggatgattg ttgtataaaa agatatcgca ataaacttta ttttctccat gcttctcagt
      961 taaaacccaa tcaaaatctt gaaccagaaa actggaagat aaaaaatcca ttgagattag
     1021 ataatgatgg cggtttgtta gaagttacct tggtaagagg acaaggctta tcgctgagta
     1081 aatgcgatca gaatttaact attaggtatc gcattggagg agagtctatc aaaccggtgg
     1141 gacataaaat aacaaagaag ttgaaaaatt tgttccaaga aaaccatatt ttaccttggg
     1201 tgagggataa gattcctttg atatatagtg gaactgaact cattggtgta gcagatcttt
     1261 ggattcatga tgattacact gcatctcaaa atgagcaagg ttttttgatt gattggcaga
     1321 gaaaaattaa cataaaacat aattgctgat tgttcattgt cacaatatct gttagtttga
     1381 aataccggga tattagtgtt gggaaagcct gagattattt taaaaaaatc ccaagtaaat
     1441 tttaactaat tggaataaaa aacccggaca tgtcacatta tatatttgtt actggaggcg
     1501 tagtatcagc actcggtaag ggtgtaacat ctgcttcact cggtgcaatt cttgaagcca
     1561 ggggattaaa ggttgggatg atgaaacttg atccttatct gaatatagat cccggaacta
     1621 tgagtccata ccagcatggt gaggtttttg taaccgaaga tggtactgaa acggatcttg
     1681 atttaggcca ttacgaacga tatgtaaata ctacaacggg gaaaaacagc aacttcaccg
     1741 ccgggaagat ttacgaaaat gttattgcga aagaaaggaa aggtgattat ctgggtggca
     1801 cggtacaagt gattccccat gtaacagatg agataaagaa aaatatcaca attggtgcag
     1861 atggttttga tgtcacaata attgaagttg gtggaaccgt aggggatatc gaatcactac
     1921 ctttcttgga agccatccgt caaatgggca tagaggaaaa tgggtttaaa gttgcatacg
     1981 ttcatctgac cttggtccct tatataaaaa cttcttcaga aatcaaaacc aagcccacac
     2041 aacattcagt taaagaattg cgatcaattg gtattcaacc cgatgcattg gtgtgtaggt
     2101 cagaaaaacc tctacctgaa gaacaacgga aaaaaatagc tttattcact aatgtaaaag
     2161 aagaagctgt aatttcagct caggatgcaa gtgatattta tgagataccc ttaattttaa
     2221 gggatcaggg attagatgag atcattatca ataaattgca actcaatgtt acaaagccag
     2281 atctgacaga ttgggagagt gttcttcttg ctaagaacag tagtaaatct cttgttaaaa
     2341 ttgcaatggt aggcaagtac gttgatttta gggactcata tatttctttg agcgaagctc
     2401 ttcgtcacgc gggatttaaa acacaaacaa gaattcaaat cgaatacgta gaatctgaag
     2461 agatacaaaa aaatggaacg ggtatcttga aagcaatgga tgcgatttta attccagggg
     2521 gatttggtga tcgtggcatt gagggcatgg tcgactcaat tcagtttgcc agagagaaca
     2581 aggtaccttt tttagggatc tgtctaggtc ttcaagcagc tgtgattgaa ttttcgagga
     2641 ataatctaaa tttattagat gcgaatagca cggaattcga ccccgatact catgcacctg
     2701 ttattgcttt gattaaggaa tggatgaacg aagacggatc cttggagaat agggatgaag
     2761 attctgattt aggagggagt atgaggcttg gtgctcaaga atgtatgatc acaaataaaa
     2821 cattagccag agacgtttac cagaaaagtt cagtcttcga aaggcacaga catcggtatg
     2881 aatttaatat taatttcaaa gaggatctgg agaaaaaagg tttaattttt tcaggtcatt
     2941 cgaaagatgg tttagttgag atcattgagt tggcggatca tccatggttt tttgcttgcc
     3001 aatttcatcc agaatttacg tcatctccaa gaaaaggaca tccactattt gaatctttta
     3061 ttcaagcttc tgtagaatat tcaagaacaa aaaaggaatg atagatgtca gttagtatcg
     3121 gaaaaaataa agtgggtaac aatctacctc taactttgat agcaggacct tgtgtaatag
     3181 aatcattgga gcttgctcaa gaggttgctg gtagcattgc agaaattgcc acaaaattgg
     3241 agataccctt tatatataaa tcctcttttg ataaggccaa ccgaagctct ttgtcaagct
     3301 atagaggtcc aggtattgat gaaggactta aaattttaga aagcgttaaa aagagcgttg
     3361 atgttcctgt tctaacagat gtccatgagg atacaccatt ggaggaaatt gcatcgatag
     3421 tcgatgtttt gcagacaccg gcattcttat gtagacagac aaatttcatt ttaagcgttg
     3481 ctgcacaaaa taagccaatc aatatcaaaa aaggccaatt tctatcaccg tgggaaatgc
     3541 aaaatgttgt taataaagca aaatccgttg gtaataataa tgttttagtt tgtgagcgag
     3601 gttttgcttt tggttataac aatttagtag ccgatatgcg ttctttggtg attttgagag
     3661 aaactaaatg ccctgtcatc tttgatgcaa cacattctgt tcaattgccg ggtggtcaag
     3721 ggtcttcttc gggaggtcag aaagaattta tatcatcatt agccagagct gcagttgcaa
     3781 ctggggtagc aggtattttt attgaaactc accctgatcc agaaaaagca ctgagcgatg
     3841 gaccaaactc tcttccactg aatcaactaa gctctctttt gcatgcctta aaggacatcg
     3901 atagtattgt taagtaaggt cataattttt tagcaaaaga taacgataaa tactcaataa
     3961 aagatctact catgtcagag attaaatcgg tacaaggcag gcaaatacta gattctagag
     4021 gtaatccaac tgtagaggct gagattaccc tacataatgg tatctgtgct agatcatctg
     4081 ttccctcagg agcttcaact ggcagcaggg aagctgtgga acttcgagat ggggatccat
     4141 ctagattttt tgggaaagga gtttctattg cggttaaaaa catcaatact gaaatacaaa
     4201 aagcactgat aggtcaatcg gtattagaac agaaaaagat agataaaact ctgattgaat
     4261 tggattccac agaagataaa tccaggttgg gggcaaatgc aattttggca gtttccctag
     4321 ctgctgctca cacagcggct tctttttcag gaaaaaaatt attccaatac ctgcaatctg
     4381 atgattacag tcttccggtt cccttgatga atatcattaa tggaggagaa catgcagata
     4441 ataatattga tattcaggag ttcatgatcc taccggttgg ggctgaaagc tatagtgagg
     4501 cattgcaata tggtgtggaa gtttttcatt cattaaaatc agtattgaaa aaacaagggc
     4561 taaatacagc cgtaggagat gaaggtggtt tcgcacctaa tcttccaagc aacatcgcag
     4621 cattagatac cattttgcaa gcaatagaat tggcaggttt tcaacctggt ggggatattt
     4681 atttaggttt ggatgttgct agttcggaat tttttgataa tgagaagtac catctttctt
     4741 cagaaggtca aagttttgat tccattggat ttgttgacta tttgtcaaag atttcaaagg
     4801 attatccaat aatcagtatc gaagatggta tgtcagaaaa tgattgggct ggctggcaag
     4861 cattgaccca atccattggt aatgatatcc aactcgtagg tgatgatttg tttgtaacca
     4921 atactagaat atttcaggaa ggaatagaca aaggtatcgc caattctatt ttaattaaac
     4981 ccaatcaaat tggtacatta acagaaacat ttcaagcaat agatatggca aaatcgtcaa
     5041 attatacagc cattatttct catagatcag gtgagacagc ggatacgacc attgcagata
     5101 tcgctgtaag cacatcggtt tcccaaatta aaacaggatc actgtgtaga tcagatcgtg
     5161 tggaaaaata caatcaattg ctaagaattg aagaagcatt ggcacaggaa gcaaagtatc
     5221 taggcaagaa tgcattcggc tctattttta atcgataaca agagcttgga tttatgaaat
     5281 ttataactat catactaatt tttctgttgt tattacttca aatcaatatt tggtttaaag
     5341 acgatggtta tcctcgaatc actgagttaa aatcgctcat tcaggatcag aaaaacgaga
     5401 acagtgcaat ggctacccga aatacccaat tgagggaaga agtggaagat ttgaaaaaag
     5461 gtgatgctgc cattgaagaa agagccagga cagatatcgg tatgattaag gaaggagaag
     5521 aattttattt aatcactcaa cccaagccaa tactggagga aacaaacagt gactagtcat
     5581 gttgaaaggt ctttgtagca aactgtatgg aaattttatt aagcaatgat gatggttact
     5641 attcaaaagg aataagagaa cttgagaaag ttttatccga cagagcaacc attaccatgg
     5701 ttgctcctga gaccaattgc agtggtgcca gtagttccct gacattaaaa aatccattga
     5761 gaataaggaa atttcaaagc aatggttatt atgtcaatgg taccccaagc gattgtgtat
     5821 accttgcatt gacaagtatc gttgaaagta aacctgattt agttattagt ggcatcaatt
     5881 atggacaaaa cttaggagat gatgttttat attctggaac cgttggtgct gcaatgggag
     5941 gtcgttctct tggcgttcct tgtttagcca tttcaattac tagtctaaag cctcaatatc
     6001 ttgaaacggc tctgacagta actaagttat tagtggataa ttatattaat caccgatccc
     6061 tacctaatca agtctacaat attaatattc ccgatattcc ctatgaggag gtacaagaac
     6121 ctctcctgac ccgtttaggg aagagagata ctcaagaagc gataacaagg ccagataaag
     6181 atcgatacgg cgatactata tattgggttg gtggtccacc accaccgaaa gatgcttcag
     6241 agggcacaga tttttttgcc gttcagaaca atcatgtctc aatcacacct ctagtgagtg
     6301 attttaccga ttatcagtcc ttgtcggcaa agcatcgatc aaaggatgga agtgtgcaag
     6361 gatggattgc tcatctttct aatgaattgt tctgatgaag catttgttca caaaattact
     6421 tgatcttgcc ctggacatag catcaaaacc caaagcggtt tattttcttt ttaccatcag
     6481 tttttttgaa tcattttttt ttattccact gccacctgat
//
LOCUS       DASCTH010000212         1670 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000348977, whole genome shotgun sequence.
ACCESSION   DASCTH010000212 DASCTH010000000
VERSION     DASCTH010000212.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000348977"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="QGH35_04530"
     CDS             complement(<1..458)
                     /locus_tag="QGH35_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81833.1"
                     /translation="MPWYLALWVLPLHLYALVFPPLAILVITNHHWSYLEEAIDHPIL
                     LYYAVGFLFLGSLLEVIQNSVDRWYLTADTASALQTSALDGLFTFFIVVGQAMILLAM
                     IGNTGWTLWLVVIVIAATPILYIKQMFPLLPIIITGLLNTIIGYFIFGDP"
     gene            875..>1670
                     /locus_tag="QGH35_04535"
     CDS             875..>1670
                     /locus_tag="QGH35_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013292.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HJL81834.1"
                     /translation="MKKILMMFGLFATFNTSSDTITENPILDENQSYPVIVIHGGAGW
                     SKGKNKQEQDAIKMGLKGALDHGFHVLESGGSSLDAVEIAISTLEDNPLFNAGRGSVY
                     TKDETQEMDASIMSGKDQNAGAVASVSRVKNPIKLARYIMEHTKHVLMAGLGAERIAK
                     QGKLELVDPSYFYSEEKLQRVRKQKAKEIGTVGALAMDKLGNITAGTSTGGMTNKLPG
                     RIGDSPIIGAGTWAQNNVCGVSGTGHGEYFIRFNVAREVCARMEYLN"
BASE COUNT          590 a          284 c          352 g          444 t
ORIGIN      
        1 atcggatccc caaagatgaa atatccaatg atagtattca gtaagcctgt aatgatgatg
       61 ggtagaagag gaaacatttg cttaatataa agaattggag tcgctgcaat aactataaca
      121 actaaccaca gagtccagcc agtattacct atcatggcca gcaatatcat tgcttgtcca
      181 accacgataa aaaatgtaaa tagaccatcc aaggcactgg tttgtaatgc actcgctgtg
      241 tcggcagtaa gataccagcg atctacacta ttttggataa cttctaaaag actccctaaa
      301 aataaaaaac ccacagcata atacaggagt atgggatgat ctatggcttc ctctaagtaa
      361 gaccagtgat ggttggttat aacaagaatg gccagaggag ggaatactaa agcataaaga
      421 tgaaggggca gcacccaaag tgcgagatac caaggcaaat taatatattt ttttagtgag
      481 ctcagagtta tagatccaga aaaacacttt tttaaaatga tacaccataa agatttgtaa
      541 taaaaatcaa ttatcaagca acatgactta aataattttg ttcattgttt tcctggcatg
      601 gttctggatc actattgata aagatccgaa aattggctct atgcgggtac gatttccacg
      661 aaaaatcatc caatgagatc accacgataa gcaaaatgca aaaagtgaaa aaatagttag
      721 cgagcatgtg aattattctc cttatataac ggtgtaatta agatttccag ggcagtaaat
      781 atatgaggta tttttaaaaa aattcaaggg aaaaaattat ttatttttcc tgtaattctg
      841 ttaacttaaa ttaaaggctc tattagattt atttatgaaa aaaatactca tgatgttcgg
      901 gctatttgcg acatttaata catcgtcaga cacaataaca gaaaatccaa tcctggatga
      961 aaaccagtcc tatcctgtta ttgtcattca tgggggagcg ggatggtcca aaggaaaaaa
     1021 taagcaagaa caagatgcta tcaagatggg cttaaaggga gcacttgacc acggttttca
     1081 tgtcctagaa tcaggaggat caagtttgga tgccgttgaa attgcaattt ccaccctcga
     1141 agataatcca ttattcaacg ctgggcgagg ctcggtatat accaaagacg agacacagga
     1201 aatggatgct tcaatcatga gtggcaagga ccaaaatgcg ggtgcagtgg cttctgtttc
     1261 tagggtcaaa aatccaataa aactagcacg atatattatg gaacatacta aacacgtgct
     1321 aatggctgga ctaggtgctg agagaattgc taagcaaggc aaacttgaat tagttgatcc
     1381 aagctatttt tattctgaag aaaaattgca aagagtcaga aaacaaaaag ccaaagaaat
     1441 tggcacagtg ggtgctttgg caatggataa gttgggcaat attacagccg gaacatccac
     1501 cggaggtatg accaataaat taccgggaag aattggtgat tcacccatta ttggagcagg
     1561 cacttgggca caaaacaatg tttgtggagt ttcaggtacg ggtcatgggg aatatttcat
     1621 tcgattcaat gtggctcgtg aagtttgtgc caggatggaa tatttgaatc
//
LOCUS       DASCTH010000213         5404 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000351450, whole genome shotgun sequence.
ACCESSION   DASCTH010000213 DASCTH010000000
VERSION     DASCTH010000213.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000351450"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1901)
                     /gene="nuoG"
                     /locus_tag="QGH35_04540"
     CDS             complement(<1..1901)
                     /gene="nuoG"
                     /locus_tag="QGH35_04540"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HJL81835.1"
                     /translation="MENISKNKISITIDEQEYTVNEGQMLIDVTDQLGIHIPRFCYHE
                     KLSIAANCRMCLIDVENTVNPQPACATPVTNGMVIRTRSDKTQKAQQGTMEFLLINHP
                     LDCPICDQAGECELQDLAYSYGGNQSRFEMIKQTKSNDNLGPLISTDMTRCILCTRCV
                     RFADEVAGLPELGTIGRGDSSNISTYIESTIDHELSGNMIDLCPVGALNNKPYRYTER
                     TWELDQIKSVSPHDCVGSNIQIHIKEGKIKRIVPKCNEDINEIWISDRDRFAFAGIYS
                     NDRLYEPMIHQDGKLKECTWEDATKMFSSELITLRKEDKSNQMAGLISPSCTLDQQYL
                     FSYLFNNLNCHNIDHRIQQVDFRGDSADCALPMMEIKPHEIKNMDNILVIGSDIRKEV
                     PIIAHWLRKAAIKGSTVNFINDQPGDYFFPIDNFVLSSSEALAENIGLVLKASLEENK
                     IDLYADLIHSLQALSKPTSSHYQIAQSLVKGNQNLILSGLTANSHPEYSLIRNYLYVL
                     SQINQSHKGELSHGANAIGAHILGCIPHRNAFGENREGGMNALEISTNNHEVLILYDI
                     EPEDCLYVEELKQAIIGSKKVVYFTPYLNAFMEENADIVFPVNTAYESSGSFINVSGI
                     VQNFTVGAS"
     gene            complement(1904..3190)
                     /gene="nuoF"
                     /locus_tag="QGH35_04545"
     CDS             complement(1904..3190)
                     /gene="nuoF"
                     /locus_tag="QGH35_04545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HJL81836.1"
                     /translation="MTDENAVCFSIIDQPESWKIQTYMSSGGYLTWKQILQGQPGNET
                     RKKIIETIKQSGLRGRGGAGFPTGLKWSFMPEGDDQKYIICNSDESEPGTCHDREILK
                     LNPHALIEGMAISGFAMNATVGYNYVRGEFIKECFPRIEAALIEAYAHGILGENILDS
                     GVNFDLYNFLGAGAYICGEETGLIESLEGKQGKPRFKPPFPANFGLYGQPTTVNNTQT
                     LASIPKIIEKGPKWYASMGSEKSPGTIIFSVSGHVNKPGNFELPLAIPFDVLLDKAGG
                     VIDGKKLKAVIPGGSSTKVLKADVISECTMDYESLQDAGSALGTGAVVVIAEGTCMVN
                     LLKRISQFYYTESCGQCSPCREGTGWMYKILERITQGQGTQNDLSLLLDVAGRIEGTT
                     ICAFGDASAWPVQSFLENFYEEFEYMVKNDGKSLAA"
     gene            complement(3190..3693)
                     /gene="nuoE"
                     /locus_tag="QGH35_04550"
     CDS             complement(3190..3693)
                     /gene="nuoE"
                     /locus_tag="QGH35_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HJL81837.1"
                     /translation="MNEKKLQELPEKVRKEIDEWISKFPEGKQRSAIIGSLHALQKAN
                     NGSLTTELMDTLADYLCLPKMYIYEMATFYSMFQTKAVGKHEISVCTNISCMLSGSDK
                     IVQHIENRLNIKLGESTKDGKFFLKKEEECLAACTGAPMMMVNHQYIENLTIDKVDEV
                     INQLERD"
     gene            complement(3713..4966)
                     /locus_tag="QGH35_04555"
     CDS             complement(3713..4966)
                     /locus_tag="QGH35_04555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012824815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HJL81838.1"
                     /translation="MTDKSNLILNFGPQHPAAHGVLRMVMEMDGEVVVNADPHIGLLH
                     RGTEKLAEIKPYNHSIGYMDRLDYVSMMCCEHPYVLAIEKLLGIEPPARAQYIRVLFD
                     EITRILNHLLWLGCHGLDIGAMALLLYCFREREDLLDCYEAVSGSRMHATYYRPGGVY
                     RDLPDEMPQFQANRFISESKVKKLNYNREGSLLDFIEDFTNRFPARVDEYETLLTDNR
                     MWKQRTVDIGIVSPERAIELGFTGPMLRGSGIEWDIRRKEPYEVYDQLNFQIPVGVNG
                     DCYDRYLVRVEEMRQSNSIIKQCVTWLRDNPGPVIIDDNKLATPKRSLMKHDMEALIY
                     HFKLFSEGYTTPEGETYTSIEHPKGEFGVYIVSDGANKPYRLKIRTPSFVHLSAINEM
                     VKGHMLADVVSVIGTLDIVFGEIDR"
     gene            complement(4963..>5404)
                     /locus_tag="QGH35_04560"
     CDS             complement(4963..>5404)
                     /locus_tag="QGH35_04560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HJL81839.1"
                     /translation="SGTGYSRATLKTKNTHDGSNNDQPKRFAVIYHLLSMSHNWRLRL
                     RIFTGSKNPPSVDSVVSIWPTANWFEREAFDLFGIDFEGHPDLRRILTDYEFIGHPFR
                     KDFPTSGNLEVYYDEDEEKVKYRPVSVTTRPTVPRVIRKEKRNK"
BASE COUNT         1547 a         1133 c          917 g         1807 t
ORIGIN      
        1 tggctagctc ccacagtaaa attctgaaca atgcctgata cattaataaa acttccactc
       61 gattcatagg cagtattaac tggaaacaca atgtctgcat tctcttccat gaatgcgttc
      121 aagtaggggg taaaataaac tacttttttg ctacctatta ttgcttgttt gagttcctcc
      181 acatataggc aatcctcagg ttctatatca tatagtatta atacttcgtg attattagtg
      241 cttatttcca aagcattcat ccccccttcc ctgttttctc caaatgcatt tctatgagga
      301 atacaaccta aaatatgagc cccgattgca ttagctccat gagacagctc acctttatgt
      361 gattggttaa tttgtgacaa aacataaaga taatttctaa tgagactgta ctcaggatgg
      421 ctgtttgcag tgagaccact gaggattaaa ttttggtttc cttttactaa ggattgtgcg
      481 atttgatagt gagatgatgt tggttttgat aatgcttgaa gtgaatgtat taaatcagca
      541 tacaaatcaa ttttattctc ttctaatgaa gcctttaaaa ctagacctat attttcagct
      601 aaagcttctg agctcgatag aacaaaatta tcgataggga aaaagtaatc acctggttga
      661 tcattaatga agtttacagt tgaccctttg atggcagctt ttctaagcca atgggcaatg
      721 atgggaactt cctttcgaat gtctgaacca atgacaagga tgttgtccat atttttaatt
      781 tcgtgaggtt taatctccat cattggtaag gcacagtcgg ctgaatcacc tctaaaatca
      841 acctgttgta tccgatgatc tatattgtgg caattgagat tgttaaaaag gtaagaaaat
      901 agatattgct gatctagtgt acatgatggt gaaatgagcc cagccatttg attactctta
      961 tcttcttttc taagagttat taactcacta gagaacattt tagttgcatc ttcccaagtg
     1021 cactctttca actttccatc ttggtgaatc attggttcat ataatcgatc attactatag
     1081 atgcctgcaa aggcaaagcg gtccctatca gaaatccaaa tctcattaat atcctcatta
     1141 cacttgggca cgattctttt tatttttccc tccttaatgt gtatttgaat atttgaaccg
     1201 acacaatcat gtggtgaaac acttttaatc tgatcaagtt cccaggtcct ttcagtgtat
     1261 cggtaaggtt tgttattgag agctccaaca gggcaaagat caatcatatt ccctgacagt
     1321 tcatgatcta tcgtgctttc tatataagta ctaatattag aagaatcccc cctaccaata
     1381 gttcccaatt ctggaagtcc tgcaacttca tcagcaaatc taacacagcg agtacataag
     1441 atgcatctgg tcatatctgt cgaaatcaga ggtcctaaat tatcatttga ttttgtttgt
     1501 ttgatcattt caaacctaga ttgatttccc ccatagctgt atgccagatc ttgtagttca
     1561 cattctcctg cttgatcaca gataggacaa tctagaggat gattaataag taaaaactcc
     1621 attgtgccct gttgtgcttt ttgggtttta tctgaccttg ttctgatcac cattccattt
     1681 gtaactggag ttgcacatgc aggttgaggg ttcacggtgt tctctacatc aatcaggcac
     1741 attctacagt ttgccgcgat tgagagtttt tcgtgataac aaaaacgagg tatatggatt
     1801 cccagctgat ccgtaacatc gattaacatt tggccttcat tgacagtata ttcctgctca
     1861 tcaatggtta tggatatttt atttttactt atattctcca taatcatgca gccaaagatt
     1921 ttccatcatt ttttaccatg tattcaaatt cttcataaaa attttcgaga aagctctgta
     1981 caggccatgc tgaagcatca ccaaatgcac agattgtagt tccttctatt cttcccgcta
     2041 catctagtag taaggataaa tcgttctgtg ttccttgtcc ttgagtgatc cgttctaata
     2101 ttttatacat ccaaccagtt ccttcacgac atggagaaca ttgtccacag ctttctgtat
     2161 agtagaattg tgaaattctt ttcaggagat tcaccatgca tgttccctca gcaattacca
     2221 ctaccgcgcc cgtaccaagt gcagatccag catcttgaag tgattcataa tccattgtgc
     2281 attccgagat tacatcagct tttaatactt tggttgagga tccgcctggt attacggctt
     2341 tcaacttttt gccatcaata acgccacctg ccttatccaa tagaacatca aaagggatgg
     2401 caagaggcag ttcaaaattt cctggcttgt ttacatgacc tgaaacagaa aagataatag
     2461 ttcctggtga tttttcagat cccatagaag cataccattt aggacctttc tcaataatct
     2521 tgggaatact tgctaaggtt tgtgtgttat tgacagttgt cggttgtcca taaagcccaa
     2581 aattagcagg gaatggtggt ttaaatcttg gttttccttg ttttccttct aatgattcga
     2641 tcagacctgt ttcttcacca catatatagg ctccagcacc caagaaatta tagagatcaa
     2701 aattcactcc tgaatcaaga atattttctc ctaaaattcc gtgtgcatag gcttctatta
     2761 gtgccgcctc tatacgtggg aaacactctt taataaattc accacggaca tagttataac
     2821 ccaccgttgc attcatagca aaaccactga ttgccattcc ttctattaat gcatgaggat
     2881 tgagctttaa tatctcacga tcatgacagg ttccaggttc actttcatca gaattacaaa
     2941 taatgtattt ctgatcatca ccctcaggca taaaactcca ttttaggcct gtgggaaatc
     3001 ctgcaccacc tcggcctctt aaacctgatt gtttaatggt ttctatgatt ttttttctgg
     3061 tttcattacc aggttgaccc tgaagaatct gcttccaggt taaatagcca cctgagctca
     3121 tataggtttg aatcttccaa gattcaggtt gatcaatgat agaaaagcaa acagcattct
     3181 catcagtcat cagtctcgct ccagttgatt aataacctca tctaccttat caatagtaag
     3241 attttcaatg tattgatggt tcaccatcat cattggtgca ccagtgcatg ctgcaagaca
     3301 ttcctcctct ttttttaaga aaaatttacc atcttttgtg gattcaccta gctttatatt
     3361 taatcggttt tctatgtgtt gaacaatctt atccgaacca gataacatac atgaaatgtt
     3421 agtgcagacc gatatctcat gtttacccac cgcttttgtt tgaaacattg aataaaatgt
     3481 tgccatttca taaatataca tttttggcaa acataaatag tcagcaagtg tatccattag
     3541 ttccgtcgtt aagctgccat tatttgcctt ttgaagggcg tgcaaggatc cgatgatagc
     3601 tgatctttgt tttccttccg ggaatttact aatccactca tcgatttctt ttctcacttt
     3661 ttctggcagt tcttgtagtt ttttttcatt catagtcatc aaaaaatttt aatcaacgat
     3721 caatttcccc aaatacaata tcgagagttc ctattactga tacaacatct gcaagcatat
     3781 gccctttcac catttcatta atcgccgaga gatggacaaa actgggagtt cttatcttta
     3841 gtcgatacgg tttattagct ccatctgaaa caatatagac gccgaattcc ccttttggat
     3901 gttctattga agtgtaagtt tctccttcag gtgtcgtata gccttcgcta aacaatttga
     3961 aatggtaaat caatgcttcc atatcatgtt tcattaaact gcgttttggt gttgcaagct
     4021 tattatcatc aataatcaca ggtcctggat tatccctcaa ccatgtaaca cactgtttaa
     4081 tgattgagtt tgactgtctc atttcttcga ctctgacaag ataacggtca taacaatcac
     4141 cattaactcc aaccgggatt tggaaattta actgatcata aacctcgtaa ggttcttttc
     4201 ttctaatgtc ccattcgata ccagaacctc ttaacattgg tccagtaaaa cctaattcaa
     4261 ttgctctttc aggtgaaaca atacctatgt cgactgttct ttgtttccac attctgttat
     4321 cagtgagtaa agtttcatac tcatcaactc tcgctggaaa gcggttggta aagtcttcaa
     4381 taaaatcaag caatgaacct tctcgattat aattgagttt ttttactttt gattctgata
     4441 taaaacgatt tgcctggaat tgaggcatct catccggaag gtctctgtat actcctcctg
     4501 gacgatagta agtagcatgc atacgagagc ccgatactgc ttcataacaa tctaataaat
     4561 cttcccgttc cctaaaacaa taaagcaaca gcgccattgc accaatatcc aaaccatgac
     4621 atccaagcca taatagatga tttagaatgc gggtaatttc atcaaacaaa accctaatgt
     4681 actgagctct tgctggtggc tcaattccaa gtagcttctc aatagcaaga acatagggat
     4741 gttcacaaca catcattgag acatagtcca agcgatccat ataacctatc gagtgattat
     4801 aaggcttaat ttcagccagt ttctccgtac ctctatgaag cagaccaatg tgtgggtcag
     4861 cattgacaac aacctcacca tccatttcca taaccattct caagacacca tgagctgcag
     4921 gatgctgtgg tccaaaattt aaaatgaggt tacttttatc agtcatttgt ttcgtttttc
     4981 ttttctgatt actcttggca ctgttggcct agtggtcaca gatactggtc gatatttaac
     5041 cttttcttca tcttcatcat agtaaacttc tagatttcca gaggttggga aatcttttct
     5101 aaaaggatga ccaatgaatt cataatcagt taatattcgt cttaagtcag ggtggccttc
     5161 aaaatctata ccaaataaat caaatgcttc tctttcaaac caattggcgg ttggccagat
     5221 ggacaccaca gagtcgacgc ttggcgggtt tttactacct gtaaatattc tgaggcgaag
     5281 tctccagttg tgactcattg aaagaagatg atagattaca gcaaaacgtt ttggttgatc
     5341 attgttactg ccgtcatgag tattttttgt tttcaaggtg gctctgctat atccagtgcc
     5401 actg
//
LOCUS       DASCTH010000214         3045 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000352770, whole genome shotgun sequence.
ACCESSION   DASCTH010000214 DASCTH010000000
VERSION     DASCTH010000214.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000352770"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(118..1737)
                     /locus_tag="QGH35_04565"
     CDS             complement(118..1737)
                     /locus_tag="QGH35_04565"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HJL81840.1"
                     /translation="MKITLCQINPTVGAINQNKKSIFDWYHRAVDLGADLVIFPELSL
                     IGYPPQDLLLRNRFIENAKNALDEIAQKSTIPIILGNTMMEDNKLYNCAFFCEKGEII
                     SHYKKRLLPTYDVFDEARYFTSGSEPCVVKVSINGENVYLGLQICEDLWDKNYSCDLV
                     EELKAKGAEIIINLSASPYRVDRLLDRCELIQSKASDNRLSYLYCNLVGAQDELIFDG
                     QSLAYNENGELIAQGKAFEEEILMVDVKNSQTIDLIDSRREEKIYNALVLGVKDYFKK
                     TGHTEAVIGLSGGIDSSLTACIAVDALGIENVHGVSMPSKFSSQHSKDDAKLLSENLG
                     VDYRTISIESIVSSFEESLKTSYNGSELGVAEENIQARARGSIIMALSNKFNWLVLST
                     GNKTELAMGYCTLYGDMNGGLSVISDLSKTDVYALSRWVNEKAGFDCIPINSIEKPPS
                     AELAPNQVDPFDYDVVSPLVTALIDNQKSPSELIKEGADPGLVKDISNRIRINEYKRR
                     QAAPGLRVTSKAFGIGRRVPIVNQFDELNND"
     gene            complement(1751..3040)
                     /gene="asnS"
                     /locus_tag="QGH35_04570"
     CDS             complement(1751..3040)
                     /gene="asnS"
                     /locus_tag="QGH35_04570"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HJL81841.1"
                     /translation="MTTTIKQLTDFEGKEVTLKGWVYNIRSIGKIWFLILRDGTGLLQ
                     CVVVKAETDESIFNLEGELTQESSVTVTGNVRAEPRSVGGFELGVTNIKVHQIADEYP
                     ISHKEHGTDFLMNNRHLWLRSKNQNAVLRVRHQVVKATRDFFDDNGFTLMDSPILTGN
                     SVEGTSTLFELDYFDRSAYLTQSGQLYGEASAMAFGKIYVFGPTFRAEKSKTRRHLTE
                     FWMVEPEMAYCDLDENMDWAEKYVSYVVQWCLDHCKEELKVLERDTSSLEKVVPPFPR
                     ITYNEAAKILKEKGADFEYGSDFGGSDETIISEQFDRPVMIHRWPAEIKAFYMKRDTE
                     NEDLSLGVDMIAPEGFGEIIGGGQREDDHDTLLKRINEHNLPIEPFQWYLDLRKYGSV
                     PHSGFGLGIERTVGWICGTKHVRETIPYPRTMNRIEP"
BASE COUNT          932 a          647 c          455 g         1011 t
ORIGIN      
        1 tatacgccat ttataaatgc aactttttct tttgcttcta acgtgtccca gtaaatagcc
       61 ggggttcgct tttgactcca caaaatgccg aacaaaagac tcacgattat tattttttta
      121 atcattattc aattcatcaa attggtttac gattggcacc cttctaccga taccaaaggc
      181 ttttgaagtt acacgtaatc ccggtgctgc ttggcgcctt ttatattcgt taattcggat
      241 tcgatttgag atatctttta ccaatccagg atcagcaccc tctttaatca attctgatgg
      301 agatttttga ttatctatta gcgcagtgac caaaggacta accacatcat aatcaaatgg
      361 atctacctga ttgggagcca attcagctga gggaggtttt tcaatggaat ttatgggtat
      421 acaatcaaat ccggcttttt catttaccca tcgagaaagg gcataaacat ctgttttgga
      481 taaatcagaa ataacagaaa gacccccatt catatcacca tacaatgtgc agtatcccat
      541 ggccaattct gttttattgc ccgtagacaa tactagccaa ttaaatttat tggatagagc
      601 cataataata cttccgcgag ctcgagcttg aatattttct tctgcaactc ctagttcact
      661 tccattataa ctagttttta aagattcttc aaaagaacta acaatggatt caatggatat
      721 agttctataa tcaacaccta agttttcaga taataatttt gcatcatctt tactatgttg
      781 actagaaaat tttgaaggca ttgaaactcc atgcacattt tctattccaa gcgcatcaac
      841 agcaatacaa gctgttaaac ttgaatctat cccaccactt agtccaatta ctgcttctgt
      901 atgccctgtt ttcttgaaat aatctttaac tccaagaaca agggcattat atattttttc
      961 ttctcttcta ctatctatta aatcgattgt ttggctgttt tttacatcaa ccattaaaat
     1021 ttcttcttca aaagctttgc cttgagcaat caattcacca ttttcattat aagctaaaga
     1081 ctgtccatca aatatgagtt catcttgtgc accaactaaa ttgcagtata aatacgataa
     1141 tctattatca gatgctttgc tttgaataag ctcacaacga tctaacaatc tatcaactct
     1201 ataaggtgaa gcagataaat tgataattat ttcagcgccc tttgctttaa gctcttcaac
     1261 tagatcacat gaataatttt tatcccaaag gtcttcgcaa atttgcaaac ctaaataaac
     1321 attttcgcca ttgattgata ctttaacaac gcaaggttca ctgccacttg taaaataacg
     1381 agcttcatca aatacatcat acgttggcaa taatcttttt ttataatgac tgataatttc
     1441 acctttttca caaaaaaatg cacaattata taatttatta tcttccatca tagtattccc
     1501 taaaataata ggaatagttg atttttgggc aatttcatct aatgcattct ttgcattttc
     1561 aataaaacga tttcgaagta ataaatcttg aggaggataa ccaattagag ataattctgg
     1621 aaaaataaca aggtcagcgc ctagatcaac agcacgatga taccaatcaa aaatactctt
     1681 tttattttga ttgattgctc caacagttgg gtttatctgg caaagggtaa ttttcataaa
     1741 taatatctgt ttatggctct atcctattca ttgttcgtgg gtaaggtatt gtttctcgaa
     1801 catgtttggt tccacatatc catccaactg ttctctcaat gcctaaacca aaccctgaat
     1861 gtggaacaga accatatttt ctcaaatcaa ggtaccattg aaaaggttca atgggaaggt
     1921 tgtgttcatt aatacgtttt agaagtgtgt catgatcatc ttctctttga ccgccaccaa
     1981 tgatctcacc aaatccctct ggcgcaatca tatcgactcc taaagataaa tcttcattct
     2041 cagtatctct tttcatatag aaagctttaa tctcagctgg ccagcgatga atcattactg
     2101 gtcggtcaaa ctgttctgag ataatggtct catcagaacc tccaaaatca gaaccgtatt
     2161 caaaatcagc gcctttttcc ttaagtatct tcgcagcctc attgtatgta attctaggaa
     2221 acggtggtac aaccttttct aatgatgatg tatctcgctc aagcactttt aattcttctt
     2281 tacagtgatc caaacaccat tgaacaacat agctcacata tttctctgcc caatccatat
     2341 tttcgtccaa atcacaataa gccatttccg gctcaaccat ccagaattca gttaaatgac
     2401 gacgtgtttt ggatttttct gcacgaaacg tggggccgaa aacatatatt ttcccaaatg
     2461 ccatggcgct ggcttctcca taaagctgtc cactttgtgt taaataagct gatcgatcaa
     2521 aataatccaa ttcaaataga gtactggttc cttccacaga atttccggtt aaaatgggag
     2581 aatccatgag tgtgaaccca ttatcatcaa aaaagtcccg agtagctttc actacttggt
     2641 gacgaacccg taaaaccgca ttctgatttt tagatcgaag ccatagatgt cgattattca
     2701 tcaaaaaatc tgtcccatgc tccttatgag aaatgggata ttcatctgca atctgatgga
     2761 cttttatatt cgtaaccccc aattcaaatc cgccgactga gcgaggttca gcccggacat
     2821 tccctgtaac agtgaccgat gattcttgtg ttaactcacc ctctaagttg aatatacttt
     2881 catctgtttc tgcctttaca acgacgcact gtagaagacc agtgccgtct cgtaaaatta
     2941 aaaaccatat ttttccaata gatcgaatat tatacaccca tcctttaagg gtgacttcct
     3001 ttccttcgaa atctgttaat tgctttattg ttgttgtcat tttgc
//
LOCUS       DASCTH010000215         1925 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000355594, whole genome shotgun sequence.
ACCESSION   DASCTH010000215 DASCTH010000000
VERSION     DASCTH010000215.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000355594"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(115..1296)
                     /locus_tag="QGH35_04575"
     CDS             complement(115..1296)
                     /locus_tag="QGH35_04575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HJL81842.1"
                     /translation="MIKKFFKWLGIGIFIIIVGGFLPATLGAYGLFWERWTTSLLGNP
                     LNPKLSWYTPQETISGNYSEALLTSKDSEIPPNVFQEANDYAELHGSDGLVIQHQGEI
                     VFENYWNDKQPDSLFALHSMTKTMNALLMGHAIADGFIDSVDIPAATFLVEWKGSEKE
                     AIQIRDLLNMASGLQESYDFSPSSARIQRMMGLDIIQPNIDVGIDGMPGQVFSHVNPN
                     SQLLGIIIERASGQRISDYLSEKIWKKIGARDALFYVDKPGGMVHTDCCMWASIRDMV
                     RIGEMLMHKGVFQGRQIMPSGWIEEMIQPSKANVNYGMQLWMGNDFAEYRPYDARMTT
                     FANYHSEPYKADDVFYLDGLGKKRLYIIPSKSLVILRTGTNSKEWDDAKLPNLFIEAL
                     K"
     gene            complement(1309..>1925)
                     /locus_tag="QGH35_04580"
     CDS             complement(1309..>1925)
                     /locus_tag="QGH35_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012371.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HJL81843.1"
                     /translation="FMNGDDTQNIALNQPLEQTPNSEFAYNGINPQNLGLLLERATKK
                     SYSDYLSENLWRYMKDDAFVTLDAEKHATTRMFCCLDATARDLLRIGLLILNQGRLGD
                     SRIVSETWIREMITPSDLNPNYGYLTWLGTEYQENRIYNNKSSATGFHSEPFVDEDIV
                     FLDGFGGQRAYIIPSKDLVIIRTGAIVWDWDDAKLPNIISKGVN"
BASE COUNT          534 a          413 c          336 g          642 t
ORIGIN      
        1 gattctttag tcgtcgtcac actaacagta tgaccaaggt tttttagata ttttattgct
       61 gccaaccctg tatatgcagc accaactatg gctatattca taaacttctc tcctctattt
      121 gagtgcttcg ataaatagat tgggcaattt agcatcgtcc cattcttttg aatttgttcc
      181 tgttcttagt attaccaaag atttagatgg aatgatatac aatctttttt tacccaggcc
      241 atccagataa aaaacatcgt ctgccttata tggttccgaa tgataatttg caaacgttgt
      301 cattcttgca tcataaggcc gatactcagc aaaatcgttt cccatccata attgcatgcc
      361 gtaatttaca ttagctttag atggttggat catttcttct atccagccag aaggcataat
      421 ttgcctgccc tggaatactc ctttatgcat cagcatctct ccaatgcgaa ccatgtctct
      481 aatcgatgcc cacatgcaac aatcagtatg taccataccc ccaggtttat cgacataaaa
      541 caaggcatct cttgctccaa tttttttcca aattttttct gagagataat cactgattct
      601 ctgacccgat gctctttcta tgatgatgcc aagcagttga ctgttgggat tgacatgtga
      661 gaatacttgt ccaggcatac catcaatgcc aacgtcaata ttaggttgaa tgatgtccag
      721 acccatcatt ctctggattc ttgctgagct gggtgagaaa tcatagcttt cttgcaatcc
      781 gctggccatg ttgagcaaat ccctgatttg aatagcttcc ttttctgagc ctttccattc
      841 cactaggaat gttgctgcag gaatatcaac tgaatcaatg aagccatctg ctatggcgtg
      901 acccatgagc aaggcattca ttgttttggt cattgaatgg agtgcaaata aactatcagg
      961 ttgtttgtca ttccaatagt tctcaaagac gatttctcct tgatgttgga taaccaaacc
     1021 atcactaccg tgaagctctg cataatcatt ggcctcttga aatacattcg gtggtatttc
     1081 actatctttc gatgttaata gtgcctcgga ataatttcct gagattgttt cttgaggggt
     1141 gtaccaagat aattttgggt tgagtggatt acccagtaat gatgttgtcc acctctccca
     1201 gaacaaacca taggcaccca atgtagcagg taaaaaacct ccaacaatga tgataaagat
     1261 tccgataccc aaccatttga aaaatttctt gatcatgatt tttcctactt agtttactcc
     1321 tttgctgatt atatttggta gtttcgcatc atcccagtcc catactattg ctcctgttct
     1381 aataattaca agatctttag aaggaatgat ataggctcgt tggccaccaa agccatctaa
     1441 aaatacaata tcttcatcta caaaaggctc agaatgaaat ccagtcgctg agcttttatt
     1501 gttatagatt ctgttttctt ggtattcagt gccaagccaa gttaaataac cataattagg
     1561 gtttaaatct gatggagtta tcatttcacg aatccatgtt tcagatacaa ttcttgaatc
     1621 acctagtcta ccttgattta gaatcaacag tccaattcta agcaagtccc tggctgttgc
     1681 atccaagcag caaaacattc ttgttgttgc atgtttctct gcatccaatg tcacaaaggc
     1741 atcgtccttc atataacgcc ataaattttc agacagataa tccgagtaac ttttcttagt
     1801 ggctctctct agtaacagtc cgagattttg tgggttaata ccattgtagg caaattcaga
     1861 atttggtgtt tgctctagag gttgatttaa tgcgatgttt tgggtgtcat ctccattcat
     1921 aaatt
//
LOCUS       DASCTH010000216         4978 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000356749, whole genome shotgun sequence.
ACCESSION   DASCTH010000216 DASCTH010000000
VERSION     DASCTH010000216.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000356749"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="QGH35_04585"
     CDS             <1..347
                     /locus_tag="QGH35_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003631718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HJL81844.1"
                     /translation="RDFAEVRAQGDINQKVANEAMELMDVDQHGLDVLDRKFLIAIIE
                     RFNGGPVGIGAVAAAIGEDRATLEDVIEPFLIQRGFLLRTPRGRSASIHAYKHFGIEV
                     PKEIKEQNRSLF"
     gene            complement(382..456)
                     /locus_tag="QGH35_04590"
     tRNA            complement(382..456)
                     /locus_tag="QGH35_04590"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Glu,seq:ctc)
     gene            506..1174
                     /locus_tag="QGH35_04595"
     CDS             506..1174
                     /locus_tag="QGH35_04595"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008291796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HJL81845.1"
                     /translation="MGNVVPLKLESRLVVIEGPIGVGKTSLAKRLSDHVSGKLILEEP
                     ENNPFLENFYLDQESNALPTELSFLFQRAKHIEGINQDDLFSDTTIADFFFDKGSIFS
                     ELNLNAEELHLYQQVKDSLRVNPPTPDLVIYLQAPVDVLISRLHSRQTSVDRLIESSY
                     LEKLADSYAKFFYDYDDSPLLVVNAESIDPVHNDDHFLLLYEEVVSVKYGKHFFNSTA
                     AAFT"
     gene            complement(1196..2257)
                     /gene="queG"
                     /locus_tag="QGH35_04600"
     CDS             complement(1196..2257)
                     /gene="queG"
                     /locus_tag="QGH35_04600"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HJL81846.1"
                     /translation="MAQNPNAIKTLIHQWSKELGFDGFGVTGIEIHKDEKHLQSWLNK
                     KFHGSMSYMEKHGLKRSRPDVLLPGTIRVISLKMHYFSRDQDQAFELLKKNEVAYIAN
                     YAVSTDYHQIIKKRLKVIIGKIRDYSELQGQNYFVDSAPVLEKALARNAGLGWIGKNT
                     NLIHSSSGSWFFLAEIFTDIPLPIDRSSKNHCGTCSACIDICPTQAFTMPYQLDARKC
                     ISYLTIENKQSIPVELRPVIGNRIFGCDDCQIICPFNKFAKKIEMNDLLPKGTLLDQP
                     LEQLFLWSEEEWENHTQGTALTRPGYDGWLRNIAVALGNARTTERIVDALRLRNNRQS
                     PMVNEHIFWALEQHQKKSN"
     gene            2307..3794
                     /locus_tag="QGH35_04605"
     CDS             2307..3794
                     /locus_tag="QGH35_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013425.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HJL81847.1"
                     /translation="MKISDNYVYPSIEVSKIDNLAIEQEGIPGFVLMRRAADFAYKSA
                     IECYPNTNSFIVFCGLGNNAGDGYLFASRALSEGKKAQVVYLSAPENLKGDAYLAYQE
                     YEKLGGQIFPWTKDRELCADLFVDAIFGVGLNRSIDGVFLQAIQTINQSTIPVISLDI
                     PSGLNPDTGEKMGDCVSAQITTTFVGYKKGLFLNSGPDCSGKITYSDLDIPKSCFDSA
                     KPSLRIVDKELSKTLLKKRPITSHKGSFGHILIVGGNHGMGGAVRIASEAALRSGAGL
                     VSVVTRAANIPIVTKLRPEIMCHSVDEDPNKINDFIAKASVVAIGPGLGLDDWAMTLF
                     EQVFQSNLPMVVDADALNILSLKPRKRKNWILTPHPGEAARLIGLTNPRVQSDRIGAL
                     KKLSDRFSGTIVLKGNNTLIGSSNDMPYLITVGNPGMASAGMGDLLTGIIAGLLGQFK
                     DIDQGLVSAVAAYIHGFSGDLAAEQGERGLIATDLFKHLQQCLNP"
     gene            3779..4264
                     /gene="tsaE"
                     /locus_tag="QGH35_04610"
     CDS             3779..4264
                     /gene="tsaE"
                     /locus_tag="QGH35_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HJL81848.1"
                     /translation="MLESVKTLFDDTETMNCGSQLARCFSDSSNDSHVIYLKGDLGAG
                     KTTFAKGFLKGMGYEGNVTSPTYTLIEVYRTEFYDIYHVDLYRIKNRKEIFELELNEE
                     PISSSPYIFLIEWPENGLPDILAANFEISFSVEESLDQRTMNVICNTKKSSNILTSWL
                     K"
     gene            4391..>4978
                     /locus_tag="QGH35_04615"
     CDS             4391..>4978
                     /locus_tag="QGH35_04615"
                     /inference="COORDINATES: protein motif:HMM:NF023169.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HJL81849.1"
                     /translation="MRINAEKDKTRLVLDLSNEVRYKILTLNNPNRLVVDLYGARKTN
                     KIKSSTKGNGLIDTIRVAKNTPAKLRVVIETKQVVLYKVNMLKASQKRDSRLVIDLKG
                     MYEGSQKVAASAINNSKMIIAVDPGHGGKDPGATGKKIKEKDLVLKISKRLAHLINAE
                     PDMTAILTRNDDSFPCPNNNRSCGQKTSLNERLEIA"
BASE COUNT         1557 a          850 c          998 g         1573 t
ORIGIN      
        1 caagagattt tgcggaggtt agggcacagg gagatattaa tcaaaaggtt gccaatgagg
       61 caatggaatt gatggatgtt gatcaacatg gtttggatgt attggatagg aaatttttaa
      121 tagcaattat tgaaagattt aatggtggtc ctgtgggcat tggagctgtt gctgcagcga
      181 tcggcgagga tcgagccacc ttagaagacg taattgagcc ttttttaata caaagaggat
      241 ttttattaag aacaccaaga ggcaggtcgg cttcaattca tgcgtataaa cattttggta
      301 ttgaggtacc taaggaaatt aaagaacaga atagatccct tttttaggaa tcaaagtaag
      361 gagttttaaa ttggtttcca atggcgctcc ctaggggatt tgaacccctg tttccgcctt
      421 gagagggcag cgtcctgacc actagacgaa gggagctagt aggtgttata aaagtatatg
      481 taattatttt gttagtataa agattatggg taatgttgta ccactcaaat tagaatctcg
      541 acttgttgta atcgaaggtc cgattggtgt tggtaaaaca agtctggcga agcgtttgtc
      601 tgatcatgtt tccggaaaat taattttgga agaaccagaa aataatcctt ttttagagaa
      661 tttttatctt gatcaagaat caaatgcatt acctactgag ctgtcttttt tatttcagag
      721 agccaaacac atagagggta tcaatcagga tgatttattt tcagatacta ccattgctga
      781 tttctttttt gataagggtt ccatattttc agaactaaac ctgaatgctg aggaactgca
      841 tttatatcaa caagtaaaag actctcttcg agtcaatcca cccactcccg atctagtgat
      901 ctatttacag gcacctgtag atgtattaat ttctagactc catagcaggc aaacatcagt
      961 tgaccgttta attgaatcaa gctatctgga gaagctcgca gattcttatg caaaattttt
     1021 ctatgattat gatgattcac ccttattggt tgtcaatgca gagagtattg atcctgtaca
     1081 taatgatgat cattttcttt tgttgtatga agaagtggtt agtgtgaaat acgggaaaca
     1141 cttttttaac tctaccgcag ctgccttcac ataatctctt tgtccgatca gtatattaat
     1201 tagatttttt ctgatgttgt tccagtgccc aaaagatatg ttcattaacc attggagatt
     1261 gacgattgtt tcttaatctc agtgcatcca caattctttc tgttgtccta gcattgccca
     1321 atgccactgc aatatttctg agccaaccat cataaccagg tctagttaat gctgtgcctt
     1381 gggtatgatt ttcccattct tcttcagacc acagaaagag ttgttctaag ggttgatcta
     1441 atagtgttcc tttaggtaac agatcattca tttcaatttt cttggcaaat ttattaaagg
     1501 ggcagatgat ctgacaatca tcacagccaa agattctgtt accaattact ggtcttaatt
     1561 caacaggtat ggattgttta ttctctatcg ttaagtaaga aatacacttt cttgcatcga
     1621 gctggtaagg catggtgaaa gcttgagttg gacaaatatc aatacaagcc gaacaagtac
     1681 cacagtgatt ttttgaagat cgatcaatcg gaagtggaat atcagtaaaa atctctgcta
     1741 gaaaaaacca agatccactg gaactatgga ttaaattggt attcttgcca atccaaccta
     1801 gtcccgcatt tctagccaaa gctttttcca agacaggggc agagtcaaca aaataattct
     1861 gcccctgtaa ttctgagtaa tctctaattt taccaattat tactttgagt ctttttttaa
     1921 ttatttgatg atagtcagta cttactgcgt aatttgcaat atatgcaacc tcattttttt
     1981 ttagtagttc aaaagcctga tcctggtccc ttgagaagta atgcattttt aatgaaatta
     2041 ccctaatcgt tccaggtaac aaaacatcag gtcgagatcg tttgagtcca tgtttttcca
     2101 tatagctcat cgatccatgg aatttcttgt tcaaccacga ttgcaagtgt ttttcatctt
     2161 tgtgtatctc tataccagta acaccaaagc catcaaatcc aagctcttta gaccattggt
     2221 gaataagagt ttttatagca ttgggatttt gggccatttt ctttctctga gagagtttta
     2281 tttttaagca aagtataatg attagtatga agatttcaga taattatgta tatccatcta
     2341 tcgaagtctc aaagattgat aatctagcta tcgaacaaga aggtattcct ggatttgttt
     2401 taatgagacg tgctgctgat tttgcttaca aatcagctat agaatgctat ccaaacacaa
     2461 actcgttcat tgttttttgt ggtcttggaa ataatgcagg ggatggatat ttatttgcgt
     2521 cgcgggcctt aagtgaaggg aagaaagccc aggtagttta tttatcggct cccgaaaatc
     2581 ttaaaggaga tgcttatctt gcatatcagg aatatgagaa attaggaggc cagattttcc
     2641 cgtggactaa agatagagag ttatgtgcag atctattcgt tgatgcaatt tttggtgttg
     2701 gtttgaatcg atcaatcgat ggcgtttttc tgcaagcaat acaaactatt aatcaatcaa
     2761 caatcccagt tatttcattg gatattcctt ccggtctaaa tcctgatact ggcgagaaaa
     2821 tgggagattg tgtttctgca caaataacaa caacctttgt tggttataaa aaaggtctct
     2881 ttttaaattc tggaccggac tgctctggga aaattactta ttctgattta gatatcccta
     2941 agagttgctt tgattctgca aagccctcgt tgagaattgt agataaagag ttatcaaaaa
     3001 ctcttttgaa aaaaagacct ataacatctc ataaaggcag ctttggccac atcttgattg
     3061 ttggcggtaa tcatggcatg ggtggagcag ttagaattgc atcagaggcg gctcttcgct
     3121 ctggagcagg attggtaagt gtggttacaa gagctgccaa tattcccatt gtgactaaat
     3181 taaggccgga aataatgtgc cactctgtag atgaagatcc taataaaata aatgatttta
     3241 tcgctaaagc ctctgttgtt gcaattggcc ctggattggg attggatgat tgggcaatga
     3301 cattattcga acaagtattt cagtcaaatt tgcctatggt tgtggatgct gatgcactca
     3361 atattttgtc tcttaaacct agaaagagga aaaactggat tttaacacct catccaggtg
     3421 aagcagcaag attaataggt ttaactaatc ctagagtgca atctgataga ataggtgccc
     3481 ttaagaaact ttcagatcgt ttttctggaa caattgtact gaaaggaaac aatacattga
     3541 taggatcctc aaacgacatg ccttatttaa ttacagtggg caatccgggg atggcttcag
     3601 caggaatggg tgatcttctg acagggataa ttgcaggtct acttgggcaa tttaaagata
     3661 ttgaccaagg attggtatcg gcggtggctg cctatatcca tggattttct ggtgaccttg
     3721 ctgcggaaca aggggaaaga ggactcatag ctaccgattt gtttaaacat ttacaacaat
     3781 gcttgaatcc gtaaaaacct tatttgacga tacagaaaca atgaattgtg gctctcaact
     3841 ggccagatgc ttttctgact catctaatga ctctcatgta atttatctca aaggtgatct
     3901 gggtgcagga aaaactactt ttgctaaggg atttctgaaa ggcatgggct atgaaggcaa
     3961 tgtgacaagt cccacatata ctttgattga agtttatcga acagaatttt atgacatata
     4021 tcatgtcgat ctttatagaa tcaaaaacag aaaagagatt tttgaactgg aacttaatga
     4081 ggagccaatt tcatcaagcc catatatttt tttaattgaa tggcctgaaa atggtttacc
     4141 ggatatctta gctgccaatt ttgagattag tttttctgta gaggagtcac tggatcaaag
     4201 aactatgaat gtgatttgca acacaaaaaa atcaagcaat attctaactt cttggctaaa
     4261 gtaattggca ttgaaaataa cacatggtac agacataaat aaaaatacga aaacttactc
     4321 actttttctc cttttaattt ttgtgctttt ttccagcaag atgaccgctg ctcaaacaac
     4381 attaaaagat atgaggatta atgctgagaa agacaaaact cgtttggttc ttgatttatc
     4441 aaatgaggtt cgatacaaaa ttcttacatt gaacaacccc aaccgcttag tagtcgatct
     4501 atatggggca agaaagacca ataagatcaa atcctcaact aaaggaaacg gtttaatcga
     4561 cacgataaga gttgcaaaaa atacaccagc taaattgaga gttgttatcg aaaccaagca
     4621 ggtcgttctc tacaaagtca acatgcttaa agcaagtcaa aaaagagact ctagattggt
     4681 gattgattta aagggtatgt atgaaggaag tcaaaaagtt gctgccagtg ctattaataa
     4741 ttctaagatg attattgctg tagaccctgg gcatggaggt aaagaccctg gtgcaacagg
     4801 caaaaaaatt aaagaaaagg atttagtttt aaaaatctca aaacgtttag cacacttaat
     4861 caatgctgaa ccagacatga ctgctatttt gactagaaac gatgattctt ttccatgccc
     4921 gaataacaat agatcttgtg gacagaaaac ctcattaaat gagcgtttag agatagca
//
LOCUS       DASCTH010000217         1548 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000363261, whole genome shotgun sequence.
ACCESSION   DASCTH010000217 DASCTH010000000
VERSION     DASCTH010000217.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000363261"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="QGH35_04620"
     CDS             <1..472
                     /locus_tag="QGH35_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81850.1"
                     /translation="IPEPVPESLLNDLIAFIMVETDAPNKPNIETPVLDQLVDRELPL
                     SERFPSFWNHLMNNTTHYRLVTANHELEFDAYFNTDGSVGTSLESVHGFWHVNERDMF
                     CYALYGLPVVVDQFVECFPVAAMAIPRFSRELWYSEPQTGVQLYGGIMPGRPER"
     gene            492..1448
                     /locus_tag="QGH35_04625"
     CDS             492..1448
                     /locus_tag="QGH35_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014635.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HJL81851.1"
                     /translation="MVDYVKLSEVLTGKVRGNPVAISLFEGTIPKVYQQQKVVPCAIV
                     RHAMDKGEIVSFDKYHHDCITGVYTAGVDPGTEEIRNGQYLAQNIPTYTDIAAEKVKT
                     GDYVLPQNTVIGIGAAPLADVPENVTVDWIVVVCTPYWANYIGGARTVLDGTPARGAC
                     GSSFCSDLFATPWHDDNVVITPGDVGGRMNNRIKPEELFVVVPSKYLDSLFNILTSTP
                     DARKLLEATKPEDSDYWEKRKRAEQKKQARSSSLEGLDAKLSMQWDQEAKDMIAATPP
                     GIVEMAINNVEDFARDNGVERITKFVVWDQMKSVGMDPNILN"
BASE COUNT          450 a          274 c          379 g          445 t
ORIGIN      
        1 gattccagaa ccggttccag aaagcctttt gaacgatctg attgcattca ttatggttga
       61 aacagatgct ccaaataaac ccaatattga gacaccagta ttggatcaat tggttgatcg
      121 agaattgcca ttgtcagagc gatttccaag tttttggaac catttgatga acaataccac
      181 ccattaccgt ttggttacag ccaatcatga gttggagttt gatgcttatt tcaacaccga
      241 cggttccgtt ggaacgagtc ttgaaagcgt ccatgggttt tggcatgtga atgaaaggga
      301 tatgttttgt tatgcattgt atgggcttcc agttgttgtg gatcaatttg tcgagtgttt
      361 cccagttgca gctatggcaa taccccgttt ttctagagag ctttggtatt ctgagcctca
      421 aacaggagtg caattgtatg gcggcataat gccaggaaga ccagagcggt aaaaaaaagt
      481 tataattagc tatggttgac tatgtaaaac tgagtgaagt gctgactggt aaggtcaggg
      541 gtaatcctgt tgctatcagt ttatttgaag gaacaatccc gaaggtctat caacaacaaa
      601 aagtagttcc ttgtgcaatt gttcgtcatg caatggataa aggtgagatc gtttcttttg
      661 acaaatatca tcatgattgc atcaccggtg tttatactgc cggtgtggat ccaggcactg
      721 aagagatcag gaatgggcag tatcttgcac aaaatattcc cacttatacc gatatcgctg
      781 ctgagaaagt taaaacgggg gattatgttt taccgcaaaa cactgttata ggaatcgggg
      841 ctgctccact agctgatgtc cctgagaatg tcacagtaga ctggatcgtt gtagtgtgta
      901 ctccatattg ggccaattat ataggagggg ctcgaacggt tctggatggc actcctgcca
      961 gaggtgcctg tggctcatcc ttttgtagcg atctctttgc aacaccatgg cacgatgata
     1021 atgttgttat tactcctggc gatgtagggg gtcgcatgaa taaccgaatt aaacctgagg
     1081 agttgttcgt ggttgtgccc agtaagtatc tcgatagttt atttaatatt ttgacatcta
     1141 ctcctgatgc tagaaagttg ttggaagcaa ctaaaccaga agattccgat tactgggaaa
     1201 agagaaaaag ggctgagcaa aagaaacaag ctagatcttc atctttagaa ggattggatg
     1261 caaagttaag tatgcagtgg gatcaagaag caaaagatat gattgccgca actccacccg
     1321 gcatcgtcga aatggcgatc aataacgttg aagattttgc cagagataac ggggttgaac
     1381 gtattactaa atttgttgtc tgggatcaga tgaaaagtgt tggtatggat ccaaacatcc
     1441 tcaattagat tttatgatta gcaagacttg tttaataaca ggtacagcaa gtggcatggg
     1501 aaagattgct gcagaagcac tggctgaaaa aggggctaaa ttaattct
//
LOCUS       DASCTH010000218         2686 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000365744, whole genome shotgun sequence.
ACCESSION   DASCTH010000218 DASCTH010000000
VERSION     DASCTH010000218.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000365744"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="QGH35_04630"
     CDS             <1..285
                     /locus_tag="QGH35_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024761.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HJL81852.1"
                     /translation="SQNFSLRKGCDPFTVGLDEALTSIASKKGSTNIKEFEDSNIKIK
                     EGRWGPYITNGKLNVSVPKNTDPKTLTEEDCLALLDAKQKKNKNKKKKKR"
     gene            311..862
                     /locus_tag="QGH35_04635"
     CDS             311..862
                     /locus_tag="QGH35_04635"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HJL81853.1"
                     /translation="MFSSSDYKNIHDVLIHGGLIAFPTESIFGLGCDPGNKKAVEKLK
                     RIKNRPQEMGFILLTPNLATISQWIDPDKKQTEIISAPVQRPTTYIVPASKSVPPWLT
                     TNNTLAIRINKDPIIQGLSDMLGLPIISTSANQHGKTPCQSASEVQKTMGPLLDYIIE
                     GEKGSFGRPSTIIDLCSGKTIRP"
     gene            920..1735
                     /gene="aroE"
                     /locus_tag="QGH35_04640"
     CDS             920..1735
                     /gene="aroE"
                     /locus_tag="QGH35_04640"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HJL81854.1"
                     /translation="MDNYGVIGNPIKHSKSPEIHQLFAKQVGHDILYHRLPATGESFT
                     SVLEEFFKKGGKGLNITLPFKEKAFRFADSCSDNAQSCGSVNTLTFSEDGRTHGDNTD
                     GIGLINDLKNNDVLLENIKVLILGAGGATRGIVPVLFNNNVGSIALHNRTMEKAELLQ
                     EEFMPIGTIEPLSSDGLAGGFGLVINATSASLDGVVPDIPDSVVSNAVCYDLAYSYGE
                     TPFLNWAKKNGSAKNLQGLGMLVEQAAESFFIWRGVRPRTSEVVDKLRSELLT"
     gene            complement(1732..2010)
                     /locus_tag="QGH35_04645"
     CDS             complement(1732..2010)
                     /locus_tag="QGH35_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81855.1"
                     /translation="MKKSILICLLSLPGALLFAGDHDLLDENACNETKEGIGGALGIA
                     DYLFKEVERLDKEKPKNEQKQKELFEAALAFSEIAENYSTVYAVWCKD"
     gene            2069..>2686
                     /gene="xth"
                     /locus_tag="QGH35_04650"
     CDS             2069..>2686
                     /gene="xth"
                     /locus_tag="QGH35_04650"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HJL81856.1"
                     /translation="MKIASWNVNGIRARVEHVKHWLTISRPDILALQETKVVDDLFPF
                     DAFESIGYQAVVFGQKAYNGVALLTKNKAPNITRGMDGYQDEQARVISADYKGIRIID
                     VYVPNGQSVGSEKFAYKIEWFSHLHRLIKQSFQANKNLVVLGDFNIAPEDIDVYDPEV
                     WKDKVLCSDQERTCLRSLLSLGLSDSFRLFEQLEEQYSWWDYRAAA"
BASE COUNT          845 a          538 c          552 g          751 t
ORIGIN      
        1 tcccaaaatt tctcacttcg aaaaggctgt gatcctttta cagttggatt ggatgaggcc
       61 ttgactagca ttgctagcaa aaaaggcagc acaaacatca aggagtttga agattcaaat
      121 attaaaatta aagaggggcg ctgggggcct tatatcacca atggaaaatt gaatgtttcg
      181 gtgcccaaaa atacagaccc aaaaaccctc actgaagaag actgtcttgc tcttttggac
      241 gcaaaacaga aaaaaaacaa aaacaagaag aaaaagaaga ggtaattata aaatacacag
      301 gacatgaatc atgttttctt cttctgatta taaaaatata catgacgtat tgatccatgg
      361 agggttaata gctttcccaa cagagtcgat atttggacta ggatgtgatc ctgggaataa
      421 aaaagctgta gagaaattaa aaagaattaa aaacagacct caggaaatgg gctttatcct
      481 gctcacccca aatctggcaa ccatatccca atggattgac cctgataaaa aacaaaccga
      541 aataatttcc gcgcctgtac agcggcccac aacctatatt gtgccggcca gtaaatcagt
      601 acctccttgg cttaccacta acaacacatt agcgatccga ataaacaaag atccaattat
      661 ccaaggtttg tcggatatgt tgggtcttcc aattatctcg accagtgcaa accagcacgg
      721 caaaacacct tgtcagtctg ctagtgaggt tcaaaaaact atgggccctc tgctggacta
      781 tattattgaa ggagagaaag gttcctttgg gcgcccctcc acaattattg acttatgttc
      841 tggaaaaacc atacgtcctt aattgtcagc cacgacaaat aacagctata ctcattaaaa
      901 cctttcatag agcatatata tggataatta tggcgttata gggaatccga tcaagcacag
      961 caaatcccca gaaatccatc aattgtttgc caaacaagtt gggcatgaca ttttatatca
     1021 tcgcctacca gcaacaggtg aaagttttac tagtgtgctt gaggagtttt ttaaaaaagg
     1081 agggaaaggg ttaaacatca cgcttccttt taaagaaaaa gccttcaggt ttgctgattc
     1141 atgctcggat aatgcccaat cctgcggctc cgtaaacacg ctaacctttt ctgaggatgg
     1201 aagaactcat ggagataata cggatggtat tggcctcatt aatgatctaa aaaataatga
     1261 tgtgctgtta gaaaacataa aagttctcat actgggcgcc gggggggcaa ccagaggcat
     1321 agtgcctgta ttattcaata ataatgtagg ctctattgct ctgcacaacc gaaccatgga
     1381 aaaagcggag ttgttacagg aagaatttat gcctattgga acaattgagc ctttatcttc
     1441 tgatggtctt gccggtggtt ttggtttggt gattaatgcc acatcagcga gcctggatgg
     1501 ggttgttcca gatattcctg acagtgttgt ctcaaatgcc gtctgttatg accttgctta
     1561 ttcttatgga gagacgcctt ttctaaattg ggcaaaaaaa aatggctctg ctaaaaactt
     1621 acagggtttg ggtatgttgg tagaacaagc cgctgagtcc tttttcatct ggcggggagt
     1681 gaggcctaga acctctgagg ttgtagataa actccgatct gagctactaa cctaatcttt
     1741 gcaccagact gcgtaaacag ttgagtagtt ttctgcaatc tcagagaacg caagcgctgc
     1801 ttcaaacaat tctttttgtt tttgttcgtt tttaggtttt tctttgtcga gtctctcaac
     1861 ttctttaaac aagtaatctg cgatgcccaa cgctcctcct atgccttctt tggtttcatt
     1921 acaggcgttc tcatcaagca aatcatgatc acctgcaaat aacagagctc ctggcagact
     1981 caataaacaa atcaaaatac tttttttcat actaatatct ccagtcacta ctaataaaaa
     2041 gattacttac attatactgc tagcatatat gaaaattgca agctggaacg taaacggcat
     2101 cagggccaga gtagaacacg tcaaacattg gttaacgata tctcgtcctg atattttagc
     2161 attacaggaa acgaaagtgg tcgatgatct tttccctttt gatgcttttg aatccattgg
     2221 atatcaagcg gtagtgtttg gccaaaaagc atataatggc gttgctttac taacaaaaaa
     2281 caaagccccc aacatcaccc gaggtatgga tggttaccaa gacgagcaag cccgtgtaat
     2341 ctcagcagat tataaaggta ttcgaatcat tgatgtttat gttccgaatg gacaaagtgt
     2401 gggttccgag aaatttgcct acaaaattga gtggttttca catcttcatc ggttgataaa
     2461 acagtctttt caagccaata aaaatcttgt ggtgttgggt gattttaata ttgccccaga
     2521 agatatagat gtttacgacc cggaagtatg gaaagacaag gtgctctgca gtgatcaaga
     2581 aagaacatgc ttgcggtctc ttttatctct aggcttatcc gatagcttta ggctatttga
     2641 acaactagaa gagcaataca gctggtggga ctatagagca gcagct
//
LOCUS       DASCTH010000219         3634 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000367623, whole genome shotgun sequence.
ACCESSION   DASCTH010000219 DASCTH010000000
VERSION     DASCTH010000219.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000367623"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            48..905
                     /gene="folD"
                     /locus_tag="QGH35_04655"
     CDS             48..905
                     /gene="folD"
                     /locus_tag="QGH35_04655"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HJL81857.1"
                     /translation="MTAKIIDGKQIANEILLEVKETISKATMKGEKPPGLAVIQVGEN
                     SASKIYIKNKRIACSKVGFKSFNYDLPEQTSEKELIELVVSLNSNPEVNGILVQLPLP
                     PQINETIIIETIEPIKDVDGFHPYTIGRLMQRIPILRPCTAIGVMTMLDSIGADPIGK
                     HVVIVGASNLVGRPLALEFLLRGATTTVCHRFTENLEHFVRQADILAVAVGKPNVVLS
                     EWIKKDAIVLDIGINRNKKGNITGDVDFEIAKEKASWISPVPGGVGPMTVAMLIKNTL
                     LASKLDLES"
     gene            930..1505
                     /locus_tag="QGH35_04660"
     CDS             930..1505
                     /locus_tag="QGH35_04660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008984416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJL81858.1"
                     /translation="MRTVINKLLIIFIACLWTLPQSIASENYPQVEMVTSSGTFVLEL
                     DRNRAPVTVENFLNYVKEGFYKDTIFHRVIEGFVIQAGGYTKDFEEKETKPEIINESG
                     NGLINQRMSIGMARNNLPHTGSSQFYINLVNNYSLDPMPTRWGYAVFGDVIDGMDVVD
                     AIGHSATELNQGFQDVPVAPIIILEARIIGE"
     gene            1502..2236
                     /locus_tag="QGH35_04665"
     CDS             1502..2236
                     /locus_tag="QGH35_04665"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HJL81859.1"
                     /translation="MKKLFISDLHLHESKPEVTFLFKHFLNGLLTEPESKPELYILGD
                     LFDFWIGDDYEQLLYSEIIQQLRMLTDAGIKTYLIHGNRDFLIGSEFLSRTGIELLED
                     PTILSIHNKRVMLSHGDLFCIDDTEYQEYKKMVRNRQWQQDFLSLTVDKRLQILGETR
                     DASIQSQQNKSIEIMDINQEELIAKIQDNNINILIHGHTHRPNQYKIKIGNRTCLRLV
                     LGEWTASSVKIIEWIETEPKLIDLIN"
     gene            complement(2229..3626)
                     /gene="purF"
                     /locus_tag="QGH35_04670"
     CDS             complement(2229..3626)
                     /gene="purF"
                     /locus_tag="QGH35_04670"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007158654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HJL81860.1"
                     /translation="MCQRKSNGLVADVFTNKHMLQLRGNIGIGHVRYPTAGSLSEVEA
                     QPFYVNAPFGICLAHNGNLVNAEQLRNHLELIDRRHLNTESDTEALLNLFAHELQKIY
                     IKDLTPKEIFQAVRGVFEKSSGGYAVISMILNEGLVAFRDPNAIRPLVIGTRESNQGT
                     EYMIASESVALDVLGYTILRDVLPGEAIYIDTDGNFYSHQCVQARSYTPCIFEFVYFA
                     RPDSVIDGMSVYKSRLRMGELLAQNIIEKGLHHDVDVVIPIPDTSRTSAIQVAHHLGV
                     KYREGFIKNRYIGRTFIMPEQATRKKSVRQKLNPIGLEIKDKNILLIDDSIVRGTTSK
                     QIIQMARKAGAKRVFFASAAPPVKYPNVYGIDMPATSELMASRKNEDEMAKAIGADWL
                     VYQTLEDLIEAVTYEETGIEAFETSCFSGEYITNDITEHYLNIIELKRNDAAKAQQKE
                     MRKQLEINDPSIAIN"
BASE COUNT         1148 a          696 c          667 g         1123 t
ORIGIN      
        1 aaacctttgc tatcttggct taccccattt ctacaaagtg attacatatg acagcaaaaa
       61 taattgatgg taaacaaatt gcaaatgaaa ttctcttaga agtcaaagag accatttcaa
      121 aagcaacaat gaaaggcgaa aagccgccag gattagcagt catccaagtg ggtgaaaatt
      181 cagcatctaa aatttacata aaaaataaaa gaattgcctg tagtaaagtt ggcttcaagt
      241 cattcaatta cgatttgccc gaacaaactt cagaaaaaga gttaattgaa ttggtggttt
      301 cactcaacag taatcctgag gtcaatggta ttctggttca gcttcctctt cccccacaaa
      361 tcaatgaaac tattattata gaaacgatag agccaataaa agatgttgat gggtttcacc
      421 cttatactat cggtcgatta atgcaacgaa taccaattct gaggccctgt actgcgattg
      481 gagtcatgac tatgcttgat tctataggtg ctgaccctat aggcaagcat gtcgtaatag
      541 ttggtgcatc taatttagtg ggtaggcctc tagctttgga attcttgctt agaggagcaa
      601 caacgactgt ttgtcatcgt tttacagaaa atttggaaca ctttgtccgt caagctgata
      661 ttttggcggt agcagtaggc aaacccaatg tcgttctcag tgaatggata aaaaaagatg
      721 ccatagtgct tgatatcggt atcaatagaa ataaaaaagg aaacattaca ggtgacgttg
      781 attttgaaat agccaaagaa aaagcttctt ggatctcgcc tgtacccggt ggtgttggac
      841 cgatgactgt tgcaatgctg ataaaaaaca ctctacttgc ctctaaatta gatttagaat
      901 cataaaaata gaagataaag gttcaaattt tgaggacagt tattaacaaa ttattgatca
      961 tattcattgc ttgtctctgg actttgcccc agagtattgc atcggagaat tatccccaag
     1021 ttgaaatggt caccagttca ggaacatttg ttttggaact ggatagaaat cgagctcctg
     1081 taactgtaga aaattttcta aactatgtga aagaaggttt ttataaagat actatttttc
     1141 acagagtcat tgaaggcttt gttattcaag caggaggtta tacaaaagat tttgaagaaa
     1201 aagaaactaa acctgaaatc atcaatgaat cgggtaacgg tcttataaat caaagaatga
     1261 gcattggtat ggctcgtaac aacctacccc acactggcag ttcacagttc tacatcaatc
     1321 ttgtcaataa ttattccttg gatcccatgc ctactcgatg gggttatgcg gtctttggag
     1381 atgtaattga tggcatggat gtcgttgatg ctattggtca cagtgccaca gaattaaatc
     1441 aaggtttcca agatgttcct gtggctccca ttatcatttt agaagcaaga ataatcgggg
     1501 aatgaaaaaa ctatttattt ctgatctcca tcttcacgaa tctaaacctg aagtaacctt
     1561 cttatttaag cattttttaa atgggttact aaccgaacct gaatccaaac cggaactcta
     1621 tattctaggt gatctttttg atttttggat tggtgatgat tatgagcaat tactttattc
     1681 tgaaataatt caacaactta gaatgttgac agatgcaggc attaaaactt atcttataca
     1741 tggcaacaga gattttctta tagggagtga gtttttatct cgaaccggta ttgaattact
     1801 cgaagatcca acgattcttt caattcataa taaaagagtt atgctcagtc atggtgatct
     1861 attctgtatc gatgacacgg aataccaaga atataaaaaa atggtaagaa atcgacaatg
     1921 gcaacaggat tttctcagct taactgtaga caaacgactg caaattttgg gtgaaacaag
     1981 agatgcaagc atccaatctc aacaaaacaa atcaattgag attatggata tcaaccagga
     2041 agagctgatt gctaaaattc aagataataa tatcaacatt ctgatacacg gccatactca
     2101 tcgaccaaat caatataaga tcaaaattgg aaacaggaca tgtctacggc tggtattggg
     2161 tgaatggaca gcttcaagtg taaaaatcat tgagtggata gagacagaac ctaaattaat
     2221 tgatctaatc aattaattgc aatagatggg tcattgatct ccagttgttt tctcatttct
     2281 ttctgttgag cttttgcagc atcgtttctt tttagctcaa taatattaag gtagtgctca
     2341 gtaatatcat tggtaatata ttctcccgag aagcaagatg tttcaaatgc ttcaatacca
     2401 gtttcttcat aggtcaccgc ttctattaaa tcttccaaag tttgataaac taaccaatct
     2461 gcccctattg cttttgccat ctcgtcctca ttttttctac ttgccattaa ttcactggtt
     2521 gccggcatgt ctatcccata gacattaggg tatttaactg gtggtgccgc tgaggcgaaa
     2581 aatactctct tagccccggc cttccttgcc atctgaatga tctgtttaga agttgtacct
     2641 ctgacaattg aatcatcaat gagcagaata tttttgtctt tgatttcaag tccaatgggg
     2701 ttcagtttct gcctaacaga tttttttctt gttgcttgtt caggcataat aaaagttctt
     2761 ccaatgtatc gattttttat aaacccctcc ctgtatttaa cccccaagtg atgtgcaact
     2821 tgtatagcac tggtgcgact ggtatcaggt attggtatga ccacatccac atcatgatgc
     2881 agcccttttt cgataatatt ctgtgccaaa agctcgccca ttctaagtct ggatttataa
     2941 accgacatac catcaataac tgaatctggc ctggcaaaat aaacaaactc aaaaatacaa
     3001 ggtgtatagc ttcttgcttg aacgcactga tgtgaataaa agttaccatc ggtatcaatg
     3061 taaattgcct ctccaggtaa tacgtccctc aaaatggtgt atcctaaaac atccaaagcc
     3121 acactttcag aagcaatcat gtattcagtg ccttgattgg actctctagt tccaattacg
     3181 agcggtctaa tagcgtttgg atctctgaaa gcgactaagc cttcatttaa aatcatcgat
     3241 atcactgcat agccacctga tgatttttca aaaactcctc tgacggcttg aaaaatctct
     3301 tttggtgtca aatcttttat gtaaatcttc tgcaattcgt gtgcaaaaag attcagtaga
     3361 gcttccgtat cagattcagt attcagatgc cttctatcta tgagttctaa gtgatttctt
     3421 agttgttcag cattcaccaa atttccatta tgggcaaggc atatcccaaa aggtgcatta
     3481 acgtaaaacg gctgagcttc aacttcactt aatgaccctg cagttggata gcgaacgtgt
     3541 ccaatgccta tattgccccg caattgcaac atgtgtttat tagtaaaaac atcagcaacc
     3601 aagccgttgc ttttcctttg acacaagacg ccat
//
LOCUS       DASCTH010000220         2040 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000367815, whole genome shotgun sequence.
ACCESSION   DASCTH010000220 DASCTH010000000
VERSION     DASCTH010000220.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000367815"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="QGH35_04675"
     CDS             complement(<1..908)
                     /locus_tag="QGH35_04675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81861.1"
                     /translation="MSSIGQSLAKGVNKVDNQHPWPSPAQGWYAVSILVIAYIFSFID
                     RTIIALLVEPIKQDLQISDFGIGLLQGLAFAIFYALVGIPIGRLADRYSRRRIITAGI
                     FLWSLMTAACALAKSFMSLFLVRVGVGIGEAALSPSAYSMISDLFPREKLGRATGFYS
                     AGAFLGLGIAFLAGGWVIQILSSAGSIDLPLVGVMKPWQMTFIVVGLPGVLVAFLMMT
                     VKEPVRRGKLTGHDKGIPLQLVVSYVKANGKLFTMLFSGFALIAVPLTTFITWGPAYM
                     ARVHNFSMAETGFMLGMILLFLSPAGA"
     gene            complement(985..>2040)
                     /locus_tag="QGH35_04680"
     CDS             complement(985..>2040)
                     /locus_tag="QGH35_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81862.1"
                     /translation="LISILEFSQSQANLISSAEIAGMALSSLLSILWIGRFNWRKVAL
                     CALLAIVLGNLISTYITDFQLLVSIRFLTGLVGHGTAFALGIAAIGSTSEPDKNFGFT
                     IASQVVMGALTLFFAPKLIAAYGIAGMCFPAAILACIAMTLTSRLAQSGGVQAHGSDA
                     NQTTGLSFIGKYGLPIAGLLVMIAWQMGVGPFFNNLFPFGISINLDPSEIGTALFLST
                     GLSIIGPLSASALASKVKRGIPVCLALAVQLLVVLSFQGEMTWAGFAIRAILFQTAWN
                     FTGPFLMGMIATMDNTGRFSALIPASQLGGIAIGQAIIASLVQGNNLALINYFCAIVI
                     FLSALIFIFISGKINRN"
BASE COUNT          686 a          459 c          399 g          496 t
ORIGIN      
        1 gctcctgcag gcgataaaaa taataatatc atcccgagca taaaacctgt ttctgccatc
       61 gagaaattgt gtacacgtgc catataagcg ggaccccaag taataaaggt agttaaagga
      121 acagctatta atgcaaaccc agaaaacaac atagtgaaca atttaccgtt ggctttcaca
      181 taactaacta ctagttgtaa agggattcct ttgtcatgac cggtaagctt gccccgtctt
      241 actggttcct taacagtcat catcaggaag gccaccaaca cacctggcag cccgacaaca
      301 atgaaagtca tttgccaagg tttcataaca ccgaccaatg gtaaatcgat actacccgcc
      361 gaggagagta tctggataac ccagccaccc gctaaaaagg caatacccag accaagaaat
      421 gctcctgctg aataaaaccc agtcgcacga cctagcttct ctcttgggaa aagatccgag
      481 atcatagaat aagccgacgg tgataatgct gcttctccaa taccaacacc aactcgtacg
      541 agaaataagc tcataaaact cttagcgaga gcacaggcag cggtcattaa gctccataga
      601 aaaataccag ctgtaataat gcgacggcga ctataacggt ccgctaaacg tccaattggg
      661 atcccaacca gcgcatagaa aattgcaaat gctaatccct gtaacaatcc gataccaaag
      721 tctgaaattt gtaaatcttg tttgataggt tcgactaata aggcaatgat ggttcgatca
      781 ataaaagaga aaatataagc aataacgaga atgctgacag cataccagcc ttgagcagga
      841 ctaggccatg gatgctgatt atccacttta ttaactccct tagctagcga ttggccgata
      901 ctactcatta cagtaactgc ctaatgattc ccatcatttg ggtgattttt ttatcaagag
      961 ccccattttt cttagaacag gcaatcaatt acgattgatc tttcctgaga taaaaataaa
     1021 tattaaagcg gaaagaaaga ttactattgc acaaaagtag ttaattaaag caagattgtt
     1081 gccttgtacc aaggaagcta tgatggcttg accgattgca atcccgccca attgagatgc
     1141 tgggatcagt gcactaaatc tgccagtgtt atccatagta gcaatcatgc ccattaaaaa
     1201 tggacctgtg aaattccaag cagtttgaaa aaggatggcc ctgatggcga agcctgccca
     1261 ggtcatttct ccttgaaatg acagtacgac taataactga actgccaacg ccaaacatac
     1321 agggatgcct cttttaacct tagatgccag tgccgaggca ctcaaaggac caataatact
     1381 cagtccagta ctcaaaaata aagcagtgcc aatttcagat ggatcaaggt taattgagat
     1441 cccaaaagga aaaaggttgt taaagaaagg gccaacaccc atttgccagg ctatcatcac
     1501 tagaagtcca gcaatgggta gtccatattt tccaataaaa ctcaatcccg ttgtttgatt
     1561 ggcatcactt ccatgggctt ggactcctcc tgattgagct aatctagaag ttaaggtcat
     1621 tgcaatacag gctagaatcg cagctggaaa acacattcct gcaatgccat atgctgcaat
     1681 cagttttggt gcaaaaaata gagttaatgc acccataaca acttgggatg cgatagtgaa
     1741 gccaaaattc ttatctggtt ccgatgtgct gcctatggca gcaattccta gggcaaaagc
     1801 agtgccgtgc ccaaccaatc ccgtgagaaa tcgaatgcta actaagagtt gaaaatcagt
     1861 gatatacgtt gaaattaaat tacccagaac aatcgccaat aaagcgcata aagctacttt
     1921 tctccagtta aaacgaccta tccatagaat gcttagaagg gatgataatg ccatgcctgc
     1981 aatttctgcc gaggaaatca agtttgcttg agactgacta aactcaagta tagaaattaa
//
LOCUS       DASCTH010000221         1775 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000374845, whole genome shotgun sequence.
ACCESSION   DASCTH010000221 DASCTH010000000
VERSION     DASCTH010000221.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000374845"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(101..1036)
                     /locus_tag="QGH35_04685"
     CDS             complement(101..1036)
                     /locus_tag="QGH35_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HJL81863.1"
                     /translation="MNSKQQSFIECIHPINWLIVLGLVCTRIIVLLPFRIILTIGQAM
                     GLLLYRISSSRLQIVRKNIELCFPTLSATEQNHLVKQHFISLGIGFFEVGMARWKSNQ
                     SLKKIIEIKGIEHLNRAVEKNRGVILMSAHFTMLEISALIGRNEIVQEDLPPMVGMFR
                     LGKNSVINHFFRNTRLRSTESLVTKFEVKDLIRALKDKKLVWYASDQSFTGKNAISVN
                     FFNHPAMTNPSIIRIAKISGSPVLPYFPSRLANGKGYILEVLPEIESFPSEDANNDMK
                     KIHALLEQHILQNPEQYYWVHRRFKTAAGNKNLYQ"
     gene            1128..1520
                     /gene="msrB"
                     /locus_tag="QGH35_04690"
     CDS             1128..1520
                     /gene="msrB"
                     /locus_tag="QGH35_04690"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005279312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HJL81864.1"
                     /translation="MSKDSNDHSDLGHLNSDQVFVTQEKGTEMPFSGQYLNNKETGTY
                     LCVCCSNVLFSSSTKFDSGTGWPSFFDIQHRDSIKIVSDYSHGMNRKEVLCANCEAHL
                     GHLFKDGPEPTGLRYCINSLSLDFEPEN"
     gene            complement(1517..>1775)
                     /locus_tag="QGH35_04695"
     CDS             complement(1517..>1775)
                     /locus_tag="QGH35_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipid-binding protein"
                     /protein_id="HJL81865.1"
                     /translation="GINDYTGFMAGDPEMEGNYFGYEGPCPPWNDEIAHHYRFKLFAC
                     DFNVCPVAGTFTGEDVLKAAQGHILSETELIGLYSLNPNLS"
BASE COUNT          542 a          336 c          324 g          573 t
ORIGIN      
        1 acacaatgaa cataatatta aaactgtatt aaatactttt ctcattgtta atgttgcttt
       61 caagcagtcc ataatatttt attagtgtaa tgtttttata tcattgatat agatttttat
      121 tacctgcagc tgtcttaaat ctccggtgaa cccaataata ttgttctgga ttttgcagga
      181 tgtgttgctc cagcagtgca tgtatctttt tcatatcatt gttggcgtct tcacttggaa
      241 aagactcaat ttctggcagt acttctaaaa tatagccttt cccattagca agtcgagatg
      301 ggaaataagg caatactggg gatccggata ttttcgctat tctaatgatg cttggattgg
      361 tcattgccgg gtggttaaaa aaattaacag aaattgcatt tttccctgta aaactttgat
      421 ctgaggcata ccagactaat tttttatctt tcaaagcccg aatcagatct ttgacctcaa
      481 atttggtcac taaactttct gtagacctta atcgagtatt acgaaaaaaa tgattaataa
      541 cagaattttt tcccaatcga aacataccta ccattggagg taagtcttct tgtacaattt
      601 catttctgcc aatcaaggca ctgatctcca acatggtaaa atgggcactc attaaaatca
      661 ctcctcgatt tttctcaacc gcacgattga ggtgctcgat gcccttaatt tcaattatct
      721 tctttaggct ttggttggat ttccatcgag ccatgcccac ttcaaaaaag ccaatgccaa
      781 gcgatataaa atgctgtttc actaagtggt tttgttctgt tgctgacaag gttggaaaac
      841 acaattcaat atttttcctg acaatctgca atctggatga tgatattcga tacagcagca
      901 atcccatagc ttgaccaata gttaaaatga ttctaaaagg aagcagcact attattctag
      961 tgcacaccaa acccaaaaca atcagccaat ttattggatg aatgcattca ataaatgatt
     1021 gttgttttga gttcattgtc atagtttaat cataatctag attatagata caaatagacg
     1081 ggataaagct gcctttgaaa acaatacaaa tggtttagaa tggagggatg agtaaagatt
     1141 cgaatgatca tagtgatctg ggtcatttaa actctgatca agtgtttgtt acccaagaaa
     1201 aaggtacgga aatgcccttt agtgggcaat atctcaataa caaggagaca gggacgtatc
     1261 tatgcgtttg ttgtagcaat gttttattca gttcatcaac caaatttgat tctggaaccg
     1321 gttggcctag ttttttcgac attcaacata gagattctat aaaaattgta tccgactaca
     1381 gtcatggaat gaatcggaaa gaagtacttt gtgctaactg cgaagctcac ctcgggcatt
     1441 tgtttaagga tggtcctgaa ccgacaggcc tcaggtattg catcaattcg ttatccctcg
     1501 attttgaacc ggaaaattaa cttagatttg ggttaaggct atacaatcct atcaattccg
     1561 tctcagagag tatgtgtcct tgagcggctt tcaagacatc ttcaccggtg aaagttcctg
     1621 ctactggaca aacattaaag tcgcatgcaa acaatttaaa tcgataatga tgggctattt
     1681 catcattcca aggggggcat ggtccttcat agccaaaata attgccctcc atttctggat
     1741 ctcctgccat aaagccggtg taatcattaa tgcct
//
LOCUS       DASCTH010000222         3192 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000388048, whole genome shotgun sequence.
ACCESSION   DASCTH010000222 DASCTH010000000
VERSION     DASCTH010000222.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000388048"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="QGH35_04700"
     CDS             complement(<1..700)
                     /locus_tag="QGH35_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HJL81866.1"
                     /translation="MTKVKIEKKIIDCKVDSTKAHDQSKNIMKEYPEGGKEKETPESK
                     VVRMHERLERPVMLRGSTYKIKPPISDHAMYITINDIVLNPNTDHEKYRPYEIFINSK
                     NLDHYQWIVALTRVMSSVFRKGGDVAFLVEELKAVFDPKGGYWQPGGTGVFMPSIIAE
                     LGFVVEKHLQSIGLIPEERLDEYQKQLLDEKRQEFENRNKQQDAFVKKSFPEGAQMCN
                     HCNTVATVMMDGCLT"
     gene            complement(724..2874)
                     /locus_tag="QGH35_04705"
     CDS             complement(724..2874)
                     /locus_tag="QGH35_04705"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HJL81867.1"
                     /translation="MKSAPKKSEHETLEVEYQTASIDIWDKKYRLKELSGKPIDLDID
                     DTFKRVAKALSELEDPEQQEKWEQEFLWTLRSGAIPAGRIMSNAGAGEHKPATSTINC
                     TVSGSIHDSMIGILEKVTEAGLTLKAGCGIGYEFSTLRPKGAYVTGAGAFTSGPLSFM
                     DIYDKTCFTVSSAGGRRGAQMATFDVSHPDVEDFIKAKQEDGRLRQFNLSLLITNEFM
                     EAVTKDEDWSLAFPLDPRLPESAAIDLEDPTKVLWKPWTVTEGYITNEEGLVACKVYK
                     VIKARQLWDLIMASTYDYAEPGFILIDRVNEMNNNWFDENIRATNPCGEQPLPEYGSC
                     LLGSVNLTRFVQNPFSQAVSFDWDCFRKVVSIFTRMLDNVVEINALPIQQQRDEILSK
                     RRHGMGFLGLGSAMTMMGMKYGSEASLEFTDRVSKELAMTGWKVALELAKEKGPAPIL
                     LKEFCVTKDMLKKKPEMIDDGWQIGDKIPGRILHAKYSSYMQQIAEIEPELVEHLSEI
                     GARFTHHTSIAPTGTISLSIGNNVSNGIEPSFAHHYFRNVIREKKKTKEKIDVFSYEL
                     LSYKKLVNPQIDTESGGELENQLPDYFITAEDITPKEHVDVQSVAQKWIDSSISKTAN
                     VPTDYRFEDFKDIYLYAHEKGLKGCTTFRFNPEAFQGVLVKEKDLEKTFYTFVLDDGK
                     EVQVAGNEEIEYDGEVHTAANLFDALKEGYYGKL"
BASE COUNT          910 a          675 c          507 g         1100 t
ORIGIN      
        1 aagtcaaaca gccatccatc ataactgttg caacggtatt acagtggtta cacatttgag
       61 ctccttcagg aaaagatttt tttacaaatg cgtcttgctg tttgtttcta ttctcaaatt
      121 cttgcctttt ttcatccagg agctgctttt gatattcatc caatctttct tctggaatca
      181 agccaatgga ttgaaggtgt ttttccacaa caaaacctaa ttctgcaata atggatggca
      241 taaaaactcc agttcctcca ggttgccaat aaccaccttt ggggtcaaaa actgctttta
      301 actcttccac caaaaaagct acatctccgc cttttcgaaa cacggaagac ataacccttg
      361 tgagtgcaac tatccattga taatgatcta ggttttttga attaatgaaa atttcatagg
      421 gcctgtactt ctcatgatcc gtatttggat ttaacacgat atcattaatt gtgatataca
      481 ttgcatggtc tgatattggg ggtttaattt tataagttga gcccctgagc atgaccggtc
      541 tctctaggcg ttcatgcatt ctaactactt ttgattccgg tgtttctttc tcctttcctc
      601 cttcaggata ctctttcatt atatttttag attgatcatg agctttagtc gagtcaacct
      661 tacagtcgat aatttttttt tctattttca ccttcgtcat tagcaaacca tccttatttt
      721 gtactataat tttccataat agccctcttt caatgcatca aaaagattgg ctgcggtgtg
      781 tacctcaccg tcgtactcaa tctcttcgtt acctgccacc tgtacttctt ttccatcatc
      841 caaaacaaag gtataaaagg ttttctcaag atctttctct ttgactaaca ctccctggaa
      901 agcttctgga ttaaaacgga atgttgtgca tccttttaat cctttttcat gtgcataaag
      961 ataaatatct ttgaaatctt caaatctata gtccgtaggt acattagcag tttttgaaat
     1021 agaagaatcg atccattttt gtgccacact ttgaacatca acatgttctt taggggtaat
     1081 gtcttcagca gtgataaagt aatctggcaa ttgattctcc aattcaccac ctgattcagt
     1141 atcaatctgt gggttgacca gttttttata cgacaacaat tcatatgaaa aaacatcaat
     1201 tttttctttg gttttctttt tttccctaat gacattccta aaataatgat gtgcaaaact
     1261 tggttcaatg ccattactga cattgttacc aatcgacaac gaaatggttc cagtaggtgc
     1321 gatggatgta tgatgcgtaa atcttgcacc aatttccgat aagtgctcaa ccaattcagg
     1381 ctctatttct gcaatttgtt gcatgtaact actgtatttt gcatgcaaaa ttctaccagg
     1441 tatcttatcg cctatttgcc aaccatcatc gatcatttct ggttttttct tgagcatgtc
     1501 tttggtgacg caaaattctt ttaataaaat aggtgcagga cctttttcct tagctagttc
     1561 taaagcaact ttccagcctg tcatagccag ttccttgcta acccggtctg taaactcaag
     1621 tgaagcttca ctaccatact tcatgcccat catggtcatg gctgatccca gtcctaaaaa
     1681 tcccatgccg tgtcttctct tactgaggat ttcatctctt tgttgctgaa tgggtaaggc
     1741 attaatctcc acaacattat ccagcattct cgtaaaaatg ctgacgactt tcctgaaaca
     1801 atcccagtca aaagatacag cttggctaaa tggattctgc acaaaacgag ttaggttaac
     1861 tgagcccaac aaacaagaac cgtactcagg caaaggttgt tccccacatg gattagtcgc
     1921 acgtatattt tcatcgaacc agttattatt catttcatta actctatcta taagaataaa
     1981 tcctggttca gcataatcgt atgttgaggc cataataaga tcccatagtt gtctggcttt
     2041 tataacttta taaaccttac aagcgaccaa accttcttca ttagttatat aaccttcagt
     2101 gacagtccat ggtttccaaa gaacttttgt tggatcttcc agatcaatag ctgcagattc
     2161 tggcaatctt gggtccaagg gaaaggcaag agaccaatct tcgtcttttg tcactgcttc
     2221 catgaactca tttgtgatca atagtgaaag attgaactgc ctcaatctcc cgtcctcttg
     2281 tttggcttta ataaaatctt caacatctgg atgactaaca tcaaaagttg ccatttgagc
     2341 tccccttctc cctcctgcag aagaaacggt gaaacacgtt ttatcgtaga tatccataaa
     2401 tgacaatggt ccagaagtaa aagcacctgc gcctgtaaca taagcacctt ttggtcttaa
     2461 agttgaaaac tcataaccga ttccacagcc tgcctttaat gttagacctg cctcagttac
     2521 tttttccaat attcctatca ttgaatcatg tatcgaacca gaaacagtgc aattaatggt
     2581 tgaggttgcc ggcttatgtt ctcctgcgcc agcattagac ataattcttc cagcaggaat
     2641 cgctcctgaa cgtaaagtcc acaaaaactc ttgttcccat ttttcttgtt gttctggatc
     2701 ttctaattct gataaagcct tagccactct tttaaaggtg tcatctatgt ccagatcaat
     2761 cggttttcca gaaagttctt tgagtcgata ttttttatcc caaatatcaa tggaagcagt
     2821 ttggtattcg acctctaaag tctcatgttc tgacttttta ggtgccgatt tcatttgtat
     2881 ctaaggttcc cctgttctag attgatattg cgagttttgg tctaaaatca caaaaaagtg
     2941 aagaaaatac tgcacaagat atagtgtttt gacaataata gattattgcg catttcagcg
     3001 tgttgtcaag aattatttga gatcagtaat tattatcatt tttttatata tttcaatatc
     3061 ttatgagttt tatactagac aaataattga atttttttgc aaaaattaac taaattacat
     3121 gaaaacacct accacaatat gtagtgtttt agatattcac ctataattca atgtttatca
     3181 acaaattatt aa
//
LOCUS       DASCTH010000223         5867 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000399741, whole genome shotgun sequence.
ACCESSION   DASCTH010000223 DASCTH010000000
VERSION     DASCTH010000223.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000399741"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="QGH35_04710"
     CDS             <1..270
                     /locus_tag="QGH35_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJL81868.1"
                     /translation="SFENEDLETIAQQANDTSYGLSGSVWTQDISVAHKMAMLIDAGQ
                     VSINCHAAVDPAIPFGGNKQSGWGREFGKEGLEPYLKTKATSVIF"
     gene            complement(282..992)
                     /locus_tag="QGH35_04715"
     CDS             complement(282..992)
                     /locus_tag="QGH35_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL81869.1"
                     /translation="MPTVLVTGANRGIGYEHVAQYAQKKWTVIACTRQPEKAIELLQL
                     QDQYGTNFVIEELEVTNHKQIDELANKYKNTAIDILINNAGTGGPVGMPTAMDYQKID
                     NMDYQIWREILEVNLLAPFKVATSFYKQISISDKKILIMMSSDLGSVYQNNFGGLYSY
                     RTSKSGLNVIAKGMSNEWKNIIVVSLAPGWCRTHLGGAEAEIDPGDSVEQQQKMFESL
                     TASDNGKFLDRFGNDVPW"
     gene            1079..1663
                     /locus_tag="QGH35_04720"
     CDS             1079..1663
                     /locus_tag="QGH35_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013960142.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HJL81870.1"
                     /translation="MSQPNYISALDLHQNPEIKNLKKETQDYFDECIEKLGFVPNVLL
                     AYAFDETKLDQFMGFYNNLMLDDSGLSKLEREMIAVVVSSYNRCYYCMVAHGAAVRHI
                     SKDPMLGEVLCMNYKVAELSSRHRIMLDFAWKMSESPMLIEDADRQNLRDNGFDDKDI
                     WDISAVASFYNMTNRMASAINLMPNPGYHKKARG"
     gene            1728..2573
                     /locus_tag="QGH35_04725"
     CDS             1728..2573
                     /locus_tag="QGH35_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HJL81871.1"
                     /translation="METTELTNSITQIFTDLAGPLATFGKRILFAVLIIVVGRWVVKL
                     LLRIINTALEKTTIEETVRIFVGNLLRTLFMIVVFIAAINQLGIETTSIIAMLGAAGL
                     AIGLALQGSLANFAAGILIVTFRPYKVGDYIQAGSASGTVLDIQIFSTVLKTPDNKVI
                     VIPNGTIMDGSITNYSEQPTRRVDIVASCGYEDDIDKVKQLLTDIINQDDRVLDEPEP
                     LVALSELADNSINFVVRPWVNSSDYFPVYYSLLEQIKKRFDKEGISIPYPQRDIHIYN
                     HENKN"
     gene            complement(2601..4478)
                     /locus_tag="QGH35_04730"
     CDS             complement(2601..4478)
                     /locus_tag="QGH35_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HJL81872.1"
                     /translation="MSTKIIQTNNKPFKRVKLDPITLDVIENSMFSARWEMDAVLFRT
                     AMSPGIREQGDEFPMIANLDGKMVVGQFGSFIYGFMEAYDGTMEEGDMFLTTDPYSCN
                     GAISHVNDWLLLRPIYKDGRLISYAAMFGHMTDVGGKVPGSLPTDASQIFEEGIRVPP
                     IKIYKNDELQEEILELILNNCRLPHWNRSDFNAIVAAIRMAEKRVLEMAERFGDDIYY
                     SALEELLDRNKTAMGKLITNTVPTEKQYFEDYICDDGLGMGPYKIKCAMWRQGKKVIF
                     DFEGTDPQSISSVNFYLNEEMFKMFCGVYMIMVFDPAIMFNDGFYDLMDVRIPEGTLL
                     KPLHPAALSCRTHALGRIFDILGGLLGQGSPDFLCAAGFSDSPHLMYSGYDKKGEWYQ
                     LYMIGFGGVPGKPMGDGPDGHSLWPSFTNVPNEFLESYFPLIIEEYYTVPDSGGAGHH
                     RGGNALCTTYRFLEPGEVSIHDDRWLTYPWGVNGGHPGARSTKTLVRSNGDTELLQSK
                     CDRIKVEEDDVLYHVTWGGGGWGDPLTRPAERVAFDVDAGLVTRKGAKKNYGVVLSKE
                     LKINNSATTKLRKEMAKARGKTKLFDKGFSSISELKKRCKAETGLEPPATPKFQKWMQ
                     A"
     gene            complement(4491..>5867)
                     /locus_tag="QGH35_04735"
     CDS             complement(4491..>5867)
                     /locus_tag="QGH35_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HJL81873.1"
                     /translation="QKGLKRRMNDVKLHILRSDGGLASAKASENSPINVLMSGPAGGV
                     SGAIWIAQQAGYNNLLTFDMGGTSTDVSLVLNGRAAQSRETTVGDVTVRASSVDVRTV
                     GAGGGSIAHVPELTGALRVGPQSAGAEPGPAAYDKGGVEPTVTDANVALGYMPPNVRL
                     GGDMKIRKDLAVDAVKTVADPLGLSVQAAAAGIYDIVNENMYGALRLVSVEKGFDPRD
                     FALIGFGGAGPLHANALGKLTNSWPVIIPPGPGVLCAYGDVTTTIQDEASMSFIRRFS
                     DTNKKEVVQIFTDLSKKASKTLDAEGVAKKNRTTTYQVDIRYHGQGLLLTVDFDLSEL
                     RRKGLNAIGNTFDEMHEQLFTFALPAEKELVNLRAVVRDKALNVKAPSVPRGNYNPSG
                     AFISKTKVFMEGKNRFASLYERSKLKQGNIIKGPAIVLEMDSTTVILPDHSGRIDKFG
                     NILITPDA"
BASE COUNT         1649 a         1219 c         1114 g         1885 t
ORIGIN      
        1 tctttcgaaa atgaggattt ggaaactata gcccaacaag ccaatgacac ctcctatgga
       61 ctatctggga gtgtttggac acaagatatc tcagttgctc ataaaatggc catgctgatt
      121 gatgcagggc aagtgagtat taattgtcat gcagctgtgg atccagctat cccatttggt
      181 ggcaataaac aatcaggttg gggaagagag tttggcaaag aaggcttgga gccctacctt
      241 aaaaccaaag ctacatctgt tattttttag actgggttta actaccaggg tacgtcgttt
      301 ccgaatcggt ctaagaattt tccattgtcg gatgcagtca gagattcaaa cattttttgt
      361 tgttgttcaa cgctgtctcc agggtcaatt tctgcttctg ctccgccaag atgtgttcga
      421 caccagcctg gtgcaagtga aacaacaatg atgtttttcc attcattaga catccctttg
      481 gcaataacat tcagtccaga cttactagtt cggtaggagt ataagccgcc aaaattattt
      541 tgatatacag agcctaaatc actagacatc atgatgagga ttttcttatc gctaattgaa
      601 atttgtttgt aaaaggatgt tgccacttta aaaggtgcaa gcaagtttac ctccaagatc
      661 tctctccata tctgataatc catattatcg attttttggt agtccattgc cgtaggcatt
      721 cctacaggac ctcctgtacc agcgttgttg attaaaatat caattgcagt gtttttatac
      781 ttatttgcaa gttcatcgat ttgtttgtga ttggtgacct caagttcttc aatgacgaag
      841 tttgttccat attgatcttg tagttgcaat aactctattg ctttttctgg ttgccttgta
      901 caggcgatga ctgtccattt cttctgagca tattgagcaa catgttcata accaatgccc
      961 cgattagcgc ctgttaccaa gactgttggc ataatctttc cctcgtattg ctattgactt
     1021 taaatagaac ataatatcaa aatagttttt atcattttca aaaaaaagag gtctaataat
     1081 gtctcaaccc aattatattt ctgcacttga tcttcatcag aatcctgaaa taaaaaatct
     1141 aaaaaaagaa acacaagact actttgacga atgtattgaa aaattaggtt ttgttcctaa
     1201 tgttcttttg gcttatgcat ttgatgaaac taaactggat cagtttatgg gtttctataa
     1261 caatcttatg ttggatgatt ctggtttgtc aaaattagaa agagaaatga tcgcggttgt
     1321 agtttcttca tacaaccgtt gttattactg tatggttgct catggagcag cagttagaca
     1381 tatttcaaag gatccaatgc ttggtgaggt tttatgtatg aactataaag tagccgaatt
     1441 atcttcaagg catcgaatta tgttagattt tgcatggaaa atgagtgaat ctccgatgtt
     1501 aattgaagat gcagatcgtc agaatttaag agataatgga tttgatgata aggatatttg
     1561 ggatattagt gcggttgcat ccttttacaa tatgactaat cgaatggctt ctgcaattaa
     1621 tttaatgcca aatccaggtt atcacaagaa ggcaagagga tagattgcta ttataatttg
     1681 ctataattct cagatataaa aaataaactc aaaatcatca ataattaatg gaaacaactg
     1741 aacttacaaa ttctataact caaattttta ctgatctagc aggtcctctg gcaacttttg
     1801 gtaaacgcat cctttttgca gttttaatca ttgtggtagg tcgatgggtg gttaagctac
     1861 ttttaagaat catcaatact gctcttgaga agacaaccat tgaagaaact gtgaggattt
     1921 tcgtaggcaa tcttctcaga accttattca tgatagttgt ttttattgct gcaattaatc
     1981 aattaggcat agagactacc tcaataattg caatgttagg agcagctggt ttggctattg
     2041 gtttggccct acaaggatca ttagcaaact ttgctgcagg aattctgatc gtaacttttc
     2101 gaccctacaa agtgggtgat tatattcaag caggttctgc atctggtacg gttttagata
     2161 ttcaaatttt ctcaaccgtc ctaaagacac ctgataacaa ggtgattgtc atacccaacg
     2221 gaacgatcat ggatggcagc atcaccaact attctgagca acctacacgc cgtgtggata
     2281 tagtcgctag ttgcggctac gaagatgaca ttgataaagt taagcaatta ttgactgata
     2341 ttatcaatca ggatgacagg gtacttgatg agccagaacc acttgtggct ttatctgagc
     2401 tggctgataa cagcatcaat tttgttgtca ggccttgggt taattcttct gattattttc
     2461 ctgtttatta ttctctattg gagcaaatta agaagagatt cgacaaagag gggatctcaa
     2521 ttccctatcc tcaaagggat atccatatct acaatcacga aaataaaaac tagcttcctg
     2581 attccttttt tacagtcggt ttaagcttgc atccattttt ggaactttgg agttgctgga
     2641 ggctcaagtc cggtttcagc tttgcaacgc ttcttgagtt cactaatact tgagaaccct
     2701 ttatcgaata gttttgtttt gcctcttgct ttagccatct cctttcttaa tttagttgta
     2761 gcagaattgt ttatcttcaa ttccttactg agtacgacac cataattttt cttagcacct
     2821 tttctagtta ctaagccggc atcaacatcg aaggcaactc tttcagcagg tcttgttaat
     2881 gggtcacccc atcctcctcc accccaagtt acgtggtaga gtacatcgtc ctcttcaact
     2941 ttaattcgat cacattttga ttggagtaat tcagtatctc cattgcttct taccaatgtt
     3001 tttgtactgc gtgcacctgg atgacctcca ttgacacccc aaggataagt aagccatcgg
     3061 tcatcatgta tagagacctc tccgggctct aagaatcgat aagtagtgca aagagcatta
     3121 ccacctcgat gatgtcctgc tccaccgcta tcaggtaccg tgtagtattc ttcaataata
     3181 agtgggaagt aactttctaa aaactcattt gggacattgg tgaagctagg ccaaagtgaa
     3241 tgaccatctg gaccatcacc cattggtttt cctggcactc ctccaaatcc aatcatatag
     3301 agctgatacc attcaccttt tttgtcatat cctgaataca ttagatgggg actatcagaa
     3361 aatccagcag cacagagaaa gtcaggagat ccttgaccta ggagaccacc tagtatgtca
     3421 aaaattctac ctaaagcgtg tgttctgcat gataaggcag ctggatgtag gggtttcaaa
     3481 agggtgcctt ctggaattcg aacgtccatc aaatcgtaaa atccgtcgtt gaacataatt
     3541 gctggatcaa aaaccataat catgtaaacg ccacagaaca ttttgaacat ttcctcattg
     3601 agataaaaat tgactgagct gattgattgt ggatcagtgc cctcaaagtc aaaaattact
     3661 tttttaccct gcctccacat ggcgcattta attttataag gacccatccc aagaccatcg
     3721 tcacagatat aatcttcgaa atactgtttt tcagtcggta ccgtatttgt gatgagttta
     3781 cccatggcag ttttatttct atccaaaagt tcttcaagag cagaataata gatatcgtca
     3841 ccaaatcgtt cagccatttc caagactcgt ttctctgcca ttcttatcgc agcaacgatg
     3901 gcattaaaat cacttctatt ccaatggggt agacggcagt tgtttaagat taactccaaa
     3961 atttcttcct ggagttcatc atttttataa atttttattg gtgggactct gattccttct
     4021 tcaaatattt gacttgcatc tgttggaagg ctacctggga ctttaccacc tacgtcggtc
     4081 atatgaccaa acatggctgc ataactgatg agtctgccgt ccttatatat gggtctgagc
     4141 aacaaccaat cattgacatg actaatggca ccattgcatg agtacggatc agtagtaagg
     4201 aacatgtcac cctcttccat agttccatcg taggcttcca taaaaccata aataaaactt
     4261 ccaaattgtc ctacaaccat ttttccgtct aagtttgcaa tcattggaaa ttcgtcacct
     4321 tgttcccgaa tacctggaga cattgccgtt ctaaatagaa ctgcatccat ttcccagcga
     4381 gctgagaaca ttgaattttc tataacatcc aaggtaatgg ggtcaagctt gacccgttta
     4441 aagggtttat tattggtctg aattatcttt gttgacatga ggtactcctt ttaagcatct
     4501 ggggtaatca gaatattacc aaatttatca atccggcctg agtgatcagg caagattaca
     4561 gtggttgaat ccatttctag cacaatggct ggtcctttga ttatattccc ttgttttaat
     4621 tttgatcgtt catagagaga agcaaacctg tttttgccct ccataaaaac tttagtttta
     4681 gagatgaatg ctccagaggg attgtaattt cctctgggga cagatggtgc tttaacgttt
     4741 agagctttgt ctctgacaac tgctcttaaa ttcactaact ctttttctgc tggcagagcg
     4801 aacgtgaata gttgttcatg catttcatca aatgtgttgc caatagcatt gagtcctttt
     4861 cttctgagtt cactcaagtc aaaatcaact gttagcaata aaccttgtcc gtggtatcga
     4921 atgtccactt gataagtagt agttcgattc ttctttgcta caccctctgc atcaagtgtc
     4981 tttgaagctt tttttgagag atcggtaaat atttggacta cctctttctt gttggtgtcg
     5041 ctaaatcttc taataaagga cattgaagct tcatcttgaa tcgttgtcgt tacatctcca
     5101 taagcacaca ataccccagg tccaggtgga atgatgaccg gccatgaatt ggtaagtttt
     5161 ccaagtgcgt ttgcatgcag tggtcctgcg cctccgaaac caattaaggc aaaatctcta
     5221 gggtcaaatc ctttctcaac agaaaccaat cttagggctc cgtacatatt ttcattgaca
     5281 atatcataaa ttccagcggc tgctgcctga acagaaagac ccaaaggatc tgcaactgtt
     5341 ttaactgcat ccactgcaag atcttttcta attttcatat ctccacccaa gcgaacattg
     5401 ggtggcatat atccaagagc tacgtttgca tcagttacgg ttggctcgac tccgccttta
     5461 tcatatgctg ctggtccagg ttctgctccc gcactttgag gaccaactct taatgctcct
     5521 gtgagttcag ggacatgagc aattgatcca cctccagccc ctactgttct gacatccact
     5581 gatgaggctc gaactgttac atctccaaca gtggtttcac gactctgtgc tgccctgcca
     5641 ttcaagacta gtgaaacgtc agtagacgtt cctcccatgt caaacgtcaa cagattgtta
     5701 tagccagctt gttgagcaat ccagattgcg cccgatactc ctcctgcagg tccactcatt
     5761 aatacattaa taggcgagtt ttctgaggcc tttgcagaag ctagtcctcc gtctgatcta
     5821 aggatatgca gtttgacatc attcatacgc ctcttaagtc ctttttg
//
LOCUS       DASCTH010000224         2208 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000400210, whole genome shotgun sequence.
ACCESSION   DASCTH010000224 DASCTH010000000
VERSION     DASCTH010000224.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000400210"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            92..781
                     /locus_tag="QGH35_04740"
     CDS             92..781
                     /locus_tag="QGH35_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008219798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HJL81874.1"
                     /translation="MKHSFLALLVLITLLACQDSVNIYNKPDSIIDVEQLIPDAVFDL
                     RYASSNNFTGKQVSGYEAAKCLLDQEAAHALKKVQYSAKAKGLNLIIFDCYRPQRAVN
                     DFIKWLDQSEQVQLKDRYHPNLSKSELLGPYIAEKSEHTKGVTLDVSLTKQNVNGEYQ
                     QIEMGSSFDLFDPLSNTDNPSISSFHRNNRYLLKELMEGEGFIAYDMEWWHFTFNQSK
                     ESELYYDFVIR"
     gene            813..>2208
                     /locus_tag="QGH35_04745"
     CDS             813..>2208
                     /locus_tag="QGH35_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009020066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HJL81875.1"
                     /translation="MNKLITITRISISLLIIFILANPTLAALESDKKPNIVLVLMDNF
                     GYGEIGVYGGGVLRGAPTPNIDSIATDGFQLTNYNVEAECTPSRSALMTGRYGIRTRQ
                     RQDGPPRGVWYGITQWEITLAEMLSNEGYATGIFGKWHLGDTKGRYPTDQGFDEWNGI
                     PRSSDRAFWPDSNSFTPDSHPDADFAHVMSSFRGQEPKELEVYDRAKRATIDKDITDH
                     AIDFIKRKASSNQPFFAYLPYTQTHEPVDPHPDFFGTTGNGSFADVLAQTDAYIGELL
                     DTIEELGLKENTIFIFTSDNGREGVPRSFGFTGPWRGSMFSPYEGSLRVPFLIRWPGK
                     IPNQQVSNEIFHQIDVFPTLASFIGVDIPKDRIIDGVDQSDFLMGKTNKSARDSMVIY
                     IGNELFGAKWRNWKILIKEMDEDGYGIKNMAYPSVYNLIVDPKEEVPELNYLNDTWVD
                     FPLYQVLEDHEVSID"
BASE COUNT          700 a          394 c          448 g          666 t
ORIGIN      
        1 tatcagaaga ttcaagcagc cataaaataa aaaacctcag tcaaatttca caaattattg
       61 atggtggaaa gtctaggttt agaatagaat tatgaagcac tcattccttg ctttacttgt
      121 cttgattaca cttcttgctt gtcaagattc agtgaatata tacaataagc ccgattcgat
      181 cattgacgtt gagcaattga ttccggatgc ggtttttgat cttcgttatg cttcttccaa
      241 taattttaca ggcaaacaag tttcaggata tgaagcagca aaatgccttc tagatcaaga
      301 ggccgcccat gctttaaaaa aagttcagta cagcgcaaaa gccaaaggtt taaatttaat
      361 aatttttgat tgttatcgac cacagagagc tgtgaatgat tttattaagt ggcttgatca
      421 atctgaacaa gtacaactta aagatcggta tcatccaaat ttatccaaat ccgaactgct
      481 tggtccttac attgcagaga aatctgaaca tactaaagga gtcactttgg atgtcagcct
      541 tacaaaacaa aatgtaaatg gtgaatacca acaaatagag atgggttcat catttgactt
      601 attcgatcct ctttcaaata ctgataatcc ttctatcagc tcgtttcata gaaataatcg
      661 ctatctatta aaggagttaa tggagggcga gggttttatt gcttacgata tggaatggtg
      721 gcattttacg tttaatcaat caaaagaatc agaattgtat tatgattttg ttatacgata
      781 atcaaagaat tctaattatt tgaatatgag atatgaacaa actaataaca atcactagga
      841 tctctatatc actgctgatt attttcatat tagctaatcc aactctggct gctcttgaga
      901 gtgataaaaa acctaatatt gtcttggtgt taatggacaa ttttggctat ggtgaaattg
      961 gtgtttatgg tgggggagtt ttacgcggtg caccaacacc caatatcgac agcattgcaa
     1021 ctgatggatt tcagctaaca aattataatg ttgaggcaga atgtactccg tcacgctcag
     1081 cattaatgac aggtagatat ggtattcgta cgcgacaacg tcaggatggt ccgcctagag
     1141 gggtatggta cggcataact caatgggaaa ttaccttggc cgaaatgctc tctaatgaag
     1201 gttatgcaac tgggatcttc ggaaaatggc atttaggaga caccaaaggt cgctatccta
     1261 cagatcaggg ctttgatgaa tggaatggaa ttcccagatc atcagatcga gccttttggc
     1321 cggatagtaa tagctttacc ccagatagtc acccagacgc tgattttgct cacgttatgt
     1381 catcatttcg aggtcaagaa ccaaaggaat tagaagttta cgaccgagcc aaaagagcga
     1441 ctatcgataa agatatcact gatcatgcca ttgatttcat caaacgcaag gcaagttcta
     1501 accaaccatt ttttgcctat ctcccctata cacaaacgca tgaaccagta gatccacatc
     1561 ctgacttttt cggcactact ggaaacggaa gctttgctga tgttttggct cagacggatg
     1621 cctatatcgg tgagctgtta gatactattg aagaactagg attgaaagaa aacaccatat
     1681 ttatttttac atcagacaat ggaagagaag gcgtgccgag atcttttggt tttactggtc
     1741 cttggcgagg aagtatgttt tctccttatg aaggatcttt acgggtacct ttcctgatcc
     1801 gctggccagg gaaaatccca aatcagcaag tatcaaatga aattttccat cagatagatg
     1861 tgtttcccac tttggctagt ttcataggag ttgatattcc gaaagatcga ataattgatg
     1921 gtgttgacca atctgatttc ctaatgggga aaactaataa gtcagctagg gactccatgg
     1981 tcatttacat cggtaatgaa ctgttcggtg cgaaatggcg taattggaaa attctgatta
     2041 aggaaatgga tgaagatgga tatggtatta agaatatggc ttatccctca gtttataatt
     2101 taatcgtaga tccaaaagag gaagtaccag agctcaacta tttaaatgac acatgggtag
     2161 attttccttt atatcaagtc ttagaagatc atgaagtatc tattgatg
//
LOCUS       DASCTH010000225         2894 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000400236, whole genome shotgun sequence.
ACCESSION   DASCTH010000225 DASCTH010000000
VERSION     DASCTH010000225.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000400236"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="QGH35_04750"
     CDS             complement(<1..773)
                     /locus_tag="QGH35_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024575.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81876.1"
                     /translation="MIDTLTWLFPDYQKLSSLVKKHDNAHGILLIGPPGIGKRIMAFE
                     LAGDFLNTDKLKIKINQENQLDIFHPDCNLICPEENKTSISIDKIRSLKSSLELTSHQ
                     GNGKVGIIYPAESMTLSAANSLLKILEEPPDETLLILITESAKKLPDTVVSRMQNHNV
                     KKPTAEETIQWLNNNQTEENWKEIIDLFGSLPLLFSELGYEFLNAKINSTIKDINALI
                     LKKSKPSEIATIWVKADLEIALKILYVLISNLITRSLIKT"
     gene            complement(766..1392)
                     /gene="tmk"
                     /locus_tag="QGH35_04755"
     CDS             complement(766..1392)
                     /gene="tmk"
                     /locus_tag="QGH35_04755"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017839578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HJL81877.1"
                     /translation="MSGFFITIEGIECVGKTTNAKFIVQYLSKYGIKTHQTREPGGTV
                     LGEKIRNMLLFEEKKNITPLIELLLLFAAREANTEQVIKPALKEGTWVVCDRFTDASI
                     AYQGFGRELGYQCVENFKEIILKNFEPDITFLLDAPVKIIRSRKKLNQNDRFESETTD
                     FFEKVRNGYLDTARRYNDRIKVIDASKPLEDVQLEIKKYLKAIINHHD"
     gene            complement(1514..1744)
                     /gene="acpP"
                     /locus_tag="QGH35_04760"
     CDS             complement(1514..1744)
                     /gene="acpP"
                     /locus_tag="QGH35_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011080818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HJL81878.1"
                     /translation="MSIEERVKKIVAEQLGSGEDEISNESSFIDDLGADSLDTVELVM
                     ALEEEFDIEIPDDDAEKIATVQAAIDFVSANS"
     gene            complement(1833..2597)
                     /locus_tag="QGH35_04765"
     CDS             complement(1833..2597)
                     /locus_tag="QGH35_04765"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006081230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HJL81879.1"
                     /translation="MTNTNKNLNLKGEISLITGATRGIGNSIAEVFARAGSIVIGTAT
                     TQEGAERITTNLSNVGLKGHGIVLDISQSGQISNQIKELKKLDLMPSILVNNAGISLE
                     NLLMRIKDEDWEKMISTNLSSAFFLCKAVIGPMLKKRHGRIINIGSIQGTTGAIGHAH
                     YSAAKAGLVGLTKSLAREVGSRGVTVNTIAPGYIITDMTKDIGDEINKSLLKQIPLER
                     FGEPIDIANAALFLASSSGSYITGETIHINGGMHMS"
     gene            complement(2620..>2894)
                     /locus_tag="QGH35_04770"
     CDS             complement(2620..>2894)
                     /locus_tag="QGH35_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81880.1"
                     /translation="FYGFGWGGLYTLIQLLSADLFGIASLGKIMGVINILDTFGGGLG
                     PFLTGYLYDRTEGYLTPFIVISLLLIIALLSSSLLSIDDQEIEDNR"
BASE COUNT          847 a          541 c          468 g         1038 t
ORIGIN      
        1 gtcttgatta atgatctggt gattaaattt gatattaaaa catagagaat ttttaatgca
       61 atttctaagt ctgcttttac ccatattgtt gcaatctctg agggtttaga tttttttagt
      121 atcaaggcat taatatcctt gatagtgcta ttgatctttg catttaaaaa ttcataacca
      181 agttcactga ataagagagg aagcgaacca aacaagtcaa taatttcttt ccaattctcc
      241 tcggtttgat tgttatttag ccattggatt gtttcctcag cagttggttt ttttacatta
      301 tgattttgca tcctactaac aacagtatca ggtagctttt ttgcagattc tgttattaaa
      361 atcaacagtg tttcatctgg aggttcttct agaatcttca ataaactatt tgcagcagac
      421 aaggtcatgg attctgcagg ataaataata cccacttttc cattaccttg gtgactggtt
      481 agttctagag aggattttaa tgatcttatt ttatcgatgg aaatacttgt tttattctct
      541 tcagggcaga tcaagttgca atctggatga aatatgtcca attgattttc ctgatttatt
      601 ttgattttaa gtttgtccgt atttagaaaa tctcctgcta attcaaatgc cattattctt
      661 tttccgattc cgggaggacc aatcagcagg atgccatggg cattgtcatg tttctttact
      721 agactggata atttttgata atccggaaaa agccatgtta gcgtatcaat catgatggtt
      781 aattattgcc tttaagtatt tttttatttc aagctggaca tcttccaaag gttttgaagc
      841 atcaattact ttgatcctat cattataacg gcgtgcagta tcaaggtagc cgtttctaac
      901 tttctcaaag aaatcagttg tctcagattc aaatcgatca ttttgattta gtttttttct
      961 gcttcgtatg atcttcacag gagcatctaa caagaaagta atatcgggtt caaaattttt
     1021 taggataatt tctttaaaat tctcaacaca ctggtaacct agttctctgc caaatccttg
     1081 ataagcaatg ctagcatcag tgaaacgatc acaaacgacc caagtccctt cttttaatgc
     1141 aggtttaatt acttgttcgg tattggcttc tcttgcagca aatagtagga gtaactcaat
     1201 taaaggagtg atattttttt tctcctcaaa taatagcatg tttcttattt tctctcctaa
     1261 gaccgtccct ccgggttctc tagtttgatg ggttttgatt ccatatttgc tgagatattg
     1321 aacaataaac tttgcattgg tggttttacc aacacactca attccttcaa ttgttataaa
     1381 aaaacccgac atatctttta tcttcttgcg gaacacttac caaggcatac caatcctatc
     1441 atacgaatgg attggttatc tgtagataaa agtgattggc tttgagagtt ttttagtcag
     1501 acaagatact ggattaactg ttagcactaa cgaaatcaat tgcagcttga acagttgcaa
     1561 ttttttctgc atcgtcgtca ggtatttcta tatcaaattc ttcttctaga gccattacaa
     1621 gctcgacagt atcaagtgaa tctgcaccta aatcatctat aaaactggac tcgttagata
     1681 tttcatcttc acctgatcca agttgttcgg caacgatttt ttttaccctt tcttcaatgc
     1741 tcataacttt cattcctcta aaattaaaaa tttggtgtat tctatgctaa tcatacattt
     1801 tcttaaacta ttttatttaa aaaaatttat agctaagaca tatgcatccc gccgttgatg
     1861 tggattgttt cacctgtgat gtaagaacct gaagatgatg ccagaaaaag agctgcattt
     1921 gctatatcaa tgggttcacc aaatctttct aatggtattt gtttcagtag agatttattt
     1981 atttcatcac caatgtcttt tgtcatatcg gtgatgatat aacccggtgc aatggtattg
     2041 actgtgaccc ctctggagcc cacttctcta gctaatgatt ttgttaaacc aacgagtcct
     2101 gctttagcag cactataatg tgcgtgacca atagcccctg ttgttccttg tattgagcca
     2161 atgtttataa tccttccatg tctctttttt agcatagggc caatgacggc tttgcacaga
     2221 aaaaacgcag aagatagatt agttgaaatc attttctccc aatcttcgtc tttaattctc
     2281 attaaaagat tttccaaact aattccagca ttattgacta gaatggatgg cattaagtct
     2341 aatttcttaa gttcttttat ttgattcgat atttgtcctg attgagaaat atctagaacg
     2401 attccgtggc cttttaggcc aacattggaa agatttgttg taattctttc cgcaccttcc
     2461 tgtgtagtgg ctgttcctat gactattgat ccagctcttg caaacacttc tgcaatacta
     2521 ttaccaattc ctctagtggc accagtaatc aaagagatct cacccttgag atttaggttt
     2581 ttattagtat ttgtcatcag taaattataa ctatatcagt taccggttat cctcaatttc
     2641 ctggtcatca atactgagaa gtgaagatga tagcaaggct atgattaata ataagctaat
     2701 gacgatgaat ggagttaaat atccttctgt gcggtcatat aagtatccag ttaagaatgg
     2761 tcctaatcca ccaccaaagg tatccaatat atttataaca cccataatct tacctaaaga
     2821 tgcaattcca aataaatctg cactgagtaa ttgaatcaac gtatataaac caccccaacc
     2881 aaaaccataa aacc
//
LOCUS       DASCTH010000226         2166 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000400537, whole genome shotgun sequence.
ACCESSION   DASCTH010000226 DASCTH010000000
VERSION     DASCTH010000226.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000400537"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            141..1304
                     /gene="metK"
                     /locus_tag="QGH35_04775"
     CDS             141..1304
                     /gene="metK"
                     /locus_tag="QGH35_04775"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HJL81881.1"
                     /translation="MSEASYLMTSESVGEGHPDKMADQISDAILDAILNEDKGCRVAV
                     ETLVKTGLVVLAGEITTSAEVDYESIARDTIKQIGYDKEELGFDHKSCKVLSAISKQS
                     PDIAMGVDRDSKENQGAGDQGLMFGYATNETTELMPTTIMLSHQLVKKQSQVRKSNKL
                     QWLRPDAKSQVTVTFNSGKPEFIDTVVLSTQHDENISMDEIRSGVMEHIIQPTLPVNL
                     VTNDTKFLINPTGSFVIGGPVGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRS
                     AAYAARYVAKNIVAAELADRCEVQVSYAIGVAKPTSVRIKTFDTEKISKEKITALVDE
                     HFDLTPFGIMNMLDLLQPIYQKTAAYGHFGREDEDFPWEKTNKAEDLKSHSSL"
     gene            1337..>2166
                     /locus_tag="QGH35_04780"
     CDS             1337..>2166
                     /locus_tag="QGH35_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010598836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HJL81882.1"
                     /translation="MVELARNRDEDFKIADMSLAEWGEKEIMIAETEMPGLMSLREEF
                     GSQKPLKGTRIAGCLHMTIQTAVLIETLIELGADIRWSSCNIFSTQDHAAAALAAKGI
                     PIFAWKDETEEEYWWCVEQTIHGPDDWTPNMLLDDGGDLTLVMHEKYPELLKNVKGVS
                     EETTTGVKRLYDMEKDSTLLCPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVA
                     GKIAIVAGYGDVGKGCAQSLSALGATVWVTEIDPICALQAAMEGYRVVTMDDACSQAD
                     "
BASE COUNT          731 a          379 c          480 g          576 t
ORIGIN      
        1 cacaaaaata taaaaattat tttctactac tttaaaaaac tagtcgcaag aattttcata
       61 gtaatcatat accttaataa acacttattg ctataatcat ttccttataa attattaata
      121 atgtaaatat tgagatacat atgagtgaag cttcatattt aatgacttca gaatcagtag
      181 gcgaaggaca tcctgataag atggctgacc aaatatcgga tgcaattttg gatgccatcc
      241 taaatgaaga caaaggatgc agagtggcag tggaaacctt agtcaaaact ggtcttgtgg
      301 ttctagctgg agaaataacc acttctgctg aagtggatta cgaatccata gcaagggata
      361 cgattaaaca aattggatac gacaaagaag aattaggttt cgatcacaaa tcttgtaaag
      421 ttttatcagc aatcagtaaa caatctccag atattgccat gggagttgat agagattcaa
      481 aagaaaatca gggtgctgga gaccaaggtc tgatgtttgg ctatgccaca aatgaaacaa
      541 ctgaattaat gccaacaact attatgctgt ctcatcaatt agttaaaaaa caatctcaag
      601 ttaggaaaag taacaaattg cagtggctca gacctgatgc aaaaagccaa gtcaccgtaa
      661 catttaatag tggtaaacct gagtttattg ataccgttgt attatcaact cagcatgatg
      721 aaaatatcag catggatgaa attagaagtg gagtgatgga gcacatcatt caacccactc
      781 tcccggtaaa tcttgttacg aatgacacaa aattcttaat caatccaacg ggtagttttg
      841 tcattggcgg ccccgttggt gattgcggtc tcactggtag aaaaattatt gtcgatacct
      901 atggcggtat ggcacgacac ggagggggag cattttctgg gaaagatccg tcaaaagtag
      961 atagatctgc agcttatgcg gcaagatatg tggcaaaaaa tatagtcgcg gcagagcttg
     1021 ccgaccgatg tgaagttcaa gtctcctacg caattggggt tgcaaaacca acttcagtga
     1081 gaattaagac ttttgatact gaaaaaatca gcaaagaaaa aattactgcg ctggttgatg
     1141 agcactttga tttgaccccc tttggcatca tgaatatgct tgatttactc caacccatat
     1201 accaaaaaac agcagcttat ggtcattttg gcagagaaga tgaagacttc ccgtgggaaa
     1261 aaactaataa agctgaagat ttaaaaagcc attctagttt ataattgttt gattaaaaat
     1321 attctgagga ctaaccatgg ttgaactagc aagaaataga gacgaagatt ttaaaatcgc
     1381 tgatatgagt ttagctgaat ggggcgagaa ggaaattatg atcgcagaga ctgaaatgcc
     1441 tggattaatg agcctcagag aagaatttgg atcacaaaaa ccactaaaag ggaccagaat
     1501 cgcaggctgc ttgcatatga ccattcaaac agctgttctt attgaaacac tgattgaatt
     1561 gggtgccgat atcagatggt cctcttgtaa tattttctca acacaagatc atgcagctgc
     1621 agcacttgca gctaaaggga ttcctatttt cgcatggaaa gatgaaacgg aagaagaata
     1681 ttggtggtgt gtggaacaaa caattcatgg accagatgac tggaccccca atatgctctt
     1741 agatgacggt ggtgatctga ctctggtgat gcatgaaaaa tatcctgaat tgcttaagaa
     1801 cgtcaaagga gtatctgaag aaacaaccac gggcgtgaaa agactctatg acatggaaaa
     1861 ggactcgact ctgctgtgtc cagcaatcaa cgtcaatgat tcggtgacaa aatcgaaatt
     1921 tgacaatctt tatggatgcc gagaatcttt ggtggatggt atcaaacgag caacggacgt
     1981 catggttgca ggtaagattg caattgttgc aggctatgga gacgtcggta aaggctgtgc
     2041 ccaaagtctc agtgcacttg gtgcaactgt ttgggtgacg gaaatagatc ctatctgtgc
     2101 cctgcaagct gctatggaag gatatcgtgt ggtaactatg gatgatgcct gttcacaagc
     2161 agatat
//
LOCUS       DASCTH010000227         3584 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000402458, whole genome shotgun sequence.
ACCESSION   DASCTH010000227 DASCTH010000000
VERSION     DASCTH010000227.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000402458"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /gene="tsaE"
                     /locus_tag="QGH35_04785"
     CDS             complement(<1..248)
                     /gene="tsaE"
                     /locus_tag="QGH35_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HJL81883.1"
                     /translation="MLESVKTLFDDTETMDYGSKLARCFSDSPADSSVIYLKGDLGAG
                     KTTFAKGFLRGMGYEGNVTSPTYTLIELYRTEVYDIYHV"
     gene            complement(233..1720)
                     /locus_tag="QGH35_04790"
     CDS             complement(233..1720)
                     /locus_tag="QGH35_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013425.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HJL81884.1"
                     /translation="MTISDNYVYPSTEVSKIDNLAIKQEGIPGFVLMRRAADFSYQSA
                     IECYPNTNSFIVFCGLGNNAGDGYLFASRALDEGKKAQVVYLSTPENLKGDAYLAYQE
                     YEKLGGQIFPWSKDRELCADLCVDAIFGVGLNRSVDGVFLQAIQTINQSPIPVISLDI
                     PSGLNPDTGEKMEDCISAQMTTTFVGYKKGLFLNSGPDCSGKISYSDLDIPECCFDSA
                     KPSLRIVDKELSKNLLKKRPKTSHKGSFGHILIVGGNHGMGGAVRIASEAALRSGAGL
                     VSVVTRAANIPIVTKLRPEIMCHSVDEDPNKINDFIAKASVVAIGPGLGLDDWAMTLF
                     EQVFQSNLPMVVDADALNILSLKPRKRKNWILTPHPGEAARLIGITNPRVQSDRIGSL
                     QKLADSFSGTIVLKGNNTLIGSSNEMPYLITVGNPGMASAGMGDLLTGIIAGLLGQFK
                     DIDQGLVSAVAAYIHGSSGDLAAEQGERGLIATDLFRHLQQCLNP"
     gene            1770..2834
                     /gene="queG"
                     /locus_tag="QGH35_04795"
     CDS             1770..2834
                     /gene="queG"
                     /locus_tag="QGH35_04795"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HJL81885.1"
                     /translation="MAQNPNAIKTLIHQWSKELGFDGFGVAGIEIQKDEKHLQSWLDR
                     KFHGSMNYMEKHGLKRSRPDVLLPGTIRVISLKMHYFSREKDQALALLKKNEVGYIAN
                     YAVSNDYHQIIKERLKVIIAKIKDYSELQGQNYFVDSAPVLERALARNAGLGWIGKNT
                     NLIHNSSGSWFFLAEIFTDIPLPIDRSSINHCGTCSACIDICPTQAFTMPYQLDARKC
                     ISYLTIENKKSIAVELRPAIGNRIFGCDDCQIICPFNKFAKKIEISDLLPKEELLSQP
                     LEQLFLWSEKEWDDTTQSTALARAGYDGWLRNIAVALGNARTTERIVDALRSRNNQQS
                     QMVNEHIYWALEQHQKKSNQ"
     gene            complement(2859..>3584)
                     /locus_tag="QGH35_04800"
     CDS             complement(2859..>3584)
                     /locus_tag="QGH35_04800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007144718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HJL81886.1"
                     /translation="KGASRYYKSIGNYFVTIKIMGNVVPLKLESRLVVIEGPIGVGKT
                     SLAKRLSDHVSGKLILEEPEKNPFLENFYLDQESNALPTELSFLFQRAKHIEGINQDD
                     LFSDTIIADFLFDKGSIFSELNLNTEELNLYQQVKNSLQINPPTPDLVIYLQAPVDVL
                     ISRLHVRQTSVDHLIESSYLERLADSYAKFFYNYDDSPLLVVNAESIDPVHNDDHFLL
                     LYEEAVNVKYGKHFFNSTAAAFT"
BASE COUNT         1154 a          708 c          656 g         1066 t
ORIGIN      
        1 acatgatata tgtcataaac ttcagttcga taaagctcaa tcaaagtata tgtgggactg
       61 gtcacatttc cttcatagcc catgcctctg agaaatccct tggcaaaagt agtttttcct
      121 gcacccagat cacctttgag ataaataaca gaagaatctg caggtgagtc agaaaaacat
      181 ctggctagtt tagagccata atccattgtc tctgtatcgt caaataaggt ctttacggat
      241 tcaagcattg ttgtaaatgt ctaaatagat cagtagctat cagtcctctt tccccctgtt
      301 ctgcagcaag atcaccagaa gatccatgga tgtaggcggc cactgccgat accaatcctt
      361 ggtcaatatc tttaaattgc ccgagtagac cagcaatgat ccctgtcagc agatcaccca
      421 tccctgctga agccatcccc ggattgccca ctgtgatcag ataaggcatc tcatttgagg
      481 atcctatcaa tgtattgttg cctttcagta caattgttcc agaaaaacta tctgctagtt
      541 tttgaagtga acctattctg tctgattgca ccctaggatt agttatacct attaatcttg
      601 cagcttcacc tggatggggt gttaaaatcc agtttttcct ctttctgggc ttaagagata
      661 aaatattgag ggcatcagca tccacaacca tgggcaaatt tgactgaaat acttgttcga
      721 ataatgtcat tgcccaatca tccaatccca atcctgggcc aattgcaaca acggaggctt
      781 tagcgataaa atcatttatt ttgttgggat cttcatccac tgagtggcac atgatttcag
      841 gtcttaattt agtaacaatt ggaatattag cagctcttgt aaccacgctt accaatcctg
      901 ctccagagcg aagagccgcc tctgatgcaa ttctgactgc tccacccatg ccatgattgc
      961 cgccaacaat taagatgtga ccaaagctgc ctttatgaga tgttttgggt ctttttttca
     1021 aaagattttt tgataattct ttatcaacaa ttcttaacga gggttttgca gaatcaaagc
     1081 aacactcagg gatatctaaa tcagaataag aaattttgcc agagcagtcc ggtccagaat
     1141 ttaaaaagag acctttttta taaccaacaa aggtagttgt catttgtgca gaaatacagt
     1201 cttccatttt ctcaccagta tcaggattta gaccagaagg aatatccaat gaaataaccg
     1261 gaattggtga ttgattaata gtttgtattg cttgtagaaa aacaccatca actgatcgat
     1321 tcagaccaac accaaaaatt gcatcaacac atagatcagc acataattct ctatctttac
     1381 tccatgggaa aatctgccct cctaatttct catattcctg atatgcaaga taagcgtctc
     1441 ctttcagatt ctcaggagtt gataaataaa ccacctgagc tttcttccct tcatccaagg
     1501 cccgtgacgc aaataaatac ccatcccctg cattatttcc tagaccacaa aaaacaatga
     1561 acgagtttgt gtttggataa cattctatag ctgattggta agaaaaatca gcggcacgtc
     1621 tcattaaaac aaatcctgga ataccttctt gtttgatagc cagattatca atctttgaga
     1681 cttccgtaga tggatataca taattatctg aaatcgtcat actaatcatt atactttgct
     1741 taaaaataaa actctctcag agaaagaaaa tggctcaaaa tcccaatgct ataaaaaccc
     1801 tcattcacca atggtctaaa gagcttggat ttgatggttt tggtgttgcc ggcatagaga
     1861 tacaaaaaga tgagaaacac ctccaatcgt ggttggatag aaaattccat ggatcgatga
     1921 actatatgga aaaacatgga ctaaaacgat ctcgaccaga tgttttgctg cctggaacga
     1981 ttagggtcat ttcattgaaa atgcattatt tctcaaggga aaaggatcag gctcttgcac
     2041 tactaaaaaa aaatgaggtt ggctacattg caaattacgc agtaagcaat gactatcatc
     2101 aaataattaa agaaagactt aaagtaataa ttgcaaaaat taaagattac tcagaattac
     2161 aggggcagaa ttattttgtt gactctgctc ctgttttgga aagagctttg gctagaaatg
     2221 caggactagg ttggattggt aagaatacca atttaatcca taactctagt gggtcttggt
     2281 tttttctggc agaaattttt actgatattc cgcttcccat tgatcgatct tcaataaatc
     2341 actgtggcac ttgctcggct tgtattgata tttgtccgac tcaagctttt accatgcctt
     2401 accagctcga tgcaagaaag tgtatttctt acttaacgat agaaaataag aaatccatag
     2461 cggttgaatt aagaccagca attggtaata gaatctttgg ctgtgatgat tgtcagatta
     2521 tctgcccctt taataagttt gccaagaaaa ttgaaataag tgatctatta cccaaagaag
     2581 agctattaag ccagccctta gaacaacttt ttctgtggtc tgaaaaagaa tgggatgata
     2641 ctacccaaag cacggcatta gctagagctg gttatgatgg ttggctcaga aatattgcgg
     2701 tggccttggg caatgctaga acaacggaaa gaattgttga tgcactgaga tcgagaaaca
     2761 atcagcaatc tcaaatggtt aatgaacaca tctattgggc actggaacaa caccagaaaa
     2821 aatctaacca ataaaataat attgatcgga caaagagatt aagtgaaggc agctgcagta
     2881 gagttaaaga agtgcttccc gtacttcaca ttaaccgctt cttcatacaa taaaagaaag
     2941 tgatcatcat tgtgtacggg atcaatactc tctgcattaa ctaccaataa tggtgaatca
     3001 tcataattgt agaaaaattt ggcataagaa tctgcgagcc tctccagata gcttgattca
     3061 attaaatggt cgactgatgt ttgtctgacg tgaagtctag aaattaatac gtctacaggt
     3121 gcctgtaaat agatcactaa gtctggagtc ggtggattga tttgaagaga gttttttact
     3181 tgttgatata aattcagttc ctctgtattc aggttcagtt ctgaaaatat ggaaccctta
     3241 tcaaaaagga aatcagcaat gatggtatct gaaaataaat catcttgatt gataccctct
     3301 atgtgtttag ctctctgaaa taaaaaagac agttcagtgg gtaatgcatt tgattcttga
     3361 tcaagataaa aattctctaa aaaaggattc ttttctggct cttccagaat taatttcccg
     3421 gaaacatgat cggataagcg cttcgcaaga cttgttttgc caacaccaat cgggccttcg
     3481 attacaacaa gtcgagattc taacttgagt ggtacaacat tacccataat ctttatagta
     3541 acaaaataat tacctatact tttataatac ctactagctc cctt
//
LOCUS       DASCTH010000228         1854 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000403828, whole genome shotgun sequence.
ACCESSION   DASCTH010000228 DASCTH010000000
VERSION     DASCTH010000228.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000403828"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="QGH35_04805"
     CDS             <1..606
                     /locus_tag="QGH35_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81887.1"
                     /translation="GMVARGGFSAWLAQNFVKLVVWWHGPVTFEELKAASQETLNTEN
                     PSPSQGAAMFSKWIAHSYYAEDMDIVGIIENFGKIDLTDEEVRAYEAPYPTGEYKAGP
                     HVWPYLIPEQLQENEKYWVNIYEKWDKPFLVAFGGEERITLRMKDDFINRVPNPTVIT
                     LKGASHFVQEEVGPELASVINDFIADRPVRGFSKQGDSLDK"
     gene            672..1409
                     /locus_tag="QGH35_04810"
     CDS             672..1409
                     /locus_tag="QGH35_04810"
                     /inference="COORDINATES: protein motif:HMM:NF036707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81888.1"
                     /translation="MKLKKPIIYSLLALSVVISVFGLQPFQQTIDAEKALVKQTGVYA
                     TEVRRLPDASYLVAVRTSMPEVKVDMVRWWFSDFLQTTEHYNWWHPKDHVWMDWENKE
                     PGEIIGASHLVHEYIGGNLSKLRIQFVNSAEFFGYDANDEDTFVICARVGLLDEEMNI
                     AKMCHVVRNTLNGAEMRSRFWLGHVAKRKGNKTISSLEGLIGNTFLTRLLVLNKQSAI
                     DLKHHAEEEMKYLAALLPPLYGSQNID"
     gene            1485..>1854
                     /locus_tag="QGH35_04815"
     CDS             1485..>1854
                     /locus_tag="QGH35_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJL81889.1"
                     /translation="MDHQDEYYDNMITMLELIWGEGYMAPGGLGNIEKMFDGIETAGK
                     RILDIGCGLGGPAFEMANTFGADVVGIDLEAPLIKRATETAIKMGLQDRCKFRIVKEG
                     PFDFPDQSFDIVASSGAITQI"
BASE COUNT          611 a          290 c          411 g          542 t
ORIGIN      
        1 gggatggtag ccagaggtgg tttttctgct tggcttgccc agaactttgt gaagttagtg
       61 gtatggtggc atggtcctgt tacttttgaa gagctaaaag cagcatcgca agaaacatta
      121 aatactgaaa atccttctcc aagtcaaggc gcagctatgt tttcaaaatg gatagctcat
      181 tcatattatg cagaggatat ggatatcgtc ggaatcattg aaaattttgg aaaaatcgat
      241 cttacagacg aagaagtaag agcttatgaa gctccttatc caactggaga gtacaaagca
      301 ggaccgcatg tatggccata tctcatacca gaacaacttc aagagaacga gaaatattgg
      361 gttaatattt atgaaaaatg ggataaacct tttttagtgg cattcggagg tgaagaaaga
      421 attactctta gaatgaaaga tgactttatt aacagagtac ctaacccaac agtaataact
      481 ttaaaaggag ctagtcattt tgttcaggaa gaagtgggac ctgaacttgc ttctgtgatt
      541 aatgatttta ttgctgatag accggtaaga ggattcagta agcaagggga cagtttagat
      601 aaataaaagt attttccttg aaaaataatt tataaaacaa atcccaggtg agtaactatt
      661 gagtaggaag gatgaagctt aaaaagccta taatatatag tttactagca ttatcagtcg
      721 ttatatctgt ctttggtttg cagccgttcc aacaaaccat agatgccgag aaagctttag
      781 ttaaacaaac aggagtttat gctaccgaag ttcgcagatt acctgatgct tcttatctag
      841 ttgccgtaag aacttcaatg cctgaggtca aggtagacat ggtgcgttgg tggttctctg
      901 attttcttca aacaacagaa cattacaatt ggtggcatcc taaagatcat gtatggatgg
      961 attgggaaaa taaagaacca ggagaaatta taggtgctag ccatttagtg catgaatata
     1021 tcggagggaa tttgtccaag cttagaatac agtttgttaa ttctgctgag ttcttcggtt
     1081 atgatgctaa tgatgaagat acatttgtta tttgtgcaag agtaggtttg cttgatgaag
     1141 aaatgaatat agcaaaaatg tgtcatgtcg ttagaaatac actaaatggg gcagaaatga
     1201 gatcccgatt ttggttagga catgttgcta agcgtaaagg aaacaaaacc atctcctcat
     1261 tagagggctt gatcggtaat acttttttaa cgcgtctact tgttcttaat aaacagagtg
     1321 caatagattt aaaacaccat gcagaggaag aaatgaaata tttggcagca ttactcccac
     1381 ctctatatgg atcacaaaat attgattaag ataaaaaaat agttacacat attatttaat
     1441 tcccctggaa ccctttattt ataattatta caacggagaa cagaatggat caccaagacg
     1501 aatattacga caatatgatt actatgttgg agctgatttg gggagaaggc tacatggcac
     1561 caggcggttt gggcaatatt gaaaaaatgt ttgatggcat tgaaacagca ggaaaacgta
     1621 tccttgatat tggttgtggt ttaggaggcc cagcttttga gatggccaac acatttggtg
     1681 cagatgttgt tggcatcgac cttgaggcac cactaatcaa gagagccact gagacagcaa
     1741 ttaagatggg attacaggat agatgcaagt ttcgcattgt caaagaggga ccatttgatt
     1801 ttcctgatca gtcttttgat attgttgcct catcaggtgc gatcactcaa atat
//
LOCUS       DASCTH010000229         3776 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000416772, whole genome shotgun sequence.
ACCESSION   DASCTH010000229 DASCTH010000000
VERSION     DASCTH010000229.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000416772"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="QGH35_04820"
     CDS             <1..204
                     /locus_tag="QGH35_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020481506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HJL81890.1"
                     /translation="PEFRNEFITDNGNIILDVHNLSIIDPPELEKKLNNIPGIVSNGI
                     FASNAANVILVGKNDEVLTFNNQ"
     gene            201..1109
                     /locus_tag="QGH35_04825"
     CDS             201..1109
                     /locus_tag="QGH35_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJL81891.1"
                     /translation="MKQTRRSLLKLIGASTLGSSLKVDVLKAQERMIQRIIPSSGGLL
                     PVIGLGTSRVFDTDFSPELLKPRKEIVQALLNIGGRLIDSSPMYGRAEELTGKIVKDL
                     NIGEQLFLATKVWIEGKEAGEIQMNESSIKLNKAVIDLMQIHNLLDWKTHIKTLYQWK
                     EEGKISYIGITHSRSSAFEQMENIIRKESIDFAQFNYSLGETEAENRLLPLCKDLGVA
                     TIINRPFMRGKLFKAAKKKALPNWCHEFNINSWSQFFLKFILANDAVTNIIPATSKPK
                     HMIDNLGGGIGPVPSKELQKRMLDSF"
     gene            complement(1112..1501)
                     /gene="rpsI"
                     /locus_tag="QGH35_04830"
                     /pseudo
     CDS             complement(1112..1501)
                     /gene="rpsI"
                     /locus_tag="QGH35_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019625663.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
     gene            complement(1695..2123)
                     /gene="rplM"
                     /locus_tag="QGH35_04835"
     CDS             complement(1695..2123)
                     /gene="rplM"
                     /locus_tag="QGH35_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012639337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HJL81892.1"
                     /translation="MKTFSAKHDEVERHWYLVDATNKTLGRLSTEIASRLRGKHKPIY
                     TPHVDTGDYIVVVNASKIRVTGNKMTDKVYYKHSGYIGNMKSMNLETMLEKNPERVIM
                     KSVKGMLPKNKLGNAMLKKLRVFAGPEHNHSSQKPEVLEL"
     gene            complement(2206..2874)
                     /gene="rpe"
                     /locus_tag="QGH35_04840"
     CDS             complement(2206..2874)
                     /gene="rpe"
                     /locus_tag="QGH35_04840"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011768594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HJL81893.1"
                     /translation="MADYQIAPSILSADFSCLGEEVEMVLDAGADIIHFDVMDNHYVP
                     NLTVGPVVCKALRNFGIEAPIDVHLMVSPVDDLVVSFAEAGATYISFHPEASENIDAT
                     IQLIKKHDCKPGLAINPDTSLDCLDDRLGSIDLVVIMSVYPGFAGQSFIQSSLQKLEG
                     VRESIIASGKPILLEIDGGIKANNIRDVAQHGADVFVSGSGIFGTENYRKTIEHMRAE
                     LSKV"
     gene            2992..>3776
                     /locus_tag="QGH35_04845"
     CDS             2992..>3776
                     /locus_tag="QGH35_04845"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HJL81894.1"
                     /translation="MKTPIINNLELIAQGKVRDIYAIDEDHLLIVTTDRMSAFDVVLP
                     DPIPGKGEILTQLSHFWFKQTENIVQNHLSDDFTIEEILEDHTNIDYYTTRSMVVKRL
                     KPLPIEAVVRGYLIGSGWKDYQNSGSVCGIALEDNLQMAAQFSEPIYTPATKAEFGEH
                     DENISYEKTVDILGEDLASKVKDHAVAIYNYGVQHASKRGIIIADTKFEFGLDEDGAL
                     YLIDEVLTPDSSRFWAMEDYHLGISPPSFDKQYLRDYLETLDW"
BASE COUNT         1166 a          752 c          713 g         1145 t
ORIGIN      
        1 ccagaattca gaaatgaatt tattacagat aacggcaaca tcatacttga tgttcataat
       61 ctaagcatca tagatccacc tgagcttgag aaaaaactca ataatattcc tggtattgtt
      121 agcaatggta tatttgcaag caatgcagca aatgtaattc tggtcgggaa aaatgatgaa
      181 gtactgacct tcaataatca atgaagcaaa ctagaagaag cctgttaaaa ctgatcggtg
      241 cctcgactct aggttcttca ctgaaagtag atgtgctcaa agcacaagaa agaatgattc
      301 aaagaataat cccatcctct ggtggattgt tacctgttat tggcttaggc acttcaaggg
      361 tttttgatac agatttctca ccagaactct taaaacctag aaaagagata gtgcaagctc
      421 tactgaatat tgggggccgt ttaattgatt cttcaccaat gtatggaaga gcagaagaat
      481 tgactggaaa aattgtcaaa gatctaaata ttggagaaca gttattttta gctaccaaag
      541 tttggattga aggcaaagaa gcaggtgaaa tacaaatgaa tgaatcttcc ataaaattga
      601 ataaagctgt catcgacctc atgcaaatac acaacctgct tgattggaag acgcatataa
      661 aaacactgta tcagtggaaa gaagaaggga aaattagtta cattggcata actcattcca
      721 gatccagtgc gtttgagcaa atggaaaaca tcattagaaa agaaagcata gattttgccc
      781 aattcaatta ttcactgggt gagacggaag cagaaaatag actgttgcca ttatgcaaag
      841 acctaggtgt agctaccata attaatcgac ctttcatgag aggtaaatta tttaaagcag
      901 ccaaaaaaaa ggccctccca aattggtgcc atgaatttaa tatcaatagt tggtcacaat
      961 tcttcttgaa atttatttta gccaacgatg ccgtcaccaa cataatcccg gcaacatcaa
     1021 aaccaaaaca tatgatcgat aatcttggtg gtggaattgg cccagtgcca agtaaggaat
     1081 tacaaaaaag gatgctggat tcgttctaga attaacgttt cgagtattgt gttgcttttc
     1141 ttgccttttt taggccaact tttttccgct caacctttcg tgcatctcgg gttaagaaac
     1201 cttttcctct tagtggtgcc cttagatttt catcgtactg aagaagtgcc ctactcaatc
     1261 catgtctgat tgctccggct tgcccactgc cgccgccgcc tcttacggtg gcattaacat
     1321 ctactttatc aagcaattca gttgtttcca gaggctgttt aactatcatt cttgcagttt
     1381 ctcgatcgaa aaattgatct aaaggtaact tattcaccac aatgcttcct ttgccctttt
     1441 ttagataaac tcgggcagta gatgtttttc ttctcccagt accataaaaa acttcactcg
     1501 ccttagctgc cttaggtttg gctttggttt cagctgcagg ctcttctgcc ggtgtttctt
     1561 tagctgtctt aggtttggct ttggtttcaa ctgcaggctc ttctgccggt gtttctttag
     1621 ctgtcttagg ttcggctttg gtttcagctg caagctcttc tgccggtgtt tctttagctg
     1681 ccataaaaac tcccttataa ctccaacact tctggtttct gtgagctatg attgtgctca
     1741 ggaccagcaa aaactcttaa tttcttcaac atggcattac ccagcttatt tttcggtagc
     1801 atgcctttaa ccgatttcat aataactctc tcaggatttt tctccagcat ggtttcaaga
     1861 ttcatagatt tcatatttcc aatgtaacca ctatgcttat aataaacctt atccgtcatt
     1921 ttatttccgg ttaccctaat tttagaagca ttcactacta cgatgtaatc accagtatcc
     1981 acatggggtg tataaattgg cttatgctta cctcgcaaac gactggcaat ctctgtggac
     2041 aatcgtccca aagttttatt agtagcatcg actaagtacc aatgcctttc tacctcatcg
     2101 tgctttgctg aaaatgtttt catgctaatt cttattcatt atgaaggacg ctagtctact
     2161 catctcagct atatttttca atgaaatctc tgtaaataaa gaagtttaga cttttgataa
     2221 ctcggctctc atgtgttcaa tggtttttct ataattttct gtaccaaaaa tacctgaacc
     2281 agaaacaaaa acatcggcac catgttgagc cacatccctt atattattag cttttatacc
     2341 tccatctatt tccaacaata ttggtttccc agatgcgata atggactctc tgacaccttc
     2401 gagtttttgt aaagagctct gaataaaact ttggcctgca aatccgggat aaacagacat
     2461 aataacaaca agatcaatgg acccaaggcg atcatcgaga cagtctagtg aggtgtcagg
     2521 attaatagcc aacccaggtt tacaatcatg ttttttaatg agctgaatgg ttgcatcaat
     2581 attttctgat gcctcaggat gaaaactgat ataggtcgca ccggcctcgg caaaactaac
     2641 tactaaatca tccaccggac tcaccattaa atgaacatca ataggagctt caatgccaaa
     2701 atttctcagt gccttgcaaa caacagggcc gacagtgagg ttgggaacat aatgattatc
     2761 cataacatca aaatggataa tgtcagctcc tgcatcaaga accatttcaa cttcttctcc
     2821 caggcaagaa aagtctgctg acagtataga cggtgcaatt tgataatcag ccatggctca
     2881 atagctctct tttaatcaat aaaaaataca atactatcat gccatgatgt gagtgatcat
     2941 agagaaattt actttattga taaatgggcg agtaattctt aaaatacata gatgaaaact
     3001 ccaataatta ataatttaga acttattgca caaggcaaag ttagggatat ttatgcgatt
     3061 gatgaagatc atttgctgat tgtgacaacg gatcgaatgt ctgcatttga tgtcgtctta
     3121 cctgatccaa ttccaggtaa aggtgaaatc ctgacacaac tttctcattt ttggtttaag
     3181 caaactgaaa atatcgtgca aaatcacctt agtgatgatt tcacaataga agaaatccta
     3241 gaggatcata ctaatattga ttattacacg acaagaagca tggtggtcaa gcgtctaaaa
     3301 ccattgccca tagaggctgt tgtcagaggt tacttgattg gatcaggttg gaaggattat
     3361 cagaacagtg gttcggtgtg tggtattgct ttagaagata atctgcaaat ggctgctcaa
     3421 ttttccgaac caatctacac tccagcgacc aaagcagagt ttggtgaaca cgatgagaat
     3481 atttcgtatg aaaaaaccgt cgatattcta ggtgaggatc tagctagcaa agtaaaggac
     3541 catgcagttg ccatatacaa ctatggtgtc cagcatgcca gcaaacgtgg aatcattatt
     3601 gccgatacaa aatttgagtt tggtttggat gaggatggag cgttatatct aattgacgaa
     3661 gtgctgactc cagattcatc aagattctgg gcaatggaag attatcatct tggtatcagt
     3721 ccacccagtt ttgacaagca gtacctgaga gactatttag aaacattgga ttggga
//
LOCUS       DASCTH010000230         3720 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000418496, whole genome shotgun sequence.
ACCESSION   DASCTH010000230 DASCTH010000000
VERSION     DASCTH010000230.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000418496"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="QGH35_04850"
     CDS             <1..191
                     /locus_tag="QGH35_04850"
                     /inference="COORDINATES: protein motif:HMM:NF019640.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81895.1"
                     /translation="LSEAAEILFAATGGKGLYLDNHLQRGFRDIKGGVNHISSSWDIA
                     GPLWGAHAFGLEPKIPMY"
     gene            complement(192..2105)
                     /locus_tag="QGH35_04855"
     CDS             complement(192..2105)
                     /locus_tag="QGH35_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014795.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJL81896.1"
                     /translation="MRSLFKQFFDNDISRRDFGTKLLALGFSQVAVNTFLSAAVQARE
                     TLPDEGKRIEGTGAEILAETLRAAEVEYVFGTSSTGMSPFYDALTLKNDIKFISAIAE
                     SQATSMAHGYELVTGKTAVLFIPGVAIPSAMNNLYNAWKDRSSIAVLSDGSSSNFAGR
                     NGFQQLDDWLDPMTVFTKWRWQVDNDRQISEMTRRTIKLAETPPGGPVYLRFPGSILK
                     KPKIKQKIYPQSQFRIPMGLHPKPELIEATAKALLEAQKPLMILGHEVTRAKANKEAV
                     ELADLLGIRMAQGYSVYGDVPFKHPLFCGFYGLGIPRGLSKTDVFLNLGAPMPDPTIF
                     TPSIPSKAKAIHARIEFDDIANSHPTDIAIAAGMKETVIAIKESVQSLATMNRIKVLR
                     EDRMQASIEINAKFEARKEQQAKATWNSSPLSWARASFEIDRNLDDHAIVVAELDTRE
                     PFDWMNLGPEQKWLIGGSTGFALGWGIGASLGAKIAEPNRQVACLVGDGAMLFGQIES
                     LWSASRYDIPVIIIVCNNMSYDFERNRIIQASPLAAKKETRDMWRDISCYLGNPEVEF
                     VGLANSFDIQGKQANTPEELIQCLQQAKDVTVSGRPFLIDLRLMQLDLRGFETQQTWH
                     PDISIAAKRTKKV"
     gene            complement(2113..2517)
                     /locus_tag="QGH35_04860"
     CDS             complement(2113..2517)
                     /locus_tag="QGH35_04860"
                     /inference="COORDINATES: protein motif:HMM:NF016001.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HJL81897.1"
                     /translation="MKAMTIWHRMLFKGYGASSLGTFKGNPICMITTVGRKSGNPVSI
                     PLLTIPHQDDYILVASQGGAPTHPAWYYNIVHEPRIKMTIKSKTYDMFAKQLSDEEKE
                     DLWPVIVNACSVYDDYRQRTSRNIPVFLCSKQ"
     gene            2658..3065
                     /gene="fur"
                     /locus_tag="QGH35_04865"
     CDS             2658..3065
                     /gene="fur"
                     /locus_tag="QGH35_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HJL81898.1"
                     /translation="MMDKNKEIRSAGLKVTDARRSILRLLETHPHSHLSAYDVHQQLF
                     EKKKSVGIATVYRVLNQFVEAGICVRHNFETGHSLYELAPDDHHDHMVCLLTGTVIEF
                     EDKLIEQRQQQMAAEKGYKIIDHNLILFVEPID"
     gene            complement(3073..>3720)
                     /locus_tag="QGH35_04870"
     CDS             complement(3073..>3720)
                     /locus_tag="QGH35_04870"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HJL81899.1"
                     /translation="LDKIKEKNVDAVSQGTENLKKHIENIHLFGLPVVVAINQFTHDT
                     EQEISMIKEKCDYLGVDVVICNHWRDGGKGAMDLAKAVVDLTENHKTDFNFLYPDDMS
                     LWNKMKTIATSVYGANNIMADKDVRTEIKRLEEQGFGQLPVCMAKTPASLSTDPKLKG
                     RPTGFDVPIREVRLCSGAGFIVSLTGDVITMPGLPKTPAAENIDVDDKGLIQGLF"
BASE COUNT         1057 a          802 c          697 g         1164 t
ORIGIN      
        1 tgttaagtga ggctgctgaa attctctttg ctgccactgg gggtaaagga ttgtatctgg
       61 ataatcatct ccaaagagga tttagagata ttaaaggagg agtcaatcat attagttcaa
      121 gttgggacat tgctggtccc ttgtggggag cccatgcgtt tggtttagag ccaaagattc
      181 ctatgtatta atcaaacttt ttttgttctc ttggctgcga ttgaaatatc tggatgccat
      241 gtttgctgag tttcaaaacc ccttaaatct aactgcatca atctcagatc aatcaaaaaa
      301 ggtctaccac tcaccgtcac gtcttttgct tgctgtaaac attgaatcaa ttcttccggt
      361 gtatttgctt gctttccctg gatatcaaaa ctgttggcta aaccaacaaa ttcaacctct
      421 ggattaccca aataacaact gatatcccgc cacatgtctc gagtttcctt cttagctgct
      481 aaaggcgatg cttgaattat tctatttctc tcgaaatcat aactcatatt attacaaacg
      541 ataatgatga cgggtatgtc ataccttgat gccgaccaca acgattcaat ctgtccaaac
      601 agcatcgcgc catcaccaac taagcacgca acctgacgat tgggttcggc aatttttgca
      661 cccaaagagg caccgatacc ccaacccaat gcaaaaccgg ttgagccacc aatgagccat
      721 ttttgttcag gaccaagatt catccaatcg aaaggttctc tggtatccaa ctcagcaaca
      781 acgatcgcat gatcatcaag atttctgtct atctcaaagg atgcccttgc ccaagaaaga
      841 gggctagaat tccaagttgc ttttgcttgt tgctcctttc tggcttcaaa tttagcattg
      901 atctcaatgc ttgcttgcat cctgtcttct cttaagactt tgattcgatt cattgttgct
      961 agggattgta ctgactcttt gatcgcaatc accgtttctt tcataccggc agcaatagca
     1021 atgtcggttg gatggctgtt ggcaatatca tcgaattcta tccttgcatg gatagcttta
     1081 gccttgcttg ggatggatgg tgtaaatatt gttggatccg gcatgggggc tcccaagttg
     1141 agaaaaacat cggttttact caaacctctc ggtataccca atccatagaa tccacaaaac
     1201 aaagggtgct taaacgggac atcaccatag acactatagc cttgagccat tctgatacct
     1261 aatagatctg caagttcaac cgcctcttta ttggcttttg ctctggtgac ttcgtgccct
     1321 agaatcatta ggggcttttg tgcttccaaa agagcctttg cggtggcttc tattaactca
     1381 ggttttggat gcaatcccat tgggattcta aactgactct gcggatagat cttttgttta
     1441 atctttggtt ttttaaggat agatcctgga aaacgtaagt aaacaggacc gccgggtgga
     1501 gtttcagcaa gtttgatcgt tctcctagtc atttcactga tttgacgatc attatccacc
     1561 tgccatcgcc atttagtaaa aactgtcata ggatccaacc aatcatcaag ttgttggaac
     1621 ccatttcggc ctgcaaagtt agagcttgaa ccgtcagata aaactgcaat cgatgatcga
     1681 tctttccaag cattgtagag attattcatg gcactcggga tggctacacc tggaataaaa
     1741 agaacagcgg tcttgcctgt taccaactca taaccatgag ccattgatgt tgcctgagat
     1801 tcagcgattg ctgagataaa ctttatatca ttttttaaag tcaaagcatc ataaaatggt
     1861 gacatgcctg tgctgctggt accaaaaaca tattccacct cagcagctct aagggtctca
     1921 gcaagaatct ctgcacccgt accttcaatt cttttccctt catcaggtaa ggtctcgcga
     1981 gcctgtactg ctgcagataa aaaggtgttc accgcgactt gactgaatcc taaagcgagc
     2041 agttttgttc caaaatctcg tctagatata tcgttatcaa aaaattgctt aaacaatgat
     2101 ctcataaatg actcactgtt tgctgcaaag aaagacgggt atatttctac tcgttcgttg
     2161 gcgataatca tcataaacag agcaagcatt cacaatgact ggccaaagat cttccttttc
     2221 ttcatcggat aattgtttag caaacatgtc gtatgttttt gatttaatgg tcatctttat
     2281 tctgggttca tgaacaatat tgtaatacca agccggatga gtaggtgctc ctccctgtga
     2341 ggcaactaat atatagtcgt cttgatgtgg gatggttagc aaaggaatgg atactgggtt
     2401 acctgatttc ctgccaacgg tagtaatcat gcaaatggga ttacccttaa aagtgccaag
     2461 actactagca ccataacctt taaaaagcat tctgtgccag attgtcatag ctttcatgat
     2521 cggttttacg agtctgtaag tattcattct aaagataata caacaaagat aagggtttca
     2581 ttgtatatga atgtgaaaac cttatgaact ttactaagct acttgctatt ctaattattc
     2641 gttatatgat tcttgggatc atggataaaa ataaagaaat tagatctgca ggactgaaag
     2701 ttactgatgc caggagaagc atattaaggc tcctagaaac tcatccacac tctcatctaa
     2761 gtgcctacga tgttcatcaa caactttttg aaaaaaagaa aagtgttggt attgctactg
     2821 tttacagagt tttgaatcaa tttgttgaag caggtatttg tgtgcgtcac aactttgaaa
     2881 ccggtcattc cttatatgaa ttagccccag acgaccatca cgaccacatg gtttgcctat
     2941 tgacaggaac ggttattgaa tttgaagata aattaatcga gcaaagacaa caacaaatgg
     3001 ctgctgaaaa aggctataaa attattgatc acaacctcat tttgttcgtt gaaccgatcg
     3061 attagccaaa atctaaaata acccttggat taatccttta tcatccacat ctatattttc
     3121 cgcagccggt gtttttggta accctggcat tgtaatcaca tcgccggtta gtgacactat
     3181 aaagcctgct ccagagcata atctgacttc tctaatcgga acatcgaacc cagtcggcct
     3241 gccttttagt tttggatctg ttgataaaga ggctggtgtt ttagccatgc acacgggtaa
     3301 ttgtccaaac ccttgttctt ctagtcgttt tatttctgtt cttacgtctt tgtcagccat
     3361 aatattattg gcaccataca cagatgtggc aatggttttc attttattcc aaagactcat
     3421 atcatctggg tatagaaaat taaaatctgt tttatgattc tctgttaaat ccaccactgc
     3481 ttttgcaaga tccattgcac ctttccctcc atctctccag tgattgcaaa taaccacatc
     3541 aacccctaaa taatcacatt tctctttaat cattgaaatc tcttgttctg tatcgtgggt
     3601 aaactgattg atagcaacaa ccactggcaa accgaacaga tgaatattct caatgtgttt
     3661 ttttaagttc tcggtaccct gacttacagc atcaacattt ttttctttaa tcttatcaag
//
LOCUS       DASCTH010000231         2373 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000418878, whole genome shotgun sequence.
ACCESSION   DASCTH010000231 DASCTH010000000
VERSION     DASCTH010000231.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000418878"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="QGH35_04875"
     CDS             complement(<1..859)
                     /locus_tag="QGH35_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HJL81900.1"
                     /translation="MNDTQKTEPLPRACLILPTYNEVGNIEPLIKEIFTVQSNIDTHH
                     LHVIVIDDHSPDGTADIVEQQMKTYSRLHLISGPKTGLGDAYKRGFSFALETFNPDLL
                     LQMDADGQHDPKHIPQFIDLTNKGYDLVIGSRLVMGGGTPHFTIWRRFLSVFGNLLVR
                     FAGGINSVKDCTSGFRCIKADLVTKCNLGVFSTRGYSFQSSLVYELLRNDAKPIEIPI
                     MFDARTKGRSKLSFSDQVEFVFNLTKIAYRNSGEFIKYCFVGILGVMVNIGVYTFLVK
                     YFQVRIEIAP"
     gene            complement(906..2306)
                     /locus_tag="QGH35_04880"
     CDS             complement(906..2306)
                     /locus_tag="QGH35_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014425.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HJL81901.1"
                     /translation="MISSRLKNIYILSLILVISFTTRFLYLGEIPGPVFDEVFYPIYG
                     YSYLTDQQFFYAHPPLGNYILAATIWIYCQLPWVEISPLNTLSFEALPVLSYRWLNAL
                     SGSLLCILSYILSQKLFKNKNFSLLVCFFVAIDGSLLVDSRVGLINIYLVFFGLCALI
                     CAANAKFVDLQNRHWLLLCGVFIGLAASIKWNGLGYLLTILSFMAFVIAVRTTDALRL
                     NHSKKAVEQLHDLVGQNNTFSWEYVLYFLIIPLAVYSLIWIPDLRFNTEYDFLEKHQQ
                     LLGYHQIMVTEDEHPYCSKWYSWPFMIRPISYYFNSYEVIGDLGNKMTMFQDIHLFPN
                     PPLYWFSTIAILLMGFEWLKLLWHWIREKTVSNKFFFFTFILCGYCANLLPWAWISRC
                     AFLYSYQPASIFGFFALAWYLAHLMSAESIKYRILSWGMLILIIAAFFYWLPTLTGMP
                     LEAESWLQRKWFESWI"
BASE COUNT          843 a          451 c          427 g          652 t
ORIGIN      
        1 gtggagcaat ttcaatgcga acttggaaat acttcactaa aaaggtatag acacctatat
       61 tcaccatcac tcctaaaatg ccaacaaaac aatattttat gaattctcca gaatttctat
      121 aggcaatttt tgtaagatta aacacgaatt caacctggtc gctaaaagag agcttggacc
      181 gaccttttgt tctggcatca aacataatag gaatttcaat aggtttggcg tcatttctaa
      241 gcagctcata aaccaaagaa gattggaacg aataccctct cgtagaaaac actcccagat
      301 tgcatttagt aacaagatct gccttaatac accgaaaacc cgaagtacag tctttaacac
      361 tattaattcc tcctgcgaaa cgaactagca ggtttccaaa cacacttaga aaccttcgcc
      421 aaatagtgaa atgaggtgtg ccgcctccca taacaagcct tgatccaatg accagatcat
      481 aacccttatt agtaaggtca ataaattgcg gtatgtgttt tggatcgtgt tgaccatcag
      541 catccatttg caatagtaga tcaggattaa atgtttctaa tgcaaaagaa aaacctcttt
      601 tataagcatc gcccaaacca gttttgggac cggaaattaa gtgaagacga gagtatgttt
      661 tcatttgttg ctccacgata tctgcagtgc catccggcga atggtcatca ataacaatta
      721 catgcaggtg atgtgtatca atattcgatt ggacagtaaa aatttctttt atgagcggtt
      781 caatatttcc aacttcgttg tatgttggta gaataaggca cgctcgtgga agcggttctg
      841 ttttttgagt atcattcatg acgttgtcgc taattcagga taactatttc ggatcatcat
      901 gaccactaaa tccatgattc aaaccatttc ctttgaagcc atgattcagc ttctaaaggc
      961 attccagtta gggtagggag ccaatagaag aaagcagcaa taattaggat caacattccc
     1021 caagaaagta ttctatattt aatcgattct gcagacatca aatgagccaa gtaccaagca
     1081 agagcaaaga atccaaaaat ggaagctggc tggtagctat agagaaaagc gcaccgagag
     1141 atccaagccc aaggtaaaag attggcacaa taaccacata atataaaggt gaaaaagaaa
     1201 aatttatttg ataccgtttt ttccctaatc cagtgccaga gtaatttcaa ccattcaaag
     1261 cccatcaaca gaattgcaat tgttgaaaac caatacaaag gtggatttgg gaataaatgg
     1321 atgtcttgaa acatggtcat cttattcccc aaatctccaa taacctcata actattaaaa
     1381 tagtaactaa tgggccttat catgaatggc caagagtacc atttggaaca ataaggatgt
     1441 tcatcttctg tcaccatgat ttggtggtag ccaagtagtt gttggtgttt ctctagaaaa
     1501 tcatattctg tattaaaacg gagatcagga atccagatta atgaatagac agccaaagga
     1561 ataattaaaa aatacagtac atattcccaa ctaaaggtat tgttttgtcc cactaaatca
     1621 tgcaactgtt ctactgcttt ttttgaatga ttaagtctga gagcgtcggt ggttcgaacc
     1681 gcaataacaa acgccataaa agacagaata gtgagtaaat agcccaaacc attccatttg
     1741 attgatgcag cgagacctat gaaaacacca cataacagca accaatgtct gttttgtagg
     1801 tcgacaaatt tagcgtttgc ggcacatatc aaagcgcata atccaaaaaa aaccaaatag
     1861 atattgatta acccaacacg tgagtcaact agcagcgatc catcgatggc aacaaaaaaa
     1921 caaaccaaga gtgaaaaatt tttattttta aataacttct gtgaaagaat gtaagacaaa
     1981 atacacaata atgatcccga tagagcattt agccatcgat agcttaggac aggtaatgct
     2041 tcaaacgata aagtgttcag tggactaatc tcaacccaag gtaattgaca atatatccag
     2101 atagttgctg caagaatata gttacccaga ggagggtgtg cgtagaaaaa ctgctgatct
     2161 gttaaataac tatacccata tattggataa aaaacttcat caaatacagg tcccggaatc
     2221 tcacctaaat acaaaaatcg tgttgtaaac gaaatgacaa ggatcaatga gagaatgtaa
     2281 atatttttta accgactgga aatcattata atttttctat atagcctata tagatagcag
     2341 ctggttgaaa agtttttatt gacgcttggt tat
//
LOCUS       DASCTH010000232         3813 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000422366, whole genome shotgun sequence.
ACCESSION   DASCTH010000232 DASCTH010000000
VERSION     DASCTH010000232.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000422366"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /gene="nagZ"
                     /locus_tag="QGH35_04885"
     CDS             complement(<1..740)
                     /gene="nagZ"
                     /locus_tag="QGH35_04885"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HJL81902.1"
                     /translation="MRLGPLFISISGTELSRDEEQILLNDLVGGVVLFSRNYQNKDQL
                     KMLVNSIKALKYPSLFVSVDQEGGRVQRFQDDFYPIPEMRSLGKMYDQSDHIAMQAAY
                     YAAKILGIELMNVGIDFSFAPVLDIDYGVSEIIGSRSFHSDPKIVGHLASSFFDGFQD
                     IGMQCIAKHFPGHGFVKEDSHQNTPVDQRSIRTISKDLQPYKNLINKGLRGIMTAHIK
                     YPLVDKKLATFSKFWLIEYLQKELQFNG"
     gene            complement(755..1228)
                     /locus_tag="QGH35_04890"
     CDS             complement(755..1228)
                     /locus_tag="QGH35_04890"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012570000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HJL81903.1"
                     /translation="MTTRIKISICSQTLELIDSEGVETYLVSTSKYGPGEVKNSFCTP
                     RGQHIIRAKIGDGQKIGTIFKERRSTGEVFSKKYHNLQEGDWILTRILWLSGIEIGKN
                     RLGDVDTMQRFVYIHGVPDQNILGIPGSKGCINMSNNDVKDVFDAVVAGTPVDIE"
     gene            complement(1239..1967)
                     /locus_tag="QGH35_04895"
     CDS             complement(1239..1967)
                     /locus_tag="QGH35_04895"
                     /inference="COORDINATES: protein motif:HMM:NF021613.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HJL81904.1"
                     /translation="MNYLIFDVETIPDLDYGRRFLNLDGLNNEDIGRAMFFQQQQKTG
                     EEFLPLYLQKVISISVLIDAEDTLELKSLVVNGDDEKLIIQSFYNTLEKEDYCLVTWN
                     GLLFDLPVLNYRALFNQVDAASYWQKKSWHIDIKDSLSGHHPKEQGSLDQVSRGLGFP
                     GNINLRGGQVWNSYLEGNEEGISNDCDFDVFNTYLVFLKYQTMIGKLSEDQLSIKLKQ
                     FRIMLKDTNKKHFEVFLKRWEKQS"
     gene            complement(1969..2355)
                     /gene="acpS"
                     /locus_tag="QGH35_04900"
     CDS             complement(1969..2355)
                     /gene="acpS"
                     /locus_tag="QGH35_04900"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HJL81905.1"
                     /translation="MMGIFGIGIDLVNVKRIQKLNEKYGQRFTDKILCSEEKELFYSQ
                     SNPVKFLANRFAGKEATVKALGTGFSQGITWKDIGVIQHKTGKPQLIFSEKTQSLLQK
                     HHITNSHISLTDEGHWAQAFVVLEKN"
     gene            complement(2352..3089)
                     /gene="pdxJ"
                     /locus_tag="QGH35_04905"
     CDS             complement(2352..3089)
                     /gene="pdxJ"
                     /locus_tag="QGH35_04905"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HJL81906.1"
                     /translation="MTRKQKLLLGVNVDHIATLRQARGTTYPDPVEMALIAEEHGADS
                     ITVHLREDRRHIQDRDVERLVRLTRTRVNLEMALSREMIDFACSIKPSDCCLVPEKRE
                     ELTTEGGLNVMDQIDPLSQASAKLKKSGIRVAFFVDPDPRQIESSQLGGAEVVELHTG
                     NYADAVNDNDQEYELNRIIECAIHGTRIGLKVHAGHGLNYENTAPIASIEEILELNIG
                     HAIVAKSVDLGMAKAVSSLKKIMMDAR"
     gene            3200..>3813
                     /locus_tag="QGH35_04910"
     CDS             3200..>3813
                     /locus_tag="QGH35_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012398.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJL81907.1"
                     /translation="MTKLSQFATVDPFDGMNSKNPGKAQNLINGEWVDTPKYFDDIPD
                     PITGEYFIDIPDTEDLSDFIQNLEKCPKSGLHNPLKNNDRYIMLGNVCAKAAALLADP
                     EVEGFFVRLIQRVMPKSDNQCLGEVRVTRTFLENFAGDGVRFLGRSFSNPGDHLGQES
                     SGYRWPFGSVVIVAPFNFPLEIPALQFLGALFMGNRPLIKSATTV"
BASE COUNT         1147 a          759 c          629 g         1278 t
ORIGIN      
        1 atcccattaa attggagttc tttttgcaaa tattctatta gccaaaactt agaaaatgtt
       61 gccagtttct tatctacgag tgggtacttg atatgtgcag tcatgatgcc tcttagacct
      121 ttatttatta gatttttata gggctgtagg tctttgctaa ttgtccgaat tgacctttga
      181 tcaacaggtg tattttgatg cgaatcttct tttacaaaac catgaccggg gaaatgtttt
      241 gcaatgcact gcattccaat atcttgaaag ccatcaaaaa acgaggaagc caaatggcct
      301 actattttgg gatcactgtg gaaggatcga ctgccaataa tttctgaaac tccataatct
      361 atatctaaaa caggggcaaa actaaaatct ataccgacat tcatgagctc aattcctaga
      421 atttttgctg cataatatgc tgcttgcata gcaatgtgat ccgattgatc atacattttt
      481 cctaaagatc tcatctcagg aattggatag aaatcatctt gaaatctttg cactctgcca
      541 ccttcctgat caacacttac aaacaaacta ggatatttta gcgccttgat ggagttgaca
      601 agcattttca actgatcctt gttttggtaa tttctggaaa ataaaacaac accgccaaca
      661 agatcattca gcaagatttg ttcttcatct ctggaaagct ctgtgccgct aatggagata
      721 aataatggac ctaatcgcat tgcaatatta attattattc aatatcaact ggagtaccag
      781 caacaacggc atcaaaaaca tctttgacat cattattgct catgttgatg catcctttag
      841 aacctggaat tcctaaaata ttttgatctg gtactccgtg gatgtagaca aaacgctgca
      901 tggtatcgac atcaccgagt ctattttttc caatttctat gccactcaac caaagaatcc
      961 ttgttaaaat ccaatcaccc tcttgtaaat tgtgatactt tttagaaaat acttcgcctg
     1021 ttgatcgtcg ttctttaaag attgtgccta ttttttgacc atctccaatt tttgctctga
     1081 taatgtgctg acctcttggg gtgcagaagc tatttttcac ttcaccaggt ccatattttg
     1141 aagtagaaac taagtatgtt tctacacctt cagagtcaat taactcaagt gtttgggaac
     1201 aaatagaaat tttaatcctt gtagtcacaa aaacaaaact agctttgttt ctcccatctt
     1261 ttaaggaata cttcaaaatg tttcttattg gtgtctttga gcattattct gaattgtttt
     1321 aatttaatgg aaagctgatc ttcagacaat ttgccaatca ttgtttgata cttaagaaaa
     1381 actagatatg tattgaaaac atcaaaatca caatcattgc taataccctc ctcattacct
     1441 tcaagatagg aattccaaac ctgccctccc cgtaaattga tgtttcctgg aaaaccaagc
     1501 cctctggaaa cttgatctag gctgccctgt tcttttggat gatgaccaga taaagagtct
     1561 ttaatatcta tgtgccaaga ttttttttgc caataagatg ctgcatccac ctgattaaat
     1621 aaagctcgat aattcagtac aggtaaatca aataaaagac cattccaagt tactaagcaa
     1681 taatcttcct tttcaagtgt gttataaaaa ctttgaataa ttaatttttc atcgtcccca
     1741 ttaacaacta atgatttaag ttccaatgta tcttcagcat cgattaacac tgaaattgaa
     1801 attacttttt gaagatagag aggcaagaat tcctctcctg ttttctgttg ctgctgaaaa
     1861 aacatggcac gaccaatatc ttcattattt aatccatcaa gattaagaaa tcgccgacca
     1921 taatcaagat ctggtatggt ttcgacgtca aatatcaagt agttcatttc aatttttctc
     1981 taatacaaca aacgcttgag cccaatgacc ttcatctgtc agactaatgt gtgaattagt
     2041 aatatgatgt ttctgaagta gtgattgagt tttctcagaa aatattaatt ggggtttccc
     2101 tgttttatgt tgaattacac cgatatcttt ccaagtgata ccttgtgaaa accccgttcc
     2161 aagggctttt actgtcgctt ccttgcctgc aaacctattt gccaagaatt ttactggatt
     2221 ggactgagaa taaaaaagct ctttttcttc agagcataaa attttgtcgg taaatctttg
     2281 accatatttc tcgttgagct tctgtattcg ttttacgttt accaaatcaa tgccaatacc
     2341 gaatatgccc atcatctggc atccatcata atttttttga ggctactaac ggcctttgcc
     2401 attcctaaat caactgattt tgcgacaata gcatgtccga tattaagctc tagaatctct
     2461 tctattgacg ctataggtgc tgtattttcg taattcaatc catgaccagc gtgcactttt
     2521 aagcctatcc tagttccatg tatagcacac tctataattc gatttaattc atattcttgg
     2581 tcattatcat taactgcatc agcataattt cctgtgtgca attcaaccac ttctgcaccc
     2641 cctaattgac ttgattcaat ttgtcttgga tcaggatcaa caaaaaaagc tactcgaata
     2701 cccgattttt ttagttttgc cgaagcttgt gataatggat ctatctgatc cattacattc
     2761 aagcctcctt cagttgttaa ttcttctctt ttctccggaa ctaaacaaca atcagatggt
     2821 tttattgaac aagcaaaatc gatcatttct ctggataatg ccatttctaa atttacccta
     2881 gttctagtta aacgaaccaa tctttccacg tcacgatcct gaatatgccg cctatcttct
     2941 cttaagtgaa ctgtgatgct atcagcaccg tgttcttctg cgataagagc catctcaacc
     3001 gggtctggat acgtggtacc tcttgcttga cgtaacgttg cgatgtggtc aacgtttact
     3061 ccaagtaata atttttgttt tcttgtcata attaatatcc aatcattctt catatgatat
     3121 aacaacatcc aattcgctga aacttgaagc ggaaatgaat ttagaataaa gtagcttgac
     3181 ttattaactg ggggaaacaa tgactaaact ttctcaattt gcaaccgttg atcctttcga
     3241 tggaatgaat agtaaaaatc caggaaaagc tcagaattta attaatggtg aatgggtaga
     3301 tacacctaag tattttgatg atattccaga tcctattaca ggagaatatt ttatagatat
     3361 cccagataca gaggacctat ctgacttcat tcaaaatctt gaaaaatgtc ctaaaagcgg
     3421 cctgcacaat ccattaaaaa ataatgatcg ttatatcatg ttgggaaatg tttgtgcaaa
     3481 agctgctgca cttcttgctg atccagaggt ggaaggcttc tttgtacgtc tgatacaacg
     3541 agttatgcca aaaagtgaca atcaatgctt gggtgaagtg agagttacaa gaactttttt
     3601 ggaaaacttt gcaggtgacg gtgttcgttt cttgggaaga agtttttcaa atccaggaga
     3661 ccacctgggt caagaatctt caggttatag atggccattt ggatctgttg taatcgttgc
     3721 tccttttaac tttccacttg aaattcctgc attgcaattc ttgggtgcac tgttcatggg
     3781 aaatcgaccg ttaatcaaat ctgcaacaac ggt
//
LOCUS       DASCTH010000233         2210 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000424638, whole genome shotgun sequence.
ACCESSION   DASCTH010000233 DASCTH010000000
VERSION     DASCTH010000233.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000424638"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="QGH35_04915"
     CDS             <1..445
                     /locus_tag="QGH35_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cytidylyltransferase"
                     /protein_id="HJL81908.1"
                     /translation="PVTFEISIFNVEKNLLSYSEIEKTLDQFRLEQNWVLTNAPMFDE
                     KALIFKQSTFILGMDTLLRLFDPKFYGNDQAMQSRLQALIANDIRFMVFGRQIGSHFM
                     TLNDFLIPEAFKNRFTGISETEFREDISSSEIKIERLREMNDKAT"
     gene            complement(480..767)
                     /locus_tag="QGH35_04920"
     CDS             complement(480..767)
                     /locus_tag="QGH35_04920"
                     /inference="COORDINATES: protein motif:HMM:NF018717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HJL81909.1"
                     /translation="MPAYFVVRCTYGDMDHYHRYEKLAGLAITEFNGRFLITGKGETV
                     QKESGKHKKTVVVEFDSMETALTCYHSQTYQQALKEIALSAERDFVIVEGL"
     gene            complement(782..1273)
                     /locus_tag="QGH35_04925"
     CDS             complement(782..1273)
                     /locus_tag="QGH35_04925"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HJL81910.1"
                     /translation="MTQEESIYKYQCRNNSGEMQSLSGFSGQVLLIVNTASKCGLTPQ
                     YQGLETLHQTYKDQGFSVLGFPCNQFGNQEPGTDEEIQTFCSTNFQVTFPVFAKVEVN
                     GMGADPLYQFLTNKKPGLMGSKKIKWNFTKFLINKNGQPIERFSPTTTPEKITEKIEN
                     ALS"
     gene            complement(1270..>2210)
                     /locus_tag="QGH35_04930"
     CDS             complement(1270..>2210)
                     /locus_tag="QGH35_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HJL81911.1"
                     /translation="KKQVPIMTNSESLLQRGNNAYGKPATALAILRETILGRELFDFA
                     FREYATRWQFKRPTPYDFFRTMEEASGVDLDWFWKGWFFTTDHVDIAINKIYEASLDT
                     LNPEVDLARDREEKNQEPPTLADLRNTEEGIITRVEERPELLDIYDEYDEFTAGDKEL
                     KQYERSMKGLTDPNASDPDWKSKTLEEALSKNENYYILEFSNLGGLVMPIPLEINFED
                     GSKELLQIPVEIWRKEPSKTKWLKQTPKKITSVVLDPYWEIADTNIENNYYPRRMIPA
                     RLKPNLDKRYEGGKNLMSDLLKRNKEITSHQQENQQ"
BASE COUNT          609 a          446 c          413 g          742 t
ORIGIN      
        1 ccctgttacc tttgagattt caatttttaa tgttgagaaa aatttattga gctactctga
       61 aattgagaaa acattggatc agttcagatt ggagcagaac tgggtattaa ctaacgcacc
      121 aatgtttgat gaaaaagcac taatctttaa gcaatcaacc ttcatactag gtatggatac
      181 attattacgc ctcttcgatc caaaattcta tggcaacgat caggctatgc aatcaagact
      241 gcaagcattg attgcaaacg acatacgatt catggttttt ggtagacaaa taggatcaca
      301 cttcatgacc ctcaatgatt tcctaattcc tgaagcattc aagaaccgct tcactggtat
      361 ttcagagaca gaattcagag aagatatcag ctcatcagag atcaaaatag agagattgag
      421 agaaatgaat gataaggcaa cctgattaat ctgacccgtt aaaagacctg attctaactc
      481 tataaacctt ctactatgac aaagtctcgc tccgccgata aggcaatttc tttaagcgct
      541 tgttgatatg tttgactgtg ataacaagtt aaagctgttt ccatggaatc aaattccacc
      601 acaactgttt tcttgtgttt tcctgattct ttttgaactg tttcaccttt gcctgtaatt
      661 aaaaatctac cattaaattc tgtaatggcc agaccagcaa gtttttcata tcgatgataa
      721 tgatccatgt caccgtaagt acatcgaaca acaaaatagg caggcattga cttatagaaa
      781 attaggacag ggcgttctca attttttctg tgattttttc aggtgttgtt gtgggcgaga
      841 atctttcgat aggctgaccg tttttattga tgaggaactt ggtaaaattc catttaatct
      901 ttttactgcc cattaaaccc ggttttttat tagttaggaa ttggtacaaa gggtctgcac
      961 ccattccatt cacctcaact ttagcaaata caggaaaggt tacttgaaaa ttggtagaac
     1021 aaaatgtctg aatttcttca tcagttcccg gttcttggtt gccaaattga ttgcagggga
     1081 atcccagtac agaaaaaccc tgatccttgt aagtttgatg cagggtttct agtccctggt
     1141 attgtggggt taatccacat ttacttgctg tgttgacaat caataatacc tgcccagaaa
     1201 atcctgagag tgattgcatc tcaccactat tgttcctgca ctggtattta taaatacttt
     1261 cttcttgagt cattgctgat tctcctgttg gtgagatgta atttctttgt ttcttttgag
     1321 cagatcactc atcagattct ttcctccttc gtacctttta tctaagtttg gcttgagcct
     1381 ggcagggatc atacgtcttg gatagtagtt attttcaata ttggtatcag caatttccca
     1441 gtatggatct aaaacaacgg aggtgatctt tttgggtgtt tgttttagcc atttggtttt
     1501 actaggttct ttgcgccaga tctctactgg tatttgcagc aactctttac tgccatcttc
     1561 aaaattaatt tctagtggaa tgggcatgac caacccaccc aaattgctaa actcaagaat
     1621 gtagtagttc tcatttttcg ataaagcctc ttccagagtc ttactcttcc aatcagggtc
     1681 actggcattg gggtctgtca aacctttcat gcttctttcg tattgtttta gttctttatc
     1741 gccagctgtg aattcatcgt attcatcata aatatccagc aactcaggtc tttcctctac
     1801 tctggtgata atgccttcct cagtatttct taaatctgcc agggttggtg gttcttggtt
     1861 tttttcttcg cgatctctag ctagatcaac ctcaggattc aatgtatcca agcttgcttc
     1921 ataaatctta ttgatcgcaa tgtcaacatg atcagtggtg aagaaccagc ctttccaaaa
     1981 ccaatcaaga tcgactcctg atgcctcttc catggttctg aagaaatcat aaggagtagg
     2041 acgtttgaat tgccatcggg tagcatattc gcgaaaagca aaatcaaaca actcccttcc
     2101 taaaatggtt tctctgagaa tggcaagagc ggttgctggt ttcccatagg cattatttcc
     2161 acgctgcaat agcgattctg aattggtcat gatgggtact tgtttcttgt
//
LOCUS       DASCTH010000234         2151 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000443562, whole genome shotgun sequence.
ACCESSION   DASCTH010000234 DASCTH010000000
VERSION     DASCTH010000234.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000443562"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="QGH35_04935"
     CDS             complement(<1..581)
                     /locus_tag="QGH35_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HJL81912.1"
                     /translation="MVRTRFAPSPTGYLHIGGVRTALFCWLYAKNQKGQFLLRIEDTD
                     VDRSTQESIDAIHEGMHWLQLDHDEEPVYQSQRMDLYQQKAEELLESGCAYRCYCTQE
                     ELDAMRKAQSEKGETPGYDGRCREIKEPKNGEFVIRFKSPEEGEIIINDHVFGNVLVN
                     NQELDDFILVRSNGMPTYHFSVVVDDHDTNISHV"
     gene            complement(594..1631)
                     /locus_tag="QGH35_04940"
     CDS             complement(594..1631)
                     /locus_tag="QGH35_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019474.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJL81913.1"
                     /translation="MINGMQSALVKFLFRLPNPILKLLTRGKTITVGGRTMTPGTQIL
                     LHILEKRRPTKDLSNADPKKLRVLYDKMAGSLDRKAAPLKIKDHELDVDQGTIPIREY
                     IPDHIEDRGNALIYFHGGGMVLCSIKTHDILCRHLANQLGWKVFSIGYRLAPEHQYPV
                     PLNDCESAFDWIADNADDFNIDSGKLAVGGDSAGGNLAASLCVRRKEESKSMPNKQLL
                     IYPATDWAGSYQSRKDYAEGFFLTLDLMKWFTNHYVVDDKDSKNIYVSPIRYNNPAGL
                     PDAVVVTAGFDPLRDEGIAYAKHLRDAGVKVIEREHEGTIHAFANFTIEPLAYRAVVQ
                     MAQDLKTITEQ"
     gene            complement(1632..1913)
                     /locus_tag="QGH35_04945"
     CDS             complement(1632..1913)
                     /locus_tag="QGH35_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019623663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HJL81914.1"
                     /translation="MAGLKETICFIVHGRVQGVFFRDSTRKVARGLGIKGSAVNRSDG
                     SVQVIAKGTPEALEELKAWLRQGPEMALVENVEEVDIENQLEFNDFIIG"
     gene            complement(1925..>2151)
                     /locus_tag="QGH35_04950"
     CDS             complement(1925..>2151)
                     /locus_tag="QGH35_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81915.1"
                     /translation="SMFNYPSEKRLPLIEHPVLVINDNSSLTEATIQANEMIKNSVYE
                     ELTDAQGGVFELNVDQIIKHVCTFLQSQCN"
BASE COUNT          586 a          497 c          397 g          671 t
ORIGIN      
        1 acatgactga tattagtatc atgatcatcg acaaccacac taaaatggta ggtaggcatg
       61 ccattggagc gcaccaaaat gaaatcatcc agttcctgat tattaactaa aacatttcca
      121 aaaacatgat cattaattat gatttcaccc tcttctggac tcttgaatcg aattacaaac
      181 tcaccatttt ttggctcctt gatctccctg catcgaccgt cataaccggg agtttctccc
      241 ttttctgact gtgcttttcg cattgcatct aattcttctt gggtgcaata gcagcgatat
      301 gcacaacctg actctagaag ctcttctgct ttttgttgat aaagatccat tcgctgtgat
      361 tgatagaccg gctcttcatc gtgatcaagc tggagccagt gcatgccttc atgaatcgca
      421 tcaattgact cctgggtaga tcgatcaaca tcagtgtctt caattcttag aagaaattgc
      481 cctttttggt tttttgcata caaccaacaa aacagagcag ttctgacccc acctatatga
      541 agatagcctg tagggctagg tgcgaaacga gttctaacca aaacactctc ccatcattgt
      601 tcagttatag tctttagatc ctgagccatt tgcacgaccg ctctataagc caaaggttca
      661 atcgtaaaat tggcaaacgc atggattgtg ccttcatgtt ctcgttctat aaccttaacc
      721 cctgcgtccc ttaaatgttt agcgtaagct ataccctcat cacgcaaagg atcaaaaccg
      781 gctgtgacca caaccgcatc tggtagacct gcaggattat tgtatcgaat aggcgataca
      841 tagatgtttt tgctatcttt gtcatccact acgtaatgat tggtaaacca tttcattaaa
      901 tcgagtgtca ggaaaaatcc ctcggcataa tctttccttg attggtaact acctgcccag
      961 tcagttgctg gataaattaa taattgcttg ttgggcattg atttactttc ttcttttctt
     1021 ctaacacaaa gacttgctgc tagattacct cctgcgctgt ctccacccac ggccagttta
     1081 cctgagtcta tattaaaatc atcggcatta tcagcaatcc aatcaaaggc gctttcacag
     1141 tcattgagtg gaacgggata ttgatgctcg ggagccaaac gatagcctat tgaaaaaact
     1201 ttccagccca gttgattggc caagtgacga cagagtatat cgtgtgtttt aatactacag
     1261 agcaccatgc caccaccatg aaagtagatc aacgcattgc cacggtcttc aatatgatca
     1321 gggatatact ctctaatggg tatagtgccc tgatcaacat caagttcgtg gtcttttatc
     1381 ttgagggggg ctgcttttct atctaaactc ccggccattt tatcatatag aactctcagc
     1441 ttttttgggt cagcatttga taaatctttg gtgggtcttc ttttctccaa gatatgaaga
     1501 agaatctgtg tgcccggagt catagttcga cccccgacag tgattgtttt ccctcttgta
     1561 agtaacttta aaatagggtt gggaagtcgg aagagaaatt ttaccagtgc tgattgcata
     1621 ccattaatca tttaaccaat tataaagtca ttaaactcca gttgattttc aatatcaacc
     1681 tcctccacat tctccaccag ggccatttcg ggtccttgcc ttaaccacgc ctttaattct
     1741 tctaatgctt ccggagtgcc ttttgcaatg acttggacgc tgccatctga tcggttcact
     1801 gcagaacctt tgatacctag acctcttgcg acctttctgg tgctatctct aaaaaaaaca
     1861 ccctggactc ttccatgaac aataaagcaa atggtttctt ttaatcctgc catcaattaa
     1921 caagttaatt gcattgagat tggagaaaag tgcaaacgtg cttaataatc tgatcgacat
     1981 tgagctcaaa gactccccct tgggcatctg ttagttcttc atagacactg tttttgatca
     2041 tttcatttgc ttgtattgtt gcttccgtca aagaactgtt gtcgttgatc acaagaacgg
     2101 gatgttcaat taatggcaat cgcttctcgg agggataatt aaacatagaa t
//
LOCUS       DASCTH010000235         4238 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000449543, whole genome shotgun sequence.
ACCESSION   DASCTH010000235 DASCTH010000000
VERSION     DASCTH010000235.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000449543"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="QGH35_04955"
     CDS             complement(<1..446)
                     /locus_tag="QGH35_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012993.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HJL81916.1"
                     /translation="MNSTDSVSENLDILDPLLLKASKQGACLVLLPENFAFMGSHKQL
                     VSNIMEDFNDGPIQSFVAEKSKEYGIWIIAGSIPIKNDDQSRSYARSMVFDSNGALIS
                     FYDKIHLFDVVVNEKIYKESSFIEPGSTPVSIQTPWFKLGLSICYDV"
     gene            complement(478..1653)
                     /locus_tag="QGH35_04960"
     CDS             complement(478..1653)
                     /locus_tag="QGH35_04960"
                     /inference="COORDINATES: protein motif:HMM:NF024893.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HJL81917.1"
                     /translation="MTRIIKICLVTIVMLFLTVVATSSILLPKYISSWKNPVIARMNL
                     VFDAVIDYQDLDFKWLTINDFSINPILTFDQFSMELFQQEDKGLQANIVEIEIDFLAS
                     FKSRSTRIKSLLIDGSEILITHSPENGFMFDQIPIKKLIVLSYPGARRPLLTLKNVKA
                     RSVDIETLQEQFGLQSFIASNNSEFDLDMIWSEIPRRKQIIHGEGSMKINIDDGQITL
                     LERVPGKIFSLFSLSKLPKRLLLDFSDITKKGFSFKKVTADFQFNNGSAYTCDLVIKG
                     TTADIVIIGATDLIGRTFNQLAIVQPAISDMLPMGGAMLGGPAVAASVFIFTKLLRKP
                     LKEVGVNYYSINGPWENPKIELIPTNEVALSFFDDCQDYLAETIESQDALNNSVEIN"
     gene            complement(1735..2205)
                     /gene="rlmH"
                     /locus_tag="QGH35_04965"
     CDS             complement(1735..2205)
                     /gene="rlmH"
                     /locus_tag="QGH35_04965"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HJL81918.1"
                     /translation="MKIKMHAIASKSIAWLDDGFSVYQKRLPPHIQLHLNKIPLAQRK
                     KNQPIDHALNQEGKALLSLIKKDETLILLDEDGEMLSTKEFSLLIEKWMLQSTKPVMA
                     IGGPDGFSDLVHQRADRCLSLSRLTLPHAFVPAILAEQIYRAWTILEGQPYHRN"
     gene            complement(2202..2546)
                     /gene="rsfS"
                     /locus_tag="QGH35_04970"
     CDS             complement(2202..2546)
                     /gene="rsfS"
                     /locus_tag="QGH35_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020483035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HJL81919.1"
                     /translation="MHQELLKLTLTVLNDMKAKAVSSIDVSSITSISDYMIFATGNSS
                     RHVRSIADKVAESVKKEGHAVLGVEGYETSQWVLIDLGDIIVHIMQEETRSFYKLEDL
                     WSVGDQTVRPQL"
     gene            complement(2551..3564)
                     /gene="holA"
                     /locus_tag="QGH35_04975"
     CDS             complement(2551..3564)
                     /gene="holA"
                     /locus_tag="QGH35_04975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HJL81920.1"
                     /translation="MKLSSSELINHLSEKVHSIYFLSGSEDLLIIESLDELRKAAALN
                     DYIDKATFTVTGRFKWGAVDDSLKSQSLFGGKKYIEIQIPNSRPGKQGSEAIVKLIEN
                     LYEDTLLVFVAGKLEKDIKQAKWVKELQKKAISIECPKVYPRQFRGWLTNRLTRHGLG
                     IDRDALEMFVLLTEGNLIVAKQSIERLLMMEVTGKVTMENISQCVADGAHFDLFQLTE
                     AAFLKNPTRVYRIYERLKSEGMRPEQILAVLYWEIKNLMDASLDIDGGLSVKQAMQKA
                     RIWPSKQKIIEPYLISQSKTQIGQLVDQACKVDNVIKGVVIGNPWEEIGYLLFMFSTP
                     ETI"
     gene            complement(3561..3818)
                     /locus_tag="QGH35_04980"
     CDS             complement(3561..3818)
                     /locus_tag="QGH35_04980"
                     /inference="COORDINATES: protein motif:HMM:NF015899.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HJL81921.1"
                     /translation="MDPKLLDIICCPVFREKLSFVTSKELQVINTAIGNKTLKKLDGS
                     VAKDTVNNALINSSKTLLYPIEDDIPLLLDDAAIELKEIDI"
     gene            complement(3822..4076)
                     /gene="yidD"
                     /locus_tag="QGH35_04985"
     CDS             complement(3822..4076)
                     /gene="yidD"
                     /locus_tag="QGH35_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HJL81922.1"
                     /translation="MIAPKQLAKSVIIAYQKLLSPFFKPSCRFQPTCSQYALEALDRF
                     GLMKGMTLSLTRLFRCNPLFNSGYDPVPETFRFFSSPKNS"
     gene            complement(4088..4219)
                     /locus_tag="QGH35_04990"
     CDS             complement(4088..4219)
                     /locus_tag="QGH35_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014688.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81923.1"
                     /translation="MSMIGLDEHLSPTRNNGLNIMLERIKNDANNMVASQKIKTKVN"
BASE COUNT         1318 a          836 c          710 g         1374 t
ORIGIN      
        1 acatcatagc aaatagaaag acccagtttg aaccagggcg tttgaatact tacaggtgtg
       61 gaaccgggtt caataaaact tgattctttg taaatttttt cattgacgac cacatcaaaa
      121 agatgaattt tgtcataaaa agaaatcaag gctccatttg agtcaaaaac catagatcgg
      181 gcataacttc tgctctgatc atcgttctta attgggatag accctgcaat aatccatatt
      241 ccatattctt tcgatttctc agcaacaaag gattgtattg gaccatcatt aaaatcttcc
      301 ataatgttac taactaactg tttatgagag cccataaaag caaagttctc cggcagtaaa
      361 accaaacaag cgccctgttt ggaagccttt aggagtaagg ggtctagaat atccaaattt
      421 tcagatacgg aatctgtaga attcatctgg attgcagcta tcttcataac gataatatta
      481 gttgatttct actgaattat taagagcatc ctgactctcg atcgtttcgg caaggtaatc
      541 ttgacaatca tcaaaaaatg acaaggcaac ttcattcgtt ggtatcaatt caatttttgg
      601 gttttcccaa ggtccattaa tagagtagta attaacacca acttccttga gtggttttct
      661 cagtaatttt gtaaatataa agacactggc cgctacagca ggccctccga gcattgcacc
      721 ccccatgggt aacatatccg aaattgctgg ttgaactatc gccaattggt taaaagttct
      781 accaatcaaa tcagtcgctc cgatgatcac aatatcagca gttgtcccct tgatcacaag
      841 atcgcaagta taagcactac cattattaaa ttgaaaatca gcagttactt ttttaaagct
      901 aaagcctttc ttagtgatat cactaaaatc caacaacagc cttttaggta atttagaaag
      961 actaaataag ctaaaaattt ttccgggaac tcgctcaaga agggtaatct gaccatcatc
     1021 aatattaatt ttcatactcc cttccccatg aattatttgt ttcctgcgtg gtatttctga
     1081 ccaaatcata tctagatcaa attcagaatt attactggct ataaagcttt gaaggccaaa
     1141 ttgctcttgt agcgtttcta tatcaacact ccttgctttt acatttttta aagtgagcag
     1201 aggtcttctt gcacctgggt aagataaaac aatcagtttt tttatcggta tctgatcaaa
     1261 catgaaccca ttttctggtg aatgtgtgat caatatctca ctgccatcaa ttaaaagtga
     1321 ttttattctg gttgatctag atttgaatga ggcaagaaaa tcaatctcaa tttctacaat
     1381 attggcctgc agtcccttgt cttcctgttg aaataattcc atcgagaatt gatcaaaagt
     1441 taaaatagga ttaatagaaa aatcattaat ggtcagccac ttaaaatcca agtcttgata
     1501 gtcaatgaca gcatcgaata ccaaattcat ccgagcaata acagggtttt tccaactaga
     1561 tatgtacttt ggtaaaagaa tgctcgaggt cgccacaaca gttagaaaaa gcatgacgat
     1621 agtaactaaa caaattttta tgattctagt catagggaat atatatctaa tatttatagt
     1681 aaattacaat accatcttct tctaatcacg aatcagatga aagtcattca tgacctaatt
     1741 tcgatggtac ggttgtcctt ccaaaatagt ccaagctcta tatatttgct cggctaaaat
     1801 ggctggaaca aaggcatgtg gcaaagttaa tctggacaat gaaagacatc tgtctgctcg
     1861 ctgatgaaca agatctgaga atccatcggg tcctccaatt gccataacgg gcttggttga
     1921 ctgcaacatc catttttcaa tcaataagga aaactctttt gtagaaagca tttcgccgtc
     1981 ctcgtcaagc aatatcagtg tttcatcttt ttttataaga ctcagtaatg ctttaccttc
     2041 ttggtttaag gcatgatcaa tcggttgatt tttttttcgt tgagctaaag gtatcttatt
     2101 tagatgtagt tggatatgtg gtggtagtct tttttggtaa actgaaaaac catcatctaa
     2161 ccaagctatt gatttagatg caatcgcatg cattttaatt ttcataattg aggtctaact
     2221 gtttgatcgc ccacactcca taaatcctcc aacttgtaga atgaacgagt ttcctcctgc
     2281 ataatatgga caatgatatc cccaagatca attaataccc attgcgaagt ctcataacct
     2341 tccacaccca acactgcatg accctctttc tttacacttt cagcaacctt gtctgcgata
     2401 gagcggacat gccttgacga attacctgtg gcaaagatca tgtaatcact aatactcgtt
     2461 atggaggaga catctatact tgaaactgcc tttgctttca tatcattaag cactgtcaaa
     2521 gttaatttaa gcagttcttg gtgcatcacg ttaaatggtt tcaggggtag aaaacatgaa
     2581 taataagtat ccaatttctt cccagggatt accaatgacc acacctttta tgacattatc
     2641 gaccttgcat gcctgatcaa ccaattggcc aatttgagtt ttggattggc taatcagata
     2701 aggttcaatt attttttgtt tgctgggcca aattctagcc ttctgcatgg cttgtttgac
     2761 cgataaacca ccatcgatat ccaaagaagc atccattaag ttctttattt cccaatataa
     2821 aacagccaga atttgctccg gtctcatccc ttcacttttt aagcgttcat aaatacgata
     2881 aactcttgtc ggattcttca aaaaagccgc ttcggttagt tggaataagt caaaatgagc
     2941 tccatcagcc acacattgag aaatattttc catcgtcact ttgcctgtaa cttccatcat
     3001 cagtagtcgt tcaatggact gttttgcaac gataagattg ccttcagtca ataatacaaa
     3061 catttctaag gcgtctcgat cgatacctaa accatgtctt gttaatctgt ttgttagcca
     3121 accacgaaat tgtcttggat aaacttttgg acactcaata ctgattgcct ttttctgcaa
     3181 ttcttttacc cacttggctt gcttgatatc tttttcaagt ttgcctgcta cgaatactag
     3241 taatgtgtcc tcataaagat tctcaattaa tttaacaatt gcttcagagc cttgcttacc
     3301 aggcctagaa ttgggtattt gtatttctat atattttttc cctccaaata gagactggct
     3361 ttttaaacta tcatctactg caccccattt gaaccgacct gtaactgtga aagtagcctt
     3421 atcgatgtaa tcatttaatg ctgctgcttt tctcaattca tcaagagact caatgatgag
     3481 taaatcttca gatcctgaaa ggaaataaat agaatggacc ttttctgaaa gatgattgat
     3541 taattcgcta ctactgagtt tcaaatatcg atctccttga gttcgattgc agcatcatct
     3601 agcaacaaag gaatatcatc ttcaattgga tataacagtg ttttactaga attgattaga
     3661 gcattgttca ctgtatcttt tgcaaccgat ccatctagtt ttttgagtgt tttgtttcca
     3721 atggctgtat taataacttg aagttctttt gatgtcacaa aactcagttt ttctctgaaa
     3781 actgggcaac aaataatgtc taataatttt ggatccatat gttaagaatt ttttggacta
     3841 gagaaaaaac gaaatgtctc cggaacggga tcgtagccag aattaaacaa aggattacaa
     3901 cgaaaaagcc tagtcagtga cagagtcatt cctttcatca acccaaaacg atccaaagct
     3961 tccagtgcat actgggagca agtcggttgg aaacgacaag aaggtttaaa aaaaggagac
     4021 aataattttt gataagcaat aataactgat tttgccaatt gttttggagc aatcataata
     4081 gttatcatca atttacttta gttttaatct tttggctagc aaccatgtta tttgcgtcat
     4141 tcttgattct ttccagcatg atatttaatc cattgttccg ggtgggactt aagtgttcat
     4201 caagaccaat catcgatata aaagatggct tggtatga
//
LOCUS       DASCTH010000236         2779 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000450611, whole genome shotgun sequence.
ACCESSION   DASCTH010000236 DASCTH010000000
VERSION     DASCTH010000236.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000450611"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="QGH35_04995"
     CDS             <1..422
                     /locus_tag="QGH35_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81924.1"
                     /translation="YFTPYLNAFMEENADIVFPVNTAYESSGSFINVSGIVQNFTVGA
                     SQHINRPSNSDLLNNIISSLELPEVSTIDLDQKTKSFLDQSVSEKQTISELPTTNINK
                     PENRLRTYSLYNVDSTVRRSIPLQETSDAKSLQPEEH"
     gene            424..1494
                     /gene="nuoH"
                     /locus_tag="QGH35_05000"
     CDS             424..1494
                     /gene="nuoH"
                     /locus_tag="QGH35_05000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HJL81925.1"
                     /translation="MEEFFINLFSFIPSNYMTLVISLMKILIVTVTLLLIIAYLIYFE
                     RKVIGYMHVRIGPNRTGPRGWLQSIADILKLLIKEIIIPSSADRFLFLVAPLLSLVPA
                     LAAWAVIPFSESFIIADIDAGLLYLLALTSFGVYGIILAGWASNSKYAFLGAMRATAQ
                     MVAYEIAMGFALVGVLMAGGSLNLGVIVESQSGGVLNWFWIPLFPLFIVYFISGVAET
                     NRHPFDVAEGESEIVAGFNVEYSGIAFGIFFCAEYANMILIATLTSIMFLGGWESFFA
                     GTSFLNGTIFEVITEPNIFWLAVKVSIFLFIFFWLRASLPRYRYDQIMKLGWKVLVPI
                     TILWIGVEGAMYLAHIGPWKIT"
     gene            1491..1976
                     /gene="nuoI"
                     /locus_tag="QGH35_05005"
     CDS             1491..1976
                     /gene="nuoI"
                     /locus_tag="QGH35_05005"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006459418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HJL81926.1"
                     /translation="MTIKHIIKTLTLTDIVKGLVITLKNFFTTKITVQYPEVKTPLSP
                     RFRGKLALMRYDNGEERCIACKLCSAVCPAQAITIESEEQTDGTRRTTKFEIDAFKCI
                     YCGFCEQACPVDSIIETDIFEYHMTENEQRIQTKEMLLKTGEDFRERTLTNKIKDAQY
                     K"
     gene            1979..2587
                     /locus_tag="QGH35_05010"
     CDS             1979..2587
                     /locus_tag="QGH35_05010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HJL81927.1"
                     /translation="MTSLELIFYIFSSILLLSSLGVVLFKNPVYSALSLISCFITSAF
                     LWLLLEAEFLALVLVLVYVGAVMVLFLFVIMMLNIELEVETSQFNKIAPLGLIVGLII
                     VGELMTLIWLRSDQFLVTSSAVSSFDKEVSNSVLLGTKLFTDYIYAFEISGFILLLAI
                     IVSISLTLRRRKGLKRQIVSKQVDIDPATRVKLVDMESEVNK"
     gene            2584..>2779
                     /gene="nuoK"
                     /locus_tag="QGH35_05015"
     CDS             2584..>2779
                     /gene="nuoK"
                     /locus_tag="QGH35_05015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HJL81928.1"
                     /translation="MITLQSYLTLAALLFAIGAMGIIINKKNIIIVMMSIELMLLAVN
                     FNFIAFSYFSNDLTGQIFVFF"
BASE COUNT          837 a          464 c          530 g          948 t
ORIGIN      
        1 tttattttac cccctacttg aacgcattca tggaagagaa tgcagacatt gtgtttccag
       61 ttaatactgc ctatgaatcg agtggaagtt ttattaatgt atcaggcatt gttcagaatt
      121 ttactgtggg agctagccaa catatcaata gaccatccaa tagtgacctt ttgaataata
      181 ttatctcatc attggaactg cccgaggtat caacaataga tttagatcaa aaaaccaaga
      241 gctttttgga ccaatctgtc agtgagaaac aaactatcag tgaacttcca acaacaaaca
      301 taaataaacc tgaaaacaga ttaagaactt atagtttata caatgtagat tcaaccgtta
      361 ggagatcaat accacttcaa gaaacaagtg atgcaaaatc tttacagccc gaggaacatt
      421 aaggtggaag aattttttat taatctattc agctttattc caagcaacta tatgacctta
      481 gtgatcagtt tgatgaaaat cttaattgtg accgttactc tattgttaat tattgcctat
      541 ttaatctatt ttgaaaggaa ggttattggc tatatgcacg ttagaattgg gccaaacaga
      601 acaggtccca gaggatggtt acaatctatt gcagatatct taaaattgct tataaaagaa
      661 atcattatcc cgtcgagtgc agatcgcttc ttatttttgg ttgcaccatt actctctcta
      721 gtacctgcat tggcagcatg ggcagtgata ccctttagtg aaagttttat catagctgat
      781 attgatgcag gtcttttata cttactcgct ctgacctctt ttggggtata tggaataatt
      841 cttgcaggat gggcctcaaa ttcaaaatat gcattcttag gtgcaatgcg tgccacagca
      901 cagatggttg cttatgagat tgcaatgggg tttgcgttag ttggggtttt aatggctggt
      961 gggagtctta atttaggagt cattgttgag agtcaaagcg gtggagtttt aaactggttt
     1021 tggattccgt tattcccatt atttattgtt tattttattt ctggagtagc cgaaaccaat
     1081 agacatcctt tcgatgttgc agaaggtgaa agtgaaattg ttgcaggctt caacgtagaa
     1141 tattctggta ttgcattcgg aattttcttc tgtgctgaat atgcaaatat gattctcatt
     1201 gcaaccctaa cctcaattat gtttttaggc ggttgggaga gtttctttgc tggaacttca
     1261 ttcttaaacg gaacaatatt tgaagttata acagaaccaa atattttctg gctggcagta
     1321 aaagtatcca tctttttatt tatatttttt tggttgagag cttcattgcc tagatataga
     1381 tatgaccaaa ttatgaagct tggttggaag gttctagtcc ccattactat cctatggatt
     1441 ggagttgaag gggctatgta tttagcacat atcggtcctt ggaagatcac atgactatta
     1501 aacatataat caaaactcta acacttactg atatagttaa aggtctcgtt ataaccttaa
     1561 aaaacttctt tacaacaaag attactgttc agtatccaga agtaaaaaca ccactttcac
     1621 ctagatttag gggcaaactg gctctcatga gatacgacaa tggtgaggaa cgttgtatcg
     1681 cttgtaagtt gtgctctgct gtttgtcctg ctcaagcaat aacaattgag tcggaagaac
     1741 aaacagacgg cacacgaaga actactaaat tcgaaataga tgccttcaaa tgcatttatt
     1801 gtggcttctg tgaacaagca tgccctgttg attctattat tgagaccgat atttttgaat
     1861 accatatgac tgaaaatgaa cagagaattc agaccaagga aatgttatta aagacgggtg
     1921 aggatttccg tgaaaggacc cttacaaata aaataaaaga tgcacaatat aaataatcat
     1981 gacatccctt gaattaattt tttacatctt ttcatcaatc ttattattat caagtttagg
     2041 tgttgtgtta tttaagaacc ctgtttactc tgcactttca ttgatctcgt gttttataac
     2101 cagtgctttt ttatggctat tactagaagc agaattctta gctcttgttc ttgtgcttgt
     2161 atatgttggt gcggtcatgg ttctattctt gtttgtcatt atgatgctga atattgaact
     2221 cgaggtcgag acttcacaat ttaataagat tgctcctttg ggattaattg tcggtctcat
     2281 catcgtcggt gaattaatga ctctaatctg gcttcgctcg gatcagtttc tggttactag
     2341 cagtgcagtt tctagcttcg ataaagaagt tagtaactca gtattattag gaacaaagct
     2401 gtttacagac tatatttatg catttgagat ttctggattt atattattat tggcaatcat
     2461 tgtttcaatt tctctcacct taagaaggcg aaaaggactt aagagacaaa tcgtctccaa
     2521 acaagttgat attgatccgg caactagagt taagttggtg gatatggaga gtgaggtcaa
     2581 taaatgataa ctttgcaatc ctatttaact ttggctgctc tgcttttcgc aattggtgca
     2641 atgggtatca ttatcaataa aaagaacatc atcattgtaa tgatgtccat agaattaatg
     2701 ctactggcag taaattttaa ctttatagct ttttcctatt tcagcaatga tctcaccggt
     2761 caaatatttg tatttttta
//
LOCUS       DASCTH010000237         5046 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000453570, whole genome shotgun sequence.
ACCESSION   DASCTH010000237 DASCTH010000000
VERSION     DASCTH010000237.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000453570"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="QGH35_05020"
     CDS             <1..886
                     /locus_tag="QGH35_05020"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HJL81929.1"
                     /translation="NRQIYRNMLFTSAGISDYISGVILFEETLRQSNDTGVAFPEYLN
                     SLGIIPGIKVDKGAKELAGCSGEQVTEGLDGLRERLSEYYSLGARFAKWRAVITIADG
                     IPSDTCIGVNAHALARYAALCQESAMVPIVEPEVLMDGEHSLETCYDITAKVLDCVFS
                     ELVNQKVMLNGIILKPNMIISGTDAANRADVAMVASATVDCLTAHVPKDVPGIAFLSG
                     GQTSEEATAHLSAMNELGPHPWQLSFSYGRALQAFPLKVWAGENENIASAQVEFLKRA
                     KLNGLARTGSYNPQMESA"
     gene            901..1503
                     /locus_tag="QGH35_05025"
     CDS             901..1503
                     /locus_tag="QGH35_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017319.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJL81930.1"
                     /translation="MVSSKIKSLILALLLICTFPVLGSETLSPYEMITTSINLLKVEL
                     GDEVNKQRLEENRTELYSIIDESLSPYFHKRYAGRLVLDQHWAGASGEQRRRFTEGLY
                     QTLIESYAITMLNFNISKINVLPHNNDLENVKKTTVQSEVDYKGELIPMNFKFGKFKE
                     GWLFYDVRIEGVSYIKNYRNQYGAEIQENGLESVIIRLEN"
     gene            complement(1500..2240)
                     /locus_tag="QGH35_05030"
     CDS             complement(1500..2240)
                     /locus_tag="QGH35_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HJL81931.1"
                     /translation="MLNHSFLKIIFSFSLSLFVLSGCSYQNVLTNDEKDPLEAVNRNF
                     YVFNDNLDKVVIEPTADGYDFVTPSVFQTGVNNFFDNFQYPITIANQLLQGNAKLASQ
                     DTLRFIINSTIGFAGLFDPAKNMGFTKHDEDFGQTLAIWGVGEGPYLMLPFLGPYTLR
                     DLTGTLIDYVFDPWTYADGMSRKIKLKAIESLQKRADLSTIEHELDNSYDPYKYIKDS
                     YFQNRSYKINNGPFLEEDEEDLIEFDDF"
     gene            complement(2241..3704)
                     /locus_tag="QGH35_05035"
     CDS             complement(2241..3704)
                     /locus_tag="QGH35_05035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HJL81932.1"
                     /translation="MDKFFLNIFILFFCFFQARISYSEIELPDLGNPSDIYMSKMEEP
                     AVGRAYYREMLKKDQVVEDALLQEYIQHLGEKLSSKVQEGDFKFNYFLVPQNTINAFA
                     LPGGFIGINLGLVLNADTESQLASVLAHEISHVTQRHISRQIGNNVPNSIQGGLVLLG
                     AILLGAISGSSEVLEAGTMLAPSIMQQGQIDFTREMEIEADATGIKTLALSGYDPYAM
                     ADFFNKLSAGDDPRNAASIEFLRTHPTSVNRAASARKQARKLTVKNIDDSLVFELSKA
                     RIRYLFSNTLEKANFYFKSNTAGIDITQDNLSYKQIGNLYGLALTSIDLGQFNQAENI
                     LRPLLYTYEEYNHFHIAYAHLLTQQGQHKDAINYLKKIMALSPRNIPITLDYSLNELN
                     YGDTQTAHKTLLDLFNNVVPSPSQIKLIAKAANAAEDYADSFSYMAEYYLSIGGFKEA
                     IEQLQLALSIDSINDIQKSKFIARLQEIEDFIAENED"
     gene            complement(3721..4188)
                     /locus_tag="QGH35_05040"
     CDS             complement(3721..4188)
                     /locus_tag="QGH35_05040"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015780646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJL81933.1"
                     /translation="MAKVKVGTKVPDFRATLTKGFEFVLSEYLGKNIIVYFYPKDNTQ
                     GCTKESESFRDHFQSFCSNNTEIVGVSRDSIQSHEKFIHKYALPFDLISDEDEKVCQL
                     FEVIKEKNMYGKKYMGIERSTFIIDVNGILIHEWRKVKVNNHVEEVLNTLEQM"
     gene            4422..>5046
                     /locus_tag="QGH35_05045"
     CDS             4422..>5046
                     /locus_tag="QGH35_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81934.1"
                     /translation="MRIKHLLIVTFSTLSGCSFFEGSVQTCNEPQEYQESISVPALIV
                     PDSLKELQNRSIFNIPDVLKTKSFNQISNNTPKISNNKTSSNKTINKIENIEDDELSE
                     LLELIDQTIEYRTLREYNLAYDSSVIDSDVEESLGPCAEDPPNYFAEGVMPRSMPSQT
                     YSKSSNRSKKTKTEKSRRQKRKEARQQRKTDQQSEAATAEDTSSENAA"
BASE COUNT         1653 a          854 c          924 g         1615 t
ORIGIN      
        1 aaacaggcaa atttatcgca acatgttatt cacttctgca ggcatttcag actatatcag
       61 tggtgtaata ttatttgaag aaactctgag acaatcaaac gatacaggtg ttgctttccc
      121 agagtattta aattctttag gaattatacc tggcattaaa gttgacaaag gggcaaaaga
      181 gctagcggga tgttcaggtg aacaagttac tgaaggactg gatggactca gggaacggct
      241 atctgaatat tattctttag gcgcaagatt tgcaaaatgg agagctgtga tcacaattgc
      301 tgatggaatt ccaagtgata catgcattgg ggtgaatgca catgctttgg ctaggtatgc
      361 tgcgctctgt caagaatcag ccatggttcc aattgttgag cctgaagtct tgatggatgg
      421 cgaacacagt ttagagactt gttacgatat taccgcaaaa gttttagatt gtgtgttttc
      481 agaacttgtg aatcaaaaag ttatgctgaa tggaattatt ttaaaaccaa acatgatcat
      541 ttcaggaacc gacgctgcta atcgtgctga tgtcgctatg gttgcaagtg ccacggtaga
      601 ttgtttgaca gctcatgtac cgaaagatgt tccagggatc gcttttttat caggcggaca
      661 aacctctgaa gaagcgacgg cgcatcttag tgccatgaat gaattgggac cacatccttg
      721 gcaattatct ttttcatacg gtagggcact gcaagccttt ccacttaaag tttgggcagg
      781 ggagaacgaa aacatagcct cagctcaggt agagttccta aaaagagcta agctcaatgg
      841 actggcaaga accggaagtt ataatccgca aatggaatcc gcttaatttg gaagcaattt
      901 atggtatcaa gcaaaattaa atcccttatc cttgcgttgc tacttatatg caccttccct
      961 gtattgggtt cggaaacatt atccccctat gaaatgatta caacatcaat taatctgttg
     1021 aaagtagagc taggagatga agtcaataaa cagagactgg aagaaaatag aacggagtta
     1081 tacagtatta ttgatgagtc attaagtcct tattttcata aaagatatgc cggcagattg
     1141 gttttggatc aacattgggc gggtgctagt ggagaacaaa gaaggagatt cacagaaggt
     1201 ttgtatcaaa ctttgattga gtcctacgca atcacgatgc taaattttaa tatttccaag
     1261 atcaatgtat tacctcacaa taacgattta gaaaatgtaa aaaaaacaac cgttcaatct
     1321 gaagttgatt acaaaggtga actcatacca atgaatttta aatttggaaa gtttaaagaa
     1381 ggctggttgt tttatgatgt gcgtatcgag ggtgtgtcgt atataaagaa ttatagaaac
     1441 caatacggtg ctgaaattca ggaaaatggt ttggaatcgg tcatcattag actggaaaat
     1501 tagaaatcat caaattcaat caaatcttct tcatcctctt cgaggaatgg tccattattt
     1561 attttgtatg atctgttctg gaaatatgaa tctttaatgt atttgtaagg gtcataagaa
     1621 ttatccagtt catgttcaat agtggataaa tctgctcttt tttgaagact ctcgattgct
     1681 tttagtttaa ttttcctact catgccatcc gcataggtcc atggatcaaa aacataatcg
     1741 atcagtgtgc cggttaaatc tctgagtgta tatgggccca aaaaaggaag cattaaataa
     1801 ggtccttctc ctactcccca gatagctaag gtttgaccaa aatcttcgtc gtgtttggta
     1861 aagcccatat tttttgctgg gtcaaacaaa ccagcaaaac cgattgttga gttaatgatg
     1921 aaacgaagag tatcttgtga tgctaatttg gcattgcctt gaagcagttg attggcaata
     1981 gtaattgggt attgaaagtt atcaaaaaag ttattgaccc cagtttgaaa gacactaggt
     2041 gttacgaaat catatccatc agcagtaggt tcaatgacga ctttgtctaa attatcattg
     2101 aatacgtaga aattacggtt gactgcttcc agaggatcct tttcatcgtt tgtcaaaaca
     2161 ttttgatagc tacagcctga gagtacaaaa agagataagc taaaagaaaa tattattttt
     2221 agaaaagaat ggtttaacat ttaatcctca ttttctgcaa taaaatcttc tatttcctgg
     2281 agtctagcta taaattttga tttttgaata tcattaatag agtcaataga caaagccaat
     2341 tgtaactgtt caatagcctc tttaaaaccg cctatgctta gatagtattc agccatatat
     2401 gaaaatgagt cggcatagtc ttcagcagca tttgctgctt ttgctattaa cttaatttgc
     2461 gaaggagaag ggactacatt attgaataaa tccaataagg ttttgtgtgc tgtttgagta
     2521 tcaccataat tcaattcatt taaagaataa tctagtgtta tcggtatatt tctcggagag
     2581 agcgccatga ttttttttag atagttaata gcatctttat gttgtccctg ttgagtcagt
     2641 agatgtgcat aggcaatatg gaaatgattg tattcttcat aagtataaag aagtggtctt
     2701 aatatgtttt ctgcttgatt aaattgaccc aaatcaatgg aggtaagcgc taatccgtat
     2761 aaattaccga tttgtttata actgagattg tcttgagtga tatctattcc agcggtattt
     2821 gatttaaagt aaaaattggc tttttctaag gtattactaa atagatatct aattcttgcc
     2881 ttgcttagct caaaaaccaa tgaatcatca atatttttta cggtcaattt tcttgcctgt
     2941 ttacgtgcag aggcagcacg gttaactgac gttggatggg ttcttagaaa ttcaattgat
     3001 gcagcatttc ttggatcatc tccggcagat aatttattga aaaaatcggc cattgcatat
     3061 ggatcatatc ctgacaatgc taatgtttta ataccagtag catccgcttc aatttccatt
     3121 tctcttgtaa aatcaatttg accttgttgc ataatcgaag gagctaacat agtacctgcc
     3181 tcaagaactt cactacttcc agatattgca cctaataaaa tagcacctaa gagaactaaa
     3241 cctccttgga ttgagttggg tacattgttg ccaatttgtc tggaaatatg acgttgcgtt
     3301 acatgactga tttcgtgtgc tagaactgag gcaagttgac tctctgtatc agcatttaaa
     3361 actaggccta aattaatccc gatgaaacca ccaggcaatg caaaggcatt gattgtattc
     3421 tggggtacta aaaagtaatt aaatttaaaa tcaccttctt ggaccttaga tgatagcttt
     3481 tcccccagat gctgaatata ttcttgaagt aatgcgtctt cgactacttg atcttttttc
     3541 aacatttctc ggtagtatgc tcttcctact gccggttctt ccatttttga catgtaaata
     3601 tctgaaggat ttcctaaatc ggggagttca atttctgaat agctaatcct tgcttgaaaa
     3661 aaacagaaaa agagtatgaa aatattgaga aagaatttat ccatactttg aaagcatctc
     3721 ttacatctgc tcaagggtat ttagtacttc ttcaacatgg ttattaactt ttacttttcg
     3781 ccattcatga atcaaaattc catttacatc aatgatgaat gtacttcttt caatgcccat
     3841 atatttttta ccgtacatat ttttttcctt aatcacttca aatagttgac atactttttc
     3901 atcttcatct gaaatcaaat caaatgggag agcatattta tgtataaatt tttcatgaga
     3961 ttgtatcgag tctcttgata cacccacaat ttcagtattg ttggaacaaa atgactgaaa
     4021 atgatctcta aatgattcac tttctttagt gcatccttga gtgttatctt ttggataaaa
     4081 ataaacaatg atgtttttcc ctaaatactc acttagaaca aattcaaaac cttttgttaa
     4141 tgttgctctg aagtcaggta cttttgttcc aacttttact ttagccatat acgctcctct
     4201 aaatataaaa catcaaagtt ctttatgaag ttaatatcat aatcgcttca ttttatatta
     4261 aaatatcaat cttttttgtt cttagtattt tgccacttca aaaaatggaa tgattgctat
     4321 ctgaattaag aagtagttaa aactaaaaaa aagagcaggg aactaaagtt taatttgtaa
     4381 taatgtagga actaactaac agtaaataaa tgaacgcctc aatgagaata aaacatctat
     4441 taattgtaac tttttctact ttgtcaggat gcagtttctt tgaaggttca gttcagactt
     4501 gtaatgaacc tcaagaatac caagaaagta tttctgtgcc tgcattgatt gttcctgatt
     4561 ctttaaaaga gctacaaaac agatctatat tcaatatccc agatgttctt aagaccaagt
     4621 catttaatca aataagtaac aatacaccta aaatctctaa caataaaacc tcatctaata
     4681 aaacaatcaa taaaatagaa aatattgaag atgatgaatt atcagagctg ttggaattga
     4741 tagatcaaac cattgaatac agaacactta gagaatataa cttggcatat gatagctctg
     4801 taatagattc tgatgtagaa gaatcattag gaccatgcgc agaagaccct ccgaattact
     4861 ttgctgaggg ggtgatgcct aggtccatgc catcacagac ttatagcaaa tcttctaatc
     4921 gttctaaaaa aacaaaaaca gaaaaatcac gtcgacaaaa acggaaagaa gctaggcaac
     4981 aaagaaaaac tgatcagcag tcagaagcag caactgcaga agatacttct tccgagaatg
     5041 cagcag
//
LOCUS       DASCTH010000238        10038 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000456209, whole genome shotgun sequence.
ACCESSION   DASCTH010000238 DASCTH010000000
VERSION     DASCTH010000238.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10038
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000456209"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="QGH35_05050"
     CDS             complement(<1..643)
                     /locus_tag="QGH35_05050"
                     /inference="COORDINATES: protein motif:HMM:NF023358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 domain-containing protein"
                     /protein_id="HJL81935.1"
                     /translation="MLNIYRFRVATTMFLMLSVLLLPRSNSAQEIPEAEFILPDPMEI
                     LEGSLIVQRTSSLGSKDSQTTVSDLSIETQELLGEYRLVLQQTDRLIAYNDYVERLIT
                     DQEAQIIDINGQLEKFAMIERGIVPLMLDSIETLDKFIDLDVPFLLEERKARVARLRI
                     IMDQSNITVSEKYRQIMDAYQIETSFSRDIEAYTGFLDIDGENHQVDFLRIGRT"
     gene            complement(718..1185)
                     /locus_tag="QGH35_05055"
     CDS             complement(718..1185)
                     /locus_tag="QGH35_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81936.1"
                     /translation="MIEKVLPSEANNNYQGHKIALYGLILMTGLMTWRSCIHLFFAEY
                     GMHSIAHFIVFEGVPDPNLVNYLFFSLWGLQQLLFCLINSIILFRYRNLIPLMFVLWL
                     LEWSLRPLLVGNLHPLGEQYFTGTTPGIIGTPYVVIFLIVMLILSLTPSKRLS"
     gene            complement(1209..2861)
                     /locus_tag="QGH35_05060"
     CDS             complement(1209..2861)
                     /locus_tag="QGH35_05060"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HJL81937.1"
                     /translation="MTVKRESMEFDVVIVGAGPAGLSASIKLMQLSQEKSLDLNVCVL
                     EKGSDVGAHIISGAVIETRALDELLPDWESNDPFKKTAVTKDQMSILINQASSINVPT
                     FLIPGAMNNHGNYITSVGSLCRWLAAKAESMGVNIFPGFPAVELIEEEGAIVGVISGD
                     MGLSKDGQQKEGYEPGYELRAKYTIFSEGCRGNLGKQLIKRFELDAHSDSQHYGIGFK
                     EVWNITPEKHQLGSVLHTAGWPLDSSIEGGGFVYHAQDQLLYLGLIVSLDYQNPNLSP
                     FGEFQKWKSHPKIKNLLEGGERISYGARAVNKGGLQSLPKLTFPGGLLVGCDAGFLNG
                     AKIKGTHTAMKSGMLAAESIVDSFSSDVQENLLEGFEKSVQQSWVYQELYRARNFSPA
                     QKRFGLYLGSIFIWLDQNIFRGRLPMTLHVQEPDYSKSRLLAESKSIDYPKPDGVTTF
                     DRTTSIYLSGVAHEEDQPCHLYLTDPNIPIETNLPKFGEPAQLYCPAGVYELVESSDG
                     ELQFQINAQNCIHCKTCDIKDPSQNIVWKCPEGGGGPNYTDM"
     gene            3001..3744
                     /gene="gpmA"
                     /locus_tag="QGH35_05065"
     CDS             3001..3744
                     /gene="gpmA"
                     /locus_tag="QGH35_05065"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HJL81938.1"
                     /translation="MPGKLVLCRHGQSEWNQDNRFTGWEDVDLSPRGEEEAREAGRAL
                     KNENLPFDIAYTSVLKRATRTLWIMLEAMDLTRLPIINAWELNERHYGALQGLNKSEV
                     ASEYGEDQVQIWRRSYDTPPPLLETDDERHLKFKDQYAAHNDVPLGESLKDTLARVRP
                     YWQTIIMPSLLGNQNVLIVAHGNSLRALVKMIEQISDEDITQFNIPTGIPFVYEFNEN
                     LSPIKQGFIGDEEIIKEATEAITKQGLAK"
     gene            3751..4269
                     /locus_tag="QGH35_05070"
     CDS             3751..4269
                     /locus_tag="QGH35_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020399289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HJL81939.1"
                     /translation="MLYTLNNAIPNCEKASFIAANASVIGKVHMEQDVSIWFNAVIRG
                     DNEPIHIGKGSNIQDGSVLHTDMGAPLSIGEMVTVGHKVMLHGCTINNQCIIGMNSTI
                     LNYAVIGENSIVGANSLITENKTFPGNSLIMGSPAVVVRELTDEDLQTIMEFANHYID
                     NGKRYAKNLVTS"
     gene            complement(4259..5137)
                     /gene="tesB"
                     /locus_tag="QGH35_05075"
     CDS             complement(4259..5137)
                     /gene="tesB"
                     /locus_tag="QGH35_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HJL81940.1"
                     /translation="MSQDNTLHASIERLIELLDLERIEENIFRGESQDIGSPQVFGGQ
                     VLGQALIAASRTVENRDVHSLHAYFLRRGDFEAPIVYEVDRARDGGSFSNRRVIAVQH
                     GEQIFNMTASFQKPEEGLEHQTTMPEVPQPEELEDLRDLIKPEWVEKLPPRMQRMLKR
                     QRPFEVRPVELPYFLNNKIKEPIKHAWIRVKGLISKDFQLHQALLAYISDYNLLTTAI
                     LPHGTNFMQNRFQMASLDHAMWFHKTCLVNEWMLFAYDSPRSSGARGFATGYIFSEEG
                     VLIASMAQEGLMRIRK"
     gene            complement(5134..5601)
                     /locus_tag="QGH35_05080"
     CDS             complement(5134..5601)
                     /locus_tag="QGH35_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HJL81941.1"
                     /translation="MEHEISSFEDFKALEGHEVGVSDWFEVTQQQIDRFADLTGDFQW
                     IHVDVERAKRDLPGGKTIAHGYLVLSLTPAMTSDFMNFSNLERAINYGVNKVRFMNMV
                     PVGCRIRARSAVKQIRKRSDAVQVMGQTTVEIEGETKPACVIETVSFFYFKQL"
     gene            complement(5610..6203)
                     /gene="folE"
                     /locus_tag="QGH35_05085"
     CDS             complement(5610..6203)
                     /gene="folE"
                     /locus_tag="QGH35_05085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HJL81942.1"
                     /translation="MTENYKKPTRKEAEQAVRTLIKWAGDDPNREGLEKTPERVINAY
                     EDWFSGYQEEPYEYLTRTFKEVAGYDEMVTLRGIDFQSHCEHHIALISGKAYVGYLPD
                     KQVVGISKLARVVEAYARRFQVQEKMTAQIANCINDVLKPKGVGVVIVARHACMTTRG
                     IRKSNVDMVTSTMLGGFRKSEATRIEFMNTIGNLMLD"
     gene            complement(6308..6862)
                     /locus_tag="QGH35_05090"
     CDS             complement(6308..6862)
                     /locus_tag="QGH35_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024214.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HJL81943.1"
                     /translation="MTPLQGLILTGGQSKRMGQDKALMRSGSRTQLEQSYDLLQAQLS
                     SVYVSIKASQVNDPVRSQYRLIMDAQNCSGPMAGILSAHKTYPDCAWLVIACDMPFLN
                     SETLMQLIKARDNAYDATVFSNPDDSLPEPLCAIYEPNALVRVASDPTRLPSNSARDL
                     LRQSRIKMIEAKNPYALENTNYSR"
     gene            complement(6884..7348)
                     /gene="moaC"
                     /locus_tag="QGH35_05095"
     CDS             complement(6884..7348)
                     /gene="moaC"
                     /locus_tag="QGH35_05095"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019990887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HJL81944.1"
                     /translation="MLSHIDKNNHPKMVDISDKKTTKRIAIASAIVKLPDLVQREFKD
                     GDIQTKKGPVIDTAIVAGTMAAKKTHELIPFCHPLGLDSCKISIDSDDSGHLVICCEV
                     TIDHKTGVEMEALTGATVAALTVYDMCKSISHEIEITDIQLRKKSGGKRSVG"
     gene            7493..8902
                     /gene="leuC"
                     /locus_tag="QGH35_05100"
     CDS             7493..8902
                     /gene="leuC"
                     /locus_tag="QGH35_05100"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013753366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HJL81945.1"
                     /translation="MSLTLVEKIWREHEIVEQSDGNSLLYIDRIFLHERTGSIALKAL
                     EEANRSIRHPKQVFCTLDHIVDTFPERTDETMMPNGKEFITATRSSAQKAGIHFFDLN
                     HPSQGIVHVVSPEQGIALPGITLVCADSHTCTLGGLGALAWGIGSSNCEHALCTNTLV
                     IKKPRMMKVIFNGTLPYGVTAKDLILHLISKFGASGGQGYAVEFSGSVIEHLEIEARL
                     TLCNMAVEFGAWTGIVAPDQKTIDYVINRAFAPQGENLDEALANWVNLKSDKNAHFDE
                     VITIECDQLSPFITWGTSPEHAISIDGKIPDPAQERNNNKQQSMMQALDYMNLNPGTN
                     MQDIKIDAAFIGSCTNSRLSDLRNAAKILKNQKVAPGIKAICVPGSSQVKQQAEQEGL
                     DLIFKTAGFEWRQSGCSMCFFVGGESFDPGKRVITTTNRNFENRQGKRVRSHLASPAT
                     VAASAIAGKISNANNQGTL"
     gene            8902..9522
                     /gene="leuD"
                     /locus_tag="QGH35_05105"
     CDS             8902..9522
                     /gene="leuD"
                     /locus_tag="QGH35_05105"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HJL81946.1"
                     /translation="MKPFIEHTGIVAPLMRININTDDIIPSREITTVHREGLKDGLFA
                     EWRYISLKTREENPNFVLNQNPFKKASILLTGDNFGCGSSREHAVWAIEQWGIRVIIA
                     SSFGSIFRNNCIQNGILPISLKPNKIKSLQNFVEADPRKNQLTVNLEHSKMTTGDGTE
                     YPFEIDPSDQQKLLRGIDAIELTLENINLIKEFEHKDKKLRPWVYF"
     gene            9651..>10038
                     /locus_tag="QGH35_05110"
     CDS             9651..>10038
                     /locus_tag="QGH35_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJL81947.1"
                     /translation="MIVHGGKLAAQALKQAGVECIFTLCGGHVMAIYDGCLDEGIKII
                     DVRHEQAAVHAADAWSRLNPNKIGCAVLTAGPGVTDGVTGVANAWRANSPILVIGGQG
                     PFSNLRQGSLQEMDHVAVMKPITKWAD"
BASE COUNT         3067 a         2122 c         1929 g         2920 t
ORIGIN      
        1 aagttcttcc aattctaaga aaatcaacct ggtgattctc tccatcaata tccaaaaatc
       61 ctgtatacgc ttcaatgtct cgactaaaac tagtttcaat ctgatatgca tccataattt
      121 gtcgatattt ttcagagacc gtaatatttg attggtccat tatgatcctg agtcgagcaa
      181 ctctagcctt tctttcttcc aataaaaaag gaacatctaa atcgatgaat ttatctaggg
      241 tttcgatact gtcgagcatc agtggaacaa taccgcgttc gatcattgcg aatttttcca
      301 attgcccatt tatatcgatg atttgagctt cttgatcggt aatgaggcgc tcgacataat
      361 cattgtatgc gatcaatcgg tcagtctgtt gcaaaaccaa gcgatactca ccaagtaatt
      421 cctgtgtttc aatagaaagg tcactcacgg ttgtttgtga atctttagag cctaaactcg
      481 aagttctttg aacgatcaga ctaccctcaa ggatctccat cggatcgggt aaaataaatt
      541 ctgcctcagg aatctcttga gcagaattag aacgaggtaa gagcaataca ctaagcatga
      601 gaaacatagt tgtcgcaact ctaaatctat aaatatttaa catagcctaa atccctataa
      661 attttgttaa gtcattataa gtaaaaattc aacagaagat atcagataaa atattaccta
      721 tgaaagtcgt tttgaaggag tcagcgacag aatcaacatt acgatcaaga aaataaccac
      781 atacggggtc cctattatcc ctggagtagt acctgtaaaa tactgttcac caagaggatg
      841 aagattacct actaataagg gtctcaagga ccattcaagc agccacaata cgaacatcaa
      901 gggaataaga ttacggtatc ggaataaaat gatactgtta atcaggcaaa atagtagttg
      961 ctgcagtccc catagggaga agaacagata attgaccaaa ttagggtcag gaacgccttc
     1021 aaaaacaatg aagtgtgcaa tggaatgcat tccgtattct gcgaaaaata aatgtataca
     1081 gctcctccaa gtcatcaggc cagtcattag aatcaatcca tacaaagcaa ttttatgacc
     1141 ttgataatta ttatttgcct cactgggcag aactttttca atcatcccca tctatttacc
     1201 ctagggtgct acatgtccgt gtaatttggc ccgcctcctc cctctgggca tttccatact
     1261 atattttgac tggggtcctt aatatcgcaa gttttacaat gaatacaatt ttgagcatta
     1321 atctgaaatt gtaattcccc gtcactggat tcaacaagtt catagacccc tgccggacaa
     1381 tacaattggg caggttcgcc aaactttggc agattggtct ctattggaat attaggatca
     1441 gtcaaataaa gatggcaggg ctgatcctct tcatgagcca ctcctgataa ataaatagaa
     1501 gtggttcgat caaatgtagt tacaccatcg ggtttgggat aatcgatgga ttttgattct
     1561 gccaaaagcc ttgacttgga ataatccggc tcttgaacat gaagtgtcat gggcaatcta
     1621 cctctgaaaa tattttgatc taaccaaata aatatagatc ccaaatataa accgaatctt
     1681 ttttgagcag gtgaaaaatt tctggcacga taaagttcct gataaaccca tgattgttgg
     1741 acagattttt caaacccttc caataaattt tcttgaacat ctgaagaaaa agaatcaacg
     1801 atggattcag ccgccaacat tccagatttc atagcagtat gggtgccctt gattttcgca
     1861 ccattgagaa atcctgcatc acaaccaacc aataatccac ctggaaaggt taacttggga
     1921 agcgactgaa ggcctccttt gttaactgct ctggctccat aggaaatacg ctcccctcct
     1981 tctaaaagat ttttaatttt tgggtgtgat ttccatttct gaaactcacc aaacgggcta
     2041 agatttggat tttgataatc caaagacacg attaaaccca gatataatag ttgatcttgt
     2101 gcatggtaga caaagcctcc accttcgatt gatgagtcga gtggccagcc tgcggtatgc
     2161 aacaccgaac ccaactggtg cttctcagga gtgatattcc agacttcctt aaatcctatt
     2221 ccgtagtgtt gggaatctga atgagcatcc aactcaaatc gtttaatcaa ttgcttgccc
     2281 aaattacctc gacagccctc agagaatatt gtgtattttg ctcgaagctc atatcctggt
     2341 tcataaccct ctttttgttg tccatcctta gacaatccca tatctccaga aatcacgcct
     2401 actatagcgc cttcttcctc gatcagttca actgcgggaa atcctggaaa aatatttaca
     2461 cccatcgatt ctgcttttgc tgccaaccat cgacaaagtg aacccacgct ggtgatataa
     2521 ttcccatgat tattcatagc ccctggtatt aaaaaagtag gcacattaat actcgatgct
     2581 tggttgatga gaatagacat ttggtctttg gtaaccgcag ttttcttgaa tggatcattg
     2641 ctttcccaat ccggtaataa ttcatccagt gcgcgtgtct cgattacagc acctgaaata
     2701 atatgtgcac caacatcgga tcctttttcc agcacgcaaa cattcaaatc aaggcttttt
     2761 tcttgtgaca attgcatcag tttaatagac gcacttaagc ctgccggtcc tgcaccgact
     2821 atcacaacat cgaattccat cgattcacgt tttacagtca taacaaatgg tagtttatta
     2881 aaatctcatt ctatcaatat ttttcaagca atttggcaac caagtgtttt ttattaccaa
     2941 gagaatgtaa aattacacaa gaagtattac tgtttgagat aaatataacg aggtaatcga
     3001 atgcctggaa aactagttct ttgtcgtcac ggtcaaagcg agtggaatca agacaataga
     3061 ttcaccggat gggaggatgt agatctaagc ccccgaggcg aagaagaagc aagagaggca
     3121 ggacgtgctc taaaaaatga aaatttacct tttgatattg cttatacatc ggttctcaaa
     3181 agagccacaa gaactctatg gattatgctt gaagcaatgg atctcactag attgcccatt
     3241 attaatgcat gggaactcaa tgaacgacat tatggtgcac tgcaagggct gaataaatcg
     3301 gaagtagcaa gtgaatacgg tgaggaccaa gttcagattt ggcgaagaag ctacgataca
     3361 cctccaccgt tacttgagac cgatgatgaa agacacctaa aatttaaaga tcaatatgca
     3421 gcccacaacg atgtaccact aggtgagagt ctcaaggata ctcttgctag agtgaggcct
     3481 tactggcaaa caataataat gccctctctt ctcgggaatc aaaacgtact gatcgtagca
     3541 catggcaata gcttaagagc attggtaaaa atgattgaac aaatctcaga tgaagacatc
     3601 actcaattca atataccaac ggggatacct tttgtttatg aattcaatga aaaccttagt
     3661 cctatcaaac aaggctttat tggtgatgaa gaaatcatca aagaagccac agaagctatt
     3721 acaaaacaag gcttagcaaa ataatagaaa atgctctaca cccttaataa cgctatcccc
     3781 aactgcgaaa aggccagttt tattgctgcc aatgcatcag tgatcggtaa agtacatatg
     3841 gaacaagatg tcagtatttg gtttaacgca gtcattcgag gggacaacga accaattcat
     3901 ataggcaaag gaagtaatat tcaggatggt tccgttctgc acactgatat gggtgcccct
     3961 ttatcgattg gtgaaatggt tactgttgga cataaggtca tgctgcatgg ttgcacaatt
     4021 aataatcaat gcatcattgg aatgaatagc accatactca attatgctgt catcggagag
     4081 aactccatcg tcggtgcaaa ttccttaatt accgagaaca aaacatttcc aggaaattcc
     4141 cttatcatgg gctcaccagc ggtagtagtg cgtgaactca ccgatgagga tctacaaacg
     4201 ataatggaat ttgccaacca ttacattgat aatggcaaac gttatgcaaa aaacttagtc
     4261 acttcctgat gcgcattaag ccttcttgag ccatagaagc aattaaaacc ccttcttctg
     4321 aaaaaatata accggtagca aaccccctgg cacctgatga tcttgggcta tcgtaggcaa
     4381 acaacatcca ttcattaacc aaacaggttt tatgaaacca catggcatga tctaggctgg
     4441 ccatctggaa acgattttgc ataaaattgg tgccatgagg taggattgct gtggtcagta
     4501 ggttgtaatc agaaatataa gccaacaaag cctgatgcag ttgaaaatct ttagatataa
     4561 gtcctttgac tctaatccat gcatgtttaa taggttcctt gattttgtta ttcagaaaat
     4621 atggcaactc cactggccgc acctcaaaag gtctttgacg cttcagcatt ctttgcattc
     4681 taggtggtaa tttctccacc cattcgggtt taattaaatc acgtaaatct tctaattctt
     4741 ctggttgagg cacttccggc attgtcgttt gatgctcaag tccttcttca ggtttttgaa
     4801 aagaagcagt catattgaat atttgctcac catgttgaac agctatgact cttctattag
     4861 agaaactgcc accatctctt gctctatcca cttcatagac aattggagct tcaaaatccc
     4921 ctcgccttaa aaaataagca tgcagtgaat gcacatcacg attttcaacc gttcggctgg
     4981 ccgcaatgag agcttggccc aatacttgtc caccaaagac ttgtggactg ccaatatctt
     5041 gactttctcc tcgaaaaata ttttcttcaa ttctctcaag atcaaggagt tcgatcaatc
     5101 tctcgattga ggcatgcagg gtattgtctt gactcatagc tgtttaaaat aaaaaaatga
     5161 taccgtttct ataacacaag caggtttggt ctccccttca atttcaacgg ttgtttgacc
     5221 catgacttga acagcatctg agcgttttct gatctgcttc acagctgacc tggctcttat
     5281 tctgcagccg actggaacca tattcataaa acgcacctta ttgaccccat aattgattgc
     5341 tctctcaaga ttactaaaat tcataaaatc tgaggtcatt gcaggagtaa gagataatac
     5401 taaataaccg tgtgcaatgg ttttaccacc aggaagatct cttttagcac gttccacatc
     5461 aacatgaatc cactgaaaat caccagttaa gtccgcaaag cgatcaatct gctgttgggt
     5521 tacctcaaac caatcagaaa cccccacttc gtgaccttca agggccttaa aatcttcaaa
     5581 agatgaaatc tcgtgttcca taaataatcc tagtcaagca ttaaattacc gatagtattc
     5641 ataaactcta ttcgggttgc ttcacttttt ctaaatccac ccaacatggt gctggttacc
     5701 atatcaacat tactttttcg aatacctctt gtagtcatac aggcatgtcg ggcaactata
     5761 accactccga cacctttggg tttcaaaaca tcattgatgc aattggcaat ctgggctgtc
     5821 attttttcct gaacctggaa acgccgggca taagcttcca ccactctggc taatttgctg
     5881 atcccaacga cttgtttatc cggtaaataa cccacatagg ctttgccaga aatcaatgca
     5941 atatgatgtt cgcaatggga ctgaaaatca atccccctta gggtgaccat ctcatcataa
     6001 cctgcgacct ccttaaatgt ccgagtcaaa tactcgtagg gttcttcctg gtaaccacta
     6061 aaccaatcct cataagcatt aataactcgc tctggtgttt tttccaagcc ttctctattc
     6121 gggtcatctc ctgcccattt aatcaacgtc cgaacggctt gttctgcctc tttacgggtt
     6181 ggctttttgt agttctctgt catagtgttc ccttgataag atttcctttc attcctcagg
     6241 cattaagcaa attatcaatg tttgcgacca atgatcgcat agtcaccatt caatttcact
     6301 caaatcctta tcttgaataa ttcgtattct ccagtgcata tggatttttt gcttcaatca
     6361 tttttatcct agattgccgc aaaagatctc tagcgctatt gcttggcaac cgagtgggat
     6421 cgctggcaac cctcaccagc gcattgggtt cataaattgc acataacggc tctggtaagg
     6481 aatcgtctgg attgctaaaa acagtcgcat cgtatgcatt gtctctggct ttaatcaatt
     6541 gcataagtgt ttcagaattt agaaaaggca tgtcgcaagc tataaccaac caagcacaat
     6601 caggataggt cttgtgtgca gacaagattc cagccattgg acccgagcaa ttctgtgcat
     6661 ccataatgag tctgtactgt gaccggacag gatcattcac ctggctcgct ttaatggata
     6721 catataccga tgacagctgt gcttgcaaaa gatcgtatga ctgctccagc tgagttcttg
     6781 atcctgaacg catcaaggct ttatcttgac ccatccgttt gctttgcccc ccggtcagta
     6841 tcagtccttg caatggtgtc ataatagttg gtacggcaga tagttagcca acggaacgtt
     6901 tgcctcctga ttttttccga agctgtatgt ctgttatctc aatttcgtga gatattgatt
     6961 tacacatatc ataaactgtc agtgccgcca cggttgctcc agtcaaagct tccatttcga
     7021 cccctgtttt atgatctata gtaacttcac agcatatgac taaatgccca gaatcatcag
     7081 aatcaattga aatcttgcag gaatccagtc ccagtgggtg gcaaaatgga atcaactcat
     7141 gagttttttt agccgccatg gtaccggcaa caatggctgt gtcgatcact ggaccttttt
     7201 tggtttgaat atccccatct ttaaattccc tttgaaccaa atcaggtagc ttgactatgg
     7261 cagaagcaat agcaattctc ttggttgtct ttttgtcgga aatatcaacc attttggggt
     7321 gattattttt atcaatgtga ctcagcacta catcttctta tttatttcat tgttatgaat
     7381 aattataact tgtttgtttt tttagaatca tgatttctaa gtaaatgaag actcgagaaa
     7441 tattattcat taattaattt ataatatgca tgcaatcagg aaattagaaa atatgagctt
     7501 aacgctggta gaaaaaattt ggcgagaaca cgaaattgtt gagcaaagtg atggcaatag
     7561 cctcctttat attgatcgaa ttttcttaca tgaacgcacc gggagtattg ccttgaaagc
     7621 tttggaggaa gctaatcgtt ccattcgcca tcccaaacaa gtcttctgta cattggatca
     7681 tattgttgat acattccctg agcgcactga tgaaaccatg atgcccaatg gtaaagagtt
     7741 tattacagca acccgttcat cagctcagaa agcagggatt catttttttg atttaaacca
     7801 ccccagtcaa ggaattgtgc atgttgtctc acctgaacaa ggtattgcac tgcccgggat
     7861 aaccttggtc tgtgctgatt cacacacctg caccctagga ggcttaggtg ccttagcttg
     7921 ggggattggg agcagtaatt gcgaacacgc cttgtgcacg aatactttag tgattaaaaa
     7981 accaagaatg atgaaggtca tttttaatgg cactcttccc tatggagtga cagccaaaga
     8041 tttaatatta catttgatct caaaatttgg agccagtggt ggccaaggtt atgcagtaga
     8101 attttcaggc tctgtcattg aacatttaga gattgaggcc cgtttaacac tatgcaatat
     8161 ggcggttgag ttcggagcat ggacaggtat tgtggctcct gatcagaaaa ccatcgatta
     8221 tgtcatcaat agagcttttg ctccccaggg tgaaaactta gacgaagcac tggccaattg
     8281 ggtaaattta aaaagtgata aaaatgctca ctttgatgaa gtaattacca ttgaatgcga
     8341 tcaattatca ccttttatca cttggggaac tagccctgag catgccattt cgattgatgg
     8401 gaaaatccca gatcctgctc aggaaaggaa caacaataaa caacaatcaa tgatgcaagc
     8461 gctcgattat atgaatctca atccaggtac caacatgcaa gatattaaga ttgatgctgc
     8521 cttcattggt tcttgtacaa atagccggtt aagtgatttg agaaacgctg ccaaaatttt
     8581 aaaaaaccaa aaagtagcgc ctggtataaa agccatttgt gttccaggat cctcccaggt
     8641 aaaacaacaa gcagagcaag aaggactgga tctgattttt aaaactgccg gctttgaatg
     8701 gcgacaatct ggctgttcaa tgtgtttttt tgtgggtgga gaaagctttg acccaggaaa
     8761 aagagtgatc actaccacca atagaaactt tgaaaatagg caaggaaaac gtgttcgttc
     8821 ccaccttgca tcccctgcta cggtagcagc atcagccatt gccggtaaaa tcagtaatgc
     8881 taacaaccaa ggaactttat aatgaaaccc ttcatagaac atactggtat tgtggctcct
     8941 ttgatgcgaa ttaatattaa taccgatgac atcataccca gtagagaaat aactaccgtt
     9001 catagagaag gattgaaaga tggactgttt gctgagtgga ggtatatttc attgaaaact
     9061 cgggaagaaa atcctaattt tgttttaaat caaaacccat ttaagaaagc atccatttta
     9121 ctgaccggtg ataattttgg ttgtggatcc tcgagggagc atgctgtttg ggctatagag
     9181 cagtggggca ttcgagtcat catagcttct agttttggtt ctatttttcg taataattgc
     9241 attcagaatg gtattctccc aatcagttta aagcctaata aaattaaatc gttgcagaat
     9301 tttgtagaag ctgatcctag aaaaaatcaa ctcactgtga acttagaaca ctcgaagatg
     9361 accactggag acggtactga atatcccttt gagattgatc caagtgatca acaaaaacta
     9421 ctacgaggaa ttgatgcgat tgaattaacc ctagaaaata tcaacttaat taaagaattt
     9481 gagcacaaag acaaaaaact aagaccttgg gtctattttt aattttttct ctggtgtact
     9541 cacatatctc agtttttagt agcaattaat ttctatataa tgaaatctcc aataaaaaaa
     9601 catcagcaat acaacagatt gctgaatcaa tcgtttgtga ggggagatag atgatagtac
     9661 atggtggaaa gctagcagca caagctctta aacaggctgg cgtagagtgt atctttaccc
     9721 tctgtggtgg ccatgtaatg gctatttacg atggctgtct ggatgaaggt atcaagataa
     9781 ttgatgtacg acatgaacaa gctgctgtgc acgctgcaga cgcctggagt cgattaaatc
     9841 ccaataaaat tggttgtgca gtactcacag ccggtccagg tgttacggac ggtgttacag
     9901 gtgttgcaaa cgcatggcgt gccaatagtc ccatcctagt cattggtggt cagggtccct
     9961 tcagcaattt aagacaagga tcactgcagg aaatggacca tgtagcggtc atgaaaccaa
    10021 tcactaaatg ggctgata
//
LOCUS       DASCTH010000239         1699 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000459261, whole genome shotgun sequence.
ACCESSION   DASCTH010000239 DASCTH010000000
VERSION     DASCTH010000239.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000459261"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(90..659)
                     /locus_tag="QGH35_05115"
     CDS             complement(90..659)
                     /locus_tag="QGH35_05115"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HJL81948.1"
                     /translation="MKKILLIDNYDSFTYNLVQAFLSLEAEVVVFRNDEIDLSEAALI
                     KPTHLVISPGPGRPENAGISLSAIEDFHKKIPILGVCLGHQCIVEAFGGDVIHAKSLM
                     HGKTSMINHDGKAIFSHIDNPFEAGRYHSLAADQRTIPPTLVATATTEDGEIMGVRHL
                     ELPIEGVQFHPESVLTPQGEILMSNFLQT"
     gene            complement(656..>1699)
                     /locus_tag="QGH35_05120"
     CDS             complement(656..>1699)
                     /locus_tag="QGH35_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012640.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HJL81949.1"
                     /translation="LPKSQHQVTTTEHPDGCYIAPESLLIFDHLTRRIALLHVSGEDQ
                     RSQLKDKIIELLRGPIPYSDEQHAFTAPEPNITDSEYYQAVEKAKNLIREGDIYQLVL
                     SIKFRGSCHLDPFQAYRAMRLLNPSPYMFYCDVGHFQVVGSSPEALVRLNGTQASLRP
                     IAGTRPRGKNSQEDAVLEADLISDEKENAEHVMLVDLARNDLGRVARTGTINVHPYKS
                     IEHYSHVMHMVSGVQGELGQDQDAFDLFAAAFPAGTLVGAPKVRAMELIDSIEPELRG
                     IYGGTVGYFGKNGDMDQAIAIRTIVFSEGQYSFQAGGGIVADSKPDDELKEVMAKSEI
                     LRKALEMAVEGMH"
BASE COUNT          448 a          376 c          346 g          529 t
ORIGIN      
        1 gcataagcag tggactcact taaatcattt ttgtttaaaa gttcatccaa tagttgatct
       61 gctgaggtat tcatgacgtt gcctttgttc taggtttgta aaaaattact cataagtatc
      121 tccccttgcg gggtcaagac actctctggg tgaaattgaa caccttcaat gggtaactcc
      181 agatggcgca cgcccataat ttcgccatcc tctgttgttg cagtggcgac taacgttgga
      241 ggaatggttc tttgatctgc tgcaagcgag tggtatctgc cggcttcaaa cggattatca
      301 atgtgtgaaa aaattgcttt gccatcgtgg ttaatcattg atgtttttcc atgcattaag
      361 ctttttgcat gaatgacatc accaccaaaa gcttccacaa tgcattggtg acctagacaa
      421 actcccagaa taggaatttt cttgtgaaag tcttcaatcg cagacaagga aatacctgca
      481 ttttcaggcc tgcctggtcc tggtgatatg accaagtggg ttggtttaat tagagcggcc
      541 tcggatagat ctatttcatc attgcgaaag accacaacct ctgcttccaa tgacaaaaaa
      601 gcctggacta ggttataggt aaatgaatcg taattatcaa ttaggagaat ttttttcaat
      661 gcatgccctc cacagccatc tctaaggctt ttcttaatat ttcacttttt gccataactt
      721 ctttaagctc atcgtcgggt ttgctatcag caacaattcc acctcctgct tgaaaactat
      781 actgcccttc agaaaagaca atggttctaa ttgcaatcgc ttgatccata tctccatttt
      841 ttccaaagta accaaccgtg ccaccataga tacctctgag ttctggttca attgaatcta
      901 tcaactccat ggctctaact ttaggagctc ctacaagtgt gcctgccgga aaagcggcag
      961 caaataaatc aaatgcatct tggtcttgtc ctagttcccc ctgcactccg ctgaccatat
     1021 gcattacatg actatagtgc tcaatggatt tatagggatg aacattaatt gttcctgttc
     1081 ttgctaccct gcctaagtcg tttcgtgcta aatcaaccaa cataacgtgt tctgcgtttt
     1141 ctttttcatc actaatcaag tcggcttcca atactgcatc ttcctgtgag tttttccctc
     1201 ttggtctggt cccggcaatg ggtcttagag aggcttgtgt gccatttaat ctaactaggg
     1261 cttcaggaga tgagcccact acttggaaat gacccacatc gcaataaaac atgtatggag
     1321 atggattcaa taatcgcatt gcccgatagg cttgaaaagg atccagatga cagctgcctc
     1381 taaatttaat ggacaacacc agttgataaa tatcgccctc gcggataaga ttttttgctt
     1441 tttcaaccgc ttggtagtac tcagaatcgg taatattagg ctcaggggca gtgaaagcat
     1501 gttgctcatc gctatatggt atggggcctc tgagcaactc tatgatttta tctttaagct
     1561 gacttcgttg atcttcacct gagacatgga gtaatgcaat ccttcgtgtt aaatggtcaa
     1621 agattagtaa cgactccggt gcaatatagc agccatctgg atgttctgtg gtggtgactt
     1681 ggtgttgact tttgggaag
//
LOCUS       DASCTH010000240         3113 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000462585, whole genome shotgun sequence.
ACCESSION   DASCTH010000240 DASCTH010000000
VERSION     DASCTH010000240.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000462585"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            83..715
                     /locus_tag="QGH35_05125"
     CDS             83..715
                     /locus_tag="QGH35_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015512.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HJL81950.1"
                     /translation="MTDSFIVFPKIKKIQKVWNVVLNNRLLLLIIIVFSHLFIASCAN
                     HQLVRNSELWQERLHVVNGLSEYRIKGSLSLLMNHSSFVGSFDCFKDKYSSKFIVRDY
                     FGKPVLTFDPNHPELIANDSAFDVLKNNMIFNNDNEFDILNSLLALPVNIEQDRLIYD
                     DQGWLIQVKYPEWTVHYKSYQTLNGLVIPKKITIKGRSYRLTLVNSVLEI"
     gene            782..856
                     /locus_tag="QGH35_05130"
     tRNA            782..856
                     /locus_tag="QGH35_05130"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:814..816,aa:Gln,seq:ttg)
     gene            902..1855
                     /locus_tag="QGH35_05135"
     CDS             902..1855
                     /locus_tag="QGH35_05135"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HJL81951.1"
                     /translation="MNLDNIVLFAGNASKTVGKKVAQRMMQPLGWIQCEKFSDGEISV
                     EILENIRGKDVFLIQSTCPPGNEHLMELLLMTDACRRASAKSVSAVVPYFGYSRQDRR
                     PRNTRGPISAKLVADLIETAGVERLITIDLHADQIEGFFSIPVDNVYSSPVLMGDIWK
                     QKYKDLVIVSPDVGGVLRARAIARRLDNADLAIIDKRREQANKSEVMNIIGDVEGKTC
                     VIFDDMVDTAGTLCNAAAALKSEGAIRTVSYAVHPVLSGSAVDKIQKSALDELVVTDT
                     IPLSQAALDTGKIRQLSIAELVAETIRRIIDNESVSSLYAD"
     gene            1982..2716
                     /locus_tag="QGH35_05140"
     CDS             1982..2716
                     /locus_tag="QGH35_05140"
                     /inference="COORDINATES: protein motif:HMM:NF004130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HJL81952.1"
                     /translation="MAENIDLIAELRDDSGTSSSRRSRRSGMVPAIIYGAGKESIKLN
                     IGHDQLLYKLSVPAFLTSILNIKVEDKEEPVFIKDIQIHPSKKQIIHLDFQRVIADQA
                     IRLTVPIHFIGEDLSDGVQLEGGTITPLLNEIEVSCLPKDLPEYLEVSVEGLKLDELL
                     FLSDIALPEGVEIPQLTQEEPNNEPIVAIRILIIQEEEPEEVIEEGEEGEEGTETEQD
                     EALAKTTTDSEEDTDKSDSEDHKNNS"
     gene            2732..>3113
                     /gene="pth"
                     /locus_tag="QGH35_05145"
     CDS             2732..>3113
                     /gene="pth"
                     /locus_tag="QGH35_05145"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HJL81953.1"
                     /translation="MTSAKLSPPTIFVGLGNPGEQYLKTRHNMGYWFLDLFADHHHAS
                     MFKSESRLLGSAAQVSLAERKLLLVKPSTFMNESGESIRRFLHYYKFDLSHLCVIHDD
                     LDLQYGSSKIKFDGGHGGHNGLRNV"
BASE COUNT         1000 a          517 c          638 g          958 t
ORIGIN      
        1 tgtattgttt ttttgtgtct cttcatctga taattgtttt tcaatagaac gatattgttc
       61 tactatttgc tttaacttat tgatgacaga ctctttcatt gtgtttccaa aaataaaaaa
      121 aattcaaaag gtatggaatg ttgttttaaa taaccggttg ttgttattaa tcatcattgt
      181 attttctcac ctatttattg caagttgtgc caatcatcaa ttggtcagaa attcagaact
      241 ttggcaagag agactgcatg ttgttaatgg gctctctgaa tatcgaatca agggttcttt
      301 atctctgctc atgaatcaca gttctttcgt tggttcattt gattgcttca aggataaata
      361 ttcttcaaaa tttattgtcc gtgattattt tggaaaacct gtattaacct ttgatcccaa
      421 ccatccagaa ttgatagcaa atgactcagc ttttgatgta cttaaaaata atatgatatt
      481 caacaacgat aatgaatttg atattttgaa ctcattgttg gcattgcccg taaatataga
      541 acaagatcga cttatttatg atgatcaagg ctggctcatt caagtcaaat atccagaatg
      601 gacggtgcat tataaatctt atcagacatt gaatggatta gtgatcccaa aaaaaattac
      661 gataaaaggg agatcatatc ggttgacatt ggttaatagt gttttggaaa tttagcattt
      721 tgtcaaatgc tggcttgaca gtccatataa ccaacatcag aatgattggg ttgaatgctt
      781 ttggggtgta gccaagcggt aaggcagcgg gttttgatcc cgtgatgcgt aggttcgaat
      841 cctaccaccc cagccaaaac aaaggtaata tatatgcata atcaataatc aaagtggact
      901 tatgaatcta gataatattg ttctatttgc tggcaatgct tccaaaacag tgggcaaaaa
      961 agttgcccaa cgaatgatgc agcctctagg atggattcaa tgtgaaaaat ttagcgatgg
     1021 tgaaataagc gttgaaattc ttgaaaacat aagaggtaaa gacgtgtttc ttattcaatc
     1081 aacatgtcca cctggaaacg aacacttgat ggaattactg ttgatgacgg atgcgtgtcg
     1141 aagagcatca gctaaatccg tttcagccgt tgtgccttat tttggttatt cacggcaaga
     1201 tcgtaggccc aggaatacac ggggaccaat ttctgccaag ttggtggcag acttgattga
     1261 aacagcagga gtagaaaggc tgattacaat tgaccttcat gcagatcaaa ttgaaggctt
     1321 tttttctata cctgtcgata atgtttactc atcaccagtc ttgatggggg atatatggaa
     1381 acaaaaatac aaagacctag tcatcgtttc acctgatgtt ggcggagtac tacgtgcacg
     1441 tgctattgct agaagactag ataatgcaga tttggctatt attgataaac gccgtgagca
     1501 ggccaataaa tctgaagtta tgaatattat tggagatgtc gaaggaaaaa catgtgttat
     1561 ttttgatgat atggttgata cggcagggac attgtgtaac gcagcagcag cgttaaaatc
     1621 tgaaggagcc ataagaactg tctcttatgc tgttcatccc gttctgtcag gatctgcagt
     1681 cgataaaatt cagaaatcag cattagatga actggttgta acagatacaa taccgctttc
     1741 ccaagcagct ttggatactg gaaaaattag acaattatca attgcagaat tggtggcaga
     1801 gaccattcga agaataatag acaatgagtc agtcagttct ctttatgcag actaaagaca
     1861 aaaattactt gtgaaattta aagataaaat ctagtttcaa acactaactt cggttattat
     1921 tcaccaccaa tttgctcaca attttgaatc aatacaaaac tggatgatcg gataaaaaaa
     1981 tatggctgag aatatagatt taattgcaga acttagagat gattcaggta cctccagttc
     2041 taggcgttca cgtcggtctg ggatggtacc agctataatt tatggcgcgg gaaaagaaag
     2101 cattaagtta aatataggcc atgatcaact tttgtacaaa ttgtctgtac ctgccttttt
     2161 aacaagtatt ctaaatataa aagtagagga taaggaagaa cctgttttta taaaagatat
     2221 ccaaattcat ccatcaaaaa aacaaattat tcatctagat tttcaacggg tcattgcaga
     2281 tcaggctata cgtttaacag tgccaattca ttttattggt gaagatttaa gcgatggtgt
     2341 tcagttggaa ggaggaacca tcacaccctt actgaatgaa attgaagtca gttgcttacc
     2401 aaaagacctt cctgagtatt tagaagttag tgttgaggga ctaaaattgg atgaattgct
     2461 tttcttatct gatattgctc ttcctgaagg agttgaaatt cctcaactga ctcaagaaga
     2521 acccaataat gaaccaattg tggctattcg aatcttgatc attcaggaag aagagcctga
     2581 agaagttatt gaagaaggtg aagagggtga agagggcacc gagacggaac aagatgaggc
     2641 attagcaaaa actactactg attctgaaga ggataccgat aaatctgact ctgaggatca
     2701 taaaaacaac tcctagttgg aaaggctgct aatgacttca gcaaaacttt ccccaccgac
     2761 aatttttgtc ggcttaggta atccaggaga gcaatacctg aagactagac ataatatggg
     2821 ctattggttt ctagatttat tcgccgacca ccaccatgct tctatgttta agagtgaatc
     2881 aaggcttttg ggtagtgcag ctcaggtgtc attggcagag agaaagcttt tacttgttaa
     2941 gccttctacg ttcatgaatg aaagtggcga atcgattagg cgttttttac attactataa
     3001 atttgacctt tctcaccttt gcgttataca cgatgatctt gatcttcaat atggttcatc
     3061 aaaaattaaa tttgatgggg gacatggtgg tcataatggt ttgagaaatg tca
//
LOCUS       DASCTH010000241         2067 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000463231, whole genome shotgun sequence.
ACCESSION   DASCTH010000241 DASCTH010000000
VERSION     DASCTH010000241.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000463231"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="QGH35_05150"
     CDS             complement(<1..199)
                     /locus_tag="QGH35_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013869559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HJL81954.1"
                     /translation="MPLVKPLLPETNLEVKKLAEFFNETLGFCPNSVLTMQIRPEIAT
                     AFINLNKAVMENYGRVTSALKR"
     gene            complement(190..384)
                     /locus_tag="QGH35_05155"
     CDS             complement(190..384)
                     /locus_tag="QGH35_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81955.1"
                     /translation="MIKLFYLLTFMLLASPPAEAQYRYQKDFTAEDFLKRRTAVYEEI
                     GDNLALIQGAEEVQGFIKCL"
     gene            510..1661
                     /locus_tag="QGH35_05160"
     CDS             510..1661
                     /locus_tag="QGH35_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81956.1"
                     /translation="MRFENFSKFFIFSIIGCITMMPVLIGPAIVGTLVDHGGFSEEYA
                     GWVMAFGSFGSALTLLIISVYIHRLNLKRLAYVCLFLAMIIDLYSSFNARPELYFLMI
                     RFLLGTLITVANIAVYTSIASFQNYERGYGLFILMQYSLSGLGLYYLILYSDFLGVQG
                     LYLSLAILDFIALLMVRNFPDLKMQPTSNQDSKSELKVLLTGATVFAVLGFGIHEMSG
                     VAQFTYIERIGVAISIEDQSLSKIMLIASLLGIPGSMVCIVIGKRFGLLPPFLFGFLG
                     CLLGMSLLIISKTYLTYTIRMCLMGFSWSLLLPYIQSYLASLDKKGSALAAGNSFATI
                     GAAIGAALGANLVGQNSNYDGLLQVTILIYMVAVLFFIISIKRKHLVHE"
     gene            1669..>2067
                     /locus_tag="QGH35_05165"
     CDS             1669..>2067
                     /locus_tag="QGH35_05165"
                     /inference="COORDINATES: protein motif:HMM:NF016274.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HJL81957.1"
                     /translation="MKDPRDLGLFMPGEWEKHTCCWMAWPARVDLWPNIEATKKAYAD
                     VANTIAEFEPVNLLVLPSMLDDARAYLSKNVEIIEMSIDDSWTRDSGPNFLINDSGLL
                     AGSTWEFNAWGDKFRPYDQDALMGSRILDWV"
BASE COUNT          595 a          338 c          408 g          726 t
ORIGIN      
        1 tccttttcaa agctgaagtg actcttccgt aattctccat cactgcttta tttaaattaa
       61 tgaaagcagt agcaatctca ggtctaattt gcattgttaa cactgagttg ggacaaaaac
      121 ctaaggtttc attaaaaaac tctgcaagtt ttttaacttc taaattagtt tctggtaaaa
      181 gaggttttac taaaggcatt ttataaagcc ctgaacttct tctgctcctt gaatgagagc
      241 aagattgtcg ccaatttcct cataaactgc tgtgcggcgc ttcaaaaagt cttcagcagt
      301 aaagtctttc tgataccggt actgagcctc agcaggtggg ctagcgagca acataaatgt
      361 tagcaagtaa aataatttaa tcataaaacg accttttttg gatattccct ttacttcatt
      421 aataattctt taagcctaca acaaatctta gttatatttc acaaatgtta atgaaattag
      481 aggttttcaa ttaagttata attctgagta tgcgattcga aaacttttca aaattcttta
      541 ttttttcaat aattggatgc ataactatga tgccagtttt gatcggtcca gcaatagttg
      601 gcactttggt agatcatgga ggtttcagtg aagaatatgc cggttgggtc atggcatttg
      661 gatcttttgg atcagcatta acattactca ttatttcagt ctacatccac cgcttaaatt
      721 tgaagcgact agcttatgtt tgtctatttt tagcaatgat tattgaccta tattcatcat
      781 tcaatgcaag gcctgagctt tattttttaa tgattagatt tttgctaggc actctgataa
      841 ctgtggcaaa tattgctgtg tatacttcaa tagcatcatt tcaaaactat gagcggggat
      901 acggtttatt tatcttgatg cagtattcgc tcagtggact aggtctatat tatttaattc
      961 tatactcaga ttttcttggt gttcaaggct tatatttatc tttagcgatc ttggatttta
     1021 ttgcattgtt aatggtcaga aattttcctg acctgaaaat gcaacccaca tcaaaccaag
     1081 actcaaaatc ggaattgaaa gtgttgttaa caggagcaac tgtttttgca gttcttggtt
     1141 ttggtattca tgaaatgtcg ggtgtagctc aatttactta cattgaaaga ataggtgtag
     1201 ccatttctat agaagatcaa tcactgagta aaattatgtt gattgcatct ctgcttggta
     1261 ttcctggatc aatggtatgt attgtaatag gcaaaagatt tggtttactg ccgccatttt
     1321 tatttggatt cttgggatgc ctgttaggca tgagtctctt aatcatttca aaaacttatc
     1381 taacttatac aataagaatg tgtttaatgg gatttagttg gtcattgctt ttgccatata
     1441 ttcagagcta tcttgcgagt ttagataaaa agggaagcgc ccttgctgca ggtaattcct
     1501 ttgccacaat aggggctgca atcggagcag cacttggagc aaatcttgtg ggccaaaaca
     1561 gtaattatga tggtcttttg caagttacga tattaattta tatggtcgcc gtcttatttt
     1621 ttatcatttc aatcaaaagg aaacatttgg tccatgagta atccaaaaat gaaagatcca
     1681 agagatcttg gcttgtttat gcccggtgaa tgggaaaagc atacctgttg ttggatggca
     1741 tggcctgctc gagttgattt atggcccaat attgaggcaa ctaaaaaagc ttatgccgat
     1801 gttgccaata caattgctga gtttgaacca gtgaatttgt tggttttgcc atcgatgtta
     1861 gatgatgcaa gagcttattt gtccaagaat gttgagataa ttgagatgtc tattgacgac
     1921 tcttggactc gtgattcagg accaaatttt cttataaatg attcaggact tctagctggt
     1981 tcgacatggg aatttaatgc ttggggagat aaatttagac cctatgatca agatgctttg
     2041 atgggatctc gaatacttga ttgggtg
//
LOCUS       DASCTH010000242         1505 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000463445, whole genome shotgun sequence.
ACCESSION   DASCTH010000242 DASCTH010000000
VERSION     DASCTH010000242.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000463445"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="QGH35_05170"
     CDS             <1..623
                     /locus_tag="QGH35_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013094.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HJL81958.1"
                     /translation="ITALYLANSGIGKIILNDFDSIDLSNLPRQILFNIEDINKNKAD
                     VAVEKLRTFNTAITLYSLNNKLSEVELSKVISNVDIVLDCTDNLESRLLINKVCINYK
                     KILISGAAIRFEGHVAIFRNDISISSCYNCLYSQDDDNLGDCEGSGILASVAGMVGSI
                     VANETIKVILKEESSIDNKLWIIDAKNNTNHLIKISKSKNCALCSI"
     gene            complement(709..>1505)
                     /locus_tag="QGH35_05175"
     CDS             complement(709..>1505)
                     /locus_tag="QGH35_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014674.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HJL81959.1"
                     /translation="KNLNSFKFVEKAIAYEITRQEELLNDGQEIIQETRLYDSDLDET
                     KSMRVKEEANDYRYFPDPDLLPLKIESKTIENIRSQLPELFEDKVERYVTQLKIKEDD
                     AGKIANDVELASFFEKVTKIFNADNLLVTNWILTEVLGALNRDNLTIEQLEVSPESFA
                     MLMTRINDQTISGKTAKEIFSLLIKEESDVDEIIQSHGLRQMSDASELEAIIKDVIQS
                     YPNQLQQYLEGKEKIFGFFIGKVMQQTKGRANPSIVNELVHKQLRK"
BASE COUNT          468 a          258 c          221 g          558 t
ORIGIN      
        1 ctatcactgc tctctattta gcaaatagtg gcataggcaa aattattctt aacgattttg
       61 attctattga tctttcaaat ttaccaagac agattctatt taatatagaa gatattaata
      121 aaaataaagc agacgttgcg gttgaaaaat taagaacatt taacactgca attacactat
      181 attcattaaa taacaaacta agtgaagttg aattatcaaa agtaatatca aatgtcgata
      241 ttgttttaga ttgtacagat aatttagaat ctagattatt aattaataaa gtttgtatta
      301 actataaaaa aattcttatt tcaggtgctg ccataagatt tgaagggcat gttgcaattt
      361 ttaggaatga tatatcaata tcgagttgtt acaactgttt gtatagccaa gatgatgaca
      421 atttaggaga ctgtgaaggt agtggaatct tggcatcagt tgcaggcatg gttggaagca
      481 tagtagcgaa tgaaaccatc aaggttattc ttaaagaaga gtcatcaatc gacaataaat
      541 tatggattat tgatgcaaaa aataatacca atcacttaat taaaatttca aaatccaaaa
      601 actgtgcact gtgttctatt tagtctttct gctcctataa tcttctatct gattctaata
      661 tgaaggtatt tgttttgatc ttcttacatc tgatatagct catgaatact attttctcaa
      721 ttgtttatgt actaattcat tcactattga tggattggct ctgccttttg tttgttgcat
      781 aactttacct atgaaaaatc caaatatttt ttctttaccc tctaaatatt gttgtaattg
      841 atttggatat gactgtatta catccttaat aattgcctca agctcagatg catcgctcat
      901 ttgtctcaat ccatgagatt gaataatctc atcaacatca ctttcttctt taatcaacaa
      961 actgaatatc tcttttgctg tcttccccga tattgtttgg tcattgattc ttgtcatcaa
     1021 cattgcaaaa gactctgggc taacctctaa ttgttcaatt gtcagattgt ctctgttgag
     1081 tgctcccaac acctccgtta atatccaatt ggtaacgagt agattgtcgg cattgaagat
     1141 tttggttact ttctcaaaaa acgatgctaa ctctacatca ttggctatct tgcctgcatc
     1201 atcctctttg atctttagtt gggtaacata acgctcaacc ttatcttcaa ataattcggg
     1261 caattgagat ctaatatttt caattgtctt gctttctatc ttcagtggca atagatctgg
     1321 atcaggaaaa tacctataat cattggcttc ttctttcacc cgcattgatt ttgtttcatc
     1381 gagatctgaa tcgtataacc gagtttcctg aataatctcc tgtccatcat tcaacaattc
     1441 ctcttgcctt gttatttcat atgcaatagc tttttcaaca aatttaaatg agtttagatt
     1501 cttta
//
LOCUS       DASCTH010000243         5894 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000467697, whole genome shotgun sequence.
ACCESSION   DASCTH010000243 DASCTH010000000
VERSION     DASCTH010000243.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000467697"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="QGH35_05180"
     CDS             <1..1723
                     /locus_tag="QGH35_05180"
                     /inference="COORDINATES: protein motif:HMM:NF019155.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HJL81960.1"
                     /translation="VDEFTGRTMSGRRWSDGLHQAVEAKEGLTVKEENQTIASITFQN
                     YFRLYKNLSGMTGTADTEAYEFQQIYGLEVVVIPTHKTMVRQDYSDLVYLDKKNKFEN
                     IIKDIKECRSQGQPILVGTASIENTEHISKLLDKEKIKHQVLNAKKHKQESMIIAQAG
                     KSGAVTIATNMAGRGTDIVLGGNLEAEINELGNDADTTKIDKLKAVWKKNHNKVIESG
                     GLHIIGTERHESRRIDNQLRGRSGRQGDPGTSRFYLSLEDDLLRIFGDPEKTQTMLSR
                     VGMEEEEAIESKLLTKQIERAQRKVEAHNFDIRKTLLEYDDVANDQRSVIYQLRNDIL
                     SSEEIDDVISGISSEVIQGLVNQFIDPDLPEDQWNLEGLSKAIYNEYNIQIDIETIVN
                     NDDSMEGQEIWKVVLEEYKRHYDSKEQLIGSVPMREIEKAVMLQQLDFHWREHLGAMD
                     YLRQGIHLRGYAQKNPKQEYKRESFEMFSFMMDELKNSVVSILATVRFKGEEDAEAVS
                     QQSPDMSDIELQHSVPGAPKESLPEHSDDKTNRPYVRVHAKVGRNEPCPCGSGKKFKK
                     CHGTIRQ"
     gene            1740..2144
                     /locus_tag="QGH35_05185"
     CDS             1740..2144
                     /locus_tag="QGH35_05185"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HJL81961.1"
                     /translation="MKRSKELLNVVIGIIIDTENKLLLNNRKQNTTLSGYWEFPGGKI
                     KPHETPDQALFRELFEELGIKIGITDPLDLIEYQYAEHDVILLPFRIIDYTGTPRGLE
                     GQELMWCSFNRLQNVNILPANRFLVERLLAKK"
     gene            complement(2145..2900)
                     /gene="zapD"
                     /locus_tag="QGH35_05190"
     CDS             complement(2145..2900)
                     /gene="zapD"
                     /locus_tag="QGH35_05190"
                     /inference="COORDINATES: protein motif:HMM:NF018740.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HJL81962.1"
                     /translation="MSKEYLYELPLNERLRTFMRVEFISNRLNYFLELDDSWATRSII
                     HGLIEIYSILSRTDVRREVITDLDRYIYEMRKYQDSPLADEDRARELRQDLESLKVTV
                     ENSGSDYLKPLRDNEFLNSLLHRQTLPGGKAEFDMPKYKFWLDSGRTKSDIDQWVGVI
                     KPVCSSIDKLLWMIRESTEAISTVAVGGFYNHQISKNHKISLVRIFLDENTIYPEVSG
                     GKHLISIRFFNWGDSEEKWSQTKENINFRISLC"
     gene            complement(2916..3563)
                     /gene="coaE"
                     /locus_tag="QGH35_05195"
     CDS             complement(2916..3563)
                     /gene="coaE"
                     /locus_tag="QGH35_05195"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HJL81963.1"
                     /translation="MNLLKSFQHFCEMNNSLKIGLTGGIASGKSIVANLFSNLGVLVI
                     DADTISHHLTTKDKPAFEEILEHFGEGVLGNDGELDRKKLRRIIFNNKTLKNDLELII
                     HPKVSEAINKEVQESCDPYVIIMVPLLIETGYNKFVNRILVVDCALDTQIKRLIKRDH
                     ETFDSAKRIIGQQIEQKNRIKMAHDVINNDMNTTIEDLDNRVEELHQSYLELSGK"
     gene            3636..4814
                     /gene="sucC"
                     /locus_tag="QGH35_05200"
     CDS             3636..4814
                     /gene="sucC"
                     /locus_tag="QGH35_05200"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020583795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HJL81964.1"
                     /translation="MNLHEFQSKELFSKVGIPILNGKVASSVQQAKEAAQKLQGNQWV
                     IKAQIHAGGRGKAGGIHIANSLDEVEGIASKLLGSRLVTKQTDPSGLPVNLVYIETAT
                     DINHELYLSLLIDRNKESWTFIASSEGGMDIEIVAEQHPENIMNYTINPVSGIDETQL
                     ESMFNKLLLNKELKEQFISITEAIFKLSQTIDASLIEINPLIVDQNNNLVALDAKIVV
                     EDNALFRQPELKGLRDDSQEDIFEKRASENDLSYVSLGGDIACLVNGAGLAMATMDLI
                     KIHGGQPANFLDVGGGATAERVTAAFELILENPNVKAILVNIFGGIVRCDVIAEGIIN
                     AIKKTHLSLPIIVRLEGTNVKSGKLKLMESGLDIIAANDLTDAAEKVVRSALNYKELK
                     "
     gene            4811..5680
                     /gene="sucD"
                     /locus_tag="QGH35_05205"
     CDS             4811..5680
                     /gene="sucD"
                     /locus_tag="QGH35_05205"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HJL81965.1"
                     /translation="MSILVDKTSKIICQGITGTQGTFHIQQCMEYGTNFVGGVTPGRG
                     GQNHLGVPVFDTVKEAKAHTGADVSIIFVPAPHAAEAIIAAIDADIALIVCITEGIPV
                     QDMIHIKSKIDQSNTKLLGPNCPGIISPSQAKIGIMPGFIHKQGSIGIISRSGTLTYE
                     AVYQTTKNGLGQSTCVGIGGDPIKGLNFIDCLELFESDDETRGIVMVGEIGGSDEEAA
                     ALFIRNNVTKPVVAYIAGVSAPPGKRMGHAGAIVTGKEGTAQGKYKALEQAGVTTVES
                     PALIGKTMKAVIS"
     gene            complement(5705..>5894)
                     /locus_tag="QGH35_05210"
     CDS             complement(5705..>5894)
                     /locus_tag="QGH35_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81966.1"
                     /translation="CIEQYNETPAAIESLKIIADSYEKLGMTDLAESAQQIYFANIPV
                     TDIDSLVLEDQKPWYKFW"
BASE COUNT         1968 a          996 c         1196 g         1734 t
ORIGIN      
        1 tgttgacgaa tttactggga gaacaatgtc gggtaggaga tggtccgatg gtttgcatca
       61 ggcagttgaa gctaaagaag gtttaacagt taaagaggag aatcaaacca tagcatcaat
      121 tactttccaa aattacttca gactatataa aaacttgtct gggatgacag gaactgctga
      181 tacggaggct tatgaatttc aacagatata tggattggaa gttgtggtca tacccactca
      241 taaaacaatg gttcgtcagg attattcaga tcttgtttat ttagacaaaa aaaataaatt
      301 cgaaaatatt attaaagaca tcaaagaatg taggtctcaa gggcaaccca ttttagtagg
      361 gactgcatca atagaaaata cggaacatat ttcgaagtta ttagataagg aaaaaattaa
      421 acatcaggtt cttaatgcta aaaaacacaa gcaagagtca atgattatcg ctcaagcagg
      481 caaatctgga gcagtaacca ttgctacaaa tatggcgggc agaggaactg atatagtttt
      541 gggaggaaat ttagaagcag aaataaatga acttgggaac gatgcagata caaccaagat
      601 tgataagctg aaagccgtat ggaaaaaaaa tcacaataaa gttattgaat caggtgggct
      661 ccatattata ggaactgaaa gacatgaatc cagacgtatc gacaaccaat taagaggacg
      721 ctcaggacga caaggtgacc ctggaactag ccgtttttat ttatcattgg aagatgattt
      781 attaaggata tttggggatc cagaaaaaac acaaaccatg ttatctagag tggggatgga
      841 agaagaggag gccattgaga gtaagctgct gacaaagcag atagaaagag cacagagaaa
      901 agttgaagca cataattttg acataagaaa aaccctactt gaatatgacg atgttgccaa
      961 tgaccagaga tctgtcattt atcagttgag aaatgatatt ttgtcatcag aagaaataga
     1021 tgatgttata tctggtatta gttcagaagt tatccaaggg ttagtcaatc aatttatcga
     1081 cccagatttg ccagaggatc agtggaattt agaaggtttg agcaaagcaa tttacaatga
     1141 atacaacatc caaatagata tcgagactat agtcaataat gatgattcca tggaaggcca
     1201 agaaatctgg aaggtcgtgt tagaagaata taagagacat tatgattcca aggagcaatt
     1261 aattggctcg gtaccgatgc gtgaaataga aaaagccgtt atgttacagc aactcgattt
     1321 tcattggaga gagcaccttg gggcaatgga ttatttgaga caaggtattc atttaagagg
     1381 atatgcacag aagaatccta aacaagaata taagagggaa tcatttgaaa tgttttcgtt
     1441 catgatggac gaactaaaga atagtgtcgt tagcatattg gctacagtgc gttttaaagg
     1501 agaagaagat gccgaagctg tcagccaaca atcacccgat atgtcagaca tcgaattaca
     1561 gcatagtgtt ccaggcgcac ctaaagaatc attacccgag cattctgatg acaagactaa
     1621 cagaccttat gtgagggtgc atgcaaaagt gggacgaaat gaaccgtgcc catgtggctc
     1681 tgggaaaaaa tttaaaaaat gtcacgggac aatccgacaa taaaaaaatc tagatttagt
     1741 tgaagcgttc aaaagaatta ttgaatgttg tcattggtat tatcatcgat actgaaaaca
     1801 agctactact taataataga aagcaaaata caactttaag tggatactgg gaatttcctg
     1861 gaggaaaaat caagccacat gagacgcctg atcaagcctt gttccgagaa ctgtttgaag
     1921 agttaggaat aaaaattggt attaccgatc cacttgattt gattgaatat caatatgcag
     1981 aacatgacgt tatattgcta cctttcagga tcatagacta cacaggaaca ccaaggggat
     2041 tagaaggaca ggagttaatg tggtgttctt ttaatcggtt acaaaacgta aatatcttgc
     2101 cggctaaccg ctttcttgtg gaaaggctat tggcaaagaa ataactaaca caaagaaatc
     2161 cgaaaattaa tattttcttt cgtttgactc catttttctt cagaatctcc ccagttaaaa
     2221 aatcggattg agattaagtg ttttcctcca ctgacctcag ggtagattgt gttttcatcc
     2281 aagaaaattc taactaaaga gattttatgg ttttttgaga tttgatgatt ataaaaacca
     2341 cctacagcaa ccgtcgaaat tgcctccgtg ctttctctga tcatccacaa taatttgtca
     2401 atagagctgc agactggttt aatcactcca acccattgat cgatatcact tttagttctt
     2461 ccagaatcaa gccaaaattt gtatttgggc atatcaaatt cagctttccc cccaggcaga
     2521 gtttgacgat gtagcagact gttaagaaac tcgttgtccc taaggggttt taagtaatca
     2581 gagccagaat tttcaaccgt tacttttagg ctttctaaat cctgccgaag ctctcttgca
     2641 cgatcttcat cggcaagtgg agaatcttgg tattttctca tttcataaat gtagcggtca
     2701 agatccgtaa taacttctct tctaacatcc gtccgggaaa gaatggagta aatctctatt
     2761 aatccatgaa taatactccg tgttgcccat gaatcatcta actcaaggaa gtaattcaat
     2821 ctattggata taaattcaac acgcatgaac gttctaaggc gctcattcag tgggagctcg
     2881 tataaatatt cttttgacat ttatttatgg ttctcttatt ttccagataa ctctaggtag
     2941 gattggtgca gttcttcaac ccgattatct aaatcttcaa tagtggtgtt catgtcatta
     3001 ttgatgacat catgggccat tttgatccta ttcttttgtt caatctgttg accgataatt
     3061 cttttggcac tgtcaaatgt ttcatgatct cgcttaatta atctttttat ttgggtatct
     3121 aatgcacagt ctacaaccag aattctattc acaaatttgt tgtaaccggt ctcaatcagc
     3181 aatggcacca ttatgattac ataaggatca caagattctt gcacttcctt attaattgcc
     3241 tctgacactt ttggatgaat gatgagttct aaatcatttt ttaatgtttt gttgttaaaa
     3301 atgattcttc tcaatttttt tctatccagc tctccgtcat tgccaagaac accttctcca
     3361 aaatgttcta agatctcctc aaaagcaggt ttgtccttcg ttgttagatg atgagaaatt
     3421 gtatcagcat caatcactag aacgcctaag ttagagaata aatttgccac aatgcttttg
     3481 ccagaagcga ttccacctgt taaacctatt ttcaatgagt tgttcatttc acaaaagtgt
     3541 tgaaaggact ttaataaatt catactttag atcaaaatac tttactttag ttttattaga
     3601 ctttcactgt ttcaattaat taatatctaa cttgtatgaa tttacatgaa tttcaatcta
     3661 aggagctctt ctcaaaagtt ggtattccta ttttaaatgg aaaagttgcc agcagcgtcc
     3721 agcaagcaaa agaagctgct cagaaattgc agggtaatca atgggtaatc aaagctcaga
     3781 tacatgccgg aggtaggggc aaggctggag gcatccacat agcaaatagc ttggatgagg
     3841 ttgaaggtat tgcttctaag ttgcttggct ctaggttggt tacaaaacaa acagatccat
     3901 caggactgcc cgttaatctt gtttatatag aaactgcaac agacatcaat catgaactct
     3961 atttaagtct cctcattgat agaaataagg aatcttggac ttttattgca tcatctgaag
     4021 gtggtatgga tattgaaata gtcgcagagc aacatccaga aaatataatg aattatacaa
     4081 ttaatcctgt tagtggaatt gatgagactc aattagaatc aatgtttaat aaacttcttt
     4141 taaataaaga gttaaaggaa caatttatat ccataacaga ggcaattttc aaactatcgc
     4201 aaactatcga tgcaagcttg attgaaataa atcccttaat tgtagatcag aataataatt
     4261 tggtagccct agatgcaaaa attgtcgtgg aagataatgc tcttttcaga caaccagaat
     4321 tgaaaggtct tagagatgat agccaggaag acatctttga aaaaagggca tcggagaatg
     4381 atcttagtta tgtttcatta ggtggggata ttgcatgttt ggtgaatgga gcagggcttg
     4441 caatggcaac tatggatctc atcaaaatac atggtggtca accggcaaat tttcttgatg
     4501 ttggaggagg cgcaactgca gagagggtta ccgcagcttt tgaattgatt ctagagaacc
     4561 ccaatgtaaa ggctatttta gttaatatct ttggtggtat tgtgaggtgt gacgttattg
     4621 cggaaggcat tattaatgcc atcaagaaaa cgcacttatc actgcctatt attgttcgac
     4681 tagaaggaac caatgttaaa tctggtaaat taaaattaat ggaatcaggt ttggatatca
     4741 ttgcagctaa tgacttaacc gatgcggctg aaaaagtagt tcgatccgcc ttaaattata
     4801 aggaattaaa gtgagtatat tggttgataa aacatcaaaa attatctgcc aagggataac
     4861 tggtacacag ggaacatttc atatacaaca atgcatggaa tatggaacta atttcgtcgg
     4921 aggagtaact ccaggtagag gaggtcagaa tcatcttggt gttcctgttt ttgacactgt
     4981 gaaagaagct aaagcacata ccggtgctga tgtcagtata atctttgttc cagcaccgca
     5041 tgctgctgaa gcaataatcg cagctattga tgcagatatt gccttgatag tttgtattac
     5101 tgaaggcatc ccagtgcaag acatgattca tattaagagt aaaattgatc aatcaaacac
     5161 taaattactg ggacctaatt gtcccggtat tatctctcca agccaagcta aaataggaat
     5221 catgcctggt tttattcaca aacagggctc aataggaatc atttccagat ctggcacttt
     5281 aacttatgaa gcggtttatc agaccaccaa gaatggatta ggtcagagca cttgtgtggg
     5341 tattggaggt gatcctatta aaggtcttaa tttcattgat tgtttagaat tatttgaatc
     5401 cgatgatgaa actagaggca tagtgatggt tggagaaatt ggaggttccg atgaggaagc
     5461 agccgctctt tttattagaa acaatgtaac caaacctgta gtagcttata tcgcaggagt
     5521 ttccgcacca ccaggaaaaa gaatgggaca tgcaggtgcc atagtcactg gtaaagaagg
     5581 tactgctcaa ggaaaatata aagctttaga acaagcaggt gtcacaacag ttgaatcgcc
     5641 agcactcatt ggtaagacga tgaaggcagt gatcagctga atacatcgtc taaaaaagat
     5701 tgaattacca gaatttatac caaggttttt gatcttccag aacaagacta tcaatatcgg
     5761 ttacagggat atttgcaaag taaatctgtt gagccgattc agctaaatct gtcattccaa
     5821 gtttctcata agaatccgct ataattttta gtgattctat tgctgccgga gtttcattgt
     5881 attgttcgat acaa
//
LOCUS       DASCTH010000244         3609 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000471996, whole genome shotgun sequence.
ACCESSION   DASCTH010000244 DASCTH010000000
VERSION     DASCTH010000244.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000471996"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="QGH35_05215"
     CDS             complement(<1..254)
                     /locus_tag="QGH35_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81967.1"
                     /translation="MKRALIVPPLLTVYGLVGQYILHIYLPITATEVLPLWLGILIIL
                     FGLFLVVYTLWIFKKAETAIIPNGTPSTLIDDRVFRFSRNP"
     gene            complement(251..1579)
                     /locus_tag="QGH35_05220"
     CDS             complement(251..1579)
                     /locus_tag="QGH35_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL81968.1"
                     /translation="MVKTTDPSLKIGPILFSPWIKFSNVLTLVYASFMWLTTVSFINF
                     VQPYLLEAALQVPENIQGRVSGNLTILQEIILIIVGGIAGAYSDKVGRRIIFASGFSF
                     MAVGFFVYPLASSLTQLFLFRSIFAIGVGMGGVMFSACIQDTPQEGSRGRWIGVIGVF
                     TGLGALFMALVLSKIPSWLITLGYTPTQAGTYACWFAASLSVLTAAVLSVGIGNPPQS
                     EKKEKRTVFSDFLVGLAQVRNPRLALAYFSAFIGRGDLLVIAVFFSLWFVRDGADLSV
                     SPEISIAKAGILFGIIQLSALLWGPVIGFISDKINRVHAVIVAYSIATVAYLLMGLYG
                     SGDPSQVTSLKMLLFAVLLGIGEISVINAGGALLGQETPPSIRGSVMGVFTVLGAAGI
                     IFASSVGGILFDNWHRGGPFVMMSILNGLMLVFALSLVYLKLTKPRTHQQ"
     gene            complement(1585..2448)
                     /locus_tag="QGH35_05225"
     CDS             complement(1585..2448)
                     /locus_tag="QGH35_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014698.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HJL81969.1"
                     /translation="MAAYKHIDVQPLSAYLGAEIKGVDITKPLQQAVVTEIKKAWQHY
                     LVLFFQDQELKPEQHISFAQYFGEPQKPGFVPTLKEFPYIRHQEFNKNPDKKMTKIGG
                     ANLTWHHDDSFWENPSKGSILHALDVPKLGGDTLFVSMIAAYNALSSEMKSILDNLTC
                     YHDVTFHARDAIIEDLGIQAFERIRKENPPVEHPIVVTQPNTGKKALYIDEMHASLIK
                     GMHPEESKYLLEFLYRHTSKPEFQCRIQWKKNAVTMWDNLCVKHRGVHDFGTAHREMQ
                     RVALVGEQRPR"
     gene            complement(2596..2850)
                     /locus_tag="QGH35_05230"
     CDS             complement(2596..2850)
                     /locus_tag="QGH35_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HJL81970.1"
                     /translation="MNIYVGNIAWSMTEDELNALFSEFGTVSSAKIITDKYSGRSKGF
                     GFVEMDDDAAGESAIEALNDSDQNGRNLRVNQARPREDRD"
     gene            3116..>3609
                     /locus_tag="QGH35_05235"
     CDS             3116..>3609
                     /locus_tag="QGH35_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HJL81971.1"
                     /translation="MMSLIESTLKEIKASFGNDYVITDLESLTFYSTDVFKQADEIAV
                     AVVRPAAADELISIAQICIKNEVPLVTRGGGASYTGGYLPSLKNTLIIDTGLLTNIDI
                     NQEDMYVTVEPGVTWLELHETLKSLGLRTPFWGPFSGRVATVGGAMSYHAISHGTNNC
                     LSADS"
BASE COUNT         1128 a          778 c          701 g         1002 t
ORIGIN      
        1 gggtttcttg aaaaccgaaa gactctgtca tcgattaacg tgcttggcgt accgttagga
       61 atgattgctg tttctgcttt tttgaaaatc cataatgtgt acacaaccaa gaataatcca
      121 aatagaatga ttaaaatacc gagccacagt ggcaagactt cggtggcagt gattggtaaa
      181 tagatatgca aaatatattg acctaccaat ccatatacag ttaaaagagg cggtactatg
      241 agagctcttt tcattgctga tgtgtccgag gtttggttaa cttgagataa accaacgaca
      301 aggcgaaaac caacatcagt ccgttgagaa tggacatcat cacgaacggg cctccacgat
      361 gccagttatc aaacaatata cctcccacag aagatgcaaa aataattcca gctgcaccaa
      421 gaactgtgaa cacgcccatc accgatccac gtatactggg aggtgtttcc tgacccagaa
      481 gtgcgcctcc tgcattgatt acactaattt cacctatccc taaaagaacg gcaaatagaa
      541 gcattttcaa tgaagtcacc tgactaggat ccccactgcc atataaaccc atcaacaggt
      601 aggccacggt tgcaatagag taagcaacga ttacagcatg tacccgatta attttatcgg
      661 aaatgaaccc tatgacggga ccccaaagta gagcggaaag ttgtatgatg ccaaaaagta
      721 tacccgcttt ggcaatgctg atttcaggac tgactgaaag atcagcgcca tcacgtacaa
      781 accataggga aaaaaacacc gcgataacga gtaaatcacc cctacctata aatgcagaaa
      841 aataagccag agccagtctt ggatttctga cttgagccag gcctaccaga aaatctgaaa
      901 aaactgtcct tttctctttt ttttcactct gtggtggatt gccaatacca acagatagta
      961 cagcagctgt caagacgctc aacgatgctg caaaccaaca agcataggtt ccagcctgtg
     1021 ttggggtata acccagtgtg atcaaccaag atggaatttt tgacaacacc aaagccataa
     1081 ataacgcacc cagtcctgta aataccccta taacgccaat ccatcgaccc cgagaacctt
     1141 cttgtggtgt atcttgaata caggctgaaa acataacacc gcccatgcct acacctatgg
     1201 caaagataga cctgaataaa aataattgtg tgagtgaaga tgccaatggg tagacaaaga
     1261 aaccaacagc catgaatgag aagcctgatg caaaaataat ccttcgacct actttgtcag
     1321 agtaggcccc agcaatgcct ccaacaatga ttagaatgat ttcttgaaga atagttaaat
     1381 taccgctgac tctaccttga atattttcag ggacttggag ggctgcctcc aataaatacg
     1441 gttgtacgaa gttgatgaag gaaacagttg tcaaccacat gaaactggca taaacaaggg
     1501 taagaacatt actgaatttg atccatggcg aaaacaagat aggaccaatt ttcaaagatg
     1561 gatctgttgt tttaaccatt atgactacct gggtctttgt tcaccgacca aagcaactct
     1621 ttgcatttct cgatgggcag taccaaaatc atgcacccct ctatgtttca cacacagatt
     1681 atcccacata gtgactgcat tctttttcca ttgaatacgg cattgaaatt ctggttttga
     1741 agtgtgtcga taaagaaact ctagtaaata tttactctcc tctggatgca tgccctttat
     1801 gagtgaagcg tgcatttcat caatataaag tgcctttttc ccagtattgg gttgtgtcac
     1861 aacgattggg tgttccaccg gtgggttttc ttttctaatt ctctcaaaag cctgaatacc
     1921 caagtcttca ataatcgcat ctcgggcatg aaatgtgacg tcgtgataac aagttaggtt
     1981 atcaagaata gatttcattt cactagaaag cgcattgtag gcagcaatca tgctgacaaa
     2041 caaagtatcc ccccccaatt ttggtacatc aagagcatgc aatattgagc ctttagatgg
     2101 gttttcccag aaagaatcat catgatgcca ggtcagattt gctcccccaa ttttggtcat
     2161 ttttttgtca ggattcttat tgaattcttg atgcctgatg tatggaaact ctttgagcgt
     2221 tggaacaaaa cccggctttt gtggctcacc aaaatattga gcgaatgaga tatgctgctc
     2281 gggttttagt tcttgatcct gaaaaaataa aacaaggtag tgttgccacg cctttttgat
     2341 ctctgtaacc acagcttgct gcaaaggctt ggtgatatca acgcctttta tttcagctcc
     2401 tagataagct gacaaaggtt gcacatcaat gtgtttatat gccgccatgt ggattactac
     2461 tatagctttt tgttatcgac aatcaaataa cgagaaaaaa ataatactaa ttttttatac
     2521 acacaggact aatcctgacc agcaatcgac tggccatgtg tgtaaacgat gaaaaaggat
     2581 tgggaaattc cggattcagt ctctatcttc tctaggtcta gcttgattaa ctcttaagtt
     2641 tctgccgttc tggtcgctat cattaagagc ttctatagct gattcacctg ctgcatcatc
     2701 atccatttcg acaaatccaa aacctttaga gcgtcctgaa tacttatcgg tgatgatttt
     2761 tgctgaggaa acagttccaa actcagaaaa aagagcgttg agctcgtcct cagtcattga
     2821 ccaggctata ttgcctacat atatattcat ctttaattga tccgtatggt taaataaagt
     2881 gtgtatatta atcttaaatc aagctaaatc aagcgataag taagtttttt tgattttttt
     2941 taattacaaa aactccttga caatctctac ccatgcctct agctctccac tcattgacaa
     3001 tatcatgagt gcctctaacc agctcagtgt aagtgaaata aattgattaa ataatctaac
     3061 atgacactaa accaaaggat ataatcgatc cttcaaaacg cgtaattacc gataaatgat
     3121 gagcttaatt gaaagcactc taaaagaaat taaagcaagc tttgggaatg actatgtcat
     3181 aactgatttg gagtcgttaa ccttttattc aaccgatgta tttaaacaag ccgatgagat
     3241 tgctgttgca gttgtgagac ctgctgctgc cgatgaattg atttcaattg cacaaatctg
     3301 catcaagaac gaagtcccac tggttaccag agggggtggt gcctcctata ctggtggcta
     3361 tttacccagt ctaaaaaata ctttgatcat tgatacagga ctattaacca acatcgacat
     3421 aaaccaagag gacatgtatg tcaccgtcga accgggcgtg acctggttgg aactccatga
     3481 aacgttgaaa tctttggggc tgagaacacc tttttgggga ccattttctg gtcgagttgc
     3541 aacagtgggt ggggcaatgt catatcatgc catcagccac ggtaccaaca actgtttatc
     3601 ggcagattc
//
LOCUS       DASCTH010000245         2746 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000475746, whole genome shotgun sequence.
ACCESSION   DASCTH010000245 DASCTH010000000
VERSION     DASCTH010000245.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000475746"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="QGH35_05240"
     CDS             complement(<1..428)
                     /locus_tag="QGH35_05240"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HJL81972.1"
                     /translation="MRSDIEIARAAKIQPIKDIALKLDIPNEYIEPYGKFKAKVNLSI
                     NHEKLKDHNNGKLILVSAITPTPAGEGKTTTTVGLSDAMALLGHKTATCIREPSLGPC
                     FGMKGGGAGGGFSQVIPMEDINLHFNGDFHAITSAHNLLAA"
     gene            complement(449..522)
                     /locus_tag="QGH35_05245"
     tRNA            complement(449..522)
                     /locus_tag="QGH35_05245"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(486..488),aa:Lys,seq:ctt)
     gene            complement(533..608)
                     /locus_tag="QGH35_05250"
     tRNA            complement(533..608)
                     /locus_tag="QGH35_05250"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(573..575),aa:His,seq:gtg)
     gene            complement(684..1112)
                     /locus_tag="QGH35_05255"
     CDS             complement(684..1112)
                     /locus_tag="QGH35_05255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJL81973.1"
                     /translation="MIIAKITTEKGDIRINLFEEDAPGTVKNFVDLANDGFYDGLNFH
                     RVIQNFVIQGGCPNGTGTGGPGYHISCEINPKKHLTGSLSMAHAGKDTGGSQFFICHS
                     PQPHLDGLHTVFGQTEDMDVVHSIEEGDGIISVSVEESAD"
     gene            complement(1259..1759)
                     /locus_tag="QGH35_05260"
     CDS             complement(1259..1759)
                     /locus_tag="QGH35_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004580666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HJL81974.1"
                     /translation="MTDKNESLGIIEKSGGRYIATLERAIEHKPEKVWSMLVESSRFI
                     DWLAPGEIEPCKGGVAKLDFVDSGIVIDSKITAFEQDQLLEYSWSGPGEPERPIRWEL
                     KPEGDITRLTLILKCPLDDDIARSCAGWEAHLMMLLAAIEGVPIKFPFERFLETREIY
                     TEMILE"
     gene            complement(1866..>2746)
                     /locus_tag="QGH35_05265"
     CDS             complement(1866..>2746)
                     /locus_tag="QGH35_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013088.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HJL81975.1"
                     /translation="NNRVYIGILFALFAVFLLCCLDVIAKILLLKYSVMQLTFLRSGF
                     ALLIVGISNYLSNGMGCFRTEIKRWHLLRTLLTTISTITFFVALGMIPLVNVIIIVFI
                     APILMTALSGPILGEHVGPWRWLAVIIGFVGMLITLKPTLGFNGQGTIYALIGVITYA
                     LVSLTSRKLSNQDSAYNLTFYMFIGPLIIGGLGSLNTWIAPTPLDWLYFVLTGVTGGA
                     AFIFYNLAFQRAEASLLASFEYTGLIWATLAGYLIFKESVEFSVWIGALIIISGGLII
                     IYRESNTLKSIKDYIP"
BASE COUNT          897 a          583 c          461 g          805 t
ORIGIN      
        1 gctgctaata gattatgagc tgaggtaata gcatgaaaat caccgttaaa atggagatta
       61 atatcttcca ttggaataac ttgagaaaaa cctccaccgg ctccaccgcc cttcatacca
      121 aaacacggtc ctaatgatgg ttctctgatg caagtggctg ttttatgccc aagcaatgcc
      181 atagcatcac ttagaccaac agttgttgta gttttacctt ctcctgcagg agttggggta
      241 atagcagaaa ccagaattaa tttaccgtta ttatggtcct taagcttttc atgattaatt
      301 gaaagattaa cttttgcctt aaatttgcca taaggctcta tgtattcatt aggaatatca
      361 agcttgagag caatatcctt aatgggttga atcttggctg ctctagcaat ttcaatatca
      421 gatctcataa tatattttcc tcatattttg ggccgtatag gaatcgaacc tataaccaat
      481 ggattaagag tccactgctc tacctaattg agctaacggc ccaataatac tatggggtgg
      541 acgatggggc tcgaacccac gaccacctgg accacaacca ggagctctac cagctgagct
      601 acgcccacca tagattttct cacaacagta ttttaactcg aattatcaag aatcagtaac
      661 aatataaata tagaaatttc tagctaatca gctgattctt caacgctgac cgaaattatc
      721 ccatcaccct cttcgattga atgcacaaca tccatgtctt ctgtttgacc aaagactgta
      781 tgcaatccat caagatgtgg ttgtggtgaa tggcaaataa agaattgact tccgcctgta
      841 tccttgcctg catgagccat tgaaagtgat cctgtaagat gcttttttgg gttaatctca
      901 caagaaatat gataaccagg tccgccagta ccggttccat ttgggcaacc gccttggatg
      961 acaaaatttt gaataactcg atgaaaattt aagccatcgt agaaaccatc attagctaaa
     1021 tcaacaaagt ttttaacagt gccaggtgca tcttcctcga aaagattgat tcgaatgtcc
     1081 cccttctcag ttgttatctt tgcaatgatc atccttttaa ttctccttat ttgataattc
     1141 tattataaga gttgtaagag tccgttgttc ctgaaaaaaa aagagcgatc attctaacag
     1201 aaagactttt agaacaataa tggttatcgt gattaaatgt tttggataag ctgttccgct
     1261 actcaagaat catttctgta taaatttccc ttgtttcaag aaagcgttca aaaggaaatt
     1321 taattgggac cccttcaatt gctgctaata acatcatcaa gtgagcttcc caacctgcgc
     1381 atgatcgagc gatatcatca tctaaaggac acttcaatat cagagtgagc cttgtaatgt
     1441 ctccttcagg tttgagctcc catctaatag ggcgctccgg ttccccgggt ccactccacg
     1501 agtattccag taattggtct tgctcaaaag cagtaatctt actatcaata acgattccac
     1561 tatccacaaa atctagttta gccacccctc ctttacacgg ttctatttca ccaggagcta
     1621 accaatctat gaaacgagat gattctacta acatagacca taccttctct ggtttatgct
     1681 caattgctct ctctaaagtg gcaatataac gtccaccaga tttctctatg attcccaatg
     1741 attcgttttt atccgtcata gcaacttaat atctccaata tttcttgata cccaaagcct
     1801 aacaaatctg tcttaattca taaaccttaa tgaaagatat ttatgtggct atttttctat
     1861 ttttattaag gaatataatc ctttatagac ttcaatgtat ttgactcacg ataaatgatg
     1921 attaaccctc cagagataat aataagggcg ccaatccaaa cactaaactc tacagattct
     1981 ttaaaaatta aataaccagc aagtgtagcc caaattaatc cagtgtattc aaaagatgca
     2041 agcaatgagg cttccgctct ctgaaatgct aagttataaa aaatgaatgc agcaccccca
     2101 gtgacaccgg ttagaacaaa gtataaccag tccaatggag ttggagctat ccatgtattt
     2161 aaacttccta atccaccaat tattaacggg ccaataaaca tataaaaagt cagattgtaa
     2221 gctgaatctt gatttgataa ttttctcgag gttaaagata ccaatgcgta agtaatcaca
     2281 ccgattaaag catagatagt tccttgaccg ttgaagccta aagtaggttt taaagtaatc
     2341 agcatcccaa caaaacctat aatgactgcc aaccatcgcc aaggaccaac atgttctccc
     2401 aggattggac cagaaagtgc agtcatcaag ataggtgcaa taaacacgat aatgatcaca
     2461 ttcactaagg gtatcattcc caaggcaaca aaaaatgtta tcgttgatat tgtcgttaaa
     2521 agagttctaa gaaggtgcca tcgttttatt tcagttcgaa aacagcccat gccattactt
     2581 aaataattac tgatacccac gattagtaaa gcaaagcctg aacgtaagaa ggtgagttgc
     2641 atgactgaat atttaaggag tagaattttt gcgatgacat ccaaacaaca caacaaaaat
     2701 actgcgaaga gagcaaacaa aataccaata taaactctat tgttag
//
LOCUS       DASCTH010000246         2768 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000477598, whole genome shotgun sequence.
ACCESSION   DASCTH010000246 DASCTH010000000
VERSION     DASCTH010000246.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000477598"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="QGH35_05270"
     CDS             <1..198
                     /locus_tag="QGH35_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HJL81976.1"
                     /translation="DLLFSVVNIARYSQLDPENALRQSNRKFETRFGKMEEKLLATDQ
                     KMENLSFTEMDRLWEQVKSQE"
     gene            202..2040
                     /gene="uvrC"
                     /locus_tag="QGH35_05275"
     CDS             202..2040
                     /gene="uvrC"
                     /locus_tag="QGH35_05275"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HJL81977.1"
                     /translation="MNPLVSERFSILKKYSRQLSSKPGVYCMVNDKETIVYVGKAKNL
                     RKRVGSYFSQTKNNQKSQAILSATYEIHVTITRTEREALILENSLIKEHKPRFNILLK
                     DSKSYPFIEVSLSDEFPRFSFHRGKQDSKGAVYFGPYPNVNALRDTLSQLQKIFRLRN
                     CEKSFFNHRSRVCLQHQIKRCSGPCVGMIDKKDYTFDVNQGLEYLKGNNRFVIDEFLK
                     KMDQASNNKEYEKAAFYRDQVSSLKMIQAHQFADGKIPIDLDAVALSYDLDMFCISVL
                     FVRGGRIMGSKNFFPTKTKLAGEEEVLESFLMQYYLEHDVPNEVIINHKIDHLETIQE
                     VLSEKTNHSVQIKLRVRMDRKQWIKIAATNSQEALTTKLAIRSTIRSQLLSLSELLEL
                     EDEIIKIECFDISHTTGDKCVAACIAFNGEGFEKKSYRRFNISGITPSDDYAAIKQAV
                     SRHYKKALKANDFLPDIVMIDGGKGQLNVAREALMELGINEIPLIAIAKGIRRKSGEE
                     KIFLNEQQIPLFLEKHSPARHLLQKIRDEAHRFAITGHRAKKRKTLLTSDLESIEGIG
                     PVKKKNLIRQFGGIQEVKRASVEDLMTINGINKPLANRIYNFFNSS"
     gene            2076..2636
                     /gene="pgsA"
                     /locus_tag="QGH35_05280"
     CDS             2076..2636
                     /gene="pgsA"
                     /locus_tag="QGH35_05280"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HJL81978.1"
                     /translation="MNSTYNVANLLTFVRIVCIPLIVIIFFLPTDLSRPITAVIFIVA
                     SITDWLDGWVAREFNQSSKLGAFLDPVADKLIVSVALILIVQSDPTILNSIIAAIVIG
                     REITVSALREWMSELGARHQVSVIGYAKLKTVLQMFGLSFMLYQIPIFGLPIYTIGQL
                     LLVVAAILTLWTMLVYLTKAWPVIIK"
BASE COUNT          906 a          502 c          550 g          810 t
ORIGIN      
        1 gatcttcttt ttagtgttgt caatattgct agatactccc aacttgatcc agagaacgcc
       61 ttaagacaat caaaccgaaa atttgaaact cgattcggaa aaatggaaga aaaacttctt
      121 gctacagatc aaaaaatgga aaatttatct tttaccgaaa tggacagatt gtgggagcag
      181 gttaaaagtc aggaatagaa aatgaaccct ctcgtttctg aacgattttc aattctaaaa
      241 aaatacagca ggcaattatc ctcaaagcct ggcgtttatt gtatggtcaa tgataaagaa
      301 acgatcgtct atgtcggtaa agcaaaaaat ttgagaaaac gagtaggcag ttacttcagt
      361 caaactaaga acaaccaaaa atctcaagca attctttcag caacttatga gattcatgtc
      421 accatcacca gaaccgagag agaagctttg attttggaaa atagtttaat caaagaacac
      481 aaacctcgtt ttaatatttt actcaaagac agtaaaagct atccctttat agaggtgtca
      541 ttaagcgacg aatttcctcg tttttcattc catagaggca agcaggatag taaaggagcc
      601 gtgtattttg gtccatatcc caatgtcaat gctctcagag acacactcag ccaattgcag
      661 aaaatattta ggttgagaaa ttgcgaaaag tcgtttttta atcatcgatc tagagtctgc
      721 ctacaacacc aaattaaaag atgctcagga ccttgtgtgg gtatgattga taaaaaagac
      781 tatacctttg atgtcaatca gggtctcgaa tatttgaaag gcaataatcg ttttgtcatc
      841 gatgaatttc tcaagaaaat ggatcaagcg tctaacaata aagaatatga gaaagctgcc
      901 ttttataggg atcaagtcag ttcattaaag atgattcagg cccaccagtt tgctgatggc
      961 aagatcccga tcgatctgga tgccgtggca ttgtcctatg atttggacat gttttgtatt
     1021 tcggttctgt ttgttcgtgg cggtcggata atgggaagca aaaacttttt tcctacaaaa
     1081 acaaaattgg caggtgagga agaagttcta gaatcttttc tgatgcaata ttaccttgaa
     1141 cacgatgttc ctaatgaagt gattatcaac cacaaaattg atcacctaga aactattcaa
     1201 gaagtgttgt ctgagaagac gaatcattca gtgcaaatca aactacgggt gagaatggat
     1261 cgaaaacagt ggatcaaaat cgcagctacc aactcccagg aagccttgac aacaaaattg
     1321 gcaatcaggt caaccatccg atctcaatta ctatccttgt cagaactatt agaactggaa
     1381 gatgaaatta tcaagattga atgctttgat attagccata cgaccggtga taaatgtgtt
     1441 gcggcgtgca tcgcttttaa tggtgaaggg tttgaaaaga aatcgtacag gcgctttaat
     1501 atttcaggaa ttactccaag tgatgattat gcagcgatca aacaagcagt gtctcgtcat
     1561 tacaaaaaag cattgaaagc aaacgatttt ttacccgata tagttatgat tgatggaggt
     1621 aaggggcaat tgaatgttgc cagagaagca ttaatggaat taggcatcaa cgagatacct
     1681 ttgattgcga tagctaaagg tattcgaagg aaatcgggag aagaaaaaat cttcctcaat
     1741 gagcagcaaa ttccattgtt tttagagaag cattctcctg cgagacactt gttacaaaaa
     1801 ataagagacg aggcacatcg ctttgcgatc actggtcacc gggcaaagaa aagaaaaaca
     1861 ttgttaacat cggatttaga atccattgaa gggattggcc cagttaagaa aaaaaacctc
     1921 atcagacaat ttggaggcat tcaggaagtc aaaagagcct ctgttgaaga tttgatgaca
     1981 atcaatggca tcaataagcc tttagcaaat agaatttata atttttttaa ctcaagctga
     2041 tctttcttcg tcaatccata ctataatctg tgaacatgaa tagcacatac aatgtagcca
     2101 atcttctgac ttttgtccgg atcgtttgta ttcctctaat cgttattatt ttttttctgc
     2161 ccactgatct ttcaagacct ataactgcgg taatttttat agtagcttcc atcaccgatt
     2221 ggttagatgg ttgggtagca agagaattta atcaaagttc gaaactgggt gcatttctag
     2281 atccagtggc tgacaaattg attgtttctg tggctttaat tttaatcgtg cagagcgatc
     2341 ccactatttt gaacagtatc attgcagcta tcgttatagg aagagaaatt accgtttctg
     2401 cattgagaga gtggatgtcg gaactgggag cccgtcatca ggtttcagtc atcggttatg
     2461 ccaaactcaa aaccgttctg caaatgtttg gtctatcctt tatgctttat caaataccta
     2521 ttttcggact ccctatttat acaatcggtc agcttctatt agtcgttgct gccattctaa
     2581 ctctgtggac tatgctcgtt tatcttacaa aggcatggcc cgttatcata aaatagtgac
     2641 ccaagatcat gaaagataga atcattataa ttcttgttat ttgcctactg cccatcactg
     2701 cttttgccaa agatgaacct ttggatgtat ttgaaagaat catggaagag caatggcaaa
     2761 ggaggctc
//
LOCUS       DASCTH010000247         1955 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000482279, whole genome shotgun sequence.
ACCESSION   DASCTH010000247 DASCTH010000000
VERSION     DASCTH010000247.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000482279"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /gene="fusA"
                     /locus_tag="QGH35_05285"
     CDS             <1..611
                     /gene="fusA"
                     /locus_tag="QGH35_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961602.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HJL81979.1"
                     /translation="VEQEAKFVRQTGGRGQYGHVYLRIEPQEPGTGYEFVNAIKGGAI
                     PKEFIPAVDKGIQGQMANGVITGFPVVDVKATLYDGSFHDVDSSEVAFRVAGSMAFKE
                     GALKANPVLLEPVMKIEVVTPDDYMGDVNGDLNRRRGILQGLDDSPSGKIIRAEVPLA
                     EMFGYATDLRSMTQGRATFTMEFEKYSEAPKKRAEALSVTGN"
     gene            614..928
                     /gene="rpsJ"
                     /locus_tag="QGH35_05290"
     CDS             614..928
                     /gene="rpsJ"
                     /locus_tag="QGH35_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HJL81980.1"
                     /translation="MADNQSIRIRLKSFDHKLIDTSTEEIVETAKRTGSTVSGPIPLP
                     TSRERFTLLTSPHVNKDARDQYELTTHKRVMDIINPSDKTVDALMKLELPAGVDVQIK
                     LN"
     gene            1164..1796
                     /gene="rplC"
                     /locus_tag="QGH35_05295"
                     /pseudo
     CDS             1164..1796
                     /gene="rplC"
                     /locus_tag="QGH35_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017223584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
BASE COUNT          713 a          306 c          409 g          527 t
ORIGIN      
        1 tggttgaaca ggaagcaaaa tttgttcgtc agacgggtgg tcgaggacaa tatgggcatg
       61 tctatcttag aatagagccc caggagcctg gaacaggtta tgaatttgta aatgcaataa
      121 aaggcggggc catcccaaag gagttcatac ctgctgtgga taaaggtatt caaggccaaa
      181 tggcaaatgg tgtgattacc ggatttcctg tagtagatgt aaaagcaact ctttatgatg
      241 gatcctttca tgatgttgat tcaagtgaag tagcttttag ggttgccggt tcaatggctt
      301 ttaaagaagg tgcattgaag gcaaatcctg tactattaga gcctgtaatg aagattgaag
      361 ttgttacacc tgatgattat atgggtgatg ttaatggaga tttaaatagg agaagaggta
      421 ttctgcaagg tttggatgat tcaccttctg gaaaaattat aagggctgaa gtcccgcttg
      481 ctgaaatgtt tggttatgca actgatttaa gatccatgac acaaggtaga gcaactttta
      541 caatggaatt tgaaaaatat agtgaagcac caaagaaacg agctgaagca ctatcagtaa
      601 caggaaatta attatggcag ataatcaatc aattagaatt agattaaaat cgtttgatca
      661 taaacttata gatacatcaa ccgaggaaat agttgaaact gccaaaagga caggctcaac
      721 ggtatcggga cctataccac ttcccacctc tagggaaaga tttacattgc ttacatcacc
      781 tcatgtgaat aaagatgcca gagatcagta cgagttgacg acacataagc gagtcatgga
      841 tataatcaat ccaagcgata agactgtaga tgctcttatg aagcttgaat tacctgcagg
      901 tgtagatgtt caaataaaat tgaactgata ttaaaaattg atataattcc cgaacgcttt
      961 tagcctgata aatggatgtg atttataggc tatttttgca gggttatggc tgcaatattt
     1021 ggatgattta tttgcttgtt tcttaattta ttttttttaa taaaaaagca taaagaacaa
     1081 caacttaagt ttgagtataa aatctatttt ttgaatggaa attagaagtt taaaaaacat
     1141 taataatttt tttgagaatc aaaatgattg gtatgattgg aaaaaaatgc ggaatgacac
     1201 gagttttttc agacgatgga cagtctgtac ctgttactat tatccaagcg aaccccaatc
     1261 gaataaatcg tattaaatca tcagaaactg atggttacaa agcaattcag gtaacaactg
     1321 gccatgctaa atctaaaata actaagccca ctaagggtca atacgtcaaa aataatatta
     1381 atccaggaac taagcttcat gagtttaaac tcaataatga tgaagcagag caagttgaat
     1441 caggtaaaga gattacagtt agctacttta aaaaaggaga acttgttgat gttgtcggca
     1501 aaacaatagg taagggattt gcaggtgtaa taaaaagaca caattttagt atgcaagatg
     1561 ctacacacgg taattcttta tcacatagag cacctggatc tatcggccaa aatcaaactc
     1621 ctggaagagt atttaaaggt aaaaaaatgg cagggcatat gggtaatgtt agaaaaacaa
     1681 cacagaatct tgaagtagta gaaattgatg aagatagaaa tttactattt attaaaggtt
     1741 ccatagctgg acatgtaaat tctgatgtta ttattatgcc agcgattaaa tctaaacaag
     1801 atgataggag aatattccca gaaacccaag acacccaaga ggccgaagta gaaacccaag
     1861 acacccaaga ggccgaagta gaaacccaag acacccaaga ggccgaagta gaaacccaag
     1921 acacccaaga ggccgaagta gaaacccaag acacc
//
LOCUS       DASCTH010000248         2279 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000493073, whole genome shotgun sequence.
ACCESSION   DASCTH010000248 DASCTH010000000
VERSION     DASCTH010000248.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000493073"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="QGH35_05300"
     CDS             complement(<1..278)
                     /locus_tag="QGH35_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015605.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJL81981.1"
                     /translation="MNESVTTIPIASLLLGFLPVILLIAIMWVWRLNALQAIYANIRM
                     LVQLLLIGYVLTYIFETDEPVLVVLVVIFMIAMSSWIALRPLADRGIKP"
     gene            complement(290..1426)
                     /locus_tag="QGH35_05305"
     CDS             complement(290..1426)
                     /locus_tag="QGH35_05305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020035672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HJL81982.1"
                     /translation="MNQEPKKIQLGEGTISGYLSIFFSLVSFGAVICFLFPEYFTTEE
                     FRSYYPIAILRWVLFACLFFSFMFALLSILLSKRTKNAVIGMSISVLAILLGGTGIEV
                     KEFEQSIFSISLDWMLLEILVLSLIFIPIEMFLPKRSEQTKFHPEWKTDLIYLFKAQL
                     LVQYVAVAVKLPAELFFSDIGMEQVQNLVSYLPFLLQLFLAMFVADLFQYIAHRSFHV
                     NSLLWRFHSVHHSIKYVDWISGSRLHMVDIFVTRSFSYIPLYVLGFSNDVFYVYVVIV
                     AIQAVIAHANTRIPFGPLKYLLVTPQYHHWHHGKDQKFYNTNYAIHFPFIDKMFGTYY
                     LPGNDWPQEMGLKNEKFPKGYFRQFIYPFIRDPKFFEPSDPSTR"
     gene            complement(1410..1976)
                     /locus_tag="QGH35_05310"
     CDS             complement(1410..1976)
                     /locus_tag="QGH35_05310"
                     /inference="COORDINATES: protein motif:HMM:NF021998.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HJL81983.1"
                     /translation="MSKELFVIGVVAFFVGYLLADLLTGTVHWFCDTFFSENAPFIGP
                     IIIASFREHHTHPQLFTKDKFIEQDTTSFFVLLTPLFLAVFSESSYSHDLNSFFWHST
                     LIGLSIGAFGTNLFHKWAHQTNPPLLAQKLQKMGLILTPDRHNKHHRDYRKSFCVTSG
                     WLNFILDYTRFFLILERFFRLFSYEPRT"
     gene            complement(2086..>2279)
                     /locus_tag="QGH35_05315"
     CDS             complement(2086..>2279)
                     /locus_tag="QGH35_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL81984.1"
                     /translation="KTVNLKGIKMPPICMNFVVDETLALSDNQFQSSEYKECRSILLR
                     ELVKEETTKDNQFQIVIKP"
BASE COUNT          811 a          420 c          417 g          631 t
ORIGIN      
        1 ggcttgatac cgcgatctgc caacgggcgt agtgcaatcc atgatgacat agcgatcata
       61 aagatcacca ctaacacaac aagcactggc tcatcagtct caaaaatata ggtgagcaca
      121 tagccaatca gcaaaagctg cacaagcatg cgaatgttgg cgtaaattgc ctgcaacgca
      181 ttcaggcgcc agacccacat gatggcgatc agcaaaatca ccggcagaaa acccagcaac
      241 aggcttgcaa tcggaattgt ggtaacggat tcattcattg tttttaaaac taccttgtgc
      301 ttggatcact gggttcaaaa aattttgggt ctctgatgaa tggatatata aattgtctga
      361 aatagccttt aggaaacttc tcatttttaa gacccatttc ttgcggccag tcattgccag
      421 gaagataata agtaccaaac atcttatcaa taaatggaaa atgaatagca taatttgtgt
      481 tataaaattt ttggtcctta ccatggtgcc aatgatgata ctgaggagtt accaataagt
      541 actttaaggg tccaaatgga atgcgggtat ttgcatgagc gatcaccgcc tgaattgcaa
      601 caattacgac atatacataa aaaacatcgt tagagaaacc aagcacgtat aaaggtatat
      661 aggagaaaga cctggtaaca aaaatatcaa ccatatggag tcttgatcct gaaatccagt
      721 ctacgtattt tatcgagtga tgaactgaat gaaatctcca tagaagacta ttaacatgaa
      781 acgaacggtg agcaatgtat tgaaataaat cagccacaaa cattgcaaga aataattgca
      841 ataagaaggg taagtaagac acaagatttt ggacttgttc cattcctata tcactaaaaa
      901 atagttctgc aggtaactta actgcaaccg caacatactg cactagaagc tgtgccttaa
      961 ataaataaat cagatcggtc ttccactcag gatgaaattt agtctgttca gaacgctttg
     1021 gtagaaacat ctcgattgga ataaaaatta gtgaaagcac taaaatttcc aagagcatcc
     1081 aatccaggct gatggaaaat atactttgct caaattcttt cacctcaatg ccagttcctc
     1141 caagcaagat agccaaaaca gaaatcgaca ttccaataac agcatttttg gttcttttgc
     1201 taagcaagat actcaaaagt gcaaacataa aagagaaaaa taagcaggca aataataccc
     1261 atctcaatat tgctatcggg taataagacc taaattcttc agtagtgaag tattctggaa
     1321 agagaaaaca aatgactgct ccaaatgaaa ccagtgagaa aaatatcgat aaataaccac
     1381 ttatcgtacc ttctcccagt tgtatttttt taggttcttg gttcataaga aaagaggcgg
     1441 aaaaaacgtt ctaatattaa gaagaatctt gtgtaatcaa gtataaaatt caaccatccc
     1501 gaagtcacac aaaaactttt acgatagtct ctgtgatgtt tgttatgtct atcaggagta
     1561 agaattagac ccattttttg taacttctgt gccaacaaag gagggtttgt ttgatgggcc
     1621 catttgtgaa aaaggtttgt tccaaaagct ccgatggata aaccgattaa agtggagtgc
     1681 caaaaaaagg aatttaaatc atgtgaatat gaagattcag aaaaaacagc taaaaacaaa
     1741 ggggttaaaa gaacaaaaaa acttgtcgtg tcttgctcta tgaatttgtc tttggtgaac
     1801 aactgtgggt gagtatgatg ctctctaaaa gatgcaatga ttattggtcc tataaaggga
     1861 gcattctcac taaaaaaagt atcacaaaac caatggacgg ttcccgtaag aagatctgct
     1921 aaaagataac caacaaaaaa agcgaccacc ccaataacaa acagttcttt tgacaacagg
     1981 ttgttgctta tttttacaaa aagtgtaatt agcaacccgg ttgaaacaag gacaactata
     2041 tatttagtga aagttaatac tcttccctgc tcttccatat acgacttatg gtttgattac
     2101 aatctggaac tgattatctt tggtcgtctc ttctttcact aactctcgta ataagattga
     2161 cctacattct ttatattcac tagattgaaa ttggttatct gaaagagcaa gtgtttcgtc
     2221 aacaacaaag ttcatacaga ttggtggcat ttttatccct ttcaagttca cggtttttt
//
LOCUS       DASCTH010000249         1780 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000496377, whole genome shotgun sequence.
ACCESSION   DASCTH010000249 DASCTH010000000
VERSION     DASCTH010000249.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000496377"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="QGH35_05320"
     CDS             complement(<1..1076)
                     /locus_tag="QGH35_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012766.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HJL81985.1"
                     /translation="MNSRDYIIRRKKLAKAIGKGSVAILPAAQTSQRSRDTKYKYRPD
                     SDFYYYTGFSEPESLMILAPGRSEGEFILLVEKKDPLAETWEGHRPGPKGAIKDYGAD
                     QSFSIDQCDAVLSKILKTTEKIFFTLGNDTSYDQKITNILNSLRAGQRQGISVPDSII
                     SLESITHELRSIKSEKEIKLMRKAAKIGVEAHFRVMKKCQENLYEYQLEAEILHQFGQ
                     YGATASYESIVASGSNACTLHYINNRSKLKNGELLLTDAGCEFEMYASDITRTIPISG
                     IFTDEQKAIYSIVLQTQKKAIASVKPGQTFKNLQNDAIKSITKGLQKLGILSGNIDQL
                     IANEAYKPFYMHGIGHWLGMDVHDV"
     gene            complement(1073..1615)
                     /locus_tag="QGH35_05325"
     CDS             complement(1073..1615)
                     /locus_tag="QGH35_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015642.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HJL81986.1"
                     /translation="MEFSEIDLLRRKANVAIDISEYHGTICAYLCCDSFQEDDLLPKE
                     VSFDGSSVSVQIIEFKDALYELISETINSFNDTEMSFYPFLPLDSEELSIRAHALSIW
                     CQGFVDALGMFISERELTINQSDQTFISEIIEDFSQISTLSKTTVENGNEEELAYFEV
                     VEFVKVGTQLVYEELKREIN"
BASE COUNT          523 a          351 c          292 g          614 t
ORIGIN      
        1 acatcatgca cgtccatacc cagccaatga cctataccat gcatataaaa aggtttatat
       61 gcctcattgg cgatcaattg atcgatattg ccactcaaga tacccaattt ctgtaacccc
      121 tttgtgattg atttgattgc atcattttga agattcttaa aagtctgtcc tggctttact
      181 gatgcaattg cttttttttg ggtttgcaag acaattgaat agattgcttt ttgttcatcg
      241 gtaaaaatac ctgaaatggg gatggttctt gtgatatcac tagcatacat ctcaaattca
      301 caaccagcat cggttaatag aagctcccca ttctttaatt tagatctatt atttatatag
      361 tgtagtgtgc atgcattact tcctgaagct acaatacttt cgtatgaagc agtggctccg
      421 tactggccaa attggtgtaa tatttctgct tcaagctggt attcataaag attttcctgg
      481 cattttttca ttactctgaa atgagcttct acgccaattt ttgcagcctt tctcatcaat
      541 tttatttcct tttctgactt gattgatctc aattcatggg taatggattc caatgaaata
      601 atactgtcag gaactgagat tccttgcctt tgtcctgctc ttaatgagtt taatatattg
      661 gtgatctttt gatcatagga ggtatcgtta ccgagagtaa aaaatatttt ttcagttgtt
      721 ttcaatattt tactcagcac tgcatcacac tgatcgatac taaaagattg atcagcccca
      781 taatctttaa tggccccttt gggacccggt ctatgaccct cccatgtttc tgccaatgga
      841 tctttctttt ctacaagtaa aataaattca ccctcagacc ttcctggcgc caatatcatc
      901 aaagattcag gttcgctaaa tcccgtgtaa taataaaaat cactatcggg acgatacttg
      961 tatttagtgt ctctgcttct ctggctggtc tgtgctgcag gaagaatggc tacagagcct
     1021 ttgccaattg cttttgcaag ttttttgcgt cttattatgt agtctctgct attcaattta
     1081 tttctctttt caattcttcg tacaccaatt gggtgcctac tttgacaaat tcaaccactt
     1141 caaagtaagc gagctcttct tcgttcccat tctcaactgt ggttttgctt agtgtagaga
     1201 tttgagaaaa atcttcaatg atttcactta taaatgtttg atcagattga tttattgtca
     1261 attctctttc agagataaac attcccaagg catcaacaaa accctgacac caaattgata
     1321 aagcatgcgc tctgatgctc aactcttctg aatctagagg gagaaaagga taaaaactca
     1381 tttcagtatc attaaaacta tttattgtct ctgaaatcag ttcataaaga gcatccttga
     1441 actcgataat ttggacagaa acagaactgc cgtcaaaact tacttctttg ggtaataaat
     1501 catcttcctg aaaagagtca cagcacaaat aggcacaaat ggttccatgg tattcagata
     1561 tatcaatggc cacatttgct ttacgtctaa gtagatctat ttcactaaat tccatttttt
     1621 ctcttttaaa gtgagctgaa aatgcctttg attgtatcct tatttgacca ctaggtcagg
     1681 aaaatctata gtgaatataa tgagtaaaat aacagaaaaa gacatattga attcacttga
     1741 aaagctgaca acaaggattg aagacttaat tgaaaaatgc
//
LOCUS       DASCTH010000250         2945 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000502586, whole genome shotgun sequence.
ACCESSION   DASCTH010000250 DASCTH010000000
VERSION     DASCTH010000250.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000502586"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="QGH35_05330"
     CDS             complement(<1..435)
                     /locus_tag="QGH35_05330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HJL81987.1"
                     /translation="MVYIKYILIVVVFLHPGHINAELPNQNAIASAHPLATQAGHLIL
                     QRGGNAFDAAVAVSAALSVVEPYSSGLGGGGFFLLHRNSDDFTTFIDGREVAPLMSKP
                     DMYLDSDKNFIPKSSLVGPLASGIPGLPAALVHISKQYGKLPL"
     gene            complement(462..938)
                     /gene="coaD"
                     /locus_tag="QGH35_05335"
     CDS             complement(462..938)
                     /gene="coaD"
                     /locus_tag="QGH35_05335"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010374242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HJL81988.1"
                     /translation="MIAIYAGTFDPITNGHFEMARRASNIFETVIVGISHASEKKTIF
                     SPEERLEMAKEVLNTFNNIEIKEYSGLTIDFAKSEKANVLIRGLRSPFDAQFEFELAN
                     MNKRLAEDIETVFFSSSGSLAFVSSSLIKQIASEGGDVSEFVHPTVQKALQEQFQN"
     gene            complement(954..1517)
                     /gene="rsmD"
                     /locus_tag="QGH35_05340"
     CDS             complement(954..1517)
                     /gene="rsmD"
                     /locus_tag="QGH35_05340"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HJL81989.1"
                     /translation="MSTKGKIRIIGGQHRGRKVSIVDNVIKPTKDRVRETLFNWLAFQ
                     TEGKRCLDLFAGTGILGIEALSRGASDIVFVDHRRKILKEIQKQALYLGLENFRVVHS
                     DAQLFLNDPNCFQPFDLIFLDPPFSGYKIQDFLNHLESCKWLNPGGLIYYESDQKLDC
                     KEYSMSWENYKESKAGNVYYGLLKRKQ"
     gene            1692..2555
                     /gene="rpoH"
                     /locus_tag="QGH35_05345"
     CDS             1692..2555
                     /gene="rpoH"
                     /locus_tag="QGH35_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HJL81990.1"
                     /translation="MAKELTIKQDEIFMGPLGSLGAYINIVNQVPILSKEEEKSLALR
                     YQNENDLDAARRLIMSQLRFVVYIAKGYSGYGLSLADLIQEGNIGLMKAVKRFDPDRG
                     FRLVTYAVHWIRAEIHEFVFNNWKLVKIATTKAQRKLFFNLRKSKKHLGWLSKQETES
                     IAKDLGVKPKEVTQMEQKLTSKDLAFDLRDSSNEDEYVAPAGYLPSPDSDPAETLQES
                     DWLTKNTEALTLAMKNLDERSKNILKSRWLEEKKPTLKDLSKKYNISIERVRQIEEHA
                     IKTLRETLVEA"
     gene            2533..>2945
                     /gene="trmB"
                     /locus_tag="QGH35_05350"
                     /gene_synonym="yggH"
     CDS             2533..>2945
                     /gene="trmB"
                     /locus_tag="QGH35_05350"
                     /gene_synonym="yggH"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.3"
                     /note="tRNA (guanine-N(7)-)-methyltransferase; catalyzes
                     the formation of N(7)-methylguanine at position 46 (m7G46)
                     in tRNA by transferring the methyl residue from
                     S-adenosyl-L-methionine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HJL81991.1"
                     /translation="MKPSSKLNSLRTIKSFVRRSGRITNSQRKAWNHYWSYYGIDIEN
                     DPNEFIELFNDTKKRIVLEIGFGDGDNLIATAINEPSSTVIGIEVYRSGIGHCLINAK
                     KNNLKNLKIIYFDAVEVLNQYVPNNSVDAINIFFP"
BASE COUNT          965 a          530 c          517 g          933 t
ORIGIN      
        1 taatggtagt tttccatatt gtttagaaat atggactaaa gctgctggaa gtccaggtat
       61 tcctgatgct aaagggccaa ctaaagatga cttgggtata aaattcttat ctgagtccag
      121 gtacatgtct ggttttgaca tcagaggtgc aacttctcgt ccatctataa atgtagtgaa
      181 atcgtcgcta ttcctgtgca aaagaaaaaa accacctcct cccaaacctg agctataggg
      241 ttctacaaca gataatgcgg cactgactgc aactgctgca tcaaaagcat taccacccct
      301 ttgcaaaatg aggtgacctg cttgagttgc taaaggatgt gctgatgcaa tggcattttg
      361 atttggaagc tcagcattaa tatgtcctgg atgtagaaaa accacaacaa ttaaaatata
      421 tttaatataa accatatgtt tctttatcgt ggacacagct tttagttttg aaattgttct
      481 tgtagagctt tttgtacagt tggatgtaca aactctgaga catcccctcc ttctgatgca
      541 atttgtttaa taagggacga ggaaacaaaa gccagactgc cgctgctact aaaaaatacc
      601 gtttcaatat cttctgcaag tcttttgttc atatttgcta actcaaattc aaactgagca
      661 tcaaagggtg agcgaagccc tctaattaaa acattagctt tctctgattt agcaaaatca
      721 atggttagcc cagagtactc ttttatctca atattattaa aagtatttag aacctctttt
      781 gccatttcca atctttcttc gggtgagaaa attgttttct tctctgaggc atgtgaaatt
      841 cccacaatca ctgtttcaaa aatattggag gcccttctgg ccatttcaaa gtgaccgttg
      901 gtaataggat caaaggttcc tgcataaata gctatcatga ctgaaatgtc tctttattgt
      961 tttcttttaa gaagaccata atatacattt ccagccttgg attctttata attttcccaa
     1021 ctcattgagt attctttgca atccaatttt tgatcagatt cataataaat taaacctcca
     1081 gggttcagcc atttgcagct ctccaaatgg tttaaaaaat cttgaatctt ataaccacta
     1141 aagggtgggt ctaaaaatat tagatcaaag ggttggaaac aatttggatc atttaaaaac
     1201 aattgtgcat cagaatgaac cactctaaaa ttttctagac ccaaatacag tgcttgtttt
     1261 tgtatctctt ttaatatctt tcttcgatga tcaacaaaga caatatcgct tgcacctctt
     1321 gacaaggctt caattcccaa gataccagtg cctgcaaaaa gatccaaaca tctttttcct
     1381 tctgtttgaa atgccaacca attaaacaat gtttctctca ctctgtcttt tgttggttta
     1441 ataacattgt ctacaattga cacctttctg cctcggtgtt ggccacctat tattctaatt
     1501 tttcccttag tgctcataaa tctttaccgc aaatatttac tttcttgctc aattgtagag
     1561 caattttttg tttggttata tttataattt attgatttat agatattttt taaccaagaa
     1621 tgtattatgt ttagcactct cattgttgga gtgctaaaat tagtgtgtaa actgaacaaa
     1681 aattagaaaa catggctaaa gaattaacga taaagcaaga cgaaatattc atgggtccct
     1741 tgggaagtct tggggcttat ataaatattg tcaatcaagt tcctattcta agtaaagaag
     1801 aggagaagag tctagcgctt cgctatcaaa atgaaaacga tctcgatgct gcgaggcgtt
     1861 taatcatgtc ccagttaaga tttgttgttt atattgcaaa aggttattca ggctatggtc
     1921 tctctcttgc tgatttaatc caagaaggca atataggttt aatgaaagct gtgaaacgtt
     1981 ttgatccaga tcgtggattt agattggtta catatgctgt gcactggata agggcagaga
     2041 ttcatgagtt tgttttcaat aattggaagc tagttaagat cgccacaact aaggcgcaga
     2101 gaaaactttt ctttaaccta agaaaaagca aaaaacattt agggtggttg tccaaacaag
     2161 aaacagaatc tattgcaaaa gatttgggcg tcaaaccaaa agaagtcaca caaatggaac
     2221 aaaagcttac atcgaaagat ttggcttttg atctaagaga tagcagtaat gaagatgaat
     2281 atgtggctcc tgccggttat ctaccaagtc cagattccga tccagcagaa accttgcaag
     2341 aatctgactg gctgacaaaa aataccgagg ctctcactct tgcaatgaaa aacttagatg
     2401 aaagaagtaa aaatattctt aaatcacgtt ggcttgaaga aaaaaaaccc actcttaaag
     2461 acctttcaaa aaaatacaat atctcaatag aaagagtcag gcaaatcgaa gaacatgcta
     2521 tcaagacact tcgtgaaacc ctcgtcgaag cttaactcac tacgtacaat taaaagtttt
     2581 gttcgacgct caggtcgaat aacgaactca caacgcaaag catggaacca ttattggtct
     2641 tattatggta ttgatataga aaacgatcca aatgagttta ttgagttgtt taatgataca
     2701 aaaaaaagaa ttgtcctaga gattggtttc ggcgatggtg ataatctcat tgcaacggca
     2761 ataaatgaac ctagctcaac tgttattggt atcgaagtct atcgttcagg aataggtcat
     2821 tgtttgatta atgcaaagaa aaataaccta aaaaatttaa aaattatcta ctttgatgct
     2881 gttgaggttt taaatcaata cgtaccaaat aattcggttg atgctatcaa tatttttttt
     2941 ccaga
//
LOCUS       DASCTH010000251         2638 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000511795, whole genome shotgun sequence.
ACCESSION   DASCTH010000251 DASCTH010000000
VERSION     DASCTH010000251.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000511795"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="QGH35_05355"
     CDS             complement(<1..473)
                     /locus_tag="QGH35_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL81992.1"
                     /translation="MKTINSKLFFNLAVIGLLLVTFLSVQAQDDQAGSTNIEEITVTG
                     SQIKGAKITGALPVSVLEFTDIDSLGVDGGDELLENVVENGMNLFNETENASGGVNSA
                     RGDMGAYNLRNMGVGNTLTLLNGRRLVNSPGYQTELIGGDYVPTMTVNSNLVPVYG"
     gene            716..2185
                     /locus_tag="QGH35_05360"
     CDS             716..2185
                     /locus_tag="QGH35_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008845126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HJL81993.1"
                     /translation="MKQNSQKNKKNLGFIFGLLGAIIVLLLPNPESLSIEAQRAAAVF
                     VWMGVWWATEAVPIAVTALIPLVCFPLLGVLDIESTAAPYANKNIYLFLGGFFLSIAI
                     QKSNLHKRIALNILKYTGTGGKSIIGGFMLSAGLLSMWIMNTSTTIMLLPIGLAIIAV
                     VKESMGNLSKTDKVNFQVALLLGIAYGANIGGMATLIGTAPNMALNGFMEDNYNVSIS
                     FLDWMKVGLPLSMILLPITWWTLTTVSFPVSFKSSFETQRTIENMRLDLGTIKTPEKR
                     VFIIFVITALLWVFRSPSDSWGINKIPGLEGLTDPGIAMLCGLMLFLTPNGDNTHRNL
                     LEWKDAQEGIPWGILILFGGGLSLAFAAQSTGLAEWLGSLVPGGLNTIVLVFIFTTLV
                     IFLTELTSNLATTATFLPIVAAIALQFDFNPLLLTASVALAASCAFMLPVATPPNAIV
                     FGSELISVPQMMKAGILINLIAIILVSLVGIYLIPAFLI"
     gene            2214..>2638
                     /locus_tag="QGH35_05365"
     CDS             2214..>2638
                     /locus_tag="QGH35_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HJL81994.1"
                     /translation="MKNICVFAGSSVGKRESYSKAAKDLAQAIIENGHRIVYGGGSNG
                     LMGILGDVATEMGGHVTGIITEQLNAVEVGHKRLSELVIVKTMHDRKRIMAERSDSVI
                     CLPGGVGTWEEFFESLTWNQLGLQSKPIVLLNIEDYYSTL"
BASE COUNT          777 a          507 c          539 g          815 t
ORIGIN      
        1 ccgtatacag gaaccaagtt ggagttcact gtcattgttg gaacgtaatc tccaccgatt
       61 aattcagttt gatatcctgg cgagttaacc aatcttcttc cgtttaacaa ggttagagtg
      121 ttgccaacac ccatatttct cagattgtag gcacccatat cacctctggc tgagttcact
      181 cctccagagg cattctcagt ttcattaaat agattcatgc cattttcaac cacgttctca
      241 agcagttcgt ctccgccatc cactccaaga ctatctatat ccgtgaattc caaaaccgat
      301 actggtaatg caccagtaat tttggcgcct ttgatctgac taccggttac agtaatctct
      361 tctatattag tagagcccgc ttgatcatct tgtgcttgca cagaaagaaa agtaactaaa
      421 agtagaccta taactgctaa attaaaaaac agtttagaat taatagtctt catttattca
      481 tcccccaaat aaaaaatatt agataattat catataacta tatctgatta atcagggcat
      541 tatcttattt ctggtgcttc aaaacaagta aaatgagaat gattattaaa tagttctcat
      601 aaagtaatgg aggatttagg atagactttg tccagtgggt ctggatatac tcaatcaaat
      661 tccatgatga ccatagcgat taagggaaat acttctaaat tattattaat aaggaatgaa
      721 acagaacagt caaaaaaata aaaaaaacct aggctttatt tttggtcttt taggggcaat
      781 tattgtgctc ttgttaccca atcctgaatc tttatccatt gaagcccaaa gagcggctgc
      841 agtatttgtc tggatgggag tttggtgggc taccgaggca gtacccattg ctgtgaccgc
      901 actaatacct ttggtttgtt ttcccttact gggtgtccta gacatagaat caacagctgc
      961 tccttatgca aataaaaata tttacctttt cttgggaggt tttttcttat cgatagcgat
     1021 acaaaaatca aacttacaca aaagaattgc attaaatatt ctcaagtaca ccgggacagg
     1081 tggtaaatca atcatcggtg gttttatgtt aagtgccggt ttactgagca tgtggatcat
     1141 gaatacctct actaccatca tgcttctacc aataggcttg gcaatcattg cggttgttaa
     1201 agaatcaatg ggtaatctaa gtaaaacaga caaagtcaat ttccaagtgg cccttttgct
     1261 tgggatcgct tatggggcca atattggagg tatggcaact ttgattggaa cagcacctaa
     1321 tatggcatta aatgggttca tggaagacaa ttataatgtc tctatttcgt ttttagactg
     1381 gatgaaagtt gggctcccct tgagtatgat actgctccca ataacttggt ggactttgac
     1441 cacagtgagc tttccagtga gctttaagtc ctcatttgaa acccagagaa ccattgagaa
     1501 tatgagacta gatttaggaa ccataaaaac acccgagaaa agagtgttta ttatttttgt
     1561 tatcacagca ctcctttggg tttttagatc accttcagac agctggggca ttaataaaat
     1621 tcctggatta gaaggcctca ctgaccctgg aattgctatg ttgtgtggtt taatgctgtt
     1681 cctaacacca aatggtgaca acacacacag aaaccttctg gagtggaaag atgcccaaga
     1741 aggaatccct tggggaatct tgatactctt tggaggaggt cttagtttag cttttgcagc
     1801 acaaagcaca ggattagcgg aatggttggg tagtttagtg ccagggggat taaacaccat
     1861 tgtgctagtt tttattttta caacccttgt tatttttctc acagaattaa cttcgaatct
     1921 tgcaaccaca gcaacttttc ttccaatagt ggcagcgatt gcactgcaat ttgatttcaa
     1981 cccgttgtta ctgaccgcct ctgttgcatt ggcagcaagt tgtgctttta tgttgccagt
     2041 agccactcca cccaatgcca ttgtgtttgg atcagaactg atttcggttc ctcaaatgat
     2101 gaaagcgggt attttgatca atctgattgc catcattcta gtgtcattgg ttgggatcta
     2161 tcttatacct gcttttttaa tctagtgtct tgcgaggata gtaagttaag gctatgaaaa
     2221 atatttgtgt atttgcagga tctagtgttg gcaaacgaga aagctacagc aaagcagcca
     2281 aagatcttgc ccaagctatc attgaaaatg gtcatcgtat cgtttatggc ggtggtagta
     2341 acggactcat gggaatttta ggtgatgtcg ccactgaaat gggtgggcat gttaccggaa
     2401 ttattacaga acagctcaat gctgtggaag tgggtcataa aaggttaagc gaattagtca
     2461 tagtcaaaac catgcacgat cgaaaaagaa taatggccga acgatctgat tcggttattt
     2521 gcctacccgg tggtgttgga acatgggaag aattttttga gtcactgaca tggaaccaac
     2581 tgggtttgca atcgaaaccg attgtactgc tcaacataga agactattat tcaacact
//
LOCUS       DASCTH010000252         2390 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000520787, whole genome shotgun sequence.
ACCESSION   DASCTH010000252 DASCTH010000000
VERSION     DASCTH010000252.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000520787"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /gene="hisE"
                     /locus_tag="QGH35_05370"
     CDS             complement(<1..257)
                     /gene="hisE"
                     /locus_tag="QGH35_05370"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HJL81995.1"
                     /translation="MIEKQNEFIFELEGIIQQRLTDRPEDSYTVGLVNSGIGQVAKKL
                     VEESSELAIASVEQKDSKEISHEAADLIYHFLILLNTVGLS"
     gene            complement(254..1021)
                     /gene="hisF"
                     /locus_tag="QGH35_05375"
     CDS             complement(254..1021)
                     /gene="hisF"
                     /locus_tag="QGH35_05375"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011770000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HJL81996.1"
                     /translation="MLTRRIIPCLDVKDGLVVKGVRFRNHQVVGDILNLAVRYNSEGA
                     DELVFYDITASPDERSVDRRWVNRIAANINIPFCVAGGIKTIADAEEILNYGADKISV
                     NSPALLDPDLINHLVGRFGSQCVVVGIDSFQDQKAFKVFRYTGDPSKTVDTAISTLDW
                     VKEVQDRGAGEIVLNCMNQDGVREGYDLEQLTRVREICQVPLIASGGAGIMQHFAEVI
                     KAADVSGTLAASVFHNEQININDLKQYLLKQGIEVRL"
     gene            complement(1003..1740)
                     /gene="hisA"
                     /locus_tag="QGH35_05380"
     CDS             complement(1003..1740)
                     /gene="hisA"
                     /locus_tag="QGH35_05380"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HJL81997.1"
                     /translation="MKLIPAIDLIDGRCVRLHQGKFNEVTYYEPRPVELAKQYQATGF
                     TNLHIVDLDGTKDGYSENTDVIQEIVETSGLQVQLGGGIRSSDDIVKWLDIGVFQTIL
                     GSYAILNIDRLEDAFKDVELSRIIIALDILYQSEEPIVMSHGWQQSSGQTLWQLLKIF
                     KDSGFQNFLITDISKDGTLSGPNMNLYRQCVDYSESLRFIASGGLSSMSDIIELASIG
                     LYSAVTGKALLDNKIKTQEVEQFLQEE"
     gene            complement(1744..2346)
                     /gene="hisH"
                     /locus_tag="QGH35_05385"
     CDS             complement(1744..2346)
                     /gene="hisH"
                     /locus_tag="QGH35_05385"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HJL81998.1"
                     /translation="MNSKKKVAIVDCGGANLSSLTFAIERLGLEYKVTNNKDDISNAS
                     HVVLPGVGAAKDAMQRLSQTDLTIAIKDLKQPILGICLGMQLLFDSTMEEDVNCLGII
                     QGECKRFAINPSYPVPHMGWNSIKINKKTPLVDNTIEDAYFYFIHSYYIPLLDQTISS
                     TEYDIEFSSVIQQENFYGVQFHPEKSGAAGSKLLDSFFNL"
BASE COUNT          733 a          522 c          363 g          772 t
ORIGIN      
        1 atactaagcc ctacagtatt aagtaatatt aaaaagtgat agataagatc agcagcttcg
       61 tgtgaaattt ctttggagtc tttttgttct accgatgcaa tagccagttc tgaactctct
      121 tcgactaatt tttttgcaac ctgtccaatg ccagaattca ccagaccaac ggtataactg
      181 tcttcaggtc tatctgtcaa tctttgttga ataataccct ctaactcaaa gataaattca
      241 ttttgttttt caatcatagt ctcacctcta tcccttgttt taatagatat tgttttaaat
      301 catttatgtt tatttgttca ttatggaata cactcgctgc cagagtacca ctgacatcag
      361 ctgctttaat cacttcagca aaatgctgca tgattccagc tccaccagag gcaatcaaag
      421 gaacctggca tatttctctt actctggtta attgctccaa atcataacct tctctgactc
      481 catcttgatt catacaattc agtacaattt caccagcacc tctatcttga acttctttga
      541 cccaatcaag tgtactaatt gcagtatcaa ctgtcttcga tggatcaccc gtataacgaa
      601 ataccttaaa agccttctgg tcttgaaaac tatcaatacc aacaacaaca cactgtgaac
      661 caaatctacc taccagatga ttaatcaaat ctggatctaa caaagcaggg gagttgactg
      721 aaattttatc tgccccataa tttaatattt cttccgcatc agcgattgtc ttgataccac
      781 cggcaacaca aaaaggaata ttgatgttag ctgcaatcct gtttacccat cttctatcta
      841 cagatctctc gtcaggactg gcggtaatat cgtaaaaaac aagttcatcc gcaccttcag
      901 aattatatct gactgccaag tttaaaatat caccaacaac ttggtggtta cgaaagcgta
      961 cacctttaac cactaaacca tcttttacat ccagacaagg aattattctt cttgtaagaa
     1021 ttgttccacc tcctgtgttt tgattttatt gtctaatagt gctttcccag tcactgctga
     1081 gtacagacca attgatgcaa gctctataat atcactcatc gatgacagtc ctccggaagc
     1141 gatgaatctc aaagactctg agtaatcgac acactgacga tataaattca tattgggacc
     1201 tgataatgtg ccatctttgc tgatgtcagt aatcagaaaa ttctgaaagc cgctgtcctt
     1261 aaaaatcttt aataattgcc ataatgtttg accactggat tgctgccagc catgtgacat
     1321 cactattggt tcttcacttt gatataaaat atcgagagct atgataattc tggacaattc
     1381 aacatcttta aaagcatcct ctagcctatc aatatttaaa atcgcgtaac tacccaatat
     1441 cgtctgaaac accccaatat ctagccattt tacaatgtca tctgaagatc ttatcccacc
     1501 acctaattga acctgcaaac ctgatgtttc tacaatttct tgaatcacat cagtattttc
     1561 agaataacca tccttagtac catcaagatc aacaatgtgt aggttagtaa atccagttgc
     1621 ttggtattgt tttgcgagct ctactggtct tggttcatag taagtgactt cattaaactt
     1681 tccttgatgt aacctcacac aacgtccatc aattaaatca attgcaggta ttaatttcat
     1741 agactataaa ttgaaaaatg aatccagtaa cttagagcct gctgctccag atttttcagg
     1801 atgaaattga actccataga aattttcctg ctgaataacg ctggagaatt ctatatcgta
     1861 ttcggtgcta gaaatggtct gatccaataa gggaatgtaa taactatgaa tgaaataaaa
     1921 ataagcatct tcaatagtgt tatctactaa gggtgttttt ttatttattt ttatactatt
     1981 ccatcccata tgaggcaccg gatagcttgg attaatagca aagcgcttac actcaccttg
     2041 gataatcccc aaacaattta catcctcttc cattgtggaa tcaaacaata actgcattcc
     2101 caaacaaatt cccaatatgg gttgtttgag atcttttatg gctatagtca aatctgtttg
     2161 ggaaagtctt tgcatggcat ctttggccgc tccaacaccg ggaagaacaa cgtgtgatgc
     2221 attggatatg tcatctttgt tgttggttac cttatattcg agtcctaatc tttctatggc
     2281 aaaagtcagt gaggataaat tagcaccacc acaatcaact atggcaactt tttttttact
     2341 gttcacaata cacctttgct tgaaggcact gtatcacccg aaatcttaat
//
LOCUS       DASCTH010000253         1571 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000527459, whole genome shotgun sequence.
ACCESSION   DASCTH010000253 DASCTH010000000
VERSION     DASCTH010000253.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000527459"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="QGH35_05390"
     CDS             <1..866
                     /locus_tag="QGH35_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004862990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJL81999.1"
                     /translation="AGLPKGLFNVLTGDAKTIGKTMTDSHLVRKLTFTGSTEIGKMLL
                     KECANTVKKVSMELGGHAPFIVFEDADIPAAIEGAIAAKFRNSGQTCVCANRIYIHQN
                     VYDEFCAGFVKEAGQLKTGDGLDPDTDQGPLIDKTALLTVEEHVVDAKNQGGEILLGG
                     KSHQLGGLFYEPTVIKNAHDGMLVAKEETFGPVAPLFSFATETEVIERANDTLFGLAS
                     YLYTQNLAKSWRVSEQLEYGMVGLNTGIISTEMAPFGGIKESGMGREGSKYGIDDYLE
                     TKYISIAGIDE"
     gene            930..>1571
                     /locus_tag="QGH35_05395"
     CDS             930..>1571
                     /locus_tag="QGH35_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013703.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HJL82000.1"
                     /translation="MQTEQAKLTKGPVSSAITSMMVPMIIGLIVIIGNGLVDAYFVGQ
                     LGYAQLAAISYAFPVWFILGGIVMGLGVGTSSVVSRLIGSGNHAQVKEVATHSMILGV
                     CVGIAVISIGLITLEDLFGLLGANEETMPYVKDYMEIYYLGGIFIAVPMIGNSVLRAS
                     GDAKTPSTLMASTAIINAILDPILIFGWFGMPAMGIKGAALASVCANIFVFLAA"
BASE COUNT          456 a          278 c          362 g          475 t
ORIGIN      
        1 aagcaggttt acctaaaggt ttattcaacg tgctgactgg tgatgcaaag actattggaa
       61 aaacaatgac tgacagtcat ttagtgcgaa agttaacatt cactggatcc actgagatag
      121 gaaaaatgct gctaaaagaa tgtgctaaca ccgtcaaaaa agtctctatg gagcttggcg
      181 ggcatgctcc atttatcgtt tttgaagatg cagatattcc agctgctatt gaaggggcaa
      241 ttgcagcaaa gtttagaaac tctggccaga cttgtgtttg tgctaataga atatacattc
      301 atcagaatgt atatgatgag ttttgtgctg gatttgtaaa ggaagctgga caacttaaaa
      361 caggtgatgg cctagaccca gatacagatc aaggaccact tattgataaa actgcattgc
      421 ttacagttga agaacatgtc gttgatgcta aaaaccaagg gggagagatt ctcttaggcg
      481 gaaaatcaca tcagttaggt ggtttgtttt atgaaccaac agtgattaaa aatgcccatg
      541 acgggatgct agtagctaaa gaagaaacct ttggtcctgt agcgccctta ttttcttttg
      601 ccacggaaac agaggtaatc gaaagagcaa atgatactct tttcggtctt gcgtcatatc
      661 tttataccca aaatctagca aaaagttgga gggtttcaga gcagcttgaa tatgggatgg
      721 tgggtttaaa cacaggaatc atttcaactg aaatggctcc tttcggaggt atcaaggaat
      781 ccggcatggg tcgcgaagga tccaagtatg gaatagacga ctatcttgaa acaaaataca
      841 tctctatcgc tggaatcgat gaatagccgc taacgattgc aatctgttgt tacaactgta
      901 tgattcggct attgttttca taaacctcca tgcaaactga acaagctaaa ctaactaaag
      961 gccctgtttc ttcggctata acatccatga tggtaccaat gatcattggt ctcattgtga
     1021 ttattggcaa tggtttggtt gatgcttatt ttgttgggca gcttggctat gcgcaattag
     1081 ctgcaataag ttatgctttt cctgtttggt ttatccttgg cggtattgtt atgggcctgg
     1141 gtgtaggaac ctcatctgta gtatcaagat taattgggtc tggcaatcac gcccaagtca
     1201 aagaagttgc aactcactca atgattttgg gtgtctgcgt gggtatagcg gttatatcca
     1261 taggcctgat tactctggag gatctttttg gtcttcttgg tgcgaatgaa gaaactatgc
     1321 cttatgtaaa agactatatg gaaatttatt atctgggagg tatttttata gccgtgccca
     1381 tgataggaaa ctcagtctta agagcatctg gcgatgcaaa aactccctca acactcatgg
     1441 catcaacagc tatcataaat gctattttag atcctatatt aatttttggg tggtttggca
     1501 tgcctgcaat gggtatcaaa ggggcggctc tagcaagtgt ttgtgcaaat atttttgttt
     1561 ttctagcagc a
//
LOCUS       DASCTH010000254         4326 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000529035, whole genome shotgun sequence.
ACCESSION   DASCTH010000254 DASCTH010000000
VERSION     DASCTH010000254.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000529035"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="QGH35_05400"
     CDS             <1..306
                     /locus_tag="QGH35_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82001.1"
                     /translation="IYDVTSEGIHLETRDPQEFGIPKCNNTDLLGGDANHNAKAVLDV
                     FNNRDQGPHREALALGTALALELTGAVDNMHQGIIEARDTIHNGTAAEFINNLMLLE"
     gene            309..1139
                     /locus_tag="QGH35_05405"
     CDS             309..1139
                     /locus_tag="QGH35_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HJL82002.1"
                     /translation="MTQNFLTSMAKTSQQRFQDASNRLTERELLSSCENMDALEPINL
                     PTDDFHIIAEIKKQSPSSGNLALKNFDLQKQAMSYIEGGSSLLSVLTEPSKFLGELED
                     LELVASLENNIPVMRKDFLVHPYQISEARYYGAAGVLIIVAILDERALQAMIQRALDH
                     GMFVLLETFTKQELRLATNILNRFADNASQLLIGVNCRDLNTLQVDFSNFTKLAADLP
                     HTAMCVAESGVYDLMDLQRIIEMGFGSVLIGSALMQSNNPRTTLNEMKAFARKTIAVK
                     "
     gene            1139..1756
                     /locus_tag="QGH35_05410"
     CDS             1139..1756
                     /locus_tag="QGH35_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012900.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HJL82003.1"
                     /translation="MKTLIKICGLTNQESVDVAVQSGADLIGFVFAKKSPRCLTPALA
                     SQLSGSIPAQIKTTAVMLHPSDAEAREVFSKFLPDYLQTDAKDFISLHLPEGCRPLKV
                     YRDLPQLHFDEMNPNELSLFEGQNSGAGELVNTDHAAAAACHAPIILAGGLNSGNVVG
                     MLQAVQPKGVDVSSGVESELGVKDVQKIKDFINIVRDYDAMKQNA"
     gene            1753..2985
                     /gene="trpB"
                     /locus_tag="QGH35_05415"
     CDS             1753..2985
                     /gene="trpB"
                     /locus_tag="QGH35_05415"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HJL82004.1"
                     /translation="MNNTEKILLNKLLNGEFPDQNGRFGSFGGQFAPETLMPALKKLE
                     KGVKQLLNDNSFQDELTRELQQWAGRPTGLTFAGKLSEAWGVHVYIKREDLAHTGAHK
                     INNALGQALLAKRLGAKRVVAETGAGQHGVATAAACARVGIPCVVYMGSVDIERQSPN
                     VDRMLQLGAEVIPVDSGDKTLRAAIDEAFRDWVAHPDNTYYIIGSVVGPHPYPLLVRE
                     LQSVIGKEAREQIIGATGNLPNSVFACVGGGSNAIGIFHAFLADTEVELVGVEAGGTS
                     DALGENAATLASGKPGILHGSYSMLLHDADGLVQETHSISAGLDYPGVGPEHAFLQSI
                     GRVTYISATDDEALGAVRECCSAEGILPALESAHALVGARKWAAENPGKTILIGLSGR
                     GDKDMPTLSKMHKDLFEE"
     gene            2991..3761
                     /gene="trpA"
                     /locus_tag="QGH35_05420"
     CDS             2991..3761
                     /gene="trpA"
                     /locus_tag="QGH35_05420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HJL82005.1"
                     /translation="MFGSEFIEQSILSAATKGTAIIPFITAGYPEKNEFSNLVLSLSK
                     AADVIEIGVPFSDPMADGVTIQRSSHQAIESGVRLQWILQQLQAIEINKPVILMSYLN
                     PLFVFGYEALVHQSLKAGVDGFIVPDLPIEEGSDFRDMLNEANLGLIQLVTPASPTER
                     IAKVAAMSSGFLYAVTVKGVTGGADGLSNTVTGYLEQVRQLATIPVCAGFGVRTAKDV
                     ALLEPYVDGVIVGSALLEAIESGQQPVEFLNNLRTKEK"
     gene            complement(3820..>4326)
                     /locus_tag="QGH35_05425"
     CDS             complement(3820..>4326)
                     /locus_tag="QGH35_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008490717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJL82006.1"
                     /translation="GHNEKGKSVVIIDSPPPQTLGREEGGLFEIWNTDGDPIDTKDPT
                     DRVEPKVTLSPSAGGTKFRYFAIAPTPEGATREQLEAGMAAAFKQMGAAHDQPDTTRH
                     PGMHKTKTIDYIILLSGNAKLLLDDDEVQLQPFDVVVQRGTNHAWVVDGTESALFIAV
                     LIDSKIVE"
BASE COUNT         1193 a          886 c         1052 g         1195 t
ORIGIN      
        1 atttacgatg tcacctctga aggcattcac ttagaaacaa gagatcctca agagtttgga
       61 attccaaaat gcaacaatac ggatttattg ggaggtgatg ccaatcataa tgcaaaagct
      121 gtattagatg ttttcaataa tagagaccag ggcccccata gggaagcatt agcactgggc
      181 actgccttgg ctttagaact aacaggagca gtggataata tgcaccaagg gatcatcgaa
      241 gcaagagaca ccattcacaa tggaaccgct gctgagttta taaacaattt aatgttattg
      301 gaataataat gactcagaat tttttaacca gtatggccaa aacaagccaa caacgctttc
      361 aagatgcatc caatcgtttg actgagagag agctgctatc ttcttgcgaa aacatggacg
      421 ccctcgaacc aatcaatttg ccaacagatg attttcatat cattgctgaa atcaaaaaac
      481 aatcaccttc atcaggcaac ctcgctttga agaactttga cttacaaaaa caggcaatga
      541 gttacattga aggtggttca agcttgctct cagttttaac tgagccttcc aaatttttgg
      601 gagaactgga ggacttagaa ttagttgcaa gcttagagaa taatattcca gtgatgagaa
      661 aagatttttt agtgcatcct tatcagatat cggaagctag gtattatggc gcagcaggag
      721 ttttgatcat tgtagcaatt cttgatgaga gagcgctcca agctatgatc caaagagctc
      781 ttgatcatgg catgtttgtt ttgcttgaaa catttaccaa acaggaactc cgtcttgcca
      841 ctaacatact aaatagattt gctgacaatg ccagtcagtt attgattggc gttaattgca
      901 gggacctcaa tacattgcag gttgatttct ctaattttac caagttggca gccgatttac
      961 ctcacacagc aatgtgcgtt gcagagagtg gagtgtatga cttgatggat cttcagcgta
     1021 ttattgaaat gggttttggc tcggtattga ttggctcggc cttgatgcaa tcaaacaacc
     1081 ctagaacaac actcaatgag atgaaagcat ttgctcgcaa aacaatagct gttaaataat
     1141 gaaaacattg atcaaaattt gtggcttaac taatcaagaa tctgtggatg ttgctgtgca
     1201 atcaggtgct gatctcatcg gttttgtttt tgcaaagaaa tcacctagat gccttacccc
     1261 tgcattagca agccagcttt ctggctctat tcccgcacag ataaaaacca ctgcggtgat
     1321 gttgcatcca agcgatgcag aagcacgaga agtttttagc aagtttctac ccgattattt
     1381 acaaactgat gctaaggatt ttatatctct acatttaccc gaaggatgtc gtcccctgaa
     1441 agtctaccgt gatcttccac aactccattt cgatgaaatg aatcctaatg agttgtcttt
     1501 atttgagggg cagaacagtg gggctggaga actggtcaat accgatcatg cagcggcagc
     1561 agcctgccac gcacccatta ttttggccgg tggattaaac tctggcaatg tagtgggtat
     1621 gttacaggcg gttcaaccta aaggcgttga tgtgagttcc ggtgtagaaa gtgagttggg
     1681 cgttaaagac gttcaaaaaa tcaaggattt tattaatatt gtcagagact atgatgcaat
     1741 gaaacagaac gcatgaataa cacagaaaaa atattactga ataaattatt gaatggggaa
     1801 tttccagacc agaacggccg atttggttca tttgggggtc aatttgcccc agaaacactg
     1861 atgccggcct tgaaaaaact tgagaaaggc gtcaaacagt tattaaacga taactctttt
     1921 caagatgagt taactcgaga actacagcaa tgggcaggca gacccactgg tctcacgttc
     1981 gctggaaaac tcagtgaggc ttggggagtt catgtttata ttaaacgcga agacctagct
     2041 catacggggg ctcataaaat caataacgca ctgggccagg cgttgttggc aaagcgtctc
     2101 ggtgcaaaaa gagtggttgc agagaccgga gctggtcaac acggtgttgc cacagcagct
     2161 gcttgtgctc gtgttggcat tccctgtgtg gtttacatgg gaagtgtgga tatagagcga
     2221 caatcaccca atgttgatcg gatgctacaa cttggagctg aagttattcc tgttgattcg
     2281 ggcgataaaa cacttcgtgc tgccattgat gaggcttttc gcgattgggt agcacatccg
     2341 gataatacct attacattat tggctcagtt gtcggtccgc atccataccc actattggtc
     2401 agggaactgc aatcggtcat tggcaaagaa gctagggaac aaatcattgg ggctacagga
     2461 aaccttccga attcagtgtt cgcctgtgtt ggagggggtt ctaatgccat tggtatcttt
     2521 catgcttttt tggctgatac agaggtggaa ttggttggcg ttgaagcagg gggaacaagt
     2581 gatgccttgg gtgaaaatgc agcaacactg gcttcaggca agccaggaat tttgcatggg
     2641 agttactcga tgttactgca cgatgcagac gggttggtgc aggagactca ttccatttca
     2701 gcagggctgg attaccctgg cgtcggaccg gagcatgcat tcttgcagtc cattggcaga
     2761 gtaacttata tttctgcaac agatgatgaa gcacttgggg cggttcgcga atgttgttcc
     2821 gcggagggta tcttgcccgc tttggagagt gcccacgcac ttgtcggagc tcggaaatgg
     2881 gcggctgaga atccgggtaa aacaattctg attggattgt caggaagggg tgataaagac
     2941 atgccaactt taagcaagat gcacaaagat ctttttgagg aataaacttt atgtttggca
     3001 gtgaatttat cgaacaaagc attttgagcg ctgctacaaa gggaacagca attattccct
     3061 ttatcactgc tggctatccg gagaaaaatg aattctccaa cctggttttg tcactgtcca
     3121 aagctgctga tgttattgaa ataggggtgc cgttttccga cccaatggca gatggcgtca
     3181 ccatccaaag atccagccat caggctattg agagtggtgt tcgtttgcag tggattttgc
     3241 agcagttaca agcgattgaa atcaataaac cggttatttt aatgagttac ctgaatccgt
     3301 tgtttgtttt cggctacgaa gcattggttc atcagtctct taaagcaggt gtggatggtt
     3361 ttatcgtccc agatttacct attgaggaag gaagtgattt tagagacatg ctgaatgagg
     3421 ccaatctggg tttgattcaa ttggtgacac cggcatctcc cacggagcga attgccaagg
     3481 tggctgccat gagctctgga tttctttatg cggtgactgt caaaggtgtc actggtggcg
     3541 ctgatggttt gtctaatacg gttactggtt atctggagca agttcggcag ctggcaacga
     3601 taccggtttg tgctggcttc ggcgtaagaa ctgcaaaaga tgttgcctta ttggagccct
     3661 atgtggatgg ggtcattgtt ggctctgcat tgctcgaagc cattgaatcc ggacaacaac
     3721 cggttgaatt tttaaacaac cttagaacca aagagaagta atatgcttac cagtctttta
     3781 taatgccaag aggcagcgga tcaataagat aataagctgc tactcgacaa tcttcgaatc
     3841 gatcagcacg gcaataaaca gtgccgattc tgtaccgtcc accacccagg catgattggt
     3901 gccacgctga accaccacat caaacggttg tagttgcact tcatcgtcat caagaagcag
     3961 tttggcattg ccagacaaga gaataatata gtcgatggtt ttggttttgt gcatacccgg
     4021 gtggcgtgtt gtgtcaggtt ggtcgtgggc tgcacccatt tgtttaaaag ctgccgccat
     4081 cccggcttct aactgttcac gcgttgcccc ctcaggtgtc ggtgctatcg caaagtacct
     4141 aaacttggta ccacctgctg acggtgacaa ggtcactttg ggctctaccc ggtctgtggg
     4201 atccttagta tcaataggat ccccgtctgt attccaaatt tcaaacaaac cgccttcttc
     4261 acggccaagt gtttgtggag gcgggctatc aatgataact accgacttgc ctttttcatt
     4321 gtgacc
//
LOCUS       DASCTH010000255         4104 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000530505, whole genome shotgun sequence.
ACCESSION   DASCTH010000255 DASCTH010000000
VERSION     DASCTH010000255.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000530505"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="QGH35_05430"
     CDS             <1..351
                     /locus_tag="QGH35_05430"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HJL82007.1"
                     /translation="IRRFLHYYKFDLSHLCVIHDDLDLQYGSSKIKFDGGHGGHNGLR
                     NVIDVCGSAFLRIRIGIGHPERQDATDYVLSKPTLEEAVSINSALNTALESVDIMLHQ
                     SVAKAMDLLNQSHS"
     gene            complement(354..1376)
                     /locus_tag="QGH35_05435"
     CDS             complement(354..1376)
                     /locus_tag="QGH35_05435"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HJL82008.1"
                     /translation="MTRKLLATTAGSLPKPNWLAEPETLWPKWKLADEQLWKGQQKSA
                     LEWILEQEKAGLEIISEGEQFRIHFVHGFLQKILGISWDKKIKMGIRNDRYTVEVPTV
                     TEAIRKIEKIHSKEAQFLRENTNKAIKFTLPGPMTICDTIANDHYNSRPEMAFEFAKV
                     LNREAKELEAIGVDVIQFDEPAFNSFNQETVEWGIDALEKAAADLKCKTAVHVCYGYG
                     IQENLDWKKTLGDHWVEYKNIFPALNKSSLNQISLEFAGSNVPPELMLLLPDKEIMVG
                     VIGVVTDKIESPEEVKHNILSALKYVERDRLIACSNCGMAPLPKQVAIQKIKALGRGT
                     QLANHT"
     gene            1477..2577
                     /gene="ychF"
                     /locus_tag="QGH35_05440"
     CDS             1477..2577
                     /gene="ychF"
                     /locus_tag="QGH35_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005374148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HJL82009.1"
                     /translation="MGIRCGIIGLPNVGKSTIFNALTRSEVEVANYPFCTINPNIGSV
                     AVPDKRLSVFADMTHIEKVIPSFVEFVDIAGLVAGASKGEGLGNQFLGNIRETDAIVH
                     VVRCFENNDIAHVSENIDPVNDIEIINTELLLADIQTVDKHYVRAEKQSKAAQQGDLE
                     KRDFLKKLLEHLNKFLPARNFILDDEKTDFWMKELHLLSAKPTLYVANINEENVHSGN
                     DFIVQVQEYLGHHQDFELITICGVLESELSQIEGEEQIEFLKDLELEEPALERLIQSC
                     YTLLGLQTFFTTRPGEVRSWPVKVKTKAPQAAGLIHSDFERGFISAEVIPYRDYVELG
                     GEQACKTAGKLRLEGKDYIVGEGDVIHFRFNV"
     gene            complement(2590..3795)
                     /locus_tag="QGH35_05445"
     CDS             complement(2590..3795)
                     /locus_tag="QGH35_05445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007231449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HJL82010.1"
                     /translation="MRKKLICYCGLVLLYASSSILFADTLEYPTDQWTTKTPSTLNVD
                     QKKIDTLFDLSFQDPATQAVVLIKNGFLIGEKYAADFDASSFGTSWSAAKSLYAALIG
                     ISIDQGEINSLDDKVSDYLEYFNDARSEIKIRDLLNMSSGLQFPEHEHELMFFETDHL
                     AYAKDVGVEKQPNKIFEYNNANSMLLGDILLKATGRKADVLLKQRILDPIGVGDHTLW
                     RDAVGNVLSYCCIDMSARDFSRFGLLFSRNGQWNEQTIVSKEFVDETFTVYWDTTPGG
                     ITSQIQRGYNLHWWMSKNDDEGIIFNASGKFGQYIFVDRKNDVIFTRITKYHPTPGSV
                     QDWGTLGGHNVRNISRWIKITRFLNNLGLIDLTEDINTPITQADGESKEFYENYETII
                     DAIAGLSRE"
     gene            3884..3960
                     /locus_tag="QGH35_05450"
     tRNA            3884..3960
                     /locus_tag="QGH35_05450"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3918..3920,aa:Met,seq:cat)
BASE COUNT         1214 a          767 c          761 g         1362 t
ORIGIN      
        1 attaggcgtt ttttacatta ctataaattt gacctttctc acctttgcgt tatacacgat
       61 gatcttgatc ttcaatatgg ttcatcaaaa attaaatttg atgggggaca tggtggtcat
      121 aatggtttga gaaatgtcat cgatgtatgt ggttctgcct ttctgaggat tagaataggt
      181 attggtcatc cagaaaggca agatgcaacc gattatgttt tatcaaaacc cactctagaa
      241 gaagccgtaa gtatcaattc cgcgttaaat actgccctag aatctgtcga tataatgtta
      301 catcaaagcg ttgcaaaggc tatggattta ttgaatcaaa gtcattcttg aactcatgtg
      361 tgattagcca attgtgttcc tcgacctaaa gcttttattt tttgtatagc aacttgctta
      421 ggcaaaggag ccataccaca attactacaa gctattaagc gatcacgttc aacgtatttg
      481 agtgccgata aaatattatg ttttacctct tctggtgatt ctattttatc tgtaactacc
      541 ccaatcactc caaccatgat ctctttgtct ggaagcaata acatcaattc gggagggaca
      601 ttggagcctg caaattctaa tgatatttgg ttaagactgc ttttattaag agcaggaaag
      661 atatttttat attcaaccca atggtctccc aatgtttttt tccagtctaa attttcctgg
      721 ataccgtaac catagcaaac atggaccgct gttttacatt taaggtctgc tgctgccttt
      781 tctaaagcat caattcccca ctcaactgtt tcttggttaa atgaattaaa tgctggctca
      841 tcaaactgaa taacgtcaac gccaatagct tctaattcct ttgcttctcg attcagaacc
      901 ttggcgaatt caaaagccat ttcaggccta ctattgtaat gatcattggc aatggtatcg
      961 caaattgtca ttggtcctgg aagggtaaat tttattgctt tgtttgtgtt ttctcttaaa
     1021 aactgggcct cttttgagtg tattttttct attttcctaa ttgcttctgt gacggttgga
     1081 acttccacgg tataacgatc atttcttatc cccatcttga tttttttgtc ccaactaata
     1141 ccaagaatct tctgaagaaa tccatgcaca aaatgaattc taaattgttc accttcagaa
     1201 ataatttcca gcccagcttt ttcttgttct agtatccatt ctaaagcaga tttttgttga
     1261 cctttccaca attgttcatc agccagtttc catttaggcc acagtgtttc tggttcagca
     1321 agccaatttg gttttggcaa acttcctgcc gttgttgcca ataattttct ggtcataatt
     1381 ttccaagtta attgttaaac tcgctgttca cttattgcgt taaaatgaga ttaggaagta
     1441 gggattataa aatatttata tatttaagag aaccatatgg gaattagatg cggcattatc
     1501 ggattaccaa atgtaggtaa atcgactata ttcaatgctt taacacggag tgaggttgag
     1561 gttgccaact atcctttttg taccattaac cccaatatag gaagcgttgc tgttccagat
     1621 aaaagactaa gtgtttttgc tgatatgacg catatagaaa aagtcattcc ttcgttcgta
     1681 gaatttgtcg acattgctgg tttagtcgca ggtgcttcaa aaggtgaagg cttgggtaat
     1741 caatttcttg gtaatattag agaaacagat gccatagtcc atgtagttcg atgttttgag
     1801 aataatgata tagcccatgt ttcagaaaat attgatcctg taaatgatat tgaaattatt
     1861 aatacagaat tacttctggc agatatacaa acggttgaca aacattatgt ccgtgcagag
     1921 aaacaatcga aagcagccca acaaggagat cttgagaaga gagatttttt aaaaaaatta
     1981 ttggagcatc tcaataaatt cttaccggca cgaaatttta tattagacga tgaaaagacg
     2041 gatttttgga tgaaagaatt acatttgtta tctgccaaac cgacactata cgtggctaat
     2101 attaatgaag agaatgttca ttctggtaat gatttcatcg ttcaagttca agaatattta
     2161 ggccaccatc aagattttga attgattaca atttgtgggg tattggaatc tgaactatct
     2221 caaattgaag gcgaagaaca aattgaattt ctgaaagatc ttgagttaga ggaacctgca
     2281 ttagagagat taatccaatc atgctatacc ttacttggtt tgcaaacatt ttttaccaca
     2341 cgccctggag aggtaagatc ttggcctgtc aaagtcaaga ccaaagcacc acaagctgca
     2401 ggccttattc atagtgattt tgaacgtggt tttatctccg ctgaagtcat tccatataga
     2461 gattatgttg agctgggtgg ggagcaggct tgcaaaactg cgggtaagct tagactagaa
     2521 ggtaaagact atattgtcgg tgaaggtgat gttattcatt ttcgttttaa tgtttgacga
     2581 tagaattttt tactctctgc tgagacctgc tatagcatca attattgttt cataattctc
     2641 gtaaaattct ttggattcgc catcagcttg agtgatgggt gtattaatat cctcggttag
     2701 atcaattaga cctaaattgt ttaagaaacg agtgatcttg atccagcgac taatatttct
     2761 aacattatgc ccacctaaag tgccccaatc ttgaacagag cctggagtgg ggtggtattt
     2821 tgttattctt gtaaaaataa catcattttt gcgatcaaca aatatatatt gcccaaattt
     2881 accgctggca ttaaaaataa tgccttcatc atcgttcttg gacatccacc agtggagatt
     2941 atagccgcgt tgtatttgac tagtgattcc acctggggtt gtatcccagt atacggtgaa
     3001 tgtttcatct acaaattctt ttgagacaat tgtttgttcg ttccattgtc catttcttga
     3061 aaagagcaac ccaaatcgtg aaaagtctct tgcagacatg tctatgcagc agtaagaaag
     3121 aacattgcct actgcatcac gccacagggt atggtcaccc acaccaattg gatctagaat
     3181 tctctgtttc aatagtacat cagcttttct gcctgtggct tttaataaaa tgtcacccaa
     3241 caacatagaa tttgcattat tgtattcaaa gattttattt ggttgtttct caaccccaac
     3301 atcttttgca taagcaaggt gatctgtttc aaagaacata agctcgtgct catgttcagg
     3361 gaactgaagt ccagatgaca tattgagaag atcccttatt tttatctcag atcttgcatc
     3421 attgaagtat tctaaataat cactcacttt atcatcgagg ctattgatct caccttgatc
     3481 aattgaaata ccaataagcg ctgcatacaa gcttttcgct gctgaccacg atgtaccaaa
     3541 cgaagatgca tcaaagtctg ctgcatattt ttctccaatc aaaaatccat tcttaattaa
     3601 aaccactgcc tgcgttgctg gatcttgaaa cgataagtca aacagagtat ctattttttt
     3661 ttgatcaaca ttaagagtgc tcggtgtttt cgttgtccat tgatctgtag gatattccag
     3721 agtatcagca aataaaatgc tggatgaagc atataataag actaaaccac agtaacaaat
     3781 tagttttttt ctcatacgct taataacttc tgagctataa ttttggggct gaaacttgac
     3841 agaattagtt ttcagactga caatccattt ttctggtcaa tacggtgatg tagctcagta
     3901 ggttagagca tcggattcat aaaccgaagg tcggtggttc aaatccaccc atcaccacca
     3961 gaatgctggt ctaacccatt gggttattgg cattcaagaa ttcaggatcg attgtgtcaa
     4021 caccttggtc tttggcagca ttgaccatat ggttaatggc catatccatt gcaaaatctg
     4081 ggacctgtgt caatgcttcg caag
//
LOCUS       DASCTH010000256         1694 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000531113, whole genome shotgun sequence.
ACCESSION   DASCTH010000256 DASCTH010000000
VERSION     DASCTH010000256.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000531113"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="QGH35_05455"
     CDS             complement(<1..184)
                     /locus_tag="QGH35_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82011.1"
                     /translation="MSAVFLPIIVIVLFMLRQNLIVVLGIATAYAYWAFGDGVISNIV
                     VDAWDAANKDILLSIPL"
     gene            complement(181..669)
                     /locus_tag="QGH35_05460"
     CDS             complement(181..669)
                     /locus_tag="QGH35_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HJL82012.1"
                     /translation="MTRFEKGITFIAFAVLVVVMFTDVAIRELTGAGLHWSRQLGVYA
                     NLFVVMFGLGVASSEGAHLRPKFADAWLPKNWDPVLIRVQEGIMSLFCLCFAIIASQV
                     VIETFHDDVQSVVLRIVIWPFQAVIPLAFFMTSIRHFLYTIDPKNRPTVESSDELEGE
                     QR"
     gene            788..>1694
                     /locus_tag="QGH35_05465"
     CDS             788..>1694
                     /locus_tag="QGH35_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012398.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJL82013.1"
                     /translation="MANNEQISISEEVKRFLNSDHQLLIDGEWVSSSSGEKIPVFDPA
                     TEEQLTEVDSGNQTDIDAAVTAARKALTGEWSRINSHDRAQLLNKLADDIEANFSVLS
                     EMEVLDNGMPLALAQYSIAGHGVTLLRYYASWATKIHGDTIPVSPGGAANGESITYTR
                     KEPIGVVGAIIPWNSPLIFAILKIAPALAAGCTVVLKPAELTPITALFLGSLIQKSGI
                     PKGVVNIVTGYGEIAGTALTHHDNVDKIAFTGSTEVGKKIVEASVGNLKKVTLELGGK
                     NPVVVFPDADLEKAIPGAARACFFLQ"
BASE COUNT          518 a          330 c          353 g          493 t
ORIGIN      
        1 aaagaggaat tgaaagcagg atatccttgt ttgccgcatc ccaagcatca accactatat
       61 tgcttataac tccatctcca aaagcccagt atgcgtaggc tgtggcaatc ccgagtacga
      121 caattaggtt ttgccttaac atgaataaga caatgacaat aattggaaga aaaactgcac
      181 tcatctttgt tcgccttcta gttcatcact gctctcaact gtgggtctat tctttggatc
      241 aatggtgtag agaaaatgtc gaattgaggt catgaaaaag gctaaaggta tcactgcctg
      301 aaatggccaa atgactattc ttaataccac cgattgaaca tcatcatgaa aggtttctat
      361 gaccacttgg gaagcaatta tggcaaagca taagcaaaac aatgacatga tgccttcttg
      421 cactctgatc agaaccggat cccaattttt tggtaaccat gcgtcagcaa atttgggcct
      481 caagtgggca ccttcagatg aagcaacccc taagccaaac atcaccacaa ataaattggc
      541 ataaacccca agttgccttg accaatgcaa accagctcct gtgagctctc tgatagcaac
      601 atcggtgaac ataacaacca ccagtactgc aaatgcaatg aaagtaatgc ctttttcgaa
      661 tcgagtcatt acagttagag cttttttaat catgtggttt ttatttttta ttaatatggg
      721 gataaatctt atttcattgt aaactatgat ttaacagaaa tttaaaatgg attaaaaaaa
      781 ggtttttatg gcaaacaatg aacagatttc tatttctgag gaagtaaaaa gatttcttaa
      841 ctcagatcac caacttctca ttgatggaga gtgggtttct tcatcttctg gggaaaaaat
      901 cccggttttt gatccagcta ctgaagaaca actaactgaa gtagactctg ggaaccaaac
      961 tgatattgat gccgcagtta ctgctgctcg taaagcactt actggtgagt ggtctagaat
     1021 caactctcat gatcgtgcac agttgctgaa taaattggct gatgatatag aggcaaattt
     1081 ttcagttttg tcagaaatgg aggttttgga caatggcatg cccctggctt tggcacaata
     1141 ttccattgct ggccatgggg tgactttatt acgttattat gccagctggg cgaccaagat
     1201 ccatggggat actattcccg tttctccagg cggagccgct aacggagaat caattactta
     1261 tacaagaaag gagccgattg gagtggttgg tgccatcatt ccatggaact ctcccttgat
     1321 tttcgctatt ttaaaaatag ctcccgcttt agcagcaggt tgtacagtgg ttctcaagcc
     1381 agctgaatta actcctataa ctgcattatt tttaggtagt ttgattcaaa aatctggaat
     1441 tccaaaagga gttgtgaata tcgtgacagg atatggagaa atagcaggaa ctgccctaac
     1501 ccaccatgat aatgttgata agattgcgtt cacaggttca actgaggtgg gtaagaaaat
     1561 tgtagaagct tctgttggaa atctaaaaaa ggtcacgctt gaattaggtg gcaaaaatcc
     1621 tgttgttgtt tttccagatg ctgatttaga aaaagcgatt ccaggggctg caagagcttg
     1681 tttttttctt cagg
//
LOCUS       DASCTH010000257         2704 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000532101, whole genome shotgun sequence.
ACCESSION   DASCTH010000257 DASCTH010000000
VERSION     DASCTH010000257.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000532101"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="QGH35_05470"
     CDS             complement(<1..452)
                     /locus_tag="QGH35_05470"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of acetyl phosphate from
                     acetyl-CoA and phosphate; can also act with other
                     short-chain acyl-CoAs; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HJL82014.1"
                     /translation="MLSSVPFECPVSLLAQAQRHVPLKTAVVNAETELVMTSSKLATE
                     HNLIEPTLVGDPRTINSIAAAINWDIRKLAVVDAKNETTAAKLSVGLARNGEVSALMK
                     GHVHSETLMQEALRRNQGIRLRHRPSHAFYMTVPGHEQGICITDAVINV"
     gene            complement(468..2213)
                     /gene="xsc"
                     /locus_tag="QGH35_05475"
     CDS             complement(468..2213)
                     /gene="xsc"
                     /locus_tag="QGH35_05475"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HJL82015.1"
                     /translation="MTPSEAFVETMAAHGVTEIFGIMGSAFMDAMDIFEPAGIRFVSV
                     VHEQGAAHMADGYSRVSGRQGVCIGQNGPGISNCVTGIAAAFWAHSPVVIVTPETGTM
                     GIGLGGFQEANQLPMFQEFTKYQVHLNNPNRMAEFTARCFDRALSEMGPTQINIPRDL
                     FYGDINVEIPQPRCLDQGAGGEKTLNEAVKLLTNAQFPVIVSGGGVVNAGGVGACRLL
                     AERLGSPVVNSYLHNDSFPASHPLWCGPLGYQGSKAGMKLINQADVVLALGTRLGPFG
                     TLPQYGLDYWPDAANIIQVDADHRMLGLVKKISVGVCGDAKASAEVLLDRLDGISLAS
                     DKNKKERAKKIAAAKKEWEQELDGWIHEKDAFSEEAIKKAGRGFLHPRQVLRELEKAM
                     PEDVMVSTDIGNINSIANSYLRFEKPRSFFAAMSFGNCGYAFPTIIGAKVAAPDRPAI
                     AYVGDGAWGMSMGEVMTCVRENIPTTVVVFNNGQWGAEKKNQVDFYNQRFVAGELDNQ
                     SFANIARSMGAEGVTVNQLEDVGTALKTALDGQINDHKTTIIEIMCTQELGDPFRRDA
                     LSKPVRYLDKYKKYV"
     gene            complement(2289..>2704)
                     /locus_tag="QGH35_05480"
     CDS             complement(2289..>2704)
                     /locus_tag="QGH35_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HJL82016.1"
                     /translation="AIAVGASDALGFGANTRAALITRGLGEMKRIGLVFGARAETFFG
                     LSGLGDLVLTSTDNQSRNRQLGLLVGSGLSLDAAVKKLGATPEGVHAVKAIYKKGALQ
                     ESLPIAKAVYSILFEDVPAEGLVKQLMSRPIKEEH"
BASE COUNT          711 a          712 c          569 g          712 t
ORIGIN      
        1 acattaataa cagcatcagt tatacaaatg ccctgctcat gaccaggcac agtcatataa
       61 aaagcgtggc tggggcgatg ccttaaacga attccttgat tacgacgtaa ggcttcctgc
      121 attaaagtct ctgagtgaac atgacccttc attaatgcag aaacctctcc attacgcgca
      181 agacccacgc tgagcttcgc ggccgttgtt tcatttttag catcaactac agcgagcttt
      241 ctgatgtccc aattgatagc ggcggcaatg gagttgatag ttctagggtc gccgaccagc
      301 gtcggctcga tcagattatg ttccgtggcc aatttagagc ttgtcattac cagttcggtt
      361 tcagcgttaa cgactgcagt ttttaagggc acatggcgct gggcttgtgc aagtaatgat
      421 acgggacatt caaaaggtac ggagctcaac ataaaacgtt tctcggctca gacgtatttt
      481 ttgtatttat ccaggtatct aaccggtttt gataacgcat cacgccgaaa agggtcaccc
      541 aactcttgcg tgcacataat ctcaataatt gtggttttgt gatcatttat ttgtccgtca
      601 agggcggttt ttagagctgt tccaacatcc tcaagttggt tgacagtgac tccctctgct
      661 cccatggagc gagcaatatt ggcaaagctt tggttatcaa gctctccggc tacgaaacgc
      721 tgattataaa aatccacctg attttttttc tcagcgcccc actgaccatt attaaaaaca
      781 acaacagttg tgggtatatt ttcacgcaca caggtcatga cctcacccat gctcatgccc
      841 caagcgccat ctcccacgta ggcaatggca gggcggtcag gagcagcgac tttggcgcca
      901 ataattgttg gaaacgcata accacaattg ccaaaactca tagcggcaaa aaagcttcgg
      961 ggtttttcaa agcgcaaata actgttcgcg attgagttaa tattaccgat atcggttgag
     1021 accataacat cttctggcat tgctttttca agctcacgta agacttggcg cggatgcaga
     1081 aaaccccgac cggctttttt gatggcctct tcgctaaagg cgtctttctc atgaatccac
     1141 ccatcaagct cctgctccca ttctttctta gcggctgcaa ttttttttgc tcgttctttc
     1201 ttgtttttgt cacttgccag gctaatcccg tcaagcctat caagcagaac ctcagcagat
     1261 gctttggcat caccgcaaac accaacagag attttcttaa ccaaccccaa cattctatga
     1321 tcagcgtcca cttggatgat gttggcagcg tccggccagt aatcaagacc atattgtggc
     1381 agggttccaa acgggcccag tctggtgcca agcgccaaca caacgtctgc ttggttaatg
     1441 agcttcatgc ccgctttcga gccctgataa ccaagcggtc cacaccaaag aggatgactt
     1501 gcgggaaatg aatcattatg taaataacta ttgacgacgg gggagccaag acgctctgct
     1561 aacaacctac acgcacccac accgccggca ttgaccacgc cgcctcctga gacaatgaca
     1621 ggaaattggg cattggtgag tagctttacc gcctcgttca aggttttttc tccaccagcc
     1681 ccctgatcta aacatcgggg ctgtggaatc tccacattaa tatcaccgta aaacaggtcc
     1741 cttggaatgt tgatttgagt tggccccatt tcagagagag ccctgtcaaa acaacgtgca
     1801 gtaaactctg ccattcggtt tgggttgttg agatgcactt ggtatttggt aaattcctgg
     1861 aacatgggca actggtttgc ctcctgaaac ccacccaaac caattcccat ggttcctgtt
     1921 tcaggagtaa caatgaccac cggagagtgt gcccaaaagg cagccgcaat ccctgtgaca
     1981 cagttgctaa taccgggacc attttgacca atacaaacac cctgtcttcc gctgactcgg
     2041 gaataaccat cagccatatg tgctgcgccc tgctcgtgca caacggacac aaaacgaatc
     2101 ccagcaggct cgaaaatatc catggcatcc atgaacgcag agcccatgat tccaaaaatt
     2161 tctgtaacac catgggcagc cattgtttct acaaaggctt cactgggagt catgcggggc
     2221 atatgttgtt ctctttaatt aattcttttt tttgagccaa taagacaaaa agctttcacc
     2281 gaaccgttct aatgttcttc ttttataggc cgagacatta attgtttcac caagccttca
     2341 gcaggaacat cttcaaataa aatactatac accgctttgg ctatcggcaa gctttcttgc
     2401 agagctcctt ttttgtaaat tgctttaact gcatgcaccc cttcaggagt tgccccaagc
     2461 tttttaacag cagcatccag tgaaagcccc gagccaacta aaaggcccag ctgtctgttc
     2521 cgggactggt tgtcggtaga ggttagcact aaatctccca gcccagaaag accaaaaaat
     2581 gtttctgccc ttgcgccaaa aaccaaacca attcttttca tttcgccaag ccctcttgtg
     2641 attaaagcag ccctcgtgtt tgccccaaac ccaagtgcgt cagaggcacc aaccgcaatg
     2701 gcaa
//
LOCUS       DASCTH010000258         2590 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000535853, whole genome shotgun sequence.
ACCESSION   DASCTH010000258 DASCTH010000000
VERSION     DASCTH010000258.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000535853"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            16..1788
                     /locus_tag="QGH35_05485"
     CDS             16..1788
                     /locus_tag="QGH35_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HJL82017.1"
                     /translation="MTEEIIRIANCSGYYGDKLSAAKEMVEGGPIDVLTGDYLAELTM
                     AILYSQKLQRGEDKGYVGTFLKQLKEIAKTCKDQKIKIVSNAGGLNPKSMAKEVETIL
                     AELNVDATVAYIDGDDLIEELERLQAAGETLENIDRKIPLAQSDCKVLTANTYLGSWG
                     IKEALDLGADIVICPRVTDASVTMGPAAWKFNWQRDDYDALAGSLAAGHIIECGAQAT
                     GGNYSFFEEVPTFKNLGYPIAEIEEDGSFTITKHPGTGGLVSIGTVTAQLLYEIAAPE
                     YLNPDVIAHFDTLKMQQVGDDRVRVTGCKGSSPPSTHKVCINLAGGYRNGMELIITGL
                     DIEKKAKIFTEALFDLLGGKEQFDDVAVELHRQEKENPQSNEEAMASLRVNVTSKDPN
                     KVGRIFTAKVVELGLANYPGFFGKSPPGSGAPFIFYWPALIHSKHVEETVHIGEQTVK
                     ITPTNQLGLDKHTYQKEEQKIPQLVSKNHQPIHFGRIYGTRSGDKGGCANLGVWAKTD
                     RAFEFLYYFLTVDQLKQLLPDIANFPIDRYELANIKSLNFYIHEILQDGVSSNTRMDA
                     QAKSLGEYLRAKLIDVPVEIIEEL"
     gene            1789..>2590
                     /locus_tag="QGH35_05490"
     CDS             1789..>2590
                     /locus_tag="QGH35_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HJL82018.1"
                     /translation="MNDSTNLLSLEGLVFEAIEINIDGHILSITLNRPEKRNAINTVM
                     GNEITYALSYASQESNVRVVIIQAKGDVFCAGGDLGGMLGKEQQTTSTVPKLGDTSNI
                     SLLIRNLCKPLICKIQGPAYAGALLMICNATHAIAADHVEFATPEIKRGIWPFMVMAG
                     LFRVMPKRQGLDFIMRGETIDALTAEKYGLITQAVAKDELDETVSKLANDFANLPPNT
                     MRIGLEAYNRQDAMAFDDALPYLQKQLNKCLGTEDAKEGIAAFFEKREP"
BASE COUNT          820 a          501 c          596 g          673 t
ORIGIN      
        1 agctgagaaa tatttatgac tgaagaaatt atcaggattg ctaattgcag tggttactat
       61 ggtgacaaac tctcagctgc taaagagatg gttgagggtg gtcccattga tgttttaacc
      121 ggagattatc tcgctgaact aaccatggct attctctatt ctcaaaaatt gcagagaggt
      181 gaagataaag ggtatgtggg gacatttttg aagcagctca aagaaatagc caagacgtgc
      241 aaagaccaga agattaaaat tgtctccaat gcagggggtc tcaacccaaa atcaatggcg
      301 aaggaagttg aaaccattct tgccgaattg aatgttgatg caactgttgc ctatattgat
      361 ggggatgatt taatagaaga actggaacga ctccaagctg caggcgaaac attagaaaat
      421 attgatcgaa aaataccctt ggcgcaatct gactgtaaag ttctgactgc aaatacttat
      481 cttggcagtt ggggaataaa agaggcattg gatttgggtg cagacatagt gatttgcccc
      541 agagttactg atgcatcagt aaccatggga ccggcggctt ggaaatttaa ttggcaaaga
      601 gatgactacg atgcacttgc cggatcattg gcagctggtc atatcataga gtgcggagcc
      661 caagcaactg gtggaaatta ctcgttcttt gaagaagtgc caactttcaa aaatttagga
      721 tatcccattg ctgaaataga ggaggatggc tcttttacca ttaccaaaca ccccggtact
      781 ggtggtcttg tttctatagg tacagtaacg gcccaactac tctatgagat tgctgcaccg
      841 gagtatttga atccagatgt aattgcccat tttgacacct taaaaatgca acaggtaggt
      901 gacgacaggg ttcgtgttac gggttgcaag ggcagtagcc caccgtctac tcataaggtc
      961 tgtattaatc ttgccggcgg ttatcgaaat ggaatggagt tgatcataac tggcttagat
     1021 attgaaaaaa aggcaaaaat atttaccgaa gccctatttg acttattagg tggtaaagag
     1081 caatttgatg atgtcgctgt tgaattgcat aggcaagaaa aagaaaaccc tcagagcaac
     1141 gaggaggcaa tggcatcttt gcgggtcaat gttacatcca aagatccaaa caaggtgggt
     1201 agaattttca ctgccaaggt tgttgaattg gggcttgcca attatccagg attttttggt
     1261 aaatcaccac ccggttccgg agccccgttc attttttatt ggcccgcctt aatccattca
     1321 aaacatgttg aggaaaccgt tcatattggc gaacaaacag tgaaaattac cccgaccaat
     1381 caactgggac tggataaaca cacatatcag aaagaagaac aaaagattcc ccagttagtg
     1441 agcaagaacc accaaccaat ccattttgga agaatctacg gtacacgatc tggtgataaa
     1501 gggggttgtg ccaatcttgg tgtctgggct aaaactgaca gagcctttga atttctttac
     1561 tatttcctga ctgtcgacca attaaaacaa ttactccctg acatagctaa ctttccgatt
     1621 gatagatacg agcttgccaa tattaagtcc ttaaactttt atatccacga gatcttgcaa
     1681 gacggagtct cttccaatac ccggatggat gcacaggcaa aatctcttgg tgaatactta
     1741 cgggccaaac tgatagatgt accagtagaa attattgagg agctataaat gaatgattca
     1801 acaaacctgt tgtctcttga aggattggtt tttgaggcaa ttgaaataaa catcgatgga
     1861 cacatactgt caattactct aaatcgacca gagaaacgca atgcgatcaa cacggttatg
     1921 gggaatgaga taacctatgc tctttcctat gcatcgcagg aaagcaatgt cagggttgtc
     1981 atcattcaag ccaagggaga tgtcttttgt gctgggggag atttgggtgg aatgttaggc
     2041 aaagaacaac aaacaacttc caccgtacct aaacttgggg atacatcaaa catcagttta
     2101 ttaatacgta acctgtgtaa accattaatc tgcaagatcc aaggaccagc ttatgccggg
     2161 gcactgttga tgatctgtaa cgcaacccac gcaattgcag cagatcatgt agagtttgca
     2221 accccagaaa tcaaacgagg catctggccg tttatggtaa tggcaggatt attcagagtg
     2281 atgcccaaga gacaaggttt ggattttatt atgagaggag agacaatcga tgcactaacc
     2341 gctgagaaat acggtttaat tacccaagca gtggctaaag atgagcttga tgaaactgtt
     2401 agtaagctgg caaacgattt tgcaaacctg ccaccgaaca ctatgagaat aggtttggaa
     2461 gcttataatc gtcaagatgc tatggcgttt gacgacgcac ttccatattt gcaaaaacaa
     2521 ctaaacaaat gcttagggac cgaagatgca aaagagggca ttgccgcatt ctttgaaaaa
     2581 cgagaaccgc
//
LOCUS       DASCTH010000259         1981 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000538309, whole genome shotgun sequence.
ACCESSION   DASCTH010000259 DASCTH010000000
VERSION     DASCTH010000259.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000538309"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="QGH35_05495"
     CDS             complement(<1..623)
                     /locus_tag="QGH35_05495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL82019.1"
                     /translation="MYFGLSEEQEFFQESLLKFLNDAAPVESIREYANGNHENLTQDI
                     HQGLLDLGVNALIIPEDFGGLGLDLLFAAAVSQSLGSGIAPATYIGSHIMAPLAIMEA
                     GSGDQKDEYLSAMANSKMRFGIGITEFIGAREDAGIVLESGKASGRTLFVIDGAGATH
                     YLLSDNKGVLFVVDAKDSGITKIPLMTIDKTRTSLELVLEDVPTEIL"
     gene            complement(626..1867)
                     /locus_tag="QGH35_05500"
     CDS             complement(626..1867)
                     /locus_tag="QGH35_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL82020.1"
                     /translation="MNLDYGPEYEDFRKEVEDFCKKYQGLEFSDALDSSQVPVGGGKK
                     SDNKNSLIMTRSEWQKILIEHGYFAREIPKEYGGYGGTIDILKNKIINSEFNKAGVPV
                     ATRGQGIDFLVPTLLEIGTEEQKQKYIKPTLFFEMIWCQGYSEPNSGSDLASLQTKGV
                     LDGDSWVINGQKIWTSTAKYADMMFCLVRTEPDAPKYQGISYLLIPMDTPGIEIKPLV
                     DMTLNASFNEVFFTDVRIPAGNIVGERGQGWLVANKTLIHERGSLGDPELIVSRLSQL
                     LELMKNETIDGTRIIDKPVFRDRAMKIQGRVLAFQNHGLRLLSARLNKRKDVMLGIMV
                     LKLIGTELRYELEGLGIDVMGELGTLYEDSSNLRDDGSWQFAYMMYLGLIIGGGTSQI
                     QKNIIAERGLGLPKAPKVKEA"
BASE COUNT          518 a          476 c          356 g          631 t
ORIGIN      
        1 ttgaggatct ctgtgggaac gtcctctaac actaattcaa gtgaggttcg tgttttatca
       61 attgtcatca gtggtatttt agttattcct gaatcttttg catcgactac aaaaagcacc
      121 cctttgttgt ctgacaatag ataatgtgtt gctccagctc catcgatcac aaaaagagtc
      181 cttccactgg ctttacccga ttccaaaaca atgcctgcat cctctcttgc tccaatgaat
      241 tctgtgatcc ctattccaaa acgcatcttg gagttagcca tagcgctcaa gtattcatct
      301 ttttgatcac ccgatccagc ttccataatt gcaaggggtg ccataatgtg ggagccgatg
      361 taggttgccg gtgcaatacc tgaaccgaga gattgtgata cagctgcagc aaacaataaa
      421 tccaatccaa gaccaccaaa atcctcaggg ataatgagtg cattgacacc aagatcaagc
      481 agtccttgat ggatgtcttg tgttaagttt tcatgattcc cattggcata ctccctgatt
      541 gattcaactg gagcagcatc attgagaaat tttaataagc tttcctggaa aaattcttgt
      601 tcttctgaga gtccaaaata catggctagg cttccttcac tttaggtgct tttggaagtc
      661 cgagacctcg ttcggcaatg atattttttt gtatttgact ggtgccacca ccaatgatta
      721 atcccaaata catcatataa gcaaactgcc aactgccatc atccctaagg ttggaactgt
      781 cctcataaag cgttcccagc tctcccatca catcgatccc caggccttcc agctcatacc
      841 tgagttctgt tcctattagt tttaggacca tgatgccaag cataacgtct tttcttttat
      901 tcagtcgagc tgacaacaat cttaatccat ggttctgaaa agccagaacc cgaccttgaa
      961 ttttcatcgc tcgatcacgg aaaacgggct tatctatgat tctcgtacca tcaatagtct
     1021 cgttcttcat caactcaagt agttgactca acctactcac aatcagctct ggatctccca
     1081 gagagcccct ctcatgaatc agtgttttat ttgctaccag ccagccctgg cctctttcac
     1141 caacgatgtt accagcaggg atccttacat cagtaaaaaa gacttcgtta aaactggcat
     1201 tcagggtcat gtcaaccagg ggttttattt caataccagg ggtgtccatt ggaattaata
     1261 aataactgat cccttgatat tttggtgcat ctggttcggt tcggaccaga caaaacatca
     1321 tgtctgcata tttggcagtg ctggtccaga tcttttgacc gttgatgacc caactgtccc
     1381 catccaaaac acctttagtc tgtaggctgg ccaaatcgct gcctgaattt ggctccgagt
     1441 agccttggca ccaaatcatt tcaaaaaaga gcgttggttt aatgtatttt tgtttttgct
     1501 cttctgtccc tatttcaaga agtgtgggca ctaaaaaatc gatgccctgt ccccgggttg
     1561 caactggaac tcctgctttg ttgaattcag agttaattat tttattcttc agaatatcaa
     1621 tggttccacc ataaccacca tattctttag gtatctccct tgcaaagtat ccgtgctcta
     1681 tcagaatttt ctgccattca gaacgggtca taatcaggct atttttgtta tctgattttt
     1741 tacctccccc cactggaact tgcgaagaat ccagagcgtc gctaaactct agaccctggt
     1801 attttttaca gaaatcttcg acttccttgc gaaaatcttc gtattcagga ccataatcaa
     1861 gattcatttt atgttctcat tgagtcaaat ttataccttt ttaggaacac ccttttctga
     1921 tacttcatta tcatgatgtt tttttgcttc aatgagttct ggcatcaatt tatcgagttg
     1981 t
//
LOCUS       DASCTH010000260         3173 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000538585, whole genome shotgun sequence.
ACCESSION   DASCTH010000260 DASCTH010000000
VERSION     DASCTH010000260.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000538585"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="QGH35_05505"
     CDS             <1..433
                     /locus_tag="QGH35_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJL82021.1"
                     /translation="PALGYRFDFKGRSIVISGDTSYSENLITHAKDADVLFHEAQANH
                     ILEMMKGVFDKAGDPLTAKILDDITTYHTTPKDAAKVANLANVDHLVFYHLTPSPRNS
                     IMERIFFRGVNKIREDWSVVDDGTMVVLPLNSKDIIISSIN"
     gene            490..1278
                     /locus_tag="QGH35_05510"
     CDS             490..1278
                     /locus_tag="QGH35_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HJL82022.1"
                     /translation="MHKISLYQVDAFTNKIFKGNPAAVCPLEEWLSDEVMLSIASENN
                     LSETAFVNVNSSPFFIRWFTPITEVDLCGHATLATARILFDEYLPKNISEIIFNSKSG
                     ELKAFKKDELIYLDFPKDEPRQVEGYSLITQGLGKQPMDVFKGKDDFLAIFEDEQVIK
                     SMNPNFKKISELDSRGLIISARGVSEDFVSRCFYPQSGIDEDPVTGSAHTLMVPYWAA
                     VLQKNKLLACQYSSRGGFLKCQLLGDRVLIGGASVRYMQGKITL"
     gene            1359..1781
                     /locus_tag="QGH35_05515"
     CDS             1359..1781
                     /locus_tag="QGH35_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJL82023.1"
                     /translation="MKYLHTMIRISDVKSSLDFYCNQLGLQELRRVDNEAGRYSLIFL
                     SAPGDESAQIELTYNWDGDELGEGSRNFGHLAYSVENIYDTCRRLQEAGVIINRPPRD
                     GYMAFVRSPDNISVEFLQSGKPLPPEEPWISMDNTGNW"
     gene            1785..2960
                     /locus_tag="QGH35_05520"
     CDS             1785..2960
                     /locus_tag="QGH35_05520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020396692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HJL82024.1"
                     /translation="MASKRTLESCHKVMDFRKLAEKRLPAPMFHYIDGAAEDEWTYRQ
                     NTSAFNQLELIPRFLVNVENIDMSTTVLGQKIDWPFFCSPTGYCRLFHHDGEGGAARA
                     AARTGTIFCLSSIATTSIEEVATASDGPKIFQIYVLKDRGLSQEYIQRCKDANYDALC
                     LTVDVPSNGKRERDLVTGMTVPPKLGLSGYMNVMKHIDWSFNYLTHPPTPSMVNIEHK
                     LPKGSSSISVMDFMNTQFDRTVTWKDAAWIIGQWDKPFAIKGILSVEDAKMAVEIGAS
                     AVMVSNHGGRQMDGCPAPIEVIGDIVDAVGDKLEVILDGGVRRGTHILKALAMGAKAC
                     MGGRAYLYGLAAGGEEGAYSSLKILKDEVQNNMILLGCTDISQISSDYLRKTESRLP"
BASE COUNT         1019 a          518 c          686 g          950 t
ORIGIN      
        1 accagcatta gggtatcgtt ttgatttcaa aggcagatct attgttatta gtggagatac
       61 ttcatatagc gaaaacttaa ttactcatgc aaaagatgcg gatgtattat ttcatgaggc
      121 tcaagctaat catattttag aaatgatgaa aggtgttttt gataaggctg gtgatccatt
      181 aacagcaaag attttggatg atattacgac ctatcacact actcctaagg atgcagctaa
      241 agtagcaaat cttgcaaatg ttgatcattt agttttttat catttaactc catctccaag
      301 aaatagcatt atggaaagaa tattttttcg gggagtaaat aaaataagag aagattggag
      361 tgtagttgat gatggaacta tggtggtttt acctttgaat tcaaaagata taatcatttc
      421 aagcatcaac taggaagaac tagaactttt tggacttagt gccttggtca ttaattgaat
      481 agagcagtaa tgcataaaat ttccctatat caagttgatg ctttcaccaa taagattttt
      541 aaaggaaacc ctgcagcagt ttgtccactc gaagaatggt tgtctgatga ggtaatgtta
      601 tcaattgcat cagaaaataa cctgtctgaa actgcttttg ttaatgtaaa ctcatccccc
      661 tttttcatcc gttggtttac accaataacc gaagttgatt tatgtggtca tgcaacactg
      721 gcaacagcaa gaattctttt tgatgaatat cttccaaaaa atatcagtga aataatcttt
      781 aattctaaga gtggagaatt aaaagcattc aaaaaggatg agttaattta tctagatttt
      841 ccaaaagatg agcctagaca agtggaagga tattctttga ttactcaagg gcttgggaag
      901 caaccaatgg atgtgtttaa gggtaaggat gacttcttag ccatcttcga ggatgagcag
      961 gtaataaagt caatgaatcc taactttaaa aaaatttcag agttggattc aaggggttta
     1021 attatcagtg ctcgaggcgt tagtgaggat tttgtatcga gatgttttta cccccaatca
     1081 gggattgatg aagatccagt aactggctcc gcacatactt tgatggttcc ttattgggca
     1141 gcagtgctac aaaagaataa attattagct tgccaatatt cttcgagagg tggatttcta
     1201 aagtgccagt tattggggga tagagttctt attggcggtg cttcagtgag atatatgcaa
     1261 ggaaagatta ctctttgaat ccttttatgt gtaaaacttg tacactacta tttaaataga
     1321 gagtaaaaat aatcatagaa aactaaggta tataaactat gaaatattta catacgatga
     1381 tacgtattag tgatgtcaaa tcttcccttg atttctactg taaccaatta ggtttacaag
     1441 aactaagacg agttgataat gaagcaggta gatatagtct tattttcctt tccgcacccg
     1501 gtgatgaatc tgcccaaata gaactaacct acaactggga tggggatgag ctaggagaag
     1561 gctcaagaaa ttttggacat ttagcatata gtgttgagaa catatatgat acatgcagac
     1621 gtttacaaga agccggagtg ataatcaatc gtcctcccag agatggatat atggcattcg
     1681 ttagatctcc agataatatc tcagtagaat ttctgcaatc aggcaaacct ttgccacctg
     1741 aagagccatg gatttcaatg gataatactg ggaattggta ataaatggcg tctaagcgaa
     1801 cattagaatc ttgtcataag gtcatggatt ttagaaagct tgctgagaag cgactccctg
     1861 cacctatgtt tcattacatc gatggagcgg ctgaagatga atggacttat cgtcaaaata
     1921 cctctgcttt caatcagctt gagttgatcc ctcgtttttt ggttaacgtc gagaacatag
     1981 acatgtctac aactgttttg gggcaaaaga tagattggcc atttttctgc tcgcccacag
     2041 gttattgcag gctcttccat catgatggag aagggggagc agctagggct gctgctagaa
     2101 ctggaactat tttttgtctt tcatcaattg ctaccacttc cattgaggag gtagcaacag
     2161 cttcagatgg gccaaaaata tttcagatct acgtcctaaa ggatagaggt ctctctcagg
     2221 aatatattca acgctgtaaa gatgccaatt atgatgcttt atgtctaacg gttgatgttc
     2281 ccagtaatgg taaacgagaa agagatttag taaccgggat gacggtgcca cccaaactag
     2341 gcctcagtgg ctatatgaat gtcatgaaac acattgattg gtcttttaat taccttactc
     2401 acccaccaac accttccatg gttaatatcg aacacaagtt acccaaaggg tcatcaagta
     2461 tctctgtgat ggattttatg aacacgcaat ttgatcgaac tgtcacttgg aaggatgcag
     2521 catggataat aggtcaatgg gacaaaccct ttgccattaa aggcattctc tctgttgaag
     2581 atgcaaaaat ggcagtagaa attggtgcat cagctgtgat ggtttcgaat catggaggca
     2641 gacagatgga tggatgtcct gcaccaatag aagttatcgg agatattgta gatgctgttg
     2701 gggacaaatt agaggtgatt ctcgatggtg gagttcgtag aggaacccat attctaaaag
     2761 ctctagctat gggcgcgaaa gcctgtatgg gtggaagagc ttatctttat ggattagcag
     2821 ctggtggaga agagggtgct tactcttcgc ttaagatatt aaaggatgaa gtacagaaca
     2881 atatgatttt attgggctgt acagatatca gtcagattag ttctgattat ttacgtaaga
     2941 cagaatcccg attgccttga ttatctaaaa tcgacgcaaa ctgtagctta tgaaaaactt
     3001 ctatatgaca gttatgttca cacttttggt gggtgctgcg atgaaaatga ataaactggg
     3061 aataatatga atactcagaa gagatctgaa ttaattgagc tttataatcg attagaaaaa
     3121 actctcgata agagcagaaa agaagcgaga gcaaaaagac atcaaagagg aaa
//
LOCUS       DASCTH010000261         2626 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000545016, whole genome shotgun sequence.
ACCESSION   DASCTH010000261 DASCTH010000000
VERSION     DASCTH010000261.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000545016"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            190..2214
                     /locus_tag="QGH35_05525"
     CDS             190..2214
                     /locus_tag="QGH35_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012888.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HJL82025.1"
                     /translation="MATNELAALKASFSRAKDFLNAGDAKMAEKICRSGLKNISSGDP
                     NLQVLLCVTLIRQGRSGSAVKRLKHTLRMFPDFEQAHEELGNALLAQNKPEKAIECFK
                     KAIEINPENVNSHIKLGKILHALGRVEEANEYYQQALKLDPIQEKLATTTQLISQNKL
                     EEAEKICREALQEKPDDVNGLRLLASIASKLEQNEDAIVLLKRAVELKPKFVAAWSAL
                     AEAYSDKNQFGEALDAVQRSIKLKPDLPFGFMIRGGILAKMNDHEGAITAYQEALKID
                     PDHVGSNMGLGNTLKTVGRFEESVTAYKKCIEVHPTFSEAYWSLANLKTYQFEPPEIN
                     LMENHLNNKDIPEQSIAFFHIALANVKETKKQYEKAWNHYLSGNTLRRKNEVYDSVQT
                     QITHDEVINTFTAEFLNQKTGLGHNSNEPIFIVGLPRSGSTLIEQILASHSLVEGTKE
                     LPDLSKLGVKLSKSRTRGMKYPNAVTDMSDAEFSDYGKSYIQTTKRYRTDKPYFIDKM
                     PNNFAHIGFLKLILPNAKVINATRHPLDSCMSSFKQLFYKGQSWSYDLFEIGEYYLEY
                     DRVMKHWQEILPSEILNLEYEKLVEDQEHETKRLLEFCGLDWEEHCLKFYETKRSINT
                     ASSEQVRQPIYKGAINSWRHYEKYIGPLIEILEPILMELPKKDRPKSLQK"
     gene            complement(2216..>2626)
                     /locus_tag="QGH35_05530"
     CDS             complement(2216..>2626)
                     /locus_tag="QGH35_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HJL82026.1"
                     /translation="FCDGWPEIKVSKESGWEKLSETALPIMKDVIDRFYEPGGTILRA
                     MAAKLLAGERITPHTDKHPSFHVAHRIHVPITTNDHVRFTIAGRPYQFELGHVYEINN
                     QKTHSVMNRGKEDRITFIFDYMPPDIRSKAKTLS"
BASE COUNT          905 a          503 c          503 g          715 t
ORIGIN      
        1 ccacattgcc gatgttgatt ttttcaaaag tacgcctagg cattgctcca gatatcaacg
       61 cacttgcaac aattctgata gttgtcgtca ccatcggagt atccatttca ggtttggtaa
      121 tgcatcggca aagcaagtca aaaaaagtgt gaatttttag caagataata cctcaaaaca
      181 aggacaagta tggctaccaa tgaacttgca gcactcaaag catcctttag cagagctaaa
      241 gattttctca acgctggtga tgcaaaaatg gctgagaaaa tttgtcgctc tggactaaaa
      301 aatatttcca gtggtgatcc aaatttgcaa gttttgttat gtgtgaccct gatccgacaa
      361 ggtcggtctg gatcagcagt aaaaagattg aagcatacat tgagaatgtt tcctgacttc
      421 gagcaagcac atgaagagct tggtaatgca cttctagccc aaaataaacc tgaaaaagcg
      481 atagagtgct ttaaaaaggc aatcgaaatt aatccagaaa atgtaaattc tcatattaaa
      541 ttgggaaaaa tccttcatgc cctaggaagg gtagaagaag caaatgaata ttatcagcaa
      601 gctctaaaat tagatccgat tcaagaaaag ctggctacaa caacacaact catcagtcaa
      661 aataaattag aagaagcaga aaaaatttgc agagaagctc ttcaagaaaa gccagacgat
      721 gtcaatggtc taagattact tgcctcaata gcatcaaagc tagaacaaaa tgaagacgcc
      781 attgtgcttc tcaaaagagc cgttgaactt aaaccaaaat ttgttgctgc atggtctgca
      841 cttgcggaag cctactctga taaaaatcaa ttcggtgaag cccttgatgc agtccaaaga
      901 tccataaaat taaaacctga tctccctttt gggtttatga ttcgaggcgg catactggcc
      961 aaaatgaatg atcatgaagg agccatcact gcttaccaag aagccctcaa aatagaccct
     1021 gaccacgtag gaagcaatat gggtttgggt aatacattaa agactgttgg ccgttttgaa
     1081 gagtccgtca ctgcttataa aaaatgcatt gaagtacatc ccactttttc agaagcttat
     1141 tggagtttag ctaatcttaa aacctaccaa tttgaacccc cagaaataaa cttgatggaa
     1201 aatcatctaa acaacaaaga cattcctgaa caaagcattg cttttttcca catagctttg
     1261 gcaaacgtca aagaaacaaa aaaacaatat gagaaagctt ggaatcatta tctcagtggc
     1321 aatacattga gacgaaaaaa tgaagtctac gactcagttc aaacacagat cactcacgat
     1381 gaagtcatta atacgtttac tgcagaattt ttgaatcaaa agacaggact tggtcacaat
     1441 agtaatgagc caattttcat tgttgggctg ccaagatctg gctcaacgct gattgaacaa
     1501 attcttgcga gccatagttt ggttgaagga acaaaagagc tcccagatct ttctaagtta
     1561 ggcgtcaaac tgagtaaatc aagaactcga ggaatgaaat acccaaatgc cgttaccgac
     1621 atgtcagatg ctgaattttc agattacgga aaaagttata tacaaacaac gaaaagatat
     1681 aggactgata aaccttattt cattgataaa atgcccaata attttgccca cataggtttt
     1741 ctcaaactga tactgccaaa tgcaaaagtc attaatgcta caagacatcc tctagacagt
     1801 tgcatgagtt cctttaagca gcttttttat aaaggccaat cttggagtta tgacttattc
     1861 gagataggag agtactacct ggagtacgac cgggtgatga agcattggca agaaatttta
     1921 cccagtgaga ttcttaatct tgaatatgaa aaattagttg aagatcaaga acatgaaacg
     1981 aagagactgc ttgaattctg tggtctggat tgggaagagc attgcctgaa attttatgaa
     2041 acaaaacgct cgatcaatac tgcaagttca gaacaagtcc gccaaccaat ctataaagga
     2101 gcaatcaatt cttggcgcca ttatgagaaa tacattggac cattgattga aattcttgaa
     2161 cccatactca tggaactacc taaaaaagat aggccaaaaa gtttgcaaaa atagtctagc
     2221 tcagagtttt tgcctttgaa cgaatgtctg gtggcatgta gtcaaagata aacgtaattc
     2281 tatcttcctt gcctcgattc atcacgctat gggttttttg gttattgatc tcatagacat
     2341 ggcccaactc aaattgatag ggacgtccag caattgtaaa acgaacgtga tcattagttg
     2401 ttataggaac gtgaattcga tgagcaacgt ggaaagatgg atgtttatca gtatggggag
     2461 taattctttc tcctgccaat aattttgctg ccatggctct taatatcgtt ccaccgggct
     2521 cataaaatcg gtcaattaca tctttcataa taggtaatgc tgtctcagat aatttttccc
     2581 atcctgattc ttttgatact tttatttcag gccatccatc gcaaaa
//
LOCUS       DASCTH010000262         3597 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000546083, whole genome shotgun sequence.
ACCESSION   DASCTH010000262 DASCTH010000000
VERSION     DASCTH010000262.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000546083"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(11..901)
                     /locus_tag="QGH35_05535"
     CDS             complement(11..901)
                     /locus_tag="QGH35_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024109.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJL82027.1"
                     /translation="MNRRQLLFSALLISTSLIGSFDLGVNAAMPGMAGDIQRSYSNTR
                     FGRVHHWSIGQGSTLLMLHQSGQASTEFLAIAPLLADSFTVVGIDLPNHGQSDTSDHE
                     LTVDEYADAVIDVLDNMGITQAHMLGQHGGAVVVINLGIRYPERVGKVILSGAGHDEN
                     VSEEELQKKLNTPMTRDLPIDVDGDFLQKTWSVYRKMSASNTPPEVTFQPFLSSLTLR
                     QRRYDLHYAVYRYSPNLDEFNKETLLLKAEEDIYAGDVAALQRRIPGSILRNIPNCGS
                     WQFFEQPELHAEIIQEFLGQ"
     gene            complement(984..3476)
                     /locus_tag="QGH35_05540"
     CDS             complement(984..3476)
                     /locus_tag="QGH35_05540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HJL82028.1"
                     /translation="MTIRESFSFLVNSAIMVVITIALMPTVTFSQEGIEEIVVTARKK
                     EETILETPLAVTALSAQDIKDSAFQNILDIQKSTPGFFIEAINNTNARVMAMPRFRGV
                     TFDTTSPLARTGSVFIDGVLVTGGLHSLPVSNAERVEIIKGPQSAVFGRNTYSGAINI
                     ITSTPGDELKGGFDLDYGEKSKTNMSGYIEGPLGDIVSARINLSYSDKEGHYDNAVVE
                     GQRMGDEESMAVNALIDFHPSDNVNIRIRAGHYEDDDGPGAYAVAGGLAEHNYCAGVC
                     YWDVDAKNWSFYRGPVTLLESVFKGKISTPDSIGASTSAELFAKAMSHLTNADGSWTG
                     TNGANSTDGYYPIGSLDFNDLSHNGYGLAREGDRVSISISIDISDSMNLSMTYGDNED
                     EFLAFTDFDASGTFGFHTANARITTDESFEARLSGSNENFDWSVGFNEAEIMTKFHGG
                     FFDDQIFYNYWFADIKQPGIAAAMTSADTSGLFASIDYHVNDQISITAELRRQEDKVD
                     EPSVNVISPGLSPGSFKKTLPRVSIKNQLSDDAMTYFTYSEGNLPGGFNPEVASKLRN
                     AGQVTEFETQNPGIGQTFKEESLTNYEFGYKKTALDGRLAYNVALFHMERTDQVYSGF
                     GTITADGICNAGSETVPTCTVAFSGNGQSSDIDGFELDVTYLVSENLELRTSLAYVDA
                     SISKFPDGAGCGDVQVVFGNCDNVKGSEAPRYPKWQGSLIASYETASNLYIRGELFYN
                     GGYYDEVTNLAEIPSATEINLRLGKRMDNITAELYVTNLTDEDAPLAGNNIADTSGYV
                     RTNSYGYNFTSESVHLALRDKREFGVRFMYEF"
BASE COUNT         1116 a          813 c          603 g         1065 t
ORIGIN      
        1 agtcctttat ttattgacct agaaactctt gaataatctc agcgtgtagt tcaggctgct
       61 caaaaaactg ccagcttcca caattcggta tgttacgcaa aatgctgcct ggaattcttc
      121 gctgcaatgc agccacatca cctgcatata tatcttcttc agccttaagc agtagtgtct
      181 ctttattaaa ttcatcaaga tttggcgaat aacgataaac cgcataatgc aaatcatagc
      241 gcctttgtcg tagagtcaag ctactaagga aaggttgaaa cgttacctct ggaggagtat
      301 tgctggcgct catcttacga tacacagacc aagtcttttg aagaaaatcc ccatctacat
      361 ctataggtaa atcacgcgtc ataggtgtat tcaatttttt ttgtaattcc tcttcactca
      421 cattttcatc atgacctgca ccagatagaa ttacttttcc gactctttct gggtagcgta
      481 taccaagatt gatcaccact acagcacccc catgttgacc cagcatgtga gcttgggtaa
      541 tgcccatatt atctagtaca tcaatcacag catcagcata ttcatctact gtaagttcgt
      601 gatcagaagt atcagattga ccatggtttg gtaagtcgat gccaacaacc gtgaaagaat
      661 ctgccaacaa gggtgcaatt gctaaaaatt ctgtggacgc ttgtccagac tgatgcagca
      721 tcagcaatgt tgagccttga ccaatactcc agtggtgaac ccgtccaaac cgagtattag
      781 aatatgatcg ctggatatct ccagccatgc cgggcatggc tgcgttcacc ccaagatcaa
      841 acgaaccaat cagcgaagta ctgattagta aagccgaaaa caacaattgt cttctattca
      901 acagagacta accagctaaa aatttaacca tcatgcatta attataacag ttactagaag
      961 cgataattaa ccgttgctcc aagctaaaat tcatacataa aacgaacacc aaattctctt
     1021 ttatcacgca acgctaaatg cacactctca ctagtaaagt tatagccata actattggtt
     1081 cttacataac cactagtatc agcaatattg ttaccagcta atggtgcatc ttcatcggtt
     1141 aagttagtga catatagttc agctgttata ttatccatcc gcttacctaa cctaagatta
     1201 atctctgttg cacttggaat ttctgcaagg ttagtaacct catcataata accaccgttg
     1261 tagaacaact cacctctgat atagagattt gatgctgttt cataactggc aatcaatgag
     1321 ccttgccatt taggatatcg gggtgcttct gagcctttaa cgttatcaca attaccaaac
     1381 actacttgaa catctccaca acctgcacca tcaggaaact tactgatcga agcatcgaca
     1441 taagccaagc ttgttcttaa ctccaaattc tctgaaacta gataagttac atctaattca
     1501 aacccatcaa tgtctgaaga ttgaccatta ccgctgaagg caacagtaca agttggcaca
     1561 gtttcactac ctgcgttaca gatcccatct gctgttatcg taccaaatcc agaataaacc
     1621 tgatcagttc tttccatgtg gaacagagca acgttataag caagccttcc gtctaatgca
     1681 gttttcttat atccaaactc atagttagtt agtgattcct ctttaaaagt ctgtcctata
     1741 cctggatttt gtgtctcaaa ctcagtaact tgaccagcat ttctcaattt cgatgcaact
     1801 tcggggttga agccaccagg taagttacct tcgctgtaag taaagtaggt catagcatcg
     1861 tctgagagtt gattctttat agacacacga ggcaatgttt tcttaaagct gcctggtgat
     1921 aatccaggtg aaattacgtt aacagaaggt tcatcaacct tatcttcttg tcgacgcaat
     1981 tccgcagtaa tacttatttg atcattaaca tgataatcaa tagatgcaaa taaacctgag
     2041 gtatctgctg aggtcattgc tgcagcaatc cctggttgtt taatatctgc aaaccaataa
     2101 ttgtaaaaga tctgatcatc aaaaaatcca ccgtgaaatt tagtcattat ttcagcttca
     2161 ttgaaaccaa cagaccaatc aaaattctca ttgctgcctg ataatctagc ctcaaaagat
     2221 tcgtctgtag ttatccttgc attagcagta tgaaacccaa aagttccaga agcatcaaag
     2281 tctgtaaaag ctagaaattc atcttcatta tcgccataag tcatacttag attcatggaa
     2341 tctgaaatat caatagagat gctaattgaa actctgtcac cttctctagc caagccatat
     2401 ccattgtgag acagatcatt aaaatctaaa ctcccaatag gatagtaacc gtctgttgaa
     2461 tttgcaccat tggtgcctgt ccaagaccca tcagcattag ttaaatgact catagccttt
     2521 gcaaatagtt ctgcagacgt agaagcacct atagagtctg gtgttgatat tttaccctta
     2581 aagactgatt cgagaagtgt tactggacca cggtagaaag accaattttt tgcatcaaca
     2641 tcccagtagc acacaccagc acaataatta tgctcagcca aaccacctgc gactgcataa
     2701 gcgcccggac cgtcatcatc ttcataatgc cctgctctaa ttctaatgtt tacattatca
     2761 ctaggatgga aatcaattaa agcgttaacg gccatgcttt cttcatcgcc cattctttga
     2821 ccttcaacaa cagcattatc gtaatgccct tctttgtcac tatagctaag attgattcta
     2881 gcgctaacaa tgtcacctaa cggtccttct atgtagcctg acatattggt tttactcttc
     2941 tctccataat ctaaatcaaa accacctttt aattcatcac ctggagtaga tgtgatgatg
     3001 ttgatcgcac cagaatatgt gttccgtcca aagacagcag attgaggtcc tttaataatc
     3061 tcaacgcgtt ctgcattact aacaggaaga ctgtgaagcc caccggttac aagcacaccg
     3121 tcaataaaaa cactacctgt tctggccaac ggactagttg tatcaaaagt aacaccacgg
     3181 aaacgtggca tcgccattac ccttgcatta gtgttgttaa tagcttcaat aaaaaagcca
     3241 ggtgttgatt tttggatatc caaaatattt tgaaaagcag agtcctttat gtcttgtgca
     3301 ctcaatgcag ttactgctaa tggtgtttcc aatattgttt cttccttttt tcttgcagta
     3361 acaacaatct cttcaattcc ttcttgagag aaggtgacag ttggcatcaa ggcaattgtt
     3421 ataactacca tgatcgctga gttgactaaa aaactaaaac tttctcttat agtcatgaga
     3481 tattccccta agtatttaac ctattagcat gtatactaca taaaaagaca ggaggctgtc
     3541 aatttgacag acaagttaat attgtagaat atacaatctt gagaaagata ctaatac
//
LOCUS       DASCTH010000263         4218 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000558737, whole genome shotgun sequence.
ACCESSION   DASCTH010000263 DASCTH010000000
VERSION     DASCTH010000263.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000558737"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="QGH35_05545"
     CDS             <1..1180
                     /locus_tag="QGH35_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014798.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HJL82029.1"
                     /translation="ALNWEYDPFEIPEQIKNAWDASQRGATVETAWVDLLKAYTSDFP
                     EQGNDLESRLNPDFSSVRFDGLQELIEDLQTNDDPVATRKSSLIVLNALARDFPELLG
                     GSADLTGSNCTYHQTSKAITPDDTNGNYVFYGVREFGMAAIMNGMSLHGGMIPYGGTF
                     LTFADYSRNALRMAAMMELGVIHIFTHDSIGLGEDGPTHQPIEHIPSLRLIPGLNVWR
                     PCDTTETAVAWQSAIQCRTSPSALILTRQSLPQYQRSPEQLQNILCGGYILHQEENDS
                     LDAVIIATGSEIQLAMNAASKAAQNGHQVRVVSMPCLDIFELQPETYQYCVVPEGFEK
                     VMIIEASKASGWSQYRSPKGIVIGMDSFGKSAPAPELFEHFGFSDDNVYQQLMTMIEK
                     "
     gene            1195..2208
                     /gene="gap"
                     /locus_tag="QGH35_05550"
     CDS             1195..2208
                     /gene="gap"
                     /locus_tag="QGH35_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HJL82030.1"
                     /translation="MAVKIAINGYGRIGRNILKALFEGDKTNALEIVAINDLGDAESN
                     AYLTKYDTAHGIFPFDVEVNDDCLVIGGKQIQVFSERDPEKLPWQDLGVEVVLECTGF
                     FRTRELASKHLKAGAKKVVISAPAGTDIDATIVYGVNHHTLSKEHEIISNASCTTNCL
                     APLALPLHQAIGIESGIMTTIHAYTNDQVLTDVLHKDLRRARSATQSMIPTKTGAASA
                     VGLVLPELQGKLDGFSIRVPTINVSLVDLSFIASRPTMIEEINEVIKVAVSGPLKNVL
                     DYSDGPLVSVDFNHNPASSTYDATMTKVQEGTLVKVCSWYDNEWGFSNRMLDTTLALM
                     NAS"
     gene            2243..3397
                     /locus_tag="QGH35_05555"
     CDS             2243..3397
                     /locus_tag="QGH35_05555"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020562541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HJL82031.1"
                     /translation="MKDLDLQGQKTMLRLDLNVPIENGQITSDARIEAAIPTILAAQE
                     QGGKVIILSHLGRPDPGALKAIDSLQPVAEKLAQLSGQQVHFEPNWIESIPDIDNGIV
                     VCENVRFHEGETSNSEALSKQIASLCDVYVMDAFGTAHRKHATTYGVITFVQTVCAGP
                     LLTSEIESLSKAMQAPERPLVAIIGGAKVSSKLGVIETLAGICDHIIVGGGIANTFLA
                     AAGIETGNSLIEKDMIAMAGNILKSNKVNIPLPDDVTVAKKLSPESEAVKKSVVDIEK
                     DDQVLDIGPKTAEVYKSIIDSARTIIWNGPLGVFEIDQFSGGTEALALAVAENNSYTL
                     VGGGDTVAVLEKYGIKDSIDYISSGGGAFLEFIKSGTLLSLEVISRHTKS"
     gene            3409..>4218
                     /locus_tag="QGH35_05560"
     CDS             3409..>4218
                     /locus_tag="QGH35_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL82032.1"
                     /translation="MLLRFFALFTKATKVEAHEIKAVFLSFAFVFTILASYYILRPVR
                     DAMSSDWTDVELSTIWTFTFLISFIVVSLYGFACSKIKFKFLVPSVYGFFALTFLLLF
                     LLIQFFPDLNLINQIFYIWVSVFSLLNISVFWSFMADTYNKEQAKRLFGFIASGSSLG
                     AIFGPITALALAGVAGSESLILISASMLLIPIAIVFHLEKIRVAELQNTTASEFNEQP
                     MGSNILAGFKTFATTPVLLGIGLFIVLYSGISTFVYFELKNILASIDQDTRT"
BASE COUNT         1213 a          831 c          961 g         1213 t
ORIGIN      
        1 agcactcaac tgggaatacg atccatttga aattccggaa caaattaaaa atgcgtggga
       61 tgcatctcaa cgaggtgcta cagtcgaaac agcttgggtc gatttactca aggcatacac
      121 atcagatttt ccggaacaag gcaatgattt agagagtaga ctgaaccctg atttttcaag
      181 tgtaagattc gacggtctac aagaactgat tgaggatttg caaaccaatg atgatccagt
      241 tgcaaccaga aagtcttcct tgattgtatt aaatgcattg gcaagagatt ttccagaact
      301 ccttggaggt tctgctgatt taactggttc aaattgtact tatcatcaga cctcaaaagc
      361 cattactccg gatgatacga atggtaacta tgtgttttac ggggtcagag aatttggaat
      421 ggcagcgatc atgaatggta tgagtttgca tggaggtatg attccttatg gtggcacatt
      481 cttgactttt gcagattatt ctagaaatgc attgcgtatg gcagcgatga tggaactagg
      541 cgtgatccat atttttaccc atgactcaat tgggttgggc gaagacggac ccacccatca
      601 gcccattgaa cacatcccca gcctaagatt gatacctggg ttgaacgtat ggcgtccttg
      661 cgatacgacc gagaccgccg tggcttggca gtctgcgatt cagtgtagaa ctagtccttc
      721 cgcactgatc ctgaccagac agtcattgcc acaatatcaa agatctccag agcaactaca
      781 aaatatttta tgcggtggtt atattttaca tcaggaggag aatgattcat tggatgcagt
      841 cattatcgcc actggatcag aaatacagct tgccatgaat gctgccagta aagcggcaca
      901 aaatggtcat caggtcaggg tggtttccat gccatgtttg gatatttttg aactgcaacc
      961 ggagacatat cagtactgtg ttgtacctga aggttttgaa aaagtgatga ttattgaagc
     1021 ctctaaagcc agtggatgga gtcaatatcg aagcccgaaa gggatcgtca tcgggatgga
     1081 ttcttttggc aagtccgccc ctgcaccaga actatttgaa catttcggat tttcagatga
     1141 caatgtgtac caacaattaa tgactatgat tgaaaaataa atgaggagat tgggatggca
     1201 gtaaaaattg cgattaacgg ctacggcagg attggcagga atatcttaaa agctttgttt
     1261 gaaggtgaca agaccaatgc attggaaatt gtggcaatca atgatcttgg tgatgctgaa
     1321 agcaatgcct atctgaccaa gtatgatacg gctcatggta tctttccttt cgatgttgag
     1381 gttaatgacg attgtctagt gattggcggc aaacaaattc aagttttttc agaaagagat
     1441 cctgagaaac ttccatggca ggatttggga gtggaagtgg tactcgaatg caccggattt
     1501 tttagaacac gggaactggc aagcaaacat ctaaaagctg gggctaaaaa agttgtaatt
     1561 tcggcaccgg ctggtacaga cattgatgcc actattgttt atggtgtgaa tcaccatacg
     1621 ctctccaagg aacatgagat tatttccaat gcttcctgta ccaccaattg tcttgctcct
     1681 ttggcgttgc cactccatca agcgattgga atcgaaagtg gaattatgac caccatacat
     1741 gcatacacta acgatcaggt gctaaccgat gttctccaca aggatcttag aagagctcgt
     1801 tcagcaactc aatccatgat tccgaccaaa actggagccg catcagcagt aggtttagta
     1861 ttgccggaac ttcaaggaaa actcgatggc ttttcaatca gggtgcccac tatcaatgta
     1921 tccttggttg atcttagttt tattgcgtct agaccgacaa tgatcgagga gatcaatgag
     1981 gtgatcaaag tagctgtatc cggtccatta aaaaatgttt tggactacag cgatggtcct
     2041 ctggtttcag ttgatttcaa tcacaatcca gcttcatcga cttacgatgc aaccatgacc
     2101 aaagtccaag agggcacttt agtgaaagtt tgctcatggt atgacaatga atggggtttc
     2161 tccaaccgaa tgctggatac cacattggca ttaatgaacg ccagctaacc caatagcgag
     2221 ggtcgaaatg atattccctc aaatgaaaga ccttgatctt caggggcaaa agaccatgct
     2281 cagattggat cttaatgtac ccatagaaaa tggtcagatt accagtgatg cgagaataga
     2341 agctgcgatt ccaaccattt tggcagccca agagcaggga gggaaagtga ttattctatc
     2401 gcatttgggt agacctgatc caggggcgct gaaagcaatt gattccctac aaccagtagc
     2461 tgaaaagcta gcacagctaa gtggtcaaca agttcacttt gaacccaact ggattgaatc
     2521 aataccggat attgacaatg gtatcgttgt ctgtgagaac gtgagatttc atgaaggcga
     2581 aacatccaac agcgaggcat tatctaaaca aatagcatcc ttgtgtgatg tctatgtgat
     2641 ggatgctttt ggtacagccc acagaaaaca tgccactacc tatggagtta tcacttttgt
     2701 gcaaacagtg tgtgccggac ccttattgac ctcggaaatt gaatcattga gcaaagccat
     2761 gcaggcacct gagagacctt tagtggcaat tattggagga gccaaggttt catctaaact
     2821 gggagttatt gaaacactag ctggcatttg cgatcacatc attgttggtg ggggtattgc
     2881 caacactttt ttagctgctg ccgggataga gacgggtaat tcactgattg aaaaagacat
     2941 gattgcaatg gcgggaaata ttcttaagag taacaaagtc aatatacccc tccctgacga
     3001 tgttacggtt gcaaaaaaat tatctcctga gtctgaagca gtcaagaaaa gtgtggtcga
     3061 tatagaaaag gacgatcaag ttctcgacat cggtccaaag accgcagaag tatataagtc
     3121 tattatagac tctgctagaa ctatcatttg gaacggtccg ttgggcgtgt ttgaaataga
     3181 ccagttcagt ggaggtaccg aagcattggc actggctgta gcagagaata atagttatac
     3241 ccttgtcggt gggggtgata ctgttgcagt actagaaaaa tatgggatta aggattctat
     3301 cgattatatt tcaagcggtg gaggcgcctt tctagagttc attaaaagtg gcacactcct
     3361 ttcattggaa gtaatatcta ggcacactaa aagctaagga gaaataaaat gctactcaga
     3421 tttttcgctc tgtttaccaa agcaaccaag gttgaggccc atgagatcaa agccgtattc
     3481 ttgtccttcg cttttgtctt tactattttg gcatcctatt acattctccg tcctgtgcgt
     3541 gacgctatgt ccagtgactg gaccgatgta gaattgagta cgatctggac gtttactttc
     3601 ctcattagtt ttattgtggt atcactatat ggttttgcat gttcgaaaat aaaatttaag
     3661 ttcttggtcc ccagtgttta cggattcttt gcactaactt ttttactact atttctgttg
     3721 attcagttct ttcccgattt aaatctaatc aaccaaatat tctatatttg ggtcagtgtg
     3781 ttcagcctgc tcaatatttc tgtattttgg agctttatgg ccgataccta taataaggaa
     3841 caggcaaaaa gactcttcgg ttttattgca tctggcagca gtcttggggc catatttgga
     3901 ccgattactg ctttggcact tgccggagta gctggttccg aaagtttgat tttgatttcc
     3961 gcaagtatgt tactgatacc gattgctata gtttttcatc ttgaaaaaat aagagtagcg
     4021 gaactacaaa acactactgc ttccgagttt aatgagcaac caatgggttc gaatattctt
     4081 gcaggattca aaacctttgc cactacccct gtcctcttgg gcattggctt gtttatcgtt
     4141 ctctattctg ggattagtac ctttgtttat tttgagctga aaaatatctt ggcgagtatc
     4201 gatcaagata caaggact
//
LOCUS       DASCTH010000264         2575 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000570251, whole genome shotgun sequence.
ACCESSION   DASCTH010000264 DASCTH010000000
VERSION     DASCTH010000264.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000570251"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="QGH35_05565"
     CDS             <1..701
                     /locus_tag="QGH35_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024579.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82033.1"
                     /translation="KSIKPHENWFQLELAILFTNKQFDEAIEVLKKMATHWPEKEKYW
                     ETMAGVYMEIQRDPDALAALTLGYKNNAISKKKTLENLARLNLFLEIPYQAAVIVQEN
                     IDNGSLEKNEANLKLLLGSWTAAREFDAAIKVIDILAPMTNQGDLYIQKAMLLNEKGD
                     WEGVRTAAKQALETGTIDKPGDVYILLGMAYTELEEYEQAIDSFEKVLEVGAERNKKN
                     AEAWIEYVSDRQGS"
     gene            complement(703..1431)
                     /locus_tag="QGH35_05570"
     CDS             complement(703..1431)
                     /locus_tag="QGH35_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HJL82034.1"
                     /translation="MKTLLGYLFGAWAWFIFSVTTILMVLSFPFTRTVKTRRWIAHKG
                     MNWVLKILGYKIKTNGLDNLIDSPAILVANHASYIDPMVLAAVLPPNYAFVAKKELKY
                     IPFTGYVLSKLGTHLVDRFNAIKGAQDMRRIMTSSQMSDSIIFFPEGTFHAERGLSKF
                     KLGAFVTAIKNNLPVIPVAISGTRELLRSETWLPKRVSLKISVFPPIQCEDGVDQSKE
                     IAKQCRELILSAINEPDLKNKSFP"
     gene            1505..2419
                     /gene="xerD"
                     /locus_tag="QGH35_05575"
     CDS             1505..2419
                     /gene="xerD"
                     /locus_tag="QGH35_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HJL82035.1"
                     /translation="MTNSTSKSTSKSSDIDIFIDAIWMEGGLAKNTLSAYRSDLSTLE
                     KWAKNKKLSLKKISRADLLDFISERAKSGSSSRSSARQLSTYRRFYNYLAQQEIIVHN
                     PTLKISMPKIGRPLPKIISEEDVVKLIKAPIAKKPLGCRDRTMLELLYATGLRVSELV
                     QLKQTQINLNQGFIRVVGKGNKERLVPFGGFAERWLRRYLKGPIHEILGERRTDYLFP
                     TKTSECISRQAFWQLIKKYAKKSGLTSDLSPHTLRHAFATHLINHDADLRVVQMLLGH
                     SDLSTTQIYTHIAQQRLKDLHEKHHPRG"
BASE COUNT          878 a          464 c          502 g          731 t
ORIGIN      
        1 tgaaatcaat caaaccacat gagaactggt ttcaattgga actggcaatt ttgttcacaa
       61 ataagcaatt tgatgaggcg attgaagtgc ttaagaagat ggccacacat tggccagaaa
      121 aagagaaata ttgggaaacc atggccgggg tttatatgga gattcaaaga gacccagatg
      181 cgcttgcagc attaactttg gggtataaaa ataatgcaat tagcaagaaa aaaacgttag
      241 aaaacttagc ccgattaaat ctctttctag aaattccata ccaagctgca gtcattgttc
      301 aagaaaatat cgataatggt tctctggaaa aaaatgaggc taatttaaag ctattattag
      361 gttcgtggac agctgcaaga gaatttgatg cagccattaa agtcattgac attctggctc
      421 ccatgactaa tcaaggagat ttatacattc aaaaagcaat gcttttgaat gaaaaaggtg
      481 attgggaggg cgtgagaacg gcagcaaaac aggcactgga gacagggaca atagacaagc
      541 ccggggatgt ttacattctc ttgggaatgg cttataccga attggaagaa tacgaacagg
      601 cgatagactc atttgagaag gttttagaag tcggagcaga aagaaataaa aaaaatgccg
      661 aggcttggat agaatatgtc agcgacaggc aaggcagctg aactaaggaa acgatttatt
      721 tttcaaatcg ggctcattaa ttgcacttaa gatcagttca cggcattgtt tcgcgatttc
      781 tttagattga tcaactccat cctcacattg aatgggtgga aatacagaaa tcttaagtga
      841 tactcttttt ggcaaccaag tttctgatcg aagaagttca cgtgttccac taatcgcaac
      901 tggaataaca ggtaagttat tttttattgc agtgacaaat gcacccagtt taaatttgct
      961 taaacccctt tctgcatgaa atgttccctc aggaaaaaaa ataattgaat ctgacatttg
     1021 ggagctcgtc ataatccgtc tcatatcttg ggcacccttt attgcattaa agcggtccac
     1081 taaatgcgtt cctaatttac ttaatacata accagtgaaa ggaatgtact tcaattcttt
     1141 tttcgccaca aaagcatagt tgggtggcaa tactgcagcc aataccattg ggtcgatata
     1201 gctagcatga ttcgcaacaa ggatagcagg tgagtcaatc agattgtcta atccattagt
     1261 ttttatttta taacccagta tttttagtac ccaattcatt cctttgtgag cgatccaccg
     1321 tctagttttt actgtgcgtg tgaacgggaa gctcaagacc atgagaatag tagtcactga
     1381 aaaaataaac caagcccaag ccccaaaaag atatcccaat agtgttttca ttgtaatttt
     1441 tttggatttt ttataataaa acgtcctatt cttataggct atgatacaca atcaacgata
     1501 aacaatgaca aattcaactt ctaaatccac ctctaaatct tccgatatag atatctttat
     1561 tgatgccata tggatggaag gaggcttagc gaaaaatact ttgtctgcat atcgatcaga
     1621 tttatcaact cttgagaaat gggctaaaaa taaaaaatta tcacttaaga agatctcaag
     1681 ggctgactta ttagatttta tatcggagcg agccaaatcg ggttcaagtt cacgatcctc
     1741 ggcaagacag ttgtctactt acagacggtt ttacaactat cttgctcaac aagaaatcat
     1801 tgttcacaat ccaaccttaa aaatttcaat gccaaaaata ggcagaccat tgcccaaaat
     1861 aatctctgaa gaagatgtgg taaaactcat taaagcacct atagctaaga aacctttggg
     1921 gtgtagggat agaaccatgc ttgaactact ctatgctacg ggactgaggg tttcagaatt
     1981 ggttcaattg aaacagactc aaataaatct caatcaaggc tttataaggg ttgttggaaa
     2041 aggtaataaa gaaaggttgg tgccatttgg aggctttgca gaaagatggc tcagacgtta
     2101 tctcaagggc cccattcatg aaattctcgg agagcgaaga acagactatc ttttcccaac
     2161 gaaaacctct gaatgtattt ctaggcaagc tttttggcaa ttgattaaga aatatgcaaa
     2221 aaaatctggt ttaacatcgg atttgtctcc acatactttg agacacgctt ttgcgacaca
     2281 tttaattaat catgacgcag atttaagagt ggtacaaatg ttgctaggac acagtgattt
     2341 atcaaccacc cagatctata cacatattgc gcaacaaaga ctcaaagatc tacatgaaaa
     2401 acatcatcct cggggttgat ttttttgtac actagggctt atgaaagaga ttcttataaa
     2461 aacagttata acttgtattc tgttattcag taacgtccta ttgtcccaag atggaattga
     2521 catcaaagat tctgaagctt ctcaaactaa tgaatttgaa gagtataaaa aacaa
//
LOCUS       DASCTH010000265         5860 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000572134, whole genome shotgun sequence.
ACCESSION   DASCTH010000265 DASCTH010000000
VERSION     DASCTH010000265.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000572134"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="QGH35_05580"
     CDS             complement(<1..852)
                     /locus_tag="QGH35_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HJL82036.1"
                     /translation="MSITRRELIAALGTLAATSNLYADQSSQQQSRQLLKGLACNMES
                     WWTDLDFMQRFERAAQSGFSSIEFWEHNKNKRNMNAVAALARKLDLNIVQFTGWGGPS
                     LADTSNHNDFIETMKRVTEIAHRLDAPMFTVVGHQTVKTIPQAASLANLSLALETAAP
                     ILEDAKKMLILEPFNPVDHQGHFLNGSVDALRICREIDSPFIKINWDLYHMQLTEGNI
                     VENLYAGIDQVGYIQVADIPGRHQPGSGELNYNFIFNTIDDIGYKGPIGLECWPENND
                     EKQAIADI"
     gene            1032..2198
                     /locus_tag="QGH35_05585"
     CDS             1032..2198
                     /locus_tag="QGH35_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010136464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL82037.1"
                     /translation="MSIFNKNRLFIASCTSLALTAMTFAIRARLETVFGPTGVGLTLE
                     QIGYAFMPAFWGFTLAMIVGGPIVDYFGMKKVMWLAFILHAFGIIFTLAATDFSSLFT
                     ATILMGLGNGMIEAVCNPLVASMYPTQKTKMLNRFHLWWPAGIVIGSIIGYLIMDILG
                     LGWQVMVASLFIPLVVYGYLITGQPFPLTERVEMGISTKVALKSLLTPLYLFIAFCML
                     LSATTELGTTQRIESLLKETGINALLVLAFINGLMMIGRYFAGPISKKINTTGMLWFS
                     ALFSFIGLQLLANASGMFIFFAAGVFAIGITFFWPTTLAFISENIPESGAFGLSIMGG
                     LGNLSVSIVLPIMGRFLDNSNGMQVIQTMSTLPAILFVLYGALYYYRKQYTQTR"
     gene            complement(2222..3379)
                     /locus_tag="QGH35_05590"
     CDS             complement(2222..3379)
                     /locus_tag="QGH35_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HJL82038.1"
                     /translation="MADIEKKDPIRWGMVGGGQGSFIGEVHRIAARLNNNYLFCSGAL
                     SSTPERSQISGSEAGLEKDRSYPNYEAMIKGESSRDDRIEAVSIVTPNDTHFPIAKRF
                     LQEGFHVICDKPMTVTTHEADELVQLANQRNLIFAVTYNYSGHPMVRHARAMIKNGDI
                     GAIRVLQCEYAQDWLAESIEKEGHKQALWRTDPLQSGLGGCIGDIGTHAFHLACFVSG
                     LEPEALCADISIFGDGRKLDDNANVLLRFKGNARGIIWVSQVAIGNENNLKLRIFGEQ
                     GGLSWRQEEPNELIFSPLNQPSRKISGDSSKASDEASIITKVPAGHPEGYLEGFATIY
                     SEIAQAIFAARSGETVDQNIIFPTAEDGKAGVNFIEAVVRSSNKGSGWVNL"
     gene            complement(3393..4448)
                     /locus_tag="QGH35_05595"
     CDS             complement(3393..4448)
                     /locus_tag="QGH35_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HJL82039.1"
                     /translation="MKTIKGPAIFLAQFVGDEPPFNTLDNIAKWAAGLGFKGVQIPSW
                     DSRLFDLEKAANSQSYCTEIHEILDNHGLVITELSTHTQGQLVAVHPAYDLLFDSFAV
                     PEVRGNPELRQQWAVSQMLLAAKASENLGLTEHATFSGALAWPYLYPWPQRPAGLITT
                     AFDELAKRWLPILNAFDDAGINLCYEIHPGEDLHDGSTFEMFLERVDNHNRCQMLYDP
                     SHYMLQALDYLQHIDIYHERIKIFHVKDAELNPTGRQGVYGGYQSWLDRAGRFRSLGD
                     GQIDFNSIFSKFAQYDFQGWAVLEWECCLKHPEDGAKEGAAFIRDQIIRVTDKAFDDF
                     AASDSDKKSNKSLLGLP"
     gene            complement(4614..>5860)
                     /gene="dacB"
                     /locus_tag="QGH35_05600"
     CDS             complement(4614..>5860)
                     /gene="dacB"
                     /locus_tag="QGH35_05600"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HJL82040.1"
                     /translation="LRSPASLTKLFTTFIALDYLGPDFQWNTNIYVSDSISDETVDYL
                     LFKGEGDPYLTEENLRSIVKKLRHLGLERIEMGLQVDQQYFEANQSHPGDFDDDPLRP
                     YNQLPSALTANFNRVDFQLVPNVKTGSVDFYFDTLPSGVVIDNQLHLGKGQCSDFTDS
                     VVFHETRTDQIMTIIAEGVFPSSCLKIEHEIALTNTNHYFYSLFSDLWKQSGGEFLGY
                     IIDSEKIGESEVPLLVHTSPPLNEIIRHTNKQSNNFMSRQIFLSLGNYQSDQTATLEL
                     SRLRINAMLDKYDIDFQGQFIDNGSGLSRETVIRGETLMQLLKEIYEHPLRAELISSL
                     PITGIDGTMKKRFRKFPIKQRAHLKTGTLDGVTGMAGYVKGLSGKDYIVVFIHNDFSE
                     HSYRVKEFQKALLTWIIQDYKW"
BASE COUNT         1719 a         1225 c         1058 g         1858 t
ORIGIN      
        1 aatatctgct atagcttgct tttcatcatt attttctggc caacattcta gtccaatcgg
       61 tcctttatag cctatgtcat cgatggtatt gaaaataaaa ttataattca attctcccga
      121 cccaggttga tggcgtcctg gaatatcagc gacttgtata taaccaactt ggtctattcc
      181 agcataaaga ttttccacta tattgccttc ggttaattgc atgtggtaca ggtcccagtt
      241 aattttaata aaaggcgaat caatttccct gcagattcgt aacgcatcca ctgacccatt
      301 taaaaaatgc ccctgatggt caaccggatt aaaaggctcc agtattaaca tcttttttgc
      361 atcctccaaa ataggagctg ctgtttccag agctagggac aaatttgcca agcttgctgc
      421 ttgcgggatg gttttcactg tttggtgtcc aaccacggta aacattgggg catctaatcg
      481 atgagctatt tcagtaactc gtttcattgt ttcaataaaa tcattgtgat tactggtatc
      541 agcaagactg gggccgcccc agcctgtgaa ttgaacaata ttgagatcca gttttctggc
      601 caatgcggca acagcattca tgtttctttt atttttgttg tgttcccaaa attctattga
      661 actgaatcct gactgtgcag ccctctcaaa tcgctgcata aaatcaagat cagtccacca
      721 agattccatg ttacaagcca agccttttaa caattgccga gattgttgtt gagaagattg
      781 atctgcataa agattagagg tggctgctag tgttcctaat gctgcaatta attctcttcg
      841 tgttatagac attttaaaat cacactgtat aaacctcaga tgaaccatag ccaatggttc
      901 agtttgtggc aatcaaattt ttagctaata tcttaaaact tcttttaaaa gatttaaata
      961 agtaggatta atacccatca tttgagaaac tcttataact cactttaata caatgaagat
     1021 tcgataataa tatgagcata tttaataaaa accgtctttt tattgccagt tgcacctcct
     1081 tggctctaac cgcaatgacc tttgcgattc gtgcacgatt agaaacagtt tttggaccga
     1141 caggggtcgg tttgacattg gagcaaatag gctacgcttt tatgcctgca ttttggggat
     1201 ttactctagc gatgattgtt ggtggaccta tcgtcgatta tttcggtatg aaaaaagtta
     1261 tgtggctagc ttttattcta catgcattcg gaattatctt tacattagct gctacggatt
     1321 tttcatcact atttacagca accatcctca tgggattggg taatggtatg attgaagctg
     1381 tctgcaaccc tttagtggca agtatgtatc ccactcaaaa aactaaaatg ctcaatcgtt
     1441 ttcatttatg gtggcctgca ggaattgtta ttggctcaat cattggatac ttaatcatgg
     1501 atatcctagg tctgggctgg caagtgatgg tggcatctct ttttatccct ctagttgtgt
     1561 atggttacct aattacaggt caaccatttc cattaactga acgggtagaa atggggatca
     1621 gtacaaaggt agctctcaaa agtctcttga cgccacttta tctatttatt gccttttgta
     1681 tgttgctttc ggctacaacg gaattaggaa caactcaacg catcgaatca ctgctaaaag
     1741 aaacaggaat caatgcactc cttgtactcg catttattaa tggtcttatg atgattggtc
     1801 gatattttgc tggtccaatt tcaaaaaaaa ttaatacgac tggtatgttg tggttttcag
     1861 cattattttc gtttattgga ttgcaacttc ttgctaacgc ttcgggtatg tttatttttt
     1921 ttgctgccgg tgtttttgcc ataggaatca cttttttctg gccaactaca cttgcattta
     1981 tttcagaaaa tatacctgaa agtggtgctt ttgggctttc aattatggga gggcttggga
     2041 acttgtcggt ctcaatcgtg ctgccaataa tggggcgatt tcttgataat tcaaacggaa
     2101 tgcaagtcat ccaaacgatg agcacactgc cagctatact gtttgttttg tatggcgcac
     2161 tatattatta tcgaaaacaa tacacacaaa cccgttaagt gaaagcaaaa actgcttggc
     2221 cttataaatt tacccaaccc gaacctttgt tgcttgaacg aacgaccgct tctataaaat
     2281 tcactccagc tttaccgtcc tcagctgtag gaaaaattat attttgatct acagtctcac
     2341 cactgcgcgc agcaaaaatt gcctgtgcaa tttcactata aattgttgca aaaccctcta
     2401 aatatccttc aggatgacct gcagggactt tggttatgat cgatgcctca tcagaggctt
     2461 ttgagctgtc tccagagatt tttcgtgatg gctgatttaa aggagaaaaa attaattcat
     2521 taggctcttc ttgccgccaa ctcagtccac cttgctcacc aaaaattcta agttttaaat
     2581 tattttcatt accaatcgcc acttgactca cccaaataat acctctggca tttcctttaa
     2641 atcgtaataa aacgttggcg ttatcgtcta actttcgtcc atcgccaaat attgaaatat
     2701 cggcacacaa agcctctggc tctaatccac ttacaaaaca agctaaatga aatgcatgtg
     2761 tcccaatatc accaatacaa ccacccaatc ctgattgtaa aggatcagtt cgccacaaag
     2821 cttgtttgtg gccttctttt tcaattgact ctgctagcca atcctgagca tattcacatt
     2881 gtaagaccct aattgctcca atatcgccat tcttaatcat agctcgtgca tgcctgacca
     2941 ttggatgacc gctgtaatta taagtaacgg caaaaattaa gttacgctgg ttagcaagtt
     3001 gaactaattc atcagcttca tgagtggtta ctgtcattgg cttatcacaa atgacatgaa
     3061 aaccttcttg gagaaatctt tttgcaatgg gaaaatgcgt atcattgggc gtaacaattg
     3121 ataccgcttc aattcgatca tctcggctcg attcaccttt aatcattgcc tcatagtttg
     3181 gatacgatct atctttttcc aagcctgctt ctgaacctga aatttgagac cgttctggtg
     3241 tggacgataa ggcacctgaa caaaagagat agttgttatt gagacgggca gcgatacgat
     3301 gtacttcacc aatgaaggac ccctgacctc ctccgaccat tccccatcgg attggatctt
     3361 ttttttctat atcagccatt cagccatgtt gtttaaggca agcccagtaa acttttatta
     3421 gattttttat cgctatctga tgctgcgaaa tcatcgaaag ctttatctgt gacacgaata
     3481 atttgatcac gaataaatgc tgcgccttct tttgcaccat cttcaggatg ttttaaacaa
     3541 cattcccatt ctagaacagc ccacccttga aaatcgtatt gagcaaactt agaaaaaatg
     3601 ctattgaaat caatttgacc atcacccaaa gagcgaaatc gacctgctct atcgagccat
     3661 gactgataac ctccatagac accttgacgt cctgttgggt tcaactctgc atcttttacg
     3721 tgaaatattt tgatccgctc gtgataaata tcaatgtgct gaagataatc cagtgcttgt
     3781 aacatatagt gacttggatc gtatagcatc tgacatcgat tatggttatc aacacgctct
     3841 aaaaacattt caaatgtaga accatcgtgt aaatcctcac ccggatgtat ctcataacac
     3901 agattaattc cagcatcatc aaaggcattg agtatgggta accaccgctt ggctaattca
     3961 tcaaaagcag ttgttatgag tcctgctggg cgttgaggcc agggatacaa gtatggccaa
     4021 gccaaggcac cggaaaaagt ggcatgttca gttaagccta gattctcaga tgcttttgct
     4081 gcaagtagca tttgactcac tgcccattgt tgcctcaatt ctggattacc tcgaacttca
     4141 ggaactgcaa atgagtcaaa aagaagatca tacgcaggat gaacggctac cagttgcccc
     4201 tgggtatgtg ttgataactc tgtaatcacc aaaccatgat tatctaatat ttcatgaatt
     4261 tcagtacaat atgactggct atttgctgct ttttcaagat caaagagacg actatcccaa
     4321 cttggaattt gcacaccttt aaatccaaga cctgcagccc attttgctat gttatctaga
     4381 gtattaaatg gaggttcatc tccaacaaat tgagccagaa atatagctgg tcctttaatt
     4441 gttttcatca cactattcca aattcttgtt agtgtatgga ttcataatca tcataactaa
     4501 cttcataccc attattatac tcacaaacta aaacatagcc tccggcacta gtcatgcaaa
     4561 aaaatgggga attatcaaaa aaacactaag gttgcattcc gatttttgat atttcaccac
     4621 ttatagtctt gaataatcca tgtcaaaagg gctttttgaa actctttgac tctataggaa
     4681 tgttccgaaa aatcattatg aatgaaaaca acaatataat cctttcctga aagccctttt
     4741 acatatcctg ccataccagt cacaccatcc aaagtgccgg tttttaaatg ggctctttgt
     4801 ttaatgggga atttacgaaa ccgttttttc attgtgccgt caatgcctgt aatgggaaga
     4861 gaggagatca actcagctct caatggatgc tcatagatct cctttagtaa ttgcatcaaa
     4921 gtttcacctc taataacagt ttctctagaa agacctgagc cgttgtcaat aaattgacct
     4981 tggaagtcaa tgtcatattt atctaacatc gcattaattc ttaaacgaga cagttctaat
     5041 gttgccgttt gatcagactg gtaattgcca agggaaagaa atatctgacg actcataaaa
     5101 ttattactct gtttattggt gtgtcgaata atttcattca aaggaggtga tgtgtggacc
     5161 aataatggta cttcggattc gccaattttt tcactgtcta taatataacc aaggaactct
     5221 cctccacttt gtttccagag atcagagaat aagctgtaga aataatgatt agtattcgtc
     5281 aaagcgattt catgctcaat ttttaagcaa ctacttggaa acacgccctc agcaataatg
     5341 gtcattattt gatcggttcg tgtttcatga aatacaactg aatctgtaaa atctgagcat
     5401 tgacctttgc caagatgtaa ttggttatca atcactacac cactgggtag ggtatcaaaa
     5461 taaaaatcca cagatcctgt tttgacgttt ggaactaatt gaaaatcaac tctattaaaa
     5521 ttcgccgtca atgccgatgg tagttgattg taaggtcgca agggatcatc atcaaaatct
     5581 cctgggtggg attgattggc ctcaaaatat tgctggtcaa cctgaaggcc catttcaatc
     5641 ctctcaaggc ccaaatgtct gagtttttta actatggacc taagattctc ttcggtcaga
     5701 tatggatctc cttctccttt aaacaataag tagtctactg tctcatcaga tattgaatcg
     5761 gaaacataaa tattagtatt ccattgaaaa tcagggccca gataatccaa agcaataaac
     5821 gttgtgaaca actttgtcaa agatgctgga cttcttaaga
//
LOCUS       DASCTH010000266         1601 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000578050, whole genome shotgun sequence.
ACCESSION   DASCTH010000266 DASCTH010000000
VERSION     DASCTH010000266.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000578050"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="QGH35_05605"
     CDS             <1..1214
                     /locus_tag="QGH35_05605"
                     /inference="COORDINATES: protein motif:HMM:NF019604.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJL82041.1"
                     /translation="PYNGFVKQLTSKPVVGVGWFTSPDHMVSQINRGILDFVGSARAS
                     IADPFLPNKIFEGNEDDIRECIGCNICASCYNQGIPVRCTQNPTMGEEWRRGWHPERI
                     KKKTSDENILIIGGGPAGLEAAMSLGKRGYEVTLAEKDMELGGRINKEIQLPGMSSYS
                     RIRDYRTHQINQLENVSVFLDNQMKVEDVIDMEFEHVVIASGSSWELTTIDDKAIPIN
                     TNENSNFLTPDDILNGAKVNSPVVIYDFDYYYMGGLIAEFLSGQGHTVNMVTPFENIS
                     PWSFMSNELNEIKLRLNEKNVTIHTDSRVLSSNNSSIEIINCFNKDQSSLEASTVVIV
                     GTRKPRDEIYHALIKAKSPKIKSVTKIGDADAPGALYHAVYSGHLFAEYFGEEQQIDN
                     MKHEYPVIQPF"
     gene            1238..>1601
                     /locus_tag="QGH35_05610"
     CDS             1238..>1601
                     /locus_tag="QGH35_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82042.1"
                     /translation="MSKKTKESRREFLSYGASLLAGFPILANADPSKTIKKDIPKLVG
                     NSGTYDTVALKQEAVNLCLVQSEVTPVDGKNPKPGIKSNLDHMLFLIDAAQGYGGHKD
                     YLAFHEFPITGWDQWSREE"
BASE COUNT          543 a          291 c          329 g          438 t
ORIGIN      
        1 aaccttataa cggctttgta aaacaattaa cctcaaaacc agtcgttggt gtaggttggt
       61 tcacatcacc ggatcacatg gttagtcaaa tcaacagagg aattcttgat tttgtaggtt
      121 ctgctagagc ctctatagcc gacccatttc ttcctaataa aatctttgaa ggtaatgaag
      181 atgatattcg agaatgtatt ggctgcaaca tctgtgcctc ctgttacaac caagggattc
      241 ctgttagatg cacacagaac ccaacaatgg gagaagaatg gagaagaggg tggcaccctg
      301 agaggataaa gaaaaaaacc tctgatgaaa acattcttat cattggcggc ggacccgcag
      361 gactggaagc agctatgagt ctaggtaaac gaggttatga agtcactctt gcagagaaag
      421 atatggagct gggtggcaga atcaataagg aaatacaact tcctggtatg tcatcttatt
      481 caagaataag ggattatcga actcatcaaa ttaatcaatt agagaatgtg agtgtatttc
      541 tcgataatca aatgaaagta gaagatgtta ttgatatgga atttgaacac gtggtcattg
      601 catccggctc atcttgggaa ttaacaacca ttgatgataa agcgatccct attaacacta
      661 atgaaaacag caatttttta acccctgatg atattttgaa tggtgctaaa gtgaacagtc
      721 ctgtggtcat ttatgatttt gactattact acatgggagg cttaattgct gagttccttt
      781 caggacaagg tcatacagta aatatggtca caccatttga aaatatttcc ccatggagtt
      841 tcatgagtaa tgaattaaat gaaattaaac tcaggttaaa cgagaagaat gtaactattc
      901 atactgatag tcgggtgtta tccagtaaca acagttctat tgaaattatt aattgtttta
      961 acaaagatca atcatctctt gaggcatcaa cagtagtcat tgttggcaca cgtaaaccca
     1021 gagatgaaat ttatcatgca ttaataaaag caaagagtcc taaaatcaaa tccgttacta
     1081 agattggtga tgctgatgca ccgggtgctc tatatcatgc cgtttattca ggtcatttgt
     1141 ttgcagaata ttttggtgaa gagcaacaaa tagacaatat gaaacatgag tatcccgtga
     1201 tacaaccctt ttgatataaa cttagttagg taaaaccatg agcaagaaaa ccaaagaaag
     1261 ccgaagagaa tttctcagtt atggagcatc cttattagcc ggtttcccca tactagcaaa
     1321 tgcagaccca tcaaagacaa taaagaaaga tattccaaaa cttgtcggaa actcaggcac
     1381 ctacgatacc gttgccctaa aacaagaagc agtcaacctg tgtctcgtgc aaagtgaggt
     1441 aacccctgtg gatgggaaga acccaaaacc tggaattaaa tcaaacctgg atcacatgtt
     1501 atttcttatt gatgctgccc aaggctacgg cggccataaa gattacttag cctttcatga
     1561 atttccaatt acaggttggg accaatggag tagggaagag a
//
LOCUS       DASCTH010000267        15323 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000578474, whole genome shotgun sequence.
ACCESSION   DASCTH010000267 DASCTH010000000
VERSION     DASCTH010000267.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 15323)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15323)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15323
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000578474"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /gene="fusA"
                     /locus_tag="QGH35_05615"
     CDS             complement(<1..1622)
                     /gene="fusA"
                     /locus_tag="QGH35_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HJL82043.1"
                     /translation="MARNTPIDRYRNIGIMAHIDAGKTTTTERVLFYTGISHKIGEVH
                     DGNAVMDWMEQEQERGITITSAATTCFWQGMDKQFDQHRINIIDTPGHVDFTIEVERS
                     LRVLDSAVAVFCAVGGVEPQSETVWRQANKYSVPRLAFINKMDRAGADFLRVVEQIEQ
                     RLRAHPIPIQLPIGSEDSFSGVVDLIRMKAIIWNDEDLGVTYSEEEIPENMMEACKQW
                     HEKILESAAETSEKLLEHYLETGTLDSKQIISGLRARTLNGEIVPVICGSAFKNKGVQ
                     AMLDAIIHFLPSPIDKPPIYGILEDGTEGLRKADDDENFSALAFKIANDPYVGNLTFF
                     RVYSGVLNSGDTIYNSLRHQKERVGRILQMHSNSREEIKEVRAGDIAAAVGLKNVTTG
                     DTLADLKNIITLEKMEFPEPVISVAVEPKSTEDESKMGIGLQKLSKEDPSFQVKTDEE
                     SGQTIISGMGELHLDIIVDRLSREFNVEANVGKPQVAYRETIKKLVEQEAKFVRQTGG
                     RGQYGHVYLRIEPQEPGTGYEFVNAIKGGAIP"
     gene            complement(1648..2127)
                     /gene="rpsG"
                     /locus_tag="QGH35_05620"
     CDS             complement(1648..2127)
                     /gene="rpsG"
                     /locus_tag="QGH35_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HJL82044.1"
                     /translation="MSRRTRSSKREILPDPKYSSSLLAKFINIVMIDGKKSVAERIVY
                     DALETLIEKLGEPPERAPDLFAEVLDNIKPVVEVRSRRVGGATYQIPVEVRQDRREAL
                     AMRWLMEAARSRNENNMKSKLANELLDASKNRGNAIKKKEETHRMAEANKAFSHYRW"
     gene            complement(2134..2508)
                     /gene="rpsL"
                     /locus_tag="QGH35_05625"
     CDS             complement(2134..2508)
                     /gene="rpsL"
                     /locus_tag="QGH35_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009638963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HJL82045.1"
                     /translation="MTTINQLVRKPRKSKTYKNNVPALEGKPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGYEVTSYIGGEGHNLQEHSIVLIRGGRVKDLPGVRYHTIRGTL
                     DCSGVGDRKQGRSKYGTKKPKN"
     gene            complement(2584..6783)
                     /gene="rpoC"
                     /locus_tag="QGH35_05630"
     CDS             complement(2584..6783)
                     /gene="rpoC"
                     /locus_tag="QGH35_05630"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HJL82046.1"
                     /translation="MKNLLNFFKQKNQTEEFDYIKIGLASPEMIRSWSYGEVKKPETI
                     NYRTFRPERDGLFCAKIFGPVKDYECLCGKYKRLKHRGVVCDKCGVEVTQSKVRRERM
                     GHLELASPVAHIWFLKSLPSRVGLLLDMTLREIERVLYFEAYLVVEPGMTTLEMGQLL
                     SEEEYIEALGEFGNDFEAQMGAEAIHGVLKDIKLQDQLQKIHEDMNATASQIKLKRLS
                     KRLKLVESLIKSGNRPEWMILKNLPILPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELNAPEIIVRNEKRMLQKSVDALLDNGRMGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLKLHECGIPKKMALELFKPFVFSKLIYQGEAS
                     TIKAAKKLVELQGPEIWDILDNVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCKAFNADFDGDQMAVHVPLSIEAQVESRALMMSSNNILSPANGEPIIVPSQDIVL
                     GLYYMTREKVNALGEGQIYADITELIRAHESGHVGIHAKVQVRIPTNNDSKEEVEANY
                     ELVQTTVGRAILSTIVPKELPFSLINKTMDSKQISSLINKCYRDVGLKAAVVFADKLM
                     YMGFQHATQAAISISLDDMVVPELKDKILSAAEEQVRNIQNQFSSGLLTQGERYNKVV
                     DIWSRTNELISKAMMDTLGSEEVENQAGEKFTQPSFNSIFMMADSGARGSAAQIRQLA
                     GMRGLMAKPDGSIIETPITANFREGLNVFQYFISTHGARKGLADTALKTANSGYLTRR
                     LVDVSQDLVVSEEDCETSQGIIISAIIEGGEIVQKLGDRILGRIIAEPLLDKDNKKIL
                     KVGELIDEKNVELFELHGIERIQVRSPVTCETKHGVCSQCYGRDLARGNIVNKGEAVG
                     IIAAQSIGEPGTQLTMRTFHIGGAASSSAAANSIDVKTDGKVHLHNLKTISNTDKNLV
                     AISRSGEISITDNHGKEKERHKIPYGAVITVREGRKVKSGQVISTWDPHTHPIIAEAA
                     GVIKFENFIDGVTVTEQIDEMTGIDSIIIMDSKKQFGTSKILRPGAKLEKANGKPVLF
                     SGTETPMTYAFPPGAIMGVKDGAKIHSGDVIARIPLETSKTSDITGGLPRVADLFEAR
                     KPKGAAILAKYTGITSFGKETKGKVRLVITDEDGNSHEELILKSRRLNIFEGETIAKG
                     EVIADGEVSLHDIIEVQGIQELSEYLVKEVQDVYRLQGVPINDKHIEVIIRQMMRTVE
                     VDNPGDTQLLVNEQINKSDLLEINEKMIAEDLKPASFKPILMGITKAALATTSFISAA
                     SFQETTRVLTEAAVKGSKDKLRGLKENVVVGRLVPAGTGFKSEIDKASLAEEEFSKGL
                     RDLDTPTDHKDDQDLNQTKAQ"
     gene            complement(6802..10923)
                     /gene="rpoB"
                     /locus_tag="QGH35_05635"
     CDS             complement(6802..10923)
                     /gene="rpoB"
                     /locus_tag="QGH35_05635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HJL82047.1"
                     /translation="MAYSFTEKKRIRKDFGKQQSVLDVPDLLSIQLASYNKFLQRNVD
                     PAKRKNLGLEAAFNTLFPIESFSKNARLEFVSYRLDDPGFSVKECQLRGLSYASPIRV
                     KTKLITYNKETGEETVKEVKNIKESGDTSDVYFGEIPLMTDYGTFVINGTDRVIVSQL
                     HRSPGVFFDHDKGVQSSGKLLFSARIIPYRGSWLDFEFDAKDAIFARIDRRRKISVTI
                     MLHALGLSNEEILDIFFEKDKFSLSKKEIKLHFKPDHFKGITADFDIKIGRKVIIKKG
                     QRINGGHLRRLEELSKKNKFLTVPVDHLEGKVIAHDVVEPTTGELLALTNEILSIEKI
                     NILIENGVKAIETIHINDLDHGDYISNSMRIDPSSTKQEALTEIYRMMRPGEPPTKEA
                     SENLFQNLFFNEDRYDLSEVGRMKFNRRIGRKSDLGNNSLNNEDIIDVLKTLINIRNG
                     KDTVDDIDHLGNRRIRTVGEMTENAFRVGLVRVERAVKDRLSLVETEELMPRDLINSK
                     PVSAAIKEFFGSSQLSQFMDQNNPLSEVSHKRRISALGPGGLTRERAGFEVRDVHPTH
                     YGRVCPIETPEGPNIGLINSLAIYARVNKFGFLETPYRKVVKGKVTKKISYLSAIDEG
                     QYVIAQANAEIDEKNQLTGDLIAVRYNNEFTLMSADKVQFMDVSPRQIVSIAASLIPF
                     LEHDDANRALMGSNMQRQAVPTILAETPLVGTGMERIVATDSGVTLVAKRGGIIDSVD
                     ASRVVVAVNDVETQSGESGVDIYNLTKYARSNQNTCINQRPLVKAGDLISHGDVIADG
                     ASTSLGELAIGQNMLVAFMPWNGFNYEDSILISEKVVQEDRFTTVHIEELQCIARDTK
                     LGSEEITADIPNIGDAGLRKLDESGIAYIGAEVVAGDILVGKITPKGESQLTPEEKLL
                     RAIFGEKASDVKDTSLRVPSGMDGTVIDVRVFSREGVEKDDRSNAIEDAELDAVRKDL
                     NDQQRIVEDDIYNRIEKILLNKTVVKVGRKKSNNKITKDFLAQISREQWFTIKLKDKH
                     QNSELESYYQRLKLLDNNFQDKLEIARKKIEAPDDLSPGVLKMVKVFLAVKRRIQPGD
                     KIAGRHGNKGVISRVVPVEDMPYMEDGTPIDILLSPLGVPSRMNVGQVLETHLGWAAK
                     ELGLKLDKLLKENKKNKVATIKSYLNKVYNTTAPGRKVSLSQLSNQEILELAENLKNG
                     IPMATPVFDGAHETEIKNLLELSELPSSGQTTLIDGRTGVEYDRPVTIGYMYMLKLNH
                     LVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLRELLTYKS
                     DDVQGRTRMYKQIIDGSNEIETNVPESFNVLMKEIRSLGLNIDLVEDSKASAD"
     gene            complement(11031..11426)
                     /gene="rplL"
                     /locus_tag="QGH35_05640"
     CDS             complement(11031..11426)
                     /gene="rplL"
                     /locus_tag="QGH35_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HJL82048.1"
                     /translation="MTKSTVTSKDEILEAISQMSVMDLVDLISEMEEKFGVSASAPIA
                     VAAVSADASESDEQGDEQTEFDLILQNMGDNKIAVIKAVRAITALGLKDAKDMVESAP
                     VTVKEAVSKGEAEEMKKQLEESGAAVELK"
     gene            complement(11483..12013)
                     /gene="rplJ"
                     /locus_tag="QGH35_05645"
     CDS             complement(11483..12013)
                     /gene="rplJ"
                     /locus_tag="QGH35_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HJL82049.1"
                     /translation="MPLRIEQKKAVVEELNSMASSSVSAAIAEYKGLNVAEITELRTK
                     ARESGVYLKVVKNSLSKQALTGTDFECLTQALQGPIMIALSADDLASPARLFKDFGKD
                     YEHLKTVGLAIDGDAYPSSDLDRIARLPTRDEAYSIIAGLMKAPIEKALRTLNEVPIK
                     LARLILAIKENQEEVS"
     gene            complement(12032..12721)
                     /gene="rplA"
                     /locus_tag="QGH35_05650"
     CDS             complement(12032..12721)
                     /gene="rplA"
                     /locus_tag="QGH35_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HJL82050.1"
                     /translation="MNRLSKRTTANQESVSSEQHYSIDQAIALLKKTGNAKFQETIDI
                     AINLGIDSKKSDQNVRGSTVLPNGTGKLLRVIVFAQGEDADTARNAGADEVGFEDLAE
                     KIKTGFCEFDMVIATPDSMRVVGPLGQILGPKGMMPNPKDGTVTKNLEDAVNHAKAGQ
                     VRFRSDKGGVVHCSIGKIEFSEQSIKENLKSLLDAIIKNKPSSAKGTFIKKVTVSSTM
                     GPGIIIDQSTI"
     gene            complement(12724..13161)
                     /gene="rplK"
                     /locus_tag="QGH35_05655"
     CDS             complement(12724..13161)
                     /gene="rplK"
                     /locus_tag="QGH35_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008285482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HJL82051.1"
                     /translation="MARKKKKIDNVVKLQIPAGQANPSPPVGPALGQAGLNIMEFCNA
                     FNAKTADKEPGVLIPVVISVYGDKSYSFITKTPLASTLLRKAAGVPKGSGEPNINKVG
                     KVTKKQLEKIAEIKNPDLNAIDLEGAVKTIAGTARSMGLEVEG"
     gene            complement(13203..13736)
                     /gene="nusG"
                     /locus_tag="QGH35_05660"
     CDS             complement(13203..13736)
                     /gene="nusG"
                     /locus_tag="QGH35_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008292633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HJL82052.1"
                     /translation="MEYQWYVLQVFSNYEKKVQKMILERSEHIGLADQFADVTVPTEE
                     IVEMKRGKKKTTERKYFPGYLLINMNMNEETWHFVKSIPNVMSFIGGTSEKPSPIPEI
                     EALAILNKAEHSADAPRPKTQYLPGEVVRVIDGPFNDFNGVVEKVNYQKDKLSVAVQI
                     LGRPTPVELNFSQVEKS"
     gene            complement(13762..14133)
                     /gene="secE"
                     /locus_tag="QGH35_05665"
     CDS             complement(13762..14133)
                     /gene="secE"
                     /locus_tag="QGH35_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HJL82053.1"
                     /translation="MNENVKQQQSATLDTIKLIISVLVLLAGIVAYYYFVQAAIFLRV
                     LMILASIPVALTIFYQTTQGKRLWNFFQGSRVEIRKVIWPTRPETIQTTLTVFVFILV
                     LGIFFWLLDFLLLFITTSVTS"
     gene            complement(14159..14234)
                     /locus_tag="QGH35_05670"
     tRNA            complement(14159..14234)
                     /locus_tag="QGH35_05670"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14199..14201),aa:Trp,seq:cca)
     gene            complement(14245..>15323)
                     /gene="tuf"
                     /locus_tag="QGH35_05675"
     CDS             complement(14245..>15323)
                     /gene="tuf"
                     /locus_tag="QGH35_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HJL82054.1"
                     /translation="KFGGDVSAFDDIDNAPEERERGITIATAHVEYESDARHYAHVDC
                     PGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARNVGVPNIVVFLNKADQ
                     VDDAELVELVEMELRELLSEYDFPGDDIPIITGSALKALEGDTGDVGVVAVEKLVATM
                     DEYFPEPERAIDGDFLMPIEDVFSISGRGTVVTGRVERGIVNVNDGVEIVGIKDTQKT
                     ICTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLAKPGSITPHTKFEADIYAL
                     TKEEGGRHKPFFNGYRPQFYFRTTDVTGAVTLVEGTEMVMPGDDAHIIVELIAPIAME
                     EQVRFSIREGGRTVGSGVVTKIIE"
BASE COUNT         4215 a         3317 c         2371 g         5420 t
ORIGIN      
        1 tttgggatgg ccccgccttt tattgcattt acaaattcat aacctgttcc aggctcctgg
       61 ggctctattc taagatagac atgcccatat tgtcctcgac cacccgtctg acgaacaaat
      121 tttgcttcct gttcaaccaa tttttttatt gtctctctat atgcaacctg tggttttccc
      181 acatttgctt ccacgttgaa ttctctagaa agtctatcaa caataatatc aagatgtaat
      241 tctcccattc cagaaataat tgtctgccct gattcttcat ctgttttaac ttgaaatgaa
      301 ggatcctctt tagataattt ctgaagacca atgcccattt tagattcatc ttctgtggat
      361 tttggttcaa cagcaactga gatcacaggc tctggaaact ccatcttctc taaagtaata
      421 atatttttca aatcggctaa tgtatctcct gttgtaacat tttttaatcc aacggcagca
      481 gctatgtctc ccgctctaac ttccttaatt tcttctctag aattggagtg catttgaaga
      541 attcttccta ctctttcttt ttgatgtctc aatgagttat atatggtatc tccagaatta
      601 agaactcctg aataaactct aaaaaatgtc agattcccaa cataaggatc attagctatt
      661 ttaaaagcta gtgctgaaaa gttctcgtca tcatcagcct ttcttaatcc ctctgtgcca
      721 tcttctaaaa ttccataaat tggtggttta tcgattggag agggtaaaaa atgaataata
      781 gcatctagca ttgcttgcac acctttattt ttaaaagcgg aaccacaaat tactggaaca
      841 atttccccat ttaatgttct tgctctcaga ccgctgatga tttgtttaga atccaaagtt
      901 cctgtttcta aatagtgttc aagtaatttc tccgaagttt ctgcagctga ctctagtatt
      961 ttctcatgcc attgcttgca tgcttccatc atgttttccg gaatttcttc ctcagaataa
     1021 gtcactccaa gatcttcatc attccaaata atcgctttca ttctaattaa atcaacaaca
     1081 ccagaaaaac tatcttctga accaattggt agctgaatag gtatcggatg agctcttaat
     1141 ctttgttcaa tttgctcaac aactctcaag aaatcagcac ctgctctatc catcttatta
     1201 ataaatgcta gtcttggaac ggaatattta ttcgcttgcc tccaaactgt ttctgactgt
     1261 ggctccactc ctcctactgc acaaaaaact gcaactgcac tatccaagac tcttagtgac
     1321 ctctcaactt caattgtaaa gtcgacatga ccaggagtat ctatgatgtt gattctatgc
     1381 tgatcaaatt gtttatccat gccttgccaa aaacaagtag ttgcggctga tgtgatggta
     1441 attccccttt cttgttcttg ctccatccag tccataacag cattcccgtc atgcacttcg
     1501 cctattttat gagaaatacc tgtatagaat aatacccttt ctgtagtggt tgttttacca
     1561 gcatcaatat gtgccataat gccaatattt ctatatctat caatgggggt atttcttgcc
     1621 acagtagttg tccagtgctt ttgatgacta ccaacggtag tgagaaaatg ctttatttgc
     1681 ttctgccatt ctatgggttt cttctttctt tttaatcgca tttccacgat ttttggaagc
     1741 gtctaatagc tcattagcta gttttgattt catgttattt tcatttctag aacgagcagc
     1801 ttccattagc caccgcatag ctaaagcctc tcttctatct tgtctaactt caactggtat
     1861 ttgataagtc gcaccaccaa ctcgtcttga tcggacttca acgacaggtt taatattatc
     1921 caatacttca gcaaataaat ctggagctcg ttcgggtggt tctcctaatt tctcgattag
     1981 tgtttctaac gcatcataca caattctttc tgcaacagat ttctttccgt ctatcatcac
     2041 aatattgata aattttgcta acagactgct ggaatattta ggatctggta gaatttccct
     2101 tttggagctt cttgttcttc tagacatgat gaattaattc tttggttttt ttgtgccgta
     2161 cttagacctg ccttgtttcc tgtcacccac tcctgagcaa tccaaagtac ctctgatggt
     2221 atgatatcga acgccaggta agtctttaac tctcccacct ctgattaaaa caatagagtg
     2281 ttcttgaagg ttatgccctt caccaccgat ataacttgta acttcatagc cgttagttaa
     2341 tctgactctg gcaacttttc gtaatgcaga atttggtttt ttcggagtcg tggtataaac
     2401 acgcgtacat acgcctcgtt tttgaggttt gccttctaaa gcaggcacat tattcttata
     2461 ggtcttactc ttcctaggtt ttcttacaag ttgattgatt gtagtcataa tttcttgata
     2521 tctatataat tcgttttgga ttctattctg gtctataaaa aggtgtcaag tcatttaatc
     2581 caattattgt gcttttgttt gatttagatc ttgatcatcc ttatgatcag taggtgtatc
     2641 caggtctcgc aaacccttag aaaattcttc ttcagccagt gatgctttat caatttctga
     2701 cttgaaacca gttccagcag gaaccaatct acctactaca acgttctctt tgagccctct
     2761 taatttatct ttacttcctt ttacagcagc ttctgtcaga actcttgtcg tttcttggaa
     2821 tgatgcagct gatataaagg atgtagttgc caacgctgcc ttagtaatac ccatgagtat
     2881 aggtttaaaa cttgccggtt ttagatcttc agcgatcatt ttttcattaa tttccaacag
     2941 atcagattta tttatttgtt cattgaccaa caattgagta tctccaggat tatcaacttc
     3001 aactgttctc atcatttgcc tgataatcac ttctatatgc ttatcattaa taggaacacc
     3061 ttgaagtcta taaacgtctt gaacttcttt tactaaatat tctgatagtt cttgtatccc
     3121 ttgaacttca attatgtcat gcaaactcac ttcaccatct gcaattacct cacccttcgc
     3181 tatcgtttcg ccctcaaaaa tatttaatct tctagattta aggattagtt cttcatgcga
     3241 attgccatct tcatcggtaa taactagtct aacttttcct ttagtttctt taccaaaact
     3301 ggttataccg gtatattttg cgagtattgc agcacctttt ggctttctag cttcaaataa
     3361 atcagcgact ctcggcaatc cacctgttat gtcgcttgtt tttgatgttt ctaaaggtat
     3421 tcttgcaata acatctcctg aatgaatttt tgcaccatct ttaactccca taattgcacc
     3481 gggtgggaat gcgtatgtca ttggtgtttc tgttccactg aacaatactg gttttccatt
     3541 tgctttttct aatttagctc ctggtctcaa gattttactt gttccaaatt gtttttttga
     3601 atccataatt atgatgctgt caattcctgt catttcgtca atttgttctg tgacggttac
     3661 accatcgata aaattttcaa atttaattac accagctgct tccgcaataa tagggtgagt
     3721 atgaggatcc caagtagaga ttacttgtcc actttttact tttctacctt ctcttacagt
     3781 gataaccgca ccataaggaa tcttatgacg ttctttttct ttaccatgat tatcagttat
     3841 ggatatttca ccagatcgac taatggcaac caaattctta tccgtattac tgattgtctt
     3901 tagattgtgg agatggactt ttccatcagt ttttacatct atactatttg ctgcagcaga
     3961 acttgaagct gctccaccaa tatgaaatgt tctcatagtt agttgcgtcc caggttcgcc
     4021 gattgattgt gcagctatga ttcccacagc ttcccctttg ttgactatat ttcctctcgc
     4081 aagatctctt ccataacatt gagaacaaac accatgtttt gtttcacatg taactgggga
     4141 tctaacctgg attctttcta tgccatgtag ttcaaataac tctacatttt tttcatcaat
     4201 tagctctcca accttaagaa tttttttatt atctttatct aataacggtt cagcaataat
     4261 cctgccaagg attctatcac caagtttttg aacaatttca ccaccttcaa taattgcaga
     4321 tattattatt ccctgacttg tttcacaatc ttcctctgat acaaccaaat cttgtgagac
     4381 atcaactaat cttcttgtca agtacccaga attagccgtt tttagtgccg tatcagcaag
     4441 accctttcga gcaccatgtg ttgatataaa atactgaaaa acatttaaac cttctctgaa
     4501 gtttgctgtt attggtgtct caataattga accgtcgggc tttgccatta atcctctcat
     4561 tcctgccaac tgtcttatct gtgctgcaga acccctagct ccagaatctg ccatcatgaa
     4621 tattgagtta aaagaaggtt gcgtaaactt ttcgccagcc tgattttcaa cttcttctga
     4681 tccaagtgta tccatcatag cttttgaaat aagctcattt gttcttgacc aaatatcaac
     4741 cactttattg tatctttctc cttgagtaag aagccctgaa gagaattggt tttgaatatt
     4801 tctaacttgt tcttctgctg cactgagaat tttatctttt aattctggaa ctaccatatc
     4861 atcgagtgaa attgaaattg ctgcttgtgt agcatgctga aagcccatat acattagctt
     4921 atctgcaaat acgactgccg cttttaaccc aacgtctctg taacatttat tgattaaact
     4981 agaaatctgc ttactatcca ttgttttatt tatcaatgag aacggaagtt cttttggcac
     5041 tattgttgaa agtattgctc ttccaaccgt tgtttgaact aattcatagt ttgcttctac
     5101 ttcttcttta gaatcattat ttgtgggtat cctaacttga acttttgcat gtatgcctac
     5161 atgaccactt tcgtgtgctc taataagctc cgttatgtct gcatagattt gtccttctcc
     5221 caaagcatta actttttctc tggtcatata atacaaaccc aaaacaatat cctgagatgg
     5281 aacgattatt ggttcaccat ttgctggaga caaaatatta ttagaagaca tcattaaagc
     5341 tctagattca acttgagcct caattgataa tgggacatga actgccattt gatctccatc
     5401 aaaatctgcg ttaaaagctt tacaaaccaa tggatgtaat tggatagctt ttccttctat
     5461 aagaactggt tcaaaagctt gaatacctaa cctatggaga gtgggggcac gatttaacaa
     5521 gactgggtgt tctcttataa cgttgtcgag aatatcccaa atttctggcc cctgaagctc
     5581 tacaagcttc ttcgctgctt tgattgttga tgcttctcct tggtagataa gcttactaaa
     5641 aacaaacggt ttaaagagtt caagtgccat cttttttgga ataccgcatt catgcagttt
     5701 caacgtaggc cctacaacaa tcacagatcg tccagaatag tcaactcgct tacctaagag
     5761 gttttgtctg aacctgcctt gtttgccttt aatcatatca gccaatgatt ttaatggtcg
     5821 cttatttgat ccagttatag ctctgcccat tcttccatta tcaagaagag catctactga
     5881 tttttgtaac attctttttt cattacgaac aataatttca ggagcattta attccaataa
     5941 tcttttaagt ctgttatttc tattgattac ccttctataa agatcattaa gatcagatgt
     6001 tgcaaatcta cctccatcta acggaacaag tggcctcaga tcaggcggaa gaattggcaa
     6061 atttttcaaa atcatccatt caggtctgtt tcctgatttt attaatgatt caactaattt
     6121 taatctttta gaaaggcgct taagtttaat ttgtgaagca gtagcattca tgtcttcatg
     6181 aattttttgg agttggtctt gtaatttaat atcttttaag actccatgaa ttgcttctgc
     6241 tcccatttga gcctcaaaat cgttgccaaa ttccccaagt gcttcgatat actcttcttc
     6301 agaaagtaat tggcccattt ctagcgttgt cattcctggc tcaacaacta aatatgcttc
     6361 aaaatataaa accctttcaa tttcacgcaa tgtcatatct aaaagtaaac caacccgaga
     6421 gggaagtgat tttaaaaacc aaatgtgggc tactgggctg gctaattcta gatgccccat
     6481 gcgttctctt ctaaccttac tttgagtaac ctctacgcca cacttatcac aaacaacacc
     6541 tctgtgtttt agccttttgt atttaccaca taaacactca taatctttga caggtccaaa
     6601 aatttttgca caaaataaac catctctttc tggtctaaat gttcggtaat taatagtttc
     6661 tggttttttc acttcaccgt atgaccacga tctgatcatt tctggggaag ctaaacctat
     6721 ttttatataa tcaaactctt ctgtctgatt tttctgtttg aaaaaattta ataggttttt
     6781 catagtgata gctctctgtt attagtcagc agaagccttg gaatcttcta ctaaatcaat
     6841 attcagtcct aatgacctaa tttctttcat taggacatta aacgactcag gcacatttgt
     6901 ctcaatctca ttactaccat caataatttg cttatacatt cttgttcttc cttgaacatc
     6961 gtctgattta taagtaagta actcacgtaa tgtatgtgca gctccataag cttcaagtgc
     7021 ccatacttcc atttcaccaa atctctgccc tccaaactga gcttttcctc caagcggttg
     7081 ctgtgttacg aggctataag gacctgttga tcttgcgtgc atcttgtcat ccacgagatg
     7141 gtttaatttg agcatataca tataccctat ggttactggt ctgtcatatt caacgcctgt
     7201 tctgccatct atcaatgtag tttgccccga agaaggaagc tctgataatt caagtaaatt
     7261 ttttatttca gtttcatgag ctccatcgaa aacaggagta gccatgggta taccattttt
     7321 taaattttct gctaattcta aaatttcttg attcgataat tgagacaagc tgactttcct
     7381 gcctggtgct gttgtattgt atactttatt cagatagctc ttgattgttg caactttgtt
     7441 tttcttattc tcctttaaaa gtttatcaag ctttaagcct aattcttttg cagcccatcc
     7501 taaatgagtc tctaagactt gaccgacatt cattctagaa ggaactccta aaggacttaa
     7561 cagaatatca attggtgttc catcctccat ataaggcata tcttccacag gaactactct
     7621 tgagatgaca cctttattac catgtcttcc agcaattttg tcacctggtt gtattcttct
     7681 ttttacagct agaaaaacct taaccatttt taagacgcct ggcgacaaat catctggggc
     7741 ttctatcttt ttccttgcta tttctaattt gtcttgaaaa ttgttgtcaa gaagctttag
     7801 tcgttgatag taactctcca gttccgaatt ctgatgttta tcttttaatt taattgtgaa
     7861 ccattgctct cgacttatct gtgctagaaa atctttagtt attttattat tcgatttttt
     7921 cctaccaacc ttaactacag ttttatttaa aagaattttt tcgatacggt tgtaaatatc
     7981 gtcttctaca attctctgtt gatcatttaa gtcttttcta actgcatcta actcagcgtc
     8041 ttcaattgca ttagatctat catctttttc aacaccttct cttgaaaaaa cccttacatc
     8101 aataactgtc ccatccatac cagaaggaac cctaagtgaa gtatctttta catcgctagc
     8161 tttctctcca aaaatggctc ttagtagttt ttcttctggt gtcagttgtg attcaccttt
     8221 tggtgtaatt ttgccaacta gaatatctcc ggcaactact tctgcaccga tgtaagctat
     8281 tcctgactca tctagttttc ttaaaccagc atctccaata ttaggaatat cagctgtgat
     8341 ttcttctgat cctaatttcg tatctcttgc aatgcattgc aattcttcaa tatggactgt
     8401 tgtaaaccta tcctcttgaa ccactttttc agaaataaga attgagtctt cataattaaa
     8461 tccattccac ggcataaatg ccaccagcat attttgacca atcgctaatt cacctaaact
     8521 ggtagaagcg ccatcagcaa taacatcacc atggctgatt aagtctcctg ctttgaccaa
     8581 tggcctctgg ttaatacatg tattttgatt ggatcttgca tattttgtta gattataaat
     8641 atcaactcct gattctccag actgagtttc aacatcattc acagcaacaa ccaccctaga
     8701 cgcatcaaca gaatcaatga tgcctcctcg ttttgcaacc aaagtaactc cggaatcggt
     8761 agcaacaatt ctttccatcc cagttccaac aagaggcgtt tcagctaata ttgttggcac
     8821 cgcttgtctt tgcatattgg aacccattaa tgcacggtta gcatcatcat gttcaaggaa
     8881 tggtataaga gaagctgcga ttgaaacaat ttgccttggt gacacatcca taaactgaac
     8941 tttatcagca gacatcaaag taaattcatt attgtatcta acagcaatga gatctcctgt
     9001 cagttgattc ttttcatcaa tctcagcatt tgcttgggcg ataacatatt gcccttcgtc
     9061 aattgcagat agataactaa tttttttagt taccttacct ttaactactt ttctataagg
     9121 tgtttctaaa aatccaaatt tgttaactct tgcataaatt gccaatgaat tgattaaacc
     9181 aatattaggg ccttcaggtg tttcgatggg acacactcta ccataatgag taggatgaac
     9241 atctctcacc tcaaagcctg ctctctctcg agttaatcct ccagggccta aagctgaaat
     9301 tcgtcttttg tgtgagactt cagaaagtgg gttattctga tccataaatt gagacaactg
     9361 acttgatccg aagaattctt ttattgcagc tgaaactggc ttagaattga tcaaatctct
     9421 tggcatcagt tcttctgtct caactaacga aagtcggtct ttcacagctc tttcaaccct
     9481 aactaagccg actcgaaatg cattttcagt catttcccca actgttctta tccttctatt
     9541 tccaaggtgg tcaatgtcat caacagtatc tttaccattt cttatattga tcaatgtttt
     9601 caatacatca ataatgtctt cgttattcaa agaattattg ccaagatctg attttctgcc
     9661 tattcttcga ttgaacttca ttcttccaac ttcagataga tcatatcgat cctcattaaa
     9721 aaagagattc tgaaatagat tttctgatgc ctccttagtc ggaggttcac ctggtctcat
     9781 catcctgtaa atttccgtta atgcttcctg ttttgtggaa gatggatcaa ttctcattga
     9841 atttgatatg tagtcaccat gatctaaatc gttaatatgg attgtttcta ttgcttttac
     9901 tccattttca ataaggatat ttattttctc gatagataat atttcattag taagcgctag
     9961 taactcaccc gttgtgggct cgactacatc atgagcaatc accttgcctt caagatgatc
    10021 aacaggaaca gttagaaatt tatttttttt gctcaactcc tctaaccgtc ttaaatgccc
    10081 tccattgatc cgctgccctt tcttgataat gacttttctt ccaattttaa tatcaaaatc
    10141 tgcagtaata cctttgaagt gatctggctt gaagtgtagt tttatttctt ttttagaaag
    10201 actgaatttg tccttttcaa agaaaatatc taggatttct tcattcgaca agcctagagc
    10261 atgaagcatg atcgttacag atatttttct tcttcggtca attcttgcaa aaattgcatc
    10321 tttcgcatcg aattcaaaat ctaaccacga acccctataa ggaataatgc gagcagagaa
    10381 tagaagtttt cctgagcttt gaactccttt atcatgatca aaaaatacac ctggtgatct
    10441 atgtaactgt gaaacaatga ccctatccgt tccattaata acgaatgttc catagtcagt
    10501 cattaaagga atctcaccaa aataaacatc agatgtatct cctgattctt tgatgttttt
    10561 tacttccttg accgtttctt cacccgtttc cttgttatat gtaattagtt ttgttttcac
    10621 tctgatcggt gaagcatagg ataaacctct aagttgacat tctttaacgc taaatccagg
    10681 atcatccaat ctataactaa caaattctaa tcgtgcattc tttgaaaaac tctcaattgg
    10741 gaagagtgta ttaaaggctg cttctaaacc taaatttttt ctttttgctg ggtcgacatt
    10801 tcgttgtaaa aatttattgt aggatgctaa ctgtattgac agtaaatcag gtacatctag
    10861 tactgattgt tgttttccaa aatcttttct aattcgtttt ttttcagtaa atgaatatgc
    10921 catacgtctg attgttttaa taatgttcaa gcaacagcaa acctgcccgt ttgctgagcc
    10981 tcaattgttt gttatacaaa atacatgcag aataaatgag ctagtacgat ctacttaagc
    11041 tctacagcag ctcctgactc ttctaactgc ttcttcatct cttctgcttc accctttgat
    11101 actgcctctt ttacggtaac aggcgcactt tcaaccatat cctttgcatc tttaagccca
    11161 agtgctgtaa ttgctcttac agccttgata acagcaattt tattatcccc catgttttga
    11221 aggatgagat caaattctgt ttgttcatct ccttgctcat cagattctga agcgtctgca
    11281 gaaactgccg ctacagcaat cggcgctgat gcactgacgc caaatttttc ttccatttct
    11341 gaaatcaaat ctacaaggtc catcacagac atttgtgaaa ttgcttctag aatttcatct
    11401 tttgatgtta cggtactttt agtcattatt ttctcctcga atcaacccaa aaaagacggg
    11461 cattttatat aaaaaaaact tattaggaaa cctcttcttg attttcttta atagcaagta
    11521 tcaatcttgc tagcttaatc ggtacttcat ttagtgttct aagtgctttc tcgatgggtg
    11581 ctttcatgag accagctatt atagaatagg cttcatctct tgtaggtaat cttgcaattc
    11641 tatccagatc tgaagaaggg tatgcatcac catctatagc taaaccaact gttttcagat
    11701 gttcataatc tttaccaaaa tctttgaata accttgcagg tgaagctaaa tcatctgcag
    11761 agagggcaat cattattggt ccttgcaatg cctgagtcaa acattcaaaa tctgtacctg
    11821 tcagtgcttg tttgcttaag ctatttttga ccactttaag ataaacgccc gattctcttg
    11881 cttttgtgcg caattctgta atttcagcaa cattaaggcc tttgtattct gcaatggcag
    11941 ctgacactga tgaagaagcc attgaattta gctcttcaac gactgctttt ttttgctcta
    12001 ttcttaaagg cataattaat tcctaatact attagatagt gctttggtcg atgataattc
    12061 caggacccat tgttgatgaa acagttactt ttttgatgaa tgtgcctttg gctgaggatg
    12121 gtttattttt tataattgca tctaataaag attttaaatt ctcttttata gattgttcag
    12181 aaaattctat tttccctatt gaacaatgaa cgactccacc tttgtcgctt ctaaatctta
    12241 cctgcccagc ttttgcatga ttgaccgcgt cttccagatt ctttgttact gttccatctt
    12301 tagggttggg catcatccct ttgggtccta gtatttgtcc tagtggtcca actactctca
    12361 ttgaatctgg tgttgctatg accatgtcaa attcacaaaa gcctgtttta attttctcag
    12421 ctaaatcctc aaaaccaacc tcatcagctc ctgcatttct tgctgtatct gcatcttctc
    12481 cttgtgcaaa aacaataact cttaacaatt ttccagtacc atttggcaaa actgtggatc
    12541 ctctcacatt ttgatctgac tttttagaat ctatacctaa attaattgca atatctattg
    12601 tttcttgaaa tttagcattc ccagtttttt tcaaaagagc aatggcttga tcaatactat
    12661 aatgttgttc agagcttact gattcttgat tcgctgtggt tctttttgaa aggcgattca
    12721 tgattaacct tcaacctcca aacccatgct tctggctgtg ccagctatag tctttactgc
    12781 cccctctaga tcaatggcat tgagatcagg gttttttatt tcagctattt tctctaattg
    12841 cttttttgta actttgccaa ctttatttat attcggttcc ccactgccct ttggaactcc
    12901 agcagctttt cttaataacg tcgaagcaag aggagttttt gtaataaaac tataactttt
    12961 atcaccataa acactaatca ctacaggaat taatactcct ggttctttat cagcagtttt
    13021 tgcattaaaa gcgttacaaa attccatgat attgagtcca gcttgtccta aagcagggcc
    13081 aacagggggg cttggattag cttgacctgc tggtatctgt agttttacca cattgtctat
    13141 ttttttcttt tttctagcca tttctctata cgttatatat cagtgatttc ttatggttaa
    13201 tattaagatt tttctacctg actaaagttc aattcaacag gagtaggtct tcccaaaatc
    13261 tgaacagcta cgctcagctt atctttttga taattgactt tctcaacaac accattgaaa
    13321 tcattaaaag gtccatcaat aaccctaacc acttccccag gtaggtactg agttttgggc
    13381 ctgggagcat cggcagaatg ctcagcttta tttaatatag ccagtgcctc aatttcaggt
    13441 attggcgatg gcttttcact agttcctcca ataaagctca tcacgttagg aatggacttc
    13501 acaaaatgcc atgtttcttc attcatattc atattaatga gtagataacc agggaaatat
    13561 tttctttcag tagttttctt cttccctctt ttcatttcaa caatctcttc tgttggaaca
    13621 gtcacatctg caaattggtc agcaagccct atgtgctcac ttctttccag aatcattttt
    13681 tgtacttttt tctcataatt tgagaagacc tgtaaaacgt accactgata ttccattctg
    13741 atgcctcttt taattaatct gttaagaagt gacagaagtt gtaataaaaa gcagtaaaaa
    13801 gtctaagagc caaaagaaaa tccctaatac taaaataaag acaaacactg tcaaagtcgt
    13861 ctggattgtt tctggtcttg tcggccaaat aacttttcta atttcaacac gtgaaccttg
    13921 aaaaaaattc cacaatcttt ttccctgcgt ggtctggtag aaaatagtta aggcaacagg
    13981 aattgaggct agtatcatca aaactcttag aaaaattgca gcctgaacaa agtaataata
    14041 agcaacaata cctgccagaa gtaccaacac tgaaataatt agcttaatgg tatcaagcgt
    14101 agccgattgt tgttgtttta cattttcatt cattgcatta atccaatggt tctaagtctg
    14161 gcaggccagg agggactcga acccccaacc tgcggttttg gagaccgcca ctctaccagt
    14221 tgagctactg gcctttaatt agttttactc aattatcttt gtaaccacac cagacccaac
    14281 agttcttcca ccttcacgaa tagagaagcg aacctgttct tccatggcta ttggtgcgat
    14341 caactcaaca attatatgag catcgtcacc tggcataacc atttctgttc cttctacaag
    14401 ggtgacagca cctgttacat ccgttgttcg gaaataaaac tgaggacgat aaccattaaa
    14461 gaaaggtttg tgtcttccgc cttcctcttt cgtaagggcg taaatatcgg cttcaaattt
    14521 tgtatgaggc gtaatactac ctggtttagc aagaacttgt ccacgttcaa catcttctcg
    14581 ttttgttccc cgtagaagca ctccaacatt atctccagct tgtccttgat caagtaattt
    14641 tctaaacatt tctactccag tacaaattgt tttctgagtg tctttaattc caacaatttc
    14701 taccccatca ttgacattga cgattcctct ttccacacgg cctgtaacca cagttcctct
    14761 tcctgatatg gaaaaaacat cttctatagg cattaagaaa tcaccgtcaa tagctctttc
    14821 aggttcagga aagtattcgt ccatagtagc cactaacttt tcaacagcca caacacccac
    14881 atcaccagta tcaccttcta gagctttgag cgcacttcca gtaatgattg gaatgtcatc
    14941 accaggaaaa tcgtattcag agagtaattc cctgagttcc atttctacta gttcaaccaa
    15001 ttctgcatca tctacttggt ctgctttatt aaggaagacg acaatattag gaacacctac
    15061 atttctcgct aacaaaatat gttctctggt ttgtggcatt ggtccatcag ctgcggaaac
    15121 caccaaaatt gcaccgtcca tttgtgcggc accggtgatc atattcttaa cataatcagc
    15181 atgtccagga caatcaacat gagcataatg tctagcatca gactcatatt caacatgcgc
    15241 agtggctata gtaatgcctc tctcacgttc ttccggagca ttgtctatgt catcaaatgc
    15301 agaaacatct ccaccaaatt tat
//
LOCUS       DASCTH010000268         3804 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000584798, whole genome shotgun sequence.
ACCESSION   DASCTH010000268 DASCTH010000000
VERSION     DASCTH010000268.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000584798"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="QGH35_05680"
     CDS             <1..215
                     /locus_tag="QGH35_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009556721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82055.1"
                     /translation="LTPQHPGLLNGMRKNMAYEEREFTIKTTEGKEFTQSRKVPIGSN
                     WPSKKPNQPPKPSIVAVRRLDNKRIT"
     gene            369..2531
                     /locus_tag="QGH35_05685"
     CDS             369..2531
                     /locus_tag="QGH35_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL82056.1"
                     /translation="MKTLKRKNCILIQLVLLISIFIIAPAWAQIEEIIVTAERRSEGI
                     QDVPLSISAFSSDAMEKKQIDVTKDIAFHVPNLLAYTLTAGGDAIQVHMRGVSVQNGG
                     MMTSETPVGFYEDDIYRGRMAAINTQLTDIERVEVLRGPQATLYGRNTISGAVKIISR
                     TPGDEAWKNLSIGYGNYETSLITASLGGPIEEGSLSGSIALAYGDRGKGYFDNTTGGH
                     PGEYDRHAFRGKLHWYENDGVDIVASVFSSGGKNDGLNAVPYGPDFDPPTLPTGEPLA
                     GFYCACSAHQSTGDSDHSGGTLHVTVDLDTGAILKSITAITDLDDHFSFDLNGGGYQV
                     APGVAITGIQGFTLTSNGESDQFSQEFQLSGKAKDDQLDWIVGLYYMEEEGSQSFSGG
                     MGGDPFFTEHTTFKTDSVAFFTQLTYQMSDNLSLTAGARYTEDDKEYTNQCISFFMCV
                     GPLTVVLDESFTETTPKLSLEYQWKENVMVYAVVASGFQAGGFQTLCIANMNCAGNIY
                     DPQTVWNYEIGIKSDLLDNTLRVNASTFVAKYEDIQQTIITGNAYPQGNVGEVDVDGI
                     ELEMTWVASENLSLFATLGLMDSDYGKVAANSPVYATGADTLPSTPDYSFNAGFDYAW
                     NVQHPFYNDLEVFLGANLFKTDDYFSEATNALLIPGYSLVNAYVGIRPLDGRWQVSLR
                     GKNITDEEYYVSGIAGAGTNIRTTEPPAEYMLTAKFNF"
     gene            complement(2594..>3804)
                     /locus_tag="QGH35_05690"
     CDS             complement(2594..>3804)
                     /locus_tag="QGH35_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014794.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJL82057.1"
                     /translation="AVSGNPGPAFLEIPMDVLMGMVEKDDIRFPTMRSKAPLMIPNKT
                     DVQEVVELLKVAKKPVMMAGTSVKWSNASATVQKFIEKTHIPTFVNGMGRGTVKPGTP
                     ELLNRVRKESLQQCDLFICAGVLLDFRLNYGDTIAADAKIIQLDVENELIGTNRSADA
                     SVVGNLNASFDMIIELMEETGDSLDYSSWRDQLIEREGQLEAEFSSQLNSDEVPIDPL
                     RLCREIRDFVAEKDIILIGDGGDIVAQAAKVLPAPAENSWMDPGPLGTLGVGMPFALA
                     AQITRPDKRVLIIYGDGSFGLNGFEYDTALRFNLPIVGIVGVDGAWGQMARPQAAVYG
                     AKRVIATTLNFTRYDKIVEAMGGHGEFCERPEEIGPALERAFASGKAALVNVVMRQDI
                     DTGMKGSTYA"
BASE COUNT         1110 a          772 c          817 g         1105 t
ORIGIN      
        1 ttctcacccc acaacaccct ggattattaa atgggatgag aaaaaatatg gcttatgagg
       61 aaagagaatt tacaatcaaa acaaccgaag gtaaggaatt tacccaatct cgaaaagtcc
      121 caattggctc taattggccc tctaagaaac ccaaccaacc cccaaaacca tcgattgttg
      181 ctgttcgtag actagataat aaaagaatta cttaaggaaa taagaaatct agcaatagat
      241 cgatttatct gtcagatttt tcatatttga tatagatcaa ttacaaaatt atcacatata
      301 cctattatct agtctctatc taagggatat cctctttact atttctgtca acattaatgg
      361 agttttttat gaaaacactt aagagaaaaa attgtatatt aattcaatta gttttactga
      421 tttctatatt tataattgca cctgcttggg cacaaataga ggaaattatc gttacggcag
      481 aacgacgatc agaaggtata caagatgttc ctctctctat tagtgcattc tcgagtgacg
      541 caatggaaaa aaagcaaata gatgttacta aagatatagc gtttcatgtc ccaaatcttc
      601 ttgcctatac tctaactgca ggtggtgatg ctattcaggt acacatgcgt ggcgttagtg
      661 ttcaaaatgg tggcatgatg acatctgaaa ctccagttgg tttctatgag gacgatattt
      721 atagaggaag aatggctgct ataaatacac agttgactga tattgaacgt gtggaggttc
      781 taagaggacc acaggccact ctttatggga gaaatactat ttctggtgca gtaaagatta
      841 tttcaagaac gccaggagat gaggcttgga aaaatttatc aataggctat ggtaattacg
      901 aaacatctct cattaccgcg tctcttggtg gacccattga ggaaggaagt ctctcaggtt
      961 ctatagcttt ggcctatggt gaccgtggta agggttattt tgataacact acgggagggc
     1021 atccaggtga atatgatagg catgccttta gaggcaagct tcattggtat gaaaatgatg
     1081 gggtagatat tgttgccagt gtattcagtt cagggggtaa gaatgatgga cttaacgcag
     1141 tcccttatgg accagatttt gatccaccaa cacttccaac tggagaacct ttggcaggtt
     1201 tctactgtgc ttgttcagca catcaatcca caggggattc ggatcattct ggaggaacac
     1261 ttcacgtcac agttgattta gatactgggg ctatactaaa gtcaattaca gccattacag
     1321 atctggatga tcatttcagc tttgatctga atggaggtgg ctaccaagtt gcacctggtg
     1381 ttgccatcac tggtatacag ggctttacgc ttacaagcaa tggcgaatca gatcaattta
     1441 gtcaggagtt tcagctctct ggtaaagcaa aggatgatca actggattgg atagttggcc
     1501 tgtactacat ggaagaagag ggtagtcagt cctttagtgg cgggatgggc ggtgacccat
     1561 tctttaccga gcacacaacg tttaaaacag atagcgttgc attttttact caattaactt
     1621 atcaaatgtc agacaacttg tctcttaccg cgggagcgcg ttacacagaa gatgataaag
     1681 agtataccaa tcaatgtata agctttttta tgtgtgtcgg tccgctaact gttgtccttg
     1741 atgaaagctt tactgaaaca acacctaagc ttagtttgga gtatcagtgg aaagagaatg
     1801 taatggttta tgccgttgtt gccagtgggt ttcaagcagg ggggtttcaa actctttgta
     1861 tagccaatat gaattgtgca ggcaatatat atgatccaca aaccgtttgg aattatgaaa
     1921 ttggtataaa aagtgacttg cttgacaata cactgcgagt caatgcctct acctttgtag
     1981 caaagtatga agacatacaa caaactatta ttacaggaaa tgcatatcct caaggcaacg
     2041 ttggtgaagt tgatgtggac ggtattgagt tggagatgac ttgggtagca tcggaaaatt
     2101 taagtctttt cgcaacgttg ggattgatgg atagcgatta tggaaaggta gcagccaatt
     2161 ccccagttta tgcgacaggt gctgacactc ttccttcgac tcctgattac tcattcaatg
     2221 ctggttttga ttatgcgtgg aacgtccaac accccttcta taatgatctt gaagtttttc
     2281 taggagctaa tctgtttaaa accgatgatt atttttcaga agcaacaaat gctttactaa
     2341 tcccaggcta tagcttggtt aacgcttatg ttggtataag gcctcttgat gggcgttggc
     2401 aagtatcact gaggggtaaa aatataactg atgaagaata ttatgtctcg ggtattgctg
     2461 gtgctggaac aaacatcagg accacagaac cacccgctga gtatatgtta acagcaaaat
     2521 ttaacttcta aaaactgata tagaaaagca aaatctgttc ggaattgaac ttagctcaca
     2581 gttttgatta tctctaagcg taggtgctgc ccttcatgcc cgtgtcgata tcctggcgca
     2641 tgactacatt gactagtgcc gctttgccag atgcaaaagc tcgctccaag gctggaccga
     2701 tctcttcggg tcgttcacag aattcaccat gaccgcccat tgcttcgaca attttatcat
     2761 aacgggtaaa gttgagcgtc gtggcgatga ctcgtttagc accatagaca gctgcttgtg
     2821 gcctagccat ttgtccccaa gcaccatcaa cgccaacgat gcctacaata ggcagattaa
     2881 atcgcagtgc tgtatcatat tcaaagccat tgagaccaaa tgagccatca ccgtaaatga
     2941 tcaatactct tttgtctggt ctggtaatct gtgctgctag agcaaatggc atgcctaccc
     3001 ccaaggttcc tagaggaccc ggatccatcc agctgttttc agctggtgct ggtagtactt
     3061 tagcagcttg ggcaacgata tctcccccgt caccaatcag aatgatgtct ttttctgcca
     3121 caaaatcacg aatttcacga cacaaccgca ggggatcaat cggtacctca tcagaattga
     3181 gttgtgaaga gaattcagct tctaattgtc cttctcgttc gatcaattga tctcgccagc
     3241 tagaataatc taaactgtca ccagtttctt ccataagctc gattatcata tcgaaactgg
     3301 catttagatt gccgacaaca gaagcatcgg ctgatcggtt agttccaata agttcattct
     3361 caacatcgag ttgaataatt ttggcatctg ccgctatggt atctccataa ttaagacgaa
     3421 agtccaacaa cacaccagca caaataaaca agtcacattg ttgcagagat tccttgcgta
     3481 cacgattcaa gagttcggga gtgcctggct ttacagttcc tcgtcccata ccgtttacaa
     3541 aggtaggaat gtgggttttt tcaataaatt tctgaacggt agcagatgcg ttggaccact
     3601 tgacgctggt accagccatc atcacaggct tcttggcaac tttgagtaat tctaccactt
     3661 cctgtacatc cgttttattc ggtatcatga gcggtgcttt tgaacgcatg gtcggaaagc
     3721 gaatatcatc tttttcaacc atgcccatca gtacgtccat cgggatctct aagaaggctg
     3781 gacctggatt acctgatact gcat
//
LOCUS       DASCTH010000269         1606 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000586323, whole genome shotgun sequence.
ACCESSION   DASCTH010000269 DASCTH010000000
VERSION     DASCTH010000269.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000586323"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="QGH35_05695"
     CDS             complement(<1..418)
                     /locus_tag="QGH35_05695"
                     /inference="COORDINATES: protein motif:HMM:NF027503.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HJL82058.1"
                     /translation="MSEEKIESILNETRRFNPPKDFVSRAQLTKEKLTTLHAQALKNY
                     EEFWSDLGREEIHWFEPFNKTLDESQAPNYKWFIDGKLNASYNCLDVHIDSNAEKTAI
                     IFEGENGEIRRLSYADLLKEVCLFANGLLSIGVKPLE"
     gene            complement(432..1304)
                     /locus_tag="QGH35_05700"
     CDS             complement(432..1304)
                     /locus_tag="QGH35_05700"
                     /inference="COORDINATES: protein motif:HMM:NF015818.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HJL82059.1"
                     /translation="MSIPIKEIAGVSGLSLMEPEYITTPTPGQVIKLKVRSVDNNYFL
                     KLGLLTEYPLLVAEQTGLDALKSAQAVKIPEVVFIGTTSTHSLLITEYLELKANNFLS
                     GKRLGEQLAALHRHTNEFFGLGEDNWIGLNPQMNNWSANWTDFFIKYRLSPQCLMASR
                     KGSQSIIEKKVNLLMESMDLFFTQYKPQPSLLHGDLWSGNWGTINEETPVIFDPAVYY
                     GDREADLAMSKLFGGFPNIFYEAYNHSWPLDSGFNLRQNLYNLYHLLNHLNLFGEAYL
                     NQTNAVLDQLLSEI"
     gene            complement(1320..>1606)
                     /locus_tag="QGH35_05705"
     CDS             complement(1320..>1606)
                     /locus_tag="QGH35_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HJL82060.1"
                     /translation="RKDLTRDHIKPLSRGGLDTWQNVVTACKRCNHHKGGKTPEEAGI
                     ELLAIPFVPNYAEYIYLKGRNILADQMDFLMGYISDASPIHRRMHLQKKA"
BASE COUNT          468 a          311 c          285 g          542 t
ORIGIN      
        1 gttctaaggg cttgacacct atgctcaaca atccattagc aaataaacaa acctctttta
       61 aaagatcggc atagcttaat ctcctaatct ctccattttc tccttcaaaa attattgctg
      121 ttttctcagc gtttgaatca atgtgaacat ctagacagtt atatgaggca tttagtttgc
      181 catctataaa ccatttataa ttaggcgctt ggctttcatc tagagtttta ttaaatggtt
      241 caaaccaatg gatttcttct cggccaaggt cagaccagaa ttcctcatag ttttttaaag
      301 cttgtgcatg cagcgttgta agcttttctt ttgttaattg tgctcgtgaa acaaagtctt
      361 taggggggtt aaatcgtctt gtttcattta gaatggattc tattttttct tcgctcattg
      421 agtaatctcg cttatatttc tgaaagcaat tggtctaaga cagcatttgt ttggtttaaa
      481 tacgcttcac caaaaaggtt caaatgattt aataaatgat acaggttata tagattttgc
      541 ctcagattga atcctgaatc tagtggccat gagtgattat aagcttcata aaaaatattg
      601 ggaaagcctc caaataattt agacatagca agatcggcct ctctgtcgcc atagtagaca
      661 gctgggtcaa aaatcacagg agtctcttca ttgattgttc cccaatttcc agaccataaa
      721 tcgccatgca gcaacgaagg ttgtggcttg tattgagtaa aaaaaagatc catagactcc
      781 atcaataaat ttactttttt ttctattatt gattgagatc ctttccttga tgccattaaa
      841 cattgtggcg aaagcctgta ttttataaag aaatctgtcc agtttgctga ccaattgttc
      901 atttgagggt ttaaaccaat ccaattatct tcacctaggc caaaaaactc attcgtatgt
      961 cgatgaaggg cagctaattg ctcacctaac cgtttgccag ataaaaaatt attggcttta
     1021 agctcgagat attcggtaat aagcagggag tgtgttgaag ttgtgcctat aaaaacaacc
     1081 tctggaattt taaccgcttg tgctgacttt aaagcatcta ggccagtttg ctctgcaacc
     1141 aataatggat actctgtcaa caagcctagt tttaaaaaat aattgttatc gacgcttcta
     1201 actttaagct ttataacttg tcctggagtt ggtgtggtta tatattcagg ttccatcaaa
     1261 gacagtcctg aaacacctgc tatttctttt attggaatcg acaagaataa atttaagtat
     1321 tatgcttttt tttgaaggtg catccgccta tgaatgggac ttgcatcgga gatatacccc
     1381 ataagaaagt ccatttggtc agcaagtata tttcttcctt tcagataaat atattcggca
     1441 tagtttggca caaaaggaat ggctaaaagc tcaattcctg cctcttctgg agtcttcccg
     1501 cctttgtgat gattgcaacg cttacaagct gtcacaacat tttgccacgt atctaagccc
     1561 cctcgactca agggcttgat atgatctctt gtaagatcct ttctta
//
LOCUS       DASCTH010000270         8396 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000590519, whole genome shotgun sequence.
ACCESSION   DASCTH010000270 DASCTH010000000
VERSION     DASCTH010000270.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000590519"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="QGH35_05710"
     CDS             <1..627
                     /locus_tag="QGH35_05710"
                     /inference="COORDINATES: protein motif:HMM:NF015691.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HJL82061.1"
                     /translation="MKKIIVACQKNKISIGAHPSYPDQISFGRRTIEITESKLKESLT
                     HQINRLLDLSKELNTKLTHVKAHGALYHDVSTSNNVAALFVDVINQIDPNLYILGPPG
                     SAIESVVEEHRMHFICEAFLDRRYSDSGFLLNRSLQGAILENISERCEQAVNIVCHHK
                     VQTETGGFFNLKAETLCLHSDSPNAVKTAARVCSELAKVNITIQSFVE"
     gene            624..1328
                     /gene="pxpB"
                     /locus_tag="QGH35_05715"
     CDS             624..1328
                     /gene="pxpB"
                     /locus_tag="QGH35_05715"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HJL82062.1"
                     /translation="MKKIVNPPIIHYGDDALLVKIQHLALTCKPNEYALALLAYLRAS
                     NNDWIDVVPAEESLAVRYSPFRLSAKEAYSNLEKQIDEFDYQPLTQNNQLIHIPVCYH
                     DKFAPDMHTVTQQKVLSREEVIKIHSSITYQVTMIGFTPGFSYLGELPEQLFMPRLAV
                     PKLHLLPGSIGISGYRTGIYPLGGPGGWQIIGCTPMKLFDSELNNPFKLSTGMTIKFS
                     AISIEQFQDHQNQVTR"
     gene            1325..2260
                     /locus_tag="QGH35_05720"
     CDS             1325..2260
                     /locus_tag="QGH35_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HJL82063.1"
                     /translation="MSIEVITPGLQTTIQDEGRSGWRHMGVPQSGAADLFSCKAANYL
                     LGKACDSAVIECTLTGPRLSFLKEMQILITGSDMNPKINDKQLDMYKPYSVNVGDELV
                     MSACSYGCRSYIAFSDDLVCNPIMGSQSTYLPANFGGFNGKILQKGDIIQTISSPNLT
                     SKLQDSAEITGQDFTNQWCLKAIPGPEFEVLDQSSQTLLFSETFKVSNDSDRMGSRLI
                     GKNLNVKTTDHMTSGPLFPGTLQCPKDGSPILLGSDAQTLGGYPRLIQVPLFNQHLIG
                     QLRPGDSIEFRRISNEEAVEELKQQALSFPFIANI"
     gene            2280..2356
                     /locus_tag="QGH35_05725"
     tRNA            2280..2356
                     /locus_tag="QGH35_05725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2314..2316,aa:Arg,seq:tcg)
     gene            complement(2372..3163)
                     /locus_tag="QGH35_05730"
     CDS             complement(2372..3163)
                     /locus_tag="QGH35_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL82064.1"
                     /translation="MTDVDRTVLITGSNRGIGLEFVKQYLLIDWEVIATCRNPEAADD
                     LIQLEKDHSNLTLKSLDITQDESVNDLAAQLKEKPIDLLINNAAYLGPPDPQKFGQID
                     YKMFTKSFEVNAIGPIRVTEKLIENIRLGNMKKIVFLASAAGSNGQIAPPVTLYSYRA
                     SKAALHLAAHNLYHELKSEKIMVSLINPGLVDTRGFLDLKLGDPIPEHLTKIVPVILI
                     QMILEGKIPMITTLESVTQMIRYIDHLTLDSEPFFVNCDGTPIPW"
     gene            complement(3174..4289)
                     /locus_tag="QGH35_05735"
     CDS             complement(3174..4289)
                     /locus_tag="QGH35_05735"
                     /inference="COORDINATES: protein motif:HMM:NF039236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HJL82065.1"
                     /translation="MYTNLWYVAARSSDLKDQPLYVKMLGSDFVLFRDKAGNPACLSN
                     VCPHRGSSLSDGSCSEEGMIVCPFHGWQYDNKGRCRKIPSIGSDDSEKVTPEAKIDHY
                     PAVDKHGLIWTFLGDQPEQAVPIFDIPELDDPSYTYVEYDVVFESNFHLAKFSNLDYV
                     HLPIVHGISFLDQENPVQPPPHEVRRTDYGLTAFIHTESTYSKGEWKNIREKGTQVKS
                     VLKYFLAGQTLRGEVEIGAMGSGTFNCFYEFSTPIDETSTMMRYYFFRNFMTEKENDP
                     IALERNLKNIFEDKDIAEAQVPKGGPDGMSPIVGLHEDTIIKVYWEIMHEMKAKGWQI
                     DQKKWREMIANNNYCVVPSVARKANPEGWSYPEIPRL"
     gene            4396..5706
                     /locus_tag="QGH35_05740"
     CDS             4396..5706
                     /locus_tag="QGH35_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL82066.1"
                     /translation="MNKSFSSDESRKLGPILLMPGISRVSAISFFPVAMLNTSVFAFM
                     NFMQPYVLEEHLGIARNIQGAVVGSISTMQELIIILLTAFMGALADKVGRRPIFSIGL
                     LLMALAYLLFPLSTEFYHLYLIRAIFAVGVSAASTILLVYTGDYPQERSRAKWNGFMG
                     LFQGIGVMATSLVLTKMPMVFKARGFDPIDAGTYTFWIATIICLLASLIVFTLLKPGL
                     KTIKIQKPKLGLLIKEGYTAAKKDSRVRLAFIASLAARGDIFVVGAFTFLWMTRFAID
                     NGMTIDEGYARGAIIVPIIAGTVVITAPLFGFLFDRLHRLYGVMIAFTLSSSGYLMMG
                     LVDDPMSSSIIPTAIFLGMGEGASIISSTSLMGKTAPAAIRGTLFGAFAMCGAIGQII
                     AGGAGGFLFDYIYTGPFLLMACLNFLVLLVAIYTWVSRKKNNLE"
     gene            5794..6357
                     /locus_tag="QGH35_05745"
     CDS             5794..6357
                     /locus_tag="QGH35_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013412.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJL82067.1"
                     /translation="MSNAHPWLEQIPWTTKALPRDMLEHRIQRALTLSNLGMLGTLGL
                     HGPIVSPLEFYADKFTVYIFPQPGSPKLKAMQRDPRVSFAVANPMAGWVVAQGAQLFG
                     KAELLDPGTADWEYGMTIFRWQASSAEIGFGFDQPPQGILMKLDPDRIVYTEHFLRKD
                     GYGPRQIWRKDEKAEQASDGLTESENT"
     gene            complement(6375..7580)
                     /locus_tag="QGH35_05750"
     CDS             complement(6375..7580)
                     /locus_tag="QGH35_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL82068.1"
                     /translation="MNERQSRISLDKIILIIGLIIYGAGFSMLYIIFAPLSRSIGLST
                     NQFGILLAVSNVALVFSSFYWGKKSQSIGRKPVFIIGLFSYALAYALFAFGIQLGLWG
                     LFATWQLFSILFVIRLIYGGLIGGIQPAAVAYISDSTEDKHRTQGMALIGMSSGIGTM
                     IGPALGGGLAFIHPLFPLYFGAALAIIGGLLAMKFLIEPNQRLQAQPVQNHNLKFYDK
                     RIIPYLIGWAVVFLVFTGTQVIAAFFIEDRLGVTGYNEIIKATSIALLSMALATTLMQ
                     AVVLQLINIPVKILLRLSFFIFGLVLLSIPYIENLNYYYLSFAGIGVAFSMISPVLNS
                     AATLSVEQHEQGEMAGLLSAAPVVGMIFGPTIGALLYNINPNYPFFFGALAVIVLGIY
                     FQFVKTPNT"
     gene            complement(7607..>8396)
                     /locus_tag="QGH35_05755"
     CDS             complement(7607..>8396)
                     /locus_tag="QGH35_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82069.1"
                     /translation="DNIRTQALVRTPGVEIGQLHLVEFKTPNDPVRKDANSTDLGPKN
                     LDLSCKDLPKKYDELQQMGYSFRSKYIEYRIETLQADVIEVQMPGHDETNIVFMEWLE
                     EKIKLSAKGYGGITSLVVIVPDTAREGKFYSDLFNLKETLHEELFGEHIEKMIGLPQG
                     GGLNLRLLSGDEKDRYGTVEIISYVGASDQVNRFKLASPPALGVLHCSFLTNSMKAFT
                     QKAKTIGVDLAHFGDLNLIYGNGPVCSLRSPAGLRIDVQERTSK"
BASE COUNT         2492 a         1640 c         1690 g         2574 t
ORIGIN      
        1 atgaaaaaaa tcattgttgc ttgtcagaaa aataaaatat ccattggtgc acacccctcc
       61 tatccagacc agatcagttt tggcagacgc acaattgaga ttacggaatc taaactaaag
      121 gagtcattga ctcatcaaat caataggctc ttagatttat caaaggagct caataccaaa
      181 ctaacccatg tgaaagcaca tggagctctt tatcatgatg tgtcaactag taacaatgtt
      241 gcagcactct ttgttgatgt catcaatcaa attgatccaa atctatatat tttggggcca
      301 ccaggttcgg cgatagagag tgtcgtagaa gagcatagga tgcatttcat ctgcgaagct
      361 tttctagaca gacgatatag tgacagtggg tttctactta atcggagtct gcagggagct
      421 attttagaaa atatatcaga gcgttgtgag caagcagtaa atattgtttg tcatcataaa
      481 gtgcaaacag aaactggagg ttttttcaat cttaaagctg agacactgtg tcttcatagt
      541 gacagtccca atgcagttaa aacggcagcc agggtctgtt cagaacttgc aaaagtaaac
      601 attacaattc aaagctttgt tgaatgaaaa aaattgttaa cccaccaatt atccactacg
      661 gtgacgatgc cttattggta aaaattcaac acttagcatt aacctgtaaa ccgaacgaat
      721 atgctcttgc actgttggcc tatctaagag catccaacaa tgactggatc gatgtcgttc
      781 ccgctgaaga aagtctcgcg gttagataca gcccctttag attgtctgct aaagaagcgt
      841 acagcaactt ggaaaaacag attgatgaat tcgactacca gccgttaacg caaaacaatc
      901 aattaattca tattcctgtt tgttaccacg ataaatttgc accagatatg cacactgtaa
      961 cccagcagaa agtattgagt agggaagagg tcatcaagat ccatagcagc attacatatc
     1021 aagtaaccat gattggattt acacccgggt tttcctatct tggtgaattg ccggagcagt
     1081 tattcatgcc gcgtcttgct gttccaaaat tgcatttatt accaggatca atcggtattt
     1141 caggatatag aacaggaatt tatcccctcg gaggacctgg agggtggcaa attattgggt
     1201 gcacaccaat gaaattattt gattcagaat taaacaatcc atttaagtta tcgactggca
     1261 tgaccattaa attctcagcg atatcgattg aacaatttca agaccatcaa aatcaagtga
     1321 ccagatgagc atcgaagtaa tcacaccagg actgcaaacc acgattcaag atgagggtcg
     1381 aagtggctgg cgtcatatgg gggttccaca atctggtgct gcagaccttt tctcttgtaa
     1441 ggctgccaat tatttgcttg ggaaagcatg tgattctgca gtgatagaat gcacactcac
     1501 aggacccaga ttatcttttc taaaagagat gcaaatttta atcacagggt ctgacatgaa
     1561 tcctaagatc aatgacaagc aactcgatat gtataaaccc tacagtgtta atgttggaga
     1621 tgagctggtg atgagtgctt gttcttatgg atgtcgtagt tatatagctt tctctgatga
     1681 tttagtgtgc aacccaataa tgggtagcca gtcaacctat ctgcctgcta attttggcgg
     1741 tttcaatggg aaaattctgc agaaaggaga catcattcag accataagct cgcctaatct
     1801 cacaagtaaa cttcaggatt ctgctgagat tacaggtcag gattttacta atcaatggtg
     1861 tctcaaagca attccgggac cagagtttga agttctggat caatcttcac aaaccctttt
     1921 gttttctgaa acttttaagg tatccaacga ttcagatcgc atggggagtc gcttaattgg
     1981 taagaatcta aatgttaaaa caaccgatca tatgacaagt ggaccactgt ttcctggtac
     2041 ccttcaatgc cctaaggatg gatctccaat attattgggt tcagacgccc aaacgctggg
     2101 tggctacccg agattgattc aagttccact tttcaatcaa catttaatcg gtcaactaag
     2161 accgggtgac tctatagaat ttagaagaat ttcaaatgag gaagctgttg aagagttaaa
     2221 acaacaagca ttatcatttc cctttattgc taatatataa tgcatgcagt ttcaaagttg
     2281 cgcctgtagc tcagttggat agagtacctg gcttcgaacc aggtggtcgg gggttcgaat
     2341 ccctccaggc gcgccaccaa tttgaatcaa attaccaggg gattggtgtg ccatcacaat
     2401 tgacaaaaaa tggttcacta tccaatgtca gatgatctat atatcgaatc atctgtgtca
     2461 ctgattccag agtcgtaatc attggaatct ttccttctag aatcatttgt atcagtatga
     2521 cggggacaat ttttgttaaa tgttcaggga tgggatcacc cagttttaga tctagaaaac
     2581 ctcgagtatc gactaagcca ggattaatga gggaaaccat aattttttca gacttcaatt
     2641 catgataaag gttatgtgct gccaaatgaa gtgcggcttt gctggctcta taggaataca
     2701 gcgtaactgg gggtgctatc tgaccattag atccggctgc gctagccagg aatacaattt
     2761 ttttcatatt ccctagtcgg atattctcaa ttaatttttc tgtgacacga atgggaccta
     2821 tggcattcac ttcaaagctt tttgtgaaca ttttataatc aatttgtcca aatttttggg
     2881 gatccggtgg cccaagataa gcggcattgt tgatcaataa atcgataggt ttttctttta
     2941 attgagcagc cagatcatta acactttcat cttgggttat atccagtgat tttagtgtaa
     3001 gattagagtg atccttttcc agttggatca aatcatccgc tgcctccgga tttcgacagg
     3061 ttgcgattac ttcccaatca atcaaaagat attgtttgac aaattcaagt ccaatgcctc
     3121 gattggaacc ggtgattaaa actgttctgt ctacatcagt catcgatact cctctatagt
     3181 cttggaattt caggatatga ccacccttct gggttggctt ttcttgcaac agaagggaca
     3241 acacagtagt tgttattggc aatcatttct cgccattttt tttgatcaat ttgccagcct
     3301 tttgctttca tttcatgcat tatttcccag taaactttta taattgtgtc ttcgtgaaga
     3361 ccgacaatag gtgacatacc atcgggacca cccttaggca cttgggcttc agcaatatcc
     3421 ttgtcttcaa aaatgttctt caaatttctc tcaagtgcta tagggtcatt ttctttttca
     3481 gtcataaaat ttctaaagaa ataatagcgc atcattgtgc ttgtttcatc tatcggtgtt
     3541 gagaattcat aaaagcaatt gaatgttcca gagcccattg caccaatttc cacttcacct
     3601 cggagtgttt gtccagctaa aaaatatttt aaaacagatt taacttgtgt tcctttctct
     3661 ctaatatttt tccattcacc tttgctatag gttgattcgg tatgaataaa tgcagtcagc
     3721 ccataatcag ttctcctgac ttcgtgaggg ggtggttgaa ctgggttttc ttgatctaga
     3781 aatgatatgc catgaacaat tggaagatga acatagtcta agttactgaa ctttgccaaa
     3841 tgaaaattag attcaaaaac gacatcatat tcaacatagg tataacttgg atcgtcgagt
     3901 tccggaatgt caaaaattgg taccgcctgt tcaggttgat cccctagaaa agtccagatc
     3961 aatccatgtt tatctactgc gggatagtga tcaattttgg cttcaggggt tactttttca
     4021 gaatcatcac taccgataga aggtattttt ctacagcgtc ccttgttgtc atattgccag
     4081 ccatggaaag gacacactat catcccttcc tcgctacacg aaccatctga caaactcgag
     4141 cctctgtgtg gacaaacatt actcagacag gctgggttgc ctgctttatc tctaaataaa
     4201 acaaaatcag aaccgagcat tttcacatac agaggctgat cctttaaatc actgcttcgt
     4261 gctgccacat accaaagatt ggtgtacatc gtcttaatcg ttatctatat taattaactg
     4321 gtgatcatat tttgtaggac gatcactcat ctacatataa tggtagtata gcaatcaata
     4381 aaaatgaaat gagttatgaa taaaagtttt tcatctgatg aatctagaaa actaggccct
     4441 atattattga tgcccggtat tagtagggtc agcgctatta gtttctttcc agtagcaatg
     4501 ttaaatacct cggtatttgc ttttatgaat ttcatgcaac cctatgtcct agaagagcat
     4561 cttgggattg caagaaacat acaaggggct gttgttggtt caattagcac aatgcaggaa
     4621 ttaataatta tattattaac tgctttcatg ggagcattgg ctgataaggt cggaagacga
     4681 cccatttttt caataggcct cttattgatg gcgcttgctt atttactgtt tcccctatcc
     4741 actgagtttt accatctcta tctcattcga gccatttttg ctgtaggagt atctgccgct
     4801 tcaactatac tgctggttta tacaggcgat tatcctcaag aacgttcaag ggcaaaatgg
     4861 aatggtttca tgggtttatt ccagggaata ggagtgatgg caacgtcgtt agtcttaaca
     4921 aaaatgccaa tggttttcaa agcaaggggg tttgatccta ttgatgctgg aacctatacc
     4981 ttttggatag cgaccattat ttgcttatta gcatccctga tagtttttac tttattgaaa
     5041 ccaggattga agacaataaa aattcagaag cccaaactgg gtttattgat aaaggagggt
     5101 tatacagctg ctaaaaaaga ttctagagtc agattagcct tcattgcttc tctggctgct
     5161 aggggtgata tttttgtggt aggagccttt acatttttgt ggatgactcg ttttgccata
     5221 gataatggca tgaccattga cgagggttat gcccgtggag cgatcattgt gcccatcata
     5281 gctggcacgg tagtaattac cgccccctta tttggtttcc tgtttgatcg attgcataga
     5341 ctttatggag tcatgattgc atttacactg tcatcctcag gttatttaat gatgggtctc
     5401 gttgatgatc cgatgtcatc ctcaataata ccgacagcca ttttcttggg catgggtgaa
     5461 ggcgcatcga ttatttcttc gacatctttg atggggaaaa cagctccggc agcaattaga
     5521 ggaacgcttt ttggtgcttt tgcaatgtgt ggtgccattg gtcaaattat tgccggtgga
     5581 gcgggtggtt ttttgtttga ttatatttat acaggtccat ttttactcat ggcctgtcta
     5641 aattttcttg ttttgctcgt ggccatctat acatgggttt ctcgaaagaa aaataactta
     5701 gaataaagtt caaattacaa tttaattgtt tttccttttc ccaatttcat ttttaatgca
     5761 tgataaaaag ctaatacata ttggaggtta cacatgagta atgcacatcc ttggctggaa
     5821 caaatacctt ggacaaccaa agcattgcct agagacatgt tggagcatcg tattcagaga
     5881 gctcttacat tgagcaatct tggaatgttg ggcacactag gtttacatgg accaatagtg
     5941 agtccgttgg agttctatgc agataaattc accgtttata ttttccccca acctggcagc
     6001 ccaaagctta aagccatgca gagagatcca agggtttcat ttgcagtggc aaaccccatg
     6061 gctggatggg tggtggctca gggtgcacaa ttgtttggca aggcagaatt attggatcct
     6121 ggcacagcag attgggagta tggtatgact atttttagat ggcaggcatc gtcggcagag
     6181 attgggttcg gatttgatca accgcctcaa ggaattctta tgaaattaga tcctgatcgg
     6241 attgtctata ccgagcattt tcttagaaaa gacggttatg ggcctcggca aatatggcgt
     6301 aaggatgaga aggcagaaca agcttctgat ggtttaacag aatcagaaaa tacctaatta
     6361 taaaattaga aaatttaagt attaggagtt ttaacaaatt ggaagtagat tcctaaaaca
     6421 attactgcca aggcaccaaa gaaaaaagga tagtttggat tgatattata gagcagggca
     6481 ccaattgtag gtccaaaaat catgcctact actggtgctg ccgataacag acctgccatt
     6541 tctccttgtt catgttgttc aacacttaag gttgcagctg agtttaatac tggagagatc
     6601 atgctaaaag caacaccaat gccggcaaat gataaataat agtagttcag gttctctatg
     6661 tatggaattg acaacagtac caatccaaaa ataaaaaaac tgagtcgcaa taagattttt
     6721 acaggtatat ttatgagttg taagactaca gcttgcatca gtgttgtggc tagagccatt
     6781 gagagcaacg caatgctggt tgctttaatg atttcattat aacccgtaac accaagtcga
     6841 tcttcaataa agaatgccgc tatgacttgt gttccagtaa ataccaaaaa tacaactgcc
     6901 caaccaataa ggtagggtat gattcgcttg tcataaaatt ttaaattgtg attttgtact
     6961 ggttgagcct gcagtctttg atttggctca attaaaaact tcattgctaa aagaccgcct
     7021 atgattgcca gggctgcacc aaaatacaat ggaaataagg gatgaatgaa agcaagccca
     7081 cctccaaggg caggaccaat catggtacca ataccagacg acataccaat gagtgccatg
     7141 ccttgggttc tatgtttatc ctcggttgaa tctgagatat aggcaaccgc agcgggttgg
     7201 atacctccaa taagaccccc atagattaat ctgatcacaa ataatatgga aaataactgc
     7261 caagtggcaa acaatcccca taagccaagt tgaattccaa atgcaaacaa tgcataagct
     7321 aatgcataac tgaatagacc tataataaaa acaggttttc tgccaattga ctgacttttt
     7381 ttaccccaat aaaaggatga aaataccaaa gccacattag aaacggcaag caatatgcca
     7441 aattgatttg tactcagacc aatgcttcgg gataaaggag caaaaatgat atagagcatt
     7501 gaaaatccgg ctccataaat aatcaagccg ataatcaaaa tgattttgtc tagactaatc
     7561 ctagattgtc tttcattcat tagctgtatt ggagattatt ataatattac ttagaggttc
     7621 tctcttggac atctattctg agtcctgcag gggatcttaa actacaaaca ggaccgtttc
     7681 catatattaa attaagatct ccaaaatggg ctagatcaac ccctattgtt tttgcttttt
     7741 gagtgaatgc tttcatagaa ttggttaaaa aagagcaatg caaaacccct agtgcggggg
     7801 gagaagctaa tttaaaacgg ttcacttgat cggatgctcc tacatagcta atgatttcta
     7861 ctgtcccata tctgtctttt tcatcaccgg agagtagtct tagattcaat cctccccctt
     7921 ggggtaaacc aatcattttc tcgatgtgtt ctccaaataa ctcttcgtgt agagtttctt
     7981 tcaagttgaa tagatccgaa tagaattttc cttccctggc ggtatccgga acgatgacca
     8041 ccaaggaggt gataccacca taaccttttg ccgataattt tattttctct tctagccact
     8101 ccatgaaaac aatatttgtt tcatcgtgac cgggcatttg tacttcaatg acatctgctt
     8161 gtaatgtttc tatcctgtat tcgatgtact tggatctgaa tgaataaccc atttgttgca
     8221 actcatcata tttttttggt aaatctttgc aggaaaggtc cagattcttg ggacccaaat
     8281 cggtgctatt ggcatctttt ctgacgggat cattgggagt cttaaattcc actaagtgaa
     8341 gttgaccaat ttcgactcca ggcgttctga ctaaagcttg agttcttata ttatcg
//
LOCUS       DASCTH010000271         3812 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000597649, whole genome shotgun sequence.
ACCESSION   DASCTH010000271 DASCTH010000000
VERSION     DASCTH010000271.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000597649"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="QGH35_05760"
     CDS             <1..221
                     /locus_tag="QGH35_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82070.1"
                     /translation="RPVGKDRPKHRNEYVKGLWFAGDQYGEKNWGCGVDAAVLSGTVC
                     VDQLMGTNFEEDIFPPHHRGILVESGAD"
     gene            314..751
                     /locus_tag="QGH35_05765"
     CDS             314..751
                     /locus_tag="QGH35_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020471335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HJL82071.1"
                     /translation="MMNTITNFFAVLKLPAGWWKKLVLLGLAAFFINIGVDHFVNPDF
                     YLSIMPPAFPLHSEAVYISGFFEVLGGICVLIPRLRKITGWGLVALLVTVYPANIYMA
                     MTPEAFPDIPVTLLYVRLAFQFIFFYWAYSVTQPAYNLADQKT"
     gene            852..2129
                     /locus_tag="QGH35_05770"
     CDS             852..2129
                     /locus_tag="QGH35_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020033246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HJL82072.1"
                     /translation="MNTYSNYENFKEIRTKTEARRQQENRPHEVLYFHKVDDPYSHLT
                     IQCIEKFKSSFNVIIKPILVGEENPEAVHEPSLYNIYCLEDVKRIALFYNIDFPGDDY
                     PSIKLIDKANSILTAVKDENFGEIAKLVSFALWRGDHEALNDLSSKYRSTKSEVKETL
                     SCGNETRNDRGYYFGSAFYYEKELYWGVDRLHYLEERLIELGLKNNSENESVCPLELK
                     APTQLSSEKKVNLFYYPSLNSPYTFISAKRVRKIKDEYPINLITQPVLPMLMRMMTIP
                     DFQARYIISDAAREGRKHGHEIKSIYSPIGKPARKSYSLFPIINEAGKGFEYIDALLK
                     ASFQDGINIGDETFLERLVIKLGLDWSVIKKDLNTKHWKKVLNNNLQDMYAGNCWGVP
                     SFKVTDLDGNNPFYVWGQDRMWLIKEEIAKRLS"
     gene            complement(2130..2873)
                     /locus_tag="QGH35_05775"
     CDS             complement(2130..2873)
                     /locus_tag="QGH35_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012334.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJL82073.1"
                     /translation="MKSNQKVFITGASSGIGAAIAAEYASQGAILGLVARREAKLEAI
                     KNKCLELGAEDVKTYSLDVTDMEQSMSIAKDFIAWAKEGIDIVVANAGVAFSDHLSSG
                     DPTQINQTLLINVLGVTNTVIPFVPTMKSQKKGAIVIMSSIASFTAPAYFGGYSASKV
                     AVRRLGDGWRKTLQKHNVQVTTICPGYIKSEMTDLNDFKMPFLMETDVAAKKMVQAIK
                     SRKKTYIVPWQWRPVIAISRFFGRKLKII"
     gene            2981..>3812
                     /locus_tag="QGH35_05780"
     CDS             2981..>3812
                     /locus_tag="QGH35_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012575.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HJL82074.1"
                     /translation="MKKLKSDTEVIRQIFQHIDAGTTDLGESDWKEPVENYTSEKRFD
                     AELNLLKCLPVAFAPSAALLDEGSYIARPVAGVPIIVVRDKSKQLRAFRNACRHRGKT
                     LVEGEGKTSVFQCRYHGWTYGIDGSLQYVPHEAGFPGLDHGCHGLMPIHGVIEQGGLV
                     YVCIEEPFDQGALGNMPQIIKDDQKIFDSSEYSQAFNWKLNIEATMEGYHIKPTHEKT
                     FYPYGYDNLNIVETFGLNSRITFPFRRIEDLRNIAEEKRDITGKVTYVYSVFPNCTVA
                     F"
BASE COUNT         1168 a          715 c          788 g         1141 t
ORIGIN      
        1 cgcgaccagt aggaaaagat cgccctaaac atagaaatga gtatgttaaa gggttgtggt
       61 ttgcgggtga tcagtacgga gagaagaact ggggttgcgg ggttgatgcg gctgtactat
      121 ccgggacagt atgtgtcgac caattgatgg ggacaaattt tgaggaggat atctttcccc
      181 cccatcaccg agggattctg gtggaatcag gggcagacta gagctattct aaagtaggag
      241 attttcgaga ttcaccatga aatcaactcc gcagttaatt acggcggttg cggttgcact
      301 tgtatgacta aaaatgatga acactataac taattttttt gctgtactta aactacctgc
      361 cggttggtgg aaaaagttgg ttctattagg gttggctgct ttttttatca atataggagt
      421 agatcatttt gttaatccag acttttattt atccattatg cctccggcgt ttccattgca
      481 ttctgaggct gtttatatca gtggcttttt tgaagtactg ggaggcatct gtgttttgat
      541 tcctcgcctg cgtaaaataa ctggctgggg attagttgct ctgctagtta ccgtttatcc
      601 ggctaatatt tatatggcta tgacacctga ggcttttcct gatatccctg ttacactgct
      661 ttatgttcgt ctagcattcc agtttatatt tttctattgg gcttactcag taacgcagcc
      721 tgcctataac ttagctgatc aaaagacgta atctagttct taaatcagtg aacagacaat
      781 agattatgct caaagctttt agacatttca tggtcaggcg cacgatcatg gccaagatca
      841 ggaatcatgc aatgaacacg tattccaact atgaaaattt taaagaaatt cgaacaaaaa
      901 ctgaagctag aagacaacaa gaaaatagac cacacgaagt gttatatttt cataaagtag
      961 atgaccccta ttctcatctt acaattcaat gtattgagaa atttaaatca tctttcaacg
     1021 ttatcattaa accgattctg gttggtgaag aaaatccaga ggcggtccac gagccctctt
     1081 tgtataatat ctattgttta gaagacgtta agcggattgc gctcttttac aatattgatt
     1141 tccctggtga tgattaccca tcaataaaac taatagataa agcaaattca attttaactg
     1201 cagttaagga tgaaaatttt ggtgaaatag caaaactcgt aagctttgct ttgtggcgag
     1261 gagatcatga agcgctgaat gatttgtctt ccaaataccg ctccaccaag agtgaggtca
     1321 aagagacatt aagctgcggc aacgaaacta gaaacgacag gggttattat tttggttcgg
     1381 cattttatta cgaaaaagaa ttatattggg gcgtggatcg tctgcattat ttagaagaaa
     1441 gactgataga attgggttta aagaataact cagaaaatga atctgtttgt cctttagagt
     1501 tgaaagcccc aacccaactg tcctcagaaa aaaaagttaa cctcttttac tacccatctc
     1561 ttaatagtcc ttatacattt attagtgcca aacgagtaag aaaaataaag gatgaatacc
     1621 ctattaactt aataacacaa cctgttttgc cgatgttaat gcgtatgatg acgattcctg
     1681 acttccaagc tagatacatt atttcagatg ctgctcgtga aggacgaaaa catggccatg
     1741 aaataaaatc tatttattca cccattggga aaccggcaag aaaatcttat tctttattcc
     1801 caattattaa tgaggctgga aaagggtttg aatacattga cgcattacta aaagcttctt
     1861 tccaagatgg aatcaacatt ggtgacgaaa ctttcttgga gagattggta attaaacttg
     1921 ggcttgattg gtcggtcata aaaaaagact taaatacgaa acattggaaa aaggttttaa
     1981 acaataattt acaagacatg tatgcaggaa attgctgggg cgtcccaagt tttaaagtta
     2041 ccgatcttga tggaaataat cctttttacg tttgggggca agatcgaatg tggcttatta
     2101 aggaagaaat tgctaaacgc ttaagctaac tagataattt ttagttttct tccgaaaaat
     2161 cttgagatgg caatcactgg acgccattgc caaggcacga tatacgtttt tttcctagat
     2221 ttaattgctt gcaccatctt tttagcagcc acatcagttt ccataaggaa gggcatttta
     2281 aagtcattaa gatccgtcat ctcgcttttg atatagccag ggcatatcgt ggtgacttgt
     2341 acattatgtt tttgaagtgt ttttctccat ccatctccca atcttctgac tgcaactttt
     2401 gaagcagaat accccccaaa ataagctggc gcggtaaagc tggctataga cgacataata
     2461 acaatggcgc cctttttttg tgatttcatt gttggcacaa aaggaataac agtatttgtt
     2521 acaccgagta cattgataag tagggtttga ttaatttgag ttggatcacc agatgagaga
     2581 tgatctgaga aagcaacacc tgcattagca acaacaatat caatcccttc ctttgcccat
     2641 gcaataaaat cctttgcaat agacattgat tgctccatat cagtaacatc aagtgaatag
     2701 gttttaacat cctcagcacc cagctctaaa catttgtttt taatagcttc aagtttcgct
     2761 tctcttctag caaccaaacc cagaattgct ccttggctag catactctgc agcgattgct
     2821 gccccaatgc cactgctggc tccagtaata aatactttct gatttgattt catagcctta
     2881 gttccttttg atttaggaga tttaatttat cttaattatt actcatttgc tataggatta
     2941 ggtaccctga gaaagagatc aataaaatta caaactagag atgaaaaagc taaaatcaga
     3001 cacagaggta atacgccaga ttttccagca catcgatgca ggtacaaccg atcttggcga
     3061 atctgactgg aaggagcccg tcgaaaatta tacaagcgaa aaaagatttg acgcagaatt
     3121 aaatctctta aagtgtttgc ccgtcgcatt tgcaccctct gcagcactat tggatgaagg
     3181 atcatatatt gcgcgccctg tggcaggggt ccctatcatc gtcgtgcgag ataaaagcaa
     3241 gcaactgagg gcatttcgta atgcatgtcg acatcgtggt aagacgttgg tagaaggaga
     3301 agggaaaaca tctgtttttc aatgtaggta tcacggatgg acctatggta ttgatggttc
     3361 gctgcagtat gttcctcatg aggctggatt tccaggcctg gatcacggct gccatggtct
     3421 tatgccaata catggagtca ttgaacaagg tggtttggtt tatgtgtgca tcgaagagcc
     3481 tttcgatcag ggggccttag ggaatatgcc acaaattatc aaagacgatc aaaaaatctt
     3541 tgattccagt gaatactcgc aggcgttcaa ttggaaactg aacattgaag cgacgatgga
     3601 gggataccac atcaaaccga cccatgaaaa aacattttat ccctatggct acgataacct
     3661 gaatattgtt gagacatttg gtttaaacag cagaatcact ttcccattca ggcgcataga
     3721 agatcttagg aatatagcag aggaaaaaag agatatcacc ggtaaagtaa cctacgttta
     3781 tagcgtgttc ccaaactgta ctgttgcgtt tt
//
LOCUS       DASCTH010000272         5371 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000598476, whole genome shotgun sequence.
ACCESSION   DASCTH010000272 DASCTH010000000
VERSION     DASCTH010000272.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000598476"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="QGH35_05785"
     CDS             <1..987
                     /locus_tag="QGH35_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016274.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HJL82075.1"
                     /translation="HSHCWMMWPTGLDLEYYPNTDLMRVAHAKTANLISNFEPVTIIA
                     NEKDVDDIKNRTEKNVTIMSATIDDSWCRDSGPTFITDGNELAGIDWTFNNYGEFVGT
                     DYKHDAEIAEMIIKHLDIKHFKPPIILEGGAIHTDGKGTLMLTEDVLLDPNRNPGLDK
                     KEAEFILSKYLGVENFIWLIAAMEYDDTGGHVDNLACFTPSNQIITLIEKNKEDSHYE
                     RLRENENRLKHAKNVRGKNYEIIPIQQPKYKEFLNHRMALSYINFYIANGGIVLPIFN
                     DPMDEHAIKTITDVFHDKTVVTLDGTNIVEGGGCVHCITQQQPIINYQQDSS"
     gene            984..1841
                     /gene="aguB"
                     /locus_tag="QGH35_05790"
     CDS             984..1841
                     /gene="aguB"
                     /locus_tag="QGH35_05790"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HJL82076.1"
                     /translation="MRKVTVAATQMPCTNNSEDNIDNAIGLIRDAAAQGANIILIQEL
                     FSSLYFPCLNDPDNFNLAVPLQNNPLIATMANLAKELNVVLPISFFEKDQNTYFNSLV
                     VIDSNGTIMDLYRKSHIPDGPGYEEKYYFHPGNTGFKVWETSFGKIGVGICWDQWFPE
                     AARIMTLSGAELIFYPTAIGSEPDSANFDSKEAWQIVMQGHAAANSIPIIASNRIGVE
                     KNKKFSMTFYGSSFIADECGQKIKECSRDQQEFIIHEFDLDAVELYRSSWGFFRDRRT
                     DLYKDIFRD"
     gene            1850..2749
                     /locus_tag="QGH35_05795"
     CDS             1850..2749
                     /locus_tag="QGH35_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HJL82077.1"
                     /translation="MDTSNKELLIKAASIASLFVSSTLIIIKYFGWTETDSISILGSL
                     ADSLIDFLASIFVFIAISFSSLPADKEHRFGYGKSEGLVVFIQSLLIGVSGLYVFSEA
                     IQRFFHPSEINQPSVAVWIIVVSIVLSIALVSFQKYVVNISQSKAIESDQYHYLTDLY
                     INISVLISILISGWTSYLFVDSLIGLFIAIFVIYSAINLLKKSFQILLDQELPSSERD
                     QIKAIALTHPEVLGFHDLRTRDAGRNYVIQFHLELDPQMTLLHAHEIIEQVMEQVLDA
                     FPDAEIIVHSDPLGIEEKRDPFE"
     gene            2797..3411
                     /locus_tag="QGH35_05800"
     CDS             2797..3411
                     /locus_tag="QGH35_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HJL82078.1"
                     /translation="MKITLRSITLISLIISFITSPNISASKVDEIDAAIDSALERFTD
                     EIQGGATYLEGARGVLVIPKMIKAGVILGMEFGEGALIVDEIKVQYYRAFTTSLGLQV
                     GIGRKDLVILFFDDAAMDEFLYSSGWEIGVDGSVALFTMGAGGSADTITRKDPIVGFV
                     FGHKGLMAGVSLEGTKFSKSWPNSDINEDQEQLDIDEFNEENIE"
     gene            3423..4421
                     /locus_tag="QGH35_05805"
     CDS             3423..4421
                     /locus_tag="QGH35_05805"
                     /inference="COORDINATES: protein motif:HMM:NF020468.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1838 family protein"
                     /protein_id="HJL82079.1"
                     /translation="MFTSLFNSTRSRRYILQLPAIITAASFSPSLAPKDITNNSELDF
                     EDPLDNLYAFGKLWGSYDSKPTYGGYQGVLFARIGTRRLIPLFGYVGCGNMQSRLNAD
                     KTINIRGTEAGYFTDLTSGKIIDDWMNPWTNEKVKVFPFINKAMRAHLTTEMPRFAMG
                     NTATDTPTLMNQTAAQKRSGTVPFILPWQQLGDQYLLTWEYSHEYTNPVTKDKWPKAH
                     TTTTINPSEHFSFYTPIGEMNNKSIPSSPFHAGFMRTSPWWPWMKMGQHQIDGCLFGR
                     MHSFQITGTTDDIPRVVLERVEKDHPEILEKQHGWGTTNPKGTWETYAEEVSPEII"
     gene            4455..5042
                     /locus_tag="QGH35_05810"
     CDS             4455..5042
                     /locus_tag="QGH35_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82080.1"
                     /translation="MNHNAKLLILFVLCCQGCSQSIVVNTIIPDPLVSKIENYNIAVV
                     YGPDIKSFHFEDTDIEPSDITLSIDFQDSQMLTFSKILNGFFHNLTTVDSGNKEIING
                     IDLYLEIKLEAIEYLSPIQSGSEKHAIWFNYKIKIFDHQRTPTTSWDITGYGEDTIGS
                     LKSTQSLEKAVNSGLRDVGVNLSMKIEDDFQKLIE"
     gene            5047..>5371
                     /locus_tag="QGH35_05815"
     CDS             5047..>5371
                     /locus_tag="QGH35_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82081.1"
                     /translation="MMNNPFLFALFCFMAFFISSCTTSRLEEQVNLPFNLSEGEAVVI
                     IANSYHSGNQTEYDFIQCINKSLSKKQNSFNVLPTEDFQNMLYPWLEPRTAPQTPEDL
                     PQLLSR"
BASE COUNT         1790 a          944 c          989 g         1648 t
ORIGIN      
        1 cattcacact gttggatgat gtggccaaca gggcttgatt tagaatacta ccctaacact
       61 gatttgatga gagttgccca tgctaaaaca gctaatctta tttccaactt tgaaccagta
      121 acaataatcg ccaatgaaaa agatgttgat gatataaaaa atagaactga aaaaaatgta
      181 acaattatgt cagctacgat agacgatagt tggtgtaggg attcgggtcc tacgtttata
      241 actgatggta atgaacttgc tggtattgac tggacattta ataattatgg tgaatttgtt
      301 ggtacagatt ataaacatga cgctgaaata gctgagatga ttattaaaca ccttgatatt
      361 aaacatttta aaccccccat aatattagaa ggtggagcaa tccatactga tggtaaagga
      421 accttaatgc ttacagaaga tgtcttattg gatccgaatc ggaatcccgg attagataaa
      481 aaagaagctg aatttatttt gtctaaatat ttaggtgttg aaaattttat ttggcttatt
      541 gcagccatgg aatatgatga caccggtggt catgtagata accttgcatg ttttacgccc
      601 agtaatcaaa taattactct gatagagaaa aacaaagagg attcacatta cgagcgactt
      661 cgagagaatg agaatcgatt aaagcatgcc aaaaatgttc gaggaaaaaa ttatgaaatt
      721 attccaatcc aacaacctaa atataaggaa ttcttaaatc acaggatggc actttcttat
      781 attaatttct atatagctaa tggtggcatt gtcttaccaa tattcaatga cccaatggat
      841 gaacatgcta ttaaaactat caccgatgtt ttccacgata aaacagttgt tacactagat
      901 gggacaaaca ttgtagaagg tggtggttgt gtccattgta ttactcaaca acaacctatt
      961 attaattatc aacaggattc atcatgagaa aagtaacggt tgctgcaaca caaatgccct
     1021 gtactaataa ttctgaggat aatattgaca acgcgatcgg tttaataaga gacgcagctg
     1081 cccaaggagc aaatataatc ctaattcaag aattattttc atctctttat ttcccttgtc
     1141 tgaatgatcc tgataatttt aatttagcag tgccattgca aaataatcca ttgatagcaa
     1201 ccatggcaaa ccttgccaaa gaattgaatg tcgtattgcc cattagtttt tttgaaaaag
     1261 atcaaaatac ttatttcaac tccttggttg ttattgattc aaatggaaca ataatggatc
     1321 tgtatagaaa atcgcatatc ccagatggcc caggttatga agaaaaatat tattttcacc
     1381 ctggaaacac aggatttaaa gtttgggaaa cctcatttgg caagattgga gttggtattt
     1441 gttgggacca atggtttcct gaagctgcga gaataatgac tttatcgggt gcagagttaa
     1501 ttttctaccc aactgcaatt ggaagtgagc cagattctgc taattttgat tccaaagaag
     1561 cctggcaaat cgttatgcaa ggtcacgctg cagcaaatag cataccgatc atcgcctcca
     1621 atagaatcgg agttgaaaaa aataaaaaat tctccatgac cttttatggt tcttctttca
     1681 ttgcagatga gtgtggtcaa aagatcaaag aatgctcaag agaccaacaa gaattcatca
     1741 ttcatgaatt tgacttagat gcagttgaac tttacaggtc atcctgggga ttttttagag
     1801 atcgaaggac agatttgtac aaagatattt ttagagatta aacaaagaaa tggatacatc
     1861 aaataaagaa ctgctgatca aagctgcctc aattgcatcc ttatttgttt catcaactct
     1921 gattattatc aagtattttg gttggacaga aacagactcc atttcaatat taggatctct
     1981 agctgattca ttaatcgatt tcttagcctc tattttcgtg ttcattgcta tcagtttttc
     2041 atcactacca gcagacaaag aacatcgctt tggttatggt aaatcagaag gattggttgt
     2101 ttttattcaa tccttgctaa taggtgtctc cggactatat gttttcagtg aggccataca
     2161 aagattcttc catcctagtg aaattaacca acccagtgtt gctgtttgga tcattgttgt
     2221 atcaattgta ttgagtatag ctttggtttc attccaaaag tacgttgtaa atataagtca
     2281 gtcaaaagcg atagaatcag atcaatacca ctatttaaca gacttatata tcaacatttc
     2341 tgttttaatt tcaattctga tttcaggttg gacttcttat ttatttgttg attcactgat
     2401 tggattattc attgccatat ttgtgatata cagtgcaatc aatttattaa aaaaatcatt
     2461 tcaaatactt ctagatcaag aacttccatc ttctgaacgt gatcaaataa aagccattgc
     2521 attaacacat cctgaagtgc ttggctttca tgacttaaga actcgagatg cagggagaaa
     2581 ctatgtcatc caatttcatc tagagttaga tccccagatg actttattac atgcacatga
     2641 gattattgag caagtcatgg aacaagtcct tgatgccttt cctgatgcag agattattgt
     2701 tcactctgat cctttgggca ttgaagaaaa aagagatcca tttgagtaaa ttggagtaaa
     2761 aagctaatat acacaatcat ccttgaagga aaaataatga aaatcaccct gagatctatc
     2821 accttaatat cattaatcat tagcttcata acatcgccca atatcagtgc cagtaaagtt
     2881 gatgaaattg atgctgccat tgatagcgcc ctagagagat ttaccgatga aattcaaggt
     2941 ggtgcaacct atttagaagg agcaagggga gttttggtta ttccaaaaat gattaaagca
     3001 ggagttatcc taggaatgga atttggtgag ggagcattaa ttgttgatga gataaaagta
     3061 caatactatc gagcatttac aacttctctt ggtctccaag ttggcattgg acgcaaagac
     3121 ctggttatcc tgttctttga tgatgctgcg atggatgaat tcctgtacag ttctggttgg
     3181 gagatcggcg tagacggctc tgtagcctta tttacaatgg gagctggagg atcagcggat
     3241 accattacca gaaaggaccc tattgtaggc tttgtatttg gtcacaaagg tttgatggca
     3301 ggtgtaagtt tagaaggaac aaaattttca aaaagttggc ctaattctga tatcaatgaa
     3361 gatcaagagc aactggatat tgatgaattt aatgaagaaa atatagaata aatgagccct
     3421 ttgtgttcac ttcgctcttt aactcaacca gatctagaag atatatttta caactgccag
     3481 ccataatcac tgctgcctct ttctcaccat cactagctcc taaagacatc actaataata
     3541 gtgagctgga ttttgaagac cctttagaca atttatatgc ttttgggaaa ctatggggtt
     3601 cttatgatag caagccaaca tatggaggtt atcaaggtgt tctatttgca agaatcggta
     3661 ctcgcagact aattccatta tttggttatg ttggatgtgg aaatatgcaa tcccgtctaa
     3721 atgctgataa aacaataaat attagaggaa ccgaagcggg ttattttaca gatttgacaa
     3781 gtggaaaaat tatcgatgat tggatgaatc catggaccaa tgaaaaagtc aaagttttcc
     3841 cttttattaa taaagctatg agagcccatc tcaccactga gatgcccagg tttgcaatgg
     3901 ggaatacagc tacggacact ccaacattga tgaatcagac cgctgcacag aaacgctcag
     3961 gaacggtgcc ttttatcctt ccttggcagc aattggggga tcaatactta ttaacatggg
     4021 aatactctca tgaatatacg aatcctgtca caaaagataa atggcccaag gcacacacca
     4081 caacaaccat taatccttcc gagcattttt cattttatac acctattggt gaaatgaata
     4141 ataaatcaat accatcctct ccttttcatg caggatttat gagaacatct ccttggtggc
     4201 catggatgaa aatgggacaa catcaaatcg atggatgttt gtttggtaga atgcatagct
     4261 tccagattac tggaactact gacgacattc caagggtggt gcttgagaga gtagaaaaag
     4321 accaccctga aatccttgaa aaacaacatg gctggggcac tacgaatcct aaaggcactt
     4381 gggagacata tgcggaagaa gtttcacctg agataatata aatagaagat taaacttaat
     4441 ataggatttc cttaatgaat cacaatgcta aattactaat cttatttgtt ctctgttgcc
     4501 aaggttgttc gcaatcaatt gtagttaata caataatacc tgatccacta gtttccaaaa
     4561 ttgagaacta taacattgct gttgtctacg gccctgatat taaaagtttt cattttgaag
     4621 ataccgatat tgaacccagt gacattactt taagtatcga cttccaagat agtcaaatgc
     4681 ttactttttc aaaaattctt aatggttttt tccacaactt aactactgta gacagtggca
     4741 ataaggaaat aatcaatggg attgacctct atcttgaaat taaattagaa gccattgaat
     4801 acctaagtcc tatacagtct ggaagtgaaa aacatgccat ctggttcaat tataaaatca
     4861 agatctttga tcatcaaaga actccaacaa catcctggga tataacgggt tacggtgaag
     4921 atacaatagg atccctcaaa tctactcaat cattagaaaa agcagttaat tctggattaa
     4981 gagatgttgg tgtgaactta agcatgaaaa tcgaagatga ttttcaaaaa ttgatagaat
     5041 aattatatga tgaataaccc ttttctattt gctctctttt gctttatggc tttttttata
     5101 agcagctgca caacttctcg tcttgaagaa caagtcaatc ttccttttaa tctcagtgaa
     5161 ggtgaagcgg ttgttatcat tgcaaatagt taccattcag gaaatcaaac tgaatacgac
     5221 tttattcaat gtattaataa atctctttca aaaaaacaaa atagctttaa cgttctccca
     5281 acagaagatt tccaaaatat gctttaccca tggcttgaac cgcgaactgc cccacaaaca
     5341 ccagaagact tacctcaatt attgagtaga g
//
LOCUS       DASCTH010000273         3003 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000609964, whole genome shotgun sequence.
ACCESSION   DASCTH010000273 DASCTH010000000
VERSION     DASCTH010000273.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000609964"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /gene="sppA"
                     /locus_tag="QGH35_05820"
     CDS             complement(<1..1451)
                     /gene="sppA"
                     /locus_tag="QGH35_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HJL82082.1"
                     /translation="MSIKTVFSRLGTFLDSTFVFLKRAALVVILIIIIGAIIGGLTGS
                     KVDIPEDAILVLDINGPIVEELSQTEFERTLGQLTDSVVPEVLLSDLIKVIESAKDDD
                     RIKYLLLDLEKFGGASPSKLQAVASTLEDFKTSEKKIIAYSSYGYTTGSYYLAAHADE
                     VYLHDYSELIIDGYRSFRVYFKSFFEKFYVDANVFRCETCEYKAFVEPFFRDDMSDEA
                     KQNIVEWISDLWDTYLSEVSTARGMDVNALKDYIDSPENFLKLTEGDAAQAALDAGLV
                     DKLVNEREFRDYLVSLSGDEEDDSINTIWMNDYQNAAELAHNPYSGLQENNVGLIIAS
                     GSIIDGDGGPGEIAGNDFISLIRKAYFDESVKALVLRVDSGGGSAYASEVIADELERF
                     KASGRPIVASMSSVAASGGYYISAPADKIFANPTTITGSIGVGGFLPTFERTFDQIGL
                     HEDGYSTVDLTTSVMQTLSDREKEIIQMAVDNI"
     gene            complement(1474..2559)
                     /locus_tag="QGH35_05825"
     CDS             complement(1474..2559)
                     /locus_tag="QGH35_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HJL82083.1"
                     /translation="MSKFLHTLAISIAFLITFSSTSAFQMPDIDLPEGFSIKVLTDQV
                     PGARSMALGDKGTLFVSTRRAGKVYAVSGIYSEPEVLTIASGLYMPNGVAFKDGNLYV
                     AEIHRVHKFEDIENRLNAPKSSIINKSYPTDRHHGWRYITFGPDGKLYIPIGAPCNIC
                     DEPDYGVITRIDTDGSNKEVIAQGIRNTVGITFHPKTNDLWFTDNNKDMMGDDIPPGE
                     LNRLSKEGEHFGFPFCHASNIPDNEGFDSLGNCSDITPPVQELDAHVAALGLTFYTGT
                     MFPDEYKNQIFIAEHGSWNRSKKSGYRITLVRLDEDRAVSYEVFADGWMKEEKTLGRP
                     VDLLVLPDGSMLLSDDDNGVIYQITYQ"
     gene            complement(2566..2910)
                     /gene="rplS"
                     /locus_tag="QGH35_05830"
     CDS             complement(2566..2910)
                     /gene="rplS"
                     /locus_tag="QGH35_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014147258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HJL82084.1"
                     /translation="MTNLIEDIEKQQLRDDIPEFNSGDTVAVQVRIREGGRERLQIFE
                     GIVIAKKNRGLNSSFTVRKISNGEGVERVFQTHSKLITEIKVKRRGDVRRAKLYYLRE
                     RSGRSARIKEKV"
BASE COUNT          896 a          673 c          488 g          946 t
ORIGIN      
        1 taaatattat caaccgccat ttggattatt tctttttccc tatctgataa agtttgcatt
       61 acagaggtag tcagatcgac tgttgaataa ccgtcctcat gaagcccaat ttgatcaaaa
      121 gttctctcga aggtaggtaa aaaccctccc acacctattg atcctgtaat agtcgtagga
      181 tttgcaaaga ttttatctgc cggtgcagag atataataac cacccgatgc agcaacactg
      241 gacattgaag caacaatagg tcgaccgctt gctttgaatc tttccagctc gtcagcaatt
      301 acctctgatg cgtaagcgct ccctcctcca ctatcgactc gtaataccaa agctttgact
      361 gattcgtcaa aataagcctt gcggataagc gatataaaat cattaccagc aatttctcca
      421 ggtccaccgt cgccatcaat aatgctgcca gaggcaataa tgagtcccac attattctct
      481 tgcaatccac tataaggatt atgagcaagt tcggcagcat tctggtagtc attcatccaa
      541 atagtattta tcgaatcatc ctcttcatca cctgaaagag aaacaaggta gtctctaaac
      601 tctctttcat taaccagttt atctaccaat ccagcatcca aggctgcctg ggctgcatca
      661 ccttcggtga gttttaaaaa attttctggt gaatcgatat aatctttcag agcattcaca
      721 tccatacctc ttgccgtgct gacttcactc agataggtat cccatagatc tgaaatccat
      781 tcgacaatat tttgttttgc ctcatcagac atgtcatccc taaaaaaagg ctctacaaaa
      841 gctttgtatt cgcacgtctc gcaacgaaag acattggcat ctacataaaa tttttcgaaa
      901 aaagatttga aataaactct aaaggatctg tatccatcga ttatcagttc tgaataatca
      961 tgtaaatata cctcatcagc atgtgctgca agataataag aacctgttgt ataaccatag
     1021 ctgctgtaag caattatctt cttctcagaa gttttaaaat cctccaaagt actagccacg
     1081 gcctgcaatt tggatggact ggcgccacca aatttttcta gatcaagtag caaatattta
     1141 attctgtcat cgtcttttgc tgattcaatc actttgatta gatccgataa aagaacctct
     1201 ggaaccactg aatccgtcaa ttgacccagt gtcctctcaa attctgtttg tgatagttcc
     1261 tcaacaattg gtccattgat atcaagcact aatattgcat cttctggaat gtcgaccttt
     1321 gatccagtca gacctcctat aattgctccg ataataataa tcaaaataac tacaagagca
     1381 gcccttttta aaaagacaaa tgtgctgtcc aaaaatgtcc ctaatctact gaatacggtt
     1441 ttaatgctca ttaaatatga ctcctaataa actttactga taagtaattt ggtatatcac
     1501 gccattatcg tcatcgctta acagcatgga accatcaggc aaaactaata aatcaactgg
     1561 tctgcctaag gttttttcct ctttcatcca accatctgcg aatacttcat atgaaacagc
     1621 tcgatcctca tccaatctaa ccaaggtaat ccggtaaccg ctcttctttg atcgattcca
     1681 agaaccatgt tctgctataa atatttggtt tttatattca tctggaaaca tggtgcctgt
     1741 ataaaaagtc aggccaagag cagcaacatg agcatctagt tcctggacag gaggcgttat
     1801 gtcactacaa ttcccaagac tgtcaaatcc ctcattatca ggaatattac tcgcatggca
     1861 aaaagggaaa ccaaaatgct ctccttcctt actcaatcgg ttaagttcgc caggaggaat
     1921 atcatctccc atcatatcct tgttgttgtc ggtgaaccag aggtcgtttg tttttggatg
     1981 aaaagttata ccaacggtat ttctaatgcc ttgggcaatt acttctttgt tagaaccatc
     2041 tgtgtcaatc ctagtaataa ccccataatc tggttcgtca caaatattgc agggcgcacc
     2101 gataggaata tagagtttgc catccggtcc aaacgttata taacgccagc cgtgatgacg
     2161 atcggttgga tacgatttgt tgataatgct ggatttaggt gcatttaatc gattctctat
     2221 atcttcaaat ttgtgaactc tgtgaatttc agctacataa agattaccgt ctttaaaagc
     2281 aaccccgttg ggcatataca ggcccgaggc aatggtcagt acctctggtt ctgaatagat
     2341 accagaaacg gcatacacct ttcctgctct ccttgttgaa acaaataaag tacctttatc
     2401 ccctaaagcc atggatcttg caccaggaac ttgatcagtt agaaccttaa ttgaaaaacc
     2461 ttcaggcaaa tcaatatctg gcatttgaaa tgctgatgta gatgaaaagg tgataagaaa
     2521 tgcaatagaa attgctaacg tatgaagaaa tttactcatg ctgtattaaa ctttttcttt
     2581 aattcttgcg gatctgccac ttctctccct gagatagtaa agcttagccc gcctaacatc
     2641 accacgtctc tttactttta tttcagtaat gagcttacta tgggtttgaa acactctttc
     2701 aacgccctct ccattagata tttttctgac ggtaaaggat gagtttagtc ctctattttt
     2761 ctttgctata acgatgcctt caaagatttg taatctttct cgtccacctt ctctaattct
     2821 aacttgaaca gctacggtat ctcctgagtt aaactcaggg atatcatctc ttaattgttg
     2881 tttctctata tcttcaatta aattagtcat tctgttacac cgtctaaatt gagccagcat
     2941 tatatattaa attaactatt tttagttaac tcttattttc taattcctct tgatattctt
     3001 gaa
//
LOCUS       DASCTH010000274         3482 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000613186, whole genome shotgun sequence.
ACCESSION   DASCTH010000274 DASCTH010000000
VERSION     DASCTH010000274.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000613186"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="QGH35_05835"
     CDS             complement(<1..270)
                     /locus_tag="QGH35_05835"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HJL82085.1"
                     /translation="MNSNELNSIAQAMVAPNKGILAADESTPTIGKRFKDIDTESTEI
                     NRQIYRNMLFTSAGISDYISGVILFEETLRQSNDTGVAFPEYLNSL"
     gene            417..878
                     /locus_tag="QGH35_05840"
     CDS             417..878
                     /locus_tag="QGH35_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HJL82086.1"
                     /translation="MLRINKMTDYGMLILGNLAVSHGGYLSAKDIASSTHISLPTVQK
                     LLKKLNKNNLVVSKQGPLGGYALDKATKLTSVSKILEALEGDLSLMECSAKQSTCEIE
                     NSCQIGNAWQVINQTIFSSLDKLTLLDLIKPSTIEKYIPTEIVIHQNPKRQ"
     gene            882..2342
                     /gene="sufB"
                     /locus_tag="QGH35_05845"
     CDS             882..2342
                     /gene="sufB"
                     /locus_tag="QGH35_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HJL82087.1"
                     /translation="MESNKRKKNAPELEEFIDKKYQYGFVTDIESETLPPGLDENVIR
                     LISEKKNEPEFLLEWRLKAFKFWQKLKPPMWGHLKIDDINYQAISYFSKPKQENAPQS
                     LEDVDPKLLDTYEKLGIPLHERAALAGVAVDAVFDSVSVTTTFKEKLAEAGVIFCPFS
                     EAVRQYPDLIKKYLGKVVPYRDNFYAALNAAVFTDGSFVYIPKGVRCPMELSTYFRIN
                     EAKTGQFERTLIIADEGSYVSYLEGCTAPMRDENQLHAAVVELIAHDDAEIKYSTVQN
                     WYPGDESGQGGIYNFVTKRGDCRGHNSKISWTQIETGSAITWKYPSCILRGDKSVGEF
                     YSIALTNNFQQADTGTKMIHIGKNTKSKVISKGISAGHSQNSYRGLVAVSKNAEASRN
                     YTNCDSLLIGADCGAHTFPSMEIHNSSTILEHEASASKVSDEQLFYCKQRGINEEDAV
                     ALIVSGFCKEVLNELPMEFAVEARALLAVNLEGAVG"
     gene            2357..3100
                     /gene="sufC"
                     /locus_tag="QGH35_05850"
     CDS             2357..3100
                     /gene="sufC"
                     /locus_tag="QGH35_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007646876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HJL82088.1"
                     /translation="MLSIENLGVSIANKTIINGLNLKVNSQEIHAIMGPNGSGKSTFA
                     KTLAGHPECLISNGEITYLGTNINELEIDERANKGIFLGFQYPIEIPGLSNTIFLKTA
                     LNAQRKAQNKNAITAAEFLKELQDNARLMGIDQSFLHRGVNENFSGGEKKRNEILQML
                     MLKPKLSILDETDSGLDIDAMKTVANGVNHMRKNDSSIILITHYERLLKYIKPDFVHV
                     LSQGRIVESGDSTLASKLEEKGYEWLLKK"
     gene            3184..>3482
                     /locus_tag="QGH35_05855"
     CDS             3184..>3482
                     /locus_tag="QGH35_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82089.1"
                     /translation="MKTLIDLKEKAIPSKKDEKWQYTNIEPCIEKSLELFARKNDRKK
                     SKKSNNPLIEKNNDKDIIIYYHNGYFFSQQNLPNYIKTLSTSEEPETFKSLLDRK"
BASE COUNT         1258 a          592 c          628 g         1004 t
ORIGIN      
        1 taaagaattt aaatactctg ggaaagcaac acctgtatcg tttgattgtc tcagagtttc
       61 ttcaaataat attacaccac tgatatagtc tgaaatgcct gcagaagtga ataacatgtt
      121 gcgataaatt tgcctgttta tctctgttga ttctgtatca atatctttga atcttttacc
      181 aatggtcggt gtgctctcat cagccgcaag aataccttta ttgggtgcaa ccattgcttg
      241 ggcaatggaa tttaattcat ttgagttcat tcttatctct cctaatttat gttgttgatt
      301 ataacgaacc acctctgtca atttctatta catttttcaa aaaagtaaag aaattataca
      361 caaattcaat atattggact aaaatagtac taattaatat atgcacacaa cgttttatgt
      421 taagaattaa taaaatgaca gactatggca tgctgattct agggaatctt gcagtcagtc
      481 atggtggcta tttatctgca aaagatatag cctcctccac tcatatctct ctgcccactg
      541 tccagaaact tttaaaaaaa ctaaacaaaa ataatctggt cgtctccaaa caaggtccat
      601 tgggtggcta tgctttggat aaggcaacta aattgactag cgtttcaaaa atcttagaag
      661 ctcttgaagg agacctatca ctaatggaat gcagtgctaa acaaagtaca tgcgaaatag
      721 aaaattcctg tcaaattgga aacgcatggc aagttattaa tcaaactatt ttttcctctc
      781 tagataaatt aacccttctg gatctcatca aaccttcaac cattgagaaa tatattccaa
      841 cagagatagt gatccatcag aatccaaaaa gacaatagaa tatggagtcg aacaaacgaa
      901 agaagaacgc tcctgaactg gaagaattca ttgataaaaa atatcagtat ggctttgtca
      961 ctgatataga atcagaaacc ttaccacctg gtcttgatga aaacgtgatt cgcttgattt
     1021 cagagaaaaa aaatgaacca gaatttttac ttgaatggag attaaaagct tttaaatttt
     1081 ggcaaaaatt aaagccgcca atgtggggtc atttaaaaat tgatgacatt aactaccagg
     1141 ctatttcata tttttcaaag cctaaacaag agaatgcacc tcaatcacta gaagatgtgg
     1201 atccaaaatt actagacacc tatgaaaagt taggtatacc actccatgaa agagcagcac
     1261 ttgcaggcgt tgctgtcgat gctgtgtttg atagcgtttc agttactacc acttttaaag
     1321 aaaaacttgc tgaagcgggt gttatctttt gtcccttctc agaagcagta agacaatacc
     1381 cagacttaat taaaaaatat ctagggaaag ttgttcctta tagagataac ttctatgccg
     1441 cactaaatgc ggcagttttt accgatggtt catttgtcta tattcctaaa ggtgttcgct
     1501 gtcccatgga attatcgacc tattttagga tcaatgaggc taaaacaggt caattcgaaa
     1561 gaactttaat cattgccgat gaaggtagtt atgttagtta tctagaagga tgcacagcac
     1621 ctatgcgaga tgaaaaccaa cttcatgctg ccgttgttga gttaattgcc cacgatgatg
     1681 cagaaattaa atactccact gtacaaaatt ggtatccagg agatgagagt ggtcaaggag
     1741 gtatatataa ttttgtcaca aaacgtggag attgcagggg ccacaactca aaaatatcat
     1801 ggacacaaat tgagactggt tcagccatta catggaaata tcccagttgc attttaagag
     1861 gtgataaatc agttggcgag ttttattcca tagctcttac aaataatttc caacaggcag
     1921 ataccggaac aaaaatgata cacataggta aaaatactaa aagcaaagtg atcagcaaag
     1981 gtatatcagc tggtcatagc caaaatagtt ataggggctt agttgctgta tccaaaaatg
     2041 cagaagcttc gagaaattat acaaactgcg attcactttt aatcggtgct gattgtggtg
     2101 ctcatacatt tccttccatg gaaattcata actcatcaac catactggaa cacgaagcat
     2161 ctgcatccaa agtctctgat gagcaattat tttattgcaa gcaaagaggt ataaatgagg
     2221 aagacgccgt tgctttgatc gtaagtggat tctgtaaaga agtattaaat gagcttccta
     2281 tggaatttgc ggttgaagca agagcattgt tagctgttaa tttagaaggt gctgttggat
     2341 gagtaaatca atagaaatac tatcaattga gaatttgggc gtctccatag cgaataaaac
     2401 tataatcaat ggtctaaatc tgaaagtaaa ttctcaagaa attcatgcca tcatgggacc
     2461 caatggatct gggaaaagca cttttgcaaa aacgttagcc ggacaccctg aatgtctaat
     2521 tagtaatggg gaaattactt atttaggaac caatattaat gaactggaaa tagatgagag
     2581 agccaataaa ggaatttttt taggtttcca gtaccctatc gaaataccag gattatcaaa
     2641 tacaattttt ttaaaaactg cccttaatgc tcaaagaaaa gctcagaata agaatgcaat
     2701 aacagcagct gaatttttga aagagctaca agacaatgca cggttaatgg gtatagacca
     2761 gagtttttta cataggggtg ttaacgaaaa tttctcaggt ggagagaaga aacgaaatga
     2821 aatacttcaa atgttgatgc taaaaccaaa attatccatt ttggatgaaa cagactcagg
     2881 attagatata gatgccatga aaacggttgc aaatggtgtg aatcatatga gaaaaaatga
     2941 ttcatcaatt attttaatca ctcactatga acgattactc aaatatatca aacctgattt
     3001 tgtccacgtt ctaagtcaag gtcgtatagt tgaatctggc gattcaactc tagctagtaa
     3061 attagaggaa aaaggctacg aatggcttct aaaaaaataa attcctatct ccaagagtat
     3121 accgacgatc caattgatca tcttacaaag aacagtggga tcaaatggct agaagaatta
     3181 aaagtgaaaa cactcattga tttaaaagaa aaagccatac cttcaaagaa ggatgagaaa
     3241 tggcaataca ctaatattga accttgtatt gaaaaaagct tggaattatt tgcaagaaaa
     3301 aatgaccgca aaaaatcaaa gaaaagcaac aacccattga tagaaaaaaa taatgataaa
     3361 gatattatta tttactatca caatggatat ttcttttctc agcaaaactt acccaattac
     3421 ataaaaacat tatctacatc agaagagcct gaaactttta aatcattatt ggataggaaa
     3481 tt
//
LOCUS       DASCTH010000275         3844 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000616450, whole genome shotgun sequence.
ACCESSION   DASCTH010000275 DASCTH010000000
VERSION     DASCTH010000275.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000616450"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="QGH35_05860"
     CDS             complement(<1..472)
                     /locus_tag="QGH35_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HJL82090.1"
                     /translation="MKTIIHKISFKLNGEKIEQEVPSSLLLIDLIRDTLKLKGTKPGC
                     LEGECGACTVLIDGVGINSCLYLALNVDGKSVTTVEGLASNVEPLSDVQNALVEHGAV
                     QCGFCTSGIVMNIHSFKDKCDDDNMTPDREDIKKSLEGNICRCTGYVKIIDAVES"
     gene            complement(500..3016)
                     /locus_tag="QGH35_05865"
     CDS             complement(500..3016)
                     /locus_tag="QGH35_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HJL82091.1"
                     /translation="MKIDKIKARINAPADSAFILQGNAAFALGVIHAGYHAATGYAGT
                     PSTEVIDKTLAHAQDHMSVGWSVNESVAVSTGVGHAIAGNDALVTMKIPGVFQAADAI
                     TTSAFFSVDSGALVIYAATDYLPSSTQHVIDSRHFFASSRLPVLEPRNHQEMYDIPKI
                     AADMSKKYNTPVIILTSGILAHSEALIHTKSPRLVDPVDLPDTLHDWMLLPSIARQNY
                     NKATQERIPSIQKWAETSPLVSEIKGKDDWGIITCGNTEGIVLEALNSLSLNPSILSL
                     GITNPIPQGRIKQFTDNISGNIYVIEDGDRFLEEKIRLSRVNVTGKEEQSVLTLWTPD
                     DIVDFLSNHLDLPKPGNVIQTDLEPLPRPPSICPGCSYKAFALSVAKLKKQKKIYASF
                     GDIGCSTLLFFNNALDTVSCMGASDPMRQGFVLSRPDMAHKVISVIGDSTECHSGMDG
                     TRNAIFRNIPGVKVILDNYSTAMTGQQPAPSSKTNLAGQDITFDLHKAIEAEGGRTVV
                     VDAYDVKKVDKALKEALKLAEEKVYTTLILEGECIHNVEKSKLARDLEFDYDNCKRCD
                     LCNICPGIQTDDEKTPSFTSLCSNCASSSQVCLQRCPFDAINKKDDLGNYQKDFPLLD
                     FPKFTAPVALDKSKFPKSLKIAIRGIGGQGNLFFGKVLTEVALRTPYIDTNIIKGDTL
                     GMAQLGGSVISTFSCGDVYSSVLAPFSADALVVMEQSEVLRPGFLDLLKPGGTIILNE
                     FSALPINANKDDYPKIEEIQDALSNFNIIHINAHQIAIDMNDSSGISTNVIIIGLLSK
                     INPFSQIPSEIWQEAILELSPNDLIKTINLSGYNQGRTLA"
     gene            complement(3021..>3844)
                     /locus_tag="QGH35_05870"
     CDS             complement(3021..>3844)
                     /locus_tag="QGH35_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014564.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HJL82092.1"
                     /translation="GISAFGPDHPDRAGYDPALQAMFGYMHLTGDPDRDPMLCGLPII
                     DLKAGDEAFAQVLLAMMEKQETGEGKKIDISMAQCASSWLITALPQLEFVKDEKDLFT
                     RSGNEHRSFVPSNCYPTKDGYVYLAVGSDDQWKKLTEIDGFSHLSLGNRVTNNGRKID
                     KDNIYKDIRKGLESYTTQVFIEVCLENNLAVAPVHNVKDVAQLDFIDKLMVKTNLPNG
                     DEVKLFPAPMETEFLTQNNHKIKCAPKLGENNESILSEIGMTHSDISHLQNEGII"
BASE COUNT         1134 a          773 c          689 g         1248 t
ORIGIN      
        1 aggactcaac agcatcaatt attttaacat aacccgtgca acggcaaata ttgccttcaa
       61 ggcttttctt tatatcttct ctatccggag tcatattatc atcgtcacat ttatctttga
      121 aagaatgaat attcataact attcctgagg tgcaaaaacc acattgaaca gcaccatgtt
      181 ctaccaatgc attttgcaca tcacttaacg gctctacgtt actggctaaa ccttcaactg
      241 ttgttacaga ttttccatca acattaagag cgaggtaaag gcaagaatta attcccacgc
      301 catcaatcag aacggtacaa gcgccacatt caccttctaa gcagccaggt tttgtccctt
      361 ttaattttaa agtatcacga attaaatcaa tgaggagcaa agacgatgga acttcttgtt
      421 caattttttc cccatttaac ttgaaggata ttttatgtat aattgttttc atattagtgt
      481 tgatttaata ttatttacct taagccaatg ttctaccctg gttgtagcca gataggttaa
      541 ttgtttttat caaatcgttt ggtgataatt ctaaaatagc ttcttgccat atttcactag
      601 gaatttggct gaatggatta atctttgata ataatccgat aatgataaca ttggtagaaa
      661 tcccagaact atcattcata tctatagcaa tttgatgagc gttaatatgt attatattaa
      721 aattgcttaa agcatcctga atctcttcaa tttttggata gtcatcttta ttagcattga
      781 tgggtaatgc agaaaattca tttagaataa tggttccacc tggttttaat aggtctaaaa
      841 atccagggcg aagaacttca ctttgttcca taacaacaag cgcatcagct gaaaaaggtg
      901 ccagaacgga agaatacaca tcaccgcaac taaaagttga aatgacagat ccacccaatt
      961 gagccattcc caatgtatcc cctttaataa tgtttgtatc tatatagggt gttcgtagtg
     1021 ccacttcggt taatactttt ccaaaaaaca aatttccctg cccaccaatg cctcggattg
     1081 caattttcaa tgatttgggg aactttgatt tatccagtgc cactggtgcg gtaaattttg
     1141 gaaaatctaa aagaggaaaa tccttttgat aatttcctaa atcatctttt ttattaatgg
     1201 cgtcaaacgg acaccgttgt agacaaactt gcgagcttga tgcacaattt gaacataaac
     1261 tggtgaaact aggtgttttt tcatcatcgg tttgaatgcc tggacaaata ttacataaat
     1321 cacatcgttt gcaattatca taatcaaatt caagatctcg tgctaattta cttttttcaa
     1381 cattatgaat acattctcct tcaagtatga gcgttgtata tactttttct tccgctagtt
     1441 ttaatgcttc ctttagcgct ttatctactt tttttacgtc atatgcatca accacaactg
     1501 ttcgaccgcc ttccgcttca atggctttat gaaggtcaaa tgttatatct tgtcctgcta
     1561 agtttgtttt tgaacttggg gctggttgtt gtcccgtcat ggcggtggaa taattatcta
     1621 gaataacctt tacaccaggt atatttcgaa aaattgcatt ccgcgttcca tccattccgg
     1681 agtgacattc cgttgagtcg ccaataacac tgataacttt atgagccata tctggccggg
     1741 acaaaacaaa cccttgcctc attgggtctg acgcacccat gcatgaaacc gtatctaaag
     1801 cattattaaa gaataaaagg gtagaacatc caatatcgcc aaaagatgca taaatctttt
     1861 tttgtttttt taatttagca acggatagag caaatgcctt ataagaacat cccgggcaaa
     1921 ttgatggtgg tcgaggtaag ggttcaagat ccgtctgtat gacattgcct ggtttgggaa
     1981 gatctaaatg gttggataaa aaatcaacta tatcatctgg tgtccaaaga gttagaacgc
     2041 tttgttcttc cttgccggta acattaactc tagagagacg aatcttttcc tccaaaaatc
     2101 gatccccatc ttcaataaca taaatattgc ctgaaatatt atccgtaaat tgtttaattc
     2161 gaccttgggg aattggattt gtaattccaa gagataggat agaagggtta agacttaaac
     2221 tatttaatgc ttcaagcaca atcccttcag tattcccgca tgtaatgatt ccccaatcat
     2281 cttttccttt aatttcacta acgagaggcg atgtttctgc ccatttctgt atagaaggaa
     2341 ttcgctcctg agtggctttg ttataatttt gccttgcaat gctgggtagt agcatccaat
     2401 catggagcgt gtcgggtaaa tcgacagggt caactagtct gggagatttt gtgtggatca
     2461 atgcttcgga atgcgcgaga atccctgaag ttaaaataat aactggagta ttatactttt
     2521 tgctcatatc ggcagctatt ttaggaatat catacatttc ttgatgattc cttggttcaa
     2581 ggacaggaag cctggatgat gcaaaaaagt gccgtgaatc gattacatgt tgcgtactag
     2641 atggaagata atctgttgca gcataaatga ccaaagcgcc tgaatcaacg ctgaaaaaag
     2701 cagaagtggt aattgcgtcg gctgcttgga aaacaccagg aattttcatt gtaaccaagg
     2761 cgtcattccc cgcaatcgca tgcccaacgc cagtagatac agcgaccgac tcgtttacag
     2821 accaacctac cgacatgtga tcttgggcat gtgctaacgt tttatcaatg acttctgtac
     2881 taggtgtccc tgcgtagcct gtagctgcat gatatcctgc gtgaataaca cccagtgcaa
     2941 acgctgcatt cccttgtaaa ataaaagcac tgtccgctgg tgcattaatt ctcgctttaa
     3001 ttttatctat tttcattata ttaaatgata ccttcgtttt gtaaatggga tatgtcggag
     3061 tgcgtcattc cgatttcaga cagaatagat tcattattct ctccaagctt tggcgcacat
     3121 tttattttat gattattctg agttaaaaat tcagtttcca ttggcgcagg gaacaatttg
     3181 acttcatctc catttgggag attcgttttt accattaatt tgtcaataaa atctaattgg
     3241 gcaacatcct tcacattatg aaccggagcg acagctaaat tgttttcaag acagacttca
     3301 ataaaaacct gggtggtata tgattcaaga cctttcctta tatctttata aatgttgtct
     3361 ttgtctattt tccgcccgtt gtttgtcacg cgatttccta gtgatagatg agaaaatcca
     3421 tctatttccg taagcttttt ccattgatca tcacttccaa cagctaagta aacataaccg
     3481 tcttttgtag gataacagtt ggatggaaca aaactacgat gttcatttcc actgcgagta
     3541 aataaatctt tttcatcttt aacaaactct aattgtggta atgctgtaat tagccatgac
     3601 gatgcgcatt gtgccataga aatatcaatt tttttccctt cgccagtttc ctgtttttcc
     3661 atcatggcga gaagtacttg tgcaaatgct tcatctccag cttttaaatc aataatcggc
     3721 agaccacaaa gcataggatc tctgtcgggg tcaccggtga gatgcatata cccaaacatc
     3781 gcctgcaatg caggatcata cccagctcta tcaggatgat ctggaccaaa tgctgatata
     3841 ccac
//
LOCUS       DASCTH010000276         2317 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000619520, whole genome shotgun sequence.
ACCESSION   DASCTH010000276 DASCTH010000000
VERSION     DASCTH010000276.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000619520"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="QGH35_05875"
     CDS             <1..350
                     /locus_tag="QGH35_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019292.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HJL82093.1"
                     /translation="DWKVTAAFIGGLPAREVVIAALGTIYAIGDETTSESLLQNKLKH
                     ATWDDGKPVFTLPMTLGLLVFYAFCLQCFATIAIIRRETNSWRWPVVAWTYMTSLSYL
                     AALITHRLASLII"
     gene            complement(353..802)
                     /gene="tadA"
                     /locus_tag="QGH35_05880"
     CDS             complement(353..802)
                     /gene="tadA"
                     /locus_tag="QGH35_05880"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HJL82094.1"
                     /translation="MTHSDEYFMALAIDQASIAQTNGEVPVGAIFVDNNEVIAASGNA
                     PIALSDPTAHAEIMVIRKAAELKKNYRLGGSVFVTLEPCAMCVGAMIHARIQRLIFGA
                     SDDNVGAVLSIYNLAQSPYENHRIEVTKGVLEKKCQMLLKDFFRSKR"
     gene            complement(932..1477)
                     /locus_tag="QGH35_05885"
     CDS             complement(932..1477)
                     /locus_tag="QGH35_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HJL82095.1"
                     /translation="MFKCGLILFFGTHLIPSFSKIKNFVVMKVGLNGYLGIFVLFSVT
                     GMVLLMIGYKSGEELLYPVNNWFYLNARYFMLIACICLAAAFLETHTRKILRHPMLTG
                     IIIWTIVHLSVNPDRSSVMLFGSFLVFSVLSIITSEIRGKNLSGGTTHFKFDMIAIII
                     GVISFALLYRFHEGLFGVSLS"
     gene            complement(1593..2207)
                     /locus_tag="QGH35_05890"
     CDS             complement(1593..2207)
                     /locus_tag="QGH35_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HJL82096.1"
                     /translation="MKEFLTVAVLHFFAVASPGPDFILVSRQCLRYGRSVALWTSLGI
                     ALGILLHVALSIAGLSILIKQQPVILDWLKIIASLYIAYLGLHSILSRHSRNIGQESI
                     ESRTKEMKSITTGFLTNVLNPKAFIFFATVFALVIETSTTNLTKIALGVYMSLATFAW
                     FVFISILLTNETANTRFRKFIPWIEKVTGLLLLLIALQILLQEL"
BASE COUNT          770 a          466 c          394 g          687 t
ORIGIN      
        1 gggactggaa agttacagct gcatttattg gaggcttgcc agcaagagaa gttgtgatcg
       61 cagctctggg tacgatttat gctattggtg atgaaactac ttctgaatcc ttgctccaaa
      121 acaagctaaa acatgctacc tgggacgatg gaaaaccagt gtttacactg cctatgacac
      181 taggcctttt agtgttctat gcattctgcc tacagtgttt tgcaaccatt gccattatcc
      241 gtagggaaac gaactcatgg cgctggccgg ttgttgcatg gacttatatg accagtctaa
      301 gctacctggc tgctctgatt actcataggc tggccagttt gatcatttag gatcaacgtt
      361 ttgacctgaa aaaatctttt aataacattt ggcatttttt ttctaaaacg cccttggtaa
      421 cttcaatacg atgattttca tagggagatt gagctaaatt ataaatagac aaaacagctc
      481 caacattatc atcagaagca ccaaaaatta atctttgaat ccttgcatgg atcatcgcac
      541 ccacacacat cgcacacggt tccaaggtca caaaaactga accacccaat cgataatttt
      601 ttttcaactc agcagccttt cttatcacca ttatttctgc atgcgcagtg gggtcgctca
      661 aggctatggg tgcatttcca gatgcagcga tgacttcatt gttatcaaca aagatagctc
      721 caacaggaac ttcaccattg gtttgagcaa tacttgcttg gtcaatagca agcgccataa
      781 aatattcatc ggagtgagtc atcttaccgt ttccaattga ttaaataatt ctcccaattt
      841 tgttcgatca gatcgaaagt ctctggactc agtttttcaa gcattacttt tgaaatctca
      901 gaccaaatac taggaccaat cattggttgt tttatgataa ggagacacca aataaacctt
      961 catgaaacct atacaacaaa gcaaaagaaa tcactccaat tattattgcg atcatatcaa
     1021 acttgaaatg agtcgtgccg ccgctcaaat tttttcctct aatttcagaa gttattatgg
     1081 atagaactga gaaaactaga aatgaaccaa acaacatgac tgaagatctg tctggattaa
     1141 cgctcaaatg aacaatggtc cagataataa tacctgtaag catgggatgt cgcagaatct
     1201 tacgggtatg cgtttctagg aaggccgcag ctaaacaaat acaagctatc agcatgaaat
     1261 accttgcatt caaataaaac caattgttta cggggtacaa caattcttcc ccagatttat
     1321 agccaatcat cagcagcacc attccggtca cgctgaacag tacgaatatt cccaaataac
     1381 cgtttagtcc aactttcatc acaacgaaat tttttatttt gctaaaactg ggaattaaat
     1441 gagtaccaaa gaacaaaatt aaaccgcatt taaacatgat aatctcctga tttatttgaa
     1501 ctcctttccg acaataaatt catttatgat tgtaaataag aaaactacta ttatcgatat
     1561 ttcttttaat cataaatcat gcttttattt ctttatagct cttgcaaaag tatttgtagt
     1621 gcaatcagta ataacaataa tccagtaacc ttctctatcc atggaataaa tttacgaaat
     1681 cttgtatttg ccgtttcgtt tgtcaataag atactgataa acacaaacca tgcaaaggta
     1741 gccaaggaca tataaacacc caaagcaatc ttagttagat tagtcgtaga ggtttctata
     1801 accaaggcga atactgtggc aaaaaatata aatgcttttg gattaagcac gttggttaag
     1861 aagcctgtag ttatggattt catttcttta gttctgctct caatagattc ttgaccgata
     1921 tttcttgaat gcctagataa aattgaatgc aggcctagat aagcaatata gagggaagca
     1981 attattttta accaatccag aataacaggc tgttgtttaa tgaggatgct taaaccagct
     2041 atagaaagag caacatgtaa aagaatgcct aatgcaatgc ctaaactagt ccatagagca
     2101 acacttctgc cataccttaa acactgacgg ctgactaaga taaaatctgg tccaggactc
     2161 gcaactgcaa aaaaatgtaa aacggcaaca gttaagaatt ctttcattgc tttgatttta
     2221 tatattatcg aagcaaattg tcatagacct ttaatgttat ggctttaaag ttacctccaa
     2281 aggaaccatg acgtgcacaa aatcattacc tttaaac
//
LOCUS       DASCTH010000277         3598 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000619650, whole genome shotgun sequence.
ACCESSION   DASCTH010000277 DASCTH010000000
VERSION     DASCTH010000277.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000619650"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..69)
                     /locus_tag="QGH35_05895"
     rRNA            complement(<1..69)
                     /locus_tag="QGH35_05895"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(259..1473)
                     /gene="tyrS"
                     /locus_tag="QGH35_05900"
     CDS             complement(259..1473)
                     /gene="tyrS"
                     /locus_tag="QGH35_05900"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HJL82097.1"
                     /translation="MIAKNKQLQEIRRGTEEILVEEEFVERLRQKKPLKVKVGFDPTA
                     PDLHIGHTVIINKMRQFQEFGHEVIFLIGDFTGMIGDPSGVNETRPVLTKEQIEENAK
                     TYESQIFKILDPKKTRIEFNSTWMNKISSSQLIEITSKYNVARMLERDDFKQRFKSGN
                     SISIHEFLYPLIQGYDSVALEADVEIGGTDQKFNLLIGRHMQQLYGQKPQSVITMPLL
                     EGLDGVKKMSKSAENYIALTDSPADMFGKMMSLSDELMWRYFELLSARSLDAIKELKQ
                     QVQSGDNPRDIKFLIAGEMVERFHSKQSADQAKELFINQFKHGQLPDDIATVSVKISE
                     VESESGSINIPKVLYLSTLVNSVTEGHRAIEAGSVRLEGEKIENSQLKLDLKTKHIFS
                     IGKRRFAKVEFN"
     gene            1532..2662
                     /locus_tag="QGH35_05905"
     CDS             1532..2662
                     /locus_tag="QGH35_05905"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HJL82098.1"
                     /translation="MSKRVKSNYYIGVMSGTSIDSIDAVLMEIQSKSWSLLGTLSKKF
                     PEQLRKDLLEVSRNKQKIDLRELIELDIKTGVAFAKCINALIKKAKINYKEINAIGLH
                     GQTIMHHIHTKYPGSLQIGNPSTVAQITGIGVVCDFRNPDITAGGEGAPLAPVFHSWL
                     FGAKKKNRVVVNIGGIANISFLGKKKSLSGYDIGPGNILMDVWASKHNHGNYDNNGNW
                     AKGGKVNMGLLDSFKSDPFFKKKPPKSTGTDYFNLQWITDHCQQNTPDKLSPRDIQST
                     LLELTTATIIDAIEAWPKSELAFCGGGVKNKHMIAILRKKLQVSLHSTTDWGIDPEWV
                     EAAGFAYLAKQRIENKRSYLGQTTGSRSKVMLGAIYEPPLPT"
     gene            complement(2674..3558)
                     /locus_tag="QGH35_05910"
     CDS             complement(2674..3558)
                     /locus_tag="QGH35_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82099.1"
                     /translation="MTKTYQRLFKFFRISFILILLICLVMEFYCRKPDEFKVRALSLP
                     SETEMAVLLFHGARDHLNPEVSAIATKFTKLLITRSNTEVINYDWSFAANNRLRASAN
                     AIEVGKILGRELATMDQLKHLRIIAHSGGAFVPDELCRTYRNAGGLAYIEITFLDPFG
                     LRGFLDRGFGVRSHGSCADFALSIVNTDDPAPTTNSILKNAWNLDVTGLPRPTDFIYR
                     KDLRTVDTDFWLGVGETLDDVKRNLNNPEQQHNGHYWPPYYFLTILDEKMAQPAERQH
                     QQFPRGSVIDAPPVLIAE"
BASE COUNT         1137 a          705 c          647 g         1109 t
ORIGIN      
        1 ccgttcgact tgcatgtgtt aggcatgccg ccagcgttca atctgagcca ggatcaaact
       61 cttcatatta aataaagttt gatttgtgct cacaaaatta caaaaaaaaa tttgtaggtt
      121 ttgctcgcac ctgtaactac tgtaccgaca cgagcaccca cacaaattac ctaaataaat
      181 tgttaaagat cacaatcact caactacttg agcgacgtgc aattataata gcactttaga
      241 aatgatcaat ggttaaattt aattaaattc aacttttgca aagcgtcgtt ttccgattga
      301 gaatatatgc tttgttttga gatctaattt aagttggctg ttctcaattt tctcaccctc
      361 aagtcgaact gatccagcct caatagcgcg gtgtccctca gttacactat ttaccaatgt
      421 tgatagataa agaaccttag gaatattaat acttcctgac tcactctcta cctcagaaat
      481 tttgactgaa actgttgcaa tgtcatcagg caactgacca tgtttaaatt gattgataaa
      541 taattctttt gcctgatcag cgctttgttt actatgaaaa cgctcaacca tttccccagc
      601 aatgagaaac ttaatatctc ttgggttgtc tccagattga acttgttgct tcaattcttt
      661 aattgcatca agtgaacgtg cgcttaacaa ctcaaaatat cgccacatca attcatcaga
      721 taaagacatc attttaccaa acatgtctgc aggtgaatct gtcaatgcga tataattttc
      781 agcagattta gacattttct taaccccatc taaaccttcc aaaagaggca tagtgataac
      841 actttgtggt ttttgtccat agagttgttg catgtggcga ccaatcaaaa gattgaactt
      901 ttgatccgta cctcctatct ccacatcagc ttctaaagct actgagtcgt atccttgaat
      961 taaaggatac aaaaattcat gaattgaaat ggaatttccc gatttgaacc gctgtttgaa
     1021 atcgtcccgt tcaagcattc tagcaacatt atatttggat gtaatctcaa tcaattgaga
     1081 tgaactgatt ttgttcatcc aagtggagtt aaattcaatt ctggtttttt tcgggtcaag
     1141 tattttgaaa atttgactct cataggtttt ggcattttct tcaatctgtt ctttagtcag
     1201 aacgggtctg gtttcattca cgccagaagg gtctccaatc atacccgtaa aatcaccaat
     1261 caaaaaaatg acctcatgac caaattcttg gaattgacgc atcttattaa tgatgactgt
     1321 atggccaata tgtagatctg gtgctgtggg atcaaacccg accttaactt ttaggggctt
     1381 tttttgccta agccgttcaa caaattcctc ttcaacaaga atttcttccg tgcctcttct
     1441 aatctcttgg agttgtttat tcttagcaat catagtcaat gctttttatt atataggtca
     1501 ttcattatcg ggtatataat cagttaaata aatgtctaaa cgtgtgaaat ctaattacta
     1561 cattggtgtt atgtctggta ccagtattga ttctatagat gccgtactga tggaaattca
     1621 atcaaagtcg tggtctttgc ttgggactct gagcaaaaag tttcctgaac aattgagaaa
     1681 agatttgttg gaagtttcca ggaacaaaca aaaaatcgat ttaagagaat taatcgagct
     1741 cgatatcaaa accggagtag cattcgccaa atgtattaat gcgttaatca aaaaagcaaa
     1801 aatcaactac aaagaaatta atgctattgg attgcacggt caaactatca tgcatcatat
     1861 ccacactaaa tatcctggct cactgcaaat aggaaatccc agtacagttg cacaaataac
     1921 cggtatagga gttgtttgtg attttagaaa tcctgacata actgctgggg gtgagggagc
     1981 gcctctagca cctgtttttc actcttggct ttttggcgct aagaaaaaaa atagagttgt
     2041 ggttaatatt ggcggcattg caaatatatc ctttctgggc aaaaaaaaat ctttgagtgg
     2101 ttacgatatt ggaccgggta atattctaat ggatgtctgg gcttcaaaac ataaccatgg
     2161 aaactatgac aataatggca attgggctaa aggaggcaaa gttaatatgg ggttactaga
     2221 tagtttcaaa tctgacccct tttttaaaaa gaaaccacca aaaagtactg ggactgatta
     2281 ttttaatctg cagtggatta ctgatcactg tcagcaaaac acccctgata aactaagccc
     2341 aagagacatc caatcaacac tccttgagct cacaactgct acgatcatag atgcaataga
     2401 agcttggcct aaaagtgagc tagctttctg tggtggtgga gtaaaaaaca aacatatgat
     2461 agctatacta agaaaaaaac tacaagtatc acttcattca actactgatt ggggtataga
     2521 cccagaatgg gttgaagcag caggctttgc ttacctagca aaacaaagaa ttgaaaacaa
     2581 aagatcctac ttgggacaaa caactggttc tagaagtaaa gtcatgctgg gtgcaattta
     2641 cgaacctcca ttaccaacct aagaaactaa tagttactct gctataagca ctggtggagc
     2701 atcgataact gagccacgag gaaattgctg atgttgtcgc tcagccggtt gagccatttt
     2761 ttcatctagt attgttaaaa agtaatatgg aggccaataa tgaccattgt gttgttgttc
     2821 tgggttgtta agattgcgtt ttacatcatc caaagtttca ccaactccta accaaaaatc
     2881 tgtatctaca gtccgtaggt cttttctata aataaagtca gtgggtcgtg gcaagccagt
     2941 tacatccaga ttccaagcat tttttagaat actattggta gttggtgctg gatcatcagt
     3001 attaacaatc gaaagtgcaa agtcagcaca acttccatga gaacgtactc caaaacccct
     3061 atccagaaaa ccccttaaac caaaggggtc taaaaaagtt atctcaatat atgccaatcc
     3121 tcctgcgttt ctgtaggttc tgcataattc atctgggaca aaagcacctc cgctatgtgc
     3181 aatgatacga agatgtttca gttggtccat cgtagccaat tctcttccca agattttacc
     3241 aacttctata gcattagcgg atgcacgtaa tcgattatta gcagcaaagg accaatcata
     3301 atttatcact tctgtattac tccttgtaat tagaagctta gtaaattttg tagctatggc
     3361 actgacttca ggattgaggt gatcccgagc accatggaac aataatactg ccatctctgt
     3421 ctctgatggt aagcttagtg ctctaacctt gaattcatcg ggcttgcgac aataaaattc
     3481 cattactaaa catatgagca gaatcaatat aaagctgata cggaaaaact taaaaagcct
     3541 ttggtatgtt tttgtcatag ttgcctcgat ttaattttaa tggatgagtt taatcttt
//
LOCUS       DASCTH010000278         3072 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000624506, whole genome shotgun sequence.
ACCESSION   DASCTH010000278 DASCTH010000000
VERSION     DASCTH010000278.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000624506"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="QGH35_05915"
     CDS             <1..372
                     /locus_tag="QGH35_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015739.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HJL82100.1"
                     /translation="WNNNLILNDSAELYEVAKTVIPSNIPRLTGPEGSEKRVLDLFLI
                     INKELVGRGLYLETLILDRRGSWVFTIKPKIEIRLGKEAIDQRLERLYLMLDQDLLAK
                     MNQISYIDLRYSEGLAISWKK"
     gene            389..1621
                     /gene="ftsA"
                     /locus_tag="QGH35_05920"
     CDS             389..1621
                     /gene="ftsA"
                     /locus_tag="QGH35_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HJL82101.1"
                     /translation="MSRNNGNEIIAALDIGTSKIVAFVAEINEENELKIIGVGQQVSR
                     GLKKGVVVNIDATVDSINGAIRKAEEVANCEINTVFAGIAGSHVRSFDGHGIVKINEG
                     EVSINDINGALDASQAMNIPADQRILHVLPKGYIIDGHEGIREPISMSGVRLEADVHI
                     VTVARSAEQNIVKCINRCGLDVQQLVLEQLASSHSVLSRDEKDLGVCLVDIGGGTSDI
                     AIFIDGQIVATKVIPVGGNHVTNDIAYALKTPEKHAEEIKINHACTLSKLNNENELIE
                     VSSTGDRPSRQIESEQLATVVQARYKELFEVINDEIGKINTERLRAGIVLTGGASQLT
                     GASELAEEILGTDVRVGSPRAMSGTFENINNPLHATGTGLLWFALEKYENNPDDLVKQ
                     KALESPFAKIKSWFDSNL"
     gene            1763..2677
                     /gene="lpxC"
                     /locus_tag="QGH35_05925"
     CDS             1763..2677
                     /gene="lpxC"
                     /locus_tag="QGH35_05925"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HJL82102.1"
                     /translation="MLKQRTLSSIVKASGVGLHTGEKVQIALRPAAQNTGIVFRRVDL
                     DPVQQIQALAENVTDTMLGTSIGNNSASVMTVEHLLAAFAGLGIDNVLVDLHGPEVPI
                     MDGSSASFVFLIESAGIEEQNALKKFLRIKKTIRVEEGDKFAEFKPYNGYRISFEINF
                     DHPMIKKKTTNLSLDFSTLTFLKEISRARTFGFLKEVETLRSHNLALGGSLENAVVLD
                     EYRILNQDGLRYQEELVRHKILDVVGDLYLMGYIMVGEFNGYASGHELNNKLILKLFS
                     DQTAWEEIEESDTKNIPISYWTASLGKI"
     gene            complement(2682..2978)
                     /locus_tag="QGH35_05930"
     CDS             complement(2682..2978)
                     /locus_tag="QGH35_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82103.1"
                     /translation="MITSKLKHISEIIEDNYTNLAGLSRKASKQSSLSKYLKENLQSY
                     GDCITSAHINDSNVLIIGATSPELANRLRYEEAEIKALCGTIGQHPKKIKISSR"
BASE COUNT         1032 a          453 c          645 g          942 t
ORIGIN      
        1 tggaacaaca acttgatatt gaatgattca gctgaattgt atgaagtagc taaaaccgtt
       61 attcctagca atattccaag attgactggt cctgaaggct ctgagaagag agttttagat
      121 ttatttttaa tcattaataa agaacttgta gggagaggtt tatatttaga gacgttaatt
      181 ttggatcgaa gaggatcctg ggtttttaca atcaagccta aaattgagat aagattaggt
      241 aaagaagcaa tagaccaaag attagaaagg ttgtatttga tgttggatca agatctttta
      301 gcaaaaatga atcaaatttc atatattgat cttaggtatt ctgaaggatt agcaatttcg
      361 tggaaaaaat aaattaaatt ttcaatcaat gtctagaaat aatggaaatg aaataatagc
      421 cgctctggac ataggtacgt caaaaattgt agcatttgtt gctgaaataa atgaagaaaa
      481 tgagctaaaa attattggtg tagggcaaca ggtttccaga ggtttaaaaa aaggtgtagt
      541 tgtaaatata gacgctacag ttgattcaat taatggtgca atcagaaaag cagaggaggt
      601 tgctaactgt gaaataaata cagtctttgc aggcatcgct ggcagccatg taagaagttt
      661 cgatggacat ggcatagtca agattaatga aggagaagtc tctattaatg acataaacgg
      721 cgctcttgat gcttcacaag caatgaatat accagctgat caaagaatat tacacgtttt
      781 accaaaaggt tatattatcg acggtcacga aggtattcgt gaacctatta gtatgtcagg
      841 agttcgattg gaagccgatg ttcacattgt cacggttgcc agaagtgccg agcagaatat
      901 tgttaaatgc ataaataggt gcggattaga cgtccagcaa ctcgttctag agcagttggc
      961 ttccagtcat tctgttttat ctagagatga gaaagatttg ggtgtttgcc ttgttgatat
     1021 agggggcgga acatcagata tagcaatttt tattgatggg cagatagttg caacaaaagt
     1081 tatcccagtc ggaggcaatc atgtaacaaa cgatattgct tatgcactta agacacccga
     1141 aaaacatgca gaggaaataa aaattaatca cgcatgcact ttatccaagt taaacaatga
     1201 gaatgaactt attgaagtat ctagcacagg tgatcgtccc tcaaggcaaa tcgaatcaga
     1261 acaattagct accgttgttc aggctcgata taaagaatta tttgaagtca ttaatgatga
     1321 gatcggaaaa ataaatactg aaagacttcg ggccggaatt gttttaacag gaggagcttc
     1381 gcaactcact ggtgcttctg aattggcaga agaaatatta ggaacagatg ttagggtggg
     1441 atctccgaga gcaatgagtg ggacatttga gaatatcaat aatcctttgc atgctactgg
     1501 aactggattg ttgtggtttg cactggaaaa atatgaaaac aatccggatg atctcgtaaa
     1561 gcaaaaggct ttagaatctc cctttgcaaa gataaaatct tggtttgatt ctaatctttg
     1621 ataataaagg tagccattat taaataaata gctaaacaaa ctattgtctc aaaatatatt
     1681 ttatgataca tatgatctag cttatttata tgattttgaa aggttttctt attttattaa
     1741 ttaattggca agagtattgt aggtgttaaa acaaagaaca ttatcgagta ttgttaaagc
     1801 ttctggcgtt ggattgcata caggagagaa agtgcaaatt gcattgagac cggctgcaca
     1861 aaatactgga attgtcttta gaagagttga cctagatcct gttcagcaaa tccaagcatt
     1921 agcagagaat gttaccgaca ctatgcttgg aacttccatc ggtaataata gtgcctcggt
     1981 gatgactgtg gagcatttac ttgctgcttt tgctgggtta ggtatagata acgtattggt
     2041 tgatcttcat ggtccagaag ttccaattat ggatggtagt tcggcctctt ttgtcttctt
     2101 aattgaatcg gcaggaattg aagaacagaa tgccttaaaa aaatttttaa ggatcaagaa
     2161 aaccataaga gttgaagaag gtgataaatt tgcagaattt aaaccgtaca atggttatcg
     2221 aatttcattt gagattaatt ttgatcatcc gatgatcaag aaaaaaacca caaatctaag
     2281 tttagacttt tcaaccctta cgtttcttaa agagatcagc agagctcgaa cctttggttt
     2341 tttaaaggaa gtagaaacac tgcgttctca taacctagca ttagggggca gtttggaaaa
     2401 tgccgttgtt ttagatgaat atcgaatcct aaatcaagat ggtcttcgct accaggaaga
     2461 attagtgagg cataaaatct tggatgtggt gggggatctt tatttaatgg gttatataat
     2521 ggtaggagaa tttaatggtt atgcatcagg tcatgaatta aacaacaagt tgatattaaa
     2581 attattctct gatcagacgg cttgggaaga aatcgaggaa tctgatacaa agaacatccc
     2641 tatctcatac tggactgctt ctttaggaaa aatctaagca ctcatcttga actaatctta
     2701 atttttttcg gatgttgacc tatcgtcccg cataaggctt taatttctgc ttcctcatat
     2761 ctcagtctat tagccaattc aggcgatgta gctccaatga tcaatacatt ggaatcattg
     2821 atgtgtgcag aagtaataca gtctccgtat gactgaaggt tttctttcag atatttagac
     2881 agtgatgatt gcttacttgc ctttcttgaa aggccagcaa gatttgtata attgtcctca
     2941 ataatctcag aaatatgttt tagtttgcta gttatcaaaa tgttacagat tcaaattaga
     3001 gggcaataaa attttatcca tgaaggtata atagcagctg attgatatcg tgaaaattat
     3061 caacagtaaa at
//
LOCUS       DASCTH010000279         2129 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000626589, whole genome shotgun sequence.
ACCESSION   DASCTH010000279 DASCTH010000000
VERSION     DASCTH010000279.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000626589"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="QGH35_05935"
     CDS             complement(<1..583)
                     /locus_tag="QGH35_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012334.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HJL82104.1"
                     /translation="MKEFKGKTVVVTGAASGIGYALAERFAQENMNIVLADIEQDALD
                     AAVVKISDLGVEAVGIAVDVMDKNSVQSLAKQSIDAFGNIHILCNNAGVAPPAIDGAI
                     WEHDMSDWDWVMGVNFYGVLYGIQTFLPHMIEHQQEGHVLNTISMAGILGLEGSYGVS
                     KFAALSLSEGLFQSLKKINSKIGASALCPGFVAT"
     gene            complement(692..979)
                     /locus_tag="QGH35_05940"
     CDS             complement(692..979)
                     /locus_tag="QGH35_05940"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HJL82105.1"
                     /translation="MNDLTRKGTDFQKMVWAELKKIPFGEIRTYKEIAVAIGKPKAAR
                     AVANACGKNPYPIIIPCHRVVRSDGTIGGYTGDGGVEKKRQLLRQEDHLNF"
     gene            1037..>2129
                     /locus_tag="QGH35_05945"
     CDS             1037..>2129
                     /locus_tag="QGH35_05945"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HJL82106.1"
                     /translation="MNNSRRKFITHSVSALSLAATSNVINFPLYAKEISYGPERGIAR
                     LNWNENPYGPSKKALQAIIESSYEGAYYPDHLVTRLKNMIAEKHELTQNHITISAGST
                     QALTVLSQIQSRKNPIISSELTWDIPLLIARSMGAKIIRVENGHDLEINLQKMLSLHN
                     ENISMVHICNPNNPTGTLIDPEKLSQALRIISKTVLVLVDEAYNEVTDHPETNTMVKL
                     IKEGHNIAISRTFSKIYGMAGQRVGYIIAHPEIIRTINRNGTGEYSTAMAGVSGAIAC
                     YDDEEFLNYSKSKINEAKEMVIEVASSNGLETLPSQTNFIYVNLGNINADTFRDEMRK
                     QNIIIAGKYGKHTNWSRVSMGKIEDVQRYV"
BASE COUNT          675 a          469 c          374 g          611 t
ORIGIN      
        1 ttgtagctac aaaaccagga cacagggcgg atgctccaat cttactattg atctttttta
       61 aactttgaaa cagtccctcg ctgagtgaca aagcagcgaa cttgctcacc ccataactac
      121 cttcaaggcc caggatacct gccatcgaaa tggtattgag tacatggccc tcctgttgat
      181 gctcaatcat gtgtggcaga aaggtttgta tgccatagag gacaccataa aagttcactc
      241 ccatcaccca atcccaatca ctcatatcat gctcccagat cgctccatcg atagcgggag
      301 gtgcgacccc ggcattgtta cacagtatgt ggatattacc gaaagcatct attgattgct
      361 tggctagtga ttgaacggaa tttttatcca tgacatcaac tgctatccct actgcttcga
      421 cccccaaatc gctgatcttt acaacagcag catccagagc atcttgttca atatcagcca
      481 gaacaatatt catattctcc tgtgcaaagc gttcagctag tgcataacca atgccactgg
      541 ctgccccagt caccaccaca gttttaccct taaactcttt cattctatag acccctataa
      601 aaaaactaat tacttatgtt aatcaattag atttaattag tgaattttat cttaaccgaa
      661 acaagaatga tttctccagg atcatataca gtcaaaaatt caaatggtct tcttgtctta
      721 atagctgtct ttttttctca acacccccat cccctgtgta gccaccaata gttccatcag
      781 atcgaaccac tcggtgacat gggataatta ttggataagg gtttttacca catgcattgg
      841 caaccgcacg agcagcctta ggttttccta tggcaacagc tatttccttg taggttctga
      901 tttctccaaa aggtattttt ttcaattctg cccacaccat tttttgaaaa tctgttcctt
      961 ttctagtcag gtcattcatc acctccatat tttgcctatt agagaacaat tatgctattt
     1021 tgaatatatt caactgatga ataattctag aagaaaattt atcacacact cagtgtctgc
     1081 attgtcactg gctgcaacaa gcaatgtcat aaactttcct ttatatgcaa aagaaatatc
     1141 ttatgggcct gaacgtggca ttgcccgatt gaattggaat gaaaatcctt atggcccaag
     1201 taaaaaagca cttcaagcca tcatcgaatc ctcctatgaa ggtgcttatt accccgatca
     1261 tcttgttaca agattaaaga atatgattgc tgaaaaacat gaattgaccc aaaaccacat
     1321 aacgatcagt gctggatcaa cccaagcatt aacggttcta tcacaaattc aaagcagaaa
     1381 gaatccgata atcagttcag aattgacctg ggatatccct ttattgattg cacgatctat
     1441 gggtgcaaaa ataattcgtg ttgagaatgg tcacgattta gaaataaacc ttcagaaaat
     1501 gctgagtttg cacaatgaaa atatttctat ggtccatatt tgtaacccca ataaccccac
     1561 tggcaccctt attgatcctg agaaactctc tcaggctctg aggatcatat caaaaacagt
     1621 actcgttcta gttgacgaag cctacaacga agtgaccgat catcctgaaa caaataccat
     1681 ggttaaactg atcaaagagg ggcacaatat tgctatttca agaacatttt caaaaatcta
     1741 tgggatggca ggtcaaaggg tgggctatat tattgctcac cctgaaatta tcagaactat
     1801 taatagaaat ggcacaggtg aatattcaac tgcaatggct ggagtttctg gggctattgc
     1861 ctgttatgac gatgaggaat ttttaaatta ttcgaaatca aaaattaatg aagcaaaaga
     1921 gatggtcatt gaagttgcaa gtagcaatgg tttagaaaca cttccctctc aaacaaactt
     1981 tatctatgtc aatcttggca acattaatgc tgacactttt cgtgatgaaa tgagaaagca
     2041 aaacattatc attgccggca aatatggaaa acatacgaac tggtcaagag tcagcatggg
     2101 caagatcgag gatgtacaaa gatacgtcg
//
LOCUS       DASCTH010000280         2729 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000633707, whole genome shotgun sequence.
ACCESSION   DASCTH010000280 DASCTH010000000
VERSION     DASCTH010000280.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000633707"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="QGH35_05950"
     CDS             <1..207
                     /locus_tag="QGH35_05950"
                     /inference="COORDINATES: protein motif:HMM:NF016107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HJL82107.1"
                     /translation="FSRNPIYLGMTTILIGSALIYGSISTFFIPLVFMAAVNLIWIRY
                     EEKKLASIFGDDFKSYKERVRKWI"
     gene            257..475
                     /locus_tag="QGH35_05955"
     CDS             257..475
                     /locus_tag="QGH35_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82108.1"
                     /translation="MKQLFYIFLTIVLYSCDGRTPEEYDQDFKEQFNACIARTQSKCT
                     DQDENACQKKAVSRCEAFLGTIEKPKIK"
     gene            556..1005
                     /locus_tag="QGH35_05960"
     CDS             556..1005
                     /locus_tag="QGH35_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014953457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HJL82109.1"
                     /translation="MEDAKTISQSISWKCKHTWKRASVNTLWCLLGCSIGDFSTIYFF
                     QITEIPFPVTGIMVLAIINGLITSIMLETFILSRQMALILAFKTAIGMSLISMISMEA
                     AMNIVDVLLTGGATLVWWVLPFMLVAGFLTPLPYNYYRLKKFGKACH"
     gene            complement(1025..1744)
                     /locus_tag="QGH35_05965"
     CDS             complement(1025..1744)
                     /locus_tag="QGH35_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007225176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HJL82110.1"
                     /translation="MFPEVIENFFSNHQDLLYVITLCLDLGFTVMMFRFFGQNGLLAC
                     IVLSILLANLQGPKLTVIFGMQTTLGVIFYSSIFFATDLMSEKFGKQAANKAVMMGFF
                     ISVIILVMLSISLLFQPSIEGGQLFPTEVHQAFVRILDFTPRFIIGSLIAYLISQRFD
                     VWCFHKIKDWTNGKHLWLRNNLSTMASQTVDTTLFSLVVWWGVVDLTTAIELGLAKMV
                     FKIGIAAFDTPFIYWARSWKD"
     gene            1813..>2729
                     /locus_tag="QGH35_05970"
     CDS             1813..>2729
                     /locus_tag="QGH35_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82111.1"
                     /translation="MKSIFALLVLVIAGSASVFWYMNRIPPQLKEPNYYTYYKNQNMK
                     IEGRVGIFISHLIMPEDMAPNDFHNLAMKTKQYIPWPFKLLFDDDNGVLLLDEEKFYE
                     FEEFVPTKLIDARGNDKDIDGIPYIVKYEKGLMSWVPPSESLHLTHGYFLLHERNMGM
                     PTVSSKLINKANLYYYSGKGITGGKIPHASGNYEIASKTIEKIHQKYGPIPWRWVTAE
                     DFGLARAKMRSLLDEGVDTIILAPPRPTYSHHEEFNGSFKHSFEYIHEWEEENNKDIK
                     VILLPQLSEYPVIRQAYLQMLKDRLDNFP"
BASE COUNT          900 a          478 c          509 g          842 t
ORIGIN      
        1 ttttcaagaa acccgatcta tctgggtatg acaacgatct taatcggttc tgccttaatt
       61 tatgggtcca tttcaacttt ttttattccc ctggtattta tggcagcagt caatttgatt
      121 tggattcggt acgaagaaaa gaaattggca tcaatctttg gagacgattt caaaagctat
      181 aaggaacgtg tgagaaaatg gatttgactt gacctgtttt tcaaaaaacc tttaagaata
      241 tattaactgg ctaaccatga agcagctatt ttatattttt cttacaattg ttttatattc
      301 atgtgatggg agaactcccg aagaatatga tcaagatttc aaggaacaat tcaatgcttg
      361 tatagcaaga acccaatcaa aatgtacaga tcaagatgag aatgcgtgtc agaaaaaagc
      421 agtctctaga tgtgaggcat ttctaggtac aatagagaag ccaaaaatta aataagctat
      481 aaccatagtg gttaaaaata tttactgata catcctttaa tcagttatca ttaatacgtt
      541 aatatggaga tgatgatgga agatgctaag acaatatcgc aatcaatttc atggaagtgc
      601 aagcacactt ggaaaagggc gtctgtcaac actttgtggt gtctattagg atgttcaatt
      661 ggcgattttt caacaatata ctttttccaa attacagaaa taccatttcc tgttacaggg
      721 attatggtgc ttgcaatcat caatggatta attacctcaa ttatgctgga gacattcatt
      781 ttgtctcgtc agatggctct catccttgca tttaaaactg cgattggcat gtcattaata
      841 tcaatgatct ccatggaggc agcaatgaat atcgttgatg ttttattaac cggtggtgca
      901 accctggtat ggtgggtttt accgtttatg ctggtggctg gttttttaac ccctttgcca
      961 tacaattatt atcgtttaaa aaaatttggc aaagcctgtc attgattttt taactcaatc
     1021 tttctcaatc cttccagctt ctggcccagt aaataaaggg cgtatcaaat gctgcaatac
     1081 caatcttaaa caccattttc gctaaaccaa gctctattgc ggtggttaaa tccaccacgc
     1141 cccaccaaac aaccagcgaa aacaaagtcg tatctacggt ttgtgaagcc atggttgata
     1201 aattgtttct taaccaaaga tgtttgccgt tggtccaatc tttgattttg tgaaagcacc
     1261 atacatcaaa tcgttgacta atcagataag cgattaatga gccgatgata aagcgaggtg
     1321 taaaatctag aattctgacg aatgcttggt ggacttcagt aggaaatagt tgaccccctt
     1381 caattgaagg ctgaaacaat agtgaaatac tcaacatcac taaaatgatg acactgatga
     1441 aaaatcccat catcacagcc ttgttagctg cttgctttcc aaatttttca ctcatcaggt
     1501 ctgttgcaaa aaaaatactg gaataaaaaa taacgccaag agtggtttgc atgccaaaga
     1561 ttacagttaa ttttggaccc tgaagattgg ccagtaatat tgataagaca atacaagcaa
     1621 gtagaccatt ttgcccaaaa aagcgaaaca tcataaccgt gaatcccaaa tcaagacaca
     1681 aggttattac atacagaaga tcttgatggt tagagaaaaa attttcaatt acttcaggaa
     1741 acattagaat tggttgttaa ttatcatcta agagtaaatt atatttatta ttatcaggca
     1801 atgaaaaaag ttatgaaatc aatttttgca ctcctcgtct tagtcatagc aggtagtgct
     1861 tcagtttttt ggtatatgaa tcgaatcccc ccgcaactga aagagccaaa ttattacact
     1921 tattataaga accaaaatat gaaaattgaa ggcagggttg gaatctttat ttcacactta
     1981 attatgcctg aggatatggc tccaaatgat ttccataatt tggccatgaa aacgaaacaa
     2041 tacatacctt ggccttttaa attattgttt gacgatgata atggtgtttt attgttggat
     2101 gaagagaaat tttatgaatt tgaagaattc gttcctacca agctcattga tgctcgtggc
     2161 aatgataaag acattgatgg cataccttat attgttaagt atgaaaaagg gttaatgtct
     2221 tgggtgccgc caagtgaatc tctacattta actcacggtt acttcttgct tcatgagaga
     2281 aatatgggga tgcctacggt atccagtaag ttaatcaata aagccaattt gtactattat
     2341 tctggcaaag gtatcacagg tggaaaaata cctcacgcat caggtaatta tgaaattgca
     2401 tcaaagacca ttgagaaaat acaccaaaaa tatggcccca ttccatggcg gtgggtgact
     2461 gcagaggatt ttggattagc cagagccaag atgagatcat tactcgatga aggtgtggat
     2521 acgatcatcc ttgctcctcc aagacctact tattctcatc atgaagagtt caatggaagt
     2581 tttaaacatt catttgaata cattcatgag tgggaagaag aaaataataa ggatatcaaa
     2641 gtgattttgt tgccccaatt atcagaatat cctgtcatta gacaggctta tctacagatg
     2701 cttaaagacc gtctggataa ttttcctga
//
LOCUS       DASCTH010000281         1531 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000634517, whole genome shotgun sequence.
ACCESSION   DASCTH010000281 DASCTH010000000
VERSION     DASCTH010000281.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000634517"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(21..1433)
                     /locus_tag="QGH35_05975"
     CDS             complement(21..1433)
                     /locus_tag="QGH35_05975"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017509194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HJL82112.1"
                     /translation="MAISVFDLFKIGIGPSSSHTVGPMIAARKFILSIEKSNQLKKVT
                     RIKSEMYGSLGATGEAHGTPKAIILGLEGEQPEQVDVTLITKRIETIYSEGRINLLGK
                     QTIDYNHEIDLKFYRRKSLPFHPNGMIFSAFDENGKELVQKTYYSVGGGFVVDDSNEN
                     GNYIAEDETAITYPYDTATELLEYCKRKSLKISDVMIKNESAWRPENKTREELLNLWS
                     VMEETVNNGIANKGVLPGGLNVPRRAHKLHQQLTKKSDKKSDKKSDDPLTILDWVTLY
                     ALATNEENAAGGRVVTAPTNGAAGIIPAVLHYYVRFVPKADDEGIVRFLLTAAAIGIL
                     YKKNASISGAEVGCQGEVGSACSMAAGALAEVLGGTPTQVENAAEIAMEHNLGLTCDP
                     IGGLVQIPCIERNATASVKAIHAARMALQGDGTHFVSLDRVIRTMRKTGEDMHLRYKE
                     TARGGLATHVLEVPVNIIEC"
BASE COUNT          418 a          330 c          276 g          507 t
ORIGIN      
        1 accatactca taataaccta ttaacattca ataatattta caggaacttc tagcacatga
       61 gttgctaatc ccccacgagc tgtttcctta tatcgaagat gcatatcttc accagtcttt
      121 cgcattgtgc ggatcactct atctaacgaa acaaaatgag ttccatctcc ctgaagtgcc
      181 atacgggcag catggattgc cttaactgat gccgtggcat tacgctcaat gcaaggtatt
      241 tgtactaagc cgcctattgg gtcacaagta agccctaaat tatgttccat ggcaatttcg
      301 gctgcatttt caacttgtgt tggcgtgcca ccaagaactt ctgctaatgc tccagctgcc
      361 attgagcaag cagatccaac ttcaccttgg caacctactt cagcgccgct tatggatgca
      421 ttctttttgt aaaggatacc tattgcggcg gcagttaata aaaatctaac tattccttca
      481 tcatccgctt tgggtacaaa gcgcacataa taatgcagaa cagctgggat aatgccggct
      541 gctccattag taggggcagt aaccactctc cctcctgcgg cattctcttc gttggttgct
      601 aatgcataaa gggttaccca gtcaagtatt gtcaacggat catcagattt tttatctgat
      661 tttttatctg atttttttgt taattgttgg tggagcttat gggctcggcg tgggacattc
      721 aaaccccctg gcaatactcc cttattagca ataccattat taacggtttc ttccataaca
      781 gaccataagt ttaataattc ttcacgggtt ttattttctg gtcgccatgc gctttcattt
      841 tttatcatca catccgatat tttgagggat tttcttttgc agtattctaa aagctcagta
      901 gctgtatcat aagggtaagt gattgcggtt tcatcttcag cgatgtaatt tccgttttca
      961 ttagaatcat ctactacaaa accacctccc acggaatagt aggttttttg gactaattcc
     1021 tttccattct catcaaaggc agaaaatatc attccgttag gatgaaaagg gagggatttt
     1081 cttcgataaa acttaagatc tatctcatgg ttgtaatcaa tagtttgttt tccaagtaaa
     1141 tttattcgtc cttcagaata aattgtttcg attcgtttgg tgattaaagt tacatcaact
     1201 tgctcaggtt gctctccttc taaacctaag ataatggctt taggtgtacc gtgagcttcc
     1261 cccgtggcgc caagggaacc atacatctct gattttatac gagtcacttt ttttaattga
     1321 ttggattttt ctatactcag tataaatttt cgtgcagcga tcataggacc aacggtatgg
     1381 gagctggatg gtccaatacc aattttaaaa agatcgaaaa cgctaattgc cataattttt
     1441 cacaaatgct ctgtaatcat tcgctctaaa tatcttaaag ccatcaaatt gattcaataa
     1501 actcaagatc attaggcttt ataagatctg a
//
LOCUS       DASCTH010000282         5375 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000634686, whole genome shotgun sequence.
ACCESSION   DASCTH010000282 DASCTH010000000
VERSION     DASCTH010000282.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000634686"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            30..629
                     /locus_tag="QGH35_05980"
     CDS             30..629
                     /locus_tag="QGH35_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020028928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HJL82113.1"
                     /translation="MNPLDKISGTIIAGLVIAVIIAVGTSLSGASAANSIVIWLHVAA
                     GVAWIGLLYYFNFVQVPSIAAAKSDSEGPGPGAITKYVAPRALLWFRWAALVTWLSGA
                     LFLYLNGNLGDAFELGLRTGNTYATTIGIGAWLGTIMLFNVWILIWPNQKKVLGMVEA
                     SEEEKAKGARIAFLASRTNTMLSIPMLMSMVGTGHGFFM"
     gene            complement(676..2100)
                     /gene="yidC"
                     /locus_tag="QGH35_05985"
     CDS             complement(676..2100)
                     /gene="yidC"
                     /locus_tag="QGH35_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HJL82114.1"
                     /translation="MTTANTKNIVINTPYQQIEFNEKTALPIHWNICYPDCSDRAKIV
                     LNVANEANQFFSIEQLQSLEEVMYQSVFIENKDAMVLMFELRDDMDIEGMTTSISKIN
                     YVISKTSFQADMIVYSSNPIKIKIDGRDALQPENITGLGGLYNGNQLITVTPQGIEAI
                     EQNSWVKDSKEIWHGIRTRFWSFLISPRNNIQSYTIQDVDSHSFVLEALSDSNQYHFS
                     FYLGPVEKSALVSVSTQLKQLLYTALWNWLRWICFGLQMILGWVYSWLGHMGWSIIIL
                     SVIVKILLYPLSRLAEKWQHEVHEIQAWLQPRLKEIKAQYTGEEAHKRTLALYREKQV
                     NPMFTFKSLAGLLIQIPVFIAVFDMLGESILLKQQSFLWIKDLSMPDHWMNLPFLIPF
                     FGESFNLLPLLMMLVTALGAFFFQDQHLQGTLLKQQKRKLYLMAFAFFILFYTFPAGM
                     VLYWTTSNSLQLMKVIFLRCRTST"
     gene            complement(2145..3950)
                     /locus_tag="QGH35_05990"
     CDS             complement(2145..3950)
                     /locus_tag="QGH35_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HJL82115.1"
                     /translation="MITSRKILLRWLSWYGFVNGLISAVIGIRYLFFYSFPTELSVIS
                     YVPLALITHFISLNYLSMVLLMMPMAILIPNKRFIFCLSIILMASINTLLILDTNFFA
                     ENRYHLSLLTSVLFDQRTYMLLAFQFLIVLIFEFVLAQQLFIRINNPDKRQLNGKPLV
                     YVLLVSWIYVQGLHIWADATYNNAVTGFTRYLPLYRPLHAKRDLARLGLIDSERLRNE
                     RSIEKLGDFYSEQLNYPINPLSCQQRDREVKNIIVLLIDALRPEMVSNRITPAIDEWR
                     TKALQFENHFSGGTSSRMGTFSLLYGIPTTYWRTYHDNQRASILIDQLTSYNYDVVAI
                     SSGGFGSPTLLDRTAFSQIKNLNVKKLSPSVKASNHMVTDKWMSWLSNYNRETPFFAY
                     LHYDPPMNSIENFDADLIPNQFLKVQEESHLQEVGRYTESIHLVDQQVGAIHDSLIET
                     GLIHNTVIIITSDHGYELNDYGTGYYGHSSNYSPAQMKVPFFLSWPGKESSKISKMTS
                     HNDLVPTLLSEILGCINPTSDYSSGFNLFGDQSWKYLVAGSYDSYAIVSEKKTVVSYG
                     GYFEVLDNNYNLIRSDDLDKSLIESAMKEMKRFYK"
     gene            complement(3956..5086)
                     /locus_tag="QGH35_05995"
     CDS             complement(3956..5086)
                     /locus_tag="QGH35_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016357.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HJL82116.1"
                     /translation="MSKLLTAWQEYQKYKKLTPAGKRIVIFSESYQDWHHFEPLVTGL
                     TTEFNQEICYVSSDPSDPGLATEHPLIHAFYIPEGIIQIIFFQFLDAELMILTMMDLN
                     NYELKRSIHPIHYVYLFHSLASTHMVDHANSYDHYDTLLCTGPHQVREIRAREKQFGL
                     KPKNLIPYGYHRLDALMNEKVNNQIGQISKVLLAPTWGDNSILNLVGLDLCQTILDGG
                     HFLTVRPHYETVKRTPDIIDEIKHRFQDHSNFNLVLGMGENRSLLDSNVLITDWSGIS
                     FEYSMGLEKPVIFIDVPPRIRNPDWQELQMEPLEVSIRDKVGRIVALDEIYSISETIN
                     QMLSEKDQYSLNINKLRDEWVYNLSQTRSVGAKEIVKLLKQS"
     gene            complement(5101..5319)
                     /locus_tag="QGH35_06000"
     CDS             complement(5101..5319)
                     /locus_tag="QGH35_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82117.1"
                     /translation="MMKQTTTTIFFLVFIATTGFSQPVYAYLDPSTGSMLISAVIGMF
                     ATVGLVLKTYWYKVKSFFKKTPEEIDKE"
BASE COUNT         1704 a          991 c          992 g         1688 t
ORIGIN      
        1 atcactcatt aatagttaga ggtaatatta tgaatccatt agataagatt tcagggacaa
       61 ttattgcagg tttagtcatt gcagtcataa ttgcagttgg caccagtttg agcggtgcaa
      121 gtgctgccaa ttcaatagtg atctggttgc atgtagcagc tggtgtcgcc tggattggtt
      181 tactttatta ttttaatttt gtccaagttc cttcaattgc agcagcaaaa tctgattctg
      241 aaggaccggg tcctggagca atcactaaat atgttgctcc aagagcactg ctatggttcc
      301 gatgggccgc tttggtaact tggttatccg gtgctttatt cttatactta aatggaaatc
      361 ttggagatgc atttgaactt ggcttgagaa caggtaatac atacgcaaca accattggga
      421 ttggtgcttg gcttggaaca attatgttgt ttaatgtctg gatcctaatc tggccaaatc
      481 aaaagaaggt cctcggtatg gttgaagctt cggaagaaga aaaagccaag ggggccagaa
      541 ttgcatttct tgcatctaga accaatacga tgttatccat tccgatgctc atgagtatgg
      601 ttggtactgg acacggtttc ttcatgtaaa ggattgaggt atcagtctaa attataaagc
      661 ccggcttgcc gggctttatg ttgatgttcg acatcttaag aaaatgactt tcattagttg
      721 caaactgttt gaggtcgtcc agtacaaaac catccctgct gggaatgtat aaaagagtat
      781 gaagaatgca aaagccatta aatataattt ccgtttctgt tgttttagca aagtcccctg
      841 taagtgttga tcttgaaaaa agaaagcacc taaagctgtt accaacatca taagcaatgg
      901 caataaattg aatgactcac caaagaaagg aattaaaaag ggtaaattca tccaatgatc
      961 cggcatcgat aaatccttga tccatagaaa actttgttgc ttcagtaaaa tactttcacc
     1021 taacatatca aaaacggcaa taaatacagg aatctggatc aataaaccag ctagactttt
     1081 gaaagtaaac atcgggttca cttgtttttc tctatacagt gccaacgttc ttttatgagc
     1141 ctcctcacca gtatattgtg cttttatctc tttcaatctt ggctgaagcc aggcttgaat
     1201 ctcatggacc tcatgttgcc atttctcggc tagtctggat aaaggataga gcaatatctt
     1261 tacaatcaca gacaatatga taatagacca acccatatga ccgagccaag aataaaccca
     1321 acccagaatc atctggagac caaaacaaat ccaccgaagc caattccata aggcggtgta
     1381 gagaagttgt tttaattgag tagaaacact cactagtgct gatttttcta cgggacctaa
     1441 ataaaaactg aaatgatact gattagaatc tgataatgcc tctagtacaa agctatgact
     1501 atcaacatcc tgtatggtgt aagattgaat attatttctt ggagaaatta agaaagacca
     1561 aaatcgggtt ctgattccat gccagatttc cttagaatcc tttacccaag agttttgctc
     1621 tattgcctca ataccttgag gtgtgacagt tatgagttga ttgccattat agagtccacc
     1681 caaaccggtg atattctctg gttgaagagc atctctgcca tcgattttta ttttaatagg
     1741 attagaagaa tatacaatca tatctgcttg aaaggatgtt ttggaaatga cgtaattgat
     1801 cttactgatt gaggttgtca taccttcaat atccatgtca tcccgcaatt caaacataag
     1861 taccattgca tctttgtttt cgatgaaaac agactgatac ataacctctt ctaaggattg
     1921 gagttgctct atagagaaaa attgattggc ttcattggca acatttaaaa ctatttttgc
     1981 cctatcactg caatctggat agcaaatgtt ccaatgtatt ggtaaagcag ttttctcgtt
     2041 gaattctatt tgctgatatg gagtattaat gactatgttt ttggtgttag ccgtagtcat
     2101 aaataccatt agaaaaagaa ataagattac aaatggtacg gatctcattt ataaaatcgc
     2161 ttcatttctt tcattgctga ttcaattaga ctcttatcca aatcatcaga gcggatgaga
     2221 ttataattgt tatccaaaac ttcaaaataa ccgccatagg aaacaacagt ttttttctca
     2281 gatacaatag cgtatgaatc ataactacct gcgaccaaat acttccaaga ttggtcacca
     2341 aatagattaa agccactgct gtaatcactt gttggattga tacacccaag aatttcggat
     2401 agtagagtag gaaccaagtc attgtgagac gtcatttttg aaatcttgct gctttccttg
     2461 cccggccatg aaagaaagaa tgggactttc atttgtgcag gactgtaatt gctagaatga
     2521 ccataataac cggttccata atcatttaat tcataaccat ggtcagaggt aatgattatt
     2581 acggtattat gtattaatcc tgtttcaatc agagaatcat gaattgcccc gacttgttga
     2641 tcaactaggt gtatcgattc agtatagcga cccacttcct gtaaatgact ttcctcctgg
     2701 actttaagaa attgattagg aatcaaatcc gcatcaaaat tctctattga attcataggg
     2761 gggtcatagt gaagataagc aaaaaaagga gtttcccgat tataattgga caaccaggac
     2821 atccatttat ccgtaaccat gtggttactt gcttttactg aagggcttaa ctttttcacg
     2881 ttcaagtttt ttatctggga gaatgcggtt ctgtcaagca aagttggact cccaaatcca
     2941 cccgaactaa tcgcaacaac atcatagtta taacttgtca attgatcgat taaaattgag
     3001 gcacgttgat tgtcatgata agtcctccaa taggttgttg ggatgccata gagtaatgaa
     3061 aatgtcccca tcctggatga agttccaccg ctgaaatgat tttcgaattg aagggctttt
     3121 gttcgccatt catcaatagc aggcgtaatt ctgttactca ccatttctgg tcgtagcgca
     3181 tcaattaaaa gaacaattat attttttact tctctgtctc tttgctgaca tgataatggg
     3241 ttgatagggt aatttaattg ttctgaatag aaatcaccta gtttttcaat agaacgttca
     3301 tttcttaatc gttctgaatc aatcagacct aatctcgcta aatcgcgttt tgcatgtaat
     3361 ggtcgataca aaggtagata acgggtaaac cccgtcaccg cattgttata ggttgcatct
     3421 gcccaaatat gaaggccttg tacataaatc cagctgacca gcaatacata aactaatggc
     3481 ttaccattta actgtctctt gtctgggttg ttgatcctaa taaataattg ctgtgcaaga
     3541 acgaattcaa aaatcagaac aattagaaat tgaaaagcaa gcaacatata agttcgttgg
     3601 tcaaacaata cactggttaa tagagacaag tgatatctgt tctcagcaaa aaaattagtg
     3661 tcaagaatca gtagagtatt gatgctagcc atcagaatga tagatagaca aaaaatgaat
     3721 cgtttgtttg gaattagtat tgccatgggc atcattagca ggaccattga taaataattc
     3781 aaactgataa aatgagtaat caacgccagt ggtacataac tgatgactga gagttcagtg
     3841 gggaaagaat agaagaatag atatcgaatg ccgatgactg cagaaatcag tccgttgaca
     3901 aatccatacc aactcaacca acgtaaaagt atttttctgc tggtaatcat gaaacctagg
     3961 actgctttag taattttacg atttcttttg cccccaccga tcttgtttgg cttaaattat
     4021 aaacccactc atctctcaat ttattgatat ttaatgagta ttgatccttc tctgataaca
     4081 tttgattaat ggtttcagat atagaataaa tttcatccag agcaacaatt ctaccgacct
     4141 tatcgcgaat acttacttcc agtggttcca tttgtaattc ttgccaatct gggtttctaa
     4201 tcctaggagg cacatcaata aagatcactg gtttctccaa acccattgag tattcaaacg
     4261 aaattccaga ccaatcagta atgagaacat tcgaatccaa aagagatcga ttttcaccca
     4321 tgcctagaac cagattaaaa ttggaatgat cttgaaaacg atgtttaatt tcatcaatga
     4381 tgtcaggtgt tcttttaaca gtttcatagt gtggtctgac tgtcagaaaa tgacccccgt
     4441 ccaatatagt ttggcaaaga tccaatccga ctaaattgag tatactgtta tcgccccaag
     4501 ttggagctaa tagtaccttg gaaatctgtc cgatttgatt attaactttt tcattcatta
     4561 aagcatccaa acgatgataa ccatagggaa tcagattttt tggcttcaat ccaaactgct
     4621 tctcccgagc tcttatttct ctcacttgat gaggtccagt acaaagcaaa gtatcgtaat
     4681 gatcataaga attggcatga tcaaccatat gtgtactggc taagctatga aaaagatata
     4741 cataatggat aggatggatc gatcttttta attcatagtt gtttaaatcc atcatggtaa
     4801 gaatcatcag ttctgcgtct agaaactgaa aaaatataat ttgaatgatt ccttctggaa
     4861 tataaaaggc atgaattaga ggatgctctg tcgccaatcc cggatcgcta ggatcggatg
     4921 aaacatagca aatttcttga ttaaattccg tcgttagtcc agttacgagt ggctcaaagt
     4981 gatgccaatc ctgataactt tctgagaaaa ttacaattct ttttccagca ggtgttagtt
     5041 ttttatactt ttggtactcc tgccatgctg tcaacaattt agacactatc aattagtttt
     5101 ttattcttta tcgatttctt caggagtttt tttgaagaat gatttaactt tataccaata
     5161 ggtttttagc accaatccta ctgtggcgaa cattccaata actgcagaaa taagcatgct
     5221 tccagtgctt ggatctaaat aggcataaac aggctgacta aatcctgtcg ttgcaataaa
     5281 gaccagaaag aaaatagttg ttgtagtctg tttcatcata atcactcggt aataatgtta
     5341 tgttattttt aaaccaaaat ggagttatta gtaac
//
LOCUS       DASCTH010000283         5389 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000634888, whole genome shotgun sequence.
ACCESSION   DASCTH010000283 DASCTH010000000
VERSION     DASCTH010000283.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000634888"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="QGH35_06005"
     CDS             complement(<1..271)
                     /locus_tag="QGH35_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013856.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HJL82118.1"
                     /translation="MISKTNKHHWRTRQHEDFYFKLSKQQGFRTRSIYKLIEINEKYR
                     LIKPKSWVLDLGCSPGSWSDYIVNELSNKGRVISVDVSVMKPIANV"
     gene            complement(284..760)
                     /gene="greA"
                     /locus_tag="QGH35_06010"
     CDS             complement(284..760)
                     /gene="greA"
                     /locus_tag="QGH35_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HJL82119.1"
                     /translation="MGKVFMTQLGNDSLREELKMLISKDRPEVINAIASAREFGDLKE
                     NAEYHAAKEKQSFIEGRISEIESKLSNAEIIDVTKLDIREKVVFGSTVKLRNMDSKSI
                     VKYKIVGEDEADIDNGLISYMAPISRAIIGKSLLDIVEFESSEEKTIFKIQSIEYI"
     gene            complement(761..3982)
                     /gene="carB"
                     /locus_tag="QGH35_06015"
     CDS             complement(761..3982)
                     /gene="carB"
                     /locus_tag="QGH35_06015"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HJL82120.1"
                     /translation="MPKRKDISSVLIIGAGPIVIGQACEFDYSGTQACKALKEEGYRV
                     ILVNSNPATIMTDPDIADSIYIEPINWQTIEKIIEREKPDAILATMGGQTGLNTALDL
                     STNGILEKHNVEMIGATKKSIDTAEDRELFKIAMKEINIEVAKSGLVHNLDQAFEVIN
                     KINYPVIIRPSFTLGGTGGGIAYNEDEFITIVKRGLELSPTNEILLEESLLGWKEFEM
                     EVVRDNQDNAIIVCSIENVDPMGIHTGDSITVAPAQTLTDKEFQRMRDQSIAILRKIG
                     VDTGGSNVQFAINPSDGRMIVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGFTLDE
                     LKNDITDRQTPASFEPTIDYVVTKIPRFAFKKFPQADPNLTTQMKSVGEVMAIGRTFQ
                     ESLQKAIRSLETNHYGLTNLYKDKSVTNNLRKKLITPSAERLFYIGDAFRLGLSISEV
                     NHMTHIDPWFLEQIKDLIDTEINISRLNIENIDSETLLTLKKKGFSDIRLAELLHSDE
                     LTIRAKRKVLKIKPVFKRVDTCAAEFETSTAYLYSTYEDECESRPTNKRKILILGGGP
                     NRIGQGIEFDYCCVHASFAISECGYESIMINCNPETVSTDYDTSDRLYFDPLTFEDVM
                     EIIEIEQPEGVIVQFGGQTPLKLAKRLLDAGAPIIGTLPDSIDTAEDREQFKDLVTTL
                     GYLQPYNEVASNLDEALQKAKKVRYPMVVRPSYVLGGRAMEIIYNDEELKKYMHEAVR
                     VSNEAPVLLDHFLDKAIEVDVDAVSDGKEVFIGGIMEHIEQAGVHSGDSSCVIPPIKL
                     SQKIQNTLKDQMINLAKSLNVVGLVNAQFAVRKNKVFIIEVNPRASRTVPFVSKATGN
                     QLAKIAAKTMIGIPLNDQEIKTSTPMKFFSVKSPVFPFNKFPDTDPILGPEMKSTGEV
                     MGTGRTFGEAYMKAQNATGITIPQSGSVFISVRDPDKTVNLLRLAEFLIRKEFEIIAT
                     DGTNRFLNDNNIQSSHINKVHEGKPHIVGLIRKELIDLIINTTEGKQSIEESLSIRAE
                     AVIRNVTYYTSLEAAVATMNSFDYFNIMNVTKIQDYYKVEVHR"
     gene            complement(3961..5139)
                     /gene="carA"
                     /locus_tag="QGH35_06020"
     CDS             complement(3961..5139)
                     /gene="carA"
                     /locus_tag="QGH35_06020"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011469206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HJL82121.1"
                     /translation="MIPSFFCKTGKIKEYKLKPAILALEDGTIFRGKSIGADGHTTGE
                     VVFNTSMTGYQEILTDPSYYKQLVTLTYPHIGNTGINQADFESTKAFVAGLIIRDISS
                     GNNWRKEQSLDNFLKEQNIIAISEIDTRKLTNHIRLKGSLRGCIVNDDLNERNAIKKS
                     KEFPGLSGLDLAKAVTTESTYEWADGSLDIFNETVAIKDLNYHVVAYDFGIKTNILRS
                     LVDYGCKLTVVPAMTTYESILSLNPDGIFLSNGPGDPEPCDYAIDAIEKLLKENIPIF
                     GICLGHQLLGLASGAKTVKMKFGHHGANHPVQNIKTGRVFITSQNHGFMLDEKRLPET
                     LCVTHRSLFDGSIQGIERNDCKAFSFQGHPEASPGPHDVTELFLKFIKMMDICQNEKI
                     "
BASE COUNT         1552 a         1040 c          886 g         1911 t
ORIGIN      
        1 caacgttagc tattggtttc attacactaa catctacaga gataactctc cctttattag
       61 ataattcatt gacaatgtag tcagaccaac taccaggtga gcaacccaaa tccaataccc
      121 aagactttgg cttgattaac ctatattttt catttatttc aattaactta tagatcgatc
      181 ttgtacgaaa accctgttgt ttagacagtt taaaataaaa gtcttcatgt tgacgagttc
      241 gccaatgatg tttgttggtt ttgctaatca atttagaaaa ttactaaata tattcaatgc
      301 tctgaatctt gaatattgtc ttttcttctg aagattcaaa ctcaacaata tcaagcaaac
      361 tcttacctat tattgctctt gaaataggag ccatatatga aatcagtcca ttatctatat
      421 ccgcttcatc ttcaccgaca attttatact ttactatgga ttttgaatcc atatttctta
      481 gttttactgt tgaaccaaaa actacctttt ctctgatatc tagttttgtt acatcaatta
      541 tttcagcatt agaaagtttt gactcgattt ctgatattct gccttcaata aaactttgct
      601 tctcttttgc tgcatggtat tcagcatttt ccttcaagtc accaaattct ctagctgaag
      661 caatggcatt aataacttca ggtctgtctt tagagatgag cattttgagc tcttctctta
      721 gagagtcatt acctaactga gtcataaaga cttttcccat ttaacgatga acctcaactt
      781 tatagtaatc ttgtattttt gttacattca ttatattaaa ataatcgaac gaattcattg
      841 ttgcaacagc agcttccaag ctagtgtaat aagtcacatt tctaattaca gcctctgccc
      901 taattgaaag ggattcttct attgattgtt tgccctctgt tgtatttatt ataaggtcta
      961 ttaattcttt tcttatcaaa ccaacaatgt gtggtttacc ttcgtgcact ttattaatat
     1021 gagatgattg aatgttgtta tcatttaaaa acctatttgt cccatctgtg gcaatgattt
     1081 caaattcttt tctaataaga aattctgcta gtcttaataa atttacggtc ttgtctggat
     1141 ccctgacaga tatgaataca gaacctgatt gagggatagt tatgcccgtt gcattctgtg
     1201 ccttcatata tgcttcacca aaagttctac cagtacccat tacctcgcct gttgatttca
     1261 tttctggacc tagaattgga tctgtatctg gaaatttatt aaatgggaaa acgggtgact
     1321 taacagaaaa aaacttcatg ggagtgcttg ttttaatttc ctgatcattt aatggaatac
     1381 caatcatagt ttttgctgct atcttggcca attgattacc cgttgccttg gaaacaaatg
     1441 gcacagtcct agatgctctt gggtttactt caattatgaa aactttattc tttctgactg
     1501 caaactgagc attaacaagt ccgacaacat ttaatgattt tgctaaatta atcatttgat
     1561 cttttaaagt attttgaatt ttttgtgaca actttattgg tggtattaca caggatgagt
     1621 caccagaatg aactcctgct tgctcaatat gctccatgat accgccaata aaaacttcct
     1681 tgccatcgct gactgcatca acgtcgactt caatggcttt atctaagaaa tgatccaata
     1741 gaactggtgc ttcattggaa accctgactg cttcatgcat atattttttt agctcttcat
     1801 cgttatagat aatttccatt gcccgaccac ctagtacata agagggtcta acaaccatgg
     1861 gatatctaac tttctttgct ttttgcaaag cctcgtcaag gttgcttgca acttcattgt
     1921 acggctggag ataaccgagt gttgtcacca agtctttgaa ttgctctcta tcttcagcag
     1981 tatcaataga atctggtaaa gtaccaatta tgggtgcccc agcatctaat aatcgtttag
     2041 ctaatttaag tggtgtttga cctccaaact gaacaatgac tccttctggt tgctcaattt
     2101 caataatttc cataacatct tcaaatgtta aaggatcgaa ataaagtcta tccgaggtat
     2161 cataatcggt tgaaacagtt tctggattac agttaatcat gatcgactca tagccgcatt
     2221 cagaaatagc aaaagatgca tgtacacaac aatagtcaaa ttcaattcct tgcccaattc
     2281 tgttaggacc gccaccgaga attaagatct ttcttttatt tgttgggcgt gattcacatt
     2341 catcttcata agttgagtat aaataggctg ttgacgtttc aaattctgct gcacaggtat
     2401 ctactctttt aaataccggt ttaattttca aaacctttct tttagccctg attgttaatt
     2461 catcactgtg caatagctca gccaatctga tatctgagaa acccttcttt tttaaagtaa
     2521 gtagtgtttc tgaatcaata ttttctatat ttaatctact gatgttgatt tcagtatcaa
     2581 ttaggtcttt aatttgttct aaaaaccaag gatcaatgtg agtcatgtga ttaacttcac
     2641 tgatactgag tccaagtcta aaagcatcac caatatagaa caatcgttct gctgaaggtg
     2701 taataagttt ttttcttaaa ttgttagtca ctgatttatc tttatacaga tttgttaatc
     2761 cataatgatt agtttctaaa cttcgaatgg ccttctgcag ggactcctga aaggttctcc
     2821 ctattgccat aacttcacca actgatttca tttgagtcgt taagtttgga tccgcttgag
     2881 gaaacttttt aaaggcaaaa cgtggaattt ttgtcacgac ataatcaata gtaggttcaa
     2941 atgatgctgg ggtctgacga tctgtgatat cgttctttaa ttcatcaaga gtgaatccaa
     3001 ttgctagttt tgcagcaatc ttagcaattg ggaaacctgt agccttagat gcaagtgctg
     3061 atgaccttga aacccttgga ttcatttcaa tgacaatcat tcttccatca ctaggattga
     3121 ttgcaaattg gacgtttgat ccacctgtat ctacaccgat ttttcgtaaa attgcaatgg
     3181 actgatccct cattctttga aattccttat cggttaaggt ctgagcaggt gcaacagtta
     3241 ttgaatcccc agtatggatc cccatgggat caacgttttc tatggaacag acaataatgg
     3301 cattatcttg attatctctg acaacctcca tttcgaattc cttccatcca agaagtgact
     3361 cttctaataa aatctcattc gttggagata actccaagcc tcttttcacg atcgttataa
     3421 attcatcttc gttgtatgca ataccaccac ctgtgccgcc taatgtaaaa gatggtcgaa
     3481 tgattacagg gtaatttatt ttattgatga cctcgaaagc ttgatctaaa ttgtgtacta
     3541 accctgactt tgcaacctcg atattgattt ctttcatagc aattttaaaa agctccctgt
     3601 cttctgctgt atcgattgat tttttggtag ctcctatcat ttcgacatta tgtttttcca
     3661 atattccatt ggttgacaaa tctaaagctg tattaagacc tgtttgtccg cccatggttg
     3721 caagaatggc atcaggtttc tctctttcaa ttattttttc tatggtttgc cagttaattg
     3781 gctcaatata aatagaatca gcaatatcgg gatctgtcat tattgtcgct ggattggagt
     3841 tgacaagaat aactcggtag ccttcctctt ttagtgcttt gcatgcttgt gtacctgagt
     3901 aatcaaattc acaagcttgg ccaataacaa ttggaccagc gcctatgatt aatacactac
     3961 ttatatcttt tcgttttggc atatatccat catttttata aatttcaaaa ataattctgt
     4021 gacatcatga ggtcctggac ttgcttctgg atgtccctga aaactgaatg ctttgcaatc
     4081 attgcgttct atcccttgaa tggatccatc aaacaatgag cgatgagtaa cacagagggt
     4141 ctctggtagt cttttctcat ctaacataaa tccatgattt tggcttgtaa tgaagactcg
     4201 tccagtttta atattttgaa cagggtgatt ggctccatga tgtccaaatt tcatttttac
     4261 tgttttcgca ccactggcca gtccaagtag ctgatgacct aggcagattc caaatatggg
     4321 gatgttttct ttcagcagtt tctcaatcgc atcaatagca tagtcacatg gctctggatc
     4381 acctggtcca ttggataaaa aaattccatc tggatttaat gaaagaatac tctcataagt
     4441 tgtcattgct ggcacgactg ttaacttaca gccgtaatca actaatgatc gtaaaatgtt
     4501 tgttttgatt ccaaaatcat atgccacaac atggtagttg agatctttta tagctacagt
     4561 ttcattaaaa atatcgaggc taccatcagc ccactcgtaa gtagattccg tagttactgc
     4621 tttggccaaa tctaaacctg aaagaccagg aaattctttt gatttcttta ttgcatttct
     4681 ttcattgaga tcatcattaa caatgcatcc acgtaaagac cctttcaatc ttatgtgatt
     4741 agttagtttt cttgtgtcga tttctgaaat tgcaataatg ttttgctctt ttaaaaagtt
     4801 atccaagctc tgttcttttc gccaattatt tccacttgaa atatccctaa taattaaacc
     4861 tgcaacaaaa gctttggtgc tttcaaaatc agcttgattg attccagtat tgccaatatg
     4921 aggatatgtg agggtaacta attgcttata gtaggagggg tctgtcagaa tttcttggta
     4981 accggtcatt gacgtattga atacaacttc accggtagtg tgaccatcag caccaattga
     5041 cttaccacga aatattgttc cgtcttcaag tgccaatata gctggtttca atttatattc
     5101 cttaattttg cctgttttgc aaaaaaagga aggaatcatt taaaggatcc ttccctcata
     5161 ttttcgtttg gaacccgtaa catcttattg tcttgacgaa ggtgtgtcac tatttaattt
     5221 tgaatttttt tatatttttt tctataggga atgtttaata agtaaaaaaa tccaaaagca
     5281 attagtatcg caattggaat atgaaatggg ggcgatggca tgaaattcat cagcgaatct
     5341 gcattgggct tactatttag ataccaataa ttagcagacg gtcctagta
//
LOCUS       DASCTH010000284         3050 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000639751, whole genome shotgun sequence.
ACCESSION   DASCTH010000284 DASCTH010000000
VERSION     DASCTH010000284.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000639751"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="QGH35_06025"
     CDS             <1..504
                     /locus_tag="QGH35_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013761.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HJL82122.1"
                     /translation="CLKLNQKYPKTIGVALQAKLFRTQDDMIELIDNNISVRLVKGAY
                     IEKKSIALNRYKDIHDNYLKLIELLRNKTNRRCAVATHDEIILNQILSKPDADRFDYE
                     FIYGVRRDLQFALRNQGQKVRVYLPFGENWLPYTLRRLREFKNFTFLVRNIYKEWFYG
                     IKGANRN"
     gene            507..1142
                     /gene="plsY"
                     /locus_tag="QGH35_06030"
     CDS             507..1142
                     /gene="plsY"
                     /locus_tag="QGH35_06030"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HJL82123.1"
                     /translation="MYELIVTACFGYLLGSLNGSLILGKINNFDIRHQGSGNAGATNI
                     FRMQGKALGILVLCFDSLKGFVAIWLVDTFIVSHVADPLFVSELYLYCLGLSVFLGHC
                     YPLWFQFRGGKGAATGLGVFIYFEPILVILSLMFWLLSLIVFRFVGLSTMIAFISFPL
                     FLYLSPNNDISLNLQIFSIILAVLVLFTHQKNIRSMIKGTEPKIDFFSKKK"
     gene            1139..2110
                     /locus_tag="QGH35_06035"
     CDS             1139..2110
                     /locus_tag="QGH35_06035"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HJL82124.1"
                     /translation="MKNELSIIRKIADGTPHNKNDLLNLIPSESQLLRIIRNIKEMGL
                     SIKEENDCYRLETPLELLSGSIIYSHLEQFDNRKNIAISVDDILESTSNKFHPYDIDD
                     NSVKVSITEYQLSGRGRENRQWFSPFGSGICFSIFQEIPNIKSPIGLSVFLGVKILGC
                     LEELGLKGIKVKWPNDFYFNNKKLGGLLIELSQNSADNLVATVGLGMNYLLPSNLQWD
                     DILSHSAIDIHSIKGDMTISRNFLAANLINTMIKSLDAFNDESLKDLQLVWGNFDMLI
                     GKQLKIQVGDDIYQGIDSGIDSLGQLILDDNGISKKVISGHILEWNN"
     gene            2190..2265
                     /locus_tag="QGH35_06040"
     tRNA            2190..2265
                     /locus_tag="QGH35_06040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2223..2225,aa:Thr,seq:tgt)
     gene            2342..2426
                     /locus_tag="QGH35_06045"
     tRNA            2342..2426
                     /locus_tag="QGH35_06045"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2376..2378,aa:Tyr,seq:gta)
     gene            2560..2633
                     /locus_tag="QGH35_06050"
     tRNA            2560..2633
                     /locus_tag="QGH35_06050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2592..2594,aa:Gly,seq:tcc)
     gene            2655..2727
                     /locus_tag="QGH35_06055"
     tRNA            2655..2727
                     /locus_tag="QGH35_06055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2688..2690,aa:Thr,seq:ggt)
     gene            2800..>3050
                     /locus_tag="QGH35_06060"
     CDS             2800..>3050
                     /locus_tag="QGH35_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HJL82125.1"
                     /translation="MAKEKFERNKPHINVGTIGHVDHGKTTLTAALTKIMADKFGGDV
                     SAFDDIDNAPEERERGITIATAHVEYESDARHYAHVDCPG"
BASE COUNT          979 a          486 c          619 g          966 t
ORIGIN      
        1 tgtttgaaat tgaaccaaaa atatcctaaa actataggtg ttgctctcca ggcaaaactc
       61 ttcagaaccc aagatgacat gattgagctc atagacaaca atatttcagt taggctggtt
      121 aaaggggctt acatagagaa gaaatctatt gcacttaatc gttataagga catacacgac
      181 aattacttaa aattgattga actgcttcga aataaaacga atcgaagatg tgctgtggca
      241 actcatgatg aaattatttt gaatcaaata ttgtccaaac ctgatgccga ccgttttgac
      301 tacgagttta tctatggagt cagacgcgat cttcaatttg cactcagaaa tcaaggtcaa
      361 aaggtgagag tctatctacc attcggagag aattggttgc cctatacgct tagaagatta
      421 agggagttta agaattttac atttttagtg agaaacatct ataaagaatg gttctatggc
      481 atcaaggggg ctaatcgaaa ttaagtatgt atgaattgat agtaactgct tgttttggat
      541 acctacttgg atctttaaac ggtagtttga tccttggaaa aataaataat ttcgacatta
      601 ggcaccaagg cagtgggaat gcaggagcca ccaatatttt tagaatgcaa gggaaggctt
      661 tgggcatatt agtcctttgt tttgacagct taaaaggatt tgttgcaatt tggttagttg
      721 atacatttat tgtttcgcat gttgccgatc ctttattcgt atctgaacta tatctatatt
      781 gcttaggact gtcagtgttt ttaggtcatt gttatccttt atggttccaa tttaggggag
      841 gtaaaggagc agctactggc ctgggggtct ttatttattt tgagccaatc ttagttattc
      901 tatctttgat gttttggttg ttgagtttaa ttgtatttcg ttttgttgga ttatctacaa
      961 tgattgcatt catctctttt ccattattct tatatttatc acccaataat gacattagct
     1021 taaatttaca aatattttca atcattttgg cagttctagt tctttttaca caccaaaaaa
     1081 atattagatc aatgatcaaa ggaacagagc caaaaataga tttttttagt aaaaaaaaat
     1141 gaaaaatgaa ctcagtatca tcagaaaaat tgctgatggt actccacata acaagaatga
     1201 tcttctaaat ttgatcccct ctgaaagtca attattgagg atcataagaa atatcaaaga
     1261 aatggggtta tcgattaaag aagaaaatga ttgttatcgc ttagagaccc ccttggaatt
     1321 actcagtggt tcaataattt atagtcattt agagcaattt gataatcgaa aaaatattgc
     1381 aatatctgtc gatgacattc tagaatccac cagcaataaa tttcatccgt atgatatcga
     1441 tgataacagc gtaaaggtta gtatcacaga gtaccaatta tcgggtcgag gcagggaaaa
     1501 tagacaatgg ttttcacctt ttggctctgg aatatgtttc tcaatttttc aagaaatacc
     1561 taacattaaa tcacctattg gattaagtgt tttcttggga gttaagatct taggttgttt
     1621 ggaagagctt ggtcttaaag gtatcaaagt gaaatggccc aatgatttct acttcaacaa
     1681 taaaaaatta ggtggtttgc ttatagaact gagccagaat agtgccgata acttagttgc
     1741 aacagttgga ctaggaatga actatctgct tccatccaac ctccaatggg atgacatact
     1801 gtcgcattca gcgattgata ttcactcgat caaaggtgat atgacgatta gtcgaaattt
     1861 tttggctgcc aatctaatta atacaatgat taaaagccta gacgctttca atgatgaatc
     1921 tctcaaagat ctacaacttg tttggggtaa ttttgacatg ctgatcggta aacaattaaa
     1981 gatccaggta ggtgatgata tttaccaagg tattgatagc ggaatcgata gtctgggaca
     2041 gcttatattg gatgacaacg gtatatcaaa aaaagtaatc tcaggacata ttctagagtg
     2101 gaacaattaa ataaacttcg gtctgttaat ctgatttaaa atgtttataa ttgccctcaa
     2161 aataaggtct atataattat taatagtgag ccggaatagc tcaattggta gagcaaccgc
     2221 cttgtaagcg gtaggttggg ggttcaattc cctcttccgg caccataaat taataataaa
     2281 tacctaaatt ttctcattga cacttgtata ggttaatcag aaaatagtgg gttatgtccg
     2341 cggaggggta cccaagcggt caacgggggc agactgtaaa tctgctggct tagccttcgt
     2401 aggttcgaat cctaccccct ccaccatttg taatggtgaa ttacatggtg gaaaaaggct
     2461 aaagaagtgc aatataaatt gtagaataaa tgaattagta gtaatgagta atgaaaaaaa
     2521 atagtgaaaa attatcgggt tggaacacat cgtttacgtg cgggcgtagt tcaatggtag
     2581 aacctcagcc ttccaagctg atgatgtggg ttcgatcccc atcgcccgct ccattctatc
     2641 gtgtgtgtgc atttgcccac atagctcagg tggtagagca cttccttggt aaggaagagg
     2701 tcaccggttc aactccggtt gtgggctcag gaagttttca ttatttatca tatcaattaa
     2761 aataattaat ttagtaattt agtatggaga aaaaaagtta tggctaaaga aaaatttgaa
     2821 cgaaataaac cgcatatcaa tgtaggaact attggtcatg ttgatcatgg taaaactact
     2881 ttgactgctg cattgactaa aattatggcc gataaatttg gtggagatgt ttctgcattt
     2941 gatgacatag acaatgctcc ggaagaacgt gagagaggca ttactatagc cactgcgcat
     3001 gttgaatatg agtctgatgc tagacattat gctcatgttg attgtcctgg
//
LOCUS       DASCTH010000285         3680 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000646888, whole genome shotgun sequence.
ACCESSION   DASCTH010000285 DASCTH010000000
VERSION     DASCTH010000285.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000646888"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="QGH35_06065"
     CDS             <1..1461
                     /locus_tag="QGH35_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HJL82126.1"
                     /translation="DSGWLLGSEENEVLSFKGIPFAASTEGINRWRPPQKKELWRGTR
                     DAQDYGDFCPQSKRDTLWFELGKTSEDCLSLNVWTPKKTSATKLPVMVWIHGGGYLQG
                     SGNIPRLNNPEFVKQDVVLVTINYRLNVFGFFAHPDLVQQSGDEMVGNYGLMDVIAAL
                     EWIQGNINQFNGDPNNVTVFGESAGASLISYLMISPQSKDLFHKAISQSSAVGLAPDT
                     RIDKQVGFKISAVDIAKKFTKRMGFESSQGIDQLRELSSSQIIDQLDPSTVFGPMIDG
                     ILIPNHVALLLAQGKQHDVPLIIGGNNWEASLGRQIGGGFSPKFISRLVPKKDKDVLY
                     PGLQGEQLEDRVFGDLVILSSARYMANQMKNIDSKTYHYHFSYVASEKTGKQPGAAHS
                     DDIAFVMKTLGVELASVTEKDEEISNLMHNYWVQFARTGDPNVPGQTNWTPYNQDNGS
                     VLEIGDTIILKKNFLNERINYHINRGINFLQSMEKR"
     gene            1458..2864
                     /locus_tag="QGH35_06070"
     CDS             1458..2864
                     /locus_tag="QGH35_06070"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HJL82127.1"
                     /translation="MKKTLESQNRKSLLEGTPDLGIRFDRLNRMLDMMISYQDQIPDA
                     LSKDFGHRSPMASKFTDIASIFDSIHFAKKHLKKWMKPEQRSAGSPFNLLGAKAYIQY
                     QPLGTVGVISPWNFPFNLTFAPLAGIFSAGNRAMIKPSEITPESSNLMKKMVSEFFDA
                     DELAVFCGDVSVAEDFSKLAFDHLLFTGSTSVARHVMRSAAENLVPVTLELGGKSPVI
                     IGSSANMKLVADRVWNGKLLNGGQICLAPDYLFVHENHVESWIQASTQSITEMYPSFF
                     DNDDYTSIINEHHFKRLQNYLDDAKQKGARLIEFNPAKEDAGNRTHFKMLPTVILGVN
                     DDMLVMQEEIFGPIIPVKTYSTIEDTVDYINGHERPLGLYYFGKDKTEETFILDNTIS
                     GGVTLNDVVFHVSQEDLPFGGIGPSGMGHYHGLEGFRRFSHAKGIYKQSPVDAVVKLT
                     RPPFTNMFDRLISSRIKK"
     gene            2871..3230
                     /locus_tag="QGH35_06075"
     CDS             2871..3230
                     /locus_tag="QGH35_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018300344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HJL82128.1"
                     /translation="MTQDRVLQDKVYDLGLDIVERHIFLCADQTEPKCAPKKKTLASW
                     DFLKRRLTELNLDKRGGIYRSKVNCFRVCMQGPIAVVYPDRIWYKQCTPEVLEKIIQQ
                     HLIQGTPVDEFVISKRN"
     gene            complement(3211..>3680)
                     /locus_tag="QGH35_06080"
     CDS             complement(3211..>3680)
                     /locus_tag="QGH35_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024834.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HJL82129.1"
                     /translation="QYITVLAGYGGVFALEAGLPPKYSGGPINGRIITFSLDADLSLP
                     NRPPNKKMPYPPESDADQITIANGEVKYHAHCFICHGAGAIGGGVISDLRYMNEDTHS
                     KFNAIVLGGLYTDKGMVSFAESFTQEEAEAVHAYLIQRAKETYYLETLNSFLK"
BASE COUNT         1133 a          660 c          791 g         1096 t
ORIGIN      
        1 gattctggat ggctactggg cagtgaagaa aacgaagtct tatcctttaa aggcatacct
       61 tttgccgctt cgaccgaagg gattaataga tggcgccctc cacaaaaaaa agaattgtgg
      121 agaggaacca gggatgcgca agattacggt gatttttgtc cacaaagtaa aagagacaca
      181 ttgtggtttg aattaggtaa aaccagtgaa gattgcttat cactgaatgt ttggacacct
      241 aaaaaaactt ccgctactaa gcttccagtt atggtatgga tacatggtgg tggttacctt
      301 cagggatctg gcaacattcc cagattaaat aatccagagt ttgttaaaca agatgttgta
      361 ttagtgacta ttaattatcg tttaaatgtt tttgggttct ttgcacatcc tgatttggtt
      421 cagcaatcag gtgatgaaat ggtaggtaat tatggattaa tggatgttat tgctgcactt
      481 gaatggatac aagggaatat caatcagttc aatggagatc ccaacaatgt cacagttttt
      541 ggagaatcgg caggagccag tttgattagt tatttaatga tttcccctca atctaaagat
      601 ctttttcata aagcgatcag tcaaagttca gcggttgggt tggcgcccga tacccgtatt
      661 gataaacagg taggctttaa aatatctgca gtagacattg ccaaaaaatt taccaaaaga
      721 atgggttttg aatcaagcca gggaatagac caattacgtg aattatcatc cagtcaaatc
      781 attgatcaat tagatccatc gactgtcttt gggcctatga tcgatggaat tttgattcct
      841 aatcatgtgg ctttgttatt ggcccagggt aaacagcacg atgttccatt aataatcggc
      901 ggcaataatt gggaggcaag tcttggacgg cagataggcg gtggctttag tccaaaattt
      961 atttcaagat tagtgcctaa gaaagataaa gatgtactct atccagggtt acaaggagaa
     1021 caacttgaag atcgagtttt cggagatctg gttattctaa gttcagctcg gtacatggct
     1081 aatcagatga aaaatataga ttcaaaaaca taccactatc atttttctta cgtggcatca
     1141 gagaaaacag gcaagcaacc aggagccgca cattctgacg acatagcatt cgttatgaaa
     1201 accttgggtg ttgaactggc tagtgttact gagaaagatg aagagatcag taatttgatg
     1261 cataattatt gggttcaatt tgctagaaca ggtgatccaa atgtacccgg ccaaacgaat
     1321 tggactccct ataaccaaga caatggtagt gtcctggaaa ttggtgatac tattattttg
     1381 aaaaaaaatt ttttgaatga gcgaattaat taccacataa ataggggcat taatttttta
     1441 caatccatgg aaaaacgatg aaaaaaactc tcgaatcaca aaacagaaaa tctcttttag
     1501 aaggcacacc tgatcttggt attcggtttg atcgattaaa ccgaatgctt gatatgatga
     1561 tttcttatca agaccagata cctgatgcac tcagcaaaga ttttggccac cgatcaccca
     1621 tggcctctaa gtttacggat atagcatcga tatttgattc gattcacttt gccaagaagc
     1681 atctaaaaaa atggatgaaa cctgaacagc gatcagcggg tagtcctttt aatcttcttg
     1741 gtgctaaagc ctacattcaa taccaacctt tgggtactgt tggtgtaatc agtccttgga
     1801 atttcccttt taatttaaca tttgctccat tggcaggaat tttctctgca ggtaatcggg
     1861 caatgataaa gccttctgaa attacacctg agtcctctaa tttgatgaag aaaatggtct
     1921 cagaattttt tgatgctgat gagctggctg tgttttgtgg cgatgtttca gtagctgagg
     1981 atttttccaa gttagctttt gatcacttac tatttactgg atcgacttca gttgctagac
     2041 atgtcatgag atcggcagca gagaatttag taccagttac tttggagtta ggtggtaaat
     2101 caccagtcat tataggctcc tcagctaaca tgaaactggt tgctgatagg gtttggaatg
     2161 gaaaattatt gaacggtggc caaatttgtc tagcaccgga ttatttattt gttcatgaga
     2221 accatgttga gtcttggata caagcttcta ctcaatcaat taccgaaatg tatccttcat
     2281 tctttgataa tgatgattac acctcaatca ttaatgaaca ccattttaaa cggttgcaga
     2341 attatctaga tgatgcaaag caaaaagggg caaggctaat tgaatttaat ccagcaaaag
     2401 aagatgcagg aaaccgtacc cattttaaaa tgttgccaac agtaattctg ggagtaaacg
     2461 acgacatgtt agtcatgcaa gaggagatat ttggtcccat tatacccgtc aaaacttatt
     2521 caaccattga agataccgtg gattatatca atggtcatga aagaccattg ggcctgtatt
     2581 actttggaaa agataaaaca gaggaaacat tcatactgga taatacaatt tctggtgggg
     2641 ttactttgaa tgatgttgtt ttccatgttt ctcaagaaga ccttccattt ggaggcattg
     2701 gcccaagtgg catgggtcat taccatggat tggaggggtt tagaagattt agccatgcta
     2761 aaggcatata caaacaaagt cctgttgatg ctgtagtcaa gcttactaga ccccctttca
     2821 caaatatgtt tgatcgctta atctcaagtc gtatcaaaaa ataaacagca atgactcagg
     2881 atcgagtgct acaggataaa gtatatgatt tgggtctaga tatagtagaa cggcatattt
     2941 tcttgtgtgc cgatcagaca gaaccaaagt gcgccccgaa gaagaaaaca ctagcgtctt
     3001 gggacttttt aaagcgtcgc ttgaccgagc ttaatttgga taaaagggga gggatttatc
     3061 gttccaaagt caattgcttt agagtttgca tgcaagggcc aattgcagta gtttacccgg
     3121 atcgtatttg gtataaacaa tgcactcctg aagtgttgga aaaaattatt cagcagcacc
     3181 ttatccaagg aactcctgtg gacgaatttg ttatttcaaa aaggaattaa gagtttctag
     3241 gtagtaggtt tcttttgctc tttgaatgag ataagcatga acagcctcag cttcttcttg
     3301 ggtgaatgat tctgcgaaac tgaccatccc tttgtctgta tacaagcccc ctaagacaat
     3361 ggcattaaat ttactgtggg tgtcttcgtt catgtatctc aagtcagaaa ttacaccacc
     3421 cccaatagcc ccggcaccat ggcaaatgaa acagtgagca tggtatttaa cttcaccatt
     3481 ggcaatcgta atctggtctg catctgattc tggagggtag ggcatttttt tgttgggtgg
     3541 tctatttgga agagaaagat cagcatccaa tgaaaaggta ataattcttc cgttaatggg
     3601 accacctgag tattttggcg gtaagccagc ttccaatgca aaaactcctc cataacccgc
     3661 tagcacggta atgtattgtt
//
LOCUS       DASCTH010000286         1992 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000647603, whole genome shotgun sequence.
ACCESSION   DASCTH010000286 DASCTH010000000
VERSION     DASCTH010000286.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000647603"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="QGH35_06085"
     CDS             <1..743
                     /locus_tag="QGH35_06085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HJL82130.1"
                     /translation="SQIKPHITKRFSMPWSSPAARMKEMILAIQAIWDCWQNGTKLNF
                     RGDFYTHTLMTPFFNPGPNPHGLPKIYVAAVGPLMTQAVAEAADGLLGHPLNTPKYTK
                     EVTMPIVEKALASTGKSRSDFDLSISIMTAIGMTEESYQKAIKACRAQVAFYASTPNY
                     RAVLETHGVGDLSKELNLLSKKGQWQEMGKLIDDDLLNTFAVVAETPEALAAEIKNRY
                     GELGDRITPILYSLEIELAPLVLDALRS"
     gene            complement(761..1546)
                     /locus_tag="QGH35_06090"
     CDS             complement(761..1546)
                     /locus_tag="QGH35_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008491415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJL82131.1"
                     /translation="MEFETVIYEVDNKVATITLNRPDSLNAFNSQMVLDLREATDAAY
                     KDSSIRCVIITGNGKGFSAGADLKENMTQPNDPNAVEKGLLEGYKPVFMNIINMPKPV
                     ISAVNGPAAGIGYSFAMACDLTLMSEEAYLLSAFSNIGVVPDGGANWMLTNAVGYKLA
                     YQLAIEGEKIPASRCLELGLANKVVPSDKLLSEAKDWATKLSQRSPQALSQTKKIMRE
                     ALNSTFEEMFSIEAKIQNQLIGSADNIEAVTAFIEKRAPKFKD"
     gene            complement(1612..>1992)
                     /locus_tag="QGH35_06095"
     CDS             complement(1612..>1992)
                     /locus_tag="QGH35_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HJL82132.1"
                     /translation="DAGYLDEDGFLYIHDRVKDMIISGGENIYPAEVENALMGHPEIA
                     DAAAVGVPDEDWGESVKGFVILAANSSLTEEAIIEYTRGQIAHYKCPKSIHFIEEMPR
                     NPSGKILRKDLRAPFWEDYEGNVS"
BASE COUNT          532 a          450 c          407 g          603 t
ORIGIN      
        1 gatcgcagat caagccacac attaccaagc gtttttcgat gccatggtcg agtcctgctg
       61 ccagaatgaa agaaatgatt ctcgcaatcc aggctatttg ggattgttgg cagaatggca
      121 ctaagctaaa tttcagagga gatttctata ctcacaccct gatgactcct ttttttaatc
      181 ctggtcccaa tccccatggg cttcccaaaa tttatgtcgc agctgtagga ccattgatga
      241 cacaagcagt tgctgaagcg gcggatggtc ttttagggca tcccttaaat acccctaaat
      301 ataccaaaga agtcaccatg ccgatcgtgg agaaagcgtt ggcttcaaca ggaaagagtc
      361 gctcagattt tgatttatca atcagtataa tgaccgcgat tggcatgacc gaagaatcct
      421 atcagaaggc cattaaagct tgccgagcac aggtcgcgtt ttacgcatcg actccaaatt
      481 atcgagcggt actagaaacc catggagtag gagatctcag taaggaattg aaccttttat
      541 cgaaaaaagg acagtggcaa gaaatgggaa aattgattga tgacgatctt ctcaatacct
      601 tcgcagtggt tgctgagact ccagaagcac tcgcagctga aataaaaaac cgttatggtg
      661 aactgggtga tcgtatcacc cccattttgt attctcttga aattgaactg gcacctttgg
      721 ttcttgatgc tctgagaagt taatcgatat ttccagtccg ttagtctttg aatttcggtg
      781 ctcgtttctc tataaaagcc gtcactgcct ctatattgtc agcagaacca atcaactggt
      841 tttgaatttt agcttcgatt gagaacattt cttcaaaggt actgttgaga gcctcacgca
      901 ttattttttt cgtttgactc aaagcctgag gtgatctttg tgatagtttt gttgcccaat
      961 ccttggcttc cgagagcagc ttatcactgg ggactacttt gttggcaagt cccagttcca
     1021 agcatctaga ggctggaatt ttttcacctt caatagctag ctgataggct aatttatagc
     1081 caaccgcatt ggttaacatc cagttggctc caccgtcggg taccactcca atattactga
     1141 atgctgatag caggtaggcc tcttctgaca tcaatgttaa atcacaggcc atggcaaaac
     1201 tgtagccgat tccagcagca ggtccgttaa ctgcagaaat caccggtttt ggcatattga
     1261 tgatgttcat aaagaccggt ttgtagcctt caagcagtcc tttctctacc gcattagggt
     1321 cgtttggttg agtcatgttt tcttttagat ccgcacctgc tgagaagcct ttaccattgc
     1381 cggttatgat aacgcatcgt atggaactat ctttgtaagc tgcatcagtt gcctccctca
     1441 ggtccagcac catttgacta ttgaatgcgt tgagactatc tggacggttg agagtgatag
     1501 ttgcaacttt attatctact tcataaataa ccgtttcaaa ttccatttgt ttatcctttt
     1561 ttttcttaaa ttagcattaa gtttgtagat acaaacacag cagatcttat atcacgagac
     1621 atttccttca tagtcttccc agaagggcgc tcttagatct tttctaagaa ttttacccga
     1681 tgggttacga ggcatttcct caataaagtg aattgattta gggcacttgt agtgggcaat
     1741 ttgacctcta gtgtattcaa tgattgcttc ttctgttagt gaagagtttg ctgcgagaat
     1801 gacaaatcct ttaacactct caccccagtc ctcatccggt actccaaccg cagcagcatc
     1861 cgcaatttca ggatgaccca ttaaagcatt ttccacttca gcagggtaga tattctcccc
     1921 ccctgagata atcatatctt tgactctatc gtgtatatag agaaacccat cctcatcaag
     1981 ataaccagca tc
//
LOCUS       DASCTH010000287         3200 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000656858, whole genome shotgun sequence.
ACCESSION   DASCTH010000287 DASCTH010000000
VERSION     DASCTH010000287.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000656858"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="QGH35_06100"
     CDS             <1..960
                     /locus_tag="QGH35_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HJL82133.1"
                     /translation="NRTEQYNQAVEVFKRALHINYVNDGFYNLDQIDIHDSLTESFIG
                     LKDLDGANHHQEFQVRIYKNHYGTENQEVDTSLQKLANWYKRSGQIFSERLIYEEMYQ
                     RQSNRQNQTIKDLVNTLKNLSFSHRREGLDMYDSVSPLKKALQRLEDDPGNHFRLKLE
                     TLLDLGDTYFSYGKVQSGMQAYQECWELIEKDSSLRTEIENRFSKPVKVRNIRIPTIY
                     SPDDESLENGYYKQGFISIRFDIDTRGRTHNIKIIESDPDGLLDREAELAIKRSIFRP
                     MYIEGEIHPSEGLITRHEFQYYHEEKTIAEPEILDQPLENPMT"
     gene            complement(963..2108)
                     /locus_tag="QGH35_06105"
     CDS             complement(963..2108)
                     /locus_tag="QGH35_06105"
                     /inference="COORDINATES: protein motif:HMM:NF018485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL82134.1"
                     /translation="MTIAFNFKQIAMILVAVTLAGVTFGITMPLSTLTLKSWGIGSAM
                     IGFSAAMPAIAAVLITPFLPKLINYFGQVITIRFCLVLEAVCIFCLPFFPNITSWLFL
                     RFFIGVGATGIWLMSEIWINGYANDKNRGKIIAIYSSLLSAGFIIGVLLVSIIGIESN
                     IAFYAAAGIVAMAAIPMWNITPLPIDDMDEDIAFWDHMFSEPGLMGSSWMMGFLYAAT
                     ASLLPVFALQFDLNYAQSSRTVAWLGSGELFLPLLVGWLADRMNKRKLMIYISLLTVA
                     MLSLLPFIFPSPLLRLVVLFVLGGAIMSFYSLGLTMLGQQFKGSLLASANASFIFFLC
                     LGEIFGPPVIGAAMDFFGTYAFGWSMALFSIIYLLVFISLGRKAQLN"
     gene            complement(2126..3136)
                     /locus_tag="QGH35_06110"
     CDS             complement(2126..3136)
                     /locus_tag="QGH35_06110"
                     /inference="COORDINATES: protein motif:HMM:NF015445.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HJL82135.1"
                     /translation="MKFHSLSIIVILIFILGCDTSQEPTLIELTVGGSAIPNTAGELH
                     FLDFQKSIESQSSDFKLKMLVHSQLGSEENLVSGLRRNRVHFANLSAIITSTLVPEAA
                     LLYAPFLFDNEKEADFVYDNYLVEIFTQMLAEKDLQFISWSEIGFHNVYGKEPILVPA
                     DANSKRYRVSASLSSRYFAEAIGADLIPMGYGEIVQSLQTGLIEAGDNAVALYAKRGI
                     DQEAPHYTQTRHSLGMSIIVARKEWWDQLTIDQRQVVKNAWPTPAISRTSLRAEAVQD
                     LLNAEALGIIVHQLSASDRELWRQATAGQTERLAKVIGGRSLEVLNLIETGKQQFNLI
                     HN"
BASE COUNT         1099 a          658 c          585 g          858 t
ORIGIN      
        1 aaccggactg aacaatacaa tcaagccgtt gaagtattta aaagagcgtt gcatattaat
       61 tatgttaatg acggttttta taatttagat cagatcgaca ttcatgactc cttaacggag
      121 agtttcatag gtttaaaaga tttggatggg gctaatcacc accaagaatt tcaagtcaga
      181 atatataaga atcattatgg cactgaaaat caagaggtgg atacatcgtt acaaaagctt
      241 gccaactggt ataaaagatc aggacagata ttttctgagc gtctcatata tgaggaaatg
      301 tatcaacggc aatccaatcg tcaaaaccaa acgataaaag atcttgtcaa cacgttaaag
      361 aatctttctt tttcccacag acgagagggt ttagatatgt atgatagtgt atctccccta
      421 aaaaaagcat tgcagcgctt agaagatgat ccaggcaatc atttccgttt gaaattagag
      481 actctcctcg atttgggtga cacctatttt agctatggta aagtgcaatc tggcatgcaa
      541 gcctatcaag aatgctggga gctgattgaa aaagattcat cactgagaac agagattgaa
      601 aaccgtttct ctaaaccggt aaaggtaaga aatattagaa tcccaacgat ttattcacct
      661 gatgatgaat ccttagaaaa tgggtattac aaacaaggtt ttataagcat ccggtttgat
      721 attgatacca ggggcagaac tcataacatc aaaattatcg aatcggatcc agatggatta
      781 ttggatagag aagccgaatt agccatcaaa agatcaattt ttaggcctat gtatattgag
      841 ggtgaaatcc atcccagtga aggtttaatt acgagacatg aatttcaata ctaccatgaa
      901 gaaaaaacta tagctgagcc tgaaatcctt gatcagccct tagaaaatcc tatgacttaa
      961 tatcagttca gttgagcctt gcgccctaat gaaataaaaa ccagcaaata aatgattgaa
     1021 aacaaggcca tactccaacc aaaagcatag gttccaaaaa aatccattgc tgccccaata
     1081 accggtgggc caaaaatctc tccgagacac aaaaagaaaa taaaactggc attagctgaa
     1141 gctaacaaac tacctttaaa ttgttgcccc aacatggtta aacctaaact atagaagctc
     1201 atgattgcgc ctccaagaac aaataacact acaagacgca ataaaggaga ggggaaaata
     1261 aaaggtaata acgacagcat tgcaacggtc agcaacgaaa tataaatcat cagttttctt
     1321 ttattcatcc tatctgctag ccaaccgact agaagtggca gaaacagttc tccactgcca
     1381 agccaagcaa ccgttcttga tgattgggca taattgagat caaattgcaa cgcaaatacc
     1441 ggtagtaatg atgcagttgc tgcatataaa aaccccatca tccaacttga acccatcaat
     1501 cctggttctg agaacatatg atcccaaaaa gcaatgtcct catccatatc atcaatgggt
     1561 aaaggggtaa tattccacat gggtatagcg gccatagcca cgattccagc agcagcataa
     1621 aacgcaatat tactctctat accaatgatg gaaaccaaca aaactccgat aataaaccca
     1681 gcggataata aagaggaata aatggctata atttttccac gattcttatc gttggcatat
     1741 ccattaatcc aaatctcact catcagccaa atgccagttg ctcctactcc aatgaaaaac
     1801 cttaaaaata accaactagt tatgttggga aaaaagggca gacaaaaaat gcataccgct
     1861 tctaaaacca agcaaaatct aatggttata acctgaccaa aataattaat taatttaggc
     1921 aaaaaaggtg tgattaaaac agctgcaatc gccggcattg ctgcgctgaa gccaatcata
     1981 gcagaaccaa taccccaact tttaagcgtc agcgtgctta aaggcatggt aataccaaag
     2041 gtgacaccag ccaatgtaac cgcaacaaga atcatggcaa tctgtttgaa attaaaggcg
     2101 atagtcatta ggttgatctg aaaaattaat tatgaattaa attaaactgt tgttttccgg
     2161 tttcgatcaa attgagcact tctaaggacc gtcctccaat tactttagct aagcgttctg
     2221 tttgtccagc tgttgcttgt ctccagagtt cccgatcgga tgctgataat tgatgcacta
     2281 tgattccaag tgcctctgca tttaaaagat cttggacggc ttctgctcga agagacgttc
     2341 tagagattgc aggtgtaggc caggcatttt tgacaacttg cctttgatcg atggttaatt
     2401 gatcccacca ctctttcctt gccactataa tggacatgcc cagagaatgt ctggtttgtg
     2461 tgtagtgcgg tgcttcctga tcgatccctc ttttcgcgta caaagcaaca gcgttgtccc
     2521 cagcctcaat caatcctgtc tgcaaagact gaacaatttc accgtaaccc atggggatca
     2581 gatcagcacc gattgcttca gcaaaataac gagaactgag acttgcagaa accctgtatc
     2641 ttttgctgtt agcatctgca ggcaccagta ttggctcttt cccataaaca ttatgaaacc
     2701 caatttcact ccaagaaata aactgcagat ctttttctgc aagcatctgt gtaaatattt
     2761 caactaggta attgtcataa acaaaatctg cttctttttc attatcaaaa agaaaaggcg
     2821 cgtaaagcaa ggctgcttct ggaaccagtg tagatgtgat gatggctgat aaatttgcga
     2881 aatgaacccg attccttctc aagccagaaa ccaaattctc ttcagaaccc agttgactat
     2941 ggaccagcat tttcaatttg aaatcgctag attgcgattc gatagatttt tgaaaatcca
     3001 aaaaatgtaa ttccccagca gtatttggta ttgccgaacc tcccacagtt agctcaataa
     3061 gagttggttc ttgagatgta tcgcaaccca gaataaaaat tagaataaca attatggaaa
     3121 gtgagtgaaa tttcatatct gattgtaaaa ataaatctga tatattgtct catcagacat
     3181 gatttgcgtc tatcttccaa
//
LOCUS       DASCTH010000288         1935 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000660724, whole genome shotgun sequence.
ACCESSION   DASCTH010000288 DASCTH010000000
VERSION     DASCTH010000288.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000660724"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="QGH35_06115"
     CDS             complement(<1..382)
                     /locus_tag="QGH35_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020583511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HJL82136.1"
                     /translation="MRQIILDTETTGMSVEAGHRIIEIGAVEIVDRRMTGNHYQTYLN
                     PKRSIDPGSIAVHGITNDDVADKPEFKEVMQEFLSFIQDTELLIHNAPFDTAFINHEF
                     SLAGYEKKIEDLCEIKDTLLIARKT"
     gene            complement(379..822)
                     /gene="rnhA"
                     /locus_tag="QGH35_06120"
     CDS             complement(379..822)
                     /gene="rnhA"
                     /locus_tag="QGH35_06120"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HJL82137.1"
                     /translation="MKEVDIYTDGACRGNPGPGGWGVILKNQAATKELYGGDLDTTNN
                     KMELLAAIKGLQSLTQPCKVYLYTDSNYVKQGITEWIINWKRNGFKNAKKKPVLNADL
                     WLELDQLTSIHDIEWFWIKGHSGHPENERADALANKGVDEITINR"
     gene            complement(819..1511)
                     /locus_tag="QGH35_06125"
     CDS             complement(819..1511)
                     /locus_tag="QGH35_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82138.1"
                     /translation="MRPLWLLDTIGSRLIQEEQEELVDVLAISNGDGFLQIGIWGHSK
                     TFTNHLHVDEFFLVEQGVIEGVDLVAKFDRLPIQNQVIDVVFLPHTLEQAKVNQPILN
                     STTELLKDGGKLIAIGFNPFSLWGLRHYLSGKRFLLGMNQLISVKRYTTWLMDLGFGI
                     EKIHYFHATLPTVKRYDLPLKINIFNPFLCACYMIVANKERIPMTLEKQFTRDGYKQS
                     GSLADSMSRVIE"
     gene            1638..>1935
                     /locus_tag="QGH35_06130"
     CDS             1638..>1935
                     /locus_tag="QGH35_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014421393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL82139.1"
                     /translation="MSFLSGKKALITGIASKRSIAYGIAQSMAREGAELALTYQNEKL
                     KPRVENIAEQLGSNICIELDVALEENIDDAFSNLSSHWENLDILIHSIAHAPSEE"
BASE COUNT          595 a          398 c          336 g          606 t
ORIGIN      
        1 gggttttacg ggcaatgagc aaagtgtctt tgatctcaca aagatcctct atcttttttt
       61 cataacctgc caatgagaat tcatggttga tgaaagcagt atcaaaagga gcattatgga
      121 tcagcaactc ggtatcttga atgaagctta gaaactcttg catcacctct ttaaattctg
      181 gtttgtccgc aacatcatca ttagtaatgc catgaactgc gatagaacca gggtcaatac
      241 ttcgttttgg attcagataa gtttggtaat gattgcccgt cattcttcta tccacaatct
      301 cgacagcacc aatttcaatg atcctatgcc ctgcttccac agacatccca gtcgtttcag
      361 tatcgagtat aatttgtctc atcggtttat tgtgatttca tcgacgcctt tattagccaa
      421 tgcgtcagcg cgttcatttt ctggatggcc agaatgacct ttaatccaga accattcaat
      481 atcatgaata gaagtcaatt gatcaagctc taaccacaag tctgcattca aaactggctt
      541 tttctttgca tttttaaacc catttctttt ccaattaata atccactcag tgatcccttg
      601 tttcacataa tttgaatcgg tatataaata gaccttacag ggctgtgtta aactctgcag
      661 acctttaatg gcagctaaaa gttccatttt attattggtt gtatctaaat cgccaccata
      721 taactccttg gtcgcagctt gattttttag tatcacaccc cacccaccgg gcccagggtt
      781 gcctcgacaa gcgccatcgg tgtaaatatc gacttctttc attctattac tctactcatt
      841 gaatcggcta gggagccaga ctgtttataa ccgtcccttg taaattgttt ctccagagtc
      901 attggtatac gttccttatt agcaacaatc atataacaag cacataagaa tggattaaaa
      961 atattaatct ttaaaggcaa atcgtatctt ttgactgtcg gtaatgtggc atgaaaataa
     1021 tgaattttct caattccaaa ccctaaatcc atgagccatg tggtgtatct tttaactgaa
     1081 atcaattgat tcattcccaa taagaatctt ttaccggaaa ggtaatgcct taaaccccat
     1141 aagctgaatg gattaaaccc gatagcaatc aatttgccac cgtctttaag taactcagtg
     1201 gttgaattta aaatcggttg gttgactttg gcctgctcta aggtatgggg taaaaataca
     1261 acatcaatca cctggttctg aataggtaat cgatcaaact ttgctaccag atcgacacct
     1321 tctataacac cctgctcaac gagaaaaaat tcatcaacat gaagatggtt tgtaaacgtt
     1381 ttactatggc cccaaatacc aatctggagg aaaccatcac cattgcttat agccagtaca
     1441 tcaacaagtt cttcttgctc ttcttgaatg agtcgagatc caattgtgtc tagtaaccag
     1501 agcggtcgca tgtcaaaata atcctcataa tattatctga taggataggt tatttttaat
     1561 gtaaaatata ataaatggtg atgcagttaa atatcaacat tacaaatata aatgaaactt
     1621 aatttacgga gagctaaatg agttttctta gtggcaaaaa agctttaatt acgggtattg
     1681 caagcaaaag atcaatagcc tatggcattg ctcaatcgat ggctcgtgaa ggagctgaat
     1741 tggccttaac atatcaaaat gaaaaactga aaccacgcgt tgagaacatt gcagagcaac
     1801 ttggttccaa tatctgcatt gaacttgatg ttgctttaga agagaacatt gatgatgctt
     1861 ttagcaatct tagttctcat tgggaaaatc ttgatattct gattcactca atcgcgcatg
     1921 ctccaagcga agaac
//
LOCUS       DASCTH010000289         1755 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000671681, whole genome shotgun sequence.
ACCESSION   DASCTH010000289 DASCTH010000000
VERSION     DASCTH010000289.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000671681"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="QGH35_06135"
     CDS             <1..562
                     /locus_tag="QGH35_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012515.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HJL82140.1"
                     /translation="LSDINKNVKTALLANIHPALQQTIIDQCNAEMIVLDTMNLWIET
                     TRNLLDTVLKHVTVLLINESEAELLTGISNPQKAGQQLMEYGIKKVVVKLGSHGAKLI
                     DEENQIHIGAYPVKNVIDPTGAGDVFAGTFAAVLNSGGTAKEALVQASALASVCVEGF
                     GVEKILECDKDEINKRISYINTTLGL"
     gene            719..1702
                     /locus_tag="QGH35_06140"
     CDS             719..1702
                     /locus_tag="QGH35_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJL82141.1"
                     /translation="MYLQQNDLEKAEEFLVKAMAIEVDNPEIPFQLGHHIYGKDGRWA
                     EMNDVYNRALEIDPNRKILDGSTVKDMINISRYGYWSDVYNKGINVYNSSKGLDHQDR
                     KSIIRDAVNEFQTALIIKSDESLNYTMLATCFFDLGEVEKSTELIAKAVEFAPEDFNV
                     NMAAGQIFARNDQPENALPYLQKAVELDPSNSLAIRNLAQTYYDLDNTDESIATYEKA
                     IQTTLDAKAKADLHFNLGVLYNQVGDFDAAEDHFVSALDLNPEDVEAIVGMAQTFENN
                     EKWRKAEKFYRELIYLEPENASHYRGIARVLLQQGKQDQAQRYFEKSKKFE"
BASE COUNT          617 a          275 c          360 g          503 t
ORIGIN      
        1 tttatctgat ataaacaaaa atgtaaaaac agctctttta gctaatatac acccagctct
       61 tcagcaaacc attattgatc aatgtaatgc agaaatgatt gtgttggata caatgaattt
      121 gtggattgaa acgactcgga atttattgga tactgtctta aaacacgtaa cggtactttt
      181 aatcaatgaa agtgaagctg aattattaac aggcatttcc aatccgcaaa aagcagggca
      241 acaacttatg gaatatggaa taaaaaaagt tgtggttaaa ctgggaagtc atggtgcaaa
      301 attgattgat gaagaaaacc aaattcatat cggagcatat ccggtaaaaa atgttattga
      361 tcctacaggt gcaggagatg tatttgcagg tacatttgcc gctgttttga actctggtgg
      421 tactgcaaaa gaagctttag tccaggcatc agcattggct tctgtttgcg tagaaggttt
      481 cggagtagaa aaaattcttg aatgtgataa ggatgaaatt aataaaagaa tatcttatat
      541 aaacacgact ctaggtcttt gaaaagcgct ggttactcac tattttctaa acccaaaaat
      601 attataaata tataagagag gacgtatgtt tcaccacaca caaaacaaaa cacttttggt
      661 aatgctgtgc accttcatct tttgggcgtg tcccagcgag gaatacacat cagcaaagtt
      721 gtatttacag caaaacgatt tagaaaaagc ggaagaattt cttgttaaag ccatggcaat
      781 tgaagtagat aatccggaaa taccatttca gctagggcat catatttatg gaaaagacgg
      841 ccgctgggct gaaatgaatg acgtgtataa cagagcactg gaaattgatc cgaatcgtaa
      901 aatccttgat ggcagtaccg ttaaggatat gattaatata tccaggtatg gatattggag
      961 tgatgtctac aataaaggca taaatgtata taactcatca aaaggtttgg atcatcaaga
     1021 tcggaaatcg attattcgag atgccgttaa tgaatttcaa accgctttaa taatcaagtc
     1081 agatgaaagt ttaaattaca cgatgctggc aacatgtttc tttgatttgg gtgaggtaga
     1141 aaaatcaact gaattaatcg caaaagcagt tgaatttgcg cctgaggatt tcaatgtaaa
     1201 tatggcagca gggcaaattt ttgcacggaa tgaccaacct gaaaacgcat taccatatct
     1261 tcagaaagct gtggagttag atcccagtaa cagtttggca attcgtaact tggcgcaaac
     1321 gtattatgat cttgacaata cagatgaatc catagcaact tacgaaaaag caattcaaac
     1381 tactttagat gcgaaagcaa aagctgactt gcatttcaat ttgggtgttt tatataatca
     1441 ggtcggagat tttgatgcag ctgaagatca ttttgtttct gcccttgatc ttaatcctga
     1501 agatgtagaa gctattgttg ggatggctca gacatttgag aacaacgaga agtggagaaa
     1561 agcagaaaaa ttttatagag aattgattta tttagaaccg gaaaacgctt cacattatcg
     1621 tggaattgca agggtacttt tgcagcaggg aaaacaggat caagcccaac gatattttga
     1681 aaaatccaaa aaatttgaat aagaattatt cttacccgtt ttatacaaat tcctcgtgaa
     1741 aacgggggat tattt
//
LOCUS       DASCTH010000290         2680 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000672193, whole genome shotgun sequence.
ACCESSION   DASCTH010000290 DASCTH010000000
VERSION     DASCTH010000290.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000672193"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="QGH35_06145"
     CDS             <1..593
                     /locus_tag="QGH35_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82142.1"
                     /translation="FALIAVPLTTFITWGPAYMARVHNFSMAETGFMLGMILLFLSPA
                     GAYFGGWLIDRLQKLGYADATLRVGLLVAILLLPLSFYATRISNSTMTIVLFCPYVFI
                     MSMPIAAAPTTLQMLVPNQMRAQISAIWMLVLNILSAIAGPTLVGFITTYWLRDDIAV
                     GSSLTLANCLSILIAGLLLWAAIRPFRDALEGQESS"
     gene            complement(607..1488)
                     /locus_tag="QGH35_06150"
     CDS             complement(607..1488)
                     /locus_tag="QGH35_06150"
                     /inference="COORDINATES: protein motif:HMM:NF017206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HJL82143.1"
                     /translation="MKIAVTAASGGLATAMMKYLIDELGTENLCAVARSPEKVKISGI
                     ETRRGDYQSVEELTKAFLVIDTVVMISAPVGDWDRVAMHRNVITAAKQASVRKILYTS
                     VIGNGKEKNTWFWQTQQVNRQTEIDLQESGLEWVVARNGLYLEKDLHHIMQAKDPGVY
                     RNIADNGKTGYITVDELAYATAKLVFNDRANAQIFNLVGDTLTQAQLVHLVNQVFDMD
                     VRYETITDEENIAELMKDPKISDRGEQVAKMLTGVFQAVRAGALDVKSDFRTAAGRAV
                     KPTLQMLQEQRELMEAS"
     gene            complement(1534..>2680)
                     /locus_tag="QGH35_06155"
     CDS             complement(1534..>2680)
                     /locus_tag="QGH35_06155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJL82144.1"
                     /translation="WKNQVISGLTQGLQGLSKKRNINVIQGIASFTSANTVKITNGKD
                     EQALSFEQCIIAVGSNPTTLSFLPDDPRIIDSTDALELSTIPNKMLIIGGGIIGLEMA
                     TIYSSLGTSVSIVELTETLMPGVDRDLVTPLEKYLEKKCDHIYKGAEVSSATSEKEGI
                     KISFQGKSAPESAVYDSVLIAVGRQPNISTINPKGAGVQCDENDFIPVDRQMRTNVPN
                     IFAIGDIVGEPMLAHKASHEAKVAAEVISGMKTAKDSRCIPSVAYTDPEVAWVGLTEE
                     DCKAQGIDFGKGVFPWAASGRSLSIARSEGLTKLLFDPRTKRILGGGMVGTNAGELIA
                     EITLAIEMDCDASDIALTIHPHPTLSETLALAAETFEGTITDLYIKK"
BASE COUNT          697 a          611 c          540 g          832 t
ORIGIN      
        1 ggtttgcatt aatagctgtt cctttaacta cctttattac ttggggtccc gcttatatgg
       61 cacgtgtaca caatttctcg atggcagaaa caggttttat gctcgggatg atattattat
      121 ttttatcgcc tgcaggagct tacttcggtg gttggttaat tgatcggctg caaaagcttg
      181 gatatgcaga tgccacactc agagttggat tactagtagc gatattactg ttgccactta
      241 gcttctatgc gaccaggata tcaaacagca ccatgaccat agttctattt tgtccctatg
      301 tattcataat gagcatgccg attgctgctg ctccaacaac gctacaaatg ctagttccga
      361 atcagatgcg tgcacaaatt tcagcaatct ggatgttggt actaaatata ctttcagcta
      421 tagcgggacc aacactagtg ggtttcatta cgacatattg gttgcgcgac gatatagcgg
      481 taggctcatc ccttacactg gcaaattgtc tcagtattct aattgcaggc ttattattat
      541 gggctgcaat ccgtcccttt agagatgcat tggagggaca agaatcatct tagttgatgg
      601 atgttgctag ctagcttcca ttaactctcg ctgttcttga agcatctgca aagttggttt
      661 aacagctcgt ccagcagcag ttctaaaatc tgatttaaca tcaagtgccc ctgcacgaac
      721 tgcctgaaaa accccagtca gcatttttgc aacttgttca ccacgatcgg agatttttgg
      781 gtctttcatc agttccgcta tattttcttc atcagtaatg gtctcgtaac gcacgtccat
      841 atcaaagacc tggttgacca aatggacgag ttgggcttgg gtcagagtat caccaacaag
      901 gttaaaaatt tgtgcatttg cacggtcatt aaatacaagt ttcgctgtag cataggccag
      961 ttcatccact gtaatatagc cggtcttacc attatcggcg atgtttcggt aaactccggg
     1021 atctttagcc tgcataatat ggtgtaaatc tttctctaga tacaagccat ttctagctac
     1081 cacccattct aacccagact cctgtagatc gatttccgtc tgtcgattga cctgttgggt
     1141 ctgccagaac caggtatttt tctctttgcc gttaccaatg acgctggtat agagtatttt
     1201 acgcacacta gcttgtttag cagcggtaat aacattacga tgcattgcta ctcgatccca
     1261 atcaccaaca ggtgctgaaa tcatcacaac tgtatctatg accaagaagg ccttggtgag
     1321 ttcttcaact gattggtaat cacctcgccg tgtttcgatt cctgagattt ttactttttc
     1381 aggagaccgg gcaacagcac agagattttc ggttccaagt tcatcgatta gatacttcat
     1441 catggcggtt gcaagccccc cggaagcagc ggttacagca atcttcacaa aattactctc
     1501 ttaattaaca ttatttaata aaagcctaac tctttatttt tttatataga gatcagtaat
     1561 cgttccctca aaagtctcgg cagcaagtgc cagagtctca gacaatgttg gatgtgggtg
     1621 aattgtcaaa gcaatatcgg aagcatcaca atccatctca attgcaaggg tgatttctgc
     1681 tatcaattct cctgcatttg tccccaccat gcctccacct agtattcttt ttgtgcgggg
     1741 gtcaaaaagt aatttagtca agccttctga tctagcaatt gagagggatc tcccacttgc
     1801 agcccaagga aaaacacctt ttccaaaatc tattccctgg gctttgcagt cctcttcggt
     1861 caacccgacc catgcaactt ctggatctgt atacgccaca gaggggatgc aacgagaatc
     1921 ctttgccgtt ttcatacccg atattacttc tgctgcaact ttagcttcgt ggcttgcttt
     1981 atgagccagc attggttctc caacaatgtc tccaatggcg aaaatatttg gaacattggt
     2041 acgcatttga cgatccactg ggataaagtc gttctcgtcg cattgtactc ctgctccttt
     2101 tggattaatc gtactaatat ttggttgtct acctaccgcg atcaatacgg aatcatacac
     2161 tgcagactca ggtgcacttt tgccttgaaa agatattttg ataccttctt tctcggatgt
     2221 ggctgaagat acttccgcac ctttataaat atggtcgcac ttcttctcca aatacttttc
     2281 aagtggtgtg acaagatctc tatctactcc aggcatcaaa gtttcggtca gttcgactat
     2341 gctgacactt gttcccaggg aagaatatat agttgccatc tcaagaccaa tgattccacc
     2401 tccaataatg agcattttgt ttgggattgt cgaaagttca agcgcatcag tggaatcaat
     2461 aattctagga tcatcgggta aaaaggagag cgtggtaggg ttggatccca cagcaataat
     2521 acattgttca aagctaagcg cctgttcgtc tttcccgtta gtaattttta cagtgttggc
     2581 ggaggtaaaa gaagcaattc cctggatgac attgatattt ctcttcttgg acaaaccctg
     2641 caatccttgt gttaaaccag aaattacttg gttcttccaa
//
LOCUS       DASCTH010000291         1650 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000680579, whole genome shotgun sequence.
ACCESSION   DASCTH010000291 DASCTH010000000
VERSION     DASCTH010000291.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000680579"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="QGH35_06160"
     CDS             complement(<1..346)
                     /locus_tag="QGH35_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJL82145.1"
                     /translation="MTEHPIEIHMFPCLSDNYGFLVHDPQSQQTATFDTPESTTIEEA
                     LTTKGWELTHIFNTHHHYDHAGGNLELKEKTNCLILGSSLDQKRIPGIDVTLEDQQIF
                     HFGSHKIHCHHTP"
     gene            complement(363..893)
                     /gene="moaB"
                     /locus_tag="QGH35_06165"
     CDS             complement(363..893)
                     /gene="moaB"
                     /locus_tag="QGH35_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HJL82146.1"
                     /translation="MSKTKQDGREFVPINIAVLTISDSRTKENDTSGDILAQRIQDAG
                     HNVAERGICKDDIYKIRAIVSQWFVNQEIHAIITTGGTGITGHDGTPEAILPLLDKVI
                     DGFGEVFRSLSFQNIHTSSLQSRALAGVANGTYIFCLPGSNGACKDGWDLIISFQLDY
                     RTLPCNLVELMPRLQE"
     gene            complement(960..1514)
                     /locus_tag="QGH35_06170"
     CDS             complement(960..1514)
                     /locus_tag="QGH35_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJL82147.1"
                     /translation="MDDDIDIGTRLKKLRAEKSISQRQLAIKAKVSNAAISLIENNKA
                     NPSLGILKKILDSVPISISDFFAMDIQIETKVVFRKHELTEIGAGAISYLQVGKNLQN
                     NQLQMMLERYKPGADTGQNMLTHESEESGIILEGRLEVTIGDNVYYLNAGDAYLFNST
                     IPHRFKNPDAIDCVLISACTPPSF"
BASE COUNT          473 a          298 c          308 g          571 t
ORIGIN      
        1 ctggggtatg gtggcagtgt attttatgac taccaaagtg aaatatttgt tggtcctcga
       61 gtgtcacatc aataccaggt attctcttct gatctaggct agaacccaag attaaacagt
      121 ttgttttttc tttcagctcc aaattaccac cagcgtggtc atagtgatga tgggtattga
      181 aaatatgggt tagctcccat ccttttgttg tcaacgcttc ttcaatcgta gtactttcag
      241 gcgtgtcaaa ggttgccgtt tgttggcttt gcgggtcgtg aacaagaaat ccatagttat
      301 cggataaaca gggaaacata tggatttcta tggggtgctc tgtcattact ggtttatgga
      361 ttctattctt gcagtcttgg catcagttca accaaattac aaggcagggt tcgatagtcc
      421 aattgaaagg aaatgattaa atcccaacca tccttgcagg cgccatttga tcctggcagg
      481 caaaaaatgt aggttccatt agccacaccg gctaaagctc ttgactgcaa agaagaggta
      541 tgaatatttt gaaacgacag cgatcggaac acttcgccaa aaccatcaat cactttgtct
      601 aaaagcggta ggattgcttc aggggtgccg tcatggcctg ttattcctgt gccaccggtg
      661 gtaataattg catgaatctc ttgatttaca aaccattgag aaacaatggc ccttatttta
      721 taaatatcgt ctttgcaaat tcctctctcg gcaacgttat gaccagcatc ttgtatacgt
      781 tgtgcaagaa tatcaccaga agtatcattc tccttggttc tactatccga aatagttagc
      841 acggcaatgt ttattgggac aaattccctt ccatcttgtt ttgtttttga cataaattaa
      901 gcttctttgt tttctagtat tttatttcag atttgttttg taatctattg ttaattgacc
      961 taaaaactcg ggggagtaca agctgaaatg agaacgcaat ctatcgcatc tggattttta
     1021 aaacggtgtg gaatagtact attgaataaa taggcatctc cagcatttaa gtagtaaaca
     1081 ttatctccaa tagtcacttc caaacgacct tctaaaatta tgccactttc ttcagattca
     1141 tgagtgagca tattttgccc agtatctgcg cctggtttgt atctttctaa catcatttga
     1201 agttgattat tttgtaggtt ttttccgact tgaagatagg atattgctcc tgcaccaatt
     1261 tcagtgagct catgttttcg aaaaacaacc ttagtttcta tttgaatatc catggcaaag
     1321 aaatcgctga ttgagatcgg tacggaatcc agtatctttt ttaagatacc aagtgatggg
     1381 ttggctttgt tgttctcgat taatgaaata gctgcattac tgacctttgc tttgatcgcc
     1441 aattgtcttt gtgaaataga tttttctgct cttagttttt taagcctagt gccaatatca
     1501 atgtcgtcgt ccataatgtt aaattgttaa ttatgttaaa caaaaaatat aaaaacctat
     1561 taaaaacaat tatttataaa aattcaattt aaatattgtt aaatttataa aaaatcttta
     1621 tggtgtgatt gttatttaat taataatcta
//
LOCUS       DASCTH010000292         2050 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000690144, whole genome shotgun sequence.
ACCESSION   DASCTH010000292 DASCTH010000000
VERSION     DASCTH010000292.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000690144"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="QGH35_06175"
     CDS             <1..186
                     /locus_tag="QGH35_06175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HJL82148.1"
                     /translation="NVIKNISIAIGPEGGFNRDEKNMAEKSGYRIIKTGPRLLRTETA
                     PIMALSILQYLYGDFSN"
     gene            219..1175
                     /gene="gshB"
                     /locus_tag="QGH35_06180"
     CDS             219..1175
                     /gene="gshB"
                     /locus_tag="QGH35_06180"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF003573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HJL82149.1"
                     /translation="MSNRKLAVIMDPISGIAPEKDGTLGLLLEAQSRSYDLIYFEQQD
                     LRIDNGMAIGDGCHLAVKDSSTDWFKLSEKNRISLADLDVVLMRKDPPFNTEYIYTTY
                     VLDLAESSNVLVINSPQSLRNFNEKVSITQFPECAPPSLVTSSIENLKNFLGQHKKIV
                     VKPLDSMGGKSIFVITEGDLNANSILETITKENMESVMAQLYIPEIKNGDKRIHLING
                     KHADILLARIPLDIDNRGNLDAGAQAKTQTLSENDKHICNRIGPELNARGVFFAGIDV
                     IGDFLTEINITCPTGMREIKQHADYDVVSDLFDAIEEQLTGG"
     gene            1183..1734
                     /locus_tag="QGH35_06185"
     CDS             1183..1734
                     /locus_tag="QGH35_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HJL82150.1"
                     /translation="MKTNLVGQFLIASPSMNDPHFHQTVTYICENNEAGSLGFIINRP
                     MKASLSDILNQLDLENSQSLANFPILQGGPVGLERGFVLHQEGTWDNTSAIINGLSVT
                     TSKDILKSISSGLGPSESLLILGYSGWGPGQLEQELKTSSWINAPANNEILFTVPYKN
                     RWQKAINLLGIEASDISDQIGHA"
     gene            1768..>2050
                     /gene="ruvX"
                     /locus_tag="QGH35_06190"
     CDS             1768..>2050
                     /gene="ruvX"
                     /locus_tag="QGH35_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HJL82151.1"
                     /translation="MHLKPDSVLIGFDYGTKKTGVAIAQRITQTATALNIIEMKNGCP
                     QWSQLDQIINDYKPDLAIIGTPGRENLQTSKLVDKIQNFASVIEKHYTMP"
BASE COUNT          697 a          362 c          404 g          587 t
ORIGIN      
        1 aatgtaataa aaaatatttc aatcgccata ggtccagaag gaggttttaa tcgtgatgag
       61 aaaaatatgg cagaaaaatc tggttataga attattaaaa ctggacccag actattgaga
      121 acagagactg cacccattat ggctttgagt atcctccaat atctttatgg agattttagt
      181 aattagttta gaatgacata aaaaaataat gtaaatctat gagtaacagg aaactagcag
      241 tcataatgga tccgatatca gggattgctc cagaaaaaga cggaacgctt ggtctgttgt
      301 tggaagccca atctcgatcc tatgatttaa tttattttga acaacaggat ctcagaatag
      361 acaacggtat ggccataggt gatggctgtc atttagctgt taaagatagt tcaaccgatt
      421 ggttcaaatt atcagagaaa aatagaattt cattggctga tcttgatgtc gttctgatga
      481 gaaaagaccc accttttaat actgagtata tctataccac ctatgttctt gatctggcag
      541 agtcatcgaa tgtcctcgtg attaacagtc ctcagtcttt aagaaatttt aatgagaaag
      601 tcagcatcac acagttccca gagtgtgctc caccctcttt ggtaacgtcg tcaattgaga
      661 acttaaaaaa tttcttaggg cagcataaaa aaattgtggt caagccgcta gatagcatgg
      721 gtggtaaatc aatttttgtg attaccgaag gtgacctcaa tgccaattct attcttgaaa
      781 ccatcaccaa agaaaacatg gaatccgtga tggctcaatt gtacattcct gaaataaaga
      841 atggggataa gagaatacat ttaatcaatg gaaaacatgc agatatcctg cttgctcgta
      901 ttcctttgga catagataat cgtggaaact tagacgcggg agcccaagct aaaacccaaa
      961 ctcttagtga gaatgataaa catatttgta atcgtattgg tccagagtta aatgcccgtg
     1021 gcgttttctt tgcagggata gatgtgattg gtgatttcct aacagaaatc aatataacct
     1081 gcccaactgg catgagggaa ataaaacaac atgcagatta tgatgtggtc agtgatcttt
     1141 ttgatgccat tgaagaacaa ttaacaggtg gataaatcca atttgaaaac caatttagtg
     1201 ggacaatttc ttattgcatc accgagtatg aatgatccac attttcatca aacagtaact
     1261 tatatttgtg aaaacaatga ggctggatca ttggggttca ttatcaacag accaatgaag
     1321 gcaagtttgt cggatatatt aaatcaattg gacctagaga attctcaatc attagctaat
     1381 tttcccattc ttcaaggagg tccggtaggt ttggaacgag gctttgtctt gcatcaggaa
     1441 ggaacatggg ataacacatc tgcaatcatt aatggtcttt ccgtaacgac ttctaaggat
     1501 atattaaagt ctatatcatc aggacttgga ccctctgaat ctttgcttat tctgggatac
     1561 tctggatggg gacctgggca attggagcaa gaattaaaaa ccagttcatg gattaacgca
     1621 ccagcaaata atgaaatatt gtttactgta ccttataaaa atcgatggca aaaagccatt
     1681 aacctattgg gcattgaagc ctcagacatc agtgaccaga taggtcatgc ttaaccaata
     1741 aaagctcttc ttacaactaa aataataatg catttaaaac ccgatagcgt tctcattggt
     1801 tttgattatg gaacaaaaaa aacgggggtt gccatcgcac agcgtattac acaaactgca
     1861 acagctctaa atatcattga gatgaaaaat ggatgcccac agtggagtca gctggaccaa
     1921 attatcaatg attataaacc tgatttagcc attattggaa cacccggtcg agagaattta
     1981 caaacttcta agttagtcga taaaatccaa aattttgcgt ctgtgataga aaaacattat
     2041 acaatgccca
//
LOCUS       DASCTH010000293         1850 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000712278, whole genome shotgun sequence.
ACCESSION   DASCTH010000293 DASCTH010000000
VERSION     DASCTH010000293.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000712278"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="QGH35_06195"
     CDS             <1..738
                     /locus_tag="QGH35_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HJL82152.1"
                     /translation="NDGKIQHAKGNALQPIKPIYNNTRGFYGERSLSNQKNPNNTNEV
                     YIDRDIIVLANPEIAQLPPWIIALVAAGALAAALSTAAGLLLVISTAVSHDLIKQTFA
                     RNISEKKELMFARISVLFSVIIAGLFGIYPPAFVAQVVAFAFGLAASTLFPVIFLGIF
                     SKTMNKEGAITGMLSGLIFTLTYIIYFKFIHPELNLPDYWLWGISPEGIGFIGMLLNV
                     IIGTLVSLMTPKPPTEVDLMIDNIRRP"
     gene            743..1531
                     /locus_tag="QGH35_06200"
     CDS             743..1531
                     /locus_tag="QGH35_06200"
                     /inference="COORDINATES: protein motif:HMM:NF018649.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HJL82153.1"
                     /translation="MSILHMLSYLILVNWICLTMLWLISLKIKDASIIDIYWGFGFVI
                     MSWTGLIINLFFNETTITTSQWLINIMVTIWGLRLSIHLAIRNLGKGEDLRYVKMRER
                     ASSNWRFLSYIRVFMFQGFLQMLLMTPIVLVHYYPGQFGMSFLDYFGLLVWAIGFGIE
                     AITDMQLTKFRSNATNSNKILKTGLWRYSRHPNYFGDALQWFAFFIISLNSGYIWGVI
                     GPILMVFIFLKLTIGILEKSQTRKRPGYEEYTKSTNVFFPGKRG"
     gene            complement(1555..>1850)
                     /locus_tag="QGH35_06205"
     CDS             complement(1555..>1850)
                     /locus_tag="QGH35_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HJL82154.1"
                     /translation="RSDSVICLPGGVGTWEEFFESLTWNQLGLQSKPIVLLNIEDYYS
                     TLAEFIEHSVLEGFLPRSTASELIFVDSIPAAIEHIENYIPKDKSNWFKRLQE"
BASE COUNT          527 a          325 c          377 g          621 t
ORIGIN      
        1 aatgatggca agattcagca tgcaaaaggc aatgccttgc aaccaatcaa accgatttat
       61 aacaataccc gtggatttta tggagagaga agtcttagta accaaaagaa tcctaataat
      121 acaaacgaag tatatattga tagggatatc attgtgcttg ctaatccaga aattgctcaa
      181 ttacctccat ggattatcgc tttggtagca gctggagccc tagcggcagc actttcaact
      241 gcagcgggct tgttattggt catttctact gcggtttcac acgatttaat caaacaaact
      301 tttgcacgaa atatttcaga aaaaaaagag ctaatgtttg ctcgaatttc ggtattattt
      361 tcggtcatca tcgcagggtt atttggcatt tacccacctg catttgttgc tcaggtagtt
      421 gcctttgcat ttggattggc agcatcaact cttttcccag tgatttttct gggaattttt
      481 tcaaagacga tgaataagga aggagctatc acgggtatgc tctctggttt aatttttacc
      541 ttgacctaca ttatttattt taaattcatt catcctgagt tgaacctacc tgattattgg
      601 ctttggggta tttcaccaga aggtattggt ttcattggaa tgttattgaa tgtaattatt
      661 ggtaccttgg tctcattgat gaccccaaag cctccaactg aagttgattt gatgattgat
      721 aatattagga gaccctagag caatgtctat tctgcatatg cttagttatc tcatattagt
      781 caattggata tgtttgacta tgttgtggtt gattagttta aaaatcaaag atgccagcat
      841 cattgatatt tattggggtt ttggatttgt cattatgtct tggactggtt taatcattaa
      901 tctattcttc aatgagacta caataaccac aagccaatgg ctgattaaca tcatggtgac
      961 gatctggggt ctgcgtttat cgattcacct tgcgattagg aatttgggca aaggagaaga
     1021 tctacgatat gtaaaaatga gagaacgagc atcatcaaac tggagatttc tcagctatat
     1081 aagagttttt atgtttcagg gttttttgca aatgctcttg atgactccga ttgtattggt
     1141 ccattattat ccggggcagt ttgggatgag tttcttggac tattttggtc tgttagtttg
     1201 ggctatcgga tttggaattg aagcaattac tgatatgcaa ctaactaagt tccgctccaa
     1261 tgctacgaac agtaataaaa tattaaagac aggactctgg cgttattcca ggcatcccaa
     1321 ttactttggt gatgcattgc aatggtttgc tttctttatc atttcactga attcaggtta
     1381 catttgggga gttattgggc ccattttaat ggtttttatt ttcctgaaac tcaccatagg
     1441 catattggag aagtctcaga caagaaagcg acccggctat gaagaataca ctaaatccac
     1501 taatgtgttc tttccaggca agagaggcta gcaattaata tcaaagagct gggattattc
     1561 ctgtaaacgt ttgaaccaat ttgatttatc ttttggtatg taattttcaa tatgttctat
     1621 tgctgctgga attgagtcaa caaatatgag ctcacttgca gttgatcgag gaagaaatcc
     1681 ttccaaaaca gagtgttcaa tgaattctgc cagtgttgaa taatagtctt ctatgttgag
     1741 cagtacaatc ggtttcgatt gcaaacccag ttggttccat gtcagtgact caaaaaattc
     1801 ttcccatgtt ccaacaccac cgggtaggca aataaccgaa tcagatcgtt
//
LOCUS       DASCTH010000294         3375 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000719004, whole genome shotgun sequence.
ACCESSION   DASCTH010000294 DASCTH010000000
VERSION     DASCTH010000294.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000719004"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="QGH35_06210"
     CDS             complement(<1..242)
                     /locus_tag="QGH35_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010561420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HJL82155.1"
                     /translation="MKKIVLNNLIIVGLLFGIVFADLTVAHSLESIIAGEHRSDTNKA
                     RDAYRHPQQTLEFFGLEEDMTVVEIWPSGGWYAEVIA"
     gene            complement(242..943)
                     /locus_tag="QGH35_06215"
     CDS             complement(242..943)
                     /locus_tag="QGH35_06215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HJL82156.1"
                     /translation="MVIPLGDENPTHSKPIVSIAILIICCLVFLWQFSLGSDGGKAIY
                     ALGVIPASLIQGIELPAEIQWVSPGMTLITSMFLHGGFMHLGGNMLYLWIFGDNVEDI
                     LGKFPFILFYLVCGIVAALTQALPEPGSQIPMIGASGAISGILGAYVVFFPKHKVKVA
                     IPFGFFIQILRLPAYVVLLFWFVLQLFNSASAGASGGVAFRAHIGGFVAGLVLGPVFA
                     VVTRRTREKLKGENE"
     gene            complement(943..1779)
                     /locus_tag="QGH35_06220"
     CDS             complement(943..1779)
                     /locus_tag="QGH35_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013594.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HJL82157.1"
                     /translation="MIKRRHCWILSGTLFVSMMYSPFAQTALLVSEREIEIESAKQWE
                     QMINSVPISRDLGKRTLVRCVSRRIIKLIDEPYASLDWEIEVFEDPDANAVVLSAGKI
                     GVNTGLFEVASNQNELAAVIGHEIAHVTNKHIYERAKRSKRTQIGGLIGSAVVASKIA
                     SQPIYTQADLYETQNKINAINTMSQVLETYGLNLPYAREQESDADKTGIILMAQAGFN
                     PIATMTLWKKMKQQDKEKRVPEFASTHPSSSNRINGLASQLSDALAVYNKVEKKPNCG
                     YR"
     gene            complement(1782..2762)
                     /gene="lipA"
                     /locus_tag="QGH35_06225"
     CDS             complement(1782..2762)
                     /gene="lipA"
                     /locus_tag="QGH35_06225"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HJL82158.1"
                     /translation="MVIENEKLGNLSGEKYTNQFGITAIKDGMKKTSDASLNSRLRKP
                     EWLKVQLPTGKHYKTLLQDVKAHGLNTVCQESKCPNIGECWNRSTATLMILGNICTRA
                     CRFCAVDTGNPKGWIDPLEPNNVAETVSLMALDYVVLTSVDRDDLPDGGARHIAHCVT
                     QTKAKNPKVLIEVLSPDFSGSTASLDVLLQSTLDVFSHNIETVKRLTSLVRDPRASYH
                     QSLDVLMHAYENNIITKSGMMLGLGETDDEIITTLRDLRSVGVSLMTLGQYLQPTKYH
                     LDVARFVSPVEFNRYKEIGLGMGFKEIVAGPLVRSSYRAEQSFLNLLNQI"
     gene            2844..3323
                     /locus_tag="QGH35_06230"
     CDS             2844..3323
                     /locus_tag="QGH35_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014678.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HJL82159.1"
                     /translation="MKLAKNIIGIFILVLLIILPKGEAQYSINERFIGWKQQPIGIKS
                     NGKIININAYIANTQTKRKQGLMHVESMPDDVGMLFIFDPPRMVSIWMKNTLIELDII
                     FIDQDQKIIKIHKNAQPYSLESISSGLAVKWVVEINGKASDLWEIKPGDQLIFQQAD"
BASE COUNT         1006 a          742 c          635 g          992 t
ORIGIN      
        1 gcaataacct cagcatacca acctccagag ggccatattt caactacagt catatcttcc
       61 tctaagccaa aaaattccaa ggtttgttga ggatgcctgt atgcgtctct agctttattg
      121 gtatccgatc gatgctcacc agcgataata ctttccagtg aatgggcaac agttaggtct
      181 gcgaaaacta ttccaaacag taaccccacg ataattaaat tattcaatac tatttttttc
      241 attattcatt ctctcctttc aatttttccc ttgtcctcct ggtcacaacc gcaaatacag
      301 gcccaagaac taaaccagct acaaaaccgc caatgtgtgc acggaaagcc acaccgccac
      361 ttgctccagc gctggcacta ttgaacaatt gtagaacaaa ccaaaacaac aatactacat
      421 aagcaggcaa acgaagtatt tggataaaaa atccgaacgg gattgccact ttaactttat
      481 gttttggaaa gaacacaaca tatgctccca atatacctga aatagcaccg ctggcaccaa
      541 tcataggtat ttgtgagcct ggttctggca gcgcttgcgt cagagctgcg acaataccac
      601 aaactaaata aaacaggatg aatgggaact tgcctaatat atcttcaacg ttatcaccaa
      661 aaatccagag atagagcata tttccaccta agtgcataaa cccgccatgc aaaaacatgg
      721 aggtgatcaa ggtcatacct ggactcaccc attgtatctc tgcaggtagc tctatcccct
      781 gaataaggct tgctggaata acccctagtg cataaattgc tttaccacca tctgacccta
      841 aactaaattg ccataggaaa acaagacaac aaataatcag gattgcaatg ctgacgatcg
      901 gttttgaatg cgtcggattt tcatctccta gtggaataac cattatctat aaccacaatt
      961 gggttttttc tcaactttgt tatagaccgc cagggcatca gaaagttggc tagcaagacc
     1021 attgattcta ttggacgatg aaggatgtgt gcttgcaaat tcaggtaccc ttttttcttt
     1081 atcttgctgt ttcatttttt tccatagagt catcgtggca attggattaa atcctgcctg
     1141 agccatcaaa atgatgcctg tcttatcagc atcagattcc tgttccctgg cataagggag
     1201 attcagaccg taggtttcta aaacctgtga cattgtattg atagcattaa ttttattctg
     1261 agtctcgtag agatcagctt gtgtatagat gggttggcta gcaattttac tggccaccac
     1321 tgcagaaccg attagacctc ctatctgagt ccgctttgat cttttggctc tctcataaat
     1381 gtgtttgttc gttacatggg ctatttcatg accgatgaca gcagccaatt cattctggtt
     1441 gcttgcaacc tcaaaaaggc ctgtgttgac tcctatctta cctgctgata agacgacggc
     1501 attggcatct gggtcttcaa atacttctat ttcccagtct agactggcat agggttcatc
     1561 gatgagctta ataattcttc ttgaaacgca acggactaaa gttctctttc ctagatctct
     1621 tgatattggc acagagttaa tcatttgctc ccattgtttt gccgattcga tttcgatctc
     1681 tcgctcgctg accaagagag ctgtctgggc aaatggtgaa tacatcattg atacaaacag
     1741 ggtacctgag agaatccaac agtgtctgcg cttgatcata attagatttg gttcaaaaga
     1801 tttaaaaagg actgttctgc acggtagctt gatcgcacca aaggtcctgc aacaatttct
     1861 ttgaagccca tacccaatcc aatttcttta taccgattaa attcaactgg cgatacaaag
     1921 cgagcaacat caagatggta ctttgtgggt tggaggtatt gacccaatgt cattagactg
     1981 actccaacgg aacgaagatc tctcaaggtg gtaatgattt catcatcagt ttcaccaaga
     2041 ccaagcatca tgcctgattt agtaattata ttgttctcat aagcgtgcat gagtacatcc
     2101 aaagattgat gatagctagc ccttgggtcc ctgacaaggg aagttaatcg tttgactgtt
     2161 tcaatattat gagaaaaaac atccagtgtg ctttgcaaca gtacatcgag tgatgctgtt
     2221 gatcctgaaa aatcaggaga cagaacttca atcaacacct taggattttt agcttttgtt
     2281 tgagtcacac aatgtgcaat atgtcgggca ccgccatcgg gtaaatcatc tctatcgacc
     2341 gaggtcagta ccacataatc aagcgccatc aaagaaactg tttcagcaac attattgggc
     2401 tccaatggat caatccaacc ctttggatta ccggtatcca ctgcacagaa tcggcatgct
     2461 cgagtgcata tgttgcccag tatcattagt gttgccgtgc tacggttcca acattctcct
     2521 atatttggac atttagattc ctggcatacg gtatttaaac catgggcttt gacgtcttgc
     2581 agtaacgttt tataatgctt tccggttggc aattgaacct tgagccattc tggtttccta
     2641 agacgactat tcagtgaagc atcagaggtt tttttcatcc catccttaat tgcagtgatt
     2701 ccaaattgat tggtatattt ttcaccgcta aggtttccca gtttttcgtt ttcaatgacc
     2761 acagtaatta atatctctta acaattgcac tattctctat acttcacctc aattcagttt
     2821 aagatgtttt ctatatgatg aaaatgaagc tagctaaaaa cattataggc atattcattc
     2881 ttgttctctt aataattttg ccaaaaggag aagcccaata ctcgattaat gaacgcttca
     2941 ttggatggaa gcaacaaccc attggcataa agagcaacgg caagataatc aatatcaatg
     3001 cctacattgc taatactcag accaagcgaa aacaaggact catgcatgtc gaaagcatgc
     3061 cagacgatgt aggaatgctg tttatctttg atccacccag aatggtttca atatggatga
     3121 aaaatacact catcgagctt gatattattt ttattgacca ggatcaaaaa attatcaaaa
     3181 ttcacaaaaa tgcacaaccg tatagtttgg aaagtatttc atcagggtta gcggttaaat
     3241 gggtggttga aatcaacggt aaagccagcg atctatggga gatcaagcct ggagatcaat
     3301 tgatttttca acaagcagat taaagagggc cgcgccttag aatcgtccac catctggcaa
     3361 tattaatcaa actca
//
LOCUS       DASCTH010000295         1824 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000720132, whole genome shotgun sequence.
ACCESSION   DASCTH010000295 DASCTH010000000
VERSION     DASCTH010000295.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000720132"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="QGH35_06235"
     CDS             <1..279
                     /locus_tag="QGH35_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HJL82160.1"
                     /translation="FSKVLLIEYPRKGLYSLSFQTSQDLGEVQGKTGEDLVCVFVPTT
                     PNPTSGLILMVPKQDVIELDMGVEDALKMVISLGVVVPGEGTVNQNIE"
     gene            300..>1824
                     /gene="aspS"
                     /locus_tag="QGH35_06240"
     CDS             300..>1824
                     /gene="aspS"
                     /locus_tag="QGH35_06240"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009150647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HJL82161.1"
                     /translation="MNIEDSIRTKYCGELNISNVGEIISVAGWVHRRRDHGGVIFVDL
                     RDTSGLLQLVFNPEDKDLFSQAEKFRSEFVLSVTGIIRKRPEGTINPEMPTGEIELLA
                     QSGHVLNVSKTPPFHHDEHANEDVRLRYRYLDLRRSEMANKLKQRHAVIRSLRNFLDN
                     HDFVDIETPILTKATPEGARDYLVPSRIQKGEFFALPQSPQLFKQLLMMSGFDKYYQI
                     ARCFRDEDLRADRQPEFTQLDVEMSFVNEEEVLDIMEKMIAKLFQDVLSENLTDPFLR
                     ISHRESMERFGTDRPHLGISMELIDVSKLMKQVEFKVFSEPANAENSRVACLKLENAG
                     EISRKQIDDYTDFVSGFGARGLAYIKVQDISKGMDGLQSPILKFLDKETIQTLMESLT
                     LASGDIVFFGAGKSNIVNASLGALRVKLAQDFDQVQPGWFPLWVTDFPMFQMDEKENK
                     WSAEHHPFTAPKEITSKELINNPSEAMSRGYDLILNGHEIGGGSIRIHSLEMQKAVFE
                     "
BASE COUNT          574 a          315 c          413 g          522 t
ORIGIN      
        1 ttcagcaaag ttttattgat cgaatatccc agaaaaggat tgtatagctt gtctttccag
       61 acatcacaag atttaggtga agtacaagga aagacgggtg aagatttagt atgtgtgttt
      121 gtacctacaa caccaaatcc aacttcaggc ttaatattga tggtacccaa gcaagatgtt
      181 attgaattgg atatgggtgt tgaagatgcc ttaaaaatgg tcatttctct tggcgtggtg
      241 gtccctggtg aaggcaccgt caatcagaat attgaataat tgattacttc ggagattggg
      301 tgaatattga agattcaatc agaacgaaat actgcggtga gctgaatata tccaatgtag
      361 gtgagatcat ctcagttgcg ggctgggtac accggagaag ggatcatgga ggagtgatct
      421 ttgttgatct tagggatact tcgggcttac tgcaattggt gtttaatcct gaagataaag
      481 atttattttc ccaagcagag aagtttagaa gtgaatttgt tctatccgta actgggatca
      541 ttcgcaagag accggaagga accattaatc cagagatgcc aacgggtgag attgaattac
      601 ttgcccaatc tggtcatgtc ttaaatgttt caaaaactcc cccatttcat catgacgagc
      661 atgcaaatga agatgttcgt ctcaggtatc gatatcttga tttgagacgt tcggaaatgg
      721 caaacaaact caaacaaaga catgcagtta tccggtcatt gagaaatttt cttgataatc
      781 atgactttgt agatattgaa accccaatac tgacaaaggc aaccccagag ggagcaagag
      841 actatttggt tccaagcaga atccaaaaag gcgaattttt tgcactgcct caatcacccc
      901 aactgttcaa acagttatta atgatgtctg gctttgataa atactatcaa attgctcgtt
      961 gtttcagaga tgaagattta agagcagatc gtcaacctga attcacacaa cttgatgttg
     1021 agatgtcttt tgtcaatgag gaagaagtct tggacattat ggaaaaaatg atagccaaac
     1081 tttttcaaga tgttttatcc gagaatctaa ctgatccctt tctccgcatt agccatcgtg
     1141 aatcaatgga acgatttgga acggacaggc cacatttagg aatctctatg gaattgattg
     1201 atgtcagtaa gttaatgaaa caggttgaat ttaaggtttt ctctgaacct gccaatgctg
     1261 aaaacagcag agtggcctgt ttaaagctag agaatgcagg agagatcagt agaaaacaaa
     1321 ttgatgacta taccgatttt gttagtggat tcggcgcaag gggtcttgct tatattaagg
     1381 tgcaggatat atcaaaagga atggatggct tgcaatctcc aatcctaaag ttcttagata
     1441 aagagactat acagacacta atggaaagct tgactcttgc atctggcgat atcgtctttt
     1501 ttggtgctgg caaatccaac attgtgaatg catctctcgg agctctaagg gtaaagttgg
     1561 cgcaagattt tgatcaggtt caacccggat ggtttccttt atgggtaact gattttccca
     1621 tgtttcaaat ggatgagaag gaaaataaat ggtcagctga acaccatccc tttacagcac
     1681 caaaagagat aacatcaaaa gagttgatca acaacccaag tgaggctatg tcccgtgggt
     1741 atgatttgat actaaacggt catgagattg ggggaggatc gattagaatt cattcactgg
     1801 aaatgcaaaa agcagtcttt gaag
//
LOCUS       DASCTH010000296         2125 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000722108, whole genome shotgun sequence.
ACCESSION   DASCTH010000296 DASCTH010000000
VERSION     DASCTH010000296.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000722108"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="QGH35_06245"
     CDS             <1..541
                     /locus_tag="QGH35_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82162.1"
                     /translation="LNPHPLTAWEVDDIKAPNLLDRFMFWLLNNKNNTIKPKQKKIKP
                     KAITRQKNTHQNKRQRPNNKKSSLKRSSPTKSSPKKTSIKKTSSTKETKKTKETKKKP
                     VQKKAVKKDTNSKSVKKTPKKAATSKIEKHSTKTKADQSSKPKQLITDQSFSKDKVNK
                     TKDDQDKILPWEDILPPRK"
     gene            complement(545..1075)
                     /locus_tag="QGH35_06250"
     CDS             complement(545..1075)
                     /locus_tag="QGH35_06250"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020408750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HJL82163.1"
                     /translation="MWSEQLWQSTKIVMHDLLQSKHKILFVCTGNICRSPSAEGVFRK
                     MIEEHESFDSSIYIDSCGTHSYHIGESPDGRAQHAAANRGIDISKQKARQIEADDFDF
                     FDYILVMDNFNLNVIHSMVDETDRSKIHLFLDYAELNEYKEVPDPYYGGERGFEIVLD
                     LLEQASIGLIKHLQSN"
     gene            complement(1044..1775)
                     /gene="kdsB"
                     /locus_tag="QGH35_06255"
     CDS             complement(1044..1775)
                     /gene="kdsB"
                     /locus_tag="QGH35_06255"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HJL82164.1"
                     /translation="MSQVDFIIVIPARFDSERLYGKALLNLNGKSLIEHVYCAALKSD
                     AKGILIATDSNQIKEVTENFDAKTIMTKKTHASGTDRINEVAELECWEEDQIIVNLQG
                     DSPLMPAENINQVAQLLFDRPDAGIATLATKILDPQELEDPNVVKVEFDKSARATSFD
                     RRVESKNNQMYWRHLGIYAYRVGTLKSFSQHERSEEEVAARLEQLRAFDLNIEIVIQE
                     ASIIPGPEVDTESDLNVVRAIMAKH"
     gene            complement(1762..>2125)
                     /locus_tag="QGH35_06260"
     CDS             complement(1762..>2125)
                     /locus_tag="QGH35_06260"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HJL82165.1"
                     /translation="LLDATAVNHNGTVNKSISEFSGSEVYLLAGISNPERLLQILEEN
                     GISVIQIEIDDHGKVDLSVIREDTNTPILMTSKDAVKYDLQLHQNCWIMEPEVEFDDS
                     YHLPEFIQTLVRKNHESG"
BASE COUNT          721 a          429 c          311 g          664 t
ORIGIN      
        1 cttaaaccca catcctctta cagcttggga agtcgatgac attaaagcac caaatctatt
       61 ggatcgtttt atgttttggc tactaaataa taaaaacaat acaataaaac ccaaacaaaa
      121 aaagatcaaa cctaaagcaa taactagaca gaaaaatact caccaaaata aacgacaaag
      181 acctaacaat aaaaaatcgt cactgaagag atcttcaccg acgaaatcgt ctccaaagaa
      241 aacttcaata aagaaaactt cttcgacaaa agaaaccaaa aagacaaaag aaaccaaaaa
      301 gaaacctgta cagaaaaagg cggttaaaaa agatactaat agtaaatcag taaagaaaac
      361 tccgaagaaa gcagcaacat ctaaaataga aaaacattct actaaaacta aagctgatca
      421 gagttcaaaa cctaagcaac tgataacaga ccaaagtttt agtaaagaca aggttaacaa
      481 aactaaagat gatcaagata agatacttcc ttgggaagac atacttcctc caagaaaata
      541 gatactaatt ggattgtaag tgtttaatca agcctattga tgcttgttct agaagatcca
      601 agacaatctc gaatcccctt tctccaccgt aataaggatc aggaacctcc ttgtattcgt
      661 tgagttctgc ataatctagg aaaagatgaa ttttactcct atcagtttca tcaaccatag
      721 agtgaattac gtttaaatta aaattatcca tcactagtat atagtcgaaa aagtcaaaat
      781 catctgcttc gatttgtctt gccttttgtt ttgaaatatc aatgcctcta ttcgcagctg
      841 catgttgagc tcttccatca ggtgattcac caatgtgata actatgggtt ccacacgaat
      901 caatatatat tgagctatca aaactttcgt gttcttcaat cattttccta aaaacacctt
      961 ctgctgatgg tgatctacaa atatttccag tacaaacaaa aagaatttta tgtttggatt
     1021 gcaatagatc gtgcatcaca attttagtgc tttgccataa ttgctctgac cacattcaga
     1081 tcagattcag tatcaacttc aggaccagga ataatgctcg cctcttggat gacgatctct
     1141 atatttaaat caaacgctct caattgctcc aatcttgctg ctacttcctc ttcacttctc
     1201 tcatgttggc taaaagattt caaagtccct accctatagg catatatacc aagatgcctc
     1261 caatacattt ggttgttttt gctttcaact ctcctatcga atgaggttgc cctagcagat
     1321 ttgtcaaatt caaccttcac cacatttgga tcttcaagtt cttgtggatc taaaattttt
     1381 gttgccagag tggcaattcc agcatcaggt ctatcaaata ataattgagc tacctggttt
     1441 atattttctg ctggcatcaa tggactatct ccttgtaaat taacaataat ttgatcttct
     1501 tcccaacatt ctaattcagc gacttcattg attctgtctg tgccagaagc atgtgttttt
     1561 ttagtcatga tagtttttgc atcaaaattt tctgtgactt cctttatttg attactgtct
     1621 gttgcaatca atatcccttt tgcatcactt ttcaacgcag cacaataaac atgttcaatg
     1681 agtgatttgc catttaaatt taataaagct ttcccgtata gacgttctga atcaaatctt
     1741 gcagggataa caataataaa atcaacctga ctcatggttc tttctcacta gtgtttggat
     1801 aaattctggt aaatgatagc tatcatcaaa ctcaacttcc ggttccataa tccagcaatt
     1861 ttgatgcaat tgtaaatcat atttcacagc gtctttagag gtcattagga ttggtgtatt
     1921 ggtatcctct ctgattactg ataaatcaac ctttccatga tcatcaattt caatttgaat
     1981 aacagagata ccattctctt ctaagatttg taataacctc tcagggttgc tgattccagc
     2041 aagtaaataa acctctgatc ctgaaaattc actgatcgat ttattgacag taccattgtg
     2101 attgactgct gttgcatcta gtagt
//
LOCUS       DASCTH010000297         2749 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000724980, whole genome shotgun sequence.
ACCESSION   DASCTH010000297 DASCTH010000000
VERSION     DASCTH010000297.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000724980"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="QGH35_06265"
     CDS             complement(<1..212)
                     /locus_tag="QGH35_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82166.1"
                     /translation="MNKIKIIASLILLHTTIIFSHTDGYDGQELSTNKIDEQHLHQHI
                     QILASDEFEGRAPGSEGGEKTKEYLV"
     gene            complement(232..1002)
                     /locus_tag="QGH35_06270"
     CDS             complement(232..1002)
                     /locus_tag="QGH35_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012596.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HJL82167.1"
                     /translation="MHKYKYLQVKKRLGVTNVFLNRVDNRNALNEGLMVELTDFCSEI
                     RKDIKTKVIVFRSNAKDFSVGADLKQSRSDQSKLYQWKHNRGKDLIQSILEIEQITIC
                     LMQGYCLGGAAVIASACDFRIADENTKVGYPEINLGMSLNWLGLPLAINLVGVARAKQ
                     MVIGGDFHDANELLSWGFIDEVFANENQETCLRKWVKKYSFKPPLAAQMIKRSSNELA
                     MTLFKGIMHMDYEQFMLTMDSADQKKAISKFLTKTKKK"
     gene            complement(1062..1427)
                     /locus_tag="QGH35_06275"
     CDS             complement(1062..1427)
                     /locus_tag="QGH35_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013304.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HJL82168.1"
                     /translation="MQTLILFSLLLALLHIWLLPMILNVKNAEYLLSNRDGVVEESGM
                     SGRVRRASTNFYESFPAFLVLCLLSIMLEVDNISLAMCWLIFRVLFLISYAAGIIYLR
                     TLLWMGSIVCLIMMAVSFI"
     gene            complement(1599..2471)
                     /locus_tag="QGH35_06280"
     CDS             complement(1599..2471)
                     /locus_tag="QGH35_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82169.1"
                     /translation="MSFFSRAVLSLFSFLISASVLANQPAFSRPESTYFYTSGSADPG
                     VYFISSTNGDDVGWVSKLAADGSMIDQRWATDLRNPMGMRVSGKRLWVNNATEVVGIN
                     LDDPSDRIIHLIDGAIVLNDLATDSSGYAYLSDSVNSRVVRVDLATGENSTYISTLPS
                     SPNGLLVQGDKLYIASWGIMSEQPEERAEWITKTAGDLYWVSLKDSKKVRHIIAPELG
                     NLDGVEIDQQGNIYVSDWESGKLYKISSSNKTVIELGQYGQGLADLGLNLLTGELVLP
                     VTLSSEVLFYTPSP"
     gene            complement(2476..>2749)
                     /locus_tag="QGH35_06285"
     CDS             complement(2476..>2749)
                     /locus_tag="QGH35_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HJL82170.1"
                     /translation="GGSFLEPVYTASWPTGEFGGMGLEGAVKLGFKKELDAEENSEKK
                     EALYNTLVAKMYEAGKATEAASHLEIDAVIDPMDTRSLILKALQAL"
BASE COUNT          846 a          593 c          442 g          868 t
ORIGIN      
        1 tttacaagat attcttttgt tttctcccct ccttcacttc caggagctct cccttcaaat
       61 tcatcggatg caagtatttg tatgtgttga tgtaggtgtt gctcatcaat tttatttgtc
      121 gacaattctt ggccatcata accatcggtg tggctgaaga taatggtcgt gtgcaataaa
      181 atgagtgatg cgatgatttt aatcttgttc atattattaa ctctcaataa tttatttctt
      241 tttagtcttg gttagaaatt tgctaatcgc ctttttctga tctgcagaat ccatagtgag
      301 catgaattgt tcataatcca tgtgcatgat ccctttaaaa agagtcatag ctaattcatt
      361 tgaactgcgt tttatcattt gtgcagctaa aggtggtttg aatgaatatt tcttcaccca
      421 ttttctaaga caggtttctt gattctcatt tgcaaaaact tcatcgataa aaccccaaga
      481 taatagttca ttggcatcat gaaagtcacc gccaataacc atttgcttag ccctagcaac
      541 tccgactaag ttaattgcca aaggcagacc caaccagttt aagctcattc ctaaattgat
      601 ttctggataa ccaactttag tattctcatc agcaattcta aaatcacaag cagaagcaat
      661 aacggctgcg cctcccaaac aataaccctg catcaagcaa attgttattt gctcaatctc
      721 taagattgat tgtatgagat ctttgcctct gttgtgcttc cactgatata gctttgattg
      781 atctgatcta gattgtttaa gatctgctcc gactgaaaaa tccttggcat ttgatctgaa
      841 aacaataact tttgttttaa tatcttttct aatttcagag caaaaatcag tcagttcaac
      901 cattaaccct tcattcagag cgttgcggtt atctactctg ttaagaaaaa catttgtcac
      961 gcctaatctc tttttgactt ggaggtattt gtatttatgc atttttcttc ttatcccatc
     1021 gcattaacaa actcaataac tagatatata gataatttag tttaaatgaa agaaactgcc
     1081 atcataatca agcaaactat agaacccatc cataaaagtg ttcttagata aataattcct
     1141 gcagcatacg agattaaaaa taggactcta aaaataagcc agcacatagc cagcgagata
     1201 ttatccactt ctagcattat agaaagaaga cataaaacta aaaatgcagg gaagctttcg
     1261 taaaaatttg ttgatgctct ccttactctc ccagacattc cagactcttc cactacccca
     1321 tctctgttag aaagcaaata ttccgcattc ttgacattta agatcattgg taataaccaa
     1381 atgtgtaaaa gtgccagcaa caatgagaaa agaattaagg tttgcatgat taactccatt
     1441 aatagattta actttattct aaagacttta cggtcataac aagtctactg caagataaga
     1501 ccataataat ttattcccac tcaataataa tatctattta agttattgat ttaattgagt
     1561 attagttcga ctttgtatca agtttacatc aattataatt aaggactggg agtgtaaaat
     1621 aaaacttcgc tactgagcgt aacaggtaaa accaattctc ccgtgagcaa attcaaacct
     1681 aaatcagcca aaccttgccc gtattgacca agttcgatga ccgttttatt gctgctagaa
     1741 atcttgtaga gcttgccgct ttcccaatca gaaacataga tatttccttg ttggtcaatt
     1801 tctacaccat ctaggttgcc aagttctggg gcaataatat gcctcacttt ttttgaatcc
     1861 ttcaaagaaa cccaatacaa atcgcctgct gtcttggtta tccactcggc acgctcctct
     1921 ggttgctcag acatgattcc ccaactggca atataaagct tgtctccttg gaccagcagt
     1981 ccattgggag aagatggtaa ggtggaaata taagtgctgt tttctccagt cgccaaatct
     2041 actctaacca ctcggctatt cactgaatct gaaaggtagg catatcctga agaatcagtg
     2101 gccaaatcat tcaaaacgat tgcaccatct atcaggtgaa taattcgatc actgggatca
     2161 tccaaattaa taccaacaac ttcggtagca ttattgaccc acaagcgttt accagaaacc
     2221 ctcattccca tcgggttccg aagatccgtt gcccagcgtt ggtcgatcat actaccatct
     2281 gccgccagtt tgctcaccca acccacatca tcgccgttgg tacttgaaat aaagtataca
     2341 cctggatctg cactacccga ggtataaaaa taggttgact ctggccgtga aaatgctggc
     2401 tgattggcca atactgaggc agaaattaag aatgaaaaaa gactcagaac tgctctcgaa
     2461 aaaaaactca tagatttata aagcttgaag agctttaaga attaagcttc tggtatccat
     2521 tggatctatt actgcatcaa tttcaagatg agatgctgct tcggtggcct tgcctgcttc
     2581 atacattttc gcaaccaatg tattgtataa agcttccttt ttttctgagt tttcttcagc
     2641 atctaattcc tttttaaatc ctaatttaac agctccctcc aagcccattc cgccgaattc
     2701 tccagtcggc caagaagcag tataaacggg ttctaaaaaa ctccctcca
//
LOCUS       DASCTH010000298         2153 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000738166, whole genome shotgun sequence.
ACCESSION   DASCTH010000298 DASCTH010000000
VERSION     DASCTH010000298.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000738166"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="QGH35_06290"
     CDS             <1..495
                     /locus_tag="QGH35_06290"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HJL82171.1"
                     /translation="HIPPVSGVNISLDLIERLVIRYPNTIVGVKDSSGNWENTQSILE
                     RQLDDFRVFAGSEHFLLQTMRAGGAGCISATANINPNAIYNLYKEWKSNQADDLQKQL
                     NQVRATLESYPMIPALKSVVSYYSNHPDWTIVRPPFISLEKETQKELIQKLKSLGFVM
                     PNLD"
     gene            553..>2153
                     /locus_tag="QGH35_06295"
     CDS             553..>2153
                     /locus_tag="QGH35_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HJL82172.1"
                     /translation="MRKLLKWIGFLFATAFILGMLYLGNLFLMKPFSLDHYLGKELIL
                     ELFDSPEAQTYLGIVDRFNWATGHQSRISITGLEDLEEDLIEAKEAKSMLLAYKDASL
                     SKQQKITKKIAVFDLNNTIEESEKFPYHSYPLNQIGGIHLNIVEFMTDIHPIRNVKEA
                     EAYIDRLNLFDDSFRASLEILEAQRKAGIYPPRFVFDHVIRQLTEFLNYKNEENPLRK
                     VFVRKIKTLELDTETSEDLFLKLDSAIENSVQPGFELLLAFMNETLPQANINHGVWSL
                     PDGDDFYALRLKVYTTTDYSAEEIHNIGFKEVQRITSRMQEILSQLGYGNDLNVGQLM
                     NELNEDSKFLYSDTPDRKELVVQDYSDIVEETWRVAEDYFHKMPQSKVEVRAVPEYSE
                     QNQAGGYYMSPALDGSRPGVFYANLYDIKQTPTYSMRTLAFHEAIPGHHLQNALNLEN
                     EDLSLYRRFGYYTSAFGEGWALYAERLSLEIGLANNPYDELGVLQSELFRAVRLVVDT
                     GIHFKRWTREEAMDYMKKVTGMSDT"
BASE COUNT          711 a          404 c          446 g          592 t
ORIGIN      
        1 catataccac cagtttcagg tgtgaatatt agcctagatc tcattgagcg tcttgttatt
       61 aggtatccca atacaatagt aggggttaag gacagctcag gtaactggga gaacactcaa
      121 agcatactgg agcgtcaatt ggatgatttt agagtgtttg cagggagtga acattttctg
      181 ctgcaaacca tgagagctgg aggtgctgga tgtatttctg caaccgcaaa catcaatcca
      241 aatgcaatct acaatctcta taaggaatgg aaatctaatc aagcagatga tctacaaaag
      301 caattgaatc aggttcgcgc taccttggaa tcctatccaa tgattccggc actgaaatcg
      361 gttgtatcct attacagcaa ccatcctgat tggacaatag tccgaccccc attcatttct
      421 ttagaaaaag aaacccaaaa agaactcatt caaaaactca agagtcttgg gttcgtaatg
      481 cccaatcttg attaaaaaac acacaagacg agactgaaga gaaaaaaaag tagaaaatgg
      541 agtagaaatg atatgagaaa attacttaaa tggattggct ttttatttgc tacagcgttc
      601 atcctaggaa tgctgtattt gggaaattta tttcttatga aacctttttc cctggatcac
      661 taccttggta aagaacttat tctggaactg ttcgactcac ctgaagctca aacctatcta
      721 ggcatagtgg atcggttcaa ctgggctaca ggtcatcaat ctagaatttc tattacagga
      781 ttggaggacc tagaggaaga tctaatcgag gcaaaagaag ccaaatcaat gttgcttgcc
      841 tataaagatg cttcactttc aaagcaacaa aaaataacca agaaaattgc tgtttttgac
      901 ctcaataata ctattgaaga atctgagaaa ttcccctatc acagctaccc tttgaaccaa
      961 atagggggca ttcatttaaa catcgttgaa ttcatgaccg atatccatcc catcaggaat
     1021 gtaaaagaag ccgaagccta tattgataga ctcaatttat ttgacgactc atttagagca
     1081 tctttggaaa ttctagaggc tcaaagaaaa gcaggtatct accctccaag atttgtattt
     1141 gatcatgtca tcagacaact cactgagttt ctgaactata agaatgaaga aaacccactg
     1201 agaaaggtct ttgttagaaa aattaaaaca ttagaattag acacagaaac ctcagaagat
     1261 ctgtttttga agctggattc agcaattgaa aacagtgtcc agcctggatt tgagctgttg
     1321 cttgcattta tgaacgaaac cctaccccaa gccaacatca atcacggggt atggtcttta
     1381 cctgatggag atgattttta tgccctcaga ctaaaggttt atacgacaac cgattattca
     1441 gcggaggaaa ttcacaatat tggattcaaa gaagtgcaaa ggattactag tcgcatgcaa
     1501 gaaattctca gtcagttggg ttatggcaat gatctaaatg taggtcaatt gatgaatgaa
     1561 ctcaatgagg attctaaatt cctgtactca gatactccgg ataggaaaga attagtagtc
     1621 caagattatt ctgatattgt tgaagagacg tggagggtag cggaggacta ttttcataaa
     1681 atgcctcaat caaaagtaga ggttagagca gttccagaat actcagagca aaaccaagct
     1741 gggggatatt atatgtcgcc agcattagat gggagtcggc cgggtgtttt ttatgccaac
     1801 ctttatgata tcaagcaaac cccaacatac agcatgcgta ctctcgcttt tcatgaagct
     1861 attccaggac accatttgca aaatgccctc aacttggaaa acgaagatct atccttgtat
     1921 agaagatttg gatattacac ttcggcattt ggcgaagggt gggcacttta tgccgagaga
     1981 ctgtctttag aaattggcct tgccaataat ccctatgatg aattaggggt tttgcagagt
     2041 gagttattta gagctgtcag attggttgtt gataccggaa ttcatttcaa gcgctggaca
     2101 cgagaagagg ccatggatta catgaagaaa gtaacaggta tgtccgatac aga
//
LOCUS       DASCTH010000299         6321 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000745565, whole genome shotgun sequence.
ACCESSION   DASCTH010000299 DASCTH010000000
VERSION     DASCTH010000299.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000745565"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="QGH35_06300"
     CDS             <1..926
                     /locus_tag="QGH35_06300"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJL82173.1"
                     /translation="GSGATTATVAPAIAPDCSHVTVLQRSPTYFIAGANESKIANFMR
                     KLGFKEKWVHAITRGFILFLQQNLVRRSFKEPEKVKEEFIEGVREQLEPGYDVETHFT
                     PKYRLWQQRLAFVPDGDLFEGIKAGKASMVTDEIENFTEKGILLKSGKELEADIIVTA
                     TGFNLSVLGGIPFSIDNQTVDVAKSVTYRGMMLTGVPNMVWIFGYFRASWTLRVDIVG
                     DFVCRLLKDMKKRGVKSIAPALRSEDKDMPILPWLDPENFNPGYMLRSMDLLPQRGDK
                     SEWQHTQDYWLEKKELRKVDFQDSIFICKYK"
     gene            complement(962..1360)
                     /locus_tag="QGH35_06305"
     CDS             complement(962..1360)
                     /locus_tag="QGH35_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82174.1"
                     /translation="MIKKLNIFFGILLATNAWSNEQTMPVEGLSCSADDPDKLVELWS
                     LDSRSKTVSYWSRNDFKFREFPTKRFDQKIIAWEQQSDFNLVYVLDRTTMRQSGTKLF
                     IDKNGGLNIEKRWVSQCQILTLELLNKLIE"
     gene            complement(1357..2208)
                     /locus_tag="QGH35_06310"
     CDS             complement(1357..2208)
                     /locus_tag="QGH35_06310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL82175.1"
                     /translation="MEIINKRVVVTGAASGIGKALALAFKQADARSVVVVDINTDGVE
                     KIAKEIDGIAITADVAKEKDIEKVIQKANASAGGIDIFCSNAGIGGTPGFVEVESSDW
                     QHIWEVNVMSHIFAAKHLIPQMLEQGGGYLVNTASAAGLLSQPGIASYAVTKAAAVSF
                     AEWIKITFGKRGIGVSCLCPQGVRTPMLTEPFDDLVGVDGIIEPEVVAEDTLDAIRNE
                     KFLITPHEEVLNYIKYKASDRDGWINSMQKFQEQFEESFSALKDFQDLRTTEMENQLE
                     STSNKKE"
     gene            complement(2260..2350)
                     /locus_tag="QGH35_06315"
     tRNA            complement(2260..2350)
                     /locus_tag="QGH35_06315"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2314..2316),aa:Ser,seq:tga)
     gene            2605..3615
                     /locus_tag="QGH35_06320"
     CDS             2605..3615
                     /locus_tag="QGH35_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJL82176.1"
                     /translation="MDIDIILEADLTAQQIKELGLMAEKFGFRAIWTQNYAKARDAFM
                     TAVPLALASKKIMVGVCIVSPYEMHPLKIANATLTLNECAKGRGCVTIGGGGEWMMML
                     GFDTSKRVSATREALEIIQGAIREDTFNYNGKLYKARWFNSDWTTDQPPLIYAGASGP
                     KMLSMSSGVADGVMMGDIAPVMFDWPLPTLNKALADNNRTDDNFRISNFLAWHIKEDR
                     EVAIQEARREMIVRGWLERPWLEPFLSPEDTQFVLDNKWPFIDAYRKRSSHIEGIPEH
                     ILEALVEGLGCTGDPSDLDRYIEKLNKYSEAGFTEVALRIHDDPDYSIKLIGELVLPK
                     LH"
     gene            3615..4622
                     /locus_tag="QGH35_06325"
     CDS             3615..4622
                     /locus_tag="QGH35_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJL82177.1"
                     /translation="MQLDIALESKIGPTAIKELGLLAETYGIRTVWIQNLARAPDAFM
                     AAVPLALASSTIKVGIAVVCSHEMHPLKIANAVLTLNEYAKGKAGVVVTSGGEWPGVV
                     GKKIAEVARTKEALEIIKASLGNEPNVSYEGEFYKAQMFNTKWKTQSPPTLYGGAHRA
                     KMMQMIATTCDALMFSDIQPEMFDGILGTLNTVRPIEDRPNDFRLSNFIAWHIKENRE
                     ASLWEAQRELIIRAWLYREWIEPYLEPEEVEWVLKNPFPFLKAYRERTGDIEGVSQHI
                     VDQLVEGLTMSGDPSDIDRHIERLEAFKREGFTEISLGLQEDPVESIKMIGEQVLPAI
                     Q"
     gene            complement(4675..5961)
                     /locus_tag="QGH35_06330"
     CDS             complement(4675..5961)
                     /locus_tag="QGH35_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010646042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HJL82178.1"
                     /translation="MSSKTLTLIAIFLIPSVGFANELSAGDTSWILTSTALVLFMTLP
                     GLALFYGGLVRSKNIISVLMQCFTIACLVSVVWSIYGYSLAFKGDGKLFGDFSALFLS
                     GVGRDSISGTIPETVFVMFQMTFAIITPALVVGAFAERMKFSAVCLFSLFWITFVYLP
                     ACHMVWGGGFLGTAGVIDFAGGLVVHLTCGVGALIVALVLGPRQGFPKIPMPPHNRTM
                     VVIGAAMLWVGWFGFNAGSAVAADSSAGMAMLVTHISAATGALTWMSIEWIKQGKPTV
                     VGIATGMVAGLATITPAAGTVGPSAAFLIGFLAGLICFYSTQIIKGTFKIDDSLDVFP
                     VHGVGGILGVVMLSLLGSPEGFLGEGASGIAEGGVLVQLKIQLLGIVVIFAWTAVATW
                     IILKIVNVVTGLRIDPESESEGLDVTSHGERGYNIE"
BASE COUNT         1921 a         1348 c         1248 g         1804 t
ORIGIN      
        1 ttggctcagg tgcaacaact gctaccgttg cccctgcaat agctcctgat tgcagccatg
       61 tcacggtgct tcagcgttct ccaacttatt ttattgcagg tgccaatgaa tcgaagattg
      121 caaattttat gcgtaagcta ggctttaaag agaaatgggt tcatgccata acacgcggtt
      181 ttattttatt ccttcagcaa aatcttgttc gtcgctcgtt taaagaaccc gaaaaagtaa
      241 aagaagaatt catagagggt gtacgggagc aattggagcc tggctatgat gttgaaaccc
      301 actttacccc taaataccgg ctctggcaac aacgcctagc cttcgtacca gatggtgatc
      361 tttttgaagg aatcaaggcg ggtaaagcat ctatggtcac cgatgagatt gaaaatttta
      421 ccgagaaagg cattcttctt aaatctggta aagaactgga agccgatatt attgtcacag
      481 caacaggatt taatctcagt gttttaggag gtattccctt ctctatcgat aaccagactg
      541 ttgatgtagc taagtcggtc acctatcgag gcatgatgtt aacaggtgta ccaaatatgg
      601 tttggatctt cggttacttt cgagctagct ggactttacg ggttgatata gtaggtgact
      661 ttgtatgccg tcttctaaaa gacatgaaaa aaagaggtgt taaatcgatc gctccagccc
      721 tccgttcaga agataaggac atgcccatat tgccttggtt agatccagaa aatttcaatc
      781 caggttatat gttgcgttct atggacttgt tgccacaacg aggagataag tcagaatggc
      841 agcatacaca agattattgg ttggagaaga aagaattacg aaaagtagat ttccaagatt
      901 caatatttat ttgcaaatat aagtaagatc tctaccaagc ctgtccaagt gagttctgct
      961 gctactcaat aagcttattg agtaattcca aagttaatat ctgacattgg gaaacccacc
     1021 gtttttctat gtttaacccg ccattcttat caatgaatag cttcgtacca ctttgtctca
     1081 tggtagtcct atcgagaaca tagactaaat taaaatctga ttgttgttcc caggctatga
     1141 ttttttggtc aaatcttttc gtcgggaatt ctctaaactt aaaatcattc ctactccagt
     1201 aagacactgt ttttgaccgg ctatccaatg accatagttc aaccaattta tctggatcat
     1261 ctgcactaca acttaacccc tcaactggca tagtttgttc attgctccag gcattagtgg
     1321 ctaatagaat tccaaagaat atattgagtt tttttatcac tctttcttat tagaagtgct
     1381 ttccaactga ttttccattt ctgtggttct aaggtcttga aagtctttca aagcagaaaa
     1441 gctctcttca aattgctctt gaaatttttg cattgaattg atccatccgt ctcgatccga
     1501 tgctttatat ttgatgtaat taagaacttc ttcatggggc gtgataagaa acttttcatt
     1561 tctaatggca tccagtgtgt cttcagcaac aacttcgggt tcgataatac catctactcc
     1621 aaccagatca tcaaagggtt ctgttaacat cggtgttcta actccttgag gacataaaca
     1681 cgacactccg atacctctct tgccaaaagt aatttttatc cactcggcaa aactcactgc
     1741 cgctgctttc gttacagcat aactcgcgat tcctggttgg cttaaaagcc cagctgcaga
     1801 tgcagtattg accaaataac cacctccttg ttctaacatt tgaggaataa ggtgtttggc
     1861 agcgaaaata tgtgacatga cattcacttc ccaaatatgt tgccaatctg atgattctac
     1921 ctcaacaaaa cctggggtac ctccaatgcc tgcattcgaa caaaatatat cgattccccc
     1981 tgctgaggcg tttgctttct ggatcacttt ttctatatct ttttctttgg ccacgtctgc
     2041 tgtgatcgct atgccatcaa tttctttagc gattttttca acaccatcag tatttatatc
     2101 cactacaacc actgacctag catcggcttg tttaaatgcc aaagctagag ctttaccgat
     2161 gccacttgct gcaccggtta caacgactct tttattgatg atctccatag actttctaaa
     2221 attgaaactt ttgtttgatt actattctac tctttgatct ggcggagagg gagggattcg
     2281 aacccccgga cgaggattac ccgtcaatgg ttttcaagac caccgcattc gaccgctctg
     2341 ccacctctcc ttttatcttt aaaaccctat tctatagtgt ctctggaaga tttaaaactg
     2401 ttatcattta tcagctgaaa ccctctttgg tcgcactttg gtcgcattat ttccaaactg
     2461 ctctatcagg tcactcatga tgtaatttac ttaattaatg gcttttttat gattaggatt
     2521 aaaacacaga aattgattta gatcattttt tatatgattt agatcaattt caaaaccaat
     2581 atcaatctat accattgata agcaatggat attgatatta ttctagaagc agatctaaca
     2641 gcccagcaga ttaaagaact gggtctgatg gcagaaaaat ttggtttccg tgcgatctgg
     2701 actcaaaact acgcaaaagc tagggatgcc tttatgacag cagttcccct cgcattagcc
     2761 tctaaaaaga tcatggtagg agtctgtatc gtcagtccct atgaaatgca cccgttgaaa
     2821 attgcaaatg ccaccctgac acttaatgaa tgtgcaaagg gcagaggttg tgtgactatt
     2881 ggtggaggtg gtgaatggat gatgatgctc ggttttgata ctagcaaaag agtctctgca
     2941 acccgagaag cattggagat cattcaaggt gcaatccggg aggacacctt taactacaat
     3001 ggaaaattat ataaagcaag atggttcaac tctgactgga caactgatca accaccattg
     3061 atctatgccg gtgcctctgg tccaaaaatg ttatctatgt cctcaggggt agctgatggt
     3121 gtaatgatgg gggatatcgc tccggtgatg tttgattggc cattgcccac tttaaacaag
     3181 gccctggcag ataataatag aacggatgat aatttccgta tcagtaactt tctggcctgg
     3241 catatcaagg aagaccggga ggttgcaatc caggaagcca gacgtgaaat gatcgttcga
     3301 ggttggttgg aacgtccgtg gctggaacca ttcctgagtc cagaagacac gcagtttgtg
     3361 ctggacaata aatggccatt tatagatgct tatcgcaaaa gatccagtca tatcgagggt
     3421 attcctgaac atattttaga agctcttgtc gagggtctag gatgcacagg ggatcctagt
     3481 gatctcgatc gttatattga aaaactcaac aagtactcag aggcaggatt tacagaagtg
     3541 gcactgagaa tccatgacga tcctgattac tcaattaaat tgattggtga gttagttctg
     3601 ccgaaactgc actgatgcaa ctggatatag cactagagtc caaaattggc cccaccgcca
     3661 tcaaagagct aggtctgctt gctgaaacct acgggatacg tactgtctgg attcaaaatc
     3721 ttgcaagagc accggatgca tttatggcag cggtgcctct ggcactggcc tcaagtacga
     3781 tcaaagtcgg catagctgtg gtctgttccc atgaaatgca cccactaaaa attgctaatg
     3841 cagtactgac cctgaatgaa tacgccaaag gaaaagctgg tgtagttgtg acttctggag
     3901 gtgaatggcc tggggttgtt ggtaaaaaaa tcgcagaagt tgcacgcact aaagaggcct
     3961 tggagattat caaagcatct ttgggtaatg agcctaatgt gagctatgaa ggagagttct
     4021 ataaagcaca aatgttcaac accaagtgga aaacccaatc accaccaacg ttatatgggg
     4081 gggctcaccg ggctaagatg atgcagatga tcgctaccac ttgtgatgca ctcatgttca
     4141 gtgatatcca acctgaaatg tttgatggga tcctgggtac actgaacaca gtacgtccaa
     4201 tagaggatag acccaatgat tttagattga gtaatttcat tgcctggcat atcaaggaaa
     4261 atcgagaggc atccctgtgg gaggcacaac gtgaactgat tattcgtgcc tggttatacc
     4321 gtgaatggat tgagccttac ttagaacctg aagaggtaga atgggtactg aagaatcctt
     4381 tcccattcct taaggcatat cgtgagcgaa ccggtgatat tgaaggggtt tctcaacaca
     4441 tcgttgacca gctggtcgaa ggtctgacga tgtcagggga tcccagtgat attgacagac
     4501 atattgaacg gcttgaagca ttcaaaagag aaggttttac agaaatctcg cttggcttac
     4561 aagaagatcc agtcgaatca ataaaaatga ttggtgagca ggtattgcca gctatccagt
     4621 gataactcta aaatattttt ataaaatagg tattaagtat taaataacaa attattattc
     4681 gatattgtaa cctctttctc catggctggt aacatccaaa ccttcgctct ctgattctgg
     4741 atcaattcta agacctgtga ctacgttaac tatctttaaa atgatccatg ttgctactgc
     4801 ggtccatgca aaaataacga caatgcctaa aagttggatt tttaactgaa ctaaaactcc
     4861 tccttcagca atgcctgatg caccctctcc gagaaaaccc tctggactac ctaaaaggct
     4921 aagcatgaca acacctaaga ttcctcccac accatgtaca ggaaaaacat caagagaatc
     4981 atcaatttta aatgttcctt tgattatctg ggtagaatag aaacaaatta agcctgctaa
     5041 aaaaccaatt aaaaaagccg ccgaaggtcc tactgtgcca gctgcaggcg ttatggtagc
     5101 taatcctgca accattccag tagcaattcc aacaacggtc ggtttgccct gtttaatcca
     5161 ctcaatggac atccatgtta gagcacccgt ggcagcacta atatgagtaa ccaacatggc
     5221 cattcccgca ctagaatctg cagcaacagc acttccagca ttaaaaccaa accaaccaac
     5281 ccaaagcatt gctgcaccaa ttacaaccat ggtcctatta tgaggaggca tgggtatttt
     5341 tggaaatcct tgtcttggac ccaaaaccaa tgccactatt aaagcaccga ctccacaggt
     5401 cagatgaacc actaaccctc cagcaaaatc aatcacccca gcagtaccta agaagcctcc
     5461 tccccaaacc atatgacagg caggcaaata tacaaaagta atccaaaaca aagagaacaa
     5521 acaaacagct gaaaatttca ttcgctcagc aaaagcacct acaacaagtg ctggagtgat
     5581 gattgcaaag gtcatctgaa acattacaaa caccgtttct gggatagttc ctgaaataga
     5641 atctctcccc actcctgata gaaaaagagc gctgaagtca ccaaacaatt tgccatcacc
     5701 cttgaatgcc aaactatatc cataaattga ccagacaaca gagactagac aggcaattgt
     5761 aaaacactgc attagaacag aaataatatt tttacttctt accaaaccac cataaaaaag
     5821 tgcaagaccc ggtaatgtca tgaacaacac tagtgcagtt gatgtgagaa tccaggaagt
     5881 atctcctgca ctcagctcgt tagcaaagcc aactgaggga ataagaaaaa tcgcaataag
     5941 agtaagtgtt ttagagctca tattctagat tattaaattt tgggtaactt ctaagagaat
     6001 tgtctagctt cttgctgcaa attcttctca ttgacgaata tatataccat tgaacgaagc
     6061 tgtcaagttt aaggtagttt tttagaaaaa aattatctca ataaatccaa ccgaatcttt
     6121 tgaaaatgat cgctaactct ggttttcttt tcgattatct aatctaaatt ataaagcgga
     6181 gtaaaaaaaa ttccgcatta attatgctga tttaatctta agccttaaat caaaatctat
     6241 tgcaccagtc agatcgtggt tttcctttat tgatttgacg ttatgataaa atttatccgg
     6301 catatctgag gttcttctcg a
//
LOCUS       DASCTH010000300         5924 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000762511, whole genome shotgun sequence.
ACCESSION   DASCTH010000300 DASCTH010000000
VERSION     DASCTH010000300.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000762511"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="QGH35_06335"
     CDS             <1..201
                     /locus_tag="QGH35_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009835821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82179.1"
                     /translation="NLNPGIAGLLFMTEIIVGSSTAAILTNEPFGLREITGIVLITLA
                     GLMETIGHSVTRFIRVLFPIEK"
     gene            420..1352
                     /locus_tag="QGH35_06340"
     CDS             420..1352
                     /locus_tag="QGH35_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJL82180.1"
                     /translation="MIKTLTRIIAAVALLVSLASTSIAADSKKPIRIPVHNWSSQVVM
                     AYVIGGIFESIGDNVEYVPADSQAVYESIRQGDVDISHEVWQSAFGASFDAARDAGGL
                     LDWGDHEARTLEDMGYPNWVADLCPGLPDWTALQNPDCAANFVTPDSSGKGRWLEGPQ
                     TWHQDLMPQRLEALGLGDLWTVKFAGGADALWAELAAAKKENRGTIIFNWTPNFTDGA
                     GFTFIDFPPYYDGCRPADGGDGKCGSPDGYLKKAVNENFPKTHPDAAAIFKNMSFSTS
                     HIGAMAALVDVDGMTHEDAAKAWLADNESVWSAF"
     gene            1460..1909
                     /locus_tag="QGH35_06345"
     CDS             1460..1909
                     /locus_tag="QGH35_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82181.1"
                     /translation="MRKIIIYLFLILILSSTASARKTGCEGDCENGIGTWTYTDKTVY
                     VGEWRDTLKHGQGTETWPNGYIYTGEFFKSKWHGQGTLTFPDGATYVGEWRDANMNGQ
                     GTFTWANGDMYAGAFLDSARNGQGTKTSADGTVQKGIWKDGELVEPN"
     gene            1918..2445
                     /locus_tag="QGH35_06350"
     CDS             1918..2445
                     /locus_tag="QGH35_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82182.1"
                     /translation="MLLENNLKKYKETPEGFRKLVGLVGLLAIIIFAFIILNNIIIGE
                     NKIAESDKIVENEETIISSLPSGRLNFYESSDGYQLSSSEYEAVCKNTKIVAQRAMMG
                     ALITNINAQKLYTDNGNKIDKFSVNWDSTSNKCFAQYTIRAIQGTSDTITVSGQAKGF
                     FNTGVDTRVYFIKNF"
     gene            2487..3521
                     /locus_tag="QGH35_06355"
     CDS             2487..3521
                     /locus_tag="QGH35_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037505.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HJL82183.1"
                     /translation="MASGQSVIKCESVYKIFGPNAKEMLENANGNVDAKTFQEAGCIV
                     GVNNASFDVSKGELLVIMGLSGSGKSTLLRCISRLTDATSGKIFIDGQDLLSLKNKEL
                     IELRRNKMGMVFQSFALLPHKTVLENIAFPLQIKGLKTRDSISKAMDMVKLVGLDGRE
                     NYFPRELSGGQQQRVGIARSLAVEPDVWFLDEPFSALDPLIRKEMQDEFLRLQSSLKK
                     TILFITHDFDEALRLADRIGIMKDGIIEQLDTPANIVLNPATEYVRKFTQEVPREKVL
                     KIEAVMDPITDEQNISDFKVSKDAIIETVAEKILGQEKPIAVTDSNQQIVGIIKPAKV
                     IHVVFGEGNE"
     gene            3535..5691
                     /locus_tag="QGH35_06360"
     CDS             3535..5691
                     /locus_tag="QGH35_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJL82184.1"
                     /translation="MEIINKYSKLFQWTFLLVVFFGLCFAIDVPEGYKFIRAQAEHIK
                     VAGQSEYDFFGINVRYIYFNFLWRLPPWLGWLPIWVNDSLHFLLNDWMPIEFWDEDIQ
                     EYKTRPLVLQITRVISGTMLFFIEFIREILIGGVKTVVSFTSWDWIDANPWAELPGLP
                     WTIVTAGAVILGYKLNGKGLAIFAGIAMIYISVFGQWKPSMQTLSFILVAAPVSFILG
                     LSLGIMAYKSKAVERVLYPILLVMQTMPQYAVLVPAMVLFGIGDHSAAIITIVVATPP
                     MILLTLLGLRGVSPEVIEAGKMSGCNNLQLMLRVLIPTARRDILIGVNQVIMVCLSMA
                     VIAAFIGAKGLGWNLLLALNQLNIGLALEAGICISLIAVVLDKMSLAWANKQTDYFGN
                     LTFFQRHKYGMFFAGSVLIGLILAYSGSFYFKEGFNYLYEVPHNKGISTAEFWNGGVD
                     WIWDTFFYPLKIFNTWLIVDVLQPMRAAYLRMPIVATFVLVMGAGYIIGGIRSALVMC
                     GLTLFIALSPWWDRALVTAYMATFGVIVSGIIGITVGTLCAQNNYSSKFILAICDIFQ
                     TFPSFVYLIPVMMLFGVTDTSVLIAVIVYATIPATRYTVEGLRSISPALHDAGAMSGV
                     TNIQRIFRIDFPLAFPHIMLGINQTIVFALFMVIIGAFIGTEDLGQYILKALSDLKGG
                     GKGLILGICVAFIALAFDNLIRTYAEKRKKELGFAN"
     gene            complement(5738..>5924)
                     /locus_tag="QGH35_06365"
     CDS             complement(5738..>5924)
                     /locus_tag="QGH35_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018037507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5,10-methylenetetrahydrofolate reductase"
                     /protein_id="HJL82185.1"
                     /translation="DVPEKIINRLEQAQDAKKESKEICKELINKFKEIKGVKGIHLMG
                     HNKEEEIADIIQEARKS"
BASE COUNT         1840 a          913 c         1212 g         1959 t
ORIGIN      
        1 aatctgaatc cgggtattgc cggattattg tttatgaccg aaatcattgt tggatcgtca
       61 acagcagcca tattgacaaa tgaaccgttc ggcctacgtg aaatcactgg cattgtcctg
      121 attacattgg ctggtttgat ggaaactatc ggacactcag taacacgctt cattcgtgtc
      181 ttgtttccga ttgaaaaata aatttttttt tatattcaaa aactcttaaa tcgcgcaatc
      241 acaaggtttt ctgcacaata gtaactgtat tttatactat atgtgtttca tttaaaaaat
      301 gttgttctgg atttttaata ataaagtata atttcaacat atgaaatttt ttattatttt
      361 tttctcaatt ctgtgattaa actgtttcta taaaatcaaa attcaaccta ggaggaatta
      421 tgattaagac tttaacaaga atcattgctg ctgtagcttt gttagtatct cttgcaagta
      481 cttcaatagc agctgacagc aaaaaaccta taagaattcc ggtgcacaac tggtcaagtc
      541 aagttgtgat ggcttatgtc attggtggaa tcttcgaaag tataggtgac aatgtcgaat
      601 atgttcctgc agactctcaa gcagtatacg aatcaattcg ccaaggtgat gtagatatat
      661 cacacgaagt ttggcaatct gcttttggtg catcttttga tgctgcacgt gatgcaggtg
      721 gtctacttga ttggggtgat catgaagcga gaactctaga agatatgggt tatccaaact
      781 gggtagctga tttatgtcct ggtcttccag attggactgc tctacaaaat cctgattgtg
      841 ctgcaaactt tgtaacacct gattcaagtg gaaaaggacg ttggctagaa ggtccgcaaa
      901 cttggcacca agatttaatg cctcagaggc ttgaagcact tggactaggt gatttatgga
      961 ctgttaaatt cgctggtggt gctgatgcgc tatgggctga actagcagca gctaaaaaag
     1021 aaaatagagg aacgatcatc tttaactgga caccaaactt tacagatggt gctggtttca
     1081 cgtttattga tttccctcca tattatgacg gttgcagacc agcagatggc ggtgatggaa
     1141 aatgtggttc accagatgga tatctgaaaa aagcagtgaa tgaaaacttt ccaaagactc
     1201 atccagatgc agcagctatt ttcaagaata tgtcattttc aacaagtcac attggtgcaa
     1261 tggcagcttt agttgacgtt gatggaatga ctcatgaaga tgcagctaaa gcatggttag
     1321 cagataacga gtcagtatgg agtgcttttt aattcaataa ttatttgaaa aaaagtataa
     1381 ctataaaata ttatctcccc ttgtttcaaa ctaggggaga tttttttttt cttttagtgt
     1441 aatttgttaa ataatgcaga tgagaaaaat aattatatat ttatttctga ttttgatcct
     1501 tagtagtaca gcatcagcgc gtaaaactgg atgtgaaggg gactgtgaaa acggaattgg
     1561 cacttggact tatacagaca aaaccgtata cgtgggagag tggcgtgata ccctaaaaca
     1621 tggacaaggg actgagactt ggccaaacgg atacatatac acgggagagt tttttaaaag
     1681 taaatggcat ggacaaggaa ctttgacttt tccagatgga gccacatacg tgggtgaatg
     1741 gcgtgatgct aatatgaatg gacaaggaac ttttacttgg gctaacggag acatgtacgc
     1801 gggagcgttt cttgatagtg ctagaaatgg acaagggact aagactagtg cagacggaac
     1861 agttcagaaa ggcatctgga aagacggtga attagttgag ccgaattaga tataacaatg
     1921 ttgttagaaa ataatttaaa aaagtacaaa gaaactccgg agggatttag aaaattagta
     1981 ggccttgttg gtttgcttgc aataataatt tttgcgttta tcattttaaa caatattatt
     2041 attggagaga ataagatcgc agagagcgat aagattgtag agaatgaaga aacaattatt
     2101 tcttcacttc caagtggtag attaaatttt tatgaaagta gtgatggtta tcaattatca
     2161 tcgagcgaat atgaagctgt gtgtaaaaac actaaaattg tagcccaacg tgcaatgatg
     2221 ggggcgctca ttactaacat caatgcacag aaattgtata cggataatgg aaataaaatt
     2281 gataaatttt cagtaaattg ggatagcact tcaaacaaat gtttcgctca atatacgata
     2341 agagctatac aaggaacgtc tgatacaatt acagtttcag gtcaagctaa aggttttttt
     2401 aatactggtg tagatactcg agtatatttt ataaaaaatt tttaggattt ttattctatt
     2461 ttgatataga ttattgaggt aattttatgg cttctggaca atcagtaatt aaatgtgaat
     2521 ctgtttataa aatttttggg ccaaatgcta aagaaatgct cgaaaatgct aacggcaatg
     2581 tagatgctaa gacttttcaa gaagccggtt gtattgttgg tgttaataat gcttcctttg
     2641 atgtttcaaa aggagaattg ttggttatca tgggattgtc cggatctgga aaatcaacct
     2701 tacttcgatg catatcacga ctgacagacg ccacctccgg taaaattttt attgatggtc
     2761 aggatttact atctttaaaa aataaagaat taatagagtt aaggcggaat aaaatgggta
     2821 tggtttttca aagttttgca ctattgccac acaaaactgt attagaaaat atcgcttttc
     2881 cattgcaaat taaaggcctg aaaactcgag acagtatctc aaaggcaatg gatatggtta
     2941 aacttgtggg tcttgatggg cgggaaaatt attttccaag agaattatca ggtgggcaac
     3001 aacaaagagt aggtatcgcc agatcgttag cagttgaacc cgatgtttgg tttttagatg
     3061 aacctttttc tgctcttgac ccgttgatta gaaaagaaat gcaagatgaa tttttaagac
     3121 ttcaaagttc attaaaaaaa acaatcttat ttattacgca tgatttcgat gaggcactta
     3181 gactagcaga tcgaattgga attatgaaag atggcataat cgagcaatta gatacacctg
     3241 ctaatattgt gctaaatcca gctacagaat acgttcgtaa atttacacaa gaggttccaa
     3301 gggagaaagt cttaaaaata gaggcagtaa tggatccaat tacagatgaa caaaatataa
     3361 gtgactttaa agtttctaaa gatgcaataa tagaaacagt tgctgaaaaa attttaggtc
     3421 aagaaaaacc tatagcggtt actgactcta atcaacaaat agttggaatt ataaaacccg
     3481 ccaaagtaat tcatgttgtt tttggtgaag gcaatgaatg actattaata attcatggaa
     3541 atcataaata aatactcaaa actatttcaa tggacttttt tattggttgt tttttttgga
     3601 ttgtgttttg ccatagatgt accagaaggc tataaattta taagggcgca agccgaacac
     3661 ataaaagttg caggtcaatc agaatacgac ttttttggta taaacgtcag atatatttac
     3721 tttaattttt tatggagact accgccttgg cttggatggc ttcccatatg ggtaaatgat
     3781 tcattgcatt tcttattaaa tgattggatg cctatagagt tttgggatga agatattcag
     3841 gaatataaaa cacgtccact tgttctacaa attacaagag taatctcagg tactatgctt
     3901 ttttttattg aatttattcg tgaaattttg attggaggag tgaaaactgt tgtttcattt
     3961 acaagttggg attggatcga tgcaaatcca tgggcagaat tgcctggact tccttggact
     4021 atcgttacag cgggagcagt tatattgggc tacaaactga atgggaaagg tcttgcaata
     4081 ttcgcaggca tcgccatgat ttatatttcc gtatttggcc aatggaaacc ttcaatgcaa
     4141 acactctctt ttatactagt agcagcccca gtttctttca ttcttggttt gagtttaggc
     4201 attatggctt acaagagcaa agctgttgag cgagtcttat atcccatact tctagttatg
     4261 cagactatgc cccaatatgc agttcttgtc cctgcgatgg ttctgtttgg cattggcgat
     4321 cattccgcag ctattataac aatagttgtt gctactccac caatgatcct attaactttg
     4381 ttaggactta gaggagtgtc tcctgaagtt attgaagctg gcaaaatgag tggctgtaat
     4441 aatttgcaac ttatgctcag agtattaatt ccaactgcga gaagagacat tttaattgga
     4501 gtgaatcagg tcattatggt gtgtttatct atggcagtta ttgcagcttt tataggtgct
     4561 aaaggattag ggtggaattt attgttagcc ctaaatcaac tgaatattgg attagcatta
     4621 gaagccggta tttgcattag tctgattgca gtagtgttgg ataaaatgtc tttagcttgg
     4681 gcaaataaac aaacagatta ttttggtaac ctgacttttt tccagcgcca taaatatgga
     4741 atgttttttg ccggatcagt attgattgga ttgatacttg cctactctgg atctttctac
     4801 tttaaagaag gttttaatta tttgtatgag gttccacata ataaaggaat atcaactgca
     4861 gaattctgga atggaggtgt tgactggatc tgggatacat ttttttaccc attaaaaata
     4921 tttaatacct ggttaatagt agatgtatta caacctatgc gagctgctta tcttcgtatg
     4981 ccaattgttg ctacatttgt tttagttatg ggtgctggtt atataattgg cggaattcga
     5041 tcagctttag taatgtgtgg attgacatta tttattgctt taagtccgtg gtgggacaga
     5101 gcattagtca cagcgtatat ggcaactttt ggagtaatag tttcaggtat aattgggata
     5161 accgtaggta ctctgtgtgc gcaaaacaat tatagctcaa aatttattct agcgatttgt
     5221 gatatttttc aaacattccc gtcttttgta tatttaattc ctgttatgat gcttttcggt
     5281 gtaacggata catccgtttt aattgctgtt attgtttacg caactattcc cgcaactcgc
     5341 tatactgttg aaggacttcg tagcatttcc ccagcgttac acgatgctgg cgcaatgtcc
     5401 ggagtgacta atatacaaag aatatttaga atcgattttc ctttagcatt cccacatatc
     5461 atgcttggaa ttaatcaaac aattgttttt gcattattta tggtaattat tggggccttt
     5521 atcggaacag aggatctagg acagtatatt ttaaaagctt tatcagacct gaaaggtggg
     5581 ggtaaaggat taattcttgg catttgtgtt gcttttattg cacttgcttt tgataatttg
     5641 attcgaacct atgcggaaaa aagaaaaaaa gaattaggat ttgcaaatta atcatcaacc
     5701 taaaagacta tcatttaaaa cggatagcac taattaatca actctttcta gcttcttgaa
     5761 taatgtctgc gatttcctct tctttgttat gccccatcaa atgaatgcct ttgacacctt
     5821 taatttcttt gaatttattt attaattctt tacagatttc tttactttct tttttagcat
     5881 cttgtgcttg ctctaatctg tttattattt tttctgggac atcc
//
LOCUS       DASCTH010000301         5577 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000764463, whole genome shotgun sequence.
ACCESSION   DASCTH010000301 DASCTH010000000
VERSION     DASCTH010000301.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000764463"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="QGH35_06370"
     CDS             <1..334
                     /locus_tag="QGH35_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82186.1"
                     /translation="ISGKTYSIPVSYLKSTDKELFCLTEKEYIWWRNLIDYNTIQILL
                     EGKLLNANISVESENSNLIAEKIKAMCIHSRIDAAFANVRFKDKAPIEEDIIAASSKT
                     VLLQLSII"
     gene            complement(363..1421)
                     /locus_tag="QGH35_06375"
     CDS             complement(363..1421)
                     /locus_tag="QGH35_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012993.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HJL82187.1"
                     /translation="MIDPYLAVGLQTKVKHVTTRSEVQKNLTHIGNMIDMVTHMCSLE
                     LPVRLIALGEGAIQGFVDEILDMEQTKYAETMAAEIPGWETDFLGDYAERKNTYIIGQ
                     LKEKLPEYPDRFFNTVFIIDDKGKVIYKHHKNIVVFVEHSTTPHDVYDQWIEQHGDTL
                     EAFFPVAKTEIGNIAGTVGVEGSFPETFRAFAMNGAEILYRASLPEPWVSREIYEVQN
                     RARAIDNTCYMISPNTGSLIMPGVGGEAPTVVDGALGGRSAIVDYKGTVLAKNNIVGD
                     TYVASEINIDALRHYRENARFQNWIPYLKTEIYKKLYDQEIWPKNLPPMDHDGAGKIF
                     KGAVKKLIESGIYTSRKK"
     gene            complement(1442..2836)
                     /locus_tag="QGH35_06380"
     CDS             complement(1442..2836)
                     /locus_tag="QGH35_06380"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HJL82188.1"
                     /translation="MRRNFRQIIDFLEQEGELVRIKKKISPNKEMAALMWQLEQQNKA
                     FIFENIENSNMPAVGGLFTSYNRLQLIFKEKAAGDFTKDDAGKMIQDAIQQPIKTIEI
                     SHGPVNEVVLSEDEVNLEQLPIPTFFELDSGAFITAAVGIFRKPEGHLNAGFYRANVL
                     DKNHVIINASSLGDLRKSYNWYRENHKKMAVALVLGAPPSLLMAAAAKSPNEISELEV
                     AGGIVGSGLKMVQCQHSDLLVPAEAEMIIETEVDLNNMLPNTLGEFGDQYGTEEAPLA
                     TVKTITKRKDAMFYTLMAGRAKEHNTLGYLAVYGLVRGVTQKIKANNSSIMAVKIHND
                     TKIGPLMHVVVAIKKESDEQPEKIIEEVFNTNIGLHKLSWMAKRVIIVDEDIDITNME
                     DVEWATWTRVARAEKIHLYSDFNTWEIDRSALPNNTSLRVGIDATKDLNQVDNLVRPV
                     ITGIDDVDLKDYLD"
     gene            3012..4118
                     /locus_tag="QGH35_06385"
     CDS             3012..4118
                     /locus_tag="QGH35_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018275659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HJL82189.1"
                     /translation="MKTKAAVAMEAGKPLIIDNLDLDGPREGEVLVEIKATGICHTDA
                     FTLSGDDPEGIFPAILGHEGAGIVVEIGKGVKSVSNGDHVIPLYTPECRECEFCLNPK
                     TNLCQSIRETQGQGLMPDGTSRFSLNGEQIYHYMGCSTFSNYTVLPEIALAKIREDAP
                     FDKVCYIGCGVTTGIGAVVFDAKVEAGSNVVVFGLGGIGLNVIQGAKMIGANKIIGVD
                     INEGKIELAKKFGMTDFLNPISEPNIVEAIIDLTKGGADYSFECIGNVDTMRQALECC
                     HKGWGESVIIGVAGAGQEISTRPFQLVTGRVWRGTAFGGARGRSDVPIIVDWYMDGKI
                     SIDDLITHTMDLDEINDAFTLMHEGKSIRSVVLY"
     gene            complement(4234..5517)
                     /locus_tag="QGH35_06390"
     CDS             complement(4234..5517)
                     /locus_tag="QGH35_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024750.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HJL82190.1"
                     /translation="MSSLKTSITPELIASLEYKDKPYFVLSSNYPGFRLKVNPQGRIS
                     FITYGRVHFGGNPRTITHGTTKNLTLAEAIEKHLYATQLLERGQDPNLIKKSKNKQFA
                     LSLDLESVAIQFISDKEMKNDFSSEYAKQCRDYLLRNKLKPLLSFNINKISTEIIIEW
                     YRTLEKTPTAANNALKFLSSVFSYAELLNITAKNSNPTAVIKRLSLGYPNNKRDKQID
                     IQLELPKLIYRIWDPLNIAKVNRSTRNAIYLLLLSGLKKSDVLNLKWSQINDNKFIKF
                     KRRGYIQFYPIDSHIQDVIDDMRKILKENKKLVINSQADWVFLSSKKQESQITNIRKS
                     LTVYSQDFNWVIYPESLRKTFAKFADQAGIPRSHYYLLLGIKKPYTVYESTENNVTDL
                     ELKNSLIQIHKLYEKICPMQLPGITEPLRHFMFGK"
BASE COUNT         1641 a          990 c          980 g         1966 t
ORIGIN      
        1 gatttcagga aaaacatact ccataccggt gtcttatctt aaaagcaccg ataaggaatt
       61 attctgttta actgaaaaag aatacatttg gtggagaaat ctaattgact acaacactat
      121 tcaaattcta ttagagggca aattactcaa tgctaatatt agtgttgaat ctgagaacag
      181 taatttgatt gcagaaaaaa taaaagcaat gtgtattcat agcagaattg acgccgcttt
      241 tgcaaatgtc agattcaagg ataaggcacc aattgaagaa gatattattg ctgcttcatc
      301 caaaacagtc ttgctacaat tgtcgatcat ataagatgct cttaataaat tgtttagtgt
      361 tgctattttt tacgtgaagt atatattcca gactcaatga gtttttttac tgctcctttg
      421 aaaattttac ccgctccatc atgatccatt ggaggcaagt tctttggcca aatttcttga
      481 tcatagagtt ttttataaat ttcagttttt aaataaggaa tccaattctg gaatctagca
      541 ttttctctgt agtgcctaag tgcatcaata ttgatttctg acgccacata agtatcccct
      601 acaatattgt tctttgccaa aacagtgccc ttataatcaa caattgcaga tctgccgccc
      661 aaggcaccgt caactactgt tggcgcttct ccccctactc ctggcatgat aagggagccg
      721 gtatttggag aaatcatata gcatgtgttg tcgatagctc ttgctctgtt ttgaacttca
      781 taaatctctc ttgaaaccca tggttcaggt aaagatgctc gatatagtat ttctgcccca
      841 ttcatagcaa atgctctaaa tgtctctgga aatgaccctt caacgcccac agttcctgca
      901 atattcccga tttccgtttt agctacaggg aaaaaagcct ctaaagtgtc tccatgttgt
      961 tctatccatt ggtcataaac atcatgtggt gtggttgaat gctcaacaaa gacaacgata
     1021 tttttgtggt gtttatagat gactttaccc ttgtcatcaa taataaagac agtattaaag
     1081 aatctatcag gatattcggg taatttttct ttcagttgac cgatgatata tgtatttttt
     1141 ctttctgcgt aatctcctag aaaatcagtt tcccaaccag ggatttctgc ggccatggtc
     1201 tcagcgtatt ttgtctgttc catatccaaa atttcatcaa caaaaccttg gattgcgccc
     1261 tctcccaatg caattaacct gactggtaat tctaatgagc acatgtgggt taccatatcg
     1321 atcatattcc caatatgtgt gaggtttttt tgaacctcag atcttgtcgt gacgtgtttg
     1381 acttttgttt gtaaaccaac cgctagatat ggatcaatca tgtcatgttc tccttcatct
     1441 cttaatcaag ataatcttta agatcaacgt catcaatacc tgtaataaca ggcctgacaa
     1501 gattgtcaac ttgatttaaa tccttagtgg catcgatacc aacccgaagg gatgtattgt
     1561 taggcagtgc ggatcggtca atttcccaag tattgaaatc agaatataaa tgaatttttt
     1621 ctgcacgagc tactcttgtc caggttgccc attccacatc ctccatattg gtaatatcaa
     1681 tatcctcatc tacaataatg actctttttg ccatccaaga gagcttatgt aggccaatat
     1741 tagtattaaa tacttcttct atgattttct ctggttgttc atcggattct ttttttatcg
     1801 caacaacgac atgcatcaaa gggccaatct tggtgtcatt gtgtatctta actgccatga
     1861 tacttgagtt atttgccttt attttctgag taacacctct aactaaacca tacacggcta
     1921 aataacctaa tgtattgtgt tcttttgctc gcccagccat aagagtatag aacattgcgt
     1981 cttttctttt ggtaatggtc ttgactgttg ctaggggcgc ttcttctgtc ccatattgat
     2041 caccaaattc acccaaagta ttgggcagca tattatttag atcaacttct gtttcaataa
     2101 tcatctctgc ctcagcgggg accagcaaat cactatgctg acactgaacc attttcagac
     2161 cagatcctac aatacctcct gcaacctcta attcggaaat ctcatttggt gattttgcag
     2221 ctgcagccat caacaacgat ggtggtgccc caagaaccaa agcaacagcc attttcttat
     2281 gattttctct ataccaatta taactttttc tcagatcacc taagctactt gcattgatta
     2341 tgacatggtt tttatctaga acatttgctc tgtagaaacc agcatttaag tgtccttcag
     2401 gttttctaaa tattcctact gcagccgtaa taaaagcacc agaatcaagt tcgaaaaatg
     2461 ttggtatggg tagttgttct agattaacct catcctcact taaaacaacc tcattaactg
     2521 gaccatgtga tatttctatg gtttttatag gttgttggat tgcatcttgg atcattttac
     2581 cagcatcatc cttggtaaaa tcacctgctg ctttttcttt gaagattagt tgtaagcggt
     2641 tgtaagatgt aaataaaccc ccgacagctg gcatatttga attttctata ttttcaaata
     2701 tgaatgcttt attctgttgc tctaattgcc acatcagagc tgccatctct ttatttggag
     2761 agattttctt tttgatccta actaactctc cttcttgttc aagaaagtct ataatttgtc
     2821 taaagtttct tctcatgtat ttttttatta tgaagtaaat tttcaatttt tgatactaat
     2881 gtaccaagaa cttaaatatg tgtgaaatca cttacgcata aggatcaaca gagtgaagct
     2941 cattgcattg atttgcaatt cattcagcta ctgataatct tattttagaa tatataaaaa
     3001 ggaggtttcc aatgaaaact aaagctgctg tagcaatgga ggcaggtaag cctttaatca
     3061 ttgataattt agacttagat ggccccagag agggagaggt cttagttgaa attaaagcta
     3121 ctggcatctg ccacactgat gcatttacat tatctggcga tgaccctgaa ggtatatttc
     3181 ctgcaatttt aggccatgaa ggtgccggga tcgtagtcga gattggcaag ggtgtgaaaa
     3241 gtgtttcaaa tggagaccat gtcatacctt tatatacgcc tgagtgtcgg gaatgtgagt
     3301 tttgtctcaa cccaaaaacc aacttatgtc aatcaatccg agaaacacaa ggacaaggac
     3361 taatgcccga tggaacatca cgtttttcac ttaatggaga acaaatttat cattatatgg
     3421 gttgttctac tttctcaaat tacacagtat tgccagaaat cgctctagct aaaattagag
     3481 aggatgctcc gttcgataaa gtctgttata taggctgtgg agtaactact ggtattggtg
     3541 ctgtcgtctt tgacgcaaaa gttgaggctg gttcaaatgt cgttgtcttt ggtcttgggg
     3601 gtattggtct caatgtcatt caaggtgcaa aaatgatagg tgctaacaaa attataggtg
     3661 tagatatcaa tgagggcaaa attgaacttg caaagaaatt tgggatgact gactttttaa
     3721 atccaatctc tgaaccgaat atagtcgaag ccattattga tctaacgaaa ggcggtgcag
     3781 attactcatt tgaatgtata ggtaatgttg acaccatgag acaagcattg gaatgttgcc
     3841 acaaaggatg gggagaatct gtcattattg gtgtagccgg tgctggtcaa gaaatatcaa
     3901 ctaggccttt tcagcttgtt actggtaggg tctggagagg aaccgcattt ggcggtgcaa
     3961 gaggaagatc tgatgtgcca ataatagttg attggtatat ggatggaaaa ataagtattg
     4021 atgatttaat cacgcatacc atggacttag atgagatcaa tgatgctttt actttaatgc
     4081 atgaggggaa atcaattagg tctgtcgttt tatactaggc atatatctta taattatcat
     4141 ttaatatatt tattatgttc ttttgtatgt atttgtttta tatggatttt atatttttca
     4201 tttatattat atatatatag catacataaa ttactatttt ccaaacatga aatgccttaa
     4261 gggttcagta atgcctggta actgcatggg acagatcttt tcgtataatt tatgtatttg
     4321 tattagagag ttttttagtt ctaaatcagt gacattattc tctgtgcttt cataaacggt
     4381 gtaaggtttc tttattccta acaacaaata gtaatgactt cttggtattc ctgcttgatc
     4441 tgcaaatttt gcaaatgttt tccttaatga ctctggataa ataacccaat taaaatcttg
     4501 tgaatacaca gttagtgact ttctaatgtt tgtaatttgt gactcttgtt tcttagaact
     4561 tagaaaaacc cagtctgctt gactgttgat caccaacttt ttattttctt ttaaaatctt
     4621 cctcatatca tcaatgacat cttgaatgtg tgaatctatg ggataaaact gtatgtaacc
     4681 tcttctttta aatttaataa atttgttatc gtttatttga ctccacttaa gatttaaaac
     4741 atctgatttc ttcaagccag ataacaataa taaatatatt gcgttccttg tagacctatt
     4801 aactttggct atgttcagcg gatcccaaat tctataaatt agttttggta actctaattg
     4861 gatatcaatt tgtttatctc ttttattgtt tggataaccc aaacttaatc gttttattac
     4921 tgcagtagga tttgagtttt ttgctgttat attaagtagt tctgcatagg agaaaactga
     4981 tgataaaaat tttaatgcgt tgttagcggc cgtcggtgtt ttttctaatg ttctatacca
     5041 ctcaattatt atttctgtag aaatcttatt gatattgaaa ctaagaagcg gtttcaactt
     5101 attccttaat agataatccc tacattgttt tgcatattca ctggaaaagt catttttcat
     5161 ttctttatca gaaataaatt gaatggcaac ggattctaga tcaagactaa gtgcaaattg
     5221 tttatttttt gactttttaa tgagattagg atcttgacct cgttctaata gttgagtggc
     5281 atataaatgt ttttcaatag cttcagctaa tgttaaattt ttagttgttc catgggtgat
     5341 agttctaggg ttaccaccaa aatgaactct tccataggta ataaatgaaa ttcttccttg
     5401 gggattcact tttaatcgaa atccagggta gttgcttgat aatacaaaat atggtttatc
     5461 tttatattct aaagaggcaa ttaattctgg tgttatgctt gttttgagtg atgacattta
     5521 tatagcatac gtataaaata caaattatta tttaaatacc catatatatc attatat
//
LOCUS       DASCTH010000302         2086 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000764922, whole genome shotgun sequence.
ACCESSION   DASCTH010000302 DASCTH010000000
VERSION     DASCTH010000302.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000764922"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            123..>2086
                     /locus_tag="QGH35_06395"
     CDS             123..>2086
                     /locus_tag="QGH35_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013727.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HJL82191.1"
                     /translation="MFNKGSILRESIIIALFSFALILLISLITYHSDDPGFSTTGTSR
                     EIANYVGLVGAYFSSFTIAFVGLASYCFPILFFVYGFNLMGNKNQDKSYQPLILIKFV
                     AFMFVLLSTCGLASIHLAIPWMPEESGGIVGSIIASFLLKGLGMIGTTLLLSAIWLAS
                     IPIFIGFSWIYLMRQLIGICKQFVSSIRKIFSAVFHWITEKKRTAKKTTLIKDQNQEK
                     VISQEIKPKKTLKKKTKIIKPIKVENEVKKATQEKQTKPFESSSEDTLPELSLLDPAS
                     DKKTGTSEESIEAMSRLLELKLKDFGIDATVEEVLPGPIVTRYEVSPAPGVKVSQISN
                     LSKDLARSLSVSSVRIVEVIEGKSVIGIEVPNDQRELVVLGEILKSTKFEDLKSPLSI
                     ALGKDISGDPIIADLGEMPHLLIAGTTGSGKSVGINAMVLSLLYKATKNDIRLIMIDP
                     KMLELSIYEGIPHLMCPVVTDMKKAANALRWCVLEMDRRYKLMASLKVRNLKGMNKRI
                     NESIKAGTPITDPLFDPDNPSGSGDSLTAPNLEPLPYIIVIIDELADMMLTVGKKVEA
                     LITRLAAKARAAGIHMIIATQRPSVDVITGLIKANIPSRIAFQVSAKVDSRTILDQMG
                     AESLLGNGDMLYIPPGSSTPIRVHGAFVSD"
BASE COUNT          669 a          372 c          405 g          640 t
ORIGIN      
        1 caatgataat attgcaaatg agtatcgatg atgagtatat aattccatta ttatattagt
       61 tttacctatt aaaaaatctt taaaaaaagg aagcaattat ttctaatatt aatatcaaaa
      121 aattgtttaa taagggctcc attctacgtg aatccatcat cattgcttta ttttcttttg
      181 cacttatttt attgatttca ctgatcacct atcattcaga tgatccaggt tttagtacta
      241 ctggaaccag ccgagaaata gcaaactatg tgggacttgt tggtgcatat ttttctagtt
      301 tcactatcgc atttgttgga ttggctagct attgtttccc catcttattt ttcgtatacg
      361 gttttaactt gatgggtaat aaaaaccaag ataaatccta tcaaccactc attctcataa
      421 agtttgtagc ttttatgttt gtcttgctgt caacctgcgg cttagcaagc atccatcttg
      481 ccattccttg gatgcccgaa gaatctggag gcatagttgg ttcaattatc gcttcatttt
      541 tacttaaagg tcttggaatg atcggtacaa ccttactgtt gtcagcgata tggttggctt
      601 ctatacctat ttttattggt ttttcatgga tttatttgat gcgacagttg atcggaatat
      661 gcaagcaatt cgtttcttct atacgaaaaa tatttagtgc ggtatttcac tggataaccg
      721 agaagaaacg cactgctaaa aagacaactc taatcaaaga ccagaatcaa gagaaagtca
      781 ttagtcagga aattaaaccc aaaaaaactc ttaagaaaaa aacaaaaatt ataaaaccaa
      841 ttaaggttga gaatgaagtt aagaaggcaa cgcaagaaaa acaaacaaaa ccttttgaat
      901 caagttctga ggacacgtta cctgaactga gtctgttaga tccagcttca gataaaaaga
      961 ctggcacttc agaggaatct atagaagcaa tgtcgagact tttagagctt aaattgaaag
     1021 attttggtat tgatgcaact gttgaggaag ttttaccggg tccgattgtc actcgatacg
     1081 aagtcagtcc tgcccctgga gtgaaagtga gtcaaatcag taatttatcc aaggatcttg
     1141 ctcgatcctt atcggtatcc agtgtcagaa ttgttgaggt cattgaaggt aaatcagtta
     1201 ttggaatcga agttcctaat gatcaaagag aattagttgt cttgggagag attcttaagt
     1261 caacaaagtt tgaagactta aaatctcctc tctcgattgc acttggtaaa gatatatctg
     1321 gtgatcctat tattgccgat ttaggggaaa tgccccatct tttgattgct gggacaacag
     1381 gctcaggtaa atctgtggga attaatgcaa tggttttaag cttactatac aaagcgacaa
     1441 aaaatgatat acggctaatc atgatagatc cgaaaatgct ggaattatct atctacgaag
     1501 gtattcccca tttaatgtgt ccagtagtca ctgatatgaa aaaagctgcc aacgctcttc
     1561 gctggtgcgt actcgaaatg gatagaagat ataaactgat ggcaagtcta aaagttagaa
     1621 atctcaaggg catgaacaaa cgtattaatg aatcaataaa agcaggaaca ccaattaccg
     1681 atccattgtt tgatccagac aatccatccg ggtctggaga tagcctaact gcccccaatt
     1741 tagaaccatt gccctacatc atcgtcatta ttgatgagtt ggctgatatg atgttgacag
     1801 ttggcaagaa agtggaagca ctcattacca gattagcagc taaagccagg gctgctggaa
     1861 ttcatatgat catcgcaaca cagcgaccat ctgttgatgt tattactggg ttgatcaaag
     1921 cgaatatacc atcaagaatt gcctttcaag tctctgctaa agttgattca agaaccatac
     1981 tagatcaaat gggagcagaa agtttgttag gcaatggaga tatgttgtat attcctccag
     2041 gctcatcgac accgatcaga gttcatggag cctttgtttc tgacga
//
LOCUS       DASCTH010000303         2643 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000765485, whole genome shotgun sequence.
ACCESSION   DASCTH010000303 DASCTH010000000
VERSION     DASCTH010000303.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000765485"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="QGH35_06400"
     CDS             complement(<1..890)
                     /locus_tag="QGH35_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HJL82192.1"
                     /translation="MKRIIVSFLGLWLLYTNPLYSDENESDWMQYGGHEGGGRYSPLN
                     QINKDNVHDLEVAWTFKTGHLDRVPERLSFLRKLVGFQVTPILLPDDVGGHLVLCTPF
                     NEVIALDPTSGEQRWFYSPEINLMPLAGRFNCKGLSQWRDPEANPDELCAHSLFMATN
                     DKRLIALDGRTGTPCPGFGENGQVDVTPYIKALEPTNQIRSSQLKSPPAVVNGVIIIG
                     GTGNKFKDASSINGAVRGFDARSGEFLWAFDTLVRDQNDPDALPYSVGGANVWTTMSV
                     DSERDLVFVPTASASPNFYGG"
     gene            complement(917..2182)
                     /locus_tag="QGH35_06405"
     CDS             complement(917..2182)
                     /locus_tag="QGH35_06405"
                     /inference="COORDINATES: protein motif:HMM:NF019607.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HJL82193.1"
                     /translation="MKNLFPPLLLLLFLSYSESYWAKPIPPELPDIGSSAMKLPILVD
                     SQNLELEVSEGFNIELFIDGLMAPRWMIVMPNDEILVSQSRTETFSGMPKEAVDSLTK
                     LGIFGPSPNNIIKVTVNDNKKSAKTIIDNLNQPFGMIYFQDHLFVANTDSIMRFDYQK
                     ETLSLLGEKIIELPFGPSNNHWTRNIILNAEKDKILVAVGSGTNVNEEGTDTKDRAAI
                     WEINLDGTDKRLYATGLRNPVGMSYDPTSGKLWATVNERDGLGENVPPDYLTEVIDGE
                     FYGWPFVYFGIYPDPIQSKINPKKVVDAQLNARVPDMALGAHTVPLGLLFHSGKNIDQ
                     KYKNGAFVARRGGVSRSILSGYDVVFIPFKDGYPTGEIETFISGFVADQYRGEIYGRP
                     VGLVEMNDGTILLSDDASGKIWKISYKQN"
     gene            complement(2255..>2643)
                     /locus_tag="QGH35_06410"
     CDS             complement(2255..>2643)
                     /locus_tag="QGH35_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HJL82194.1"
                     /translation="RILEENGVEYLCDYTADDHPFRFNTPNNNLIAVPYTVEINDIPA
                     FLGHSGVGVSSEAFGDMIVDQFDVLYEEGATNARCMPICLHTFHVGQPNKFKHLRRAF
                     EYIASHDDVLLTTGDDINDWFREQYM"
BASE COUNT          799 a          589 c          457 g          798 t
ORIGIN      
        1 cctccataaa aattaggaga agcactggct gtaggaacaa aaaccaggtc tctctcacta
       61 tcgacagaca tggttgtcca tacgttggct ccacccaccg aatacgggag tgcatcaggg
      121 tcgttttgat cacgcactaa agtatcaaag gcccataaaa attctccgct tcttgcatca
      181 aaccctctaa cagcaccatt gatagagctt gcgtctttaa atttgttccc cgtcccgcca
      241 ataataatta ctccattaac aacggcagga ggtgatttga gttggcttga tctgatctga
      301 ttggtgggtt ccaaagcttt gatataagga gtgacgtcaa cctgcccatt ctctccaaaa
      361 ccaggacatg gggttcctgt tctgccatcc aatgcgatca atcgtttatc attggtagcc
      421 ataaaaagac tgtgtgcaca tagttcatca ggattggctt caggatcacg ccattgtgat
      481 aaacctttac aattaaatcg cccagctaat ggcattagat taatctcagg actataaaac
      541 cacctctgct ctccacttgt aggatccaga gcaatgactt cattgaaagg agtacagaga
      601 acgagatgtc cgccaacatc atcaggcaat aggattggtg taacctggaa gccaaccaat
      661 tttctcaaaa aagataatct ctccggtacg cgatctaaat gtccagtttt aaaggtccac
      721 gccacttcaa gatcatgcac attgtcttta ttaatttggt ttagaggtga ataccttccg
      781 ccaccttcgt gtccaccata ctgcatccaa tcgctctcat tttcatcaga gtaaagagga
      841 tttgtataaa ggagccacaa tcctaaaaaa cttacaatta ttcttttcat tgtcatcgat
      901 actggcgttt taaatatcaa ttctgtttat agctaatttt ccatatttta cctgaagcat
      961 catcgctaag taatattgta ccatcattca tctctaccag acctactgga cgaccataga
     1021 tctctccacg gtattgatcg gctacaaagc cagagataaa agtttctatc tcaccggtgg
     1081 gataaccgtc tttaaatggt ataaatacga catcatagcc agataatatc gatctagaca
     1141 cacctcctct tctagcgaca aaagctccat ttttatattt ttgatcaata tttttcccac
     1201 tatgaaacaa taaacccaaa ggaaccgtgt gcgccccaag tgccatatca ggaacccttg
     1261 cattgagctg ggcatctact actttttttg gattgatctt tgattggata ggatcagggt
     1321 agattccaaa atatacgaaa ggccatccat aaaactctcc atcaataact tcagttaagt
     1381 aatcaggagg cacattttct cctaacccat ctctttcatt gaccgtagcc cataatttcc
     1441 ctgaagtagg atcataggac ataccaacag gatttcttaa cccagttgcg taaagtcttt
     1501 tgtccgttcc atcaaggttt atttcccata ttgcagctct gtcttttgta tcggttccct
     1561 cttcattgac gtttgtacca gacccaactg caaccagtat tttatctttt tctgcattga
     1621 gaataatatt tctggtccaa tgattgttag atgggccgaa tggtaattca ataatcttct
     1681 cacccaataa acttagtgtc tctttttggt agtcaaatct catgattgaa tctgtattcg
     1741 ctacaaacaa atgatcttga aaatagatca tcccaaatgg ttgattcaaa ttatcaataa
     1801 tagttttagc actctttttg ttgtcattaa ctgttacctt gatgatattg ttcggtgagg
     1861 ggccaaaaat tcctaacttg gttaaagaat caactgcctc ctttggcatg cctgaaaatg
     1921 tttctgttct tgattgagaa accaaaatct catcattggg cattactatc atccaacgtg
     1981 gtgccattag accatcaata aaaagttcaa tattaaaacc ttccgaaact tccaattcaa
     2041 ggttctgcga atcaaccaat ataggcagct tcatcgcaga ggatccgata tcaggtaatt
     2101 caggaggaat gggtttggcc cagtaacttt ctgaataact aagaaacagt aatagcagca
     2161 gaggggggaa tagatttttc atgctaaata atgacagcga gtagtctgat cactcactcg
     2221 ttaaaatgat cactcaatct cagagctttc taatttacat atactgctct ctaaaccagt
     2281 cattgatatc atcacctgtc gtcagtagaa catcatcatg actagcaata tattcaaaag
     2341 ctcttctcaa atgtttgaat ttattgggtt gaccaacatg gaatgtatgt aaacaaatcg
     2401 gcatacatct agcattggtt gctccttctt cgtagaggac atcaaattga tccacgatca
     2461 tatcaccaaa tgcttctgaa gaaacgccaa cccctgaatg cccaagaaat gcaggaatat
     2521 cgttaatttc aacggtataa ggcactgcaa tcaaattatt atttggtgta ttgaacctga
     2581 aaggatgatc atctgctgtg tagtcacaaa gatactcaac tccattttct tcaagaattc
     2641 ttg
//
LOCUS       DASCTH010000304         5018 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000771830, whole genome shotgun sequence.
ACCESSION   DASCTH010000304 DASCTH010000000
VERSION     DASCTH010000304.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000771830"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="QGH35_06415"
     CDS             complement(<1..484)
                     /locus_tag="QGH35_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011628288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HJL82195.1"
                     /translation="MQLSKYPLITYKETPSDAELVSHQLMLRAGLIKKLASGLFTWMP
                     IGLKVIRKIEHIVREAMDASGAYELSMPAIQPSNLWEETSRWKEYGNLLLKMKDRHER
                     SFCFGPTHEEVITDIVRNELKTYRQLPINYYQIQTKFRDEIRPRFGVMRAREFLMKDA
                     Y"
     gene            740..1474
                     /gene="pssA"
                     /locus_tag="QGH35_06420"
     CDS             740..1474
                     /gene="pssA"
                     /locus_tag="QGH35_06420"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HJL82196.1"
                     /translation="MPNLFTTANLFAGCFSLINTLNGNYQIAVIAIFVAVFLDGVDGK
                     VARLTNAASNFGKDYDSLSDLVSFGVAPALLFYFWGLNNLSTFINVWEKTAWLMSFFY
                     IVSMALRLARFNNQLQFGNNSYFTGLPSPAAATFVAVILWQCEILLLDPKLSFSVLMS
                     SVIITAALMVSKVPFYSFKSAQSMNRIPFANTIMIPLIFILILLEPPLVLTVMASTYV
                     ASGPINILVRFFNKLYIRSQKSSHSE"
     gene            1535..2131
                     /locus_tag="QGH35_06425"
     CDS             1535..2131
                     /locus_tag="QGH35_06425"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HJL82197.1"
                     /translation="MRKQKEVVSEVISSDPNSWEILKKEINTCELCSLHQTRTQTVFG
                     VGDPNADWLFIGEAPGKDEDAQGEPFVGRAGRLLNEMIFSLDLCREEIFIANILKCRP
                     PNNRDPKASEVDQCFSYLEQQVQLIEPKIIIAVGKVAAQNLLGTDQTMSQLRGRVHYF
                     GAIKIPLVVFYHPAYLLRSPSQKPKAWQDLILAKQMIS"
     gene            2136..3395
                     /locus_tag="QGH35_06430"
     CDS             2136..3395
                     /locus_tag="QGH35_06430"
                     /inference="COORDINATES: protein motif:HMM:NF023128.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJL82198.1"
                     /translation="MHYLKRKKVQAWALYDWANSAFSLSVGTAFLPVLFSNYWNDGVA
                     STVTTFRLGLANTLASLLVAISAPIIGAIADRAGLRSQFIVFFAGIGAGATFGLYFIG
                     EGLWQSAIFLYVIATFGFASSNTIYDSQITQVAEPFEYDQVSAYGFALGYIGGALLFT
                     VNVLMINMYEIFGFVNQTEATLFAFPLVSFWWIIFTLPLLYFVKDTSRGKKEKLISTI
                     KGGILSVYETIKKLKATPNILLFLIAYWLYIDGVYTIIKMAADYGLSQGLDVQNVIVA
                     LLITNYIGFPAALFFGYLAQKIGPKNGIIICISVYILVTIGAVFIDQTYEFYLLALMV
                     GMVQGGIQSLSRSFYATLIPTHHASEYFGFFNLMGKFGDIIGPVLMGYISLLFGSQRI
                     GILSILLLFITGLILFLKVSDHNINSE"
     gene            complement(3399..>5018)
                     /locus_tag="QGH35_06435"
     CDS             complement(3399..>5018)
                     /locus_tag="QGH35_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012361.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HJL82199.1"
                     /translation="NWSKTYFEWMNNIQDWCISRQLWWGHRIPAWYDTNNNVYVGRSE
                     KEVRTKYKIEDTIKLSQDKDVLDTWFSSALWPFSTLGWPKKTSELETFYPTTVLVTGF
                     DIIFFWVARMVMMGLKFMDCIPFNEVYIHGLVRDKDGQKMSKSKGNILDPIDLIQGID
                     LNELIQKRTDGLMQPDMAKTITEATKKEFQSGIPEFGTDALRFTFASLATTGRDVRLN
                     LNQIEGYRNFCNKLWNASRFVTMNTAEIKLQDINLDSENMTLADHWIQSKLHELINTI
                     ARHITSYRLDLVARTLYDFVWHDYCDWYLELSKSILNNEKTTQTQKQTTRFNLLITME
                     TTLRCLHPIIPFITEEIWQSFTDNKENQSIMTEPYPEADDFTYDQNIMDQMDWLIGFV
                     SNIRQIRSEMNISPKKKISVLLKETSSIEIERLKETQNYIQALGSVEAISILSDDDQE
                     PVSATALLDGMKILIPLEGLIDIEDEKQRLQKNLAKIDKDIKSSKQRLLNKDFRSKAP
                     QEIIDQLKTRLEHLIRDKTKIEDQFNRLSKG"
BASE COUNT         1423 a          855 c          923 g         1817 t
ORIGIN      
        1 agtaagcatc tttcatgaga aactctctgg ctctcattac gccgaatcta ggtcttattt
       61 catctctaaa cttagtttga atttgatagt aattgattgg cagttggcgg tatgttttta
      121 gttcatttct aacaatgtcc gtaatcactt cttcatgagt tgggccaaaa caaaatgatc
      181 gttcgtgtct atctttcatt tttaaaagaa gattgccata ctctttccat ctgctagttt
      241 cttcccataa gtttgaaggc tgaatggcgg gcatagatag ttcatatgcc cccgatgcat
      301 ccattgcttc cctgacaatg tgttcaattt ttctaattac ttttagacct ataggcatcc
      361 aggtaaacaa gcccgaagcc agtttcttga tcaatcctgc tctcagcatt aattgatgcg
      421 ataccaattc agcatccgag ggcgtttctt tgtaggttat taaagggtat tttgataatt
      481 gcatggatac gatgactata attattttga gttaattata ctattctatt tttctctctg
      541 atagcgactc tgcagatagt ttttattttt tggatgaaag taatcttgat aaagactatg
      601 gtgactaatt tccaagatca gccccaattc tttataatgt ctttttaatt ttactaccaa
      661 aatactttgt gattttcata tgccaataaa aacgaaaaga attaaggcac gcaaacaact
      721 aaggcaagga atttatctgt tgccaaattt gtttacgact gccaatttat ttgcaggctg
      781 tttctcatta attaatacct tgaatggtaa ttatcagatt gctgtaattg ctatttttgt
      841 agctgtattt ttagatggtg tcgatggtaa ggttgctcgt cttacaaatg cagcttcaaa
      901 ttttggtaag gactatgata gcttgtcaga tctggtgtcg tttggtgtag caccggctct
      961 actgttttat ttttggggat taaacaattt atcaactttt attaatgttt gggagaagac
     1021 agcttggctg atgtcttttt tttatatcgt ctccatggct ttaagattgg cacgttttaa
     1081 taatcaactc caatttggaa ataattccta ttttacaggc ttgccttcac cggcagcagc
     1141 cacttttgtc gcagtgatat tgtggcaatg cgagatttta ttattggatc ctaagcttag
     1201 tttttctgtt ttaatgagct ctgtgattat tacagcagct ttgatggttt caaaagttcc
     1261 cttttatagt tttaaaagtg cccaatccat gaaccgaata ccatttgcca atacgattat
     1321 gattccactg atcttcatct taatcttatt ggaaccacca ttggtactga ctgtaatggc
     1381 atcaacttat gtggcatctg gtccaatcaa tatcttagtt agattcttta acaaattata
     1441 tattcgatct caaaaatcta gtcattctga ataagagaat ttagtgttca gtaggaaaaa
     1501 gatacttcaa ttgatagggg ttgatgtatg gcacatgaga aaacaaaaag aagttgtgtc
     1561 agaagttatt tcctcagatc ctaattcatg ggaaatttta aaaaaggaaa taaatacatg
     1621 tgaattgtgt tcattgcatc aaactcgaac ccaaacggta tttggtgttg gcgatccaaa
     1681 tgctgattgg ttatttattg gtgaagcccc aggtaaggat gaagatgcac aaggagaacc
     1741 ttttgtagga agagcaggac gtttattaaa tgagatgatt ttttctttag atttatgtag
     1801 ggaagaaata tttatcgcca acatactcaa atgcagaccc ccaaataata gagatccaaa
     1861 agcttcagaa gtagatcagt gtttttctta tttagaacaa caggttcagt tgattgaacc
     1921 aaaaatcatt attgcggttg gcaaggttgc tgcacagaat ttactcggaa cagaccaaac
     1981 aatgagtcaa ctcagaggga gagttcacta ctttggggca ataaaaattc ctttggttgt
     2041 tttttatcat ccagcttact tactgagaag cccttctcag aaacccaaag cttggcaaga
     2101 tttaattttg gctaaacaga tgatttctta gctttatgca ttatttgaaa cgaaaaaaag
     2161 tacaagcttg ggcattgtat gattgggcaa attctgcctt ctctctaagt gttggtaccg
     2221 catttcttcc tgtcttgttc tcaaattatt ggaatgatgg agtagcctca actgtgacca
     2281 catttagact gggattggcc aatactttgg caagtttact ggtagctata agtgcaccaa
     2341 tcattggtgc aattgcagat agagcagggc ttagaagtca gtttatagtt ttttttgctg
     2401 ggattggcgc aggggcaaca tttggtcttt actttattgg agaaggtttg tggcaatcgg
     2461 ctatatttct ttatgttatt gccacgtttg gctttgcatc aagcaatact atctatgact
     2521 ctcaaatcac tcaagttgca gaaccatttg agtatgatca ggtttctgcc tatgggtttg
     2581 ccttgggata cataggaggt gctcttttat ttaccgtgaa cgttctaatg atcaatatgt
     2641 atgagatatt tggttttgtg aatcaaacag aagcaacctt gttcgctttt cctctcgtct
     2701 cattttggtg gatcattttt acgctccctt tactttactt tgttaaagac acttctaggg
     2761 gcaaaaaaga gaaacttatt tccaccatta aaggtggaat tttaagtgtt tatgaaacaa
     2821 tcaaaaaact aaaagctaca cccaatattt tattattttt aattgcttat tggttataca
     2881 tcgatggtgt ttatacgatt ataaaaatgg cagctgacta tggtctttct caagggttgg
     2941 atgttcagaa cgtaattgtt gctttactga taacaaatta tattggtttt ccagcagcat
     3001 tattttttgg ctatcttgca cagaaaatag gtcctaaaaa tgggattatt atttgcatca
     3061 gtgtttatat tttggtcacc attggagccg tctttataga tcaaacctat gaattttatt
     3121 tattggcttt aatggttggt atggttcaag gtggtattca aagtctaagc cgatcttttt
     3181 atgccacatt gattcctact catcatgctt ctgaatactt tggattcttt aatttaatgg
     3241 gaaaatttgg cgatattata ggccctgttt taatgggtta tatctcttta ttatttggca
     3301 gccaaagaat tggaatactt tccatcttat tgttgtttat cacagggttg atattatttt
     3361 taaaagtgag cgatcacaat atcaattcgg aataattctt aacctttcga taatctatta
     3421 aattgatctt cgatttttgt cttatcccgt attaaatgtt ccaatcttgt tttgagttga
     3481 tcaataattt cttgaggggc tttagatcta aaatccttat tcaaaagtct ttgttttgaa
     3541 gacttaatat ctttgtctat ttttgctaga tttttctgca acctttgttt ttcatcctca
     3601 atgtctatta aaccttccaa gggaataaga attttcatgc catcaagcag agcggttgct
     3661 gataccggtt cttggtcatc gtcacttaag atactaatag cctcgactga acccaatgct
     3721 tgaatataat tttgtgtttc ttttaaacgt tcaatctcaa tggaagaggt ttctttcagc
     3781 aagacagaga ttttcttctt gggtgaaata ttcatttcag atctgatttg tctaatgttt
     3841 gaaacaaatc caatcaacca gtccatctga tccatgatgt tttgatcata tgtaaaatca
     3901 tcagcttcag gatatggctc ggtcatgatg ctttggtttt ctttgttgtc agtgaatgat
     3961 tgccatattt cttctgttat aaaagggatt atagggtgca agcatcttag tgttgtttcc
     4021 atggtgatca aaagattgaa acgagttgtt tgtttttggg tttgtgtagt tttttcatta
     4081 ttgagaatag attttgacag ttctagatac caatcacagt aatcgtgcca aacaaaatca
     4141 tagagagttc tggcaaccaa atctaatcgg taactagtaa tatgtctcgc aatcgtgtta
     4201 attaattcat ggagtttact ttgaatccaa tgatccgcta gagtcatatt ttctgaatcg
     4261 agattaatgt cttgtagttt aatttctgct gtgttcatag ttacaaatcg agatgcattc
     4321 cataatttat tgcagaaatt tctataacct tctatctggt tgagatttaa tcgaacatct
     4381 cttcccgtcg ttgctagtga tgcaaaagta aagcgcaatg catcggttcc aaattcaggg
     4441 atgcctgatt gaaattcttt tttggttgct tcagtgattg ttttggccat atcgggttgc
     4501 atcaacccat ccgttctttt ttgaattagt tcattgagat caatgccttg tattaaatca
     4561 attgggtcta gaatattgcc ttttgattta gacatttttt gaccatcttt atcacgaacc
     4621 aatccatgga tgtacacttc gttaaagggg atgcaatcca taaattttag acccatcatc
     4681 accattcgtg ccacccaaaa gaaaataata tcgaatccag ttactaaaac agttgttggg
     4741 taaaatgttt ctaattcact ggtctttttt ggccaaccaa gtgttgaaaa aggccacaaa
     4801 gcagaggaaa accaagtatc gagaacatcc ttgtcctggc ttagtttaat ggtgtcttca
     4861 attttatatt ttgttcgaac ttctttttcg gatctgccaa cataaacatt gttattagtg
     4921 tcataccaag ctggaatacg atgcccccac cacaattgac gactgataca ccaatcttga
     4981 atattattca tccactcgaa atatgttttt gaccaatt
//
LOCUS       DASCTH010000305         1572 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000786732, whole genome shotgun sequence.
ACCESSION   DASCTH010000305 DASCTH010000000
VERSION     DASCTH010000305.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000786732"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1572
                     /locus_tag="QGH35_06440"
     CDS             <1..>1572
                     /locus_tag="QGH35_06440"
                     /inference="COORDINATES: protein motif:HMM:NF016289.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HJL82200.1"
                     /translation="DGQELSTNKIDEQHLHQHIQILASDEFEGRAPGSEGGEKTKEYL
                     VNAFKEFSLEPINGDYLLDVPLVEVSVKEGSYISILQDDGEHKLQQGAEAVYWTKRVQ
                     EEVAVEESELVFAGYGIVAPEYGWNDYEGIDMRGKTAVLLINDPGFATQDPTLFKGNA
                     MTYYGRWTYKYEEAARQGAEAVIIIHETAPAAYPWQVVETSWQGKQIDLKREDMGLNR
                     VKVEAWITHSVAQSLFEKSGLNFEKLKQQALSSNFKPLVMKGLKLKAELNNKIKFKNS
                     HNVAAVKEGSLRPNEFILMMAHWDHLGTKEGQGGDNIYNGAVDNATGVAGLLELAETL
                     NDYDNERSLLYLAVTAEESGLLGSAYFAEYPPVDLSQIVAGFNFDALQPGGKSKDVII
                     VGYGASQLEDILARKLKKFGKYIRPDPEPEKGFFYRSDHISLSKKGVPTLYADAGFDK
                     VDGGVEAGQRLAERYTREDYHQSSDEYNPNWDLSGIVEQLSITAEIVLDLANSLNWPE
                     WYEGNEFRSIREESR"
BASE COUNT          509 a          265 c          370 g          428 t
ORIGIN      
        1 atgatggcca agaattgtcg acaaataaaa ttgatgagca acacctacat caacacatac
       61 aaatacttgc atccgatgaa tttgaaggga gagctcctgg aagtgaagga ggggagaaaa
      121 caaaagaata tcttgtaaat gctttcaaag agttttctct tgaaccgatc aacggggatt
      181 atttacttga tgttcctttg gttgaagttt cagtcaagga aggatcctat atctccattc
      241 ttcaagatga tggggaacac aaacttcagc aaggagccga agctgtttac tggactaaaa
      301 gagtacagga agaagttgct gttgaggaga gtgaactggt atttgctggg tacggtattg
      361 ttgctccaga atacggatgg aatgattatg agggcattga tatgaggggc aaaactgccg
      421 tgcttctgat taatgatcca gggtttgcaa cacaagatcc taccttattt aaaggcaatg
      481 ctatgactta ctacggcaga tggacttata aatacgaaga agctgctcgg cagggagcag
      541 aagctgtcat cattattcac gaaacagctc cagctgctta tccatggcaa gttgtggaaa
      601 ctagttggca aggaaaacag attgatttaa aacgagaaga catgggattg aatcgagtca
      661 aagttgaagc gtggataaca cattctgtag ctcaaagtct ctttgaaaaa tctggactta
      721 attttgaaaa acttaaacag caagctctga gttctaattt caaaccttta gttatgaaag
      781 gtctcaagct taaagcagaa ctaaataaca agattaagtt taagaattct cataatgtcg
      841 cagcagtaaa agaaggttca ctaagaccca atgaatttat tctcatgatg gctcattggg
      901 atcatttagg tactaaagaa ggacagggag gggacaacat ctataatggt gcagtcgata
      961 atgccactgg cgttgctgga ctcttggagt tagcagaaac attgaatgat tatgataacg
     1021 aaagatcgct tttatactta gcagtcactg cagaagaatc gggtctttta ggatcagcct
     1081 attttgctga atacccacct gtagatttat ctcagattgt tgccggtttt aattttgatg
     1141 cattacaacc aggtggaaag tcaaaagatg tgattattgt gggctatggt gcttcgcaat
     1201 tggaagatat tttggccaga aaactcaaga aatttggtaa atatatacgt ccagatccag
     1261 aacctgaaaa aggatttttc taccgttccg atcacatcag tctgtctaag aaaggagtac
     1321 caacactata tgcggatgcg ggcttcgata aagtggatgg tggtgtcgaa gcaggtcaac
     1381 gcttggctga acgatatacg agggaggact atcaccagtc ttctgatgaa tataatccta
     1441 actgggactt aagtggtatt gttgagcagt taagtatcac tgcagaaata gttctcgacc
     1501 tagccaattc actaaattgg cctgagtggt acgagggtaa tgagttcaga tcaattagag
     1561 aagagtctcg aa
//
LOCUS       DASCTH010000306         2183 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000831638, whole genome shotgun sequence.
ACCESSION   DASCTH010000306 DASCTH010000000
VERSION     DASCTH010000306.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000831638"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            97..1485
                     /gene="dnaB"
                     /locus_tag="QGH35_06445"
     CDS             97..1485
                     /gene="dnaB"
                     /locus_tag="QGH35_06445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HJL82201.1"
                     /translation="MPPSAKPQQKIESLGKLTLPPYSTEAEAALLGGMMLDPEGQAYD
                     KIADIVSIQDFYHPENKRIFEAVQKLTESGKLTDLLTVSDWLDNSGNFDVKPAIQYIS
                     ELVDNTAGTVNIREYGEIIREKALLRELITISNNIGQSAYKPEGKSPSQLVDEAEQKI
                     FEIAERGNRNSTYLELRQMIKPTYDRLYERSQKGGGLSGISSGFRELDKLTAGLQKGE
                     LIIIAGRPSMGKTAFALNIAEHIALSDKNNPVAIFSMEMSAEQLTFRMISSLGMVQGN
                     ALKTGKLTDKDWDRVDGAIQQMKEAPIFIDDTPSLTPVELRARARRIQRERGLSLIVI
                     DYLQLMQVSGSKENRATEISEITRNLKALARELQIPIVALAQLNRSVEQRTDKKPIMS
                     DLRESGSIEQDADLIGFIYRDEVYHKDTDKKGQAFIDIAKQRNGPIGDFILTFLGRYT
                     KFENYIPQIDDY"
     gene            complement(1495..>2183)
                     /locus_tag="QGH35_06450"
     CDS             complement(1495..>2183)
                     /locus_tag="QGH35_06450"
                     /inference="COORDINATES: protein motif:HMM:NF015436.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HJL82202.1"
                     /translation="GNVTVEDIMLPHNEIIGVDLSDSSEVNKKKIEQHLHSQLPVFEG
                     VLDNIQGVLNLTPFLKKIDLDSFNNTIVMKHIKKPYFIPEKTTLSQQLFEFKRKKEKI
                     GFAVDEYGNIQGLVTIEDIFEEIVGDYLEETKKLNNEMRPIKDGEDFLVNAASNVRSL
                     NKMMNWEIPVEEAKTLNGAILEQLGYIPDQGYEITLDRYKIIIVKTKENAIQTVRIKE
                     TDPDYKLKAV"
BASE COUNT          747 a          354 c          396 g          686 t
ORIGIN      
        1 gaggttgcta taaaaaatac tttggattta gaagaagaaa tcgatagtat caatgaggaa
       61 gatcaaaaag aagaaattat cgaagaatta gattaaatgc ccccatctgc taaaccacaa
      121 caaaaaatag aatcattagg aaaattaact ctacctcctt actcaacgga agctgaagca
      181 gcattactag gcgggatgat gcttgatcca gaaggtcaag catatgacaa aattgcagac
      241 atagtatcga ttcaagattt ctaccatcca gaaaataaga gaatatttga agccgttcaa
      301 aagcttacag aatctggaaa actaactgat cttctgactg tctcagattg gctagataat
      361 tctggtaact ttgatgtcaa gcctgctatc caatatatca gtgaattagt tgataatact
      421 gctggcactg taaacataag agaatatggt gagattatca gagaaaaagc actgctgaga
      481 gaattaatta ccattagtaa taacattggc caaagtgcct ataaacctga gggaaaatca
      541 ccctcccaat tagttgatga ggctgagcaa aagatattcg aaattgcaga aagaggaaac
      601 agaaattcta cttatctcga attaagacag atgatcaagc ctacttatga tcggctatat
      661 gaaagatcac aaaaaggagg tggattaagt ggtatctcaa gtggttttag ggaattagac
      721 aagcttacag caggacttca aaagggagaa ttaattatta ttgcaggtcg cccgtcaatg
      781 ggtaaaacag cttttgctct taatatagct gaacacatag cactttctga taagaataac
      841 ccagtagcta tttttagtat ggaaatgtca gcagagcaac ttacttttag gatgatttct
      901 tcacttggaa tggttcaggg aaatgcattg aagactggaa aattaacaga taaagattgg
      961 gatagagtgg atggtgctat acaacaaatg aaagaagcac caatctttat tgatgacacc
     1021 ccctcactaa caccagttga attaagagca agagctagaa gaattcaaag agaaagaggt
     1081 ttatcgttga ttgtcattga ttatttgcaa ttaatgcaag tgtctggaag caaggaaaat
     1141 agagctactg aaatttcaga aatcacaaga aatctaaaag cattagcaag agaacttcaa
     1201 ataccaattg ttgctctagc ccaacttaat cgaagtgttg agcaaagaac agataaaaaa
     1261 cccattatgt cggacttaag agaatctgga tcgattgaac aagatgcaga tcttattggt
     1321 tttatatacc gtgatgaggt ttatcataag gatacggata aaaagggtca ggcattcatc
     1381 gatatagcta aacaaagaaa tggccctata ggggatttca ttctgacttt tttaggaagg
     1441 tataccaagt tcgaaaatta cattcctcaa atagatgatt attaatctct gattttatac
     1501 agctttgagt ttatagtcag ggtcggtttc tttaattcta actgtttgta ttgcgttttc
     1561 ttttgttttt acaatgataa ttttgtatct gtctaatgta atttcataac cttgatcagg
     1621 tatataacca agttgttcta gaatggctcc attcagagtt tttgcttctt caacaggaat
     1681 ttcccagttc atcattttat ttaatgatct gacatttgaa gcagcattaa ctagaaaatc
     1741 ttcaccatcc tttataggac gcatttcatt gtttaatttc ttcgtttctt caaggtaatc
     1801 tccaacgatt tcctcgaaaa tatcttcaat tgtcaccagt ccttgtatat ttccgtattc
     1861 atcaacagca aaaccaattt tttctttttt tcttttaaat tcaaatagtt gttgactcag
     1921 cgttgttttt tctggaataa aatatggttt ttttatgtgt ttcataacta ttgtattatt
     1981 gaatgagtct agatctattt tctttaagaa aggtgtcaga ttcaagactc cttgaatatt
     2041 atctagtaca ccttcaaaaa caggtaactg tgaatggaga tgctgttcta tttttttctt
     2101 atttacctca ctggagtcac ttagatcaac ccctataatt tcattatgtg gaagcataat
     2161 gtcttcaaca gtgacatttc caa
//
LOCUS       DASCTH010000307         3712 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000842537, whole genome shotgun sequence.
ACCESSION   DASCTH010000307 DASCTH010000000
VERSION     DASCTH010000307.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000842537"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="QGH35_06455"
     CDS             <1..347
                     /locus_tag="QGH35_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014717.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HJL82203.1"
                     /translation="IAVLRSLGVDHGGIIRIFLSIGFLLGFLGSVLGIIVGFFVTSQL
                     TEIIQYLESIFNITLYQKEVYFLSELPVRINWMEVMWIGLIAVLLSLISSVIPSYRAS
                     RLDPADILRLHR"
     gene            355..1050
                     /locus_tag="QGH35_06460"
     CDS             355..1050
                     /locus_tag="QGH35_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006870650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJL82204.1"
                     /translation="MVKKILEAENIKKSFFKGQQEVEVLSGVNLGIEHGKTIAIIGSS
                     GTGKSTLLQILGMLDQPSSGNVKVRGEIVSKLSDKKISRLRNHFFGFVFQFHHLINEF
                     NALENTMMPLLVRRDSIKLAREKAKQLLAEIGLADRFYHKPSELSGGECQRVAVARAL
                     IAKPLVVLADEPTGNLDPETAEKVFDGLINLNHSVGSSLIVVTHNHDLANKLDQIVIL
                     ERGTIAEYDNKRE"
     gene            1162..2928
                     /gene="msbA"
                     /locus_tag="QGH35_06465"
     CDS             1162..2928
                     /gene="msbA"
                     /locus_tag="QGH35_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014703860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HJL82205.1"
                     /translation="MSNNKEVNSAYITYLRLLRYASPYWMIFVLGALAMLIYALTDTG
                     FAFLIKTLTDSFADETSQAELNQLKFYLPVAVIVIFFIRGVSGFFSVYNIGWIGRQVI
                     KALRAEVFLKFLYLPTTFLDRKSNAELLSKMTFNIEQVAESTSNIITIMIRDTLTIIA
                     LSIYMVYLSPRLAGVIFIVAPVIALIVRFLSTLFRRYSHRIQESMGNVTHLIKEILQN
                     HRIIKIFNAQSYENDKFETVNESNRKHNMKLFSTKAIGDAVTIFVASLGVAGVVYVAT
                     LDEVKLAMTVGDFSGFITAMVLLMTPLKRLTNVNAMIQRGIAASASVFLLLDEENEID
                     LGEIEAKRLTGKIEFINASFSYEKAQSTATDAVSLIVQSGETLAIVGKSGGGKTTLVN
                     LIPRFYDLTKGTLLIDDVNVKDYTLNSLRENISLVTQEVTLFNDTIYNNIAYGNFTEE
                     KILKAISAANINEFLDQLPHGLHTPVGDQGILLSGGQRQRIAIARALLKDAPILILDE
                     ATSALDTESEKYIQIALEELMKNRTTFVIAHRLSTVEKVDRIVVLAGGRIVESGTHQQ
                     LIDQDGEYALLHRLQFNDEAQS"
     gene            2912..>3712
                     /gene="lpxK"
                     /locus_tag="QGH35_06470"
     CDS             2912..>3712
                     /gene="lpxK"
                     /locus_tag="QGH35_06470"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HJL82206.1"
                     /translation="MKLSRKLESIWYSNEPPNILLKLLALCFKLVFVIRRQLYKLKIF
                     KQIKVKVPVIIVGNITTGGTGKTPVTIWLVGIFQKAGMTAGVISRGYGGTQPRNKPVL
                     VTKNDNAVEYGDEPVYIAQATDALVCVCTDKVKAAKTLISEGAEVIISDDGLQHYHLA
                     RDFEIIIIDSVRGFGNGYFLPAGPLRENPERIQMTDWILLNGEGNNFDESLSKDIQCN
                     RFKLLDATAVNHNGTVNKSISEFSGSEVYLLAGISNPERLLQILEENGI"
BASE COUNT         1172 a          599 c          773 g         1168 t
ORIGIN      
        1 ctattgcagt attaagaagt ttgggggtag atcatggagg gataataaga atctttctct
       61 ccatagggtt tttgttgggt tttttaggtt cggttttagg aatcatagtt gggttttttg
      121 taacttccca gttgactgaa attatccagt atttggagtc gatatttaat ataactttgt
      181 atcagaaaga ggtgtacttc ttaagtgaat taccagttag aattaattgg atggaagtaa
      241 tgtggattgg tcttattgcc gtattgctaa gcttaatttc atcagttatt ccatcctata
      301 gagcctcaag attagatcct gctgatatct tacgtttaca taggtaagta aaatatggtt
      361 aaaaaaatat tagaagcaga gaatataaaa aaatcctttt tcaaaggtca gcaagaggtt
      421 gaggtgttat ccggtgtaaa tcttggcatc gaacatggta aaactattgc aatcattggg
      481 tcatcaggca ctggtaaaag cacattatta caaatcctgg gaatgcttga tcaaccctct
      541 tcaggtaacg tcaaagtgag gggagaaatt gtttctaagt tgtcagataa aaaaatcagc
      601 agactaagaa atcatttttt tggttttgtt tttcaattcc accacttgat caatgaattt
      661 aatgctttag aaaataccat gatgccatta ttggttagaa gagactctat aaaactagca
      721 agagagaagg caaagcagct tttagcagag attggtcttg ccgatcgttt ttatcataag
      781 ccctctgagc tctcaggagg agagtgccaa agagtagcag tcgctagagc attaatcgct
      841 aaaccgctcg ttgttttggc agatgagcct actggtaatc ttgatcctga aacagcagaa
      901 aaagtttttg atggcttgat aaatttaaat cactcagttg gatcgagttt gatagttgtg
      961 actcataatc atgatcttgc caacaagctc gatcaaatag ttattttaga acgaggcaca
     1021 attgcagagt atgacaacaa aagagaatag ataaaatctt tacaaaaaga ctattattct
     1081 tcattttttc aacacaatag gcttttaaga tagattagag aattggcgta taataagtga
     1141 tgtttttttt aggttcagat tttgtcaaat aataaggaag taaatagtgc ttatataaca
     1201 taccttcgat tgttgaggta tgcttcaccc tattggatga tttttgttct tggagctctg
     1261 gcaatgctga tttacgcatt gactgacact ggttttgcct ttctcattaa aactctgacg
     1321 gacagttttg cagatgaaac cagtcaagcc gagctgaatc aactaaaatt ctatttgcca
     1381 gttgccgtga ttgttatttt ttttatcaga ggtgtttcag gttttttctc agtttacaat
     1441 atcggttgga taggtagaca ggttattaaa gctttgagag cagaggtttt tcttaagttt
     1501 ctgtatcttc ccactacttt tttagatcga aaatccaatg ctgaattact ctccaagatg
     1561 acattcaata ttgagcaagt tgctgaatcc acttcaaata ttataaccat aatgatcaga
     1621 gatacactta cgatcatcgc actaagtata tatatggttt atctcagtcc caggttagct
     1681 ggggttattt tcattgttgc accagtcatt gctttgattg ttaggttcct gagcacgctg
     1741 tttcgcagat atagtcatcg aattcaggaa tctatgggga atgtaactca ccttattaaa
     1801 gagatccttc agaatcaccg aattataaag atatttaacg ctcaaagtta tgaaaatgac
     1861 aagtttgaaa cagttaatga aagcaataga aaacataata tgaagctttt ttctaccaaa
     1921 gctattggtg atgcagtgac catttttgtg gcgtcactag gtgttgccgg tgttgtgtac
     1981 gttgctacac ttgacgaggt taagttagca atgacagttg gcgatttcag tggttttatc
     2041 accgctatgg ttttgctcat gaccccactt aaaagactaa ccaatgtcaa tgccatgatt
     2101 caaagaggaa tagccgcatc agcaagcgta tttttattac ttgatgaaga aaatgaaatc
     2161 gatctcggtg agatagaagc caaaagatta actggaaaga tagaatttat aaatgcttct
     2221 ttctcctatg aaaaagctca gagtacggcc actgatgcag tcagtcttat tgttcaatca
     2281 ggtgaaactt tggcaattgt gggtaagtca ggaggcggaa aaacaactct agttaatctg
     2341 ataccacgat tctacgacct gactaaaggc acgcttctaa tcgatgatgt gaatgtgaag
     2401 gactatactt taaattctct gagagagaat atcagcttag taacacaaga agtgactttg
     2461 tttaacgaca ctatttataa caatatcgct tatgggaatt ttactgaaga gaagatactc
     2521 aaagctattt cagcagctaa tataaacgaa tttttagatc agttacctca tggtctacat
     2581 acaccagtag gcgatcaggg gattctactc tcaggaggtc aaagacaaag aatagcaata
     2641 gcgagagcac tattgaaaga tgcaccaata ttgatactcg atgaagcaac ttctgctttg
     2701 gataccgaat ctgaaaaata tattcagatc gctttagagg agttgatgaa gaacaggact
     2761 acttttgtaa ttgcacatcg tttatcaaca gtcgagaaag tggaccgaat tgttgtactg
     2821 gcaggcggta ggattgttga aagtggaaca catcaacaat tgatcgacca agacggtgaa
     2881 tatgcattgc ttcataggtt acagtttaac gatgaagctc agtcgtaaat tagagtccat
     2941 ttggtactcg aacgaaccac caaatatcct tttaaaattg ttggctttat gttttaaatt
     3001 agtatttgtc attagacgac aactctataa gttaaaaatc tttaagcaaa tcaaagtcaa
     3061 ggttcctgtt atcatcgttg gtaatataac cactggaggg acaggcaaaa cacctgtgac
     3121 tatatggctt gttggcattt ttcaaaaggc gggtatgact gctggagtta tttccagggg
     3181 ttatggtggt acccaacctc gaaacaagcc agtgcttgtt acaaagaacg acaatgcggt
     3241 tgaatacggg gatgaacctg tttatattgc acaagcaacg gatgcacttg tttgtgtctg
     3301 taccgacaag gttaaagcgg ctaaaactct aatatctgag ggggctgaag tgattatttc
     3361 agatgatgga ttgcagcatt atcatctagc aagagatttt gaaatcataa tcattgattc
     3421 tgtcagaggt tttggaaatg gttattttct gcctgcaggt ccgttgagag aaaatccaga
     3481 aaggattcaa atgactgatt ggattttgtt aaatggagag ggtaataatt ttgatgaatc
     3541 tctttcaaag gatattcaat gcaatcgctt taaactacta gatgcaacag cagtcaatca
     3601 caatggtact gtcaataaat cgatcagtga attttcagga tcagaggttt atttacttgc
     3661 tggaatcagc aaccctgaga ggttattaca aatcttagaa gagaatggta tc
//
LOCUS       DASCTH010000308         3259 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000845331, whole genome shotgun sequence.
ACCESSION   DASCTH010000308 DASCTH010000000
VERSION     DASCTH010000308.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000845331"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="QGH35_06475"
     CDS             <1..353
                     /locus_tag="QGH35_06475"
                     /inference="COORDINATES: protein motif:HMM:NF039905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82207.1"
                     /translation="KLQVDDQPWQDFDTFNLSICNGHYFGGGMKPCSEAGLTSGKLEA
                     LHIGPITTLEGFRYMKPIMSGREVNHPAIRIFSFSSLQIEGAEVEFETDGEIPGNTPM
                     KITLIPQALRVNIG"
     gene            585..803
                     /locus_tag="QGH35_06480"
     CDS             585..803
                     /locus_tag="QGH35_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82208.1"
                     /translation="MGTENNSYVQFKDIQKSYDGETLVINGLNLDVTCGGILTLLDVK
                     QTMTGLRGPLKAQGSASAKNKFLSLIFK"
     gene            808..1896
                     /locus_tag="QGH35_06485"
     CDS             808..1896
                     /locus_tag="QGH35_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJL82209.1"
                     /translation="MSTNEPLVRFENVQKSYDGEILVVKDFNLDVEQGEFVTMLGPSG
                     SGKTTCLMMLAGFEPATNGNIILNGKPINNVPPHKRGIGMVFQNYALFPHMTVAENLA
                     FPLEVRNISKSEREKKVNNALDMVRLGEFGSRRPAQLSGGQQQRVAVARSLIFDPELV
                     LMDEPLGALDKNLREQMQYEIKHLHERLGLTVMYVTHDQTEALTMSNRIAVFDDGVVQ
                     QCASPSNLYERPENAFVANFIGENNRLMGTVEAINGDHATVKTDSGDIVVATKINVSS
                     VGERTTLSLRPERIFISPDDNKTPNTYSAMVKELIYHGDHIRTRFSVCGNDDFIVKIP
                     NSSDSAYLSQGNEVNIGWMAEDCLALDG"
     gene            1934..3019
                     /locus_tag="QGH35_06490"
     CDS             1934..3019
                     /locus_tag="QGH35_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020024754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HJL82210.1"
                     /translation="MKHVIHKTLIASAISTAMLAGAVQADVTLVSWGGGYSASQQKAY
                     IDTYSSGKVNMISYNGGLGEVRAQVESGNVQWDILDVLPSQARTGCDEGLFEELDRSI
                     FFPAPDGTPMDEDLMVDVPNDCVVPQIWWSYVPFHKADTFSGEQPSTMQDFFDIDKFP
                     GKRGIHTWASAIVEMALYADGVAIADIYDVLSTQAGQDRAFAKLDTIKDHVVFWSSGA
                     KPLDLINSGEVSMSLAYNGRIGGAILNDGADYVTVWDGQVLEEEWLVLVNGAPNRAAA
                     LHFLVHASSTQAQADQAKWINYGPMRPSALPIMEAGEPWFNTGVNIMEHMPNTDEHMK
                     GGILANPDWWADNGQAVNDRYAAWMAQ"
BASE COUNT          958 a          650 c          744 g          907 t
ORIGIN      
        1 ttaagttaca agttgatgat cagccctggc aggattttga tacgtttaat ctctctatct
       61 gcaatggaca ctattttgga ggaggcatga agccctgttc agaagccggc ttaacaagcg
      121 gaaaattaga agcgcttcat attggcccga taacaacact ggaggggttc agatatatga
      181 aaccgattat gtcgggaaga gaggtcaacc accctgcgat ccgtatattc agttttagct
      241 cgctccagat cgaaggggca gaggttgaat ttgagaccga cggtgaaatt cccggcaaca
      301 ctccaatgaa gatcaccctt atacctcaag cgttaagggt taatataggt taaatgaggc
      361 gtaaacaggc caacaggaga cgtcagaacg acattttttc tcccatgagg ttccgataat
      421 ctaatcctgg taattccttg atgaaattct tattaagtaa gcagaaatta ttgagattat
      481 ctggttttga cgttattctt tatttgaaca agtgttctaa acatcagcta tgcaaagctc
      541 ttaatttgca agtctgaata aaactggagg atgatcggac cctaatgggc acagagaaca
      601 atagctacgt ccaattcaag gacatccaaa aaagctatga tggagagaca ctggttatca
      661 atggcctcaa ccttgatgtc acctgtggtg ggatcctgac tcttcttgat gtgaaacaga
      721 caatgacagg gcttcgaggg ccgctgaagg cccagggctc tgcatctgcc aaaaacaaat
      781 tcctatccct tatatttaaa taactatatg agcacaaacg aaccacttgt aagatttgaa
      841 aatgtacaaa agagctatga tggggaaatc cttgttgtaa aggactttaa tcttgacgtt
      901 gagcaaggtg aattcgtcac tatgttgggc ccatccggct ctggcaagac aacctgccta
      961 atgatgcttg caggctttga gcccgcaaca aatggcaaca ttatccttaa cggcaaacca
     1021 atcaataatg tacctccaca taagagaggt ataggaatgg tattccaaaa ctatgcttta
     1081 ttccctcaca tgaccgttgc tgaaaacctc gcattcccat tagaagtaag aaatatcagt
     1141 aaaagcgaga gggagaaaaa ggttaacaac gctctagaca tggttaggct tggtgagttt
     1201 gggagccgca gaccagcaca actctctggt ggacagcagc aacgcgttgc cgttgccaga
     1261 tcgcttatat ttgaccctga acttgtacta atggatgagc cacttggggc actagataaa
     1321 aatctacgtg aacaaatgca gtacgaaatc aagcaccttc atgagagact tggtttgacc
     1381 gtcatgtacg ttacccatga ccaaacagag gcgctaacaa tgtccaacag gattgctgtg
     1441 tttgatgacg gagttgtaca acagtgcgct tctccatcaa acctgtacga acgcccagag
     1501 aacgcctttg tggcaaactt tattggagaa aataatcgct taatgggtac agttgaggcc
     1561 atcaatggtg atcacgctac agtcaagact gactctggag acatcgttgt tgcaaccaaa
     1621 attaatgtaa gctctgttgg tgagcgtact accctatcgc taaggcctga aaggattttt
     1681 atctcgcccg acgataacaa gacacctaac acttattcgg ctatggtaaa agagttgatc
     1741 taccatggtg accatattcg tacacgcttt agtgtgtgtg gaaatgacga ttttatcgtc
     1801 aaaattccta attcatctga tagtgcatat ttgtcacaag gcaatgaggt aaatatagga
     1861 tggatggcgg aggattgtct agctctggat ggatagagcc taggcctaat ttaaaataag
     1921 aggagaaaaa ataatgaagc atgtaataca taaaactctg attgcaagcg ctatttcaac
     1981 agcgatgttg gctggtgcag tgcaggcaga tgtaactttg gtttcttggg gtggtggcta
     2041 ttcagcttca cagcaaaaag cctacattga tacctattcc tcaggaaagg taaatatgat
     2101 tagttataac ggtggtctag gtgaggttcg cgctcaagtt gaatcaggta atgttcaatg
     2161 ggatatcctt gacgtacttc catctcaagc tcgaacaggt tgtgatgaag gattatttga
     2221 agagctagac agaagcatct tcttccctgc acctgatggc acaccgatgg atgaggacct
     2281 aatggttgat gtaccaaatg attgtgtagt accgcaaatt tggtggtcat atgttccttt
     2341 ccataaggct gacacctttt cgggtgagca accatccact atgcaagact tctttgatat
     2401 agataaattc ccaggaaagc gtggtattca cacttgggct agcgctatag ttgagatggc
     2461 attgtatgct gatggtgttg ctattgctga tatttatgat gtcttaagta ctcaagcagg
     2521 acaggatcgt gcttttgcta aacttgatac aatcaaagac cacgtcgtat tctggtcttc
     2581 aggtgcaaaa ccactagatc taatcaattc tggtgaggtt tctatgtcac tagcctacaa
     2641 cggtcgtatc ggtggtgcta ttctaaatga cggtgctgac tatgttactg tctgggatgg
     2701 acaggttctt gaagaggaat ggttggttct tgtgaatgga gctccaaaca gagctgcagc
     2761 gcttcacttc ttagttcatg cttcaagtac gcaggcgcag gctgatcaag ctaaatggat
     2821 caactatggt cctatgcgtc catctgctct tccaattatg gaagcaggcg agccatggtt
     2881 caatactggt gtgaatatca tggaacatat gcctaatacg gatgagcata tgaaaggtgg
     2941 tatccttgct aatccagact ggtgggcaga taatggtcaa gcggtaaatg accgttatgc
     3001 agcttggatg gcccaataaa cgagtttaat taggttttat actcgattaa taaaaacagg
     3061 tcggtataac cactggcctg tttttatttt ttaccctaac ttaaattttt tgtaaaaaat
     3121 attgctattt ttatgagtga tacttccact atagattctc agggtcaatt actgacgaat
     3181 gatggtgtcc cccttaaaga cagcttaaga aaatgtctac gccgcactaa aattcgctcg
     3241 ttccttttac ttttagcac
//
LOCUS       DASCTH010000309         2912 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000878385, whole genome shotgun sequence.
ACCESSION   DASCTH010000309 DASCTH010000000
VERSION     DASCTH010000309.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000878385"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="QGH35_06495"
     CDS             <1..371
                     /locus_tag="QGH35_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL82211.1"
                     /translation="NYNAHTHSLPRLRLNSFFGLTYNEIRYSINARYLDGYKNLRPLP
                     AAAISSGYENKVDSSLVFDIGASKTLEINNNELKLGLHLMNAFDESAPLLYDSPDFSF
                     DTRLHDPRGRLINLSLDYKF"
     gene            complement(374..1402)
                     /locus_tag="QGH35_06500"
     CDS             complement(374..1402)
                     /locus_tag="QGH35_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012517.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HJL82212.1"
                     /translation="MTEFGVVMEPVPRCSAEMAARIEQMGFNSALCPDTQNLSADPYG
                     QLSLMANQTKTLKIGTGVTHPVTREAAVTASAMATLQEESGGRVICGIGRGDSSAAHI
                     GKKQATTEELRDYVLRLKSYFNGEAVDVGTMQSPIRWIDPKIIQPVPIDIACTGPKTI
                     AMACEVSDQISFAVGSAKERLRWAIKTAEEHLERIGRSRESIKIGAYINLICDYDEKK
                     AISLARMLAGMVAHFTAMKQAPTEHLPKQLKPITEKLQTEYDMKNHSSEKGEHLELID
                     DDFIEWFSIAGPPQKCIDRLGELIEMGLDYIYLLGGSPVPEPRDLRLKAMVDQTEIFA
                     KEVLPIFK"
     gene            1519..2079
                     /locus_tag="QGH35_06505"
     CDS             1519..2079
                     /locus_tag="QGH35_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024865.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HJL82213.1"
                     /translation="MDDLQIEIENVFRDAEKIWDSQQYGNLKNLWDQEDPSPFYLAEE
                     QSNWKIGWEALKKYWEPIPGKRMIEAIRMRFYDIQVKRLSDDLVFAAGWIRHDMKIRG
                     PMKAWGGDARVSAVLRKKEDGWKFITYAESHKTPLIYMKELYQKQPRIPVITPIVERF
                     MTRLYEKNVHPEFSEFHKQIMENEKT"
     gene            2066..2878
                     /locus_tag="QGH35_06510"
     CDS             2066..2878
                     /locus_tag="QGH35_06510"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJL82214.1"
                     /translation="MKKPDHQASQREKRNSSITKLTDPFAQTKGITNIPKPLFPGLIP
                     TLNKRGFMEEDLNDISRRFVEESNGLDGLSLDMGCAYGVATLAALQNGLHVLASDMDQ
                     AHLDILIKETPKEDLPRLKTKKGTLPEINFKDEMFTTIHCSRTLHFLMPDDLKTTLTK
                     MYRWLRPKGKVYFVTDTCFSGPWQKFLPEFKKRKAAGELFPGLIEDVLQCLPIPELPS
                     GMTPYMNCLDPETLARECRDAGFKILESTFLGFSHNEDKDAKDHAGVIAIKN"
BASE COUNT          912 a          593 c          534 g          873 t
ORIGIN      
        1 ttaattataa tgcacatacg cactcacttc ctcgacttag attaaactca ttctttggct
       61 taacctataa tgaaattcgt tattcgatta atgctagata tctggatggc tataagaatc
      121 taagaccttt acctgcagcc gctatttcta gtggttatga aaataaagtc gattcttcgt
      181 tggtatttga tattggagca agtaaaactc ttgaaataaa taacaatgaa ctaaaactag
      241 gactacattt aatgaatgct tttgatgaat cggcaccact gctttatgat tcaccagact
      301 ttagctttga tacgaggcta catgaccccc gaggtcgact catcaacctt tccttagact
      361 ataaatttta gctttattta aatattggca gaacttcttt agcaaagatc tctgtctgat
      421 caaccatagc ttttaaacga agatctctcg gttctgggac aggggatcct cccaataaat
      481 aaatataatc caaacccatt tctatcagtt cacccaatcg atcaatacac ttttgaggag
      541 gaccagcgat tgaaaaccat tcaataaaat catcatcaat cagctcaaga tgctcccctt
      601 tttcgctaga atggtttttc atatcgtatt cagtttgtag tttttcagta attggtttta
      661 actgcttagg taaatgttct gttggtgctt gtttcatggc agtaaagtga gctaccatcc
      721 ctgctaacat tcttgccaaa gaaattgctt tcttttcatc ataatcgcat attaaattta
      781 tataagcccc aatcttgatg ctctctcgag acctccctat tctctcaaga tgctcttctg
      841 ctgttttaat ggcccaacga agtctttctt tagcactccc tactgcgaat gagatttgat
      901 ccgaaacttc acaagccata gcaattgtct tgggtccagt gcatgcaata tcaataggca
      961 ctggctgaat aatcttaggg tcaatccaac gaatcggtga ttgcattgtg cccacatcga
     1021 ctgcttcacc attaaaatag gacttcagtc ttaatacata gtctctcaat tcctcagttg
     1081 ttgcttgctt cttgcctatg tgggctgccg aagaatcacc ccgacctata ccacagataa
     1141 ctcttccacc tgattcttct tgtagtgtag ccattgctga agctgtgact gctgcttcac
     1201 gagtaactgg atgtgtgacc cctgttccta ttttcaaagt tttagtttgg ttggccatta
     1261 atgacagttg accataagga tcagcagaga ggttttgggt atctggacac agtgcagagt
     1321 taaatcccat ttgctcaata cgtgcagcca tttctgcaga acatcttgga actggttcca
     1381 tgacaacacc aaactcagtc aatttttttc tccattttta tctgtatgat cattatatct
     1441 taactgcaac tgctagctat tttctatagc caaaaccaaa caatccatgc aaaatgaatt
     1501 cagtttaagg aatattacat ggatgattta caaattgaga ttgaaaatgt ttttcgtgat
     1561 gcggaaaaga tttgggactc acaacaatac ggcaacctca aaaacctctg ggatcaagaa
     1621 gatccctctc ctttttattt agctgaagaa caatcgaact ggaagatcgg ttgggaagca
     1681 ttaaagaaat attgggaacc aataccaggc aaaaggatga tagaagcaat ccgaatgaga
     1741 ttctatgata ttcaagttaa acggttatct gacgatctgg tttttgctgc tgggtggata
     1801 aggcacgata tgaaaatacg gggtccaatg aaggcttggg gcggggatgc gagagtctct
     1861 gctgtcctga gaaagaaaga ggatggttgg aaatttatta cttatgcaga aagtcacaaa
     1921 acacccctca tttatatgaa agaactctac cagaaacaac caagaatccc tgtcatcaca
     1981 ccaattgttg agcgtttcat gacacgttta tatgagaaaa atgtacaccc agagttttca
     2041 gaatttcata aacaaataat ggaaaatgaa aaaacctgac catcaagcta gtcaaagaga
     2101 aaagcgtaat tctagcatta caaaattaac tgatcctttt gcacaaacaa aaggtataac
     2161 aaacattcct aaaccactct ttcctggtct aattccaacg ctgaataaga gaggcttcat
     2221 ggaagaagat ctcaatgaca tatcacgaag atttgtggaa gaatccaatg gactggatgg
     2281 attatctcta gacatgggct gtgcttacgg agtagctaca ctggcagcac tgcaaaatgg
     2341 attgcacgta ttggcaagcg acatggatca agcacacctg gatattctta tcaaggaaac
     2401 tcctaaagaa gatctaccca gactaaaaac taaaaaagga acattgccag aaattaattt
     2461 taaagacgaa atgttcacaa ctattcattg ttcccgaact ttgcattttt taatgcctga
     2521 tgatcttaaa acgaccttga caaaaatgta tagatggctc agacctaagg gaaaggtata
     2581 ttttgttacc gatacttgtt tttcaggtcc atggcaaaaa tttctaccag aatttaaaaa
     2641 aagaaaagca gcgggtgagc tcttccctgg acttatagag gatgtcttgc aatgtctacc
     2701 tatcccagaa ttaccctcag ggatgactcc ttacatgaat tgcctggatc ctgaaacgct
     2761 agcccgtgaa tgtagagatg ctggatttaa aattcttgaa tcaacctttc taggtttttc
     2821 tcataatgag gacaaagatg ctaaagacca tgccggtgtc attgccatta aaaattagat
     2881 ctccctcaaa ttgctatatt ctttgatagt gt
//
LOCUS       DASCTH010000310         1585 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000925846, whole genome shotgun sequence.
ACCESSION   DASCTH010000310 DASCTH010000000
VERSION     DASCTH010000310.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000925846"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="QGH35_06515"
     CDS             <1..970
                     /locus_tag="QGH35_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012424.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HJL82215.1"
                     /translation="LTETQFEGVEQDTDFINKSFKGVHASPSVRKLARELGVDLTLIT
                     GTGMKGRVKDEDLKVYVKSILSSKRSHQQASIPEVPEIDYGSFGEIEVVPLSRIKKIS
                     GPRLHASWLNIPHVTQHDEADINEIEQIRLNTKKEAAQQGIRLTLLSYIIKSVCKVLM
                     EFPEFNSSIKPNEDSLTIKKYINIGFATDTPQGLLVPVIKNADQKDILTIATELSELS
                     ESAREKKIKLEDLQGGTFTISSLGGFGGTGFTPIINAPEVAILGVSRAQVKPVFLNGQ
                     FLPRLILPFSLSYDHRVADGVAGIRFTSRLKALLEDPETLLDSMDE"
     gene            963..>1585
                     /locus_tag="QGH35_06520"
     CDS             963..>1585
                     /locus_tag="QGH35_06520"
                     /inference="COORDINATES: protein motif:HMM:NF019604.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HJL82216.1"
                     /translation="MSKTVKITVPNLGDFETVEIVEVHTEAGQSINIDDPLITLETEK
                     AAMDVPCTVNGILESISVSLGDQISEGDVIASVKVDMSSDEDESSGQSQSIKNDQNQT
                     GLVVIGAGPGGYTAAFRASDLGLDVTLIEKHTNLGGVCLNVGCIPSKTLLHAAKIIND
                     SKEAKTLGLNFGEPEIELEKMKDWKNQVISGLTQGLQGLSKKRNINV"
BASE COUNT          516 a          285 c          358 g          426 t
ORIGIN      
        1 gttgactgaa actcaatttg agggtgtaga acaggatact gatttcataa acaagtcatt
       61 taaaggggta catgcgagcc cttctgtaag aaagttggca cgagaattag gcgttgattt
      121 gaccctgata acaggtacag gaatgaaagg gcgtgttaaa gatgaagatc ttaaggttta
      181 tgtgaaatcc attctttctt caaagaggtc acatcaacag gcgtcaattc cagaagtgcc
      241 agagattgat tatggaagct ttggagaaat cgaagtggtt ccactcagcc ggattaagaa
      301 aatttcagga ccaagactcc acgccagttg gttgaatata ccccatgtca ctcaacacga
      361 tgaggcagac attaatgaaa ttgaacagat tcgtttgaat actaaaaaag aagcagccca
      421 acaggggatt cgtttaactc tgctttctta catcattaaa tcagtatgca aagtgctgat
      481 ggaattccct gaatttaatt cgtctataaa acccaacgaa gacagtttaa ccattaaaaa
      541 atacattaat attggttttg caacagacac cccacaaggc ctattggtcc cggtgattaa
      601 gaacgcagac cagaaagaca ttcttaccat tgcaacagaa ctgagtgaac tcagcgagag
      661 tgcaagggag aaaaaaataa agctggaaga tctgcaaggt gggactttca ccatttcaag
      721 cctgggcggt tttggcggta ctggatttac gccaatcatc aatgctcctg aagtcgctat
      781 tttaggtgta tctagagcac aagtgaaacc ggtgttctta aatggtcaat tcctgcccag
      841 attaatattg ccattttctt tatcctatga tcatagggtc gcagatgggg ttgccggtat
      901 ccgatttact tctcggctca aagcattatt agaagatcca gaaactctct tggattccat
      961 ggatgagtaa aacagtcaag ataacagtac ccaatttggg ggattttgaa acagtagaga
     1021 ttgttgaagt tcatactgag gctggacaga gcatcaatat tgatgatcct ctaattaccc
     1081 ttgaaaccga aaaagctgct atggatgttc cctgcactgt aaatgggata cttgaatcca
     1141 tctcagtttc actcggtgat cagatttcag aaggagacgt gattgcttct gtgaaggttg
     1201 acatgagcag tgatgaggat gaaagttctg gtcaatctca gtcaataaaa aatgatcaaa
     1261 atcaaacggg attagtggtt ataggtgctg gaccaggtgg ttatacagca gcattcagag
     1321 cgtctgattt aggcctggat gttacgctta tagaaaaaca caccaatctt ggtggagtgt
     1381 gcctaaatgt gggctgcatc ccatcaaaaa cattgcttca tgctgcaaaa attattaacg
     1441 attcgaagga agcaaaaact ctaggattga attttggaga gcctgaaatt gaattagaaa
     1501 aaatgaaaga ttggaagaac caagtaattt ctggtttaac acaaggattg cagggtttgt
     1561 ccaagaagag aaatatcaat gtcat
//
LOCUS       DASCTH010000311         1534 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000927747, whole genome shotgun sequence.
ACCESSION   DASCTH010000311 DASCTH010000000
VERSION     DASCTH010000311.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000927747"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="murJ"
                     /locus_tag="QGH35_06525"
     CDS             <1..1263
                     /gene="murJ"
                     /locus_tag="QGH35_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HJL82217.1"
                     /translation="AAPILILIFAPGFFNDQAMESVNRYDLAVAMLRFTFPYLLFISL
                     AAFAGAILNTSKKFWATAFTPVILNVVLIIASGWLAPNASSPGMVLAMAVFVAGLLQM
                     VFLLPFLKHIKQLPKPKWGWRDSGVRRVIKLMIPSIIGSSAAQMNLLFNTLIASFLAS
                     GSISWIYYSDRLLEFPVGVFGVALSTVVLPSLSAKRAARNEDQFKSTVQWGVRMSLLL
                     SIPAATGLCVLSGPLVATIFLGGNFNTQDLYMTQYSLMAYAYGLIGLMLVLILASAFY
                     ARQNTKTPVKFGLVTMATNAILSIIFVSVLLSKDISYPHIGLSLAFSCSTLLNAFLLF
                     RALYLEGIIKLDWDVIVFVIRIITATAVMAIFLIVMNPDLSIWIDQALGQRVKHLLLL
                     IPSAVVIYSIVLFCLGLRLEGIRVQTNR"
     gene            1297..>1534
                     /locus_tag="QGH35_06530"
     CDS             1297..>1534
                     /locus_tag="QGH35_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HJL82218.1"
                     /translation="MKDYKDTLNLPDTGFPMKANLPNREPPILERWFKGDLYQKIRKQ
                     RKGSNSFLLLDGPPYANGKIHIGHVVNKVLKDIVI"
BASE COUNT          387 a          310 c          320 g          517 t
ORIGIN      
        1 gcagcaccca ttttaatact catttttgcc cccggttttt tcaatgatca ggccatggaa
       61 tcggtgaatc gatatgacct agctgttgcc atgctccggt ttactttccc gtatcttcta
      121 tttatttctc tggccgcatt tgcaggtgct attttaaata ccagcaaaaa attttgggct
      181 acagcattca cgccagtcat cctgaatgtg gttctgatta ttgcttccgg ttggttggcc
      241 ccgaatgcaa gcagtcctgg catggtattg gcaatggcag tctttgttgc agggttgttg
      301 caaatggttt ttttattgcc atttttaaaa cacatcaagc agttacccaa acccaaatgg
      361 ggatggcgtg attctggtgt tagaagagtg attaaattaa tgattccttc aatcatagga
      421 agctcagccg cgcagatgaa tttgctgttt aatactttga tcgcttcttt tttggcatca
      481 ggcagtatca gctggatcta ttattcagat cgcttgcttg agttcccggt tggtgttttt
      541 ggggttgccc tgtcaacagt ggtgttgcca agtttatcag ccaaacgtgc tgcccggaat
      601 gaggaccaat ttaaatccac agtgcaatgg ggtgttcgca tgtcattgtt gctttctatc
      661 ccggctgcaa caggactttg tgttctttca ggacccttgg tcgcaactat atttttggga
      721 ggtaatttta atacccaaga tctttatatg acccaataca gtctcatggc ttatgcttat
      781 ggattgattg gattgatgtt ggtactgatt cttgcttcag ctttttatgc cagacaaaac
      841 accaaaaccc cggtaaaatt tggactagtt accatggcga ctaatgcaat actctccatt
      901 atctttgttt ctgtcttatt gagtaaagac atttcatatc ctcatattgg tctatcgcta
      961 gctttttcat gctccacttt gttgaatgcg tttttattat tcagagcttt gtatctggag
     1021 ggaataatca aactcgattg ggatgtcatt gtttttgtta ttcggatcat caccgcgact
     1081 gcagtgatgg cgatttttct aattgttatg aacccggatc tcagcatttg gattgatcaa
     1141 gccttaggcc aacgtgtcaa acatttgctg ttgctgatac cttcagcggt tgttatttat
     1201 agcatagtat tgttttgctt aggtcttaga ctagaaggca tccgcgtgca aactaatcgt
     1261 taacctttat aatggttcca tagaaattct atcccaatga aagactacaa agatacactg
     1321 aatttgcctg atactgggtt ccccatgaag gccaatttgc caaaccgtga accaccgatc
     1381 cttgaacggt ggtttaaagg tgatttatat caaaaaataa gaaaacaacg aaaaggcagc
     1441 aacagttttt tactccttga tggcccccct tatgcgaatg gaaaaatcca tattgggcat
     1501 gtagttaaca aagttctgaa agatattgtc atta
//
LOCUS       DASCTH010000312         3540 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000935229, whole genome shotgun sequence.
ACCESSION   DASCTH010000312 DASCTH010000000
VERSION     DASCTH010000312.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000935229"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="QGH35_06535"
     CDS             complement(<1..372)
                     /locus_tag="QGH35_06535"
                     /inference="COORDINATES: protein motif:HMM:NF022072.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HJL82219.1"
                     /translation="MKTIQRSIEIQAAHHNIYNIVADIEQYPIFLPWCESSEIIQNHS
                     NIVIAKLSLNLFGFRTSITTENQMQKPENIKMTLKSGPFRKFNASWLFEPKSASTTQV
                     TYTMNYQLINPLVEKIIEKNID"
     gene            complement(376..1752)
                     /locus_tag="QGH35_06540"
     CDS             complement(376..1752)
                     /locus_tag="QGH35_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HJL82220.1"
                     /translation="MTNTDSTILRQSLHGQWSSRIVFILAVSGSAVGLGNIWRFPYMA
                     GINGGGAYVLIYLLCIFAIGLPIMISEILLGRRGRRNPMTSMELLGLEETGIKSWKYA
                     GVIGLLAGFLILSYYSVIAGWTLNYFILTAQGVFDGIGPSGVDEIFNALTLSIKTQVI
                     FHTIFMVLTILIIAKGIKAGLERAVTIMMPALFLIMLVLIIYAITQGNFLEGLSFMFR
                     PDFTKLDGSSLLSAMGQAFFTLSLGMGCVFTYGAYLPKEESIFKSTIAIIFCDTLIAL
                     LAGMIIFPIVFSYGLEPAEGPGLIFKTLPLAFGQISGGALFGSLFFILLGFAALTSAI
                     SLLEPSVVWMIEQKNVSRKVSAITIGIGIWALGLLTVLSFNELSDFTFWKGTVFDNFE
                     HLASNILLPTSGLLFTVFASWFMSKKSSSEELEGEHSWQYKFWRFLARYIAPIAVFII
                     FLNVIGIF"
     gene            1810..2268
                     /gene="smpB"
                     /locus_tag="QGH35_06545"
     CDS             1810..2268
                     /gene="smpB"
                     /locus_tag="QGH35_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HJL82221.1"
                     /translation="MTRIIVENKKANFNFFIEEKIEAGISLKGWEVKSLRSNRGNIKE
                     SYALVKNGEIWLIGSHISPLPNVSVDDEKDATRTRKLLLKSREISKINGFITQKGYTL
                     IPLSLYWKSGRVKVSIGLAKGRKKHDKRALLKERAWKLDQKRLIKKNYNN"
     gene            2270..3346
                     /locus_tag="QGH35_06550"
     CDS             2270..3346
                     /locus_tag="QGH35_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82222.1"
                     /translation="MKQTMIYITLWSLSLLKSPAAISIENYHHGKHQTIATINCPQKQ
                     DTEGRKYLSMTMMHTNETFNELRSNVHVFNKLSNEQIMMAMKSMGPNYYWTLDKQDPD
                     QTTGALILAHGYGKDGDRSFRNSMEPLSSKYITSLSLGMSMMTSKHIECALLDMQQKG
                     AENIYVVPISSSSRNSLIQQWEYIFGLRNDHAYTKVRSVQMDNVVFLNPIDDHLIAKQ
                     IIFDFAQEISMNPGNEVVIIIAHGPVSKTNNEFELELLENIAIYIREYGKFNDVRAFT
                     IQDDAPKEIRNTNINQIRECIEEASLNGNSVLMVSNLMSGKGIQKKIYDDFSGLDYKF
                     NPKGFLNHPRFNEWILQSIESNHN"
     gene            3405..>3540
                     /gene="ssrA"
                     /locus_tag="QGH35_06555"
     tmRNA           3405..>3540
                     /gene="ssrA"
                     /locus_tag="QGH35_06555"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1279 a          628 c          601 g         1032 t
ORIGIN      
        1 atcaatattt ttctcaatta ttttttctac taacggatta atcagttgat aattcatagt
       61 gtatgttact tgagttgttg atgctgattt tggctcaaac aaccaactgg cattaaattt
      121 tctgaacggt cctgacttta gggtcatttt aatattttcc ggtttctgca tttgattttc
      181 agttgtaata gatgtacgaa acccaaataa attcaaactt agttttgcaa taacaatatt
      241 gctatgattc tggataatct ctgatgattc acaccagggt aaaaaaatag gatattgttc
      301 aatatcagca acaatattat aaatgttgtg atgtgcggct tgtatctcga ttgaacgttg
      361 gattgttttc aaattttaga agatcccaat tacattaagg aaaattataa acaccgcaat
      421 tggggctatg tatcttgcca gaaatctcca aaacttatat tgccatgaat gttccccttc
      481 tagctcttct gaggatgatt ttttagacat aaaccaactt gcaaaaactg taaaaagcaa
      541 accgctggta ggcaataaaa tattactggc taggtgttca aaattatcaa acactgtgcc
      601 tttccaaaaa gtaaaatctg ataattcatt aaaagacaaa accgtcaata gacccaaagc
      661 ccaaatccct atacctatgg tgatagcgga tactttccta gaaacatttt tttgttcaat
      721 catccagacg acagaaggct caagtaaact aatggcagat gtcagagctg caaaaccaag
      781 taaaataaaa aatagtgagc caaacaaagc tcctcctgat atttgaccga atgcaagtgg
      841 taatgtttta aatatcaagc ccggaccttc agctggttct agaccgtaag aaaatacaat
      901 tggaaaaata atcataccag ccaataatgc aattaaggta tcacaaaaaa taatagcgat
      961 ggtcgattta aagattgact cttctttggg tagataagct ccgtaagtga aaacacaacc
     1021 cataccaaga cttaaagtaa aaaaagcttg ccccatagcc gaaagtaaac ttgaaccatc
     1081 aagttttgta aaatctggcc tgaacatgaa actcaagcct tctaagaaat taccttgagt
     1141 gatggcataa attattagaa ccagcataat aagaaaaagt gcaggcatca tgattgtcac
     1201 tgcccgttca agaccagctt ttataccctt agcgataatt aagatcgtta agaccataaa
     1261 aattgtatga aatataactt gagttttaat tgataaagtt aaggcgttaa aaatttcatc
     1321 aacaccactg ggtcctattc catcaaacac accttgtgct gttaatataa aataattcag
     1381 cgtccaacca gcaatcacac tgtaatagga cagaatcaaa aagcctgcta gtaaaccaat
     1441 cactccggcg tatttccatg actttatccc tgtttcttct aaacctagca gttccatcga
     1501 ggtcatcgga tttcgtctgc ctcttcttcc tagcaatatt tctgaaatca taataggtag
     1561 gcctattgcg aatatacaca acagatagat caatacataa gcgccgccac cattaatgcc
     1621 tgccatgtat ggaaatctcc aaatattacc taaacccact gctgagccac tcacagctaa
     1681 aatgaataca atcctcgatg accattgtcc gtgaagtgat tgacgaagta ttgttgagtc
     1741 tgtgttagtc ataagaagat atagttataa ttaaggatgc aaaataatct taattgaata
     1801 actataaaaa tgacaagaat aattgtagaa aacaagaaag ctaattttaa tttcttcatt
     1861 gaggaaaaga ttgaggccgg catttcccta aaaggttggg aagttaaaag tctaagatct
     1921 aatagaggta atattaaaga aagttatgcc ttagtaaaga atggagagat atggttgata
     1981 ggatcacata tttctcctct tccaaacgta tctgttgatg acgaaaaaga tgctacaaga
     2041 acaagaaaat tactactcaa atctagagaa atctctaaaa tcaatggttt tattactcag
     2101 aaaggatata cactcatccc tctttccttg tactggaaga gtggtagagt caaagtctca
     2161 attggtcttg caaaaggtcg aaaaaagcac gataaacggg ctctcttaaa agaaagagcc
     2221 tggaaattag atcagaaaag attaataaaa aaaaattaca ataattaata tgaaacaaac
     2281 aatgatatat ataacactct ggtctttgtc cttattaaaa tctccagcag ctatttctat
     2341 tgagaattat caccatggga aacatcaaac tatagcaacg atcaattgtc ctcaaaaaca
     2401 agacaccgaa ggaagaaagt atctcagcat gactatgatg catacaaatg agacattcaa
     2461 tgaacttagg tccaatgttc atgtttttaa taagcttagt aatgaacaaa tcatgatggc
     2521 aatgaaatca atgggtccaa actattattg gactctagat aaacaagacc cagatcagac
     2581 aacaggtgcc ctaattttag cccatggtta tggcaaagat ggtgatcgat catttcgaaa
     2641 cagcatggaa cctctatcgt caaaatacat aacctcactc tctttaggaa tgagcatgat
     2701 gacctcaaaa catattgaat gtgcactgct tgatatgcaa cagaaaggtg cagaaaatat
     2761 atatgtcgtt cctattagta gttcatcgcg taattcattg atccaacaat gggaatatat
     2821 atttggatta agaaacgacc acgcttatac caaagttaga tcagttcaaa tggataatgt
     2881 tgttttttta aatccaattg atgaccatct aattgccaag cagatcatat tcgactttgc
     2941 tcaagagatt agtatgaatc ctggaaatga agttgttatc attattgccc atggcccggt
     3001 tagtaaaact aataatgaat ttgaattaga actcttggaa aatattgcca tttatattcg
     3061 tgagtatggg aaattcaatg atgtaagagc ttttaccatc caagatgacg cacctaaaga
     3121 gataaggaat accaacataa atcaaatcag agaatgtata gaggaagcat cattaaatgg
     3181 gaacagcgtt ttgatggttt ccaatctaat gtcaggaaag ggtattcaaa agaaaattta
     3241 tgatgatttt tcaggtttag actataaatt taaccccaaa ggttttctta accatccacg
     3301 atttaatgaa tggatactac agtcgataga atcaaaccat aattagcaaa gcttttatct
     3361 tcttaaggtt cttgagataa tttaatcatt aaaaattatt taaagggggc gacaaggttt
     3421 cgacgttgat attgaaactg tttgtgcatg ccgaggttgc agattcctcg taaatctctc
     3481 tgtaaaaata tagttgcaaa cgacaactac gcactagctg cttaaatcca gctggcatca
//
LOCUS       DASCTH010000313         2215 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000951136, whole genome shotgun sequence.
ACCESSION   DASCTH010000313 DASCTH010000000
VERSION     DASCTH010000313.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000951136"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="QGH35_06560"
     CDS             <1..837
                     /locus_tag="QGH35_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012885.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HJL82223.1"
                     /translation="IKKEFIVRTVDAGIKKIEVTSFVHPKKVPQMADAEKLVESLPDY
                     DDVSYIGLIMNQKGFERAKNCGIQEVGMVIVSTDTYNMRNQGVTTQQSIDNWLEIASE
                     AQSAGIKTNVVIACAFGCPYEGEVDPEKIVEIAEKALQGSPDILGLADSVGVAVPNQV
                     KNVFSLIKELNPDIPLRTHLHNTRNTGLANAAAAIDSGVSIIDASTGGIGGCPFAPKA
                     TGNIPTDDLLYMLDRSNIETGVNLNDVVQTTQWLEEILDRNVPAMVPKAGIFPENANI
                     NL"
     gene            957..>2215
                     /locus_tag="QGH35_06565"
     CDS             957..>2215
                     /locus_tag="QGH35_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007236294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HJL82224.1"
                     /translation="MSYRLGVDVGGTFTDLLLVDEKSGKLYTAKVPSTPDDSSEGVFN
                     GIKRVCDNAGIDPAEIASVMHGTTVATNTVLTGTGAKVGLVTTKGYRQILQIARSFVP
                     GGLGGWVIYNKSDPLAPLALTIEADERTSASGEILRTVKKGKLEKDLKALSKRGIEAL
                     TVSLINSFANDANEKEIKKIINKKKLLPGVPVSLSSEIVPEMQEYERTITTVANSYVR
                     PKVSEYIKNLQKGLKRRMNDVKLHILRSDGGLASAKASENSPINVLMSGPAGGVSGAI
                     WIAQQAGYNNLLTFDMGGTSTDVSLVLNGRAAQSRETTVGDVTVRASSVDVRTVGAGG
                     GSIAHVPELTGALRVGPQSAGAEPGPAAYDKGGVEPTVTDANVALGYMPPNVRLGGDM
                     KIRKDLAVDAVKTVADPLGLSVQATAAG"
BASE COUNT          724 a          406 c          490 g          595 t
ORIGIN      
        1 ataaagaaag aatttatcgt gagaacagta gatgctggga taaaaaaaat agaggtcacc
       61 agctttgttc atccaaaaaa agtgcctcaa atggctgatg ccgaaaaact cgttgaaagt
      121 cttcctgatt atgatgatgt cagctacata ggactcatca tgaaccaaaa gggttttgaa
      181 cgtgcaaaaa actgtggtat tcaagaagta ggtatggtga ttgtcagtac cgatacctat
      241 aacatgagga atcaaggtgt taccactcaa caatcgattg acaattggtt ggaaattgca
      301 tctgaagccc aatcagcagg aattaaaacc aatgttgtga ttgcttgtgc ttttggttgt
      361 ccatatgaag gggaggtgga tcctgaaaag attgtagaaa ttgctgaaaa agccttgcaa
      421 gggagtcctg atattttagg cttagctgat agtgtagggg tcgctgttcc aaatcaagtt
      481 aaaaatgttt tttctctaat taaagaactt aatccagaca ttcctttgag aactcatctt
      541 cacaatacaa gaaataccgg tttagctaat gctgcagctg cgattgattc aggagtctca
      601 attattgatg cttccactgg tgggattggt ggatgccctt ttgcacctaa agcaacaggc
      661 aatattccga cagatgattt gttgtatatg cttgatcgct ctaatattga aacaggtgta
      721 aatctcaatg atgttgttca aaccacacag tggttagagg aaatattaga caggaatgtt
      781 ccagcaatgg ttcccaaagc tggaattttt ccagaaaatg ccaatatcaa tctttgataa
      841 attattattc tataaaacca attcaactat tcacagaatt tactcttcgt tatacaatgt
      901 ttcactcaat gaagatgaaa attcattttg ataaaagata aaaattagga gagaacatga
      961 gttatcgact aggcgtagat gtaggaggaa ccttcacaga ccttcttctt gtagatgaaa
     1021 aatcaggaaa attatacaca gcaaaagtcc ccagcacacc ggatgattct tcagaaggtg
     1081 tttttaatgg aattaaaaga gtctgtgata atgcaggcat tgaccctgct gaaattgcct
     1141 ctgtcatgca tggcacaaca gttgcaacta acaccgtttt aacgggaacc ggtgctaaag
     1201 taggattggt tactacaaaa ggctatcgcc agatactaca aattgcacga tcctttgtcc
     1261 ctggtggttt aggtggctgg gttatctata ataaaagcga cccattggca ccactggcat
     1321 tgactatcga agcagatgag agaacctcag catctgggga aattttaaga acagtcaaaa
     1381 aaggtaaact tgaaaaagac ctcaaagctc tctctaaaag aggcattgag gctctaacgg
     1441 tttcattaat caattcattt gccaatgatg ctaatgaaaa agaaataaaa aaaattatca
     1501 ataagaaaaa actcttacct ggagttccag tctctctttc atcagagatc gttccagaaa
     1561 tgcaagaata tgaaaggacg attaccacgg ttgcaaattc ttacgttcgt cctaaagtct
     1621 ctgaatatat taagaaccta caaaaaggac ttaagaggcg tatgaatgat gtcaaactgc
     1681 atatccttag atcggacgga ggactagctt ctgcaaaggc ctcagaaaac tcgcctataa
     1741 atgtattaat gagtggacct gcaggaggag tatcgggtgc aatctggatt gctcaacaag
     1801 caggctataa caatctgttg acgtttgaca tgggaggaac atctactgat gtttcactag
     1861 tcttgaatgg cagggcagca cagagtcgtg aaaccactgt tggagatgta acagttcgag
     1921 cctcatcagt ggatgtcaga acagtagggg ctggaggtgg atccattgct catgtccctg
     1981 aactcacagg agcattaaga gtcggtcctc aaagtgcggg agcagaacct ggaccagcag
     2041 catatgataa aggcggagtc gaaccaaccg taactgatgc aaacgtagct cttggatata
     2101 tgccacccaa tgttcgcttg ggtggagata tgaaaattag aaaagacctt gcagtggatg
     2161 cagttaaaac agttgcagat cctttgggtc tttctgttca ggcaacagcc gctgg
//
LOCUS       DASCTH010000314         7815 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000961550, whole genome shotgun sequence.
ACCESSION   DASCTH010000314 DASCTH010000000
VERSION     DASCTH010000314.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000961550"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="QGH35_06570"
     CDS             complement(<1..515)
                     /locus_tag="QGH35_06570"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJL82225.1"
                     /translation="MTEAQNTNTENVDVLIVGAGISGIGAAYHLREQCPELSFVILEG
                     LESFGGTWLMHRYPGVRSDSDMYTFGYRFKPWIGPPIATADEILSYMGEVIEEQQLDQ
                     HIRYHHQVTSASWSSEENLWILEGIRKDTGDAVNFTGNFLWMCQGYYEHAKGYTPDWK
                     GMDRFKGEIVHP"
     gene            644..734
                     /locus_tag="QGH35_06575"
     tRNA            644..734
                     /locus_tag="QGH35_06575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:678..680,aa:Ser,seq:cga)
     gene            779..2569
                     /locus_tag="QGH35_06580"
     CDS             779..2569
                     /locus_tag="QGH35_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82226.1"
                     /translation="MSKTSKLTILLSSFTVLLCCYYLIYVTPFFPNQNGGLGHDYALW
                     FPRMLAGVYYFENNGFSIVPWFTPSFCGGNVFFADPQNLFFSIPQFLNFYFGPVFSVT
                     LTFMLFAAFGLVGSYLLAHHTFHLRFSSSIFAAALFLFNGFYAYRIIIGHLSYHSFML
                     IPLICYVLLQPDRTRTWIGEIAISLVAAMLCSYVFYSGGIHLILPLAFSVASVFILFR
                     MCGNNLKFALWRSLSVLVFTMIFCAAKLNVAFATLNTFSRDYYPLSGIDNIFYAFWVP
                     IKSLFISAFTVGETNTIFANSQWAIERHELELSLTIVPLILILWGLIKLFLKDIKISK
                     SQYYCLIPLVIIFSLPIAINFYTPEWNTLLKQTPILKNSVTLVRWYALYIPIIVMVSS
                     LILNKFIRNKSIVFVLIALVIFIKSNEEKSYYAEQSYNPDLVIYAHDQVMKTKVVPKI
                     TEISSSNRVVTPEGIEMPGGDGIMAFGLSQINCSESLFGYRNEEFKQKELLEVNQLVT
                     KTSNKIFNLKNPACYVFPNENNCNPGDHFRIDQVDELLNFTSYKGFNFNMPVRQHIFN
                     WMSLVSFIISSVFFVIFFINSLLFRSLKKS"
     gene            2668..3822
                     /locus_tag="QGH35_06585"
     CDS             2668..3822
                     /locus_tag="QGH35_06585"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HJL82227.1"
                     /translation="MRNFILIVLATFTTYSFSHNGEFYLSQHCPSSFENVNGLCKYRS
                     LYHQYQSLENKGVGGLRTSPPTVRDGFSPQQIDLGRYLFFDPLLSGDHSLSCASCHHP
                     NRGFSDARARSIGANALDVGRASPTLWNVGFLTTFFWDARTHSLEDQMTGPLFAEKEM
                     ANTPENLIQEINANQIYRTLFAEAYPSTDKELITLNQIYTSIAAFEASLISLNSRYDQ
                     YAHGHHQALTLNEIQGLNIFRSFVARCAECHTPPLFTNQQVAVIGTPEPQGRPLDPGA
                     GKIFDSLKLRGGFKVPTLRNINQTAPYMHSGAFTNLRDAVEFYTKGRGHAVPEGEELL
                     LHWHIWEPDLTDNEIDLLVDFLLTLTDESGKPQVPESVPSGLPVVSVETM"
     gene            3858..5378
                     /locus_tag="QGH35_06590"
     CDS             3858..5378
                     /locus_tag="QGH35_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007229434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HJL82228.1"
                     /translation="MNQSRKWTVYFFIVVVFAAGFLIGKLNVDGSGTLIGDVDKYQPS
                     MQARNESETQYGSVTSIEREPFNGQIHTVEQGESIQDAVNDAQPGDVIRILPGTYKET
                     VYIDKDNITLTGVIIKGEWPTLEGEYKLNDAVLYSGNNFTVENLKITHYKGNGIMGQA
                     GNNFIIRNNMILDSGVYGIFPEFGKNGIIEYNVLTGTEDAAIYVGMCDNVHVAHNEVF
                     ANVAGIEIENTRHAIVENNYAHHNTGGILVFITPGLPIKTTYDVIVRDNYVVDNNHEN
                     FGAPGSIVSGIPKGTGILIMAGDDVTLQNNVITGNSNAGIIISDHGSAPNMTLDPGVE
                     PNSDRIAILDNLMYNNGADPTGVVKALMLTKLATRGPDIIRVGESKDSCILNREKYLS
                     FGIDEFGTCAFTSTYGTKTYLLEKPVVMKAINAEERGKLTYYSVCTGCHAYNVRMVGP
                     PTQEIQALYSDNPQGIVDYISNPTRKRADYPEMPPQNYLSMEVRIAAAQFMLDAKQ"
     gene            5571..6500
                     /locus_tag="QGH35_06595"
     CDS             5571..6500
                     /locus_tag="QGH35_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011576609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJL82229.1"
                     /translation="MINKLRLSFLASVIFFISSIFFIPLHSSEIKTQLILLGTGTPNA
                     EPQRSGPSLAVVVGDNSYIVDFGPGIVRRATALSKQWEGNFQALNPKNLKIAFLTHLH
                     SDHTGGYPDLILTPWVLGRETPLEVYGPKGLSKMTELIIEAYKKDIDYRLEGLEPANQ
                     YGWMVNEYEIDEGIVYQDELVEVTAFKVNHGSWDNAFGYKFVTPDKTIVISGDTGPSE
                     NLINYSKEVDILVHEVYSQAGFEKKDEVWKKYHSAHHTSTYELGKIANKIKPDLLVLT
                     HVLYWGSTEKEILDEIYEVYNGPVSFGSDLMVY"
     gene            6517..7320
                     /locus_tag="QGH35_06600"
     CDS             6517..7320
                     /locus_tag="QGH35_06600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJL82230.1"
                     /translation="MALSNNNRTVLITGASAGIGRAFAEVWAEAGFNLLLTARREDRL
                     QEIAHQLQSEQDVKVDILPMDLADPEAPNMIHQHCLDNNIHIDALINNAGYGMSGEFE
                     QMNWESHQNFLQVMVNAVVQLTYLFLPGMIQKKYGRIINLASIAPYLPSPSMAGLYSP
                     VKIFQIKFSESIQIQYLDKGIYCSAVCPGLTRSEFHEAAGMHEVVASAPKWLWMDSRT
                     VAQQGFDAVMKGKTLIINGKLNKAFVILAKFIPENLTRSVAQYLSKRSY"
     gene            7323..>7815
                     /locus_tag="QGH35_06605"
     CDS             7323..>7815
                     /locus_tag="QGH35_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012774.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HJL82231.1"
                     /translation="MHNGITTTFLLQLVAGLILVLPVYGQQDSDWVSGSNISESINET
                     SPIGLVDMPGAEQEDFEDDTLVDSKYLNKQKDQNRDFFITGKARIIDGDTISINKTKI
                     RFSGIDAPESYYYGQTQYCKRSNGKIWACGKKATQKLKELINGQEVQCTDEGKDKYDR
                     TLSI"
BASE COUNT         2386 a         1436 c         1568 g         2425 t
ORIGIN      
        1 ggatggacga tctcaccttt gaatctgtcc atacccttcc aatcaggggt atagcctttc
       61 gcatgctcat aatatccctg gcacatccaa aggaaattgc cagtaaaatt aactgcatcc
      121 cctgtatctt tgcgaatgcc ctcgagaatc cacaagttct cttcactgga ccaacttgct
      181 gaagtaactt ggtggtgata acgaatatgc tgatccaact gttgctcttc aataacctca
      241 cccatatagg aaagaatttc gtcagctgtt gcaatcggtg gaccgatcca aggtttaaat
      301 ctgtagccaa aggtgtacat atcgctatcg gagcggacgc ctgggtaacg gtgcatcaac
      361 caagtgccgc caaagctttc caatccttcc agaataacaa agcttagttc aggacattgt
      421 tctcgcagat ggtacgctgc acctatccca gagattcccg cacccacaat taatacatca
      481 acattttcag tattagtatt ttgtgcttca gtcatttcaa tattttattt attatttatt
      541 gtccaagcta tgatctggat cactcaactt atgatattga tcaatttgct taaataagct
      601 gtgattattt tttttcagat attatatagt cataaaagat gcaggagagg tggctgagtg
      661 gtcgaaagcg gcggtttcga aagccgttga cgggtaatcc tcgtccgggg gttcgaatcc
      721 ctccctctcc gccagagata ataaaatata ataaataata attataaatt attcggtaat
      781 gtcaaaaact tctaaattaa ctatattact aagtagtttt accgtattac tctgttgtta
      841 ctaccttatt tatgtaacac catttttccc taaccagaat ggcggtttgg gccatgatta
      901 tgctttatgg tttccccgca tgctggcagg agtctattac tttgagaata atggcttttc
      961 aatcgtccct tggtttactc catcattttg tggaggtaac gttttttttg cagatcccca
     1021 gaatctcttt ttctccatac cgcagttttt gaatttctac tttggacctg tattttcagt
     1081 aacgcttact tttatgcttt ttgcagcatt cggtttagtt ggtagttatt tgctagccca
     1141 ccatactttt catttgcgtt tctctagttc aatatttgca gctgcattat ttttatttaa
     1201 tggattttat gcctatagaa taataatagg tcatttgagc taccattcct ttatgctaat
     1261 acccctgatt tgttatgttc tgcttcagcc agataggact aggacttgga taggtgagat
     1321 agcaattagc cttgttgctg cgatgttatg tagctatgta ttttattcag gaggtattca
     1381 tcttattttg cctttggctt tttctgtggc ctctgtattt atattattca gaatgtgtgg
     1441 caataactta aagtttgctt tgtggagatc tttgtcagta ttggttttta caatgatttt
     1501 ttgtgcagcc aaactcaatg tagcattcgc aacattgaat accttcagtc gtgattatta
     1561 tcctctttcg ggtatagata atatttttta tgcattttgg gtaccgataa agagtctgtt
     1621 tataagtgct tttacagtag gagaaacgaa cactatcttt gctaactctc agtgggcaat
     1681 agaaaggcat gaattagaat tatcgctaac aatagttcca ttgattttga ttttatgggg
     1741 attgattaaa ttatttctca aagatattaa gattagtaag agccagtatt attgtctaat
     1801 ccctctagtg ataatttttt cattacctat tgcaattaat ttttacactc ccgaatggaa
     1861 tacattactc aaacaaacac caatactgaa aaactctgtc accttagtga ggtggtatgc
     1921 attgtatata cctattatcg taatggtttc gtctttgatc ttgaataaat ttattcgtaa
     1981 taaaagcatt gtttttgtgt tgatcgcatt agtcatcttt atcaaatcca atgaagaaaa
     2041 aagttattac gctgagcaaa gttataaccc tgatcttgtg atttatgcac acgatcaagt
     2101 aatgaagact aaagtagttc caaaaattac tgaaatatca tcatcaaata gggtagtcac
     2161 tcctgaaggg atagaaatgc caggtggtga tgggatcatg gcctttggat tatcacaaat
     2221 taattgctct gaatctttgt ttggttatcg taatgaagaa tttaaacaaa aagaacttct
     2281 agaagttaac cagctagtta caaaaacaag caataaaata tttaatttga agaatccagc
     2341 atgctatgtt ttccccaatg agaataactg caaccccggt gatcatttcc ggattgatca
     2401 agtagatgag ctactcaact ttactagcta taagggtttt aattttaata tgccggttag
     2461 gcagcatata tttaattgga tgagtcttgt gagctttata atttctagtg ttttttttgt
     2521 aatatttttt atcaattctt tgctatttag aagcttaaaa aaatcttgat cagcattaat
     2581 tgatatacaa ttctggatag ttttaaccgt ttaattttat taatctagtt agcttgatag
     2641 cggttttatc tgagttagaa tggagttatg aggaatttta tcttaattgt tttagctact
     2701 tttacgacat attctttttc tcacaacgga gagttttatc tatcgcaaca ctgcccatcg
     2761 agtttcgaga acgttaacgg actttgcaaa tatcgtagcc tataccatca atatcagtct
     2821 ctggagaaca aaggtgtggg tggacttaga acctcaccac caacagtacg tgatggtttt
     2881 tcaccacaac agattgatct agggagatat cttttttttg accccttgct ttcaggtgac
     2941 cattctttgt cctgtgcaag ttgtcaccat ccaaaccggg gtttcagtga tgccagagca
     3001 agatcaatcg gtgccaatgc tttggatgta ggtcgagcgt caccaacact gtggaatgtt
     3061 ggatttctga caacattttt ctgggacgca cgtacacact cattagagga tcagatgacc
     3121 ggtccactct ttgcagagaa agaaatggcc aatactccag aaaacttaat ccaagagatc
     3181 aacgctaacc aaatataccg aacactcttt gccgaggctt atcccagtac agataaagag
     3241 ttgattacat tgaatcagat ttataccagc attgccgctt ttgaggcatc gttgatcagc
     3301 cttaacagtc gctatgacca atacgctcac ggtcatcatc aggctttgac actcaatgaa
     3361 atacaaggct tgaatatttt ccgttccttt gttgctcgtt gcgctgaatg ccacacacca
     3421 cctttgttta ctaatcaaca agtagccgta attggcacac ctgaaccaca aggcagacca
     3481 ttggacccag gtgctggtaa aatatttgat agtcttaaac ttcgtggcgg cttcaaagtg
     3541 ccgactttga ggaacatcaa ccaaactgcc ccttatatgc actctggtgc tttcactaat
     3601 ctaagggatg ccgtggaatt ttataccaaa ggtcgtggac atgctgttcc agaaggtgaa
     3661 gaattattgt tgcattggca tatttgggaa ccagatctca ctgataacga gattgatctt
     3721 ttggtagact ttctacttac actaacagat gaaagcggta agcctcaggt tcctgaaagt
     3781 gtgccttctg ggttaccggt ggtatcggta gagaccatgt gacttaatcg attggcacat
     3841 gcaataggag aaaaatgatg aatcaatcac gaaaatggac tgtttatttt tttatcgtgg
     3901 tggtttttgc tgcgggtttt ttaattggta agttgaacgt tgatggatcc ggaacattaa
     3961 ttggtgatgt cgacaaatac caaccatcta tgcaggcaag gaatgagagt gaaacacagt
     4021 acggttcagt tacaagcatc gaacgcgaac catttaacgg acaaatccat accgttgaac
     4081 aaggagaatc tatccaggat gcagttaatg atgcacagcc cggtgatgtt atcagaatcc
     4141 tacctgggac ctataaggaa actgtctata tagataaaga caacattact ctgacgggtg
     4201 taattattaa aggagagtgg ccaactcttg aaggagagta taagttgaat gatgcggtct
     4261 tatattccgg taacaacttt actgtagaaa acctgaagat aacccattat aaaggcaatg
     4321 gcattatggg tcaggcaggt aacaatttta ttattcgcaa taacatgatt ctagactctg
     4381 gtgtctacgg aatttttccc gagtttggaa aaaacggcat catcgaatat aacgtactaa
     4441 ctggcaccga agatgctgcg atctatgttg ggatgtgcga caacgtccat gttgctcata
     4501 atgaagtctt tgctaatgtc gctggcattg aaatcgagaa cacccgccat gctatcgtag
     4561 aaaacaatta cgctcaccac aataccggcg gtattttagt tttcattacc ccaggcttac
     4621 caatcaaaac cacttatgat gtcattgtta gagataacta tgtagttgat aataaccacg
     4681 aaaactttgg tgcacccggt tccattgttt ctggaatacc caaagggact ggcatcctga
     4741 ttatggcagg ggatgatgtg accctgcaga ataatgtcat tactggtaac agtaatgctg
     4801 gcatcatcat ttctgatcac ggtagtgccc cgaatatgac actggaccca ggagtcgagc
     4861 ctaactctga tagaatcgca atccttgata acctgatgta taacaacggt gctgacccta
     4921 caggagtagt gaaagcattg atgctcacca aactagcaac acggggaccg gatattattc
     4981 gtgttggtga aagcaaagac agttgtattc ttaatcgtga aaagtacctc agttttggca
     5041 tagatgaatt tggcacttgt gcttttacta gcacctacgg cactaagacc tatttgctcg
     5101 aaaagcctgt agtgatgaag gcaatcaatg cagaggaacg aggcaaacta acttactaca
     5161 gtgtttgtac tggttgtcat gcttacaacg taagaatggt tggaccgcca actcaagaga
     5221 tccaggcact ctattctgat aatcctcaag gtattgttga ttacatcagc aatccaacta
     5281 ggaagcgagc tgattatcca gaaatgccac cacagaatta cttatccatg gaagttagga
     5341 tagccgcggc acaattcatg cttgatgcta aacagtagga ctgacacttt gtagttatta
     5401 gtcaatataa atattagaga attaatggaa aatgattgat tcggaaagta aacctaaggt
     5461 tagaaaaaga actccatccc acgcttcaac taaaaagcac aaaaataaga aaacatataa
     5521 tcgaaaggaa aaaggaagaa aaatagatac cagaatcaaa ggaataattg atgatcaata
     5581 aattacgtct ttcctttcta gcttcggtca ttttttttat ttcctcaata ttttttattc
     5641 cactgcactc gagtgaaata aaaactcaat taatacttct tggaacggga acacccaatg
     5701 ctgaacctca acgatcaggt ccatccctag ctgttgtggt tggagacaat tcttatattg
     5761 ttgattttgg tccaggcatt gttaggaggg caacagcact ttcaaaacaa tgggagggca
     5821 attttcaagc attgaatcca aaaaacttaa aaatagcttt cttaacacac ctacattcag
     5881 atcataccgg aggatatcct gatcttatac taaccccctg ggttttaggt agagagacac
     5941 ctttggaagt ctatggtccg aagggcttgt cgaagatgac tgaattgatt atcgaagcgt
     6001 ataagaaaga cattgattac cgtctagaag gtcttgaacc agccaatcaa tatggttgga
     6061 tggttaatga gtatgaaatc gatgaaggta ttgtttatca ggacgaatta gttgaagtta
     6121 ctgcttttaa agtcaaccat ggatcttggg acaatgcttt tggttacaaa ttcgtcactc
     6181 ccgataaaac catcgttatt tctggggata caggacctag tgagaatttg attaactata
     6241 gtaaagaggt tgatatttta gtacatgagg tttattcaca agctggtttt gagaagaaag
     6301 atgaggtttg gaaaaagtat cattcagcac atcatacttc gacctatgag ctgggtaaga
     6361 ttgcaaacaa aattaaacca gatttattgg ttttaaccca cgtactttat tggggctcaa
     6421 ctgagaaaga aatattagat gagatttatg aagtatataa tggtcctgtt tcttttggat
     6481 cagatttaat ggtctattga ctaaccagag gttgaaatgg cactaagtaa taacaatagg
     6541 actgtcttaa ttacaggcgc atccgcaggg ataggcagag cttttgccga agtttgggct
     6601 gaggctggat tcaatctttt gctgactgct cgaagggagg atcgactcca ggagatcgcc
     6661 catcaattac agtcagaaca agacgttaaa gtggatattt taccgatgga tttagcagat
     6721 cctgaagctc ctaacatgat ccatcaacac tgcttggata acaacattca tatcgatgct
     6781 ttgatcaata atgcaggtta tggtatgtct ggagaatttg aacaaatgaa ttgggaatct
     6841 catcagaatt ttttacaagt catggtcaat gctgttgtgc aactgaccta tttatttctt
     6901 ccagggatga ttcaaaaaaa gtatggaagg attattaatc ttgcctcaat agcaccgtac
     6961 ctaccatcgc caagtatggc gggtttatat agtcctgtga aaatttttca aattaaattt
     7021 tctgaaagca ttcaaattca atatcttgat aaagggattt attgttcagc tgtctgtcct
     7081 ggattaacca gatcagagtt tcacgaagca gctggaatgc atgaagtggt tgccagtgca
     7141 cccaagtggc tgtggatgga ttctagaacc gttgcgcaac aaggttttga tgctgtaatg
     7201 aaagggaaga ccttaattat taatggaaaa ttgaataaag cttttgtgat tctggcaaaa
     7261 tttattcctg aaaatttaac aagatcggtg gcacaatatc tatctaaaag gtcttattga
     7321 tcatgcataa cggaattaca acaacttttt tattacagct cgtggcggga ttaatcttgg
     7381 tgttgcctgt ttacggacaa caagatagtg attgggttag cggtagtaat atatctgaat
     7441 caattaatga aacatcacca attggattag tggatatgcc cggggcagaa caggaagatt
     7501 ttgaagacga tacattggtt gattctaaat atcttaataa acaaaaagat caaaatagag
     7561 attttttcat tactggtaaa gcaaggatca tcgatggtga caccatcagc atcaacaaaa
     7621 ctaaaattag atttagtggt atcgatgccc cagaaagtta ctattatggc caaacccaat
     7681 attgcaaaag aagtaatgga aagatatggg cttgtggaaa aaaggccact caaaaactta
     7741 aagaattaat caatgggcaa gaggttcaat gcactgacga aggtaaagat aagtatgatc
     7801 gaacactcag tattt
//
LOCUS       DASCTH010000315         1630 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000840969, whole genome shotgun sequence.
ACCESSION   DASCTH010000315 DASCTH010000000
VERSION     DASCTH010000315.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000840969"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1630)
                     /locus_tag="QGH35_06610"
     CDS             complement(<1..>1630)
                     /locus_tag="QGH35_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012602.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HJL82232.1"
                     /translation="YPLKRKGKRGEGKWQRISWDQATTEIADKFLDYATQYNPECISY
                     ASGTQMSVKMASFASLLRFGNITGVTVPEFFSGVGDLPTGSHMTLGQVYTGDTFASVY
                     KSKCVLVWMSNPAVTRIPDAHFFWEARYNGTQVIAISPEFTPTAMHSNLWLNPKPGTD
                     SALAMAMAQVILSEKLYQPDYIKEQSDLPLLVRTDTKELLRKEHLSLYGLLAVADNVY
                     YMWNNKTNAMVRAPGTGRADMPIGRDRRKQGTLDLGDIQPALEGRWLVKTLDGEIEVT
                     TVFEMLKEQCKDYEPEKASAITGISADAIRKTARVFAKAQPAMIYAGYASCKWLHGDL
                     LQRAMLLLLSLTANIGREGGGLQVANAPIARGMNQFGFSDIGPAFRLISGTTWDYDHG
                     NMKALNTKIYGQELADTYDSYYQESIAEDWFPDYSKHGWKMGIFAGNNGANWRAAGSA
                     WRKNAFEELETIVSLAPDMGVTSFYSDYVLPIAHHYERNDLMLQSRVPYLQVLSEAVP
                     PLGESVDDWEANKRLAAAISKRAKERGIAPIKDAV"
BASE COUNT          423 a          398 c          326 g          483 t
ORIGIN      
        1 accgcatcct tgattggagc aatgcctctc tcctttgcac gtttactgat tgctgctgcc
       61 aaccttttat tggcctccca atcatcaaca gactccccta gtggtggcac tgcttcactc
      121 agaacctgca gataggggac tctggattgc aacatcaaat catttctttc atagtgatgg
      181 gcaattggca gtacatagtc tgagtagaat gatgtcaccc ccatatcggg tgccaatgaa
      241 acaattgttt ccagttcttc aaaagcattt tttctccaag cactccctgc agctcgccaa
      301 tttgccccat tgttgcccgc gaatataccc attttccatc catgtttgct gtaatctgga
      361 aaccaatcct ccgctatact ttcttgatag taactgtcgt aagtatcagc taattcttgg
      421 ccatatatct tggtattcaa tgctttcata ttgccatgat cataatccca agtggtgcct
      481 gaaattagcc taaaagcagg acctatatcg gaaaaaccaa attgattcat gcctctggca
      541 ataggggcat tagcaacctg gagaccgcct ccctctctgc caatattagc cgttagagag
      601 agcagcaaca acatggctct ttgtagtaaa tcaccatgta accacttaca agatgcgtat
      661 cctgcataaa tcattgctgg ctgagctttt gcaaatactc ttgctgtttt tctgatggca
      721 tctgcgctga tacctgtaat tgcagaagct ttttctggct catagtcttt acactgctct
      781 tttagcattt caaaaaccgt ggtcacctct atttcaccat ccagtgtttt gactagccaa
      841 cgaccttcca gggccggttg gatatcacct aaatcaagcg ttccttgctt tctacgatcc
      901 ctgcctatgg gcatatcggc gcgaccagtt ccaggagccc gaaccatggc attggttttg
      961 ttgttccaca tgtagtagac attgtcggca actgccagca aaccgtatag tgagagatgt
     1021 tcttttctga gaagttcctt agtgtccgtt cgtaccagaa gtggcaaatc actctgctct
     1081 ttaatgtagt caggttgata tagtttttca ctgagaataa cctgagccat tgccattgcc
     1141 aaagcactgt cggtgcccgg ttttggattg agccaaagat tcgaatgcat cgccgttggt
     1201 gtaaactcag gcgaaattgc aatgacttga gtgccgttat agcgtgcttc ccaaaaaaaa
     1261 tgagcatcag gaattcttgt tacagcagga ttgctcatcc aaaccaaaac acattttgat
     1321 ttgtaaactg aggcaaatgt atctcctgta taaacctggc caagagtcat gtgcgatcca
     1381 gtaggaagat cgccaactcc tgagaaaaac tcaggcacag taacgccggt aatatttcca
     1441 aaccttagta aagatgcaaa agaggccatc ttaaccgaca tttgagtgcc gctggcgtac
     1501 gatatgcatt cgggattata ttgcgttgca taatccagga atttatcagc aatttcagta
     1561 gttgcttgat cccagctgat tctttgccat ttaccttcgc cccgtttacc ctttctttta
     1621 agaggataga
//
LOCUS       DASCTH010000316         2341 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000717446, whole genome shotgun sequence.
ACCESSION   DASCTH010000316 DASCTH010000000
VERSION     DASCTH010000316.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000717446"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="QGH35_06615"
     CDS             <1..894
                     /locus_tag="QGH35_06615"
                     /inference="COORDINATES: protein motif:HMM:NF018979.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HJL82233.1"
                     /translation="NPDLYIAAVSSDERLEAFPNAPTFAELGVTTLNKEYMWRGFALK
                     AGTPPDVIEWYTNLFQQVTADPDWKEFWEKGGIDVEFIGGDEFTDIVKQDIETFEYYL
                     TKSEIISTFSNNGLSKYGEGTPLLILTIGLITVIALAGYLIYKSSFSNTFGRIMVIAF
                     FLLISIVFYLQSFIFPVSREVGPGVIPRLWIFTLVPLSLLLLVKTLRNKDGVEQAGPR
                     VDIVLTFIGFLVVYLLVMNYIGYFLSTFIFMITGMIYLGYKNIKVMLITSATWIVFSY
                     IIFYKILFVPLPLGKWFEGLF"
     gene            894..>2341
                     /locus_tag="QGH35_06620"
     CDS             894..>2341
                     /locus_tag="QGH35_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HJL82234.1"
                     /translation="MELLSNLGDGLSYVIGVVPILTIILGVTVGIIAGATPGLSPSMG
                     VALLVPFTYAMSPTLALILLVAIYIAANYGGSITAVTINAPGTPSSVVTSFDGYPLTK
                     KGKPGMALGVSLVASTIGGIVGTLILIFFSGPLAKVALKFHPAEYFALAIFGLTTVAS
                     LGGKNWIKAFIAAMFGLLINTIGIDPISGVSRFTFGLSFLYDGFALIPALIGLFALSE
                     IFKQLETGKFDSKAADSGKQKWPTFLEYWKLKLTILRSSVIGTIIGIFPGAGATIAAF
                     ISYDLTQKLSKTPGKFGKGSLEGVAAAEGANSSSVGGALIPLLTLGIPGSASTAVLIG
                     ALMIHDLTPGPQLFISSPDIIYGLLSSLLLANIVLLALGFFGSRLWIKVTIIPKRILF
                     PIIFAVSIIGSFAVRNSFFDVIACLGFGVFGWILKRYNYPVAPIVLGMVLGNIAETNF
                     IRAVMMDGWAVFFTRPASLIMLLVALASFAVP"
BASE COUNT          645 a          400 c          488 g          808 t
ORIGIN      
        1 aaccctgatt tgtacattgc tgcagtttca agtgatgaga ggttagaagc cttccctaat
       61 gcaccaactt tcgctgaact tggtgtgact acactaaaca aggaatatat gtggcgtggg
      121 tttgccctaa aagcaggaac accacctgat gttattgaat ggtatactaa cctattccaa
      181 caagttacag cagatcctga ttggaaagaa ttttgggaaa agggtggtat tgatgttgaa
      241 ttcattggtg gggatgagtt tactgatatc gttaagcagg acattgaaac attcgaatat
      301 tatctaacga agtcggagat tatttctact tttagtaata acgggttaag taaatatgga
      361 gaggggacac ctttattaat tttaaccata ggtttgatta ctgtcattgc tttagcggga
      421 tacttgatat ataaatcgtc gttctcaaat acttttggcc ggataatggt aattgccttt
      481 ttcctattaa tttcaatagt attttatcta caatctttca tttttccggt gagtcgtgaa
      541 gttggaccag gtgttattcc tcgtttatgg atatttacct tagttccact ttctctttta
      601 ctattagtaa aaacgttaag gaataaagat ggtgtagagc aagcaggacc gcgagttgat
      661 attgttctaa catttattgg cttccttgta gtttatttat tagtgatgaa ttatatcggg
      721 tatttcctaa gcacttttat ttttatgatt accggaatga tttatttagg ttataagaat
      781 ataaaagtaa tgctcattac atctgcaaca tggattgtgt tttcatatat cattttctat
      841 aagatcttat ttgtaccact tccgcttggt aagtggtttg agggattatt ctaatggaat
      901 tactttcaaa tttaggtgat ggactttcat acgtaattgg agtcgtacct atccttacaa
      961 ttattttggg tgttacggtg ggtattattg caggagcgac accggggctt tctccttcga
     1021 tgggagtggc cttactagtt ccctttacat atgcaatgtc tccaacttta gcactaatat
     1081 tacttgtcgc aatttatatt gctgcaaact atggtggttc aattacagct gtaacaatca
     1141 atgcgcctgg cactccatca tctgtagtta catcatttga cggataccca ttaactaaaa
     1201 aaggtaaacc gggtatggca cttggcgtat cgctagtagc atcaacaatt ggaggtattg
     1261 taggtactct aatattaata tttttctcag gtccgcttgc aaaagtcgct ttgaaatttc
     1321 atccggctga gtattttgcc ttagcgatat tcgggctcac cacagtagca tcacttggtg
     1381 gaaaaaattg gatcaaagca tttattgcag caatgtttgg actactgatt aatactatag
     1441 gtatagatcc gatttcagga gtaagccgat ttacatttgg gctatctttc ttatatgatg
     1501 gattcgcttt gatacctgcg ctgattggtt tatttgcatt aagcgaaata tttaaacaat
     1561 tggaaaccgg aaaatttgac agcaaagctg ctgattcggg gaaacaaaaa tggccaactt
     1621 tcttagaata ttggaagcta aaattgacca ttttgcgctc ttctgttata ggtactataa
     1681 tcgggatatt cccgggagca ggggcaacaa tagctgcatt tatatcctat gatttgactc
     1741 aaaaattgag caaaacacct ggtaagtttg gaaagggcag cctggaagga gtagcagctg
     1801 cagaaggagc aaacagtagt tccgttggcg gtgcattaat tccattactg acattaggaa
     1861 taccgggcag tgcctccact gccgtactga tcggagcgtt aatgattcac gatctcactc
     1921 ccggtccgca actatttatt tcgagtcctg acattatcta tggtttactt tcaagcttat
     1981 tattagcaaa tattgtttta ttagcgcttg gattctttgg cagtcgatta tggattaaag
     2041 taactataat tccaaaacgt atattattcc caataatatt tgctgtatca atcatcggga
     2101 gttttgccgt tcgtaattca ttctttgatg taatagcctg tttaggattt ggtgttttcg
     2161 gttggatcct aaaacgctat aattatccgg ttgctcctat agtcctcggt atggtgctag
     2221 gaaatattgc agagactaat tttatccgtg ccgtcatgat ggatggatgg gcagtattct
     2281 ttacgagacc tgcaagttta attatgttgt tagtagcact tgctagtttt gctgtacctt
     2341 t
//
LOCUS       DASCTH010000317         1709 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000137964, whole genome shotgun sequence.
ACCESSION   DASCTH010000317 DASCTH010000000
VERSION     DASCTH010000317.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000137964"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="QGH35_06625"
     CDS             complement(<1..249)
                     /locus_tag="QGH35_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HJL82235.1"
                     /translation="MNKHEKFIRLIHMGRIPGWQTQAVYHTVAELMTEDTPDTIIISQ
                     PSDTYVCLGYHQKLDEIFNRNVCDEKEIPILRRKVGGGG"
     gene            complement(246..1349)
                     /locus_tag="QGH35_06630"
     CDS             complement(246..1349)
                     /locus_tag="QGH35_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJL82236.1"
                     /translation="MNVEQIKLSSQEQEELLNISPDYVRISMAAAIELGLKPGRIHGC
                     GCGCINLLQNYPEGCFANCSYCGLARERPGLAEENSFIRVDWPLFSTDLIAEKLAEKE
                     AENSVGRVCISQVQDSRANDDLIKITRKVHIAAPEVPLSGLVNATTMTVPFLEQLKEE
                     GIDIIGYGLDAVTEELFIKTRGKEASGPHDWDYHWEMVRAARKIYGPMNVNCHLIVGL
                     GETDRDLVDMLYRLKSEEIAGYLFSFNPEPGTTLQYQTQQPIKRHRRVQLAKYLIEEK
                     NVERNVFQFDENGFITSIDSDEDLIQNTINEGNPFMTNGCPDRDGVMACNRPYGSYRP
                     GEEFRDYPFKPTGDELTVIREQMQLNGTLGNPV"
     gene            complement(1359..>1709)
                     /locus_tag="QGH35_06635"
     CDS             complement(1359..>1709)
                     /locus_tag="QGH35_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJL82237.1"
                     /translation="KMNGEWAALDMIKKYPPKILVLVILLPLTGTGMEGVIPPSMDEI
                     GVFFETARLLLPTTPILLGCARPLGNMKIEIDKSAINAGLNGIAFPSEGIVGYAQEKG
                     LEPSFINACCGVTW"
BASE COUNT          477 a          399 c          289 g          544 t
ORIGIN      
        1 tccaccacca ccaacttttc tacgtaaaat gggaatctct ttttcatcgc agacatttcg
       61 gttgaatatt tcatccagtt tctgatgata gcctaaacat acatatgtat cggatggttg
      121 agagataata atagtatccg gtgtatcttc cgtcatgagt tccgcaacgg tgtgatatac
      181 cgcctgcgtt tgccaaccgg gaattcgacc catgtggatt aaccgaatga atttttcgtg
      241 tttgttcaaa ctggatttcc taatgttcca tttaattgca tttgctcacg gataacggtt
      301 aattcatcac cagtgggttt aaaaggataa tctcgaaatt cttcacctgg acgatatgaa
      361 ccataagggc gattacaggc catcacaccg tcacgatcag gacagccatt tgtcataaac
      421 gggtttcctt cattaatggt attttgaatt aaatcttcat cagaatcaat ggatgtaata
      481 aacccattct catcaaattg aaatacattt cgctccacgt ttttttcttc aatgagatat
      541 tttgccaact gcacccgacg atggcgttta atgggttgct gtgtttggta ctgcaatgtg
      601 gttcctggct ccggattaaa tgaaaataaa taccctgcaa tttcttctga ctttaatcga
      661 tataacatat ccactaaatc acgatctgtc tctcccaaac ctacaatcaa atggcaattt
      721 acattcatag ggccataaat ctttcgtgct gcccgaacca tttcccaatg gtaatcccaa
      781 tcatggggac cactggcttc cttaccgcga gtttttatga ataattcttc tgtaacagcg
      841 tccaatccat acccaataat atcaatccct tcttccttca attgctccaa aaaaggaaca
      901 gtcatggttg tggcatttac caatccggat agtggaactt ctggtgctgc gatgtgcacc
      961 tttcgagtaa tttttattaa atcatcattc gctctagagt cttgaacttg agaaatacag
     1021 acacgcccca cggaattttc tgcttctttt tctgcaagct tttctgcaat taaatctgtg
     1081 gaaaataggg gccaatccac gcgaatgaaa ctgttttctt ctgctaatcc gggacgctct
     1141 cgtgctaatc cacaataact gcaattggca aaacagcctt ctggataatt ttgcaataaa
     1201 ttgatacatc cacatccaca accgtgaatt cggcccggtt tgagtcctag ttctatggca
     1261 gccgccatac taatccgaac ataatccgga ctaatattca gcaattcttc ttgttcttgt
     1321 gatgataatt ttatttgttc tacattcata tttctccctc accaagtcac gccacaacag
     1381 gcattaataa atgatggttc taatcctttt tcttgcgcat atcccacgat accttctgat
     1441 ggaaatgcaa tgccattcaa tccggcatta atagcagatt tatcaatttc aattttcata
     1501 tttcccaacg gacgagcaca tcccaataaa atgggtgtcg tcggcaacaa caatcttgct
     1561 gtttcaaaaa agacccctat ttcatccatg gatgggggaa tcacgccttc catacccgta
     1621 cctgttagtg gtaataatat cactagcact aatatttttg gtggatattt tttaatcatg
     1681 tccaatgcag cccactcgcc attcatttt
//
LOCUS       DASCTH010000318         2853 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000469785, whole genome shotgun sequence.
ACCESSION   DASCTH010000318 DASCTH010000000
VERSION     DASCTH010000318.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000469785"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            34..2124
                     /locus_tag="QGH35_06640"
     CDS             34..2124
                     /locus_tag="QGH35_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HJL82238.1"
                     /translation="MLSPNEQIAFILLVVVCGRLAFQGFNRIFKTVKSGAKADRSDNL
                     VGRFMNALIDVFLQKPIAKARPIVSMFHSFIFFGFSFYLLVNMNDVLEAYVDGWTTIG
                     SDNIVAKLFNVFADIFSVFVLVGMIFFLYRRFVQKPKVMEFNTNVKLHPGVSAGGLKK
                     DSLIVGIFILIHVGSRWLGTAMHLAGNGHTDSWLPTASIISGLFSNWSHASLETGIHV
                     TWWLAMGLIVMFLPYFPRSKHIHLMIAPVNLALGRKTARGAMDGISNPASPGSKTLSD
                     LPWPQVLDPWACIMCTRCHEVCPAHESGTPLSPSALEINKRYFINEQGNALFNDAGET
                     ALMDYAISDDAVWSCTTCYACVEVCPVGNEPMMDIMEMRRNQVFDAKMPDELADVLRS
                     LDEQGNSFNESARRRHKWSKKLDIKLKDATKEPVKYLWYVGDFASFNQNCQDTTRKVA
                     EVLTAAGVDFGIMGKKEKSAGNDVRRTGEEGLFEVLAEQNIKMLEKCDFEEIFTTDPH
                     TFNTLKNEYPDKGGNYTVKHYSTLLLELIQSGDIDLKKKLDIKATYHDPCYLGRYNGI
                     YNDPRDVMKLCGIDLVEMPRNKENSFCCGAGGGRVWLPDHDDMTQRPSENRIEEAVAV
                     GVNNFTVACPKDMTMYADAAKTSGNEDTMAVKDIIDFVLEAMDLPEPIMEEIASSYPN
                     EEVSTEPQSEGLDL"
     gene            2121..2555
                     /locus_tag="QGH35_06645"
     CDS             2121..2555
                     /locus_tag="QGH35_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013743.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HJL82239.1"
                     /translation="MKKQFDFKSDRFYNSNHFWSKQDDAGIVTVGMDELGLDSLGEMA
                     YLTLPAVGTPVEMGKVMGSMEAAKMTGELFAPISGIVVEKNDAVLQNPLLVNEDPFDK
                     GWLIKIEPANWEEESGAMVSNDELSAWMESEIERFETQGMAG"
     gene            2552..>2853
                     /locus_tag="QGH35_06650"
     CDS             2552..>2853
                     /locus_tag="QGH35_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HJL82240.1"
                     /translation="MNTWRYIENNGVTASDGLAGDEALANRVGAGISQPTLRLYTYKP
                     CALVGRFQTIENELNLEYCAQNKIPVNRRPTGGGAIIMGENQLGVALAIPGKTDES"
BASE COUNT          841 a          501 c          690 g          821 t
ORIGIN      
        1 gaagaattgg ttgaagagct ggaagattaa tctatgttat ccccaaacga acaaatcgca
       61 tttattctat tagttgtcgt ttgtggcaga ttggcctttc aggggtttaa tcgaatattc
      121 aaaacggtta aatcgggagc aaaggcagat agaagtgata atttggttgg tcgatttatg
      181 aatgccttga ttgatgtgtt tcttcaaaag cccattgcca aagctcgacc gattgtatcc
      241 atgtttcatt cattcatttt ctttggattt agtttttatc ttctggtaaa tatgaatgat
      301 gttttggaag cgtatgtgga tggttggact actattggaa gtgataatat cgttgccaaa
      361 ttgtttaatg tatttgccga tatttttagc gtgtttgtat tggttggaat gatattcttt
      421 ctttatcgcc ggtttgttca aaagcctaaa gtcatggaat tcaatacaaa tgtaaagtta
      481 catcccggtg taagtgccgg tggattgaaa aaagattcac ttattgtggg cattttcatt
      541 ctcattcatg tgggttcaag gtggctcggg accgccatgc atttagcggg aaatgggcac
      601 acagattctt ggctgcctac cgcaagtatt atttcaggac tattttctaa ttggtcccat
      661 gcgtcattgg aaacgggcat tcatgtaacg tggtggttgg caatggggct catagttatg
      721 tttctgccat actttccacg atctaaacat attcatctta tgattgcacc ggtgaatttg
      781 gcgttgggcc gcaagactgc tcgcggtgct atggatggga tttcgaatcc ggcttcaccc
      841 ggttccaaaa cattatccga tttgccctgg ccgcaagtgt tagacccatg ggcctgtatc
      901 atgtgcactc gctgtcatga agtttgtccc gcacacgaaa gtggtacgcc attaagtcct
      961 tccgcattgg aaatcaataa acgctatttc attaatgaac aagggaatgc cttgtttaat
     1021 gatgccggtg aaacagcctt gatggactat gctatttccg atgatgcggt ttggtcttgt
     1081 accacttgtt acgcttgtgt ggaagtgtgt cccgtaggaa atgagcccat gatggacatt
     1141 atggaaatgc gtcgaaatca agtatttgat gcaaaaatgc cggacgaatt agccgatgtg
     1201 ttacgaagtt tagacgagca aggaaattcg tttaacgaat cggcacgccg tcgtcataaa
     1261 tggtctaaaa aattagatat caaactgaaa gatgccacaa aggaaccggt gaaatattta
     1321 tggtatgtag gtgactttgc gagtttcaat caaaattgtc aagatacgac acgaaaagta
     1381 gcggaagtat taacagccgc cggtgtagac tttggcatca tgggtaaaaa ggaaaaatct
     1441 gcagggaatg atgtacgacg taccggtgaa gaaggactct ttgaagtgtt agcagagcaa
     1501 aatatcaaaa tgcttgaaaa atgtgacttt gaagaaattt tcacaactga tccacacact
     1561 tttaatacgc tgaaaaatga atatccggat aaaggcggaa attataccgt gaaacattat
     1621 tccactttat tgctggaatt gatccaaagt ggtgatattg atttaaagaa aaaattggac
     1681 ataaaggcta cttaccatga tccatgctat ttgggtcgat ataatggtat ttataatgat
     1741 ccacgagatg tgatgaagct ctgcggaatt gatttggtgg aaatgccccg taacaaagaa
     1801 aatagttttt gctgtggtgc aggcggtgga cgggtttggc ttccagacca tgatgatatg
     1861 actcaacgtc ccagcgaaaa tcgcatcgaa gaggcagtag cggttggtgt aaataacttc
     1921 actgtggcgt gtccaaaaga tatgaccatg tatgccgatg cagcaaaaac atcggggaat
     1981 gaagatacga tggcggtgaa agatattatt gattttgtcc tggaagccat ggatctgccc
     2041 gaaccaatca tggaagaaat tgccagttca tatccgaacg aagaagtatc aacagaacca
     2101 caatctgaag gattagactt atgaaaaagc aattcgattt taaatcagac cgtttttata
     2161 actcaaacca tttctggtca aaacaggatg atgctggtat tgttacagtt ggcatggatg
     2221 aactgggttt ggattctctg ggcgaaatgg cctatttaac tctgccggct gtagggacac
     2281 ccgttgagat gggaaaagtc atgggttcta tggaagcggc aaaaatgact ggtgagcttt
     2341 ttgctccaat ttccggaata gttgttgaaa aaaatgatgc tgttttgcaa aacccgttgt
     2401 tggtcaacga agacccattt gataaggggt ggttgattaa aattgaaccg gcgaactggg
     2461 aagaagaatc aggtgcaatg gtctccaacg atgaactttc cgcatggatg gaatctgaaa
     2521 tagaacgatt tgaaacccaa ggcatggctg ggtgaacact tggcggtata tagaaaataa
     2581 tggtgtaacc gcatccgatg gattagcggg tgatgaagca cttgccaatc gtgttggtgc
     2641 aggcatatct caacccacac ttcgacttta tacttacaaa ccttgcgcat tagtagggcg
     2701 atttcaaacc attgaaaatg aattgaattt ggaatattgc gcccaaaata aaatccctgt
     2761 caatcggcga ccaacaggcg gtggtgctat cattatggga gaaaatcagt taggtgtagc
     2821 tttagcgata ccagggaaaa ccgatgaatc tta
//
LOCUS       DASCTH010000319         2792 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000053529, whole genome shotgun sequence.
ACCESSION   DASCTH010000319 DASCTH010000000
VERSION     DASCTH010000319.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000053529"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="QGH35_06655"
     CDS             <1..312
                     /locus_tag="QGH35_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HJL82241.1"
                     /translation="FVCDIGGMVVICAGTSGYNITMDLRYHWMMQKRLQGSHLANDTE
                     AEALNQLVLARKVDPCLSETYSFDEIGYAHQLMHENKHPYGNMACLVNATEKGQGKTE
                     D"
     gene            324..953
                     /gene="rsmG"
                     /locus_tag="QGH35_06660"
     CDS             324..953
                     /gene="rsmG"
                     /locus_tag="QGH35_06660"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HJL82242.1"
                     /translation="MAKQEDLLKQGLQQIGLNTEQQNIDLLIAYINLLLEQNRTLNLT
                     AIIDPLEAVTKHLLDSLSIAQDISEGTVVDVGSGGGCPGIPLAIIKPESQFVLVESKN
                     KKANFLKTTTQTLKIKNIKVLCQRAENIKLTKKADTIVSRALGGLPYFVSVAGHMLKK
                     QGRLLAMKGKIPHSELKEIPLGWEVVKTKKIEVPYLQAERHVLIIARQM"
     gene            955..1707
                     /locus_tag="QGH35_06665"
     CDS             955..1707
                     /locus_tag="QGH35_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJL82243.1"
                     /translation="MAKTIAIANQKGGVGKTTTAVNLAAALGSMGKKTLLVDLDPQSN
                     ATSACGVEKNKLNSSIYDLFVGEGAVEATILFSEKNGFWILPANKDLLAAEIQLLEQH
                     EREFQLKKILEKATDNFDYILIDAPPSMNILTLNALAAAGAVIIPVQCEYYALEGLTG
                     LLETINQVKQKINPSLEIKGVLRTMFDVRNNISVEVSVQLKKHLGNKLLWTSIPRNVR
                     LAEAPSFGLSALAYDQGCLGAKAYLSLAKEIT"
     gene            1753..2637
                     /locus_tag="QGH35_06670"
     CDS             1753..2637
                     /locus_tag="QGH35_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011576981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HJL82244.1"
                     /translation="MDEKKKSLGRGLEALLGASEPAQQTIKPDEAIHQLSIEKIGPGP
                     FQPRRTIREEQLKELALSIEEQGIIQPIVVRERAVVDSQTGVRFEIIAGERRWRAAQL
                     ANLGAVPAVIRTMSDASAVAVALIENIQRENLNPLEEANAFQKLIIEYEMTHQEVANA
                     VGRARASITNTLRLLDLSSSVQALLNEAKINMGHARTLLSLTDSSMQLEVANLIIEKK
                     LSVRDTESLVRSILEGANKPKTKKQPEDPDIKRLESSLTQKLGAKVFIKHAKSGKGRL
                     VIAYNSSDELQGILDRIR"
BASE COUNT          961 a          588 c          623 g          620 t
ORIGIN      
        1 ttcgtgtgcg acattggcgg gatggttgtg atctgtgcag gaacctcagg atacaatatc
       61 acgatggacc ttaggtacca ttggatgatg caaaaacgcc tacaaggctc acaccttgca
      121 aatgacaccg aggcagaagc attaaaccaa ctggttttag cgagaaaggt ggacccctgt
      181 ttgtctgaaa cgtattcttt tgatgaaatt ggctatgccc atcaactgat gcatgaaaac
      241 aaacacccct acggaaacat ggcctgctta gtaaatgcta cagaaaaagg ccagggcaaa
      301 acagaggact aacgccaaac ccagtggcca aacaagagga tttgttaaag caagggctgc
      361 aacaaatagg gctcaacaca gaacaacaga atatcgacct attaatcgcg tacattaatt
      421 tgttgttaga gcaaaaccga acgcttaacc taaccgcaat cattgaccca ttagaagcag
      481 tcaccaaaca cctgctggac tctttaagca tagcgcaaga catctcagag ggcacagttg
      541 tagatgttgg gagcggcggt ggttgtccag gcataccgct ggcaatcatt aaaccagaaa
      601 gccaatttgt tctcgtggag tcaaaaaaca aaaaagccaa ttttttaaaa acaacgaccc
      661 aaacgctcaa aattaaaaac ataaaggttc tttgtcaaag agcagagaac attaagctaa
      721 caaaaaaagc ggacaccata gtttcaagag cgttgggcgg tcttccttac tttgtttcag
      781 tggcgggcca catgctaaaa aaacaaggaa ggctgcttgc aatgaagggg aaaatccctc
      841 actctgagtt aaaggagatt ccattagggt gggaggtcgt aaaaacaaaa aaaattgaag
      901 taccctacct gcaagcagag cgacacgtat taataatagc tagacaaatg taacatggcc
      961 aaaaccatcg caattgcaaa ccaaaaagga ggagtaggga aaaccactac agccgttaat
     1021 ttagcagccg cccttggcag catgggcaaa aaaacacttt tggtcgacct tgatccacaa
     1081 agcaatgcaa caagcgcatg cggcgttgag aaaaataagt taaacagctc tatttatgat
     1141 ctttttgtcg gagagggagc tgtagaagca accatattat tttcagaaaa aaacgggttt
     1201 tggatcttgc ctgcaaataa agatttactg gctgctgaaa tccaactact ggagcagcac
     1261 gagagagagt ttcagcttaa aaaaatacta gaaaaagcca ctgataattt tgattatatt
     1321 ttaattgatg ctccaccctc aatgaacata ttaaccctga atgcactagc cgcagctgga
     1381 gcggtgatca tcccagtcca gtgtgaatac tacgcactag aggggctgac cggcctgctg
     1441 gaaacaatca accaagtcaa acaaaagata aacccaagcc ttgaaatcaa aggggtttta
     1501 agaactatgt ttgacgttag aaacaacatc tcagtggagg tttctgtaca actaaaaaaa
     1561 caccttggga ataaattgct ttggacctct ataccaagaa acgtcaggct ggctgaagct
     1621 cccagctttg ggctctctgc attggcttat gatcaagggt gcttgggggc aaaggcgtac
     1681 ctttctttgg ctaaagaaat aacttaaaaa atcatggaat gattttttta actcgataaa
     1741 ctaaaacgta atatggacga aaaaaagaaa agcctaggac gaggactgga ggctttactt
     1801 ggggcctctg agcccgccca acaaaccatt aagccagacg aagctattca ccaattatct
     1861 attgaaaaaa taggaccagg accctttcag ccaagacgaa caattagaga agaacagctg
     1921 aaggaattag cactatccat tgaagagcaa ggaattattc aaccgattgt ggtcagggag
     1981 cgagcggttg ttgactcaca aacaggtgtt cgttttgaaa tcatagcagg agagcggagg
     2041 tggagagccg cacagctggc caaccttggt gccgtgccgg ccgtcattag aaccatgtca
     2101 gacgcatcag cggttgcagt agcgttgatt gaaaacattc agcgcgaaaa cctcaaccct
     2161 ttagaggaag caaacgcgtt tcagaagctg attatagaat acgaaatgac ccatcaagaa
     2221 gttgccaacg ctgtaggaag agccagagcc agcatcacca acaccttaag actgctggat
     2281 ttaagctcca gcgttcaagc actcttaaac gaagcaaaaa taaacatggg ccatgctaga
     2341 accctgttgt ctttaactga ttcctccatg cagttagagg tggcaaacct cattattgaa
     2401 aaaaaacttt ctgtcagaga cacagaaagt ttagttcgga gcatccttga aggcgcgaac
     2461 aaaccaaaaa caaaaaaaca acccgaagac cctgacatta aaagactcga aagcagccta
     2521 acccagaaac tgggtgcaaa agttttcatc aaacacgcaa aatctggcaa gggcaggtta
     2581 gtgattgcat acaatagctc agatgagctt caggggattt tagatagaat tcgatagagc
     2641 aagaaaacca ataactaaaa ccaagtgaca gcacaatcaa gaaaaatcgc gattggaatt
     2701 ctttctgcac aattggtttt agggatggtc acagcgggaa tattttggtt ttttttgagc
     2761 gttgagagcg caaaagcatc atttgctggc ag
//
LOCUS       DASCTH010000320         6516 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000174144, whole genome shotgun sequence.
ACCESSION   DASCTH010000320 DASCTH010000000
VERSION     DASCTH010000320.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000174144"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="QGH35_06675"
     CDS             complement(<1..671)
                     /locus_tag="QGH35_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012706.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJL82245.1"
                     /translation="MRTKQLVLILVFLVSGCGENQEIGCFVSDGAIVVGGQTGQELSP
                     AQVFRKNNGTEPGTLDPHRAEGVPASNVLRDLFEGLVMEDPSGAYIPGAAESWNISED
                     AKTYTFKIRENSKWSNGDVLTAEDFVYGLRRSADPATLSNYASMLYPIKNARDVVLGK
                     KKPQALGVSTDGPTTLIIELEEPTPYFLSLLTHSTTYAAHRPTVEEFGSQFTRPGNLV
                     SNGAF"
     gene            complement(668..2572)
                     /gene="mnmG"
                     /locus_tag="QGH35_06680"
     CDS             complement(668..2572)
                     /gene="mnmG"
                     /locus_tag="QGH35_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020394081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HJL82246.1"
                     /translation="MKTNNNKFDAIVVGGGHAGTEAAAALSRMGHKALLLTQSKETIG
                     QMSCNPAIGGVGKGHLVKEIDALGGVMALAADHAGIHFKTLNASKGRAVQATRAQADR
                     TLYKKAIQDHLNSLPRLSIVEGMAARLIVEANRVLGVETEEDQSFYAKHVILTVGTFL
                     NGKIYIGNKRTKGGRVGDEPSIMLADQLREIAPRTGRLKTGTPPRLLKKSINFSVLEE
                     QAGDNPRPIFSFLSSREDQPKQVSCFIAYTNKNTHSIIKKALSSSPLFTGAIEGVGPR
                     YCPSIEDKVTKFIDKTRHQIFIEPEGLFSEEVYPNGISTSLSLKNQESFVRSIVGFEN
                     AVIAKPGYAIEYDYFDPRDLKSTLETSAVKGLFFAGQINGTTGYEEAAAQGILAGINA
                     GLSIEEKEKWVPDRSEAYIGVLTNDLVTRGTNEPYRMFTSRAEHRLLLREDNADTRLT
                     PKAKELSLIAEKRWKIFCEKQEIIRKEKTRLSKTTLSGGAVQRIDNKSKRERGEKTAY
                     QLLKRPHVLYKNLLKEINPRSGYENETELLRETVDSIIEADAKYQGYMRRQHNEIEKQ
                     KAYKNTALPKDIDYQGLSGLSNEAKQKLIEFTPETIGQAARIQGITHASLSVLLVHLK
                     KHKLKKAEGL"
     gene            complement(2574..3917)
                     /gene="mnmE"
                     /locus_tag="QGH35_06685"
     CDS             complement(2574..3917)
                     /gene="mnmE"
                     /locus_tag="QGH35_06685"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HJL82247.1"
                     /translation="MNKKINKQADTIAACATAAGSSGLALIRVSGPNTAMIAKKIIGP
                     VPKPMRAKYSNFLSPDRSIIDSGIALYFKGPRSYTGENTLELTCHGGSAVVDQILQAL
                     IEYGARLAGPGEFTKRAFLNDKMDLTQAEAVVDLIESSSAATARAAKRSLDGVFSTKI
                     LNLSTLLSEIRVSVEASLDFPEEDIKETGQQDGLTFKMNETKDIFSTLLRQTRLACRV
                     REGVVVVLTGKPNVGKSTLMNTLCGHERAIVSESPGTTRDTLDEDILVDGYKVRLIDT
                     AGIREPENTIEKEGVNRAIKAAQNADIVINVVDGSTSQTTKKKNKTNDIVALNKNDLT
                     ENAPGRVENEANMFRLSALTGEGVDELLNHISNYFNQELLAEETLTARRRHLSALKEA
                     NKHFLEAEVKISAGRTTDLVAEDLLQAQNSLSEITGEFTSEDLLEKIFNEFCIGK"
     gene            complement(3920..5617)
                     /gene="yidC"
                     /locus_tag="QGH35_06690"
     CDS             complement(3920..5617)
                     /gene="yidC"
                     /locus_tag="QGH35_06690"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HJL82248.1"
                     /translation="MENLRLPLWAGFFVVAWFLISQWSADYAIKTDQPALPTDQTRQN
                     IPKEQTNKTALELSNSLPALSSELSSGSSVVQETKQKLDLVVVTTDVMEISINTKKGA
                     DIVQAKLLNYYPDKKNKTQNIELLSYGENSYHYLQTGLLSLNGQPEPNHQEAFVSNGT
                     KSSLKTNHAFLRDQQEDLKVSFVWQPQEENTNIEVVKSYIFKRGRYDVQLVYKIKNKG
                     SKDYPFVLYNRLVKKDIQTDRSMFDVETYSFNGPIIYNGDSYEKKETESLEGPAVETH
                     TSGWAANIQHHFLVAAIPQQGEPFTYRVDADKPNNLYRVSMVGEQPSALQPGRTTTAT
                     TTFFIGPKVQKQLRETAPGLELAVDYGRLTFLAAPLFWLLEKAHGIVKNWGLAIIFVT
                     FLIKLVFYPLTEASGKSMAKMRTVQPRLKTLQERYKDDKQALSQATMELYKKEKVNPL
                     AGCLPMLIQIPFFISFYWVLLESVEIRQAPFFLWIQDLSSRDPYFILPILMGLGMFFQ
                     QKLNPTPPDPMQAKIMMAMPIMFTGMFAFFPSGLVLYWLTNSLLSIAQQWRINRANT"
     gene            complement(5604..5951)
                     /gene="rnpA"
                     /locus_tag="QGH35_06695"
     CDS             complement(5604..5951)
                     /gene="rnpA"
                     /locus_tag="QGH35_06695"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HJL82249.1"
                     /translation="MTNKLNFNHVHSSNMRSEDELFSVFFVKNKTNRCRLGVSLPKKT
                     IAKAATRNKIKRIIKNSFNVFFQTTKGIDVVVRAKREPQTNTSDKILQSLDQHWKTIM
                     SQHNKTNKTNGKP"
     gene            complement(5962..6096)
                     /gene="rpmH"
                     /locus_tag="QGH35_06700"
     CDS             complement(5962..6096)
                     /gene="rpmH"
                     /locus_tag="QGH35_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HJL82250.1"
                     /translation="MKRTYQPSRIKRKRTHGFRARMSTKAGRKIINRRRARGRTKLSV
                     "
     gene            6278..>6516
                     /locus_tag="QGH35_06705"
     CDS             6278..>6516
                     /locus_tag="QGH35_06705"
                     /inference="COORDINATES: protein motif:HMM:NF023069.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HJL82251.1"
                     /translation="MQNTLWDHCLDTLKADLSEQNYNTWIKPLYVIEDEEGPSLTLLA
                     PNKFVVNWVQKNYLEQIKAAAEKKLQTTTTIAISVG"
BASE COUNT         1442 a         1440 c         1420 g         2214 t
ORIGIN      
        1 ttaaaagcac cgttgctcac caggttgccc ggccttgtaa attgggaacc aaactcctca
       61 acggttgggc ggtgtgctgc gtaggtggtt gagtgggtta gtaggcttaa aaaataaggc
      121 gttggctctt caagctctat gatcagggtt gttgggccgt cggtgcttac tcctagggcc
      181 tggggtttct tttttcctag cactacgtct ctagcgtttt ttattggata taacatgctt
      241 gcgtagtttg acagcgtggc cgggtcagcg ctcctgcgca aaccataaac aaagtcttca
      301 gccgtcaaga catctccgtt gctccattta ctattttcgc gaatcttaaa ggtgtatgtt
      361 ttcgcgtcct cactaatgtt ccaggattct gctgccccag gaatgtatgc tcctgatgga
      421 tcttccataa ccaagccttc gaacaagtct ctcaatacgt ttgaggcggg cacgccttct
      481 gctctgtggg ggtccagggt tccgggctct gtgccgttgt tttttctaaa tacttgagct
      541 ggtgaaagct cttgtccagt ttggccgcca acaacgattg cgccatcact aacaaaacat
      601 ccaatttctt ggttctcccc acagccagaa accaggaaaa ccagaatcaa aaccagttgc
      661 tttgttctca taggccttct gctttcttta gtttgtgctt ttttaaatgc accagcaaaa
      721 cggaaagcga ggcgtgtgtg atcccttgga ttctggccgc ctgcccaatg gtttctggtg
      781 taaactcgat gagtttttgt ttagcctcgt tggagagtcc tgagaggcct tggtaatcaa
      841 tgtcctttgg aagtgctgtg tttttatatg ctttttgttt ttctatttcg ttgtgttgtc
      901 tgcgcatata cccttggtat tttgcgtcgg cttcaattat ggagtctacc gtttctctca
      961 acagctctgt ttcgttttcg taaccgctcc tggggttaat ttctttcaac aggtttttgt
     1021 ataaaacatg gggtctcttt aatagctgat aggctgtttt ttcacccctt tctctttttg
     1081 atttattgtc gattctttgg actgccccac ctgaaagcgt ggttttggaa agccttgtct
     1141 tttctttacg gattatttct tgtttttcac aaaagatttt ccaccttttc tcagcaatga
     1201 ggctcaactc tttggctttt ggtgtcagtc ttgtgtcagc gttgtcctct ctcagtagta
     1261 gcctgtgctc tgccctgctt gtaaacattc tatacggctc gttggttcct ctggtgacca
     1321 ggtcgtttgt caacacacca atataggctt cgcttctgtc tggaacccac ttttcctttt
     1381 cttctataga caaacctgcg ttgattcctg ctaaaatccc ctgtgccgct gcctcctcgt
     1441 aaccagtggt gccatttatc tggcctgcaa aaaacaggcc cttgactgca ctggtctcaa
     1501 gcgttgattt gaggtctctt gggtcaaaat aatcatattc aatggcgtag cctggtttgg
     1561 caataacggc gttttcaaag cccactatag atctaacaaa gctttcttgg ttttttaggc
     1621 ttagacttgt agagattccg tttgggtaca cctcttccga aaacaggccc tctggctcga
     1681 taaaaatttg atgtctggtc ttgtctataa acttggtgac cttgtcctca atagaggggc
     1741 aatatcttgg cccaacccct tctatagcgc ctgtaaacaa aggagaggag ctgagtgctt
     1801 ttttgatgat cgagtgtgtg tttttattgg tgtatgcaat aaaacagctg acttgtttgg
     1861 gttggtcttc tcggctcgaa agaaaagaaa aaataggtct tgggttgtcg cctgcttgtt
     1921 cttccagaac agaaaagttg atactctttt ttaaaagcct aggcggagtg cctgttttta
     1981 gcctgcctgt tctgggtgct atttctctta gttggtctgc cagcattatt gacggctcat
     2041 cacccactct gccgcccttt gttcgtttgt tcccaatgta gatctttccg tttaaaaagg
     2101 tgcccacggt taaaattacg tgctttgcat aaaagctttg atcttcctcg gtttccacac
     2161 ccaaaaccct gttggcttca acaataagcc ttgccgccat tccctcaacg atggaaagcc
     2221 tgggcaagct gtttaagtgg tcttgaatgg cttttttata gagggttctg tctgcttggg
     2281 cgcgagttgc ctgaacggcc ctgcctttgc tggcgtttag ggttttaaag tgtattcctg
     2341 cgtgatccgc ggcgagcgcc attacaccgc caagggcgtc tatttctttt acgaggtgtc
     2401 cctttccaac accccctatc gctggattgc agctcatttg gccaatggtt tctttgctct
     2461 gggttagcag tagggctttg tgtcccattc ttgaaagcgc agcagcagct tcggtgccag
     2521 catggccccc accaacaact atcgcatcaa atttattgtt gtttgttttc agcttatttt
     2581 cctatgcaaa actcattaaa tattttttct agcaggtcct cgcttgtaaa ctctcctgta
     2641 atctctgaaa gagaattttg tgcttggagc aggtcctctg caaccaagtc ggttgttctg
     2701 ccagctgata ttttaacttc tgcttctaaa aaatgcttgt ttgcttcctt taaggcgctt
     2761 agatgtcttc ttcttgctgt tagggtctct tctgccaata gctcttggtt aaaataattg
     2821 cttatgtggt taagaagctc gtcaacccct tctccggtta gcgctgaaag acgaaacatg
     2881 ttggcttcgt tttctaccct gcccggagcg ttttcggtaa gatcgttttt gtttaaagct
     2941 acaatgtcgt tggtcttgtt ttttttcttt gttgtctggc ttgtgctgcc atcaaccaca
     3001 ttgattacta tgtctgcgtt ttgtgcggct tttatcgctc tattaacgcc ctctttttca
     3061 atggtgtttt ctggctctct tattcccgca gtgtctatta gtcgaacctt atagccgtca
     3121 accagaatgt cttcgtctag agtgtctctt gtggttcctg gggattcact cacgatcgct
     3181 ctttcgtgtc cacacagggt gttcatcagg gttgatttac caacattggg ctttcctgtt
     3241 agaacaacaa cgaccccctc tctgactcga caagctagcc ttgtttgcct aagaagtgtt
     3301 gaaaaaatat cttttgtctc gttcatttta aaggtgagtc cgtcttgttg ccctgtctct
     3361 ttaatatctt cttctggaaa gtctaatgag gcttcaacgc tcactctgat ctccgaaaga
     3421 agcgtgctta gatttagtat ttttgttgaa aagaccccgt ccagcgacct tttggccgcc
     3481 cttgctgttg ctgctgatga gctttctata agatcaacaa cggcctctgc ttgggtaagg
     3541 tccatcttgt cgttcaaaaa agctcttttt gtaaactccc ccgggccagc gagccttgct
     3601 ccatattcaa taagagcttg aaggatttga tcaactactg cgcttcctcc atgacaggtt
     3661 agctcgagcg tgttttctcc tgtatatgac cttgggccct taaaatatag tgcgataccg
     3721 ctatcaataa tggagcgatc tgggcttaaa aaattactat attttgccct catgggcttt
     3781 ggaacagggc ctattatttt ttttgctatc atcgctgtgt tgggtcctga aaccctaatc
     3841 aacgcaaggc ctgagcttcc cgcagcagtc gcacacgctg cgattgtatc tgcttgcttg
     3901 ttaatctttt tgttcaagat tatgtattag ccctgtttat tctccattgc tgtgcgatag
     3961 ataaaagcga gtttgttagc cagtagagca ctagccctga gggaaaaaaa gcaaacattc
     4021 cagtaaacat tattggcatc gccatcatga tcttggcttg cattgggtct ggtggcgttg
     4081 ggttaagctt ttgctggaaa aacatcccga gccccatcag tataggcaaa atgaagtagg
     4141 ggtctctaga agaaagatcc tggatccaaa gaaaaaaggg cgcctgccta atctcaacgc
     4201 tctccagcaa aacccaataa aacgatataa aaaatggaat ttgtatcagc atcggtaaac
     4261 agcccgccag cgggtttact ttttcttttt tataaagctc catcgttgct tggcttagtg
     4321 cctgtttgtc gtctttgtac ctctcttgta gggtcttgag tcttggttgg accgttctca
     4381 tttttgccat agatttgccg ctggcctctg tgagcggata aaacacaagc ttgattaaaa
     4441 atgttacaaa aataattgcg agcccccagt tttttacaat tccgtgggct ttttcaagca
     4501 gccaaaacaa gggggcggct aaaaaagtaa gtcttccgta gtccaccgca agctctaggc
     4561 caggtgcggt ttctcttagt tgcttttgaa ccttgggccc aataaaaaaa gtggttgttg
     4621 cggttgttgt tcttccaggc tgtagggcgc tgggttgctc tcccaccatg gacacccgat
     4681 aaaggttgtt tggtttgtct gcatcaaccc tataagtaaa aggctctcct tgttgtggta
     4741 tggccgctac caaaaagtga tgttggatat tggctgccca cccgcttgtg tgggtttcaa
     4801 ctgcgggacc ctctaggctt tctgtttctt ttttttcgta gctgtctccg ttataaatta
     4861 ttggtccatt aaatgaatag gtttcaacat caaacatgct tctgtctgtt tggatatctt
     4921 tttttacaag cctgttataa agaacaaaag gatagtcttt agaacctttg ttttttattt
     4981 tataaacaag ctgaacgtcg tatcttcctc ttttaaagat gtagctttta actacctcta
     5041 tgtttgtgtt ttcttcctgg ggctgccaca caaaagaaac ctttaggtct tcttgctggt
     5101 ctcttaagaa ggcgtggttg gttttgagcg aggactttgt cccattagaa acaaaagcct
     5161 cttggtggtt gggctctggt tgtccgttca gagacagcag cccagtttga agatagtgat
     5221 agctgttttc cccataggac aaaagttcga tgttttgggt tttatttttt ttgtctggat
     5281 agtagttgag cagctttgcc tgaacgatgt ctgctccttt tttggtgttg atagaaatct
     5341 ccatcacgtc tgttgttaca acaaccaggt caagtttttg ttttgtttcc tggacaacgc
     5401 ttgatccgct gctcagctcg ctggacagag ccggcaaact gttagaaagc tctagcgcgg
     5461 ttttgttggt ttgttctttt ggaatgtttt gtcgcgtttg gtctgttggc agtgctggct
     5521 gatcagtttt tatagcatag tctgctgacc actggcttat gagaaaccac gccacaacaa
     5581 agaaaccagc ccagagcggt agcctaaggt tttccatttg ttttgttcgt tttgttgtgt
     5641 tggctcatta ttgttttcca gtgttggtcg aggctttgta atattttatc acttgtgttg
     5701 gtttggggct ctctctttgc tctgacaaca acatctattc cttttgttgt ttgaaaaaaa
     5761 acattaaagc tgttttttat tattcttttt attttgtttc tcgttgctgc tttggcgatt
     5821 gttttttttg gaagagacac tcctaagcga cagcggtttg ttttattttt aacaaaaaaa
     5881 acagagaaca gctcgtcttc tgacctcatg tttgaggaat gcacgtgatt aaagtttagt
     5941 ttgtttgtca accgcttgac tctaaactga gagctttgtt ctgccccttg ctctccttct
     6001 gttaattatt tttctacctg ctttagttga cattcgcgca cgaaagccgt gtgttctttt
     6061 tctttttatt ctagatggtt gatacgttct tttcatgact caccaaattt tattttaaaa
     6121 aacaacacta tagttgtgct gcgtttgggg tgaaagatta tgggaggggg gcttgaatgt
     6181 caatcaaaaa acaacctgtg gataactaca ataagaaggt atagtattat ttggctttcc
     6241 gacaaataac ttgaaccctt ctttttttat aaacacaatg caaaacacac tctgggacca
     6301 ctgtttagac accttaaagg cagacctctc agaacaaaac tacaacactt ggattaaacc
     6361 tctttatgtt attgaagacg aagaggggcc gtctcttacc cttcttgcgc ctaataagtt
     6421 tgtggtaaac tgggttcaaa aaaactactt agaacaaatc aaggcggccg cggaaaagaa
     6481 gcttcaaaca acaacaacta ttgctatttc tgtggg
//
LOCUS       DASCTH010000321         1948 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000362395, whole genome shotgun sequence.
ACCESSION   DASCTH010000321 DASCTH010000000
VERSION     DASCTH010000321.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000362395"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="QGH35_06710"
     CDS             <1..413
                     /locus_tag="QGH35_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016916126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HJL82252.1"
                     /translation="PLLISSIVLFYLGALFAYWVVFPLMFAFFTSAAPTGVSVMTDIA
                     SYLEFVLVLFFAFGLAFEVPIATVVLVWSGLTTIETLAKNRAYVFLGTFVIGMLITPP
                     DVISQTLLAVPVYLLYECGILLSKLLTKNKPPDE"
     gene            406..1905
                     /locus_tag="QGH35_06715"
     CDS             406..1905
                     /locus_tag="QGH35_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HJL82253.1"
                     /translation="MNDATPSADYLDKGFLGHPIGLRTLFLTEMWERMSFYGMRGLLV
                     LFMTAEVFNGGFAISQSEANTIVAIYVGMVYFMSLPGGWIADRLLGKQKAVLYGGIII
                     ALGHYCLAVPMKETFYIGLSLVVLGTGLLKPNISAIVGELYTRADPRRDAGFTIFYMA
                     INIGSFLGFLICGYLGENVNWHYGFGAAGIGMTFGVAQYILTLKNLGGAGTEIQEKDP
                     NKRKQQLTFVLVIVVALFAFVLAGIFGLWALDAILLNRILALIIGLISIIYFVFIYFF
                     GKLTPLEKKNMVMIVFLFLGAAAFWAGFDQSAGSFNNFAKYNTNLSFFGLFEMPASWV
                     QLINPTFVVLFAPFFAALWLRLGAKNMEPGAPVKFAFGVILNGFGFLIMVYAVYAAVE
                     AADGRVGVQWLLLVYLFQTLGELSLSPVGLSAMSKYSPKRYSGQMMGVWFLASAFGGM
                     IAGQIGFEAASEGLGFMDDVFLNTFYALAGLGVVLIVASRFIKGEILED"
BASE COUNT          411 a          365 c          469 g          703 t
ORIGIN      
        1 ttcctttgtt gatttcaagc attgttttgt tctaccttgg agcgctattt gcttattggg
       61 tagtttttcc actcatgttt gcttttttca cttccgctgc ccccactggg gtctcggtta
      121 tgacagatat tgctagctac cttgaatttg tgttggtttt gttttttgct tttggtttag
      181 cttttgaggt tccaattgcc acggttgtat tggtgtggtc aggactcact accattgaaa
      241 cgttggcaaa aaatagagcc tatgtgtttc ttggcacttt tgttattggg atgctcatca
      301 ctccgcctga tgttatttct caaactttgt tagcggttcc tgtttacttg ttgtatgaat
      361 gtggcattct gttgtctaaa ttattaacca agaacaaacc acctgatgaa tgacgcaact
      421 ccttcagctg actacctaga caaagggttt ctcggtcacc caataggctt gagaacgctt
      481 tttctgactg aaatgtggga gcggatgtct ttttatggca tgcgtggctt attggttctg
      541 tttatgaccg cagaggtttt taatggcggt tttgcaatat ctcaatcaga ggccaacacc
      601 attgtggcta tctatgtggg catggtctac tttatgtctt tacccggtgg ttggattgcc
      661 gatcgcctac ttggaaaaca aaaagcagtt ttatatggag gcataatcat tgcgcttggc
      721 cattattgtc tggccgtgcc catgaaagaa accttctata tcggactttc tctggtggtt
      781 cttggaacag gtttattaaa gcctaatatc tcagcaatcg ttggagagct ctatactaga
      841 gcagatccta gaagagacgc tgggttcact attttttata tggcgatcaa cattgggagc
      901 tttcttggtt ttttaatctg cggctactta ggcgagaatg tcaactggca ttatgggttt
      961 ggtgcggccg gtattggtat gacttttggc gtggcccagt atattttaac gcttaagaac
     1021 cttgggggag ccggaactga gatccaagaa aaagacccta ataaaagaaa acaacaactc
     1081 acttttgtgt tggttattgt tgttgctctg tttgcctttg tcctagctgg gatttttgga
     1141 ctctgggctc tggatgctat tttactcaat agaattctgg cattaataat aggcctgatc
     1201 tctattattt actttgtttt catttatttt tttggaaagc taacccctct agaaaaaaag
     1261 aacatggtga tgattgtttt tctttttttg ggagcggcag ctttttgggc tggctttgac
     1321 cagagtgctg gttcgtttaa taattttgca aaatataaca ccaatctttc tttctttggt
     1381 ttgttcgaaa tgcctgcttc ttgggtgcag ttgattaatc cgacctttgt tgttcttttt
     1441 gctccttttt ttgcagcgct ttggcttcgg cttggggcta aaaacatgga gcccggcgcg
     1501 ccggttaaat ttgcctttgg ggtcatccta aacggctttg gttttctgat catggtctat
     1561 gctgtttatg cagcagtgga ggctgctgat gggagggttg gtgttcagtg gttgttgctt
     1621 gtttacctgt tccaaacgct gggcgagctg tccctcagtc ctgtggggtt gtcagctatg
     1681 tccaaatatt cacccaaaag atattcaggt caaatgatgg gagtttggtt tttggcctct
     1741 gcgtttgggg gcatgattgc agggcagatt ggttttgagg ctgcctcgga gggcctcggt
     1801 tttatggacg atgtcttttt gaacactttt tatgctcttg cagggctcgg tgttgtgttg
     1861 attgtggctt ctcggtttat taagggagaa atcttagaag actagccaaa aatcttttta
     1921 gtctcctgaa acaaaactta accattaa
//
LOCUS       DASCTH010000322         3154 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000766697, whole genome shotgun sequence.
ACCESSION   DASCTH010000322 DASCTH010000000
VERSION     DASCTH010000322.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000766697"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="QGH35_06720"
     CDS             complement(<1..247)
                     /locus_tag="QGH35_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82254.1"
                     /translation="MEKKVIDSDVWRSFAINPNPDFTPPRWDLTIDRNILVELLHHCY
                     KSFYLRPGYIGKQLLKIRSFKEFNSKTTAGFSLLYNQV"
     gene            490..1989
                     /gene="istA"
                     /locus_tag="QGH35_06725"
     CDS             490..1989
                     /gene="istA"
                     /locus_tag="QGH35_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HJL82255.1"
                     /translation="MYPVGLYRQVRQAYYIHNKSIRDISRTHGLHRDTIRKMLLYSIP
                     PGYQRSKPIRKPKLDPFIGIIDSYLKDDKNRPRKQRHTAKRIFERIQDEHGYEGGYTI
                     VKDYVREKRFRCREMFVPLSHPPGHGQVDFGEALAIISGVELKAHYFCIDLPQSDNLF
                     VKAYPAETMEAFCDGHNSAFSCFGGVPQSILYDNTKIAVARIEKGGKRRLTRKFEELL
                     SHYLYEARFGRPGKGNDKGKVEGVVGFARRNFLVPIPRFDSFDSLNKYLEEQCRKRRD
                     RQLRSHKETIGERFERDKQAFLPLPAFPYDACDKINTRVNSLSLVRYKTNDYSVPVAY
                     GHREVQVSAYVHEVIISCGTEKIARHERSYDREDFIYDPLHYLPLIEQKINSLDQAAP
                     LQKWELPDEFATLRRLMESRMGKKGKREYVQVLRLIETFRLEDVLTGMREAMRLKAIS
                     FDAVKHLLLCHIEERPARLDLECYPYLPKAQVQTTLARQYMELLQGVQP"
     gene            1986..2747
                     /gene="istB"
                     /locus_tag="QGH35_06730"
     CDS             1986..2747
                     /gene="istB"
                     /locus_tag="QGH35_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HJL82256.1"
                     /translation="MSEKPQLLLAHYLKTLKLPTFYREYGKVAKQCSVEGVDYEGYLL
                     RLSEQELIDRQRRMVERKIKQAKFPTIKRLDSFDFKAIPSLNKRLVMELARCEFIERK
                     ENVIALGNSGTGKTHIATGLGVAACQKGLSVFFATAASIVNELLEARDEKRLLRFQKY
                     LFRHKLLIIDELGFVPLSKTGAELLFEVFSQRYEQGSVLVTSNLPFDEWTEIFASERL
                     TGALLDRITHHVHILEVNGESYRLKQSKKKEKKVK"
BASE COUNT          956 a          635 c          682 g          881 t
ORIGIN      
        1 ctacctggtt atataataaa ctgaaacctg ctgtcgtttt tgaattgaat tccttgaagc
       61 ttctaatttt taaaagttgt tttccaatat accccggtct caaataaaaa cttttatagc
      121 agtgatgaag caattcaacc aaaatatttc tatcgattgt caaatcccac ctcgggggag
      181 tgaagtccgg attagggttg atggcaaagc tcctccacac atccgagtcg attacctttt
      241 tttccattgc ctcatagtat aaaggtgtgc cgggatagag ggttgtaatg gtaaattgag
      301 caaaatccgc gtcaagcttt ttagcaaaat caattgactg gtagatatca tcaattgttt
      361 ctgagaacgg cggtgtaata atgtcccact gagtcgtaaa ttttccaaga tatggcggct
      421 aaaaagtgta ccgcctgcta agctttcttt cagtcaataa attcataata ctgaaaggag
      481 gcggaaagga tgtacccagt gggattatac agacaagtta gacaagcgta ttatattcat
      541 aataaaagta ttcgtgatat ttcaaggaca cacggtcttc accgagacac aatccgcaag
      601 atgctgctgt attcaattcc tccggggtac cagcgaagta aacctataag gaagcccaaa
      661 ctcgatccat ttataggtat tatcgactcc tatctcaaag atgataaaaa ccggcccagg
      721 aagcaacgcc atactgccaa gaggatattc gagcgtattc aggacgagca tggatacgaa
      781 ggcggttaca cgatagtaaa agattatgta cgagaaaaac gttttcgttg ccgcgagatg
      841 tttgttcctt taagccatcc tcctggacac ggtcaggtgg attttggtga ggcccttgcg
      901 attattagcg gggttgagtt gaaggcgcac tatttttgta ttgatcttcc tcagagcgac
      961 aatttatttg tgaaagcgta tccggcagag acaatggaag ccttctgcga tgggcataac
     1021 tctgcttttt cgtgctttgg cggtgtgccg cagagcatat tgtacgataa cacaaagatt
     1081 gcagtagcca ggatagaaaa aggcgggaaa cgcagactta cccggaagtt tgaggagttg
     1141 ttatcccatt atctttatga agcccgtttt ggtcgtcccg gcaagggcaa cgacaagggt
     1201 aaggtagaag gagttgttgg ctttgcccga cgtaactttc ttgttcctat tccgaggttc
     1261 gacagttttg actcactaaa caagtatctt gaggaacaat gccgcaaacg acgagaccga
     1321 cagcttcgta gtcacaaaga gacaattggc gagcgttttg aacgtgacaa gcaagccttc
     1381 ctaccgctac cagcttttcc ctatgatgca tgcgataaga taaatacaag agtcaactct
     1441 ctgtcactgg tgcgatacaa aaccaatgat tattctgtac ctgtcgctta tggccacagg
     1501 gaggtacagg ttagcgctta tgtccacgaa gttatcataa gttgcggaac agagaaaata
     1561 gccagacatg aacgctccta tgatcgagaa gattttatct atgacccctt gcattatcta
     1621 ccgctcattg aacagaagat aaactctttg gatcaagcag ccccattgca aaaatgggaa
     1681 ttgcctgatg agtttgccac attacgccgg ttgatggaat cccgtatggg taaaaagggc
     1741 aaacgagaat atgttcaggt attaagactt attgagacgt tcagacttga agatgttcta
     1801 actggtatgc gggaagccat gcggcttaaa gcaatctctt ttgatgccgt caaacacctt
     1861 cttttgtgtc atattgaaga acgaccagca cggcttgatc ttgaatgcta tccctatctt
     1921 cctaaggctc aagtgcaaac aacgttagcc aggcagtata tggaactttt acagggagta
     1981 cagccatgag tgaaaaacca cagcttttgc tggctcatta tctgaaaaca ctcaagctac
     2041 caacgtttta tcgtgaatat ggcaaagtgg ccaagcaatg cagtgttgag ggagtggatt
     2101 atgaaggata tttattacga ctttctgaac aggagttaat cgaccgacaa cgcagaatgg
     2161 ttgaacgtaa gatcaaacaa gccaagtttc ctaccatcaa aagactcgat agttttgatt
     2221 ttaaagctat accctccctt aacaaaagat tggttatgga actggcccga tgcgaattta
     2281 ttgagaggaa agaaaatgta atcgctcttg gaaatagcgg tacaggtaaa acacatatag
     2341 caacaggtct tggtgtagcc gcttgccaga aaggattatc tgtgtttttt gcaactgcgg
     2401 cctctatagt aaacgagtta ctggaagctc gggatgaaaa acgtcttttg cggtttcaga
     2461 aatatttatt caggcacaaa ctacttatta ttgatgagct tggattcgtg ccattgtcaa
     2521 aaaccggagc agagctgctc tttgaggtat ttagccagag gtatgaacaa ggttcagttc
     2581 tggttaccag caatctcccc tttgatgaat ggacagaaat ttttgcttca gaaaggctta
     2641 cgggagctct cctcgatagg atcactcacc acgtccatat ccttgaggta aacggtgaga
     2701 gttatcgact aaaacaaagc aaaaaaaaag aaaaaaaagt gaaataacaa aaaaacttca
     2761 taaggggtac ccccttatga actatacctt taactacatt ataggtggta cacttttacg
     2821 ccgccaagtg gtacattttt actccggcct tgacacacac ggaactttac tcctaattca
     2881 tccttagcga tacattcgca ttctttgata tctattttca gaattttagt tttatatcta
     2941 ttgtgcataa ttcactcatt taaataaata gtggatgatt taaggataac atcaattatg
     3001 gaattactaa aattgaagtg ttatctttat acttccccac tttcgctttc tttttttcaa
     3061 ctcaaactgt tatcataaac tacaaagagg agaaggagta tggatgtgga gattcccata
     3121 cacagatcgg cagattgacc cagccccctg agat
//
LOCUS       DASCTH010000323         1576 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000138327, whole genome shotgun sequence.
ACCESSION   DASCTH010000323 DASCTH010000000
VERSION     DASCTH010000323.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000138327"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="QGH35_06735"
     CDS             <1..289
                     /locus_tag="QGH35_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HJL82257.1"
                     /translation="EGARREVNEELGLGCQTTSLVGVYSFFQMNQVIIAYHVLLEEGE
                     INLDPAELVDHKTIPIDKLRPWPSGTGEAVRDWLSTQGIENKPIPFGKPKK"
     gene            complement(333..>1576)
                     /locus_tag="QGH35_06740"
     CDS             complement(333..>1576)
                     /locus_tag="QGH35_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HJL82258.1"
                     /translation="VPLEWPPYSGTYPMVSVPVVIGDTALAFNGGTNQMEPNMWTNAN
                     VTPSNPSGMGPTTRQEYLTFSVADNYIVPKMVLTYEASDDLMVYGSWTESKKPGGFSS
                     LGNGAFGFDPNGDGLPLEVQFDPEIMTVWELGFKSFWNEGALRINGAFFLEDYTDRQV
                     NVQKVIAGTLGSVTDNADGGEAFGFEVDIAWAISEQLTFTGGYTFLDTEYTDFKIYSR
                     SAGEIARDGTCVLIEDYLGVAGEQTCMIDKTGNEFERAPKNAAVFGLNYSDQLKNRDA
                     SFFVELNARFTDERWIDNSNDAYVDDAWRFDLNAGIEKGNWDVNFYVHNLLDDDTVLS
                     AGTGPDIGNSDFRFGLVLTLVETEAYPYGPVIYTQKDDAGQYGSGDSSLSIVPAPMIR
                     NMWYANIPDPRQVGMRVTYKF"
BASE COUNT          445 a          379 c          349 g          403 t
ORIGIN      
        1 agaaggggcg agaagggagg ttaatgagga actgggattg gggtgccaga ctacgagctt
       61 agtgggcgtg tatagttttt ttcaaatgaa tcaagtcatc attgcctatc atgttctttt
      121 agaagagggc gagataaact tggacccagc cgaattggtt gatcacaaaa ccattcccat
      181 agataaacta agaccctggc ctagtggcac cggagaagca gttcgagact ggctgagcac
      241 acagggtatt gaaaacaaac ccataccttt tggcaagccc aaaaaataaa cccctctgtg
      301 aagaggggtt tattgattca tagctttctg ttttagaact tataagttac acgcatacca
      361 acctgtcttg ggtcgggaat atttgcatac cacatatttc taatcattgg cgcaggtaca
      421 attgacagac tactgtcgcc tgagccgtat tgaccagcat catctttctg ggtgtaaatc
      481 accggcccat aaggataagc ttcagtttcc accagggtta ggaccagacc aaacctaaag
      541 tcggagttac caatatcagg ccctgttccg gctgaaagca cagtgtcatc atctaagaga
      601 ttatgcacat aaaaatttac atcccaattg cctttttcga taccagcgtt gagatcaaaa
      661 cgccaggcgt catctacata agcatcattg gaattatcga tccagcgctc atccgtaaag
      721 cgagcgttca actcgacaaa aaaggaggca tccctatttt tcaattgatc tgaatagttc
      781 agaccaaata ctgctgcatt tttgggagct cgctcaaact cgttgccagt cttgtcaatc
      841 atgcaggttt gttctccggc cactccaagg tagtcctcta taagaacaca tgtaccatcc
      901 cgtgcgattt caccagcact tctggaatag attttaaagt cggtgtattc ggtatcaagg
      961 aatgtatagc caccggtgaa agtcagttgc tcgctgatag cccaagcgat gtcaacctca
     1021 aagccgaaag cctcaccacc gtcagcatta tcggtaacac tacccagcgt gccagcaatg
     1081 actttttgaa cgttaacctg ccggtcggta tagtcttcta aaaagaacgc tccattgatc
     1141 ctaagcgctc cctcgttcca gaacgattta aaaccaagct cccataccgt cattatttct
     1201 gggtcaaatt gtacttcaag cggtagaccg tcaccgttgg ggtcaaagcc aaaggcacca
     1261 tttcccaagg atgaaaaccc accgggtttt ttggattcag tccaagagcc atagaccatg
     1321 aggtcatcac tggcttcata ggtaagaacc atcttgggca caatgtagtt atctgctaca
     1381 gaaaacgtca aatattcctg acgggttgtt gggcccattc ctgatgggtt gctaggtgtg
     1441 acattggcgt tcgtccacat gtttggctcc atttggttgg ttccgccgtt aaaagctagg
     1501 gcagtatcac caatgacaac aggcacagac accataggat aggttccact ataaggcggc
     1561 cactccagag gcacaa
//
LOCUS       DASCTH010000324         4301 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000615548, whole genome shotgun sequence.
ACCESSION   DASCTH010000324 DASCTH010000000
VERSION     DASCTH010000324.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000615548"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(60..1010)
                     /gene="rmuC"
                     /locus_tag="QGH35_06745"
     CDS             complement(60..1010)
                     /gene="rmuC"
                     /locus_tag="QGH35_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020030135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HJL82259.1"
                     /translation="MNEIILILLILLSCLVSFNIYLNLKKNDDENNEDEEILGEITES
                     LAQQKNVLQNQKEDIVKLTLGLSEFNYPLKQLTRYLSGGTLAGRFGEWGLEAIITDII
                     PSNKIKKNYEIKKGSDQRVEFAIDLDEGLLPIDAKFPSALYDTYVKAAEKQDQNAKKE
                     VDNALKAIKRSTKGNAKDINEKYMLKGVTIDFGVMFIPSESLMQLIDRLQEDTIERST
                     KEQIFRDYRVLIMGPNSFAAFLISLNMGFKSIALNERAEEILHKFGKLEKEFRQFEGM
                     TEELRDNAQKIIKAIDQGDVRVRAMNRVIKEMEELSEKKD"
     gene            complement(1086..>4301)
                     /locus_tag="QGH35_06750"
     CDS             complement(1086..>4301)
                     /locus_tag="QGH35_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012630.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007156 - homophilic cell adhesion via
                     plasma membrane adhesion molecules [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin domain-containing protein"
                     /protein_id="HJL82260.1"
                     /translation="FEENAEGVIIGTLSVIDPSFSGSITYTLSGTGSENFEVVDGQLK
                     LKDGISANYEVLNSYSVMITATDDSGYTKATTYAIEVIDVNEIPTAIALSATAIDENS
                     VGAIVGDLTTTDEDIGDSHTYTLTGDDADSFEVVDGQLKLKDGVSANYEAQNSYAVTV
                     TATDSGGLTTSQAFTVTINDVNDAPSAIKLTGSLYVNENDTGGIVGNISTVDEDTVDN
                     HTYAISGMHADSFEVVNGQLKLKDGINPVHQTQSSYSITVTSTDKGGITISEDYSVEV
                     NIAPTAFSLDSQSVDENYVSSPVGWIFVTDANINDDFTYILSGVGAENFEVVYGQLMV
                     KSGTWLDYETQSTYTLTIIVTDQGGYSLEKTLTIDVNDVDYGNPWVSEHVSVYDMPMS
                     DDIGIYGQQWFYNWEDGFQPLSLRFKNDDDPSTPLVITYSLMNANSVLGDNYDDGYEQ
                     GEDGVYSNIENYSAEWEAAVDSAFEYWGNVSGITFVKVDDNENMCGDIRVALSSGNFG
                     GAGGWSNVPYYYQGENNSAANDIWIRADYDQWDDDWPQYNLYILHHEIGHSLGLSHTH
                     HDYYSTVEQNTGLYSVMSYIGFGYLVNPWPGYEVDDNIIQDRPAINDIKTIQYLYGMT
                     PEFNQGNTTYTYTGPVYTTIYDTGGIDTIDVSSYDLDITLDLRGGMVSFIGTEELELE
                     VPYGNGSGDYSYEYSGFPIGISENTIIEHAATGSGNDTITCNEAGNIITCGEGDDDVF
                     NVGSGDTIYGGHGYDSFWITALDFTLIRGGSGTDVMNGEGDMLVFDNFADTSIDLRAF
                     TDAQLAGIEDIDIEDGRATTLKISYLSLMNLECTYLRDMDGDLDTDVVVYIHADALLD
                     EIQINDEGWTLTIPIDNGDNIFEGYNYYSSADGEVWFAFTAGTSVIINPDGNYQALIG
                     NQKATDNIDQPVTAPNDEDASITPPDGDCRPASNDNDQTQSSPGIVKGDDFGCRTRED
                     DLPNLWAEADNLGDLMLPETVAELDDLPKPDLSLLAGLVSDDTESWVMAFEPMAADNV
                     ISASIENIKAFESDPISQTDLIIDTDWNPIQEELLYISELG"
BASE COUNT         1187 a          983 c          825 g         1306 t
ORIGIN      
        1 atatatatca ataacttatg attttaagag tatggtaaaa acctcaaatt gagaagtact
       61 caatcctttt tttcgctcaa ttcttccatc tctttgatga ctctgttcat agcccttact
      121 cttacatcac cctgatcaat agcttttatt atcttttggg cattatctct gagttcttca
      181 gtcattccct cgaattgcct gaactctttc tctagctttc caaatttatg tagtatttcc
      241 tcggctcgtt cattcaatgc gatggattta aagcccatgt taagactaat caggaatgca
      301 gcaaaagagt ttggacccat aatcaacact cgataatctc tgaaaatctg ttctttagtg
      361 cttctttcta tagtgtcttc ttgaaggcga tcaatgagct gcatcagaga ttcactgggt
      421 atgaacatca ccccgaaatc aatggttaca cccttgagca tgtatttttc attaatatct
      481 ttggcattcc ctttagtact tcttttgatg gctttcagag cattgtccac ttcttttttg
      541 gcattctgat cctgcttctc tgctgccttt acataggtgt catagagagc cgatgggaat
      601 tttgcatcga ttggtaataa tccctcatcc aggtcaatgg cgaattcaac cctctgatca
      661 ctccctttct tgatctcata attcttcttg attttattgc taggaataat atctgtgata
      721 attgcctcta aaccccattc accaaatcta ccggctaatg taccgcctga tagataacga
      781 gtgagttgtt ttaagggata attaaattca gatagaccca gtgtcagttt tacaatgtct
      841 tccttctgat tttgaagaac attcttttgt tgggctaatg attccgttat ttcaccgaga
      901 atttcttcat cttcattgtt ttcatcatca tttttcttta ggtttaaata aatattaaag
      961 ctaactaaac aacttagaag aattaacagt attagtataa tttcattcat cgactacttt
     1021 cccagttttt agattatcaa tttaatcaaa atgataatag atactttatg atataaagtt
     1081 agagactacc ccagctcaga aatatataac aactcctcct gtatggggtt ccagtcggta
     1141 tcaatgatca gatccgtttg gctgatggga tcagactcaa aggctttgat gttctcaata
     1201 ctggcactga tgacattatc tgctgccatc ggttcaaagg ccatgaccca gctttccgtg
     1261 tcatcagaga ccaaacctgc aagtaatgac agatcaggtt tgggaagatc atccagttcg
     1321 gcaaccgtct cgggcaacat gagatcaccc agattgtcag cttcggccca gagattgggc
     1381 agatcatcct cacgggtccg acaaccaaaa tcatctccct tgacaatacc aggactagac
     1441 tgcgtttgat cattatcatt gcttgcaggt cggcagtctc cgtccggggg tgtgatagaa
     1501 gcatcttcat cgttaggtgc tgtgacgggt tggtcaatat tgtcagtagc cttttgatta
     1561 ccaattaatg cttgatagtt gccgtctggg ttgatgatca cactggtgcc ggcggtgaag
     1621 gcaaaccaga cttctccgtc tgccgatgaa tagtaattat agccttcaaa tatgttgtca
     1681 ccattatcga tcggaatggt caacgtccaa ccctcatcat taatctgaat ctcgtccaat
     1741 aaagcatcgg catgaatgta gaccacaaca tcagtatcca aatcaccatc catgtcacgg
     1801 aggtaggtgc attctaaatt catcagggat agataagaaa tttttaaggt agtcgctctt
     1861 ccatcttcaa tatcaatatc ctcgatgcct gctaactggg catcggtgaa agctcgaagg
     1921 tcaatgcttg tatcagcaaa gttatcgaac acaagcatgt cgccttcccc attcatgaca
     1981 tcagtaccac tgccacccct gatcagagta aaatctaagg cagtaatcca aaaagaatca
     2041 tacccatgtc cgccataaat ggtatctcct gaaccgacgt taaaaacatc atcgtctccc
     2101 tctccacagg tgatgatatt tcctgcctcg ttacaggtga tggtgtcatt gccgctgccg
     2161 gttgctgcat gttcaataat ggtattttct gaaatcccga tcgggaaacc agaatactca
     2221 taactataat caccactgcc attgccataa ggaacttcaa gctcaagttc ttctgtgcct
     2281 atgaatgaaa ccattccccc tctgagatcc aaagtaatat ccaggtcata actggaaaca
     2341 tcaatggtat caatgccacc ggtgtcataa atggtggtat aaaccggtcc cgtataggtg
     2401 taggtggtgt tgccctgatt aaactccgga gtcatgccgt agaggtactg gatggtttta
     2461 atgtcattga tcgcaggtcg atcttgaata atattatcgt ccacttcgta gccgggccaa
     2521 ggattgacaa gatatccaaa tccaatatag gacatcaccg aatacaggcc tgtattttgt
     2581 tctaccgtgg aatagtagtc atggtgagta tgtgataacc caagtgaatg cccaatttca
     2641 tgatgcaaga tatataaatt atattgtggc caatcgtcat cccattgatc atagtccgct
     2701 ctgatccaga tatcattggc tgcagaattg ttttcaccct ggtagtagta gggcacgttc
     2761 gaccagccac cagccccacc aaaattacca ctagataaag ctacacgaat atcaccacac
     2821 atattttcgt tgtcatcgac tttgacaaag gtaataccgg aaacattgcc ccagtattca
     2881 aaggctgaat caactgccgc ttcccattca gcagaataat tttctatgtt tgaataaacg
     2941 ccatcttcac cttgttcgta tccatcatcg tagttatcac ctaaaacgga atttgcattc
     3001 atcaaactgt aagtgatcac aagaggcgtg ctgggatcat catcattttt aaatcttagg
     3061 ctcaaaggct gaaacccatc ttcccaattg taaaaccatt gctgtccata tatcccaata
     3121 tcatcggaca tgggcatatc atatacactg acatgttcac tgacccatgg attgccataa
     3181 tcgacgtcgt tgacatcaat cgtcagggtt ttttctaaac tgtaaccacc ttggtccgta
     3241 acaatgattg tcagggtgta ggtgctttgt gtttcataat ccaaccaagt cccactcttg
     3301 accatgagtt gaccataaac cacctcaaag ttctcagcac cgaccccact taaaatgtaa
     3361 gtaaaatcat cattgatatt tgcatcagtc acaaaaatcc aacccactgg actgctaaca
     3421 taattttcat ccacagattg actgtcaagg ctaaatgctg tcggggcaat attgacctca
     3481 acagaatagt cctcactaat ggtgattcct cctttatcgg ttgaggtgac cgtgatgcta
     3541 taactgctct gggtttgatg aacaggattg atgccgtctt tgagtttcag ttggccattg
     3601 accacttcaa aagaatcggc atgcatgcct gaaatggcat aggtatgatt gtccacggtg
     3661 tcttcatcaa cagtcgagat attgccaacg atgccacctg tatcattttc attaacatac
     3721 agactacctg tgagtttaat tgcagaggga gcatcattaa catcgttgat ggtgacggtg
     3781 aaggcttgtg aggtggtcag tccgccacta tcagtggcag tgactgtgac agcgtaactg
     3841 ttttgggcct cataattagc actgactccg tctttgagtt tcagttgacc atcgaccact
     3901 tcgaaggaat cggcatcatc acctgtgagt gtataggtat gactatcacc tatatcttca
     3961 tccgtggtgg tcagatcccc gactattgcc cctacagagt tctcatcaat agcggtagca
     4021 gagagtgcaa tggcggttgg tatctcatta acatcaatga cttctatggc ataggttgtt
     4081 gcttttgtat agccactatc atcagtggcc gtgatcataa ccgagtaact gttgagtact
     4141 tcgtaattgg cactgatccc gtctttaagt ttcaattggc catcgaccac ttcgaagttc
     4201 tcagaaccgg tgccagataa ggtgtaagtg atactaccgc taaaacttgg atcaatgacc
     4261 gagagggtgc cgatgatgac accttcggca ttttcttcaa a
//
LOCUS       DASCTH010000325         2486 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000310136, whole genome shotgun sequence.
ACCESSION   DASCTH010000325 DASCTH010000000
VERSION     DASCTH010000325.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000310136"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="QGH35_06755"
     CDS             complement(<1..595)
                     /locus_tag="QGH35_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HJL82261.1"
                     /translation="MSIEADEVSPGVLRTPDDRFENLQDYPFEPHYMEIEDLRIHYLD
                     EGPKDADPIILFHGEPTWSYLFRKMIPVFTAAGHRVIVPDMVGFGKSDKLISKHDYSY
                     KHHVDVMKELVIRLDLKEATHFGQDWGGLVGLRVVAELPDRFARVVVSNTGMVARGGF
                     SAWLAQNFVKLVVWWHGPVTFEELKAASQETLNTENPS"
     gene            909..2042
                     /locus_tag="QGH35_06760"
     CDS             909..2042
                     /locus_tag="QGH35_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HJL82262.1"
                     /translation="MALNFGFSDSQELYREQIRRFARTELAPMRQKWDLERERPQAMV
                     KKALDAGLLDADMSHLMRGIMIEEVGYVDFNCALPFLVTTEPFELNRLDGVPDEIRDP
                     VRQAVREGKTIIAVGFTEPDGGADVANFRSTAVLDGDEWIINAVKNSISWANADYFTI
                     TCRTEDAAKGIWSLSNFFIPKKTKGVKSPRLWDDMGTHGAERGEVRFEDVRIPKDYLV
                     GERGKAYLQMAQLFDTNRAYIGLKCIGAAQASIDETCEFAKQRTVMGKPISGYQGVSF
                     PLVEAETQLEAARLLCYKTLWLRDEDLPNSKEGGMCKWWVPELTFEIVRQCLTLHGHY
                     GYSTELPFEQRLRDILGWQIGDGTSQISKLLVARSMFGKEFAG"
     gene            complement(2057..>2486)
                     /locus_tag="QGH35_06765"
     CDS             complement(2057..>2486)
                     /locus_tag="QGH35_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013044.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HJL82263.1"
                     /translation="GLNSRITFPFRRIEDLRNIAEEKRDITGKVTYVYSVFPNCTVAF
                     LSDHISVVISEPVSPTETQYFSYRLGQFSGNETEEDIERMRRDARFVTETGLEEDNAV
                     VAGIQDGLKSAANTHFIYGLYEKAIVHFLKNLTDLVNKAC"
BASE COUNT          693 a          559 c          528 g          706 t
ORIGIN      
        1 gagaaggatt ttcagtattt aatgtttctt gcgatgctgc ttttagctct tcaaaagtaa
       61 caggaccatg ccaccatacc actaacttca caaagttctg ggcaagccaa gcagaaaaac
      121 cacctctggc taccatccct gtattagaca caacaaccct tgcaaatcta tcaggcaatt
      181 cagctaccac tctcaaccca actaatcctc cccaatcttg cccaaaatgg gtagcttctt
      241 tcaagtccaa ccttattaca agttccttca tgacatctac atgatgtttg taactgtaat
      301 catgtttaga aattaatttg tctgattttc caaagccaac catatcaggc acgataactc
      361 gatgcccagc agcagtgaaa actgggatca ttttacggaa taaataactc caagtaggtt
      421 ctccatgaaa taggatgatg gggtcagcat ctttgggtcc ttcatcaaga taatggatcc
      481 ttaaatcctc tatttccatg taatgcggtt caaaaggata atcttgcaga ttttcaaaac
      541 gatcatccgg ggttcttaaa actccaggag agacttcatc tgcttctata gacattggag
      601 agaagagtgc cattaagcta aaaattacta aatatttcat attcttggat tttatcacta
      661 acctacaagt gattggtgta accaattgta acctctgctt tatgcatctt agtaatcctc
      721 ttcgagaatc cctttctctt ttagggtgtt tatcagtttt tctactcttt ttgtcaatat
      781 aagtacaaac gtaagtgctc caattcctat catactgaaa acaaatgcag taaccgttaa
      841 tccttccata attttctcca tacgataaaa gctgagatta tattctaaat aaaccttata
      901 gtgtattcat ggctctaaac tttggcttca gtgattctca agaactttac cgggaacaaa
      961 ttaggcgatt cgccagaact gagcttgcac ctatgcggca aaaatgggat ctggaacggg
     1021 aacgacccca agcaatggtc aaaaaggcgt tggatgctgg cctgcttgac gcggacatga
     1081 gccacctcat gcgtgggata atgattgaag aagtgggtta cgtagacttc aattgtgctt
     1141 taccgtttct tgttactaca gagcccttcg agcttaatcg gcttgacggt gtaccagatg
     1201 aaattcgcga tccggttcgc caggctgttc gtgaaggaaa gaccatcatt gcagtgggct
     1261 ttaccgaacc tgatggcggc gctgatgtag ccaattttcg tagcacggct gtgctggacg
     1321 gagatgaatg gataatcaac gcagtcaaaa actctatctc gtgggccaac gcggattact
     1381 ttaccatcac ctgtcgaacc gaagatgctg cgaaaggcat ctggtcactg agcaacttct
     1441 tcataccaaa gaagacaaaa ggcgttaagt ccccaaggct ctgggatgac atgggtactc
     1501 atggtgctga gcgaggggaa gtgcggtttg aggatgtacg gatcccgaaa gactatctgg
     1561 ttggggaaag aggtaaagca taccttcaga tggcacaact attcgatacc aaccgtgcct
     1621 acattggtct taagtgcatc ggtgcagctc aggcttccat cgatgaaaca tgtgaatttg
     1681 ctaaacaacg tactgttatg ggtaaaccca tttcgggtta ccagggcgtt agtttccctc
     1741 tcgtcgaggc agaaacccag ctcgaagccg cacgacttct ttgttacaaa acactttggt
     1801 tacgagacga agacctacca aattcaaagg aaggtggcat gtgcaagtgg tgggttccgg
     1861 agcttacttt cgaaatcgtt cgtcagtgtt taacgctgca tgggcactat ggttattcga
     1921 ccgaattacc ttttgaacaa cgcctccgtg atatccttgg ctggcagatc ggcgatggga
     1981 catctcagat ctccaagctg cttgtcgcca ggagcatgtt cggtaaggaa tttgcgggtt
     2041 gattgcttgc tcaccatcaa caggctttat taacaaggtc agtcaaattc ttgaggaaat
     2101 gtacgattgc cttttcgtat aacccataga taaagtgagt attggcagca cttttaagac
     2161 catcctgaat tccagcgacc actgcattat cctcctctag accggtctcc gtaacaaatc
     2221 tcgcatcccg tcgcatacgt tcaatatctt cttctgtttc attgccgcta aattgcccga
     2281 gtctgtagga aaagtattgt gtttccgtag gagaaactgg ttctgaaatt acgactgaaa
     2341 tgtggtcgga taaaaacgca acagtacagt ttgggaacac gctataaacg taggttactt
     2401 taccggtgat atctcttttt tcctctgcta tattcctaag atcttctatg cgcctgaatg
     2461 ggaaagtgat tctgctgttt aaacca
//
LOCUS       DASCTH010000326         1598 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000439902, whole genome shotgun sequence.
ACCESSION   DASCTH010000326 DASCTH010000000
VERSION     DASCTH010000326.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000439902"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="QGH35_06770"
     CDS             <1..228
                     /locus_tag="QGH35_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82264.1"
                     /translation="MYRWAKEIAEFYKSKNAGIVQMKNLDGIKEKETYFDRVLNTSWN
                     YSQLQQIIKNKLMENGILKYSPLSRQKTSLF"
     gene            complement(202..1369)
                     /locus_tag="QGH35_06775"
     CDS             complement(join(202..1093,1092..1369))
                     /locus_tag="QGH35_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612067.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HJL82265.1"
                     /translation="MRKRRRYDATFKAKVALEAVKGEKTLAQLCGEYEVHSNQIGLWK
                     KKLIKELPALFSVKGDCEDKNRDELEAELYRQIGKLKVELEWLKKKSQIYSVKKKKAL
                     IEPGHSEIPLFRQCELLGLSRSGYYYRPQGANALNEQLMELIDEQYTKAPFYGVEKMT
                     KWLRRIGFVVNPKRTRRLMRLMGLEAIYPKQRLSIPVGGDKKYPYLLRNLKIDRPDQV
                     WCADITYIRMLQGFVYLVAIMDWYSRYVLAWELSNTMDKQFCLDALERTLETSRPEIF
                     NTDQGSQFTSGEFTGRLEADGIKISMDSRGRFFDNIFIERLWRSVKYEEVYLHGYESV
                     RDAAKGIAKYFQFYNTERLHESLGYRTPQEVYFGVNTNINRQVSLIHLKEACFLS"
     gene            complement(1450..1596)
                     /locus_tag="QGH35_06780"
     CDS             complement(1450..1596)
                     /locus_tag="QGH35_06780"
                     /inference="COORDINATES: protein motif:HMM:NF020463.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HJL82266.1"
                     /translation="MKLEYQNYQHPFYPQTGNDFISHLSVVDLLFNCGEKSLDILLNK
                     TIKS"
BASE COUNT          451 a          384 c          280 g          483 t
ORIGIN      
        1 atgtatagat gggctaaaga gatcgcagaa ttttataaaa gcaaaaatgc cgggatagtt
       61 cagatgaaaa atcttgatgg gattaaagaa aaagagacct attttgacag agttttaaat
      121 acctcttgga attacagtca actccaacaa ataattaaga ataagcttat ggagaacgga
      181 atactaaagt actccccatt atcaagacaa aaaacaagcc tcttttaggt ggattaagct
      241 aacctgtctg ttaatgtttg tattaacacc gaagtagacc tcctgaggag tcctgtaccc
      301 taaggactca tggagtcgtt cggtgttgta aaactgaaaa tattttgcta taccttttgc
      361 ggcatctctt acgctttcat aaccatgaag atacacctct tcatatttaa cacttctcca
      421 tagccgttcg atgaaaatat tgtcgaagaa cctaccgcga ctgtccatgc ttatctttat
      481 tccatcagcc tcaagccttc ctgtaaattc tccgcttgta aattgggaac cttgatcggt
      541 gttaaatatc tcaggacggg aagtctccag cgtcctctcc agagcatcta aacagaactg
      601 tttgtccatc gtgttagaaa gctcccatgc cagtacatac cggctatacc agtccataat
      661 agccacaaga taaacaaacc cttgcaacat acgaatatag gttatgtcag cacaccaaac
      721 ctgatcagga cgatcaatct tcaaattcct caacaggtaa ggatatttct tatctccccc
      781 aacgggaatg ctcaagcgct gctttggata tatcgcctca agccccatca aacgcataag
      841 gcgccttgtt cgcttaggat taaccacaaa gccgatcctt ctaagccatt tcgtcatctt
      901 ctcaactcca taaaaaggag ctttcgtata ctgctcgtcg atgagctcca tcagctgctc
      961 atttaatgcg tttgctccct gtggccgata ataatagccc gatcttgata gtcctaaaag
     1021 ctcacattga cgaaataacg gtatctccga atgtcccggc tctatcaatg cttttttctt
     1081 cttaaccgag tatttgggat ttttttttaa gccattccag ttctaccttc agcttaccta
     1141 tctggcggta aagctctgct tctaactcgt cacggttctt gtcctcgcaa tctcccttca
     1201 cggaaaacag agcaggaagc tccttaataa gtttcttctt ccacagtcca atctggtttg
     1261 aatgaacttc gtattccccg catagctgtg ccagggtctt ttctcccttt accgcctcaa
     1321 gagctacctt agccttgaat gtcgcatcgt accttcttct ctttctcatc ctgtcgtccc
     1381 tcctctaagt tccgacaagt tatcatccac cttaaacctt tgtccagttt ttggggagta
     1441 ttataatcat caagacttga tcgttttgtt aaggagaata tccaagcttt tttcgccaca
     1501 gttaaacaat aaatctacta ctgacaaatg tgaaataaaa tcgttgcctg tctggggata
     1561 aaacggatgc tggtaattct ggtattcaag ttttattg
//
LOCUS       DASCTH010000327         2259 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000427279, whole genome shotgun sequence.
ACCESSION   DASCTH010000327 DASCTH010000000
VERSION     DASCTH010000327.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000427279"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="QGH35_06785"
     CDS             <1..464
                     /locus_tag="QGH35_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HJL82267.1"
                     /translation="GDALVLNYLTPHGKWHIHSTYMENLRMLTLSRGCEPCWLSETDA
                     AKLNIKDNDWVEVYNDHGVYCTRAVVSARIPRGVCIVYHVPERTTGIPKSQIRGNRRA
                     GGHNSVTRIHLKPNYLSGGYGQFSYHFNYWGPIAPNRDTHVTVKKMEKVIF"
     gene            481..2025
                     /gene="narH"
                     /locus_tag="QGH35_06790"
     CDS             481..2025
                     /gene="narH"
                     /locus_tag="QGH35_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HJL82268.1"
                     /translation="MDIRSQISMVFHLDKCIGCHTCSIACKNIWSDRKGAEYMWWNNV
                     ETKPGTGYPGKWENQDIYKGGWEKKGNSITLKGAGKYKGITNIFHNPNLPVIDDYYEP
                     FTYKYLDLIESPADDDQPTARPVSLITGKPMDIKMGPNWDDDLSGTPDYARNDPNLES
                     LSPEEQQAMFQLERMAFFYLPRICNHCLNPACVAACPSGAIYKRGEDGVVLINQSVCR
                     GWRMCVTGCPYKKTYYNWQTGKSEKCILCYPRIEAGHAPACMHSCVGRIRYLGVLLYD
                     ADRLPDVASVDETELVDQQMDMLMDPFDPEVIEQAKQNGVANSTIKAAQQSPIYKFVK
                     EWGMALPLHPEFRTLPMLFYVPPLLPVMASLGKQQSGQKLNDVAKQWDDEWLYDTSTK
                     ELWGTIEDSRFPMQYLANLFSAGDTEKVKNRVKKLMAIRIYRRWKTVGDISEERANEA
                     LNDVDLTAAMADAIYYLTSLAKFNDRFVIPPAHREEAIEMIENTGDRKGSTGFGFKEG
                     STNRGA"
     gene            2027..>2259
                     /locus_tag="QGH35_06795"
     CDS             2027..>2259
                     /locus_tag="QGH35_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJL82269.1"
                     /translation="MSTKTHYSILANLFEYPQEGYPEKVRTVRSQLEDAYPGAIFSLD
                     QFIKLLPEHDLRMMQEIYTRSFDVQAITTLDIG"
BASE COUNT          707 a          457 c          503 g          592 t
ORIGIN      
        1 atggtgatgc tctggtgctc aattatttaa cacctcacgg caagtggcac atccattcca
       61 catatatgga aaatctaagg atgttgactc tatcgcgtgg ttgtgagccc tgctggctca
      121 gcgaaaccga tgcagcaaaa ctcaacatca aagataatga ttgggtggag gtatataacg
      181 accacggcgt ctattgtacc cgagcggtcg tcagtgcaag aattcctcgt ggtgtctgca
      241 ttgtctatca cgtcccagaa agaactactg gcattcccaa atcacaaatt cgcggtaaca
      301 ggcgcgctgg aggtcacaac agtgtcacac ggattcactt gaaacctaac tacctatcgg
      361 gtggttatgg tcaattcagc tatcacttta actactgggg accgattgct ccgaacaggg
      421 acacccatgt tacggtgaag aaaatggaaa aagttatatt ttaaggaggt tatgaaaggc
      481 atggatatac gctcacaaat ttcaatggta ttccacttgg ataagtgcat cggttgtcac
      541 acatgttcta tcgcttgtaa gaacatctgg agtgatcgta aaggtgcaga atacatgtgg
      601 tggaataatg ttgaaacaaa acccggaaca ggttatccag ggaaatggga aaatcaagat
      661 atctataaag gtggctggga gaagaaagga aatagcataa cgctgaaagg tgcaggaaaa
      721 tataaaggca tcaccaacat ttttcataat ccgaacctac ctgtcatcga tgattattac
      781 gaaccattta cttataaata ccttgatctg attgaatctc cggctgatga tgaccagcca
      841 accgcacgac cggtatcgct gatcaccggt aaacccatgg acatcaaaat gggacctaat
      901 tgggatgatg accttagtgg aacacctgat tatgcaagaa acgatcctaa tttagaaagc
      961 ttatctcccg aagaacaaca agccatgttt caactggaac gtatggcttt tttctaccta
     1021 ccgagaattt gtaaccattg cctgaaccct gcctgtgtgg ccgcatgtcc atcaggtgca
     1081 atctataaaa gaggtgaaga tggggtggta ctgatcaatc aaagtgtttg tcgtggttgg
     1141 cgtatgtgtg taacgggttg tccatataag aaaacttact ataactggca aacaggtaaa
     1201 tcagaaaaat gtattctgtg ttacccaaga attgaagctg gtcatgcgcc agcatgcatg
     1261 cacagctgtg tgggtcgtat tcgatatcta ggagttctct tatatgatgc tgatcgacta
     1321 cccgatgtgg catcggttga tgaaactgaa ctcgttgatc aacaaatgga tatgcttatg
     1381 gatccctttg atcctgaggt cattgagcag gcaaaacaaa atggggttgc taattcaacc
     1441 atcaaagctg ctcaacaatc acccatttac aaatttgtta aggagtgggg aatggcgctg
     1501 cctctgcatc cagaatttcg tacattgcca atgttattct atgtcccccc tctccttccc
     1561 gtcatggcct cacttggcaa gcaacaaagt ggccaaaaac tgaatgatgt tgcaaagcaa
     1621 tgggatgatg aatggctata tgatactagc actaaagaac tttggggaac gattgaagat
     1681 tctcgatttc ctatgcaata cctagccaac ctattcagtg caggtgatac agagaaagtg
     1741 aaaaatagag tgaaaaaact gatggctatt agaatttatc gccgttggaa aaccgttggt
     1801 gatatatcag aagaaagagc taacgaggca ttgaatgatg tcgaccttac agccgctatg
     1861 gcagatgcaa tctactactt aacttcactt gcaaaattca atgatagatt tgtaattcct
     1921 cctgcacatc gagaagaagc aattgaaatg atagaaaata cgggtgaccg aaaaggtagt
     1981 accggctttg gtttcaagga aggttcaaca aatagaggag catagtatgt caacaaaaac
     2041 acattattcg attctagcaa atctttttga atacccacaa gagggatacc cggaaaaggt
     2101 tagaaccgta cgttctcagt tagaagatgc ctatcctgga gccatctttt cacttgatca
     2161 attcatcaaa cttttaccag aacatgatct gcgaatgatg caagaaatct atacccgatc
     2221 ttttgatgtg caggctataa caaccttgga tattggtta
//
LOCUS       DASCTH010000328         1793 bp    DNA     linear   ENV 13-DEC-2023
DEFINITION  MAG TPA_asm: Gammaproteobacteria bacterium isolate Gl_MAG_15
            c_000000501882, whole genome shotgun sequence.
ACCESSION   DASCTH010000328 DASCTH010000000
VERSION     DASCTH010000328.1
DBLINK      BioProject: PRJNA955304
            BioSample: SAMN34271064
            Sequence Read Archive: SRR1509792, SRR1509797, SRR1509799,
            SRR1509790, SRR1509793, SRR1509796, SRR1509798, SRR1509800
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gammaproteobacteria bacterium (marine metagenome)
  ORGANISM  Gammaproteobacteria bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Zhang,I.H., Sun,X., Jayakumar,A., Fortin,S.G., Ward,B.B. and
            Babbin,A.R.
  TITLE     Partitioning of the denitrification pathway and other nitrite
            metabolisms within global oxygen deficient zones
  JOURNAL   ISME Commun 3 (1), 76 (2023)
   PUBMED   37474642
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Zhang,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2023) Earth, Atmospheric, and Planetary Sciences,
            Massachusetts Institute of Technology, 77 Massachusetts Avenue,
            Cambridge, MA 02139, USA
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: NOV-2021
            Assembly Method        :: MEGAHIT v. v1.2.9
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 80x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/08/2023 09:34:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,360
            CDSs (total)                      :: 1,326
            Genes (coding)                    :: 1,323
            CDSs (with protein)               :: 1,323
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 1 of 3
            Pseudo Genes (incomplete)         :: 2 of 3
            Pseudo Genes (internal stop)      :: 1 of 3
            Pseudo Genes (multiple problems)  :: 1 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Gammaproteobacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="c_000000501882"
                     /isolate="Gl_MAG_15"
                     /isolation_source="seawater"
                     /db_xref="taxon:1913989"
                     /environmental_sample
                     /country="Pacific Ocean:Eastern Tropical North Pacific
                     ODZ"
                     /lat_lon="18.92 N 104.89 W"
                     /collection_date="2013-06-19"
                     /metagenome_source="marine metagenome"
                     /note="metagenomic"
     gene            <1..>1793
                     /locus_tag="QGH35_06800"
     CDS             <1..>1793
                     /locus_tag="QGH35_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011120560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HJL82270.1"
                     /translation="FAQVSNPSIDSIREKIIMSLECYIGPEQNLLSVTEKHAKRLFIE
                     NPIITFQDIDAIKNIDHYGFQTEVIDITFDVSSSSKENLEQTLDRVCQEVEEAIENGS
                     SFIVLSDRASNQNRAAVSSLLACGAVHHHLVNIAKRTQVGIIIETGEAREVHHHCLLL
                     GYGADAIYPYLAYEAILTAKDERHLQHSHFHPGPENRIPLTDDHSVIRAYRQASVKGI
                     LKVMGKMGISTLHSYKGAQIFETLGLSNDVVNKCFKGTTNRISGVGFETLQAETIRRH
                     QLGWPLQVPQNAERLPNVGDFHWRRAGEKHVWNPETIWPLQKAARTNDLSAYQQFAQH
                     ANAQAEESCTLRGLLKFKQIEENEQLNINEIEPANEIVKRFSTGAMSFGSISSEAHET
                     LAIAMNRIGAKSNSGEGGESRERFQPMPNGDLKSSAIKQIASGRFGVTTTYVANAKQL
                     QIKIAQGAKPGEGGELPGKKVDAKIALTRNSTPGVGLISPPPHHDVYSIEDLAQLIFD
                     LKNANPDAEISVKLVSEAGVGTIAAGVVKAHADHIVISGDVGGTGASPLTSIKHAGLP
                     WELGVAEVQQTLILNNLRDRVKIETDGGIKT"
BASE COUNT          591 a          339 c          390 g          473 t
ORIGIN      
        1 tgtttgccca ggtttcaaac ccatccattg attcaattag agagaaaatt atcatgtctc
       61 tagagtgtta tattggaccg gaacaaaatt tactatcagt tacagaaaag catgccaaac
      121 gattatttat tgagaatccc atcatcactt ttcaagacat tgatgcgatt aagaatattg
      181 accactatgg ttttcagaca gaagttattg atataacatt tgatgtttct tcttcatcaa
      241 aagagaactt agaacagaca ctcgatcggg tttgtcaaga agtggaagag gccattgaaa
      301 atggttcaag ttttattgtg ctttctgacc gagcatcaaa tcaaaaccga gcagctgtga
      361 gtagtctttt ggcttgtggg gctgtccatc atcatctggt aaatattgca aaacgcactc
      421 aagttggaat cattattgag acaggtgaag caagggaagt tcaccatcat tgcttgttat
      481 taggttatgg agcggatgca atttatccct atttagccta cgaagcaatt ttaacagcta
      541 aagatgaaag acatcttcag cattcacatt tccatccagg ccctgaaaac aggataccat
      601 taacagatga tcattctgtc attcgtgctt accgacaagc atcggtaaag ggaattctca
      661 aggtaatggg aaaaatgggg atctccactt tgcactcata caaaggcgca caaatttttg
      721 aaacactagg cctgtccaat gatgttgtta acaagtgctt taaagggact actaatagaa
      781 tatcgggggt cggttttgag actttgcaag cagaaacaat cagaagacac caattaggct
      841 ggcctcttca ggtaccacag aatgccgagc gtttgcctaa tgtgggagat tttcattgga
      901 gaagagctgg agaaaaacat gtttggaacc cagaaacaat ctggcccctg caaaaggctg
      961 ctagaaccaa tgatctcagt gcttatcaac aatttgcaca gcatgcaaat gcccaagcag
     1021 aagaatcctg cacgcttcga ggactcttaa aatttaaaca gatcgaggaa aatgaacaac
     1081 ttaatataaa tgaaatcgag ccagccaatg aaatagttaa acgattttcc accggagcaa
     1141 tgagttttgg atctatctca tcagaagccc atgaaactct agctattgca atgaatcgta
     1201 ttggagctaa aagcaacagt ggagaaggag gtgagagccg tgaaagattt cagcctatgc
     1261 ccaacgggga tttaaaatcg tcagcaatta agcaaattgc ttcaggtcgt tttggtgtca
     1321 caacaaccta tgttgcaaat gccaaacaat tacaaattaa gatagctcaa ggagcaaaac
     1381 caggtgaagg aggggagctt cctgggaaaa aagttgatgc aaaaatagcc ttaactagaa
     1441 attccactcc aggtgttggc ttgataagtc ctcctccaca tcatgatgtt tattctattg
     1501 aagatttagc acagttgatt tttgacctca aaaatgccaa tcctgatgca gaaattagcg
     1561 tgaagttagt ctcggaagca ggagtcggaa ccattgcagc aggagtggtc aaagcacatg
     1621 ctgatcatat cgtcatttct ggtgatgttg gtgggacggg cgcctcaccg ctaacaagta
     1681 taaagcatgc agggcttcct tgggaattag gcgtcgccga agtgcaacaa actttaattt
     1741 taaataattt aagagatagg gtaaaaattg aaacagatgg aggaataaaa act
//
